Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,598	A→G	V82A (GTC→GCC)	dsbC ←	disulfide isomerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,598	0	A	G	99.6%	953.5 / NA	273	V82A (GTC→GCC)	dsbC	disulfide isomerase II
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (131/141); total (131/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

ACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTA  >  NC_000913/3036508‑3036687

acGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCa                                                                                         <  1:1732652/93‑1 (MQ=255)

  cgttatgaCGTTTTTTTCCTGCGGCCCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCAtt                                                                                       <  1:137454/89‑1 (MQ=255)

   gTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTg                                                                                      <  1:5687558/93‑1 (MQ=255)

   gTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTg                                                                                      >  2:2248483/1‑93 (MQ=255)

    tgatgaCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGa                                                                                     <  1:4449039/93‑1 (MQ=255)

    tgatgaCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGa                                                                                     <  2:4011473/93‑1 (MQ=255)

     gatgaCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGAc                                                                                    >  2:4254214/1‑93 (MQ=255)

      atgaCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGAcc                                                                                   <  2:4122917/93‑1 (MQ=255)

        gaCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCgg                                                                                 >  1:1992400/1‑93 (MQ=255)

         aCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGa                                                                                >  1:5865002/1‑93 (MQ=255)

         aCGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGa                                                                                <  2:5958190/93‑1 (MQ=255)

          cGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAg                                                                               >  2:2457676/1‑93 (MQ=255)

          cGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAg                                                                               <  1:305871/93‑1 (MQ=255)

           gtgtTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGc                                                                              <  1:1496355/93‑1 (MQ=255)

                   ccTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCAc                                                                      >  1:3814031/1‑93 (MQ=255)

                   ccTGCGGCGCTTTATAAACGATCATCTCTTTTCCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCAc                                                                      <  1:2607686/93‑1 (MQ=255)

                    cTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACt                                                                     >  2:4475740/1‑93 (MQ=255)

                    cTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACt                                                                     >  1:4458395/1‑93 (MQ=255)

                     tGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTa                                                                    >  1:6013171/1‑93 (MQ=255)

                      gcggcgCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTaa                                                                   >  1:839455/1‑93 (MQ=255)

                           gcTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCa                                                              <  1:3416676/93‑1 (MQ=255)

                           gcTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCa                                                              >  1:5241983/1‑93 (MQ=255)

                           gcTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCa                                                              <  2:93241/93‑1 (MQ=255)

                           gcTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCa                                                              <  2:603469/93‑1 (MQ=255)

                            cTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCAt                                                             >  2:5057237/1‑93 (MQ=255)

                             tttATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATa                                                            <  2:485360/93‑1 (MQ=255)

                              ttATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATAc                                                           <  2:4327721/93‑1 (MQ=255)

                              ttATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATAc                                                           <  2:4788321/93‑1 (MQ=255)

                               tataAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACa                                                          >  2:2999329/1‑93 (MQ=255)

                                ataAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACAt                                                         <  2:5633756/93‑1 (MQ=255)

                                  aaaCGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTg                                                       <  1:1540309/93‑1 (MQ=255)

                                    aCGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGc                                                     >  2:2385790/1‑93 (MQ=255)

                                    aCGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGc                                                     >  1:1248047/1‑93 (MQ=255)

                                     cGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGcc                                                    <  2:1089555/93‑1 (MQ=255)

                                      gATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGccc                                                   <  2:362660/93‑1 (MQ=255)

                                      gATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGccc                                                   >  2:2664973/1‑93 (MQ=255)

                                      gATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGccc                                                   >  2:549613/1‑93 (MQ=255)

                                      gATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGccc                                                   >  1:4640888/1‑93 (MQ=255)

                                      gATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGccc                                                   >  1:1045075/1‑93 (MQ=255)

                                       atcatcTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGcccc                                                  >  2:3202488/1‑93 (MQ=255)

                                       atcatcTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGcccc                                                  <  1:1080107/93‑1 (MQ=255)

                                          atcTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGa                                               >  2:3811309/1‑93 (MQ=255)

                                           tctcTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGaa                                              >  1:5758148/1‑93 (MQ=255)

                                             tctTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATg                                            >  1:98045/1‑93 (MQ=255)

                                              ctTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCCTACATTGGCCCCTGAATgc                                           >  2:1392795/1‑92 (MQ=255)

                                                ttttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGgta                                         >  2:1979333/1‑93 (MQ=255)

                                                ttttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGata                                         <  1:4109082/93‑1 (MQ=255)

                                                 tttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGatat                                        <  2:3275735/93‑1 (MQ=255)

                                                 tttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGatat                                        <  2:3099169/93‑1 (MQ=255)

                                                  ttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATg                                       <  2:5357035/93‑1 (MQ=255)

                                                  ttCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATg                                       <  1:3904888/93‑1 (MQ=255)

                                                   tCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGt                                      <  1:2765807/93‑1 (MQ=255)

                                                    cAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGtt                                     <  1:6362841/93‑1 (MQ=255)

                                                    cAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGtt                                     <  1:3965708/93‑1 (MQ=255)

                                                     aaGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGttt                                    >  1:3493416/1‑93 (MQ=255)

                                                     aaGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGttt                                    >  2:1739063/1‑93 (MQ=255)

                                                     aaGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGttt                                    >  2:1227921/1‑93 (MQ=255)

                                                      aGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTa                                   <  1:3665106/93‑1 (MQ=255)

                                                      aGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTa                                   <  1:490924/93‑1 (MQ=255)

                                                      aGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTa                                   <  1:4946038/93‑1 (MQ=255)

                                                       gcgcATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAc                                  <  2:645784/93‑1 (MQ=255)

                                                        cgcATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAcc                                 <  2:584299/93‑1 (MQ=255)

                                                          cATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcat                               <  1:2963826/93‑1 (MQ=255)

                                                          cATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcat                               <  1:4328237/93‑1 (MQ=255)

                                                           aTTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcatc                              >  1:716881/1‑93 (MQ=255)

                                                           aTTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcatc                              >  1:5412786/1‑93 (MQ=255)

                                                            ttCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcatca                             <  1:234240/93‑1 (MQ=255)

                                                             tCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACcatcat                            >  1:549750/1‑93 (MQ=255)

                                                              cAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCatcatc                           <  1:2426246/93‑1 (MQ=255)

                                                              cAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCatcatc                           <  2:4892430/93‑1 (MQ=255)

                                                              cAACTGCTTTAACAGCATCTTATTGGTGGCATTCACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCatcatc                           <  2:5314318/93‑1 (MQ=255)

                                                              cAACGGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATCCATTGGCCCCTGAATGATATGTTTACCatcatc                           <  2:790240/93‑1 (MQ=255)

                                                               aaCTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCg                          >  2:3683501/1‑93 (MQ=255)

                                                               aaCTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCg                          <  1:4850254/93‑1 (MQ=255)

                                                                aCTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCgg                         >  2:4523305/1‑93 (MQ=255)

                                                                aCTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCgg                         >  2:1541132/1‑93 (MQ=255)

                                                                 cTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGt                        <  1:4213384/93‑1 (MQ=255)

                                                                  tGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTg                       >  2:6339407/1‑93 (MQ=255)

                                                                   gCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGa                      <  1:4494013/93‑1 (MQ=255)

                                                                   gCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGa                      <  2:1904490/93‑1 (MQ=255)

                                                                    cTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAt                     >  1:4101606/1‑93 (MQ=255)

                                                                    cTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAt                     >  2:2068584/1‑93 (MQ=255)

                                                                    cTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAt                     >  1:3185660/1‑93 (MQ=255)

                                                                     tttAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATg                    <  1:5652735/93‑1 (MQ=255)

                                                                     tttAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATg                    >  1:5093697/1‑93 (MQ=255)

                                                                     tttAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATg                    <  2:3150321/93‑1 (MQ=255)

                                                                         aCAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTaca                >  2:5269766/1‑93 (MQ=255)

                                                                          cAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTacaa               <  2:3437945/93‑1 (MQ=255)

                                                                           aGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTacaac              >  2:5155415/1‑93 (MQ=255)

                                                                            gCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTacaaca             >  1:4404428/1‑93 (MQ=255)

                                                                            gCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTacaaca             <  2:2553461/93‑1 (MQ=255)

                                                                            gCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTacaaca             >  1:194771/1‑93 (MQ=255)

                                                                              aTCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAcg           >  1:455812/1‑93 (MQ=255)

                                                                                cTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAcgcc         <  2:3354287/93‑1 (MQ=255)

                                                                                  tATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAcgccgc       <  2:4958749/93‑1 (MQ=255)

                                                                                   aTTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCt      <  1:2121508/93‑1 (MQ=255)

                                                                                   aTTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCt      <  1:5372456/93‑1 (MQ=255)

                                                                                    ttGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTg     <  2:833900/93‑1 (MQ=255)

                                                                                     tggtggCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGt    <  1:789940/93‑1 (MQ=255)

                                                                                       gTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTa  >  2:5655484/1‑93 (MQ=255)

ACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTA  >  NC_000913/3036508‑3036687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTAG  >  NC_000913/3036506‑3036688

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGC                                                                                           <  SRR4302537.6079365/94‑1 (MQ=60)

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGC                                                                                           <  SRR4302537.1141077/94‑1 (MQ=60)

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGC                                                                                           >  SRR4302537.428669/1‑94 (MQ=60)

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGC                                                                                           >  SRR4302537.1327822/1‑94 (MQ=60)

 CACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCA                                                                                          <  SRR4302537.1852144/94‑1 (MQ=60)

  ACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCAT                                                                                         <  SRR4302537.1744744/94‑1 (MQ=60)

  ACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCAT                                                                                         <  SRR4302537.4959211/94‑1 (MQ=60)

   CGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATT                                                                                        <  SRR4302537.303610/94‑1 (MQ=60)

    cgttATGACGTTTTTTTCCTGCGGCCCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTG                                                                                       <  SRR4302537.138441/90‑1 (MQ=60)

     GTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGA                                                                                      <  SRR4302537.3494886/94‑1 (MQ=60)

     GTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGA                                                                                      <  SRR4302537.5726461/94‑1 (MQ=60)

     GTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGA                                                                                      >  SRR4302537.4958696/1‑94 (MQ=60)

      TGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGAC                                                                                     <  SRR4302537.6244543/94‑1 (MQ=60)

      TGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGAC                                                                                     <  SRR4302537.4453717/94‑1 (MQ=60)

      TGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGAC                                                                                     <  SRR4302537.4479764/94‑1 (MQ=60)

       GATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACC                                                                                    >  SRR4302537.5040653/1‑94 (MQ=60)

        ATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGAATTGACCG                                                                                   >  SRR4302537.1351675/1‑94 (MQ=60)

         TGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGG                                                                                  >  SRR4302537.2006255/1‑94 (MQ=60)

          GACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGA                                                                                 >  SRR4302537.5905229/1‑94 (MQ=60)

          GACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGA                                                                                 >  SRR4302537.449315/1‑94 (MQ=60)

          GACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGA                                                                                 <  SRR4302537.1100828/94‑1 (MQ=60)

           ACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAG                                                                                <  SRR4302537.2926917/94‑1 (MQ=60)

           ACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAG                                                                                >  SRR4302537.590018/1‑94 (MQ=60)

            CGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGC                                                                               <  SRR4302537.307991/94‑1 (MQ=60)

            CGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGC                                                                               >  SRR4302537.5079075/1‑94 (MQ=60)

             GTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCC                                                                              <  SRR4302537.1506790/94‑1 (MQ=60)

             GTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCC                                                                              <  SRR4302537.876507/94‑1 (MQ=60)

                TTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTG                                                                           <  SRR4302537.4086491/94‑1 (MQ=60)

                    TCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCAC                                                                       >  SRR4302537.3840776/1‑94 (MQ=60)

                     CCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACT                                                                      >  SRR4302537.4489222/1‑94 (MQ=60)

                     CCTGCGGCGCTTTATAAACGATCATCTCTTTTCCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACT                                                                      <  SRR4302537.2625920/94‑1 (MQ=60)

                      CTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTA                                                                     >  SRR4302537.6054483/1‑94 (MQ=60)

                       TGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAA                                                                    >  SRR4302537.845205/1‑94 (MQ=60)

                            CGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCA                                                               >  SRR4302537.4327850/1‑94 (MQ=60)

                            CGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCA                                                               >  SRR4302537.5278058/1‑94 (MQ=60)

                            CGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCA                                                               >  SRR4302537.2408565/1‑94 (MQ=60)

                             GCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCAT                                                              <  SRR4302537.3926177/94‑1 (MQ=60)

                             GCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCAT                                                              <  SRR4302537.3440866/94‑1 (MQ=60)

                              CTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATA                                                             >  SRR4302537.5410897/1‑94 (MQ=60)

                                TTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACA                                                           >  SRR4302537.4226599/1‑94 (MQ=60)

                                TTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACA                                                           <  SRR4302537.4529622/94‑1 (MQ=60)

                                 TATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACAT                                                          <  SRR4302537.1667990/94‑1 (MQ=60)

                                 TATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACAT                                                          <  SRR4302537.5496176/94‑1 (MQ=60)

                                 TATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACAT                                                          >  SRR4302537.1812422/1‑94 (MQ=60)

                                 TATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACAT                                                          <  SRR4302537.3505585/94‑1 (MQ=60)

                                   TAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTG                                                        <  SRR4302537.5561452/94‑1 (MQ=60)

                                    AAGCGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGG                                                       <  SRR4302537.4832195/94‑1 (MQ=60)

                                    AAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGG                                                       <  SRR4302537.5308200/94‑1 (MQ=60)

                                    AAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGG                                                       <  SRR4302537.1551058/94‑1 (MQ=60)

                                     AACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGC                                                      >  SRR4302537.1256740/1‑94 (MQ=60)

                                     AACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGC                                                      <  SRR4302537.1229122/94‑1 (MQ=60)

                                       CGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCC                                                    >  SRR4302537.4672979/1‑94 (MQ=60)

                                       CGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCC                                                    >  SRR4302537.1052287/1‑94 (MQ=60)

                                       CGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCC                                                    <  SRR4302537.5755324/94‑1 (MQ=60)

                                        GATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCC                                                   >  SRR4302537.3136086/1‑94 (MQ=60)

                                        GATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCC                                                   >  SRR4302537.1989123/1‑94 (MQ=60)

                                         ATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCT                                                  >  SRR4302537.5725786/1‑94 (MQ=60)

                                         ATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCT                                                  <  SRR4302537.1664200/94‑1 (MQ=60)

                                         ATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCT                                                  <  SRR4302537.1087562/94‑1 (MQ=60)

                                         ATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCT                                                  >  SRR4302537.589416/1‑94 (MQ=60)

                                         ATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCT                                                  >  SRR4302537.4505950/1‑94 (MQ=60)

                                          TCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTG                                                 >  SRR4302537.493694/1‑94 (MQ=60)

                                          TCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTG                                                 >  SRR4302537.4616563/1‑94 (MQ=60)

                                           CATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGA                                                <  SRR4302537.264326/94‑1 (MQ=60)

                                           CATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGA                                                >  SRR4302537.727604/1‑94 (MQ=60)

                                            ATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAA                                               <  SRR4302537.2388281/94‑1 (MQ=60)

                                            ATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAA                                               >  SRR4302537.5219398/1‑94 (MQ=60)

                                            ATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAA                                               <  SRR4302537.3422284/94‑1 (MQ=60)

                                            ATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAA                                               >  SRR4302537.5797589/1‑94 (MQ=60)

                                             TCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAAT                                              <  SRR4302537.521816/94‑1 (MQ=60)

                                              CTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATG                                             >  SRR4302537.98757/1‑94 (MQ=60)

                                              CTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATG                                             >  SRR4302537.2048492/1‑94 (MQ=60)

                                               TCTTTTTCAAGCGCATTAAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGA                                            <  SRR4302537.810358/94‑1 (MQ=60)

                                                CTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGAT                                           >  SRR4302537.322406/1‑94 (MQ=60)

                                                 TTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATA                                          >  SRR4302537.252429/1‑94 (MQ=60)

                                                  TTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATAT                                         <  SRR4302537.4137792/94‑1 (MQ=60)

                                                  TTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATAT                                         <  SRR4302537.2132135/94‑1 (MQ=60)

                                                    TTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGT                                       <  SRR4302537.3932189/94‑1 (MQ=60)

                                                    TTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGT                                       >  SRR4302537.454804/1‑94 (MQ=60)

                                                     TCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTT                                      <  SRR4302537.2785134/94‑1 (MQ=60)

                                                      CAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTT                                     <  SRR4302537.3993450/94‑1 (MQ=60)

                                                      CAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTT                                     >  SRR4302537.442122/1‑94 (MQ=60)

                                                      CAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTT                                     <  SRR4302537.6406249/94‑1 (MQ=60)

                                                      CAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTT                                     >  SRR4302537.3518127/1‑94 (MQ=60)

                                                       AAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTA                                    <  SRR4302537.1059132/94‑1 (MQ=60)

                                                        AGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAC                                   <  SRR4302537.3690906/94‑1 (MQ=60)

                                                        AGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAC                                   <  SRR4302537.4980142/94‑1 (MQ=60)

                                                        AGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAC                                   >  SRR4302537.4135098/1‑94 (MQ=60)

                                                        AGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTAC                                   <  SRR4302537.494297/94‑1 (MQ=60)

                                                         GCGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACC                                  <  SRR4302537.3858198/94‑1 (MQ=60)

                                                          CGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCA                                 >  SRR4302537.5489571/1‑94 (MQ=60)

                                                          CGCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCA                                 >  SRR4302537.1766538/1‑94 (MQ=60)

                                                           GCATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCAT                                <  SRR4302537.838458/94‑1 (MQ=60)

                                                            CATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATC                               >  SRR4302537.5449693/1‑94 (MQ=60)

                                                            CATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATC                               >  SRR4302537.6123557/1‑94 (MQ=60)

                                                            CATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATC                               <  SRR4302537.4358317/94‑1 (MQ=60)

                                                            CATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATC                               <  SRR4302537.2984627/94‑1 (MQ=60)

                                                            CATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATC                               >  SRR4302537.721778/1‑94 (MQ=60)

                                                             ATTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCA                              <  SRR4302537.3576317/94‑1 (MQ=60)

                                                              TTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCAT                             <  SRR4302537.235896/94‑1 (MQ=60)

                                                              TTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCAT                             >  SRR4302537.3163201/1‑94 (MQ=60)

                                                              TTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCAT                             <  SRR4302537.4369288/94‑1 (MQ=60)

                                                              TTCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCAT                             >  SRR4302537.553533/1‑94 (MQ=60)

                                                              ATCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCAT                             <  SRR4302537.1528400/94‑1 (MQ=60)

                                                               TCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATC                            <  SRR4302537.5337510/94‑1 (MQ=60)

                                                               TCAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATC                            <  SRR4302537.2539894/94‑1 (MQ=60)

                                                                TAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCG                           <  SRR4302537.3811833/94‑1 (MQ=60)

                                                                CAACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCG                           <  SRR4302537.2443339/94‑1 (MQ=60)

                                                                 AACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGG                          <  SRR4302537.4883722/94‑1 (MQ=60)

                                                                  ACTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGT                         <  SRR4302537.1388415/94‑1 (MQ=60)

                                                                   CTGCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTG                        <  SRR4302537.4242768/94‑1 (MQ=60)

                                                                     GCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAT                      >  SRR4302537.3208065/1‑94 (MQ=60)

                                                                     GCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAT                      >  SRR4302537.4130244/1‑94 (MQ=60)

                                                                     GCTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGAT                      <  SRR4302537.4525102/94‑1 (MQ=60)

                                                                      CTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATG                     >  SRR4302537.5637884/1‑94 (MQ=60)

                                                                      CTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATG                     >  SRR4302537.5128769/1‑94 (MQ=60)

                                                                      CTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATG                     >  SRR4302537.942895/1‑94 (MQ=60)

                                                                      CTTTAACAGCATCTTATTGGTGGCATTGACCGGAGCAGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATG                     >  SRR4302537.4344513/1‑94 (MQ=60)

                                                                       TTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGT                    >  SRR4302537.6041510/1‑94 (MQ=60)

                                                                       TTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGT                    <  SRR4302537.5691419/94‑1 (MQ=60)

                                                                       TTTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGT                    >  SRR4302537.2289815/1‑94 (MQ=60)

                                                                       TATAACAGCATCTTATTTGTGACATTGACCGGAGCCGTGCCACTAACGTCATTCATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGT                    <  SRR4302537.5691420/94‑1 (MQ=60)

                                                                        TTAACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTA                   >  SRR4302537.1287082/1‑94 (MQ=60)

                                                                           ACAGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAA                >  SRR4302537.477053/1‑94 (MQ=60)

                                                                            CAGCATCTTATTAGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAAC               <  SRR4302537.5932587/94‑1 (MQ=60)

                                                                             AGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACA              >  SRR4302537.4434819/1‑94 (MQ=60)

                                                                             AGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACA              >  SRR4302537.6449714/1‑94 (MQ=60)

                                                                             AGCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACA              >  SRR4302537.196155/1‑94 (MQ=60)

                                                                              GCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAC             <  SRR4302537.4457811/94‑1 (MQ=60)

                                                                              GCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAC             <  SRR4302537.6469357/94‑1 (MQ=60)

                                                                              GCATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTCGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACAC             <  SRR4302537.4590661/94‑1 (MQ=60)

                                                                               CATCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACG            >  SRR4302537.458957/1‑94 (MQ=60)

                                                                                 TCTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCC          <  SRR4302537.2878886/94‑1 (MQ=60)

                                                                                  TTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCG         <  SRR4302537.4484756/94‑1 (MQ=60)

                                                                                  CTTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCG         <  SRR4302537.4123686/94‑1 (MQ=60)

                                                                                   TTATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGC        <  SRR4302537.4173290/94‑1 (MQ=60)

                                                                                    TATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCT       >  SRR4302537.1449753/1‑94 (MQ=60)

                                                                                    TATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCT       <  SRR4302537.3523779/94‑1 (MQ=60)

                                                                                     ATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTG      >  SRR4302537.1976848/1‑94 (MQ=60)

                                                                                     ATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTG      <  SRR4302537.2136437/94‑1 (MQ=60)

                                                                                     ATTGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTG      <  SRR4302537.5409202/94‑1 (MQ=60)

                                                                                       TGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTT    <  SRR4302537.795361/94‑1 (MQ=60)

                                                                                       TGGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTT    >  SRR4302537.3966078/1‑94 (MQ=60)

                                                                                        GGTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTA   <  SRR4302537.2693547/94‑1 (MQ=60)

                                                                                         GTGGCATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTAG  <  SRR4302537.3982085/94‑1 (MQ=60)

ACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTAG  >  NC_000913/3036506‑3036688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`