Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,877	2 bp→CG	coding (586‑587/2022 nt)	rep →	rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,877	0	G	C	100.0%	809.2 / NA	229	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (129/100); total (129/100)
*	NC_000913	3,958,878	0	C	G	100.0%	805.8 / NA	229	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (129/100); total (129/100)

Only 100 of 234 total aligned reads displayed.

TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958790‑3958964

||

tGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAAt                                                                                    >  1:1112116/1‑93 (MQ=255)

 gTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATg                                                                                   >  1:2419274/1‑93 (MQ=255)

     tGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaaga                                                                               >  1:2942881/1‑93 (MQ=255)

        gCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGaagaagg                                                                            >  2:1145837/1‑92 (MQ=255)

        gCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGt                                                                            >  2:2267297/1‑93 (MQ=255)

        gCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGt                                                                            >  2:1257371/1‑93 (MQ=255)

        gCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGt                                                                            <  1:1717348/93‑1 (MQ=255)

         cTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTc                                                                           <  2:4427554/93‑1 (MQ=255)

          tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTcc                                                                          >  1:5811073/1‑93 (MQ=255)

           gTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGAAATGAAGAAGTCCg                                                                         >  1:6344004/1‑93 (MQ=255)

            tATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGc                                                                        >  1:1587644/1‑93 (MQ=255)

            tATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGc                                                                        <  1:5014831/93‑1 (MQ=255)

              tgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCaa                                                                      <  2:2770967/93‑1 (MQ=255)

              tgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCaa                                                                      <  2:7001/93‑1 (MQ=255)

               gatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAg                                                                     >  2:3307305/1‑93 (MQ=255)

                atgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgc                                                                    <  1:1166296/93‑1 (MQ=255)

                atgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgc                                                                    >  1:3889743/1‑93 (MQ=255)

                  gCACACCTGAAAGCCTGTAACTTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  <  2:921541/93‑1 (MQ=255)

                  gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  >  2:437181/1‑93 (MQ=255)

                  gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  <  2:577960/93‑1 (MQ=255)

                  gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  >  2:3155322/1‑93 (MQ=255)

                    ccacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                <  1:6211350/92‑1 (MQ=255)

                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                <  2:4277446/93‑1 (MQ=255)

                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                >  1:5883834/1‑93 (MQ=255)

                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                >  1:760604/1‑93 (MQ=255)

                     cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  1:5624689/1‑93 (MQ=255)

                     cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  2:1581633/1‑93 (MQ=255)

                      acCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGc                                                              <  2:4028376/93‑1 (MQ=255)

                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  1:4607808/93‑1 (MQ=255)

                         tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           >  1:2743592/1‑93 (MQ=255)

                          gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          <  1:483843/93‑1 (MQ=255)

                          gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  1:5655868/1‑93 (MQ=255)

                          gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  1:3028377/1‑93 (MQ=255)

                           aaaGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAc                                                         <  1:492325/93‑1 (MQ=255)

                              gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      <  1:6413527/93‑1 (MQ=255)

                              gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      <  2:1703486/93‑1 (MQ=255)

                              gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      <  2:1085449/93‑1 (MQ=255)

                               ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:2942324/93‑1 (MQ=255)

                               ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:4114431/93‑1 (MQ=255)

                               ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:3605008/93‑1 (MQ=255)

                                 tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   >  2:3049193/1‑93 (MQ=255)

                                 tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   >  2:4539131/1‑93 (MQ=255)

                                   tAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCg                                                 >  1:4251158/1‑93 (MQ=255)

                                   tAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCg                                                 >  2:5656177/1‑93 (MQ=255)

                                   tAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCg                                                 <  1:5208313/93‑1 (MQ=255)

                                     aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               >  1:4206969/1‑93 (MQ=255)

                                      cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              >  1:4081150/1‑93 (MQ=255)

                                      cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              <  2:247687/93‑1 (MQ=255)

                                       gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             <  2:2168912/93‑1 (MQ=255)

                                       gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             >  2:4329214/1‑93 (MQ=255)

                                           tcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         >  2:5063211/1‑93 (MQ=255)

                                             gACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgctg                                       <  2:511299/93‑1 (MQ=255)

                                              aCTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtgg                                      <  2:1778618/93‑1 (MQ=255)

                                               cTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggt                                     <  2:1812047/93‑1 (MQ=255)

                                                ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    >  1:2319660/1‑93 (MQ=255)

                                                ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    <  1:208861/93‑1 (MQ=255)

                                                  cGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGa                                  <  1:1402361/93‑1 (MQ=255)

                                                   gatgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAt                                 >  2:4794013/1‑93 (MQ=255)

                                                   gatgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAt                                 >  1:192567/1‑93 (MQ=255)

                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTTCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                >  2:4762208/1‑93 (MQ=255)

                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                >  1:600311/1‑93 (MQ=255)

                                                     tgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATgg                               >  2:3679163/1‑92 (MQ=255)

                                                     tgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGa                               >  2:6062227/1‑93 (MQ=255)

                                                     tgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGa                               >  2:6360831/1‑93 (MQ=255)

                                                     tgatCTGAATTTATTGCCGTCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGa                               >  2:6285722/1‑93 (MQ=255)

                                                      gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGCAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:3383742/1‑93 (MQ=255)

                                                      gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              <  1:4817993/93‑1 (MQ=255)

                                                      gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:4700511/1‑93 (MQ=255)

                                                       atCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGt                             >  2:154163/1‑93 (MQ=255)

                                                       atCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT‑GTGGATGAGTa                            <  2:6415063/93‑1 (MQ=255)

                                                        tCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTa                            >  2:233579/1‑93 (MQ=255)

                                                         cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           <  2:755200/93‑1 (MQ=255)

                                                         cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           >  2:3421693/1‑93 (MQ=255)

                                                          tGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATc                          <  1:1433818/93‑1 (MQ=255)

                                                           gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         <  1:5080203/93‑1 (MQ=255)

                                                           gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  1:906272/1‑93 (MQ=255)

                                                           gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  1:319712/1‑93 (MQ=255)

                                                           gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         >  2:2523102/1‑93 (MQ=255)

                                                            aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        >  1:4372002/1‑93 (MQ=255)

                                                             ttttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAgg                       >  1:2576692/1‑93 (MQ=255)

                                                              tttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGa                      >  1:3131896/1‑93 (MQ=255)

                                                                 aTTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacac                   <  1:1870117/93‑1 (MQ=255)

                                                                   tGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacacca                 <  2:3731785/93‑1 (MQ=255)

                                                                     ccGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaac               <  1:1015806/93‑1 (MQ=255)

                                                                     ccGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaac               <  1:711020/93‑1 (MQ=255)

                                                                       gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  1:3321405/1‑93 (MQ=255)

                                                                        aCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacc            >  1:5427578/1‑93 (MQ=255)

                                                                         cGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacca           >  2:3217429/1‑93 (MQ=255)

                                                                          gTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccag          <  1:2996571/93‑1 (MQ=255)

                                                                            tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        <  1:4778171/93‑1 (MQ=255)

                                                                            tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        <  1:3500641/93‑1 (MQ=255)

                                                                             gctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcca       >  1:5596806/1‑93 (MQ=255)

                                                                             gctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcca       <  1:5261515/93‑1 (MQ=255)

                                                                              ctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagccag      <  1:2869513/93‑1 (MQ=255)

                                                                              ctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagccag      <  1:448402/93‑1 (MQ=255)

                                                                               tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     >  1:3646753/1‑93 (MQ=255)

                                                                               tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     >  1:3120109/1‑93 (MQ=255)

                                                                                gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    >  2:5015974/1‑93 (MQ=255)

                                                                                gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    >  2:6201572/1‑93 (MQ=255)

                                                                                 ctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAt   <  2:556209/93‑1 (MQ=255)

||

TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958790‑3958964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                           <  SRR4302537.4562531/94‑1 (MQ=60)

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                           >  SRR4302537.4986086/1‑94 (MQ=60)

     TTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAAT                                                                                      >  SRR4302537.1119796/1‑94 (MQ=60)

      TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATG                                                                                     >  SRR4302537.2436325/1‑94 (MQ=60)

       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                    <  SRR4302537.4463856/94‑1 (MQ=60)

       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                    >  SRR4302537.1589596/1‑94 (MQ=60)

        TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTTCCGACGTTGCTGCTGCAACGCAATGAA                                                                                   >  SRR4302537.2807484/1‑94 (MQ=60)

          ATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGA                                                                                 >  SRR4302537.2963542/1‑94 (MQ=60)

           TGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAA                                                                                <  SRR4302537.3268537/94‑1 (MQ=60)

           TGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAA                                                                                <  SRR4302537.847948/94‑1 (MQ=60)

            GGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAG                                                                               <  SRR4302537.559091/94‑1 (MQ=60)

             GTCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                              <  SRR4302537.2616957/94‑1 (MQ=60)

             GGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                              <  SRR4302537.5109122/94‑1 (MQ=60)

              GCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTC                                                                             <  SRR4302537.1729334/94‑1 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            >  SRR4302537.4795267/1‑94 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            >  SRR4302537.5850913/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCG                                                                           >  SRR4302537.3703341/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCG                                                                           >  SRR4302537.2368659/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCG                                                                           >  SRR4302537.946047/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGAAATGAAGAAGTCCG                                                                           >  SRR4302537.6387341/1‑94 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTCGCTGCTGCAACGCAATGAAGAAGTCCG                                                                           >  SRR4302537.2944053/1‑94 (MQ=60)

                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                          >  SRR4302537.1598741/1‑94 (MQ=60)

                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                          >  SRR4302537.410513/1‑94 (MQ=60)

                  TATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCA                                                                         <  SRR4302537.5049372/94‑1 (MQ=60)

                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                        <  SRR4302537.5690727/94‑1 (MQ=60)

                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGATGCTGCAACGCAATGAAGAAGTCCGCAA                                                                        >  SRR4302537.5777050/1‑94 (MQ=60)

                    TGATGCACGCCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAG                                                                       <  SRR4302537.6205941/94‑1 (MQ=60)

                     GATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGC                                                                      >  SRR4302537.3916949/1‑94 (MQ=60)

                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                     <  SRR4302537.1174405/94‑1 (MQ=60)

                        GCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCT                                                                   >  SRR4302537.4517935/1‑94 (MQ=60)

                        GCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCT                                                                   <  SRR4302537.1667231/94‑1 (MQ=60)

                        GCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCT                                                                   <  SRR4302537.2019407/94‑1 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302537.765803/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302537.5192136/1‑94 (MQ=60)

                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                  >  SRR4302537.5924205/1‑94 (MQ=60)

                          CCACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 <  SRR4302537.6254138/94‑1 (MQ=60)

                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 <  SRR4302537.6448181/94‑1 (MQ=60)

                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                 >  SRR4302537.5663153/1‑94 (MQ=60)

                            ACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCA                                                               <  SRR4302537.3110529/94‑1 (MQ=60)

                            ACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCA                                                               >  SRR4302537.847422/1‑94 (MQ=60)

                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                              <  SRR4302537.4639714/94‑1 (MQ=60)

                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                              >  SRR4302537.2912979/1‑94 (MQ=60)

                              CTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                             >  SRR4302537.2762731/1‑94 (MQ=60)

                               TGAAAGCCTGTAACGTTCTCGACTTTGATTTTCTGATTTTATTGCCGACGTTTCTGCTGCAACGCAATTAAGAAGTCTGCAATCGCTGGCAGTA                                                            >  SRR4302537.6511208/1‑94 (MQ=48)

                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                            >  SRR4302537.5694570/1‑94 (MQ=60)

                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                            >  SRR4302537.3049492/1‑94 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           <  SRR4302537.6396818/94‑1 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           >  SRR4302537.2583893/1‑94 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           <  SRR4302537.487158/94‑1 (MQ=60)

                                 AAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACA                                                          <  SRR4302537.495714/94‑1 (MQ=60)

                                 AAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACA                                                          <  SRR4302537.5272926/94‑1 (MQ=60)

                                  AAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAA                                                         <  SRR4302537.3560043/94‑1 (MQ=60)

                                   AGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAA                                                        >  SRR4302537.4132817/1‑94 (MQ=60)

                                    GCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                       <  SRR4302537.6457152/94‑1 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAATAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      >  SRR4302537.6260082/1‑94 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      >  SRR4302537.315269/1‑94 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      >  SRR4302537.2511119/1‑94 (MQ=60)

                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGCAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                    >  SRR4302537.4504345/1‑94 (MQ=60)

                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                    <  SRR4302537.1090311/94‑1 (MQ=60)

                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                   >  SRR4302537.4280703/1‑94 (MQ=60)

                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGC                                                  <  SRR4302537.5244130/94‑1 (MQ=60)

                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATTAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                 >  SRR4302537.4946525/1‑94 (MQ=60)

                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                 >  SRR4302537.4236325/1‑94 (MQ=60)

                                           ACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTA                                                >  SRR4302537.4109639/1‑94 (MQ=60)

                                            CGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAT                                               >  SRR4302537.1391812/1‑94 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              >  SRR4302537.4824905/1‑94 (MQ=60)

                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                          <  SRR4302537.1909671/94‑1 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        <  SRR4302537.112968/94‑1 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        >  SRR4302537.1418490/1‑94 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        <  SRR4302537.1776243/94‑1 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        >  SRR4302537.1709070/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302537.2336041/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302537.1960/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTACCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      <  SRR4302537.5888117/94‑1 (MQ=60)

                                                      TTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGG                                     <  SRR4302537.4255631/94‑1 (MQ=60)

                                                      TTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGG                                     <  SRR4302537.210361/94‑1 (MQ=60)

                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAT                                   <  SRR4302537.729239/94‑1 (MQ=60)

                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAT                                   >  SRR4302537.4362648/1‑94 (MQ=60)

                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAT                                   <  SRR4302537.1412129/94‑1 (MQ=60)

                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAT                                   >  SRR4302537.193932/1‑94 (MQ=60)

                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  >  SRR4302537.604417/1‑94 (MQ=60)

                                                         AATGATCTGATGTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGCCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  <  SRR4302537.3653992/94‑1 (MQ=60)

                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                 >  SRR4302537.6120694/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGCAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302537.3407715/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                <  SRR4302537.4855301/94‑1 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302537.4733028/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                <  SRR4302537.5341437/94‑1 (MQ=60)

                                                            GATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                               <  SRR4302537.4851261/94‑1 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302537.130351/94‑1 (MQ=60)

                                                              TATGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGGAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302537.3309456/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           >  SRR4302537.321927/1‑94 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           <  SRR4302537.1443792/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           <  SRR4302537.2167117/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           <  SRR4302537.5931239/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           >  SRR4302537.912532/1‑94 (MQ=60)

                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                          <  SRR4302537.5115173/94‑1 (MQ=60)

                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                          >  SRR4302537.4402208/1‑94 (MQ=60)

                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGTAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                         >  SRR4302537.3167368/1‑94 (MQ=60)

                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                         >  SRR4302537.4799570/1‑94 (MQ=60)

                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                         >  SRR4302537.2594732/1‑94 (MQ=60)

                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                        >  SRR4302537.3153868/1‑94 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      >  SRR4302537.3036280/1‑94 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGATGGTGGATGAGTATCAGGACA                      <  SRR4302537.5223696/94‑1 (MQ=60)

                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                    <  SRR4302537.1883133/94‑1 (MQ=60)

                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                    >  SRR4302537.338639/1‑94 (MQ=60)

                                                                         TGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGAGGATGAGTATCAGGACACCAA                  >  SRR4302537.5814192/1‑94 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                <  SRR4302537.1022802/94‑1 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                <  SRR4302537.715886/94‑1 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                >  SRR4302537.5016966/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               <  SRR4302537.2966768/94‑1 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302537.3344901/1‑94 (MQ=60)

                                                                             GACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACC              >  SRR4302537.5464585/1‑94 (MQ=60)

                                                                              ACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCA             >  SRR4302537.5656881/1‑94 (MQ=60)

                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC           >  SRR4302537.5539213/1‑94 (MQ=60)

                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC           <  SRR4302537.3017465/94‑1 (MQ=60)

                                                                                 TTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCC          >  SRR4302537.3076754/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         <  SRR4302537.3525408/94‑1 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         >  SRR4302537.5635073/1‑94 (MQ=60)

                                                                                  TGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCA         <  SRR4302537.4811158/94‑1 (MQ=60)

                                                                                   GCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAG        <  SRR4302537.5297715/94‑1 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGTGTATCAGGACACCAACACCAGacaga       >  SRR4302537.6509681/1‑89 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT       >  SRR4302537.3672422/1‑94 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT       <  SRR4302537.451511/94‑1 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT       >  SRR4302537.3141919/1‑94 (MQ=60)

                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT       <  SRR4302537.2889653/94‑1 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      >  SRR4302537.4872133/1‑94 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      >  SRR4302537.4682732/1‑94 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      <  SRR4302537.3436722/94‑1 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      >  SRR4302537.4131787/1‑94 (MQ=60)

                                                                                         GCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  <  SRR4302537.2723071/94‑1 (MQ=60)

                                                                                         GCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  <  SRR4302537.5836542/94‑1 (MQ=60)

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`