Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,311,437 T→C N740S (AAC→AGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,311,4370TC100.0% 996.5 / NA 282N740S (AAC→AGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (157/125);  total (157/125)

Only 100 of 289 total aligned reads displayed.

ACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGT  >  NC_000913/3311347‑3311526
                                                                                          |                                                                                         
aCGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTg                                                                                         <  1:5337870/93‑1 (MQ=255)
 cGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTgg                                                                                        >  1:1742215/1‑93 (MQ=255)
  ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGa                                                                                       <  1:648504/93‑1 (MQ=255)
  ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGa                                                                                       >  1:2927256/1‑93 (MQ=255)
  ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGa                                                                                       >  1:1585833/1‑93 (MQ=255)
   gAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAc                                                                                      >  1:3014611/1‑93 (MQ=255)
    agtagtAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcg                                                                                     >  1:1646756/1‑93 (MQ=255)
      tagtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgcc                                                                                   <  2:4397631/93‑1 (MQ=255)
        gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgccgc                                                                                 <  2:470928/93‑1 (MQ=255)
        gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgccgc                                                                                 >  2:5842005/1‑93 (MQ=255)
         taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCa                                                                                <  1:6111876/93‑1 (MQ=255)
          aaCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAg                                                                               >  1:1942245/1‑93 (MQ=255)
           aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGc                                                                              <  2:6257115/93‑1 (MQ=255)
             gCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCa                                                                            >  2:3146285/1‑93 (MQ=255)
             gCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCa                                                                            <  2:229637/93‑1 (MQ=255)
             gCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCa                                                                            <  2:4597625/93‑1 (MQ=255)
                gATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAggg                                                                         <  2:4819891/93‑1 (MQ=255)
                  tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTg                                                                       <  1:2171644/93‑1 (MQ=255)
                  tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTg                                                                       >  1:4307648/1‑93 (MQ=255)
                    cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGggcggc                                                                     >  1:6361007/1‑93 (MQ=255)
                    cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                     <  1:4940762/93‑1 (MQ=255)
                    cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                     <  1:1125025/93‑1 (MQ=255)
                    cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                     >  2:5209978/1‑93 (MQ=255)
                    cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                     >  2:728536/1‑93 (MQ=255)
                    cAGGCTTNCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                     >  1:4202700/1‑93 (MQ=255)
                     aGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCg                                                                    >  1:4420717/1‑93 (MQ=255)
                     aGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCg                                                                    >  2:6024089/1‑93 (MQ=255)
                      ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGt                                                                   >  2:5348973/1‑93 (MQ=255)
                      ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGt                                                                   >  2:1741210/1‑93 (MQ=255)
                      ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGt                                                                   <  2:6212249/93‑1 (MQ=255)
                       gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTc                                                                  <  1:5965059/93‑1 (MQ=255)
                        cTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCg                                                                 <  2:4195583/93‑1 (MQ=255)
                          ttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGt                                                               >  2:2342189/1‑93 (MQ=255)
                            ccGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGttt                                                             <  2:1443537/93‑1 (MQ=255)
                             cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTc                                                            <  1:4188719/93‑1 (MQ=255)
                                ttCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGt                                                         >  2:3427699/1‑93 (MQ=255)
                                   aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGAt                                                      <  1:1309641/93‑1 (MQ=255)
                                    aTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATa                                                     >  2:4721530/1‑93 (MQ=255)
                                    aTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATa                                                     >  1:3817744/1‑93 (MQ=255)
                                      cACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacc                                                   >  1:1395563/1‑93 (MQ=255)
                                       aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca                                                  >  1:2104486/1‑93 (MQ=255)
                                        cTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                                                 <  1:5748973/93‑1 (MQ=255)
                                         tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                                                <  2:3079329/93‑1 (MQ=255)
                                         tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                                                >  1:5273969/1‑93 (MQ=255)
                                         tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                                                >  2:5778871/1‑93 (MQ=255)
                                         tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                                                >  2:1285282/1‑93 (MQ=255)
                                         tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc                                                <  1:625948/93‑1 (MQ=255)
                                           tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTa                                              <  1:930602/93‑1 (MQ=255)
                                            aCGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc                                             <  1:3102400/93‑1 (MQ=255)
                                                gCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCa                                         >  2:2886113/1‑93 (MQ=255)
                                                  agagGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGa                                       >  2:945204/1‑93 (MQ=255)
                                                  agagGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGa                                       >  1:4743556/1‑93 (MQ=255)
                                                   gagGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGac                                      >  2:2744821/1‑92 (MQ=255)
                                                    agGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAc                                     <  2:4561656/93‑1 (MQ=255)
                                                    agGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAc                                     <  1:3852328/93‑1 (MQ=255)
                                                    agGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAc                                     <  2:3568900/93‑1 (MQ=255)
                                                       cATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCga                                  <  1:3180656/93‑1 (MQ=255)
                                                        aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                 >  2:1374011/1‑93 (MQ=255)
                                                        aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                 <  1:3330959/93‑1 (MQ=255)
                                                        aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                 >  2:4970204/1‑93 (MQ=255)
                                                        aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                 >  1:1510914/1‑93 (MQ=255)
                                                         tCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg                                >  1:3203225/1‑93 (MQ=255)
                                                          cAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatga                               <  2:517444/93‑1 (MQ=255)
                                                           aGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat                              >  2:6396046/1‑93 (MQ=255)
                                                            gCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc                             >  1:2892875/1‑93 (MQ=255)
                                                            gCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc                             <  2:2949445/93‑1 (MQ=255)
                                                            gCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc                             >  1:4788712/1‑93 (MQ=255)
                                                            gCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc                             <  1:1050452/93‑1 (MQ=255)
                                                             cACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCt                            <  2:2021817/93‑1 (MQ=255)
                                                               cgtacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTc                          >  2:2609569/1‑93 (MQ=255)
                                                                 tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                        >  1:5139549/1‑93 (MQ=255)
                                                                 tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                        >  1:5679865/1‑93 (MQ=255)
                                                                 tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                        <  1:2694966/93‑1 (MQ=255)
                                                                 tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                        >  2:4916948/1‑93 (MQ=255)
                                                                 tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                        >  1:727606/1‑93 (MQ=255)
                                                                  acgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACt                       >  2:130687/1‑93 (MQ=255)
                                                                  acgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACt                       <  2:5481816/93‑1 (MQ=255)
                                                                   cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACtt                      >  2:3314756/1‑93 (MQ=255)
                                                                   cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACtt                      <  2:706198/93‑1 (MQ=255)
                                                                   cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACtt                      >  2:1631616/1‑93 (MQ=255)
                                                                    gtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACttt                     >  1:6177288/1‑93 (MQ=255)
                                                                     ttAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTa                    >  2:3795332/1‑93 (MQ=255)
                                                                     ttAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTa                    >  1:83070/1‑93 (MQ=255)
                                                                      tAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTaa                   <  2:489032/93‑1 (MQ=255)
                                                                      tAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTaa                   <  2:2576381/93‑1 (MQ=255)
                                                                       aaaGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAAc                  <  2:1826810/93‑1 (MQ=255)
                                                                        aaGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACt                 >  1:3447858/1‑93 (MQ=255)
                                                                         aGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACtt                <  2:1119266/93‑1 (MQ=255)
                                                                         aGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACtt                <  1:5008354/93‑1 (MQ=255)
                                                                             aaCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc            >  1:5527992/1‑93 (MQ=255)
                                                                             aaCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc            >  1:4612747/1‑93 (MQ=255)
                                                                             aaCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc            >  1:2267302/1‑93 (MQ=255)
                                                                             aaCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc            <  1:2408065/93‑1 (MQ=255)
                                                                              aCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCa           <  2:2117157/93‑1 (MQ=255)
                                                                              aCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCa           >  2:3587637/1‑93 (MQ=255)
                                                                               ccAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAg          <  1:2599191/93‑1 (MQ=255)
                                                                                cAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGt         >  2:391326/1‑93 (MQ=255)
                                                                                 aGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTa        <  1:3959525/93‑1 (MQ=255)
                                                                                     tGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACa    <  1:2160483/93‑1 (MQ=255)
                                                                                      ggCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAg   <  2:4473979/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGT  >  NC_000913/3311347‑3311526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTC  >  NC_000913/3311345‑3311529
                                                                                            |                                                                                            
TGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCT                                                                                             >  SRR4302537.1868437/1‑94 (MQ=60)
 GACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTG                                                                                            >  SRR4302537.3593658/1‑94 (MQ=60)
  ACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGG                                                                                           >  SRR4302537.1754360/1‑94 (MQ=60)
  ACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGG                                                                                           <  SRR4302537.5374465/94‑1 (MQ=60)
   CGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGA                                                                                          >  SRR4302537.2947812/1‑94 (MQ=60)
   CGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGA                                                                                          >  SRR4302537.1596920/1‑94 (MQ=60)
    GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAC                                                                                         <  SRR4302537.652926/94‑1 (MQ=60)
    GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAC                                                                                         >  SRR4302537.2866624/1‑94 (MQ=60)
    GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAC                                                                                         >  SRR4302537.3035628/1‑94 (MQ=60)
     GAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACG                                                                                        >  SRR4302537.1658291/1‑94 (MQ=60)
        TAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCG                                                                                     >  SRR4302537.535777/1‑94 (MQ=60)
           TAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAG                                                                                  >  SRR4302537.1955747/1‑94 (MQ=60)
           TAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAG                                                                                  <  SRR4302537.6153877/94‑1 (MQ=60)
             ACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCC                                                                                <  SRR4302537.5733199/94‑1 (MQ=60)
             ACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCC                                                                                >  SRR4302537.2498107/1‑94 (MQ=60)
              CGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCA                                                                               <  SRR4302537.495983/94‑1 (MQ=60)
               GCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAG                                                                              <  SRR4302537.5832286/94‑1 (MQ=60)
                CAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGG                                                                             <  SRR4302537.4438289/94‑1 (MQ=60)
                CAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGG                                                                             <  SRR4302537.3950869/94‑1 (MQ=60)
                CAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGG                                                                             >  SRR4302537.1567294/1‑94 (MQ=60)
                 AGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGG                                                                            >  SRR4302537.1089863/1‑94 (MQ=60)
                 AGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGG                                                                            >  SRR4302537.2148596/1‑94 (MQ=60)
                 AGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGG                                                                            <  SRR4302537.639372/94‑1 (MQ=60)
                  GATCCAGGGTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGT                                                                           >  SRR4302537.994224/1‑94 (MQ=60)
                  GATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGT                                                                           <  SRR4302537.1397359/94‑1 (MQ=60)
                  GATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGG                                                                           >  SRR4302537.6178617/1‑94 (MQ=60)
                   ATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTG                                                                          >  SRR4302537.4337590/1‑94 (MQ=60)
                   ATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTG                                                                          <  SRR4302537.1629890/94‑1 (MQ=60)
                    TCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGG                                                                         <  SRR4302537.2186951/94‑1 (MQ=60)
                     CCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGC                                                                        >  SRR4302537.6298500/1‑94 (MQ=60)
                     CCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGCGGC                                                                        >  SRR4302537.6404405/1‑94 (MQ=60)
                     CCAGGCTTNCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGC                                                                        >  SRR4302537.4232040/1‑94 (MQ=60)
                      CAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCG                                                                       >  SRR4302537.4272901/1‑94 (MQ=60)
                      CAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCG                                                                       >  SRR4302537.4451237/1‑94 (MQ=60)
                      CAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCG                                                                       <  SRR4302537.1132826/94‑1 (MQ=60)
                      CAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCG                                                                       <  SRR4302537.4974835/94‑1 (MQ=60)
                       AGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGT                                                                      >  SRR4302537.1856979/1‑94 (MQ=60)
                       AGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGT                                                                      >  SRR4302537.4093114/1‑94 (MQ=60)
                         GCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCG                                                                    <  SRR4302537.5263669/94‑1 (MQ=60)
                         GCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCG                                                                    <  SRR4302537.6005998/94‑1 (MQ=60)
                         GCTTACCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCG                                                                    <  SRR4302537.92571/94‑1 (MQ=60)
                          CTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGG                                                                   >  SRR4302537.5800392/1‑94 (MQ=60)
                          CTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGG                                                                   <  SRR4302537.6058997/94‑1 (MQ=60)
                          CTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGG                                                                   <  SRR4302537.2185077/94‑1 (MQ=60)
                          CTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGG                                                                   >  SRR4302537.2261471/1‑94 (MQ=60)
                           TTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGT                                                                  <  SRR4302537.3447646/94‑1 (MQ=60)
                              CCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTC                                                               >  SRR4302537.4596619/1‑94 (MQ=60)
                               CGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCG                                                              <  SRR4302537.4217973/94‑1 (MQ=60)
                               CGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCG                                                              <  SRR4302537.4836707/94‑1 (MQ=60)
                               CGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCG                                                              <  SRR4302537.2972522/94‑1 (MQ=60)
                                GCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGG                                                             <  SRR4302537.2820732/94‑1 (MQ=60)
                                GCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGG                                                             >  SRR4302537.3218824/1‑94 (MQ=60)
                                 CTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGT                                                            <  SRR4302537.954233/94‑1 (MQ=60)
                                 CTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGT                                                            <  SRR4302537.820857/94‑1 (MQ=60)
                                  TTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTG                                                           >  SRR4302537.5780929/1‑94 (MQ=60)
                                   TCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGA                                                          >  SRR4302537.2652061/1‑94 (MQ=60)
                                    CAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGAT                                                         >  SRR4302537.2052180/1‑94 (MQ=60)
                                     AATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATA                                                        <  SRR4302537.1318758/94‑1 (MQ=60)
                                     AATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATA                                                        >  SRR4302537.3844516/1‑94 (MQ=60)
                                      ATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATAC                                                       >  SRR4302537.3286075/1‑94 (MQ=60)
                                       TCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACC                                                      >  SRR4302537.1405294/1‑94 (MQ=60)
                                        CACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCA                                                     <  SRR4302537.2328787/94‑1 (MQ=60)
                                        CACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCA                                                     >  SRR4302537.2119320/1‑94 (MQ=60)
                                        CACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCA                                                     >  SRR4302537.3229621/1‑94 (MQ=60)
                                        CACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCA                                                     <  SRR4302537.4677717/94‑1 (MQ=60)
                                        CACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCA                                                     >  SRR4302537.6104702/1‑94 (MQ=60)
                                          CTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACC                                                   <  SRR4302537.5788349/94‑1 (MQ=60)
                                          CTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACC                                                   <  SRR4302537.3874685/94‑1 (MQ=60)
                                          CTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACC                                                   >  SRR4302537.5310261/1‑94 (MQ=60)
                                           TTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCT                                                  <  SRR4302537.630214/94‑1 (MQ=60)
                                           TTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCT                                                  >  SRR4302537.2934177/1‑94 (MQ=60)
                                             TACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAC                                                <  SRR4302537.937015/94‑1 (MQ=60)
                                              ACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACG                                               <  SRR4302537.2384911/94‑1 (MQ=60)
                                              ACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACG                                               <  SRR4302537.3124062/94‑1 (MQ=60)
                                                 TGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCA                                            <  SRR4302537.5012877/94‑1 (MQ=60)
                                                   CAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGA                                          >  SRR4302537.4776336/1‑94 (MQ=60)
                                                   CAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGA                                          >  SRR4302537.5353133/1‑94 (MQ=60)
                                                   CAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGAGTCGGTTTCGGTGATACCACCTACGCCAGA                                          >  SRR4302537.4225882/1‑94 (MQ=60)
                                                    AGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGTAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAAGCCAGAA                                         <  SRR4302537.4574630/94‑1 (MQ=60)
                                                    AGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTT‑GGTGATACCACCTACGCCAGAAC                                        >  SRR4302537.269644/1‑94 (MQ=60)
                                                     GAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAC                                        <  SRR4302537.6218546/94‑1 (MQ=60)
                                                     GAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAC                                        <  SRR4302537.5528910/94‑1 (MQ=60)
                                                      AGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACC                                       <  SRR4302537.3879301/94‑1 (MQ=60)
                                                      AGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACC                                       <  SRR4302537.2011419/94‑1 (MQ=60)
                                                      AGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACC                                       >  SRR4302537.4724997/1‑94 (MQ=60)
                                                       GGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCG                                      >  SRR4302537.4021499/1‑94 (MQ=60)
                                                       GGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCG                                      <  SRR4302537.2920439/94‑1 (MQ=60)
                                                       GGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCG                                      >  SRR4302537.3220288/1‑94 (MQ=60)
                                                       GGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCG                                      >  SRR4302537.1215975/1‑94 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    <  SRR4302537.2466945/94‑1 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    <  SRR4302537.800434/94‑1 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    >  SRR4302537.2183197/1‑94 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    <  SRR4302537.3203011/94‑1 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    >  SRR4302537.143342/1‑94 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    <  SRR4302537.3364683/94‑1 (MQ=60)
                                                         CATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGAT                                    >  SRR4302537.1521474/1‑94 (MQ=60)
                                                          ATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATG                                   >  SRR4302537.1539232/1‑94 (MQ=60)
                                                          ATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATG                                   >  SRR4302537.3225743/1‑94 (MQ=60)
                                                          ATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATG                                   <  SRR4302537.3354526/94‑1 (MQ=60)
                                                          ATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATG                                   >  SRR4302537.2281496/1‑94 (MQ=60)
                                                            CAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGAT                                 >  SRR4302537.5120145/1‑94 (MQ=60)
                                                            CAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGAT                                 >  SRR4302537.3849333/1‑94 (MQ=60)
                                                             AGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC                                <  SRR4302537.5573096/94‑1 (MQ=60)
                                                             AGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC                                >  SRR4302537.4821766/1‑94 (MQ=60)
                                                             AGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC                                >  SRR4302537.2913184/1‑94 (MQ=60)
                                                              GCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCT                               <  SRR4302537.1057696/94‑1 (MQ=60)
                                                                ACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTC                             <  SRR4302537.3756745/94‑1 (MQ=60)
                                                                 CGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCA                            <  SRR4302537.2991619/94‑1 (MQ=60)
                                                                  GTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAC                           >  SRR4302537.5718720/1‑94 (MQ=60)
                                                                  GTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAC                           >  SRR4302537.732574/1‑94 (MQ=60)
                                                                  GTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAC                           >  SRR4302537.1812223/1‑94 (MQ=60)
                                                                  GTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAC                           >  SRR4302537.5174963/1‑94 (MQ=60)
                                                                  GTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAC                           >  SRR4302537.5077957/1‑94 (MQ=60)
                                                                   TACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACT                          <  SRR4302537.2713798/94‑1 (MQ=60)
                                                                     CGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTT                        <  SRR4302537.1748985/94‑1 (MQ=60)
                                                                     CGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTT                        >  SRR4302537.3188849/1‑94 (MQ=60)
                                                                     CGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTT                        >  SRR4302537.6219830/1‑94 (MQ=60)
                                                                     CGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTT                        >  SRR4302537.1481811/1‑94 (MQ=60)
                                                                     CGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTT                        >  SRR4302537.1674880/1‑94 (MQ=60)
                                                                      GTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTA                       >  SRR4302537.83679/1‑94 (MQ=60)
                                                                       TTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAA                      <  SRR4302537.4290740/94‑1 (MQ=60)
                                                                        TGAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAAC                     <  SRR4302537.439322/94‑1 (MQ=60)
                                                                        TAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAAC                     >  SRR4302537.6412777/1‑94 (MQ=60)
                                                                         AAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACT                    >  SRR4302537.3472247/1‑94 (MQ=60)
                                                                         AAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACT                    <  SRR4302537.6474838/94‑1 (MQ=60)
                                                                          AAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTT                   <  SRR4302537.1309095/94‑1 (MQ=60)
                                                                           AGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTC                  >  SRR4302537.4981485/1‑94 (MQ=60)
                                                                           AGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTC                  <  SRR4302537.5680417/94‑1 (MQ=60)
                                                                           AGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTC                  <  SRR4302537.71795/94‑1 (MQ=60)
                                                                           AGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTC                  <  SRR4302537.5042852/94‑1 (MQ=60)
                                                                              CAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTC               >  SRR4302537.4644685/1‑94 (MQ=60)
                                                                              CAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTC               >  SRR4302537.2283312/1‑94 (MQ=60)
                                                                              CAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTC               >  SRR4302537.5565727/1‑94 (MQ=60)
                                                                               AACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCA              <  SRR4302537.2425015/94‑1 (MQ=60)
                                                                               AACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCA              >  SRR4302537.3961487/1‑94 (MQ=60)
                                                                                ACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAG             >  SRR4302537.1363897/1‑94 (MQ=60)
                                                                                 CCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGT            >  SRR4302537.67152/1‑94 (MQ=60)
                                                                                 CCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGT            <  SRR4302537.2617369/94‑1 (MQ=60)
                                                                                 CCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGT            <  SRR4302537.8772/94‑1 (MQ=60)
                                                                                  CAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTA           >  SRR4302537.6292639/1‑94 (MQ=60)
                                                                                   AGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAG          <  SRR4302537.3987229/94‑1 (MQ=60)
                                                                                   AGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAG          <  SRR4302537.5529069/94‑1 (MQ=60)
                                                                                     GATGGCGCTGGACGCCGCAGCTAGCGTGGCGTCGGTTTCGGTTATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGAC        <  SRR4302537.4790237/94‑1 (MQ=60)
                                                                                       TGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAG      >  SRR4302537.3050695/1‑94 (MQ=60)
                                                                                       TGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAG      >  SRR4302537.3185786/1‑94 (MQ=60)
                                                                                       TGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAG      >  SRR4302537.4781725/1‑94 (MQ=60)
                                                                                       TGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAG      <  SRR4302537.2175712/94‑1 (MQ=60)
                                                                                        GGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGT     <  SRR4302537.1637688/94‑1 (MQ=60)
                                                                                          CGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTT   >  SRR4302537.1250511/1‑94 (MQ=60)
                                                                                          CGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTT   >  SRR4302537.2175716/1‑94 (MQ=60)
                                                                                           GCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTC  <  SRR4302537.977766/94‑1 (MQ=60)
                                                                                            |                                                                                            
TGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTC  >  NC_000913/3311345‑3311529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑