Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,058,415	A→G	S215G (AGC→GGC)	iciA →	replication initiation inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,058,415	0	A	G	100.0%	770.4 / NA	221	S215G (AGC→GGC)	iciA	replication initiation inhibitor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (132/89); total (132/89)

Only 100 of 241 total aligned reads displayed.

GCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCAGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAA  >  NC_000913/3058325‑3058504

gCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAggcggc                                                                                          >  2:6270073/1‑93 (MQ=255)

  aTTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGt                                                                                        >  2:2727070/1‑93 (MQ=255)

   ttACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTg                                                                                       >  2:2147882/1‑93 (MQ=255)

   ttACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTg                                                                                       >  1:5235143/1‑93 (MQ=255)

         aaaGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGc                                                                                <  1:5561523/93‑1 (MQ=255)

          aaGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGcc                                                                               <  1:4471511/93‑1 (MQ=255)

           aGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCa                                                                              >  2:6125264/1‑93 (MQ=255)

            gcgcCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCat                                                                             <  2:3007572/93‑1 (MQ=255)

            gcgcCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCat                                                                             <  1:2086509/93‑1 (MQ=255)

              gcCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCatat                                                                           >  2:51743/1‑93 (MQ=255)

                cAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCg                                                                         >  1:4128594/1‑93 (MQ=255)

                cAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCg                                                                         >  2:4809163/1‑93 (MQ=255)

                 cgTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGt                                                                        <  1:1874350/92‑1 (MQ=255)

                 aGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGt                                                                        >  1:4494823/1‑93 (MQ=255)

                 aGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGt                                                                        <  2:333454/93‑1 (MQ=255)

                    ggTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTaa                                                                     <  1:5639836/93‑1 (MQ=255)

                     gTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAt                                                                    >  1:2767863/1‑93 (MQ=255)

                     gTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAt                                                                    >  2:3659126/1‑93 (MQ=255)

                     gTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAt                                                                    <  2:2348165/93‑1 (MQ=255)

                        gcgTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAttct                                                                 <  2:5533886/93‑1 (MQ=255)

                        gcgTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAttct                                                                 <  1:3502684/93‑1 (MQ=255)

                        gcgTGTGACTATCTTGACAATAGGCAGCAGGCTGTTTTGCGGCAAAGTTTCGATCTCCCTCAAGGC‑GGGTGACCCTGCCATATCGTTAAttct                                                                 <  2:5591507/93‑1 (MQ=255)

                         cgTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAttctt                                                                >  2:4214930/1‑93 (MQ=255)

                          gTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAttcttc                                                               <  2:1220312/93‑1 (MQ=255)

                          gTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAAttcttc                                                               >  2:1057851/1‑93 (MQ=255)

                           tttGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCa                                                              >  2:687797/1‑93 (MQ=255)

                              gACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGaa                                                           >  1:1297444/1‑93 (MQ=255)

                              gACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGaa                                                           >  1:1529336/1‑93 (MQ=255)

                               aCCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAg                                                          >  1:3435888/1‑93 (MQ=255)

                               aCCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAg                                                          <  2:1647402/93‑1 (MQ=255)

                                ccATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGc                                                         >  2:2847059/1‑93 (MQ=255)

                                ccATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGc                                                         >  2:4421505/1‑93 (MQ=255)

                                 cATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCg                                                        <  1:5813883/93‑1 (MQ=255)

                                 cATCTTGACGAGATGCACGAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCg                                                        <  2:6145233/93‑1 (MQ=255)

                                  aTCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGt                                                       <  1:983141/93‑1 (MQ=255)

                                  aTCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGt                                                       >  1:565957/1‑93 (MQ=255)

                                  aTCTTGACGATATGCACCAGGCCTTTTCGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGt                                                       <  1:4085677/93‑1 (MQ=255)

                                   tCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGtt                                                      >  1:1717082/1‑93 (MQ=255)

                                   tCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGtt                                                      <  1:1690672/93‑1 (MQ=255)

                                   tCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGtt                                                      <  1:4615996/93‑1 (MQ=255)

                                    cTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGttt                                                     <  1:6096869/93‑2 (MQ=255)

                                     ttGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCg                                                    >  1:2554904/1‑93 (MQ=255)

                                        aCGATATGCACCAGGCCTTTTTGCAGCAAACCTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTAc                                                 <  2:4446855/93‑1 (MQ=255)

                                        aCGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTAc                                                 <  1:2172186/93‑1 (MQ=255)

                                         cGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACa                                                >  1:6425881/1‑93 (MQ=255)

                                          gATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATCTCGTTAATTCTTCAGAAGCGTTCGTACaa                                               >  1:691881/1‑93 (MQ=255)

                                          gATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGGGTTCGTACaa                                               <  1:5035959/93‑1 (MQ=255)

                                             atGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACtt                                            >  1:5123482/1‑93 (MQ=255)

                                                 aCCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTc                                        <  2:1871398/93‑1 (MQ=255)

                                                 aCCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTc                                        >  1:2608126/1‑93 (MQ=255)

                                                  ccAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCg                                       >  1:233148/1‑93 (MQ=255)

                                                  ccAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCg                                       >  1:4368130/1‑93 (MQ=255)

                                                   cAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGc                                      >  2:4624086/1‑93 (MQ=255)

                                                   cAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGc                                      >  1:1124793/1‑93 (MQ=255)

                                                   cAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGAGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGc                                      >  2:380574/1‑93 (MQ=255)

                                                    aGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGcc                                     <  1:6117707/93‑1 (MQ=255)

                                                    aGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGcc                                     >  2:3564209/1‑93 (MQ=255)

                                                     ggCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCa                                    >  1:863142/1‑93 (MQ=255)

                                                     ggCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCa                                    <  2:1304393/93‑1 (MQ=255)

                                                     ggCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCa                                    <  1:1368079/93‑1 (MQ=255)

                                                     gacttttttGCAGAAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCa                                    <  2:3449068/89‑1 (MQ=255)

                                                      gCCTTTTTGCAGCAAATCTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAg                                   <  1:1583207/93‑1 (MQ=255)

                                                      gCCTTTTTGCAGCAAACCGTCGATCTGCCTACAGCAGGCGAG‑CCCTGCCATATCGTTAAGTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAg                                   <  2:495463/93‑1 (MQ=255)

                                                      gCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAg                                   >  2:1467596/1‑93 (MQ=255)

                                                       ccTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAgg                                  >  1:3047669/1‑93 (MQ=255)

                                                       ccTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAgg                                  >  2:837076/1‑93 (MQ=255)

                                                         tttttGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGc                                >  1:3798577/1‑93 (MQ=255)

                                                         tttttGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGc                                <  2:4125952/93‑1 (MQ=255)

                                                            ttGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGaacc                             <  2:4860694/93‑1 (MQ=255)

                                                              gcagcaAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGcaccac                           <  1:4900852/93‑1 (MQ=255)

                                                                 gcaAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACctg                        >  2:3376719/1‑93 (MQ=255)

                                                                 gcaAAACTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACctg                        >  1:2035193/1‑93 (MQ=255)

                                                                     aaCTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGt                    >  1:4645575/1‑93 (MQ=255)

                                                                       ctacGATCTGCCCCCAGGCGGCGTC‑CCCTGCCATATCGTTAATTCTTCAGAAGAGTTCGTACCACTTGCTCGCCAGGGCACCACCTGCTGTAt                  <  1:2910645/90‑1 (MQ=255)

                                                                       cTTCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTAt                  <  1:1179161/93‑1 (MQ=255)

                                                                        ttCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATg                 <  1:1541379/93‑1 (MQ=255)

                                                                         tCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGa                <  1:6305141/93‑1 (MQ=255)

                                                                         tCGATCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGa                <  1:5419086/93‑1 (MQ=255)

                                                                          cGATCTGCCTCCAGGCGCCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGAt               <  1:4044347/93‑1 (MQ=255)

                                                                            aTCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATcc             >  1:5455555/1‑93 (MQ=255)

                                                                             tCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATccc            >  1:5367627/1‑93 (MQ=255)

                                                                             tCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATccc            >  1:2457841/1‑93 (MQ=255)

                                                                             tCTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATccc            >  2:571538/1‑93 (MQ=255)

                                                                              cTGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCg           >  1:5173493/1‑93 (MQ=255)

                                                                               tGCCTCCAGGCGGCGTG‑GCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGAGCCCGc          >  2:5207854/1‑93 (MQ=255)

                                                                               tGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGc          >  1:3844861/1‑93 (MQ=255)

                                                                               tGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGc          >  2:3113402/1‑93 (MQ=255)

                                                                               tGCCTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGc          <  2:2527818/93‑1 (MQ=255)

                                                                                  cTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCAcc       >  2:5895557/1‑93 (MQ=255)

                                                                                  cTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCAcc       >  1:1018378/1‑93 (MQ=255)

                                                                                  cTCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGCATGATCCCGCAcc       >  1:6199223/1‑93 (MQ=255)

                                                                                   tCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCt      <  1:5954151/93‑1 (MQ=255)

                                                                                   tCCAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCt      >  2:958599/1‑93 (MQ=255)

                                                                                   cccAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCt      <  1:3600133/92‑1 (MQ=255)

                                                                                   cccAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCt      <  2:6466538/92‑1 (MQ=255)

                                                                                    ccAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTg     >  2:4532444/1‑93 (MQ=255)

                                                                                     cAGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGc    >  1:5769421/1‑93 (MQ=255)

                                                                                      aGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCa   <  1:4699834/93‑1 (MQ=255)

                                                                                      aGGCGGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCa   >  2:6351016/1‑93 (MQ=255)

                                                                                       ggcggcGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATGCCGCACCCGCaa  >  1:3258814/1‑93 (MQ=255)

GCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCAGCGTG‑CCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAA  >  NC_000913/3058325‑3058504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCAGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAAATCG  >  NC_000913/3058322‑3058508

TCGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCG                                                                                               >  SRR4302537.5219947/1‑94 (MQ=60)

TCGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCG                                                                                               <  SRR4302537.1419485/94‑1 (MQ=60)

 CGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGG                                                                                              >  SRR4302537.1084099/1‑94 (MQ=60)

 CGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGG                                                                                              >  SRR4302537.3855690/1‑94 (MQ=60)

 CGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGG                                                                                              >  SRR4302537.1670044/1‑94 (MQ=60)

 CGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGG                                                                                              <  SRR4302537.1348900/94‑1 (MQ=60)

  GGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGC                                                                                             <  SRR4302537.1433831/94‑1 (MQ=60)

    CATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGT                                                                                           >  SRR4302537.616120/1‑94 (MQ=60)

     ATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG                                                                                          <  SRR4302537.1858464/94‑1 (MQ=60)

     ATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTG                                                                                          >  SRR4302537.5271160/1‑94 (MQ=60)

      TTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGC                                                                                         <  SRR4302537.5091882/94‑1 (MQ=60)

      TTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGC                                                                                         >  SRR4302537.4985794/1‑94 (MQ=60)

       TACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCC                                                                                        >  SRR4302537.6201727/1‑94 (MQ=60)

         CTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCT                                                                                      >  SRR4302537.3718838/1‑94 (MQ=60)

            AAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCC                                                                                   <  SRR4302537.6155458/94‑1 (MQ=60)

            AAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCC                                                                                   <  SRR4302537.5599528/94‑1 (MQ=60)

            AAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCC                                                                                   >  SRR4302537.3420319/1‑94 (MQ=60)

             AAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCA                                                                                  <  SRR4302537.4502441/94‑1 (MQ=60)

             AAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCA                                                                                  <  SRR4302537.2992638/94‑1 (MQ=60)

              AGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCAT                                                                                 >  SRR4302537.4143068/1‑94 (MQ=60)

              AGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCAT                                                                                 >  SRR4302537.569528/1‑94 (MQ=60)

               GCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATA                                                                                <  SRR4302537.5936283/94‑1 (MQ=60)

               GCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATA                                                                                <  SRR4302537.2101220/94‑1 (MQ=60)

                CGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATAT                                                                               >  SRR4302537.1718312/1‑94 (MQ=60)

                 GCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATC                                                                              >  SRR4302537.1690267/1‑94 (MQ=60)

                  CCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCG                                                                             >  SRR4302537.4157410/1‑94 (MQ=60)

                  CCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCG                                                                             >  SRR4302537.3028793/1‑94 (MQ=60)

                   CAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGT                                                                            >  SRR4302537.4525915/1‑94 (MQ=60)

                    CGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTT                                                                           <  SRR4302537.1887392/94‑1 (MQ=60)

                      TGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAA                                                                         <  SRR4302537.4667263/94‑1 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        >  SRR4302537.1092530/1‑94 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        >  SRR4302537.2787207/1‑94 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        <  SRR4302537.5318770/94‑1 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        >  SRR4302537.5445126/1‑94 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        <  SRR4302537.5678431/94‑1 (MQ=60)

                       GGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAAT                                                                        >  SRR4302537.496645/1‑94 (MQ=60)

                           GCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTT                                                                    <  SRR4302537.3527465/94‑1 (MQ=60)

                           GCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTT                                                                    <  SRR4302537.2136536/94‑1 (MQ=60)

                            CGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTC                                                                   <  SRR4302537.4191634/94‑1 (MQ=60)

                             GTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCA                                                                  >  SRR4302537.4689205/1‑94 (MQ=60)

                             GTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCA                                                                  >  SRR4302537.5232852/1‑94 (MQ=60)

                              TTTGACCATCTTGACGATATGAACCAGGCCTTTTGGCAGCAAAATTTCGATCTGCCTCCGGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAG                                                                 <  SRR4302537.4517067/94‑1 (MQ=60)

                                TGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAA                                                               >  SRR4302537.1306465/1‑94 (MQ=60)

                                TGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAA                                                               <  SRR4302537.4902262/94‑1 (MQ=60)

                                TGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAA                                                               >  SRR4302537.1540024/1‑94 (MQ=60)

                                 GACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAG                                                              <  SRR4302537.5967295/94‑1 (MQ=60)

                                 GACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAG                                                              >  SRR4302537.3460207/1‑94 (MQ=60)

                                 GACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAG                                                              >  SRR4302537.1417762/1‑94 (MQ=60)

                                 GACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAG                                                              <  SRR4302537.1162214/94‑1 (MQ=60)

                                 GACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAG                                                              >  SRR4302537.4165457/1‑94 (MQ=60)

                                   CCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCG                                                            >  SRR4302537.1284007/1‑94 (MQ=60)

                                   CCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCG                                                            >  SRR4302537.3207268/1‑94 (MQ=60)

                                    CATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGT                                                           <  SRR4302537.5853736/94‑1 (MQ=60)

                                    CATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGT                                                           >  SRR4302537.569845/1‑94 (MQ=60)

                                     ATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTT                                                          <  SRR4302537.6472518/94‑1 (MQ=60)

                                     ATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTT                                                          <  SRR4302537.989934/94‑1 (MQ=60)

                                     ATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTT                                                          >  SRR4302537.1729065/1‑94 (MQ=60)

                                     ATCTTGACGATATGCACCAGGCCTTTTCGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTT                                                          <  SRR4302537.4114208/94‑1 (MQ=60)

                                      TCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTC                                                         <  SRR4302537.1702507/94‑1 (MQ=60)

                                      TCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTC                                                         <  SRR4302537.4647964/94‑1 (MQ=60)

                                      TCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTC                                                         >  SRR4302537.5742472/1‑94 (MQ=60)

                                       CTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTTG                                                        <  SRR4302537.6138760/94‑1 (MQ=60)

                                       CTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCG                                                        <  SRR4302537.2346433/94‑1 (MQ=60)

                                       CTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCG                                                        >  SRR4302537.2436737/1‑94 (MQ=60)

                                       CTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCG                                                        <  SRR4302537.521944/94‑1 (MQ=60)

                                       CTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCG                                                        >  SRR4302537.2572819/1‑94 (MQ=60)

                                        TTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGT                                                       >  SRR4302537.175398/1‑94 (MQ=60)

                                          GACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTAC                                                     >  SRR4302537.3543978/1‑94 (MQ=60)

                                           ACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACA                                                    >  SRR4302537.6469544/1‑94 (MQ=60)

                                           ACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACA                                                    >  SRR4302537.6469545/1‑94 (MQ=60)

                                           ACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACA                                                    <  SRR4302537.2187499/94‑1 (MQ=60)

                                            gaATATGCACAAGTCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATCCTTCAGAAGCGTTCGTACAA                                                   <  SRR4302537.5200704/92‑1 (MQ=60)

                                            CGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATCTCGTTAATTCTTCAGAAGCGTTCGTACAA                                                   >  SRR4302537.696618/1‑94 (MQ=60)

                                             GATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGGGTTCGTACAAC                                                  <  SRR4302537.5070627/94‑1 (MQ=60)

                                             GATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAAC                                                  >  SRR4302537.3303791/1‑94 (MQ=60)

                                              ATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACT                                                 <  SRR4302537.2771059/94‑1 (MQ=60)

                                              ATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACT                                                 >  SRR4302537.86785/1‑94 (MQ=60)

                                               TATGCACCAGGCCTTTTTGCAGCAAACCTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTT                                                <  SRR4302537.4029888/94‑1 (MQ=60)

                                               TATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTTCCCTGCCATATCGTTAATTCTTCAGAAGCCGTCGTACAACTT                                                >  SRR4302537.5779076/1‑94 (MQ=60)

                                               TATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTT                                                >  SRR4302537.5158793/1‑94 (MQ=60)

                                                  GCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCT                                             >  SRR4302537.4102249/1‑94 (MQ=60)

                                                   CACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTC                                            <  SRR4302537.5479561/94‑1 (MQ=60)

                                                   CACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTC                                            <  SRR4302537.2908009/94‑1 (MQ=60)

                                                   CACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTC                                            >  SRR4302537.2626365/1‑94 (MQ=60)

                                                    CCCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCG                                           <  SRR4302537.2594529/94‑1 (MQ=60)

                                                    ACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCG                                           >  SRR4302537.234793/1‑94 (MQ=60)

                                                    ACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCG                                           >  SRR4302537.4398320/1‑94 (MQ=60)

                                                     CCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGC                                          >  SRR4302537.1132594/1‑94 (MQ=60)

                                                     CCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGC                                          >  SRR4302537.5443290/1‑94 (MQ=60)

                                                     CCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAAATTGCTCGC                                          >  SRR4302537.6092740/1‑94 (MQ=60)

                                                      CAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCC                                         >  SRR4302537.2458036/1‑94 (MQ=60)

                                                       AGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCA                                        >  SRR4302537.869060/1‑94 (MQ=60)

                                                       AGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCA                                        <  SRR4302537.6159752/94‑1 (MQ=60)

                                                       AGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCA                                        <  SRR4302537.6360437/94‑1 (MQ=60)

                                                        GGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAG                                       <  SRR4302537.1377612/94‑1 (MQ=60)

                                                         GCCTTTTTGCAGCAAATCTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGG                                      <  SRR4302537.1594275/94‑1 (MQ=60)

                                                         GCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGG                                      >  SRR4302537.4503297/1‑94 (MQ=60)

                                                         GCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGG                                      >  SRR4302537.3068903/1‑94 (MQ=60)

                                                         GCCTTTTTCCAGCAAACCTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGG                                      <  SRR4302537.1226211/94‑1 (MQ=60)

                                                           CTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGC                                    >  SRR4302537.4571188/1‑94 (MQ=60)

                                                           CTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGC                                    >  SRR4302537.3825226/1‑94 (MQ=60)

                                                           CTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGC                                    >  SRR4302537.2752463/1‑94 (MQ=60)

                                                             TTTTGCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCAC                                  <  SRR4302537.190280/94‑1 (MQ=60)

                                                                 GCAGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACC                              <  SRR4302537.4934683/94‑1 (MQ=60)

                                                                   AGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACGACCTG                            >  SRR4302537.3839346/1‑94 (MQ=60)

                                                                   AGCAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTG                            >  SRR4302537.2049379/1‑94 (MQ=60)

                                                                     CAAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCT                          >  SRR4302537.1592227/1‑94 (MQ=60)

                                                                      AAAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTG                         >  SRR4302537.5573483/1‑94 (MQ=60)

                                                                       AAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGT                        >  SRR4302537.4677697/1‑94 (MQ=60)

                                                                       AAACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGT                        >  SRR4302537.900899/1‑94 (MQ=60)

                                                                        AACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTA                       >  SRR4302537.3883088/1‑94 (MQ=60)

                                                                        AACTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTA                       >  SRR4302537.978702/1‑94 (MQ=60)

                                                                          CTTCGATCTGCCTCCAGGGGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATG                     <  SRR4302537.1501208/94‑1 (MQ=60)

                                                                          CTTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATG                     <  SRR4302537.1187370/94‑1 (MQ=60)

                                                                          CTACGATCTGCCCCCAGGCGGCGTCCCCTGCCATATCGTTAATTCTTCAGAAGAGTTCGTACCACTTGCTCGCCAGGGCACCACCTGCTGTATG                     <  SRR4302537.2931095/94‑1 (MQ=60)

                                                                           TTCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGA                    <  SRR4302537.1552139/94‑1 (MQ=60)

                                                                            TCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGAT                   <  SRR4302537.5456020/94‑1 (MQ=60)

                                                                            TCGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGAT                   <  SRR4302537.6348316/94‑1 (MQ=60)

                                                                             CGATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATC                  <  SRR4302537.5562664/94‑1 (MQ=60)

                                                                             CGATCTGCCTCCAGGCGCCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATC                  <  SRR4302537.4072628/94‑1 (MQ=60)

                                                                              GATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCC                 >  SRR4302537.5492769/1‑94 (MQ=60)

                                                                              GATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCC                 >  SRR4302537.629721/1‑94 (MQ=60)

                                                                              GATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGGTCGTACAACTTGCTCGCCCGTGCACCACCTGCTGCATGATGC                 >  SRR4302537.2951046/1‑94 (MQ=60)

                                                                               ATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCC                >  SRR4302537.5404353/1‑94 (MQ=60)

                                                                               ATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCC                >  SRR4302537.1308385/1‑94 (MQ=60)

                                                                               ATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCC                >  SRR4302537.2475126/1‑94 (MQ=60)

                                                                               ATCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCC                >  SRR4302537.3735941/1‑94 (MQ=60)

                                                                                TCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCG               >  SRR4302537.5209105/1‑94 (MQ=60)

                                                                                TCTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAAGGCACCACCTGCTGTATGATCCCT               >  SRR4302537.4706665/1‑94 (MQ=60)

                                                                                 CTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGC              >  SRR4302537.3871783/1‑94 (MQ=60)

                                                                                 CTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGC              >  SRR4302537.1702358/1‑94 (MQ=60)

                                                                                 CTGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGC              >  SRR4302537.209738/1‑94 (MQ=60)

                                                                                  TGCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCA             >  SRR4302537.2495379/1‑94 (MQ=60)

                                                                                   GCCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCAC            >  SRR4302537.1550909/1‑94 (MQ=60)

                                                                                    CCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACC           >  SRR4302537.1025395/1‑94 (MQ=60)

                                                                                    CCTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGCATGATCCCGCACC           >  SRR4302537.6241944/1‑94 (MQ=60)

                                                                                     CTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCT          >  SRR4302537.5826606/1‑94 (MQ=60)

                                                                                     CTCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCT          >  SRR4302537.34784/1‑94 (MQ=60)

                                                                                      TCCGGGCGGCGTCCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTG         <  SRR4302537.563756/94‑1 (MQ=60)

                                                                                      TCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTG         <  SRR4302537.5995024/94‑1 (MQ=60)

                                                                                      CCCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTG         <  SRR4302537.3625507/94‑1 (MQ=60)

                                                                                       CCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGC        >  SRR4302537.4431650/1‑94 (MQ=60)

                                                                                       CCAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGC        >  SRR4302537.5808964/1‑94 (MQ=60)

                                                                                        CAGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCA       >  SRR4302537.2173267/1‑94 (MQ=60)

                                                                                         AGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATGCCGCACCCGCAA      >  SRR4302537.3281793/1‑94 (MQ=60)

                                                                                         AGGCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAA      <  SRR4302537.4732350/94‑1 (MQ=60)

                                                                                           GCGGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAAAT    >  SRR4302537.5414964/1‑94 (MQ=60)

                                                                                            CGGCGCGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCCCCTGCTGTATGATCCCGCACCTGCAAATC   <  SRR4302537.4724079/94‑1 (MQ=60)

                                                                                             GGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAAATCG  >  SRR4302537.2247773/1‑94 (MQ=60)

                                                                                             GGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAAATCG  >  SRR4302537.2279321/1‑94 (MQ=60)

TCGGCATTACTGAAAGCGCCAGTGGTCGCGTTTGACCATCTTGACGATATGCACCAGGCCTTTTTGCAGCAAAACTTCGATCTGCCTCCAGGCAGCGTGCCCTGCCATATCGTTAATTCTTCAGAAGCGTTCGTACAACTTGCTCGCCAGGGCACCACCTGCTGTATGATCCCGCACCTGCAAATCG  >  NC_000913/3058322‑3058508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`