Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,514	Δ1 bp	intergenic (‑165/+149)	cmtB ← / ← tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,513	0	C	.	100.0%	1025.6 / NA	216	intergenic (‑164/+150)	cmtB/tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (109/107); total (109/107)

Only 100 of 226 total aligned reads displayed.

GCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGC  >  NC_000913/3077421‑3077601

gCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAc                                                                                          <  2:812722/93‑1 (MQ=255)

gCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAc                                                                                          >  2:5982506/1‑93 (MQ=255)

 cAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcg                                                                                         <  2:2811909/93‑2 (MQ=255)

  aaTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgc                                                                                        >  1:3882009/1‑91 (MQ=255)

  aaTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgc                                                                                        <  1:31372/93‑3 (MQ=255)

   aTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgca                                                                                       >  1:922300/1‑90 (MQ=255)

    ttGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgcat                                                                                      >  2:3907236/1‑89 (MQ=255)

    ttGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAcgcat                                                                                      >  1:2606553/1‑89 (MQ=255)

        gCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCg                                                                                 <  1:4153658/93‑1 (MQ=255)

        gCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCg                                                                                 <  2:1102271/93‑1 (MQ=255)

        gCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCg                                                                                 >  1:6062049/1‑93 (MQ=255)

           gggAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGa                                                                              >  1:1283904/1‑93 (MQ=255)

           gggAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGa                                                                              <  1:3482352/93‑1 (MQ=255)

            ggAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGAt                                                                             >  2:2079103/1‑93 (MQ=255)

             gAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATg                                                                            >  2:1927370/1‑93 (MQ=255)

               aaCCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCa                                                                          >  1:5357180/1‑93 (MQ=255)

                 ccGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCata                                                                        >  2:3582141/1‑93 (MQ=255)

                  cGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCatat                                                                       <  1:1858275/93‑1 (MQ=255)

                   ggTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATAtt                                                                      >  1:2598660/1‑93 (MQ=255)

                     tgtTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTAc                                                                    >  1:2789641/1‑93 (MQ=255)

                     tgtTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTAc                                                                    >  1:2920584/1‑93 (MQ=255)

                      gtTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACa                                                                   >  1:2259919/1‑93 (MQ=255)

                      gtTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACa                                                                   >  1:1506641/1‑93 (MQ=255)

                       ttttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAt                                                                  >  2:3759071/1‑93 (MQ=255)

                       ttttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAt                                                                  <  2:4991661/93‑1 (MQ=255)

                       ttttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAt                                                                  >  2:479547/1‑93 (MQ=255)

                       ttttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAt                                                                  >  2:1565865/1‑93 (MQ=255)

                        tttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATa                                                                 <  2:2172667/93‑1 (MQ=255)

                        tttGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATa                                                                 <  2:2770179/93‑1 (MQ=255)

                          tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               <  2:4334406/93‑1 (MQ=255)

                          tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               <  2:4502910/93‑1 (MQ=255)

                          tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               <  2:3122450/93‑1 (MQ=255)

                          tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               >  2:1271657/1‑93 (MQ=255)

                          tGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAt                                                               <  1:3565008/93‑1 (MQ=255)

                           gAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAtt                                                              >  1:935068/1‑93 (MQ=255)

                           gAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAAtt                                                              <  1:4400864/93‑1 (MQ=255)

                            aaaaCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTg                                                             >  2:3223324/1‑93 (MQ=255)

                            aaaaCAGTGAATTATTAATCTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTg                                                             >  2:6278101/1‑93 (MQ=255)

                             aaaCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGa                                                            <  2:5641625/93‑1 (MQ=255)

                              aaCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGat                                                           <  1:2390810/93‑1 (MQ=255)

                               aCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGata                                                          <  1:5125461/93‑1 (MQ=255)

                                cAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGatat                                                         <  2:1625545/93‑1 (MQ=255)

                                cAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGatat                                                         >  1:4890539/1‑93 (MQ=255)

                                 aGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatt                                                        <  2:536001/93‑1 (MQ=255)

                                  gTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtattt                                                       >  1:6427161/1‑93 (MQ=255)

                                  gTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtattt                                                       >  2:4598618/1‑93 (MQ=255)

                                   tGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtattta                                                      >  2:6140239/1‑93 (MQ=255)

                                    gAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttat                                                     >  2:3192191/1‑93 (MQ=255)

                                     aaTTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAtatttatt                                                    <  2:4217598/93‑1 (MQ=255)

                                      attattAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTg                                                   >  2:3853142/1‑93 (MQ=255)

                                       ttattaATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGc                                                  >  1:1472801/1‑93 (MQ=255)

                                        tattaATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCa                                                 >  2:2615779/1‑93 (MQ=255)

                                         attaATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCat                                                >  1:2203753/1‑93 (MQ=255)

                                          ttaATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCata                                               >  2:6040559/1‑93 (MQ=255)

                                            aaTTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCAtata                                             <  2:1034734/93‑1 (MQ=255)

                                              tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           <  2:852669/93‑1 (MQ=255)

                                              tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           >  2:1670474/1‑93 (MQ=255)

                                              tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           >  1:4114168/1‑93 (MQ=255)

                                              tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           <  1:5082380/93‑1 (MQ=255)

                                              tttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATaaa                                           >  1:2136520/1‑93 (MQ=255)

                                               ttCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAat                                          >  1:6130129/1‑93 (MQ=255)

                                                tCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAata                                         >  2:3731985/1‑93 (MQ=255)

                                                 cTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAatat                                        >  1:1711912/1‑93 (MQ=255)

                                                 cTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAatat                                        >  1:5756792/1‑93 (MQ=255)

                                                    atataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtg                                     <  1:744968/93‑1 (MQ=255)

                                                     tataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgt                                    >  1:5779757/1‑93 (MQ=255)

                                                     tataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgt                                    <  1:5413705/93‑1 (MQ=255)

                                                     tataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgt                                    >  1:5630771/1‑93 (MQ=255)

                                                      ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   >  1:5472470/1‑93 (MQ=255)

                                                      ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   <  1:5044874/93‑1 (MQ=255)

                                                      ataACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtgtg                                   >  1:6012794/1‑93 (MQ=255)

                                                       taACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGa                                  >  1:1970011/1‑93 (MQ=255)

                                                        aaCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAt                                 <  2:5964539/93‑1 (MQ=255)

                                                        aaCATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAt                                 <  2:1386913/93‑1 (MQ=255)

                                                           aTTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTa                              >  1:3331605/1‑93 (MQ=255)

                                                              gAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaa                           <  1:5445607/93‑1 (MQ=255)

                                                              gAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaa                           <  1:3409766/93‑1 (MQ=255)

                                                               aGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaac                          >  2:5793106/1‑93 (MQ=255)

                                                               aGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTacaac                          <  1:3683354/93‑1 (MQ=255)

                                                                 ttATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAacac                        >  2:3016822/1‑93 (MQ=255)

                                                                 ttATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAacac                        >  2:1845163/1‑93 (MQ=255)

                                                                   ataGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGc                      <  1:4534100/93‑1 (MQ=255)

                                                                     aGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCt                    <  1:4246054/93‑1 (MQ=255)

                                                                      gATATAACCACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCtt                   >  2:6322909/1‑93 (MQ=255)

                                                                      gATATAAAAAAAAAAGTCAATA‑AGCATATAGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTTTGATCTACAACACGCCtt                   >  2:4100797/1‑93 (MQ=255)

                                                                       atatAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTa                  >  2:4055822/1‑93 (MQ=255)

                                                                         ataACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATc                >  2:537732/1‑93 (MQ=255)

                                                                          taACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCt               >  2:243183/1‑93 (MQ=255)

                                                                           aacaacAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTa              <  1:824428/93‑1 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  2:4675199/1‑93 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  2:5193927/1‑93 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             <  2:736760/93‑1 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  1:5706322/1‑93 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             >  1:181479/1‑93 (MQ=255)

                                                                            acaacaAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAt             <  1:1797711/93‑1 (MQ=255)

                                                                               acaaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGc          >  2:4854615/1‑93 (MQ=255)

                                                                                caaATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCt         <  1:3925022/93‑1 (MQ=255)

                                                                                 aaaTGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCtt        >  1:4932535/1‑93 (MQ=255)

                                                                                       cAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGc  >  2:4564893/1‑93 (MQ=255)

                                                                                       cAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGc  <  1:1367591/93‑1 (MQ=255)

GCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGC  >  NC_000913/3077421‑3077601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACG  >  NC_000913/3077423‑3077606

AATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCA                                                                                           >  SRR4302537.928667/1‑94 (MQ=60)

AATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCA                                                                                           <  SRR4302537.31624/94‑1 (MQ=60)

 ATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCAT                                                                                          >  SRR4302537.2624776/1‑94 (MQ=60)

   TGAGCAGGGAAACCGGTGTTTTGAAAACAGTAAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATAT                                                                                        <  SRR4302537.3468328/94‑1 (MQ=60)

    GAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATC                                                                                       <  SRR4302537.6075142/94‑1 (MQ=60)

    GAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATC                                                                                       <  SRR4302537.5884943/94‑1 (MQ=60)

    GAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATC                                                                                       <  SRR4302537.4195230/94‑1 (MQ=60)

     AGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCG                                                                                      >  SRR4302537.6103700/1‑94 (MQ=60)

     AGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCG                                                                                      <  SRR4302537.5621101/94‑1 (MQ=60)

      GCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGT                                                                                     <  SRR4302537.1976177/94‑1 (MQ=60)

      GCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGT                                                                                     <  SRR4302537.4182660/94‑1 (MQ=60)

        AGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGA                                                                                   >  SRR4302537.1292832/1‑94 (MQ=60)

         GGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGAT                                                                                  <  SRR4302537.3506991/94‑1 (MQ=60)

            AAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCA                                                                               >  SRR4302537.5393871/1‑94 (MQ=60)

            AAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCA                                                                               <  SRR4302537.5438632/94‑1 (MQ=60)

            AAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCA                                                                               >  SRR4302537.2299510/1‑94 (MQ=60)

              ACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATA                                                                             <  SRR4302537.675410/94‑1 (MQ=60)

               CCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATAT                                                                            <  SRR4302537.1688663/94‑1 (MQ=60)

                CGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATT                                                                           >  SRR4302537.4332857/1‑94 (MQ=60)

                CGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATT                                                                           <  SRR4302537.1871219/94‑1 (MQ=60)

                CGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATT                                                                           >  SRR4302537.2616838/1‑94 (MQ=60)

                  GTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTAC                                                                         <  SRR4302537.5313584/94‑1 (MQ=60)

                  GTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTAC                                                                         >  SRR4302537.2941109/1‑94 (MQ=60)

                  GTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTAC                                                                         >  SRR4302537.2809140/1‑94 (MQ=60)

                   TGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACA                                                                        >  SRR4302537.1517151/1‑94 (MQ=60)

                   TGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACA                                                                        >  SRR4302537.2704142/1‑94 (MQ=60)

                   TGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACA                                                                        >  SRR4302537.2275867/1‑94 (MQ=60)

                    GTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACAT                                                                       >  SRR4302537.1298335/1‑94 (MQ=60)

                     TTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATA                                                                      <  SRR4302537.237216/94‑1 (MQ=60)

                     TTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATA                                                                      <  SRR4302537.3920250/94‑1 (MQ=60)

                        TGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATT                                                                   <  SRR4302537.3590152/94‑1 (MQ=60)

                        TGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATT                                                                   >  SRR4302537.941514/1‑94 (MQ=60)

                         GAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTG                                                                  <  SRR4302537.4431234/94‑1 (MQ=60)

                          AAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGA                                                                 >  SRR4302537.1815239/1‑94 (MQ=60)

                           AAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAT                                                                <  SRR4302537.930587/94‑1 (MQ=60)

                           AAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGAT                                                                <  SRR4302537.3356700/94‑1 (MQ=60)

                            AACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATA                                                               <  SRR4302537.4508338/94‑1 (MQ=60)

                            AACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATA                                                               <  SRR4302537.2407645/94‑1 (MQ=60)

                            AACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATA                                                               <  SRR4302537.5286303/94‑1 (MQ=60)

                             ACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATAT                                                              >  SRR4302537.4924308/1‑94 (MQ=60)

                             ACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATAT                                                              <  SRR4302537.5160783/94‑1 (MQ=60)

                             ACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATAT                                                              <  SRR4302537.2014697/94‑1 (MQ=60)

                              CAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATT                                                             >  SRR4302537.939832/1‑94 (MQ=60)

                              CAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATAGTGATGCATATTACATAATTGATATT                                                             <  SRR4302537.3288200/94‑1 (MQ=60)

                               AGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTT                                                            >  SRR4302537.6470835/1‑94 (MQ=60)

                                   AATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTG                                                        <  SRR4302537.4939080/94‑1 (MQ=60)

                                   AATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTG                                                        >  SRR4302537.2506605/1‑94 (MQ=60)

                                    ATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGC                                                       >  SRR4302537.5505355/1‑94 (MQ=60)

                                    ATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGC                                                       >  SRR4302537.1483037/1‑94 (MQ=60)

                                    ATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGC                                                       >  SRR4302537.5145248/1‑94 (MQ=60)

                                     TTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCA                                                      <  SRR4302537.186059/94‑1 (MQ=60)

                                      TATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCAT                                                     >  SRR4302537.2219295/1‑94 (MQ=60)

                                       ATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATA                                                    <  SRR4302537.2224979/94‑1 (MQ=60)

                                          AATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAA                                                 <  SRR4302537.3544196/94‑1 (MQ=60)

                                          AATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAA                                                 >  SRR4302537.6479695/1‑94 (MQ=60)

                                           ATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAA                                                >  SRR4302537.2151563/1‑94 (MQ=60)

                                           ATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAA                                                >  SRR4302537.4142908/1‑94 (MQ=60)

                                            TTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAAT                                               <  SRR4302537.6166772/94‑1 (MQ=60)

                                            TTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAAT                                               >  SRR4302537.6172266/1‑94 (MQ=60)

                                            TTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAAT                                               <  SRR4302537.5117362/94‑1 (MQ=60)

                                              TCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAT                                             >  SRR4302537.5796222/1‑94 (MQ=60)

                                              TCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAT                                             >  SRR4302537.1723868/1‑94 (MQ=60)

                                              TCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAT                                             >  SRR4302537.955349/1‑94 (MQ=60)

                                                TTATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTT                                           >  SRR4302537.1926571/1‑94 (MQ=60)

                                                  ATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGT                                         >  SRR4302537.804118/1‑94 (MQ=60)

                                                  ATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGT                                         <  SRR4302537.750046/94‑1 (MQ=60)

                                                  ATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGT                                         >  SRR4302537.5669295/1‑94 (MQ=60)

                                                  ATATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGT                                         >  SRR4302537.5819369/1‑94 (MQ=60)

                                                   TATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTG                                        >  SRR4302537.5509813/1‑94 (MQ=60)

                                                   TATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTG                                        >  SRR4302537.4253962/1‑94 (MQ=60)

                                                   TATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTG                                        <  SRR4302537.5450616/94‑1 (MQ=60)

                                                   TATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTG                                        >  SRR4302537.6054105/1‑94 (MQ=60)

                                                   CATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTG                                        <  SRR4302537.6247460/94‑1 (MQ=60)

                                                    ATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGA                                       >  SRR4302537.1983711/1‑94 (MQ=60)

                                                    ATAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGA                                       <  SRR4302537.5079618/94‑1 (MQ=60)

                                                     TAACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAT                                      <  SRR4302537.5109970/94‑1 (MQ=60)

                                                      AACATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATC                                     >  SRR4302537.1781007/1‑94 (MQ=60)

                                                        CATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTA                                   >  SRR4302537.3955598/1‑94 (MQ=60)

                                                        CATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTA                                   >  SRR4302537.577932/1‑94 (MQ=60)

                                                        CATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTA                                   <  SRR4302537.5724744/94‑1 (MQ=60)

                                                        CATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTA                                   >  SRR4302537.3355177/1‑94 (MQ=60)

                                                         ATTGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTAC                                  >  SRR4302537.2924502/1‑94 (MQ=60)

                                                           TGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAA                                >  SRR4302537.589888/1‑94 (MQ=60)

                                                           TGAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAA                                <  SRR4302537.1415786/94‑1 (MQ=60)

                                                            GAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAAC                               <  SRR4302537.3433919/94‑1 (MQ=60)

                                                            GAGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAAC                               <  SRR4302537.5482755/94‑1 (MQ=60)

                                                             AGTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACA                              <  SRR4302537.3709257/94‑1 (MQ=60)

                                                              GTTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACAC                             >  SRR4302537.2619629/1‑94 (MQ=60)

                                                               TTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATCTTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACC                            >  SRR4302537.4936230/1‑94 (MQ=60)

                                                               TTATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACG                            <  SRR4302537.2932432/94‑1 (MQ=60)

                                                                 ATAGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCC                          <  SRR4302537.4565492/94‑1 (MQ=60)

                                                                   AGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTT                        >  SRR4302537.3591292/1‑94 (MQ=60)

                                                                   AGATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTT                        <  SRR4302537.4275562/94‑1 (MQ=60)

                                                                    GATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTA                       >  SRR4302537.4164151/1‑94 (MQ=60)

                                                                    GATATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTA                       >  SRR4302537.3095003/1‑94 (MQ=60)

                                                                       ATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCT                    <  SRR4302537.3814121/94‑1 (MQ=60)

                                                                       ATAACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCT                    >  SRR4302537.2197796/1‑94 (MQ=60)

                                                                         AACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAT                  >  SRR4302537.182767/1‑94 (MQ=60)

                                                                         AACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAT                  >  SRR4302537.5535940/1‑94 (MQ=60)

                                                                         AACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAT                  <  SRR4302537.830081/94‑1 (MQ=60)

                                                                         AACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAT                  >  SRR4302537.5745373/1‑94 (MQ=60)

                                                                         AACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTAT                  <  SRR4302537.1500287/94‑1 (MQ=60)

                                                                          ACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATT                 >  SRR4302537.5801984/1‑94 (MQ=60)

                                                                          ACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATT                 >  SRR4302537.833487/1‑94 (MQ=60)

                                                                          ACAACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATT                 <  SRR4302537.1810261/94‑1 (MQ=60)

                                                                            AACAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGC               >  SRR4302537.4865474/1‑94 (MQ=60)

                                                                              CAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTT             >  SRR4302537.4966533/1‑94 (MQ=60)

                                                                              CAAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTT             <  SRR4302537.3952443/94‑1 (MQ=60)

                                                                               AAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTTTTGCTTG            >  SRR4302537.3471569/1‑94 (MQ=60)

                                                                               AAATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTG            <  SRR4302537.5045492/94‑1 (MQ=60)

                                                                                 ATGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTC          >  SRR4302537.3913498/1‑94 (MQ=60)

                                                                                  TGTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCC         <  SRR4302537.1603244/94‑1 (MQ=60)

                                                                                   GTCAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCG        <  SRR4302537.5454392/94‑1 (MQ=60)

                                                                                     CAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCA      >  SRR4302537.4623654/1‑94 (MQ=60)

                                                                                     CAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCA      <  SRR4302537.1377122/94‑1 (MQ=60)

                                                                                     CAATA‑CGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCA      >  SRR4302537.4884565/1‑94 (MQ=60)

                                                                                         taCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAC   <  SRR4302537.798952/92‑1 (MQ=60)

                                                                                         taCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAC   <  SRR4302537.6291640/92‑1 (MQ=60)

                                                                                          ACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACG  <  SRR4302537.2276254/94‑1 (MQ=60)

AATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACG  >  NC_000913/3077423‑3077606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`