Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,205,532	T→C	L174P (CTG→CCG)	ttdB →	L‑tartrate dehydratase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,205,532	0	T	C	99.2%	804.9 / ‑5.3	234	L174P (CTG→CCG)	ttdB	L‑tartrate dehydratase subunit B
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (119/113); minor base A (1/1); total (120/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 241 total aligned reads displayed.

CAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT  >  NC_000913/3205442‑3205621

cAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGa                                                                                         >  2:3797061/1‑93 (MQ=255)

 agagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGAt                                                                                        <  2:218946/93‑1 (MQ=255)

  gagCTCGGAGTGCCGGAGTCACTGTGCGTCTGCCGGGTCAACGAGTCCGCCCCGCTGATTGTCTCTATTGATCCCCACGGCAACAACCCGATa                                                                                       <  2:2637754/93‑1 (MQ=255)

  gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATa                                                                                       >  1:4407762/1‑93 (MQ=255)

   agCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAg                                                                                      <  2:5477648/93‑1 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     >  2:2533719/1‑93 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     >  2:4484692/1‑93 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     <  1:4816858/93‑1 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     >  2:6097319/1‑93 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     <  2:3554360/93‑1 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     <  1:2287602/93‑1 (MQ=255)

    gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGc                                                                                     <  2:268756/93‑1 (MQ=255)

     cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGcc                                                                                    >  1:3932711/1‑93 (MQ=255)

      tCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCg                                                                                   <  2:777838/93‑1 (MQ=255)

       cGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGa                                                                                  >  2:4496343/1‑93 (MQ=255)

         gAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGaaa                                                                                <  2:2292056/93‑1 (MQ=255)

         gAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGaaa                                                                                >  2:4675189/1‑93 (MQ=255)

          aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGaaaa                                                                               <  2:2370887/93‑1 (MQ=255)

             gCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaa                                                                            >  1:5724218/1‑93 (MQ=255)

             gCCGGACTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaa                                                                            <  1:1718679/93‑1 (MQ=255)

              ccGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaaa                                                                           >  2:4135969/1‑93 (MQ=255)

                  aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGc                                                                       <  2:1320022/93‑1 (MQ=255)

                  aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGc                                                                       <  1:4637184/93‑1 (MQ=255)

                   gTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATCCCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCt                                                                      <  1:1965804/93‑1 (MQ=255)

                    tCACTGTGGGCATGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTg                                                                     <  1:5164865/93‑1 (MQ=255)

                     cACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGt                                                                    <  2:3605782/93‑1 (MQ=255)

                      aCTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGtt                                                                   <  1:377574/93‑1 (MQ=255)

                      aCTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTAATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGtt                                                                   >  1:5529939/1‑93 (MQ=255)

                       cTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTc                                                                  <  1:2124676/93‑1 (MQ=255)

                        tgtgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCg                                                                 <  1:3540985/93‑1 (MQ=255)

                         gtgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGc                                                                >  1:4006088/1‑93 (MQ=255)

                             gTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                            >  1:1399777/1‑93 (MQ=255)

                             gTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                            <  1:3152345/93‑1 (MQ=255)

                             gTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                            >  1:1370096/1‑93 (MQ=255)

                             gTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                            <  1:2645906/93‑1 (MQ=255)

                                tGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                         <  1:5253036/93‑1 (MQ=255)

                                     ggTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGa                                                    >  2:5022528/1‑93 (MQ=255)

                                      gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGAt                                                   >  2:5752907/1‑93 (MQ=255)

                                      gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGAt                                                   >  1:1666736/1‑93 (MQ=255)

                                        cAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATcc                                                 <  1:1110491/93‑1 (MQ=255)

                                         aaaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATccc                                                >  1:3188697/1‑93 (MQ=255)

                                         aaaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATccc                                                >  2:2741955/1‑93 (MQ=255)

                                          aaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                               >  2:3322402/1‑93 (MQ=255)

                                           agagTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCAt                                              <  2:1977206/93‑1 (MQ=255)

                                            gagTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATc                                             >  1:4967475/1‑93 (MQ=255)

                                             agTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCg                                            >  1:1004413/1‑93 (MQ=255)

                                               ttCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACACCCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTg                                          <  2:611585/93‑1 (MQ=255)

                                               ttCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTg                                          >  2:4609340/1‑93 (MQ=255)

                                                 cGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGa                                        <  2:683871/93‑1 (MQ=255)

                                                  ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                       <  1:5390920/93‑1 (MQ=255)

                                                  ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                       <  1:4217607/93‑1 (MQ=255)

                                                  ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                       >  1:5673030/1‑93 (MQ=255)

                                                   gCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                                      >  1:5239580/1‑93 (MQ=255)

                                                    cccGCTGATTGTCTCTATTGATACCCACGGCAACACCCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAaga                                     <  1:698607/93‑1 (MQ=255)

                                                    cccGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAaga                                     <  1:2900258/93‑1 (MQ=255)

                                                     ccGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAagag                                    <  1:4475869/93‑1 (MQ=255)

                                                      cGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAgaga                                   <  1:2489938/93‑1 (MQ=255)

                                                      cGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAgaga                                   >  2:1627319/1‑93 (MQ=255)

                                                      cGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAgaga                                   >  1:1343948/1‑93 (MQ=255)

                                                      cGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCACATCGCGGAAgaga                                   >  2:3010449/1‑93 (MQ=255)

                                                       gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                  >  1:6181508/1‑93 (MQ=255)

                                                       gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                  <  2:3640875/93‑1 (MQ=255)

                                                       gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                  >  2:3688015/1‑93 (MQ=255)

                                                       gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                  >  1:1461087/1‑93 (MQ=255)

                                                        cTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATc                                 <  2:1867865/93‑1 (MQ=255)

                                                         tGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                <  1:1537694/93‑1 (MQ=255)

                                                          gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTg                               <  2:907254/93‑1 (MQ=255)

                                                          gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTg                               >  1:5900691/1‑93 (MQ=255)

                                                           aTTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                              >  1:5130691/1‑93 (MQ=255)

                                                           aTTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                              <  1:5325110/93‑1 (MQ=255)

                                                            ttGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                             <  2:2951264/93‑1 (MQ=255)

                                                            ttGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                             >  1:4882973/1‑93 (MQ=255)

                                                            ttGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                             <  1:5953982/93‑1 (MQ=255)

                                                              gTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATGTGCGAg                           >  1:1496842/1‑93 (MQ=255)

                                                               tctcTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                          >  1:4765568/1‑93 (MQ=255)

                                                                 tctATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                        <  2:1540106/93‑1 (MQ=255)

                                                                 tctATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                        >  1:80041/1‑93 (MQ=255)

                                                                 tctATTGATACCAACGGCAACAACCCGATAGCCGAAAGCAAAAAGCTGTTCGCCGCACGCCCCGATCCCATCGTGGAAGAGATCTGCGAGCAc                        >  2:6360684/1‑93 (MQ=255)

                                                                  ctATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACg                       <  2:4881388/93‑1 (MQ=255)

                                                                   tATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGt                      >  1:4844827/1‑93 (MQ=255)

                                                                   tATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGt                      >  2:1075389/1‑93 (MQ=255)

                                                                     ttGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTcc                    <  1:2797570/93‑1 (MQ=255)

                                                                     ttGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTcc                    >  2:3893554/1‑93 (MQ=255)

                                                                     ttGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTcc                    >  2:4847906/1‑93 (MQ=255)

                                                                      tGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCa                   >  2:4405793/1‑93 (MQ=255)

                                                                         tACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                <  2:3150692/93‑1 (MQ=255)

                                                                          aCCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTAc               <  1:4366116/93‑1 (MQ=255)

                                                                           cccACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACa              >  2:5387409/1‑93 (MQ=255)

                                                                           cccACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACa              <  1:3041651/93‑1 (MQ=255)

                                                                            ccACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAt             >  1:3584718/1‑93 (MQ=255)

                                                                              aCGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa           >  2:4156763/1‑93 (MQ=255)

                                                                                ggCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa         <  1:3647282/93‑1 (MQ=255)

                                                                                cgCAACAACCCGATAGCCGAAAACACAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa         <  1:6247136/92‑1 (MQ=255)

                                                                                 gCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAt        >  1:3429536/1‑93 (MQ=255)

                                                                                  caacaaCCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa       <  1:909782/93‑1 (MQ=255)

                                                                                   aacaacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATaa      >  1:1837235/1‑93 (MQ=255)

                                                                                    acaacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAc     <  1:2024891/93‑1 (MQ=255)

                                                                                     caacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAcc    <  2:1780290/93‑1 (MQ=255)

                                                                                      aacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAccc   <  1:3358622/93‑1 (MQ=255)

                                                                                       acCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCt  >  2:3471839/1‑93 (MQ=255)

CAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT  >  NC_000913/3205442‑3205621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCG  >  NC_000913/3205439‑3205625

GGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCC                                                                                               <  SRR4302537.6311968/94‑1 (MQ=60)

GGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCC                                                                                               <  SRR4302537.2866800/94‑1 (MQ=60)

 GACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCG                                                                                              <  SRR4302537.5233336/94‑1 (MQ=60)

 GACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCG                                                                                              <  SRR4302537.2553399/94‑1 (MQ=60)

   CAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGAT                                                                                            <  SRR4302537.1986954/94‑1 (MQ=60)

    AGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATA                                                                                           >  SRR4302537.4438183/1‑94 (MQ=60)

      AGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGC                                                                                         >  SRR4302537.4092533/1‑94 (MQ=60)

      AGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGC                                                                                         >  SRR4302537.2717898/1‑94 (MQ=60)

       GCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCC                                                                                        >  SRR4302537.3960192/1‑94 (MQ=60)

       GCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCC                                                                                        <  SRR4302537.2303737/94‑1 (MQ=60)

       GCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCC                                                                                        <  SRR4302537.4850118/94‑1 (MQ=60)

        CTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCAGATAGCCG                                                                                       <  SRR4302537.3705203/94‑1 (MQ=60)

          CGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAA                                                                                     <  SRR4302537.4376406/94‑1 (MQ=60)

          CGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAA                                                                                     >  SRR4302537.2192284/1‑94 (MQ=60)

          CGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAA                                                                                     >  SRR4302537.5276150/1‑94 (MQ=60)

           GGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAA                                                                                    >  SRR4302537.781475/1‑94 (MQ=60)

            GAATGCCGGAGTCACTGTGGGTCCGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAA                                                                                   >  SRR4302537.543150/1‑94 (MQ=60)

               TGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAA                                                                                >  SRR4302537.466593/1‑94 (MQ=60)

               TGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAA                                                                                >  SRR4302537.5763401/1‑94 (MQ=60)

                GCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAA                                                                               <  SRR4302537.4971105/94‑1 (MQ=60)

                GCCGGACTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAA                                                                               <  SRR4302537.1730692/94‑1 (MQ=60)

                 CCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAA                                                                              <  SRR4302537.4890228/94‑1 (MQ=60)

                  CGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAA                                                                             >  SRR4302537.3714741/1‑94 (MQ=60)

                  CGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAA                                                                             <  SRR4302537.2526201/94‑1 (MQ=60)

                  CGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAA                                                                             >  SRR4302537.480696/1‑94 (MQ=60)

                  CGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGCTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAA                                                                             >  SRR4302537.3108960/1‑94 (MQ=60)

                     AGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCT                                                                          <  SRR4302537.4669257/94‑1 (MQ=60)

                      GTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATCCCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTG                                                                         <  SRR4302537.1979472/94‑1 (MQ=60)

                      GTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTG                                                                         >  SRR4302537.1215024/1‑94 (MQ=60)

                       TCACTGTGGGCATGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGT                                                                        <  SRR4302537.5200454/94‑1 (MQ=60)

                        CACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTT                                                                       <  SRR4302537.3662652/94‑1 (MQ=60)

                        CACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTAATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTT                                                                       >  SRR4302537.5567688/1‑94 (MQ=60)

                         ACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTC                                                                      >  SRR4302537.3251525/1‑94 (MQ=60)

                         ACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTC                                                                      <  SRR4302537.380192/94‑1 (MQ=60)

                          CTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCG                                                                     <  SRR4302537.2139621/94‑1 (MQ=60)

                          CTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCG                                                                     <  SRR4302537.3164384/94‑1 (MQ=60)

                           TGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGC                                                                    >  SRR4302537.4034125/1‑94 (MQ=60)

                           TGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGC                                                                    <  SRR4302537.3565996/94‑1 (MQ=60)

                            GTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCC                                                                   >  SRR4302537.2459255/1‑94 (MQ=60)

                            GTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCC                                                                   <  SRR4302537.3568772/94‑1 (MQ=60)

                             TGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCG                                                                  <  SRR4302537.294069/94‑1 (MQ=60)

                              GGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGCTAGCCGAAAACAAAAAGCTGTTCGCCGA                                                                 >  SRR4302537.6262623/1‑94 (MQ=60)

                               GGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAA                                                                >  SRR4302537.1409536/1‑94 (MQ=60)

                               GGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAA                                                                >  SRR4302537.1460725/1‑94 (MQ=60)

                               GGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAA                                                                >  SRR4302537.1379641/1‑94 (MQ=60)

                                GTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                               <  SRR4302537.2664410/94‑1 (MQ=60)

                                GTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                               <  SRR4302537.3174479/94‑1 (MQ=60)

                                GTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                               <  SRR4302537.4726998/94‑1 (MQ=60)

                                GTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                               <  SRR4302537.5204553/94‑1 (MQ=60)

                                 TCTGCGGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACG                                                              <  SRR4302537.6075445/94‑1 (MQ=60)

                                 TCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACG                                                              <  SRR4302537.3506948/94‑1 (MQ=60)

                                  CTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGC                                                             >  SRR4302537.2837413/1‑94 (MQ=60)

                                   TGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCC                                                            <  SRR4302537.5289183/94‑1 (MQ=60)

                                    GCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCG                                                           >  SRR4302537.4728237/1‑94 (MQ=60)

                                     CCGGGACAAAGGGTTCGGCCCGCTGATTTTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGC                                                          <  SRR4302537.1747924/94‑1 (MQ=60)

                                        GGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGAT                                                       >  SRR4302537.1678411/1‑94 (MQ=60)

                                         GTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATC                                                      >  SRR4302537.6460761/1‑94 (MQ=60)

                                           CAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCC                                                    <  SRR4302537.1118162/94‑1 (MQ=60)

                                           CAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCC                                                    >  SRR4302537.1790683/1‑94 (MQ=60)

                                           CAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCC                                                    >  SRR4302537.4404327/1‑94 (MQ=60)

                                           CAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCC                                                    >  SRR4302537.4404328/1‑94 (MQ=60)

                                           CAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCC                                                    >  SRR4302537.3211125/1‑94 (MQ=60)

                                            AAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCA                                                   >  SRR4302537.5358118/1‑94 (MQ=60)

                                              AGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATC                                                 >  SRR4302537.5001712/1‑94 (MQ=60)

                                              AGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATC                                                 >  SRR4302537.1443331/1‑94 (MQ=60)

                                               GAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCG                                                >  SRR4302537.1011346/1‑94 (MQ=60)

                                                AGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGT                                               >  SRR4302537.519804/1‑94 (MQ=60)

                                                AGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGT                                               >  SRR4302537.5708231/1‑94 (MQ=60)

                                                   TCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGA                                            <  SRR4302537.5619463/94‑1 (MQ=60)

                                                    CGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAA                                           >  SRR4302537.5711847/1‑94 (MQ=60)

                                                    CGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAA                                           <  SRR4302537.5774660/94‑1 (MQ=60)

                                                     GGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAG                                          >  SRR4302537.5275635/1‑94 (MQ=60)

                                                     GGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAG                                          <  SRR4302537.5427747/94‑1 (MQ=60)

                                                     GGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAG                                          <  SRR4302537.871464/94‑1 (MQ=60)

                                                     GGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAG                                          <  SRR4302537.4247006/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACACCCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        <  SRR4302537.703391/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        <  SRR4302537.79591/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        <  SRR4302537.1059195/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        >  SRR4302537.2074291/1‑94 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        <  SRR4302537.2920635/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        >  SRR4302537.1374240/1‑94 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        <  SRR4302537.1673521/94‑1 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        >  SRR4302537.3210486/1‑94 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAG                                        >  SRR4302537.148724/1‑94 (MQ=60)

                                                       CCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCACATCGTGGAAGAG                                        <  SRR4302537.4229403/94‑1 (MQ=60)

                                                        CCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGA                                       >  SRR4302537.5831573/1‑94 (MQ=60)

                                                        CCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGA                                       >  SRR4302537.1554708/1‑94 (MQ=60)

                                                        CCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGA                                       >  SRR4302537.1353310/1‑94 (MQ=60)

                                                        CCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGA                                       <  SRR4302537.3951371/94‑1 (MQ=60)

                                                        CCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGA                                       <  SRR4302537.4506833/94‑1 (MQ=60)

                                                         CGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAT                                      <  SRR4302537.2507472/94‑1 (MQ=60)

                                                         CGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAT                                      >  SRR4302537.1471254/1‑94 (MQ=60)

                                                         CGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAT                                      >  SRR4302537.6224086/1‑94 (MQ=60)

                                                          GCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATC                                     >  SRR4302537.485198/1‑94 (MQ=60)

                                                          GCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATC                                     >  SRR4302537.5425002/1‑94 (MQ=60)

                                                           CTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCT                                    >  SRR4302537.5748315/1‑94 (MQ=60)

                                                           CTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCT                                    >  SRR4302537.1375941/1‑94 (MQ=60)

                                                           CTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTACGCCGAACGCCGCGATCCCATCGTGGAAGAGATCT                                    <  SRR4302537.4469887/94‑1 (MQ=60)

                                                            TGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTG                                   <  SRR4302537.1548431/94‑1 (MQ=60)

                                                            TGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTG                                   >  SRR4302537.5941183/1‑94 (MQ=60)

                                                             GATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGC                                  >  SRR4302537.5166049/1‑94 (MQ=60)

                                                              ATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                 >  SRR4302537.3981516/1‑94 (MQ=60)

                                                              ATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                 <  SRR4302537.5361651/94‑1 (MQ=60)

                                                              ATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                 >  SRR4302537.1402395/1‑94 (MQ=60)

                                                              ATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                 >  SRR4302537.4916700/1‑94 (MQ=60)

                                                              ATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                 >  SRR4302537.502216/1‑94 (MQ=60)

                                                               TTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGA                                <  SRR4302537.5994855/94‑1 (MQ=60)

                                                                TGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATGTGCGAG                               >  SRR4302537.1507284/1‑94 (MQ=60)

                                                                TGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAG                               >  SRR4302537.5314724/1‑94 (MQ=60)

                                                                 GTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGC                              <  SRR4302537.2849133/94‑1 (MQ=60)

                                                                 GTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGC                              >  SRR4302537.4798477/1‑94 (MQ=60)

                                                                 GTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGC                              >  SRR4302537.4873752/1‑94 (MQ=60)

                                                                   CTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAC                            >  SRR4302537.80627/1‑94 (MQ=60)

                                                                   CTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAC                            >  SRR4302537.6263114/1‑94 (MQ=60)

                                                                    TCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACG                           <  SRR4302537.2079864/94‑1 (MQ=60)

                                                                     CTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGT                          <  SRR4302537.5253892/94‑1 (MQ=60)

                                                                     CTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGT                          >  SRR4302537.4878260/1‑94 (MQ=60)

                                                                      TATTGATACCCACGGCAACACCCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTC                         <  SRR4302537.248102/94‑1 (MQ=60)

                                                                        TTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCA                       <  SRR4302537.2817132/94‑1 (MQ=60)

                                                                           ATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTA                    >  SRR4302537.1903584/1‑94 (MQ=60)

                                                                             ACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACA                  <  SRR4302537.4396301/94‑1 (MQ=60)

                                                                              CCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAT                 <  SRR4302537.5104289/94‑1 (MQ=60)

                                                                              CCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAT                 >  SRR4302537.3609988/1‑94 (MQ=60)

                                                                              CCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAT                 <  SRR4302537.3062845/94‑1 (MQ=60)

                                                                                   GGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAT            >  SRR4302537.3453808/1‑94 (MQ=60)

                                                                                   GGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAT            <  SRR4302537.3672956/94‑1 (MQ=60)

                                                                                   GGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAT            >  SRR4302537.5942603/1‑94 (MQ=60)

                                                                                   CGCAACAACCCGATAGCCGAAAACACAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAT            <  SRR4302537.6290062/94‑1 (MQ=60)

                                                                                     CAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAA          <  SRR4302537.916062/94‑1 (MQ=60)

                                                                                     CAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAA          >  SRR4302537.1850025/1‑94 (MQ=60)

                                                                                      AACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGACCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAC         <  SRR4302537.1693306/94‑1 (MQ=60)

                                                                                       ACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACC        >  SRR4302537.336502/1‑94 (MQ=60)

                                                                                       ACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACC        <  SRR4302537.2039014/94‑1 (MQ=60)

                                                                                        CAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCC       >  SRR4302537.1512959/1‑94 (MQ=60)

                                                                                        CAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCC       <  SRR4302537.3591000/94‑1 (MQ=60)

                                                                                         AACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT      <  SRR4302537.3382412/94‑1 (MQ=60)

                                                                                         AACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT      >  SRR4302537.3396247/1‑94 (MQ=60)

                                                                                          ACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTC     >  SRR4302537.2002792/1‑94 (MQ=60)

                                                                                          ACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTC     >  SRR4302537.2453986/1‑94 (MQ=60)

                                                                                            CCGATAGCCGAAAACAAAAATCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCC   <  SRR4302537.3528746/94‑1 (MQ=60)

                                                                                            CCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCC   >  SRR4302537.1425101/1‑94 (MQ=60)

                                                                                             CGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCATGTCCATTACATCAAATAACCCTCCCG  >  SRR4302537.702207/1‑94 (MQ=60)

                                                                                             CGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCG  <  SRR4302537.5642243/94‑1 (MQ=60)

GGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCG  >  NC_000913/3205439‑3205625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`