Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,078,245 C→G G470G (GGG→GGC)  tktA ← transketolase 1 isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,078,2450CG99.2% 781.8 / ‑5.0 236G470G (GGG→GGC) tktAtransketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (129/104);  minor base T (0/2);  total (129/107)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 250 total aligned reads displayed.

ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078153‑3078334
                                                                                            |                                                                                         
aCCGCGGATTCAACCTGGCCACACGGAGGCCATGAAAACATGTTCGGGGTTAGGGGCAAAGAGGGGACTGGCTCACCCGGCGGGGGAGCCGGc                                                                                           >  2:6048236/1‑93 (MQ=255)
  cgcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                         >  1:789258/1‑93 (MQ=255)
   gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAAGCGGCTGGTGAGGCGGGCCg                                                                                        >  1:30735/1‑93 (MQ=255)
   gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                                        >  2:4630219/1‑93 (MQ=255)
     ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                                      >  1:2517785/1‑93 (MQ=255)
      gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                     >  1:3274825/1‑93 (MQ=255)
       aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                                    <  2:3754118/93‑1 (MQ=255)
        ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   >  2:714743/1‑93 (MQ=255)
        ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                   <  1:2674682/93‑1 (MQ=255)
         tCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg                                                                                  >  2:1796094/1‑93 (MQ=255)
           aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                >  1:4247018/1‑93 (MQ=255)
           aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                <  1:2865282/93‑1 (MQ=255)
           aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                                                >  2:5161014/1‑93 (MQ=255)
            aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                                               >  1:5086944/1‑93 (MQ=255)
             ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                                              <  2:149274/93‑1 (MQ=255)
               tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCTAGa                                                                            >  1:5353008/1‑93 (MQ=255)
               tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                            <  2:4811802/93‑1 (MQ=255)
                ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                                           <  1:1930478/93‑1 (MQ=255)
                 gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                          <  2:4749711/93‑1 (MQ=255)
                 gTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                          >  2:5257475/1‑93 (MQ=255)
                   cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        >  1:3189910/1‑93 (MQ=255)
                   cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                                        >  2:1007570/1‑93 (MQ=255)
                     cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      >  1:4583296/1‑93 (MQ=255)
                     cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                      <  2:3680059/93‑1 (MQ=255)
                         gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                  >  2:241044/1‑93 (MQ=255)
                         gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                  <  1:1089700/93‑1 (MQ=255)
                          aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                 <  2:706775/93‑1 (MQ=255)
                           cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                >  1:2955342/1‑93 (MQ=255)
                            gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                               <  1:4126328/93‑1 (MQ=255)
                             ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                              <  1:2369285/93‑1 (MQ=255)
                              cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             >  2:5342883/1‑93 (MQ=255)
                              cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                             <  2:1983291/93‑1 (MQ=255)
                               aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  2:5679225/1‑93 (MQ=255)
                               aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            <  1:5925614/93‑1 (MQ=255)
                               aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  1:3031406/1‑93 (MQ=255)
                               aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                            >  1:520565/1‑93 (MQ=255)
                                tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  2:1684936/1‑93 (MQ=255)
                                tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  1:911305/1‑93 (MQ=255)
                                tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                           >  1:5863191/1‑93 (MQ=255)
                                  tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                                         >  1:2104017/1‑93 (MQ=255)
                                    gACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                       >  1:1507083/1‑93 (MQ=255)
                                      cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                                     >  1:4038435/1‑93 (MQ=255)
                                       aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    >  2:4916242/1‑93 (MQ=255)
                                       aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                    <  1:3480665/93‑1 (MQ=255)
                                        tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  1:5429056/1‑93 (MQ=255)
                                        tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                   >  2:2230350/1‑93 (MQ=255)
                                         ggtCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                                                  <  1:2861569/91‑1 (MQ=255)
                                         gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                                                  <  2:5808564/93‑1 (MQ=255)
                                          ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGGAAACCATc                                                 >  2:2710169/1‑93 (MQ=255)
                                             ggggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                                              <  1:5480478/93‑1 (MQ=255)
                                              gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                             <  1:4190388/93‑1 (MQ=255)
                                                gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                           >  1:4456185/1‑93 (MQ=255)
                                                 ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  1:1292524/93‑1 (MQ=255)
                                                 ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                          <  2:4244137/93‑1 (MQ=255)
                                                     gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                                      >  1:6099960/1‑93 (MQ=255)
                                                      cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                     <  2:2840768/93‑1 (MQ=255)
                                                       gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                    >  1:1981898/1‑93 (MQ=255)
                                                        cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                                   <  1:372133/93‑1 (MQ=255)
                                                          gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 >  2:4142584/1‑93 (MQ=255)
                                                          gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                 >  2:839707/1‑93 (MQ=255)
                                                          gagaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCGGGTtca                                 >  2:5720283/1‑93 (MQ=255)
                                                            gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:6248902/1‑93 (MQ=255)
                                                            gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc                               >  2:2375729/1‑93 (MQ=255)
                                                             aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca                              <  2:3365020/93‑1 (MQ=255)
                                                              aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                             <  2:907147/93‑1 (MQ=255)
                                                              aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                             <  1:2916431/93‑1 (MQ=255)
                                                               gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                            <  1:159529/93‑1 (MQ=255)
                                                               gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                            >  1:3548267/1‑93 (MQ=255)
                                                               gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                            <  1:741174/93‑1 (MQ=255)
                                                                cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcg                           >  1:2763041/1‑93 (MQ=255)
                                                                 gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGGGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          <  2:2922724/93‑1 (MQ=255)
                                                                 gACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                          <  2:5949389/93‑1 (MQ=255)
                                                                  aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGATTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         <  1:4549370/93‑1 (MQ=255)
                                                                  aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                         >  1:2301425/1‑93 (MQ=255)
                                                                    cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc                       >  1:6006448/1‑93 (MQ=255)
                                                                      gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCa                     <  1:5549502/93‑1 (MQ=255)
                                                                       cTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAt                    >  1:4495573/1‑93 (MQ=255)
                                                                        tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   >  2:6336206/1‑93 (MQ=255)
                                                                        tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   <  2:648573/93‑1 (MQ=255)
                                                                        tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                   >  2:1416682/1‑93 (MQ=255)
                                                                         cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  <  1:4955768/93‑1 (MQ=255)
                                                                         cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  <  1:4849830/93‑1 (MQ=255)
                                                                         cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  >  1:6392323/1‑93 (MQ=255)
                                                                         cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                  >  2:3280859/1‑93 (MQ=255)
                                                                          aaCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacg                 >  2:3549252/1‑93 (MQ=255)
                                                                           aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgt                >  1:6268204/1‑93 (MQ=255)
                                                                            ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgta               <  2:2017744/93‑1 (MQ=255)
                                                                            ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgta               <  1:2218966/93‑1 (MQ=255)
                                                                             cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              >  1:1694673/1‑93 (MQ=255)
                                                                             cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              <  2:4499299/93‑1 (MQ=255)
                                                                             cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac              >  1:3924916/1‑93 (MQ=255)
                                                                              ggCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg             >  2:3154640/1‑93 (MQ=255)
                                                                               gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg            >  1:1780579/1‑93 (MQ=255)
                                                                                cTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGc           >  1:218593/1‑93 (MQ=255)
                                                                                  ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGt         >  2:1352180/1‑93 (MQ=255)
                                                                                     gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTAc      <  2:3072174/93‑1 (MQ=255)
                                                                                      aGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACg     <  2:5066106/93‑1 (MQ=255)
                                                                                       gTCGGGCCGTCTCCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGt    <  2:2467448/93‑1 (MQ=255)
                                                                                       gTCGGCCCGTCTTCGCCCAGACGGATGGAGTCGTGGGTGTAAACCATCGCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGt    <  2:1189647/93‑1 (MQ=255)
                                                                                         cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc  >  2:4738837/1‑93 (MQ=255)
                                                                                            |                                                                                         
ACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGC  >  NC_000913/3078153‑3078334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  >  NC_000913/3078154‑3078336
                                                                                           |                                                                                           
CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCC                                                                                           >  SRR4302537.6097522/1‑94 (MQ=60)
CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCC                                                                                           >  SRR4302537.794678/1‑94 (MQ=60)
 CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAAGCGGCTGGTGAGGCGGGCCG                                                                                          >  SRR4302537.30982/1‑94 (MQ=60)
 CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCG                                                                                          <  SRR4302537.5377544/94‑1 (MQ=60)
 CGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCG                                                                                          >  SRR4302537.3836960/1‑94 (MQ=60)
   CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                                        >  SRR4302537.3588119/1‑94 (MQ=60)
   CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                                        >  SRR4302537.6443815/1‑94 (MQ=60)
   CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                                        >  SRR4302537.2535475/1‑94 (MQ=60)
    GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       <  SRR4302537.3129583/94‑1 (MQ=60)
    GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       >  SRR4302537.3297934/1‑94 (MQ=60)
    GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       >  SRR4302537.2983331/1‑94 (MQ=60)
    GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                                       >  SRR4302537.2869787/1‑94 (MQ=60)
     GATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTT                                                                                      >  SRR4302537.2699197/1‑94 (MQ=60)
      ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                                                     >  SRR4302537.4830738/1‑94 (MQ=60)
       TTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCG                                                                                    <  SRR4302537.2693361/94‑1 (MQ=60)
         CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCC                                                                                  >  SRR4302537.4276534/1‑94 (MQ=60)
         CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCC                                                                                  >  SRR4302537.915826/1‑94 (MQ=60)
         CAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCC                                                                                  >  SRR4302537.5534561/1‑94 (MQ=60)
          AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                 <  SRR4302537.2885372/94‑1 (MQ=60)
          AACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCC                                                                                 >  SRR4302537.5121966/1‑94 (MQ=60)
           ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                                                >  SRR4302537.3999179/1‑94 (MQ=60)
             CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCTAGA                                                                              >  SRR4302537.5389671/1‑94 (MQ=60)
               GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                                            <  SRR4302537.1943908/94‑1 (MQ=60)
                 TCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGA                                                                          >  SRR4302537.3212344/1‑94 (MQ=60)
                  CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                                         <  SRR4302537.5311855/94‑1 (MQ=60)
                   ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                                        >  SRR4302537.4614997/1‑94 (MQ=60)
                     CCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGA                                                                      <  SRR4302537.5126855/94‑1 (MQ=60)
                      CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAG                                                                     >  SRR4302537.4506853/1‑94 (MQ=60)
                        GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                                                   <  SRR4302537.1097226/94‑1 (MQ=60)
                         ACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCG                                                                  >  SRR4302537.2976080/1‑94 (MQ=60)
                         ACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCG                                                                  >  SRR4302537.4179345/1‑94 (MQ=60)
                          CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGG                                                                 >  SRR4302537.2471807/1‑94 (MQ=60)
                          CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGG                                                                 >  SRR4302537.5629746/1‑94 (MQ=60)
                          CGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGCGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGT                                                                 >  SRR4302537.831597/1‑94 (MQ=60)
                           GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                >  SRR4302537.2185769/1‑94 (MQ=60)
                           GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                <  SRR4302537.4155129/94‑1 (MQ=60)
                           GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                                                <  SRR4302537.509154/94‑1 (MQ=60)
                            CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302537.3213418/94‑1 (MQ=60)
                            CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302537.1876106/94‑1 (MQ=60)
                            CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                                               <  SRR4302537.2385984/94‑1 (MQ=60)
                             CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                              >  SRR4302537.524147/1‑94 (MQ=60)
                             CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                                              >  SRR4302537.3052541/1‑94 (MQ=60)
                              ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                             <  SRR4302537.5966279/94‑1 (MQ=60)
                              ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                             <  SRR4302537.3443386/94‑1 (MQ=60)
                              ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                             >  SRR4302537.917593/1‑94 (MQ=60)
                              ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                                             >  SRR4302537.5903403/1‑94 (MQ=60)
                               TGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAGCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTG                                                            <  SRR4302537.6367620/94‑1 (MQ=60)
                                GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                           <  SRR4302537.1991275/94‑1 (MQ=60)
                                GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                           >  SRR4302537.2118848/1‑94 (MQ=60)
                                GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAAACGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                                           >  SRR4302537.4572026/1‑94 (MQ=60)
                                 TAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTA                                                          >  SRR4302537.5511005/1‑94 (MQ=60)
                                  AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                         >  SRR4302537.2233174/1‑94 (MQ=60)
                                  AGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                                         >  SRR4302537.1517599/1‑94 (MQ=60)
                                   GACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAA                                                        <  SRR4302537.258628/94‑1 (MQ=60)
                                    ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       <  SRR4302537.2888369/94‑1 (MQ=60)
                                    ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       >  SRR4302537.4066692/1‑94 (MQ=60)
                                    ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       >  SRR4302537.6170571/1‑94 (MQ=60)
                                    ACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                                       >  SRR4302537.5495070/1‑94 (MQ=60)
                                     CATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                                                      >  SRR4302537.3498475/1‑94 (MQ=60)
                                      ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                     >  SRR4302537.5466076/1‑94 (MQ=60)
                                      ATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                                                     <  SRR4302537.3505289/94‑1 (MQ=60)
                                        GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                                                   <  SRR4302537.2881635/94‑1 (MQ=60)
                                        GTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTAGTCCAGACCGATGGAGTCGTGGGTGGAAACCATC                                                   >  SRR4302537.6294207/1‑94 (MQ=60)
                                        GGTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                                                   <  SRR4302537.2881634/94‑1 (MQ=60)
                                          TCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAC                                                 <  SRR4302537.1307412/94‑1 (MQ=60)
                                           CGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                                                >  SRR4302537.2919764/1‑94 (MQ=60)
                                            GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                               <  SRR4302537.1835665/94‑1 (MQ=60)
                                            GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                               <  SRR4302537.5899599/94‑1 (MQ=60)
                                            GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                               <  SRR4302537.705229/94‑1 (MQ=60)
                                            GGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                                               <  SRR4302537.5517893/94‑1 (MQ=60)
                                             GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                                              <  SRR4302537.1704392/94‑1 (MQ=60)
                                             GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                                              <  SRR4302537.3539111/94‑1 (MQ=60)
                                             GGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                                              <  SRR4302537.4219657/94‑1 (MQ=60)
                                              GGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                                             >  SRR4302537.4486958/1‑94 (MQ=60)
                                                TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGTCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                           <  SRR4302537.1301513/94‑1 (MQ=60)
                                                TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                                           >  SRR4302537.2820958/1‑94 (MQ=60)
                                                TTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCCTGGGTGTAAACCATCACCTGACG                                           >  SRR4302537.2943703/1‑94 (MQ=60)
                                                   CGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTG                                        >  SRR4302537.6141874/1‑94 (MQ=60)
                                                   CGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTG                                        <  SRR4302537.4226822/94‑1 (MQ=60)
                                                   CGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTG                                        >  SRR4302537.4857521/1‑94 (MQ=60)
                                                    GCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGT                                       <  SRR4302537.5020567/94‑1 (MQ=60)
                                                     CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                      >  SRR4302537.1995669/1‑94 (MQ=60)
                                                     CGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT                                      <  SRR4302537.3649301/94‑1 (MQ=60)
                                                       CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    >  SRR4302537.222875/1‑94 (MQ=60)
                                                       CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    >  SRR4302537.1307658/1‑94 (MQ=60)
                                                       CAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC                                    <  SRR4302537.374715/94‑1 (MQ=60)
                                                        AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                   >  SRR4302537.4045445/1‑94 (MQ=60)
                                                        AGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA                                   >  SRR4302537.1625031/1‑94 (MQ=60)
                                                          AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                 <  SRR4302537.2374796/94‑1 (MQ=60)
                                                          AGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATC                                 <  SRR4302537.2322985/94‑1 (MQ=60)
                                                             AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                              <  SRR4302537.2936918/94‑1 (MQ=60)
                                                             AGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC                              >  SRR4302537.3573334/1‑94 (MQ=60)
                                                              GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                             >  SRR4302537.2782318/1‑94 (MQ=60)
                                                              GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                             <  SRR4302537.5437210/94‑1 (MQ=60)
                                                              GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                             <  SRR4302537.160663/94‑1 (MQ=60)
                                                              GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                             <  SRR4302537.4469534/94‑1 (MQ=60)
                                                              GCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCG                             <  SRR4302537.746236/94‑1 (MQ=60)
                                                               CGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGA                            >  SRR4302537.794366/1‑94 (MQ=60)
                                                                GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           >  SRR4302537.268188/1‑94 (MQ=60)
                                                                GACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA                           >  SRR4302537.2317671/1‑94 (MQ=60)
                                                                 ACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGATTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG                          <  SRR4302537.4580855/94‑1 (MQ=60)
                                                                  CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCCGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                         >  SRR4302537.320222/1‑94 (MQ=60)
                                                                  CCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC                         >  SRR4302537.6047712/1‑94 (MQ=60)
                                                                   CTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCC                        <  SRR4302537.2294371/94‑1 (MQ=60)
                                                                    TGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCA                       <  SRR4302537.1999225/94‑1 (MQ=60)
                                                                     GCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAT                      >  SRR4302537.4526672/1‑94 (MQ=60)
                                                                     GCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAT                      <  SRR4302537.5587396/94‑1 (MQ=60)
                                                                     GCCCACCCGGCTGGCGAATCGGCCCATCTTCGCCCAGACCGATGGAGTCGTGGGTTTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAT                      <  SRR4302537.3971775/94‑1 (MQ=60)
                                                                       TCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATAC                    >  SRR4302537.6435869/1‑94 (MQ=60)
                                                                        CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   <  SRR4302537.4989938/94‑1 (MQ=60)
                                                                        CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   <  SRR4302537.4883296/94‑1 (MQ=60)
                                                                        CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   >  SRR4302537.4965629/1‑94 (MQ=60)
                                                                        CAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACG                   <  SRR4302537.4600464/94‑1 (MQ=60)
                                                                         AACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGT                  >  SRR4302537.6311226/1‑94 (MQ=60)
                                                                         AACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGT                  >  SRR4302537.6025614/1‑94 (MQ=60)
                                                                         AACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGT                  <  SRR4302537.929616/94‑1 (MQ=60)
                                                                           CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302537.4566329/94‑1 (MQ=60)
                                                                           CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                >  SRR4302537.3952336/1‑94 (MQ=60)
                                                                           CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302537.3068793/94‑1 (MQ=60)
                                                                           CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                >  SRR4302537.1706531/1‑94 (MQ=60)
                                                                           CCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTAC                <  SRR4302537.2234604/94‑1 (MQ=60)
                                                                            CGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGACATACGTACG               >  SRR4302537.3047550/1‑94 (MQ=60)
                                                                             GGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGG              >  SRR4302537.1792987/1‑94 (MQ=60)
                                                                              GCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGC             >  SRR4302537.220151/1‑94 (MQ=60)
                                                                                TGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGT           >  SRR4302537.4190323/1‑94 (MQ=60)
                                                                                    GAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACG       <  SRR4302537.128768/94‑1 (MQ=60)
                                                                                     AGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGT      <  SRR4302537.5966948/94‑1 (MQ=60)
                                                                                      GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG     <  SRR4302537.4305855/94‑1 (MQ=60)
                                                                                      GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG     <  SRR4302537.1523477/94‑1 (MQ=60)
                                                                                      GTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTG     <  SRR4302537.2070025/94‑1 (MQ=60)
                                                                                        CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   >  SRR4302537.2929099/1‑94 (MQ=60)
                                                                                        CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   <  SRR4302537.1781478/94‑1 (MQ=60)
                                                                                        CGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG   <  SRR4302537.5959400/94‑1 (MQ=60)
                                                                                        CGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAACCCATCACCGGACGCTGTTTCATCAGGGCAGCCATACGTACGGCGTTACGTGCG   <  SRR4302537.4082765/94‑1 (MQ=60)
                                                                                         GGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  <  SRR4302537.2326380/94‑1 (MQ=60)
                                                                                           |                                                                                           
CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGT  >  NC_000913/3078154‑3078336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑