Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,212,115	T→C	R476R (CGT→CGC)	rpoD →	RNA polymerase sigma(70)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,212,115	0	T	C	100.0%	863.7 / NA	248	R476R (CGT→CGC)	rpoD	RNA polymerase sigma(70)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (138/110); total (138/110)

Only 100 of 257 total aligned reads displayed.

GGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCT  >  NC_000913/3212025‑3212203

ggCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGa                                                                                        >  2:1573861/1‑93 (MQ=255)

  cgcgcACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAAc                                                                                      <  2:356162/93‑1 (MQ=255)

   gcgcACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAAcc                                                                                     >  2:5788956/1‑93 (MQ=255)

     gccccATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGa                                                                                   <  2:272803/90‑1 (MQ=255)

     gcACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGa                                                                                   >  2:4946164/1‑93 (MQ=255)

       aCCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACg                                                                                 >  1:1101133/1‑93 (MQ=255)

       aCCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACg                                                                                 <  1:4188924/93‑1 (MQ=255)

       aCCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACg                                                                                 >  2:1513219/1‑93 (MQ=255)

        ccATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGc                                                                                <  1:4750074/93‑1 (MQ=255)

         cATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGcc                                                                               >  2:5403160/1‑93 (MQ=255)

         cATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGcc                                                                               <  2:934367/93‑1 (MQ=255)

          aTCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCg                                                                              >  2:3348392/1‑93 (MQ=255)

          aTCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCg                                                                              <  1:1315425/93‑1 (MQ=255)

           tCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCgg                                                                             >  1:1486236/1‑93 (MQ=255)

           tCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCgg                                                                             >  2:3148433/1‑93 (MQ=255)

           tCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCgg                                                                             >  2:1355094/1‑93 (MQ=255)

           tCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCgg                                                                             >  1:5469781/1‑93 (MQ=255)

            ccGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGga                                                                            >  1:5888886/1‑93 (MQ=255)

             cGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGgaa                                                                           <  2:3270741/93‑1 (MQ=255)

               tATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGgaaga                                                                         >  1:6339226/1‑93 (MQ=255)

               tATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGgaaga                                                                         <  1:1135389/93‑1 (MQ=255)

                aTTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGgaagaa                                                                        >  1:558607/1‑93 (MQ=255)

                   ccGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTg                                                                     <  2:6038472/93‑1 (MQ=255)

                   ccGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTg                                                                     >  2:5549994/1‑93 (MQ=255)

                      gTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCt                                                                  >  1:2200010/1‑93 (MQ=255)

                      gTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCt                                                                  >  1:4157397/1‑93 (MQ=255)

                        gCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGa                                                                >  1:4652038/1‑93 (MQ=255)

                           tatGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACg                                                             >  1:1202949/1‑93 (MQ=255)

                           taagATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACg                                                             <  2:2954333/90‑1 (MQ=255)

                            atGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGt                                                            <  1:4079882/93‑1 (MQ=255)

                             tGATTGAGACCATCAACAAGCTCACCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTa                                                           <  1:3337285/93‑1 (MQ=255)

                             tGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTa                                                           <  1:211621/93‑1 (MQ=255)

                              gATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAt                                                          <  2:1738883/93‑1 (MQ=255)

                                ttGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGc                                                        <  2:4502954/93‑1 (MQ=255)

                                 tGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCt                                                       >  2:4608584/1‑93 (MQ=255)

                                 tGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCt                                                       >  1:627646/1‑93 (MQ=255)

                                   agaCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGa                                                     >  2:4129013/1‑93 (MQ=255)

                                    gaCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAt                                                    >  1:2679592/1‑93 (MQ=255)

                                    gaCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAt                                                    >  2:5064872/1‑93 (MQ=255)

                                    gaCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAt                                                    >  1:2977504/1‑93 (MQ=255)

                                     aCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATg                                                   >  2:3763975/1‑93 (MQ=255)

                                      ccATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGc                                                  <  1:5074955/93‑1 (MQ=255)

                                      ccAACAACGAGCTCATACGTATTGCACGCCAGATGCTGCAATAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGc                                                  <  2:4540214/93‑1 (MQ=255)

                                        aTCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCg                                                <  1:1960849/93‑1 (MQ=255)

                                        aTCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCg                                                >  2:2270139/1‑93 (MQ=255)

                                         tCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCgg                                               >  2:5627698/1‑93 (MQ=255)

                                           aacaaGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGaa                                             <  1:2651927/93‑1 (MQ=255)

                                              aaGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGAc                                          >  2:496771/1‑93 (MQ=255)

                                               aGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACa                                         <  2:2106036/93‑1 (MQ=255)

                                                 cTCAGCCGTATTTCTCGCCAGATGCGGCAAGAGATGGCCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAg                                       <  2:1597359/93‑1 (MQ=255)

                                                 cTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAg                                       <  1:795024/93‑1 (MQ=255)

                                                     aCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATcc                                   <  2:4073191/93‑1 (MQ=255)

                                                     aCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATcc                                   >  2:2159471/1‑93 (MQ=255)

                                                     aCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATcc                                   <  1:730919/93‑1 (MQ=255)

                                                     aCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATcc                                   >  1:4380635/1‑93 (MQ=255)

                                                     aCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATcc                                   <  1:257274/93‑1 (MQ=255)

                                                      ccGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCg                                  >  1:3800837/1‑93 (MQ=255)

                                                      ccGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCg                                  >  2:5136041/1‑93 (MQ=255)

                                                        gTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCa                                >  1:3516141/1‑93 (MQ=255)

                                                        gTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCa                                >  1:139193/1‑93 (MQ=255)

                                                        gTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCa                                >  1:1509433/1‑93 (MQ=255)

                                                         tATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCaa                               >  1:1176181/1‑93 (MQ=255)

                                                         tATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCaa                               >  2:2034005/1‑93 (MQ=255)

                                                          aTTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCaaa                              <  1:5030767/93‑1 (MQ=255)

                                                           tttCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAg                             >  2:5998857/1‑93 (MQ=255)

                                                           tttCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAg                             <  2:5424225/93‑1 (MQ=255)

                                                            ttCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGt                            <  1:920285/93‑1 (MQ=255)

                                                             tctcGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTg                           >  2:4456121/1‑93 (MQ=255)

                                                              ctcGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCAGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGc                          <  1:2876774/93‑1 (MQ=255)

                                                               tcGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCt                         >  2:853258/1‑93 (MQ=255)

                                                               tcGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCt                         <  2:2541012/93‑1 (MQ=255)

                                                               tcGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCt                         <  2:4821624/93‑1 (MQ=255)

                                                                  ccAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGaa                      <  1:2049527/93‑1 (MQ=255)

                                                                   cAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAg                     >  1:6413515/1‑93 (MQ=255)

                                                                    aGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGa                    >  1:2362277/1‑93 (MQ=255)

                                                                     gATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAt                   <  2:1866239/93‑1 (MQ=255)

                                                                     gATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAt                   >  1:4024012/1‑93 (MQ=255)

                                                                     gATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAt                   >  1:5428630/1‑93 (MQ=255)

                                                                     gATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAt                   <  1:4920756/93‑1 (MQ=255)

                                                                      aTGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAtg                  >  1:6208669/1‑92 (MQ=255)

                                                                      aTGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATc                  <  1:5544232/93‑1 (MQ=255)

                                                                        gctgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGc                <  1:4474790/93‑1 (MQ=255)

                                                                         ctgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGca               >  1:5171723/1‑92 (MQ=255)

                                                                         ctgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCAcc               >  1:5704669/1‑93 (MQ=255)

                                                                          tgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCa              >  2:2509951/1‑93 (MQ=255)

                                                                          tgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCa              <  1:1769854/93‑1 (MQ=255)

                                                                          tgcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCa              <  2:1727907/93‑1 (MQ=255)

                                                                           gcAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCaa             <  2:6207913/93‑1 (MQ=255)

                                                                             aaGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAag           <  2:6393154/93‑1 (MQ=255)

                                                                              agagATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAaga          <  2:3054662/93‑1 (MQ=255)

                                                                              agagATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAaga          >  2:2626982/1‑93 (MQ=255)

                                                                              agagATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAaga          >  2:2360021/1‑93 (MQ=255)

                                                                              agagATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAaga          >  1:6073566/1‑93 (MQ=255)

                                                                              agagATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCAAAaga          >  2:5923347/1‑93 (MQ=255)

                                                                               gagaTGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAagag         <  1:2493990/93‑1 (MQ=255)

                                                                               gagaTGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAagag         >  1:1327090/1‑93 (MQ=255)

                                                                                   tGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCaa     >  2:4266156/1‑93 (MQ=255)

                                                                                   tGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCaa     >  1:1685333/1‑93 (MQ=255)

                                                                                    gggCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAAt    <  2:536829/93‑1 (MQ=255)

                                                                                      gCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAAtct  <  2:52314/93‑1 (MQ=255)

GGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCT  >  NC_000913/3212025‑3212203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATG  >  NC_000913/3212026‑3212208

GCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAAC                                                                                           <  SRR4302537.2540573/94‑1 (MQ=60)

  GCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCG                                                                                         >  SRR4302537.2499569/1‑94 (MQ=60)

    GCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGAC                                                                                       <  SRR4302537.1510348/94‑1 (MQ=60)

     CACCATCCGTATTCCGGTGCATATTATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACG                                                                                      >  SRR4302537.4355793/1‑94 (MQ=60)

     CACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACG                                                                                      >  SRR4302537.1108744/1‑94 (MQ=60)

      ACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGC                                                                                     <  SRR4302537.4218178/94‑1 (MQ=60)

       CCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCC                                                                                    <  SRR4302537.4782886/94‑1 (MQ=60)

       CCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCC                                                                                    >  SRR4302537.5454999/1‑94 (MQ=60)

        CATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCG                                                                                   <  SRR4302537.4710394/94‑1 (MQ=60)

        CATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCG                                                                                   <  SRR4302537.285399/94‑1 (MQ=60)

         ATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGG                                                                                  >  SRR4302537.1496579/1‑94 (MQ=60)

         ATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGG                                                                                  >  SRR4302537.5507091/1‑94 (MQ=60)

         ATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGG                                                                                  <  SRR4302537.1324587/94‑1 (MQ=60)

          TCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGA                                                                                 >  SRR4302537.5929292/1‑94 (MQ=60)

           CCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAA                                                                                <  SRR4302537.5537913/94‑1 (MQ=60)

           CCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAA                                                                                >  SRR4302537.5910491/1‑94 (MQ=60)

            CGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAG                                                                               >  SRR4302537.4799299/1‑94 (MQ=60)

             GTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGA                                                                              >  SRR4302537.6382545/1‑94 (MQ=60)

              TATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAA                                                                             >  SRR4302537.562452/1‑94 (MQ=60)

              TATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAA                                                                             <  SRR4302537.1143277/94‑1 (MQ=60)

                TTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACT                                                                           <  SRR4302537.2132669/94‑1 (MQ=60)

                TTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACT                                                                           <  SRR4302537.1133454/94‑1 (MQ=60)

                TTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACT                                                                           >  SRR4302537.1986884/1‑94 (MQ=60)

                  CCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGG                                                                         <  SRR4302537.2988125/94‑1 (MQ=60)

                   CGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGC                                                                        >  SRR4302537.5995169/1‑94 (MQ=60)

                   CGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGC                                                                        >  SRR4302537.5586733/1‑94 (MQ=60)

                    GGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCT                                                                       >  SRR4302537.2215517/1‑94 (MQ=60)

                    GGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCT                                                                       >  SRR4302537.4186430/1‑94 (MQ=60)

                    GGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCAGGAAGAACTGGCT                                                                       <  SRR4302537.4428101/94‑1 (MQ=60)

                     GTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTG                                                                      >  SRR4302537.3552375/1‑94 (MQ=60)

                      TGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGA                                                                     >  SRR4302537.4684203/1‑94 (MQ=60)

                        CATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAAC                                                                   >  SRR4302537.6147074/1‑94 (MQ=60)

                        CATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAAC                                                                   <  SRR4302537.5487344/94‑1 (MQ=60)

                         ATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACG                                                                  >  SRR4302537.1211314/1‑94 (MQ=60)

                           ATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTA                                                                <  SRR4302537.4108365/94‑1 (MQ=60)

                            TGATTGAGACCATCAACAAGCTCACCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAT                                                               <  SRR4302537.3360907/94‑1 (MQ=60)

                            TGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAT                                                               <  SRR4302537.1723979/94‑1 (MQ=60)

                            TGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAT                                                               <  SRR4302537.213133/94‑1 (MQ=60)

                            TGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAT                                                               <  SRR4302537.5344528/94‑1 (MQ=60)

                            TGATAGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTAT                                                               <  SRR4302537.4363790/94‑1 (MQ=60)

                             GATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATG                                                              <  SRR4302537.1312442/94‑1 (MQ=60)

                               TTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCT                                                            >  SRR4302537.631921/1‑94 (MQ=60)

                                  AGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAT                                                         >  SRR4302537.2698303/1‑94 (MQ=60)

                                  AGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAT                                                         <  SRR4302537.5676204/94‑1 (MQ=60)

                                  AGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAT                                                         >  SRR4302537.2998337/1‑94 (MQ=60)

                                   GACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATG                                                        >  SRR4302537.704345/1‑94 (MQ=60)

                                   GACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATG                                                        >  SRR4302537.1194918/1‑94 (MQ=60)

                                   AACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATG                                                        <  SRR4302537.1487653/94‑1 (MQ=60)

                                    ACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGC                                                       >  SRR4302537.1231001/1‑94 (MQ=60)

                                     CCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCC                                                      >  SRR4302537.5136213/1‑94 (MQ=60)

                                     CCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCC                                                      >  SRR4302537.1168040/1‑94 (MQ=60)

                                     CCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCC                                                      <  SRR4302537.5109894/94‑1 (MQ=60)

                                       ATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGG                                                    <  SRR4302537.1974486/94‑1 (MQ=60)

                                       ATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGG                                                    >  SRR4302537.4195106/1‑94 (MQ=60)

                                       ATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGG                                                    <  SRR4302537.5000348/94‑1 (MQ=60)

                                       ATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGG                                                    >  SRR4302537.2851630/1‑94 (MQ=60)

                                       ATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGG                                                    <  SRR4302537.1836937/94‑1 (MQ=60)

                                         CAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAA                                                  >  SRR4302537.6443606/1‑94 (MQ=60)

                                         CAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAA                                                  >  SRR4302537.2072173/1‑94 (MQ=60)

                                         CAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAA                                                  >  SRR4302537.2123604/1‑94 (MQ=60)

                                          AACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAG                                                 <  SRR4302537.2670473/94‑1 (MQ=60)

                                            CAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGAC                                               >  SRR4302537.4059518/1‑94 (MQ=60)

                                             AAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACA                                              >  SRR4302537.1585579/1‑94 (MQ=60)

                                             AAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACA                                              <  SRR4302537.3984663/94‑1 (MQ=60)

                                              AGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAA                                             >  SRR4302537.774285/1‑94 (MQ=60)

                                               GCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAG                                            >  SRR4302537.1588310/1‑94 (MQ=60)

                                                CTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGA                                           <  SRR4302537.800479/94‑1 (MQ=60)

                                                   AACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCC                                        >  SRR4302537.2417/1‑94 (MQ=60)

                                                   AACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCC                                        >  SRR4302537.4410870/1‑94 (MQ=60)

                                                    ACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCG                                       <  SRR4302537.735909/94‑1 (MQ=60)

                                                    ACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCG                                       >  SRR4302537.3827504/1‑94 (MQ=60)

                                                    ACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCG                                       <  SRR4302537.259096/94‑1 (MQ=60)

                                                     CCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGC                                      <  SRR4302537.3907635/94‑1 (MQ=60)

                                                      CGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCA                                     >  SRR4302537.140186/1‑94 (MQ=60)

                                                      CGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCA                                     >  SRR4302537.1519989/1‑94 (MQ=60)

                                                      CGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCA                                     >  SRR4302537.3541017/1‑94 (MQ=60)

                                                      CGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCA                                     >  SRR4302537.3237829/1‑94 (MQ=60)

                                                      CGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCA                                     >  SRR4302537.3247886/1‑94 (MQ=60)

                                                       GTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAA                                    >  SRR4302537.4147367/1‑94 (MQ=60)

                                                       GTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAA                                    >  SRR4302537.1184361/1‑94 (MQ=60)

                                                       GTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAA                                    >  SRR4302537.2694104/1‑94 (MQ=60)

                                                         ATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAG                                  >  SRR4302537.2668337/1‑94 (MQ=60)

                                                         ATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAG                                  <  SRR4302537.5089702/94‑1 (MQ=60)

                                                         ATTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAG                                  <  SRR4302537.5065403/94‑1 (MQ=60)

                                                          TTTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGT                                 <  SRR4302537.4515397/94‑1 (MQ=60)

                                                           TTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACTGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTG                                <  SRR4302537.864195/94‑1 (MQ=60)

                                                           TTCTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTG                                <  SRR4302537.926637/94‑1 (MQ=60)

                                                             CTCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCAGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCT                              <  SRR4302537.2896961/94‑1 (MQ=60)

                                                              TCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTG                             <  SRR4302537.2309391/94‑1 (MQ=60)

                                                              TCGCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTG                             <  SRR4302537.2290972/94‑1 (MQ=60)

                                                                GCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAA                           <  SRR4302537.4960761/94‑1 (MQ=60)

                                                                GCCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAA                           >  SRR4302537.4441574/1‑94 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          <  SRR4302537.347993/94‑1 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          <  SRR4302537.1334818/94‑1 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          >  SRR4302537.6457140/1‑94 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          <  SRR4302537.1383273/94‑1 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          >  SRR4302537.2663720/1‑94 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          <  SRR4302537.6275471/94‑1 (MQ=60)

                                                                 CCAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAG                          <  SRR4302537.2063902/94‑1 (MQ=60)

                                                                  CAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGA                         <  SRR4302537.151939/94‑1 (MQ=60)

                                                                  CAGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGA                         >  SRR4302537.2378936/1‑94 (MQ=60)

                                                                   AGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAT                        <  SRR4302537.6072622/94‑1 (MQ=60)

                                                                   AGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAT                        <  SRR4302537.6188553/94‑1 (MQ=60)

                                                                   AGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAT                        >  SRR4302537.5465644/1‑94 (MQ=60)

                                                                   AGATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAT                        >  SRR4302537.4052185/1‑94 (MQ=60)

                                                                    GATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATG                       >  SRR4302537.6251444/1‑94 (MQ=60)

                                                                    GATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATC                       <  SRR4302537.4954699/94‑1 (MQ=60)

                                                                     ATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCG                      <  SRR4302537.5582086/94‑1 (MQ=60)

                                                                     ATGCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCG                      <  SRR4302537.1948393/94‑1 (MQ=60)

                                                                     ATGCTGCAAGAGATGGGCCGCGAACCGACCCCGGAAGAACTGGCTGAACGTATGCTGATCCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCG                      >  SRR4302537.4154141/1‑94 (MQ=60)

                                                                       GCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCC                    <  SRR4302537.4505747/94‑1 (MQ=60)

                                                                       GCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCC                    >  SRR4302537.6340464/1‑94 (MQ=60)

                                                                       GCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCC                    >  SRR4302537.6340463/1‑94 (MQ=60)

                                                                       GCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCA                    >  SRR4302537.5207330/1‑94 (MQ=60)

                                                                       GCTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCACC                    >  SRR4302537.5743711/1‑94 (MQ=60)

                                                                        CTGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCA                   <  SRR4302537.1153865/94‑1 (MQ=60)

                                                                         TGCAAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAA                  <  SRR4302537.1782179/94‑1 (MQ=60)

                                                                            AAGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGA               >  SRR4302537.6115305/1‑94 (MQ=60)

                                                                             AGAGATGGGCCGCGAACCGACGGCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAG              <  SRR4302537.4781035/94‑1 (MQ=60)

                                                                             AGAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAG              >  SRR4302537.1336342/1‑94 (MQ=60)

                                                                              GAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGC             <  SRR4302537.2511550/94‑1 (MQ=60)

                                                                              GAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGC             <  SRR4302537.5182590/94‑1 (MQ=60)

                                                                              GAGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGC             >  SRR4302537.708149/1‑94 (MQ=60)

                                                                               AGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCC            >  SRR4302537.5556947/1‑94 (MQ=60)

                                                                               AGATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCA            >  SRR4302537.3524596/1‑94 (MQ=60)

                                                                                GATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCA           <  SRR4302537.5637849/94‑1 (MQ=60)

                                                                                 ATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAA          >  SRR4302537.1697132/1‑94 (MQ=60)

                                                                                 ATGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAA          <  SRR4302537.909555/94‑1 (MQ=60)

                                                                                  TGGGCCGCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAAT         >  SRR4302537.1344021/1‑94 (MQ=60)

                                                                                        GCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCAT   <  SRR4302537.3706491/94‑1 (MQ=60)

                                                                                        GCGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCAT   <  SRR4302537.3867445/94‑1 (MQ=60)

                                                                                        GAGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCAT   <  SRR4302537.1705192/94‑1 (MQ=60)

                                                                                         CGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATG  <  SRR4302537.3705444/94‑1 (MQ=60)

GCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATG  >  NC_000913/3212026‑3212208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`