Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,588	C→G	R86T (AGG→ACG)	gppA ←	guanosine pentaphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,588	0	C	G	100.0%	977.7 / NA	280	R86T (AGG→ACG)	gppA	guanosine pentaphosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (158/122); total (158/122)

Only 100 of 284 total aligned reads displayed.

TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT  >  NC_000913/3961498‑3961676

    ctTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCg                                                                                    >  2:5301943/1‑93 (MQ=255)

     ttCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGt                                                                                   <  1:1870833/93‑1 (MQ=255)

      tCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTa                                                                                  >  1:2917955/1‑93 (MQ=255)

       cACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAg                                                                                 >  2:5410560/1‑93 (MQ=255)

        aCCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGc                                                                                <  2:4980809/93‑1 (MQ=255)

         ccGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCg                                                                               >  1:3822548/1‑93 (MQ=255)

          cGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGa                                                                              >  2:2403387/1‑93 (MQ=255)

           gCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAc                                                                             >  1:6060904/1‑93 (MQ=255)

             tGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa                                                                           >  2:688175/1‑93 (MQ=255)

             tGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa                                                                           >  1:2797314/1‑93 (MQ=255)

             tGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACaa                                                                           <  1:2666972/93‑1 (MQ=255)

              gATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAc                                                                          >  1:5780317/1‑93 (MQ=255)

              gATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAc                                                                          >  1:4808976/1‑93 (MQ=255)

               aTCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAATGCGTAACGTCGCCGTAGCGACAAcg                                                                         >  1:1025574/1‑93 (MQ=255)

               aTCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcg                                                                         <  1:2075648/93‑1 (MQ=255)

                tCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAcgc                                                                        <  2:3936733/93‑1 (MQ=255)

                  aCCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGa                                                                      >  2:5067129/1‑93 (MQ=255)

                   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGaa                                                                     >  2:1133925/1‑93 (MQ=255)

                   ccTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGaa                                                                     >  1:1341574/1‑93 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAt                                                                    <  1:5509956/93‑1 (MQ=255)

                    cTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAt                                                                    >  2:4501648/1‑93 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt                                                                   <  1:2490594/93‑1 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt                                                                   >  2:3350531/1‑93 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt                                                                   <  1:1730475/93‑1 (MQ=255)

                     tGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCCTAGCGACAACGCGAAtt                                                                   >  2:3685508/1‑93 (MQ=255)

                     cgTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAAtt                                                                   <  1:1979048/92‑1 (MQ=255)

                       tACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTg                                                                 <  2:6154506/93‑1 (MQ=255)

                       tACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTg                                                                 >  2:3782268/1‑93 (MQ=255)

                          cGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCga                                                              <  2:359935/93‑1 (MQ=255)

                            gACAACCGAGAATTTCCTGCTCTTTGGCAATAAAATCCCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagg                                                            <  1:1187748/93‑1 (MQ=255)

                            gACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagg                                                            >  1:220294/1‑93 (MQ=255)

                            gACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgagg                                                            >  2:4675226/1‑93 (MQ=255)

                             aCAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgaggg                                                           <  2:2289787/93‑1 (MQ=255)

                             aCAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgaggg                                                           >  2:1604415/1‑93 (MQ=255)

                              cAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCgaggga                                                          <  2:5161225/93‑1 (MQ=255)

                                 ccGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGggagga                                                       >  2:609769/1‑93 (MQ=255)

                                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGaa                                                      >  2:4796757/1‑93 (MQ=255)

                                  cGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGaa                                                      >  2:1423609/1‑93 (MQ=255)

                                   gagaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAat                                                     >  1:5167627/1‑93 (MQ=255)

                                    agaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAata                                                    >  2:3098818/1‑93 (MQ=255)

                                     gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAATGCGTAACGTCTCCGTAACGACAACGCGAATTTGCGAGGGAGGAatat                                                   >  2:4113003/1‑93 (MQ=255)

                                     gaATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAatat                                                   >  2:3229071/1‑93 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCt                                                 <  1:3311171/93‑1 (MQ=255)

                                       aTTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCt                                                 <  1:892160/93‑1 (MQ=255)

                                        tttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCtt                                                <  1:4780627/93‑1 (MQ=255)

                                         ttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTg                                               <  1:4929712/93‑1 (MQ=255)

                                         ttCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTg                                               <  2:1697022/93‑1 (MQ=255)

                                          tCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGc                                              >  1:4734585/1‑93 (MQ=255)

                                          tCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGc                                              <  1:4297704/93‑1 (MQ=255)

                                           ccTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCa                                             <  1:3055335/93‑1 (MQ=255)

                                            cTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAg                                            >  2:2536597/1‑93 (MQ=255)

                                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGa                                           >  2:2711804/1‑93 (MQ=255)

                                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGa                                           <  2:5744601/93‑1 (MQ=255)

                                             tGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGa                                           >  1:4565514/1‑93 (MQ=255)

                                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                        >  2:2865030/1‑93 (MQ=255)

                                                gcTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGt                                        <  1:6342202/93‑1 (MQ=255)

                                                 cTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGtt                                       >  2:2794622/1‑93 (MQ=255)

                                                  tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc                                      >  2:1898013/1‑93 (MQ=255)

                                                  tttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTc                                      >  1:3648126/1‑93 (MQ=255)

                                                   ttGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCa                                     >  2:1710497/1‑93 (MQ=255)

                                                    tGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAg                                    >  2:434572/1‑93 (MQ=255)

                                                        aaTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCaaa                                <  1:2855096/93‑1 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                               >  2:2938683/1‑93 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                               <  1:877405/93‑1 (MQ=255)

                                                         aTAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAc                               >  1:56495/1‑93 (MQ=255)

                                                          tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                              >  1:648122/1‑93 (MQ=255)

                                                          tAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACa                              <  1:2397583/93‑1 (MQ=255)

                                                           aaaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAg                             <  1:4014103/93‑1 (MQ=255)

                                                            aaaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAgg                            <  2:34054/93‑1 (MQ=255)

                                                             aaTCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGgc                           <  1:4369305/93‑1 (MQ=255)

                                                                cACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCa                        >  2:4285456/1‑93 (MQ=255)

                                                                 aCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaa                       >  1:3539833/1‑93 (MQ=255)

                                                                 aCCCGCATTGACGGCAAGGCGTAACGTCGCCGGAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGTGCaa                       >  2:2165411/1‑93 (MQ=255)

                                                                  cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCTACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                      >  1:3500531/1‑93 (MQ=255)

                                                                  cccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCaaa                      >  2:2481699/1‑93 (MQ=255)

                                                                   ccGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAc                     >  1:2277116/1‑93 (MQ=255)

                                                                    cGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACa                    <  2:1932414/93‑1 (MQ=255)

                                                                    cGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACa                    <  1:4847542/93‑1 (MQ=255)

                                                                     gCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAt                   >  2:2233933/1‑93 (MQ=255)

                                                                      cATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAtt                  >  1:6230087/1‑93 (MQ=255)

                                                                       aTTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTg                 <  1:5078164/93‑1 (MQ=255)

                                                                        ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                >  2:4210333/1‑93 (MQ=255)

                                                                        ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                >  1:1809539/1‑93 (MQ=255)

                                                                        ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                >  1:3119351/1‑93 (MQ=255)

                                                                        ttGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGc                >  1:1598821/1‑93 (MQ=255)

                                                                         tGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGcc               <  2:458496/93‑1 (MQ=255)

                                                                           aCGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCaa             <  2:5995257/93‑1 (MQ=255)

                                                                            cGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAc            <  1:106415/93‑1 (MQ=255)

                                                                             ggCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAcc           >  1:3239138/1‑93 (MQ=255)

                                                                              gCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcg          <  2:5864469/93‑1 (MQ=255)

                                                                               cAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcgc         <  1:5921708/93‑1 (MQ=255)

                                                                               cAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcgc         >  1:1519099/1‑93 (MQ=255)

                                                                               cAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTACAGCAAACAGGCGCAAACATTGCCAACcgc         <  1:4633389/93‑1 (MQ=255)

                                                                                aaGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACcgcg        >  1:2365465/1‑93 (MQ=255)

                                                                                 aGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCgcgc       >  2:3318402/1‑93 (MQ=255)

                                                                                  ggCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCt      >  1:4159268/1‑93 (MQ=255)

                                                                                    cGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTcc    <  2:5640393/93‑1 (MQ=255)

                                                                                     gTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa   <  2:1308121/93‑1 (MQ=255)

                                                                                     gTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCa   >  1:3890422/1‑93 (MQ=255)

                                                                                      tAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAt  >  2:782922/1‑93 (MQ=255)

TCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT  >  NC_000913/3961498‑3961676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  >  NC_000913/3961495‑3961681

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACG                                                                                               >  SRR4302537.6036429/1‑94 (MQ=60)

 CTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGT                                                                                              >  SRR4302537.5158270/1‑94 (MQ=60)

 CTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGT                                                                                              >  SRR4302537.4681683/1‑94 (MQ=60)

 CTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGT                                                                                              <  SRR4302537.3016938/94‑1 (MQ=60)

 CTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGT                                                                                              >  SRR4302537.3457253/1‑94 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             <  SRR4302537.665027/94‑1 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             <  SRR4302537.2896872/94‑1 (MQ=60)

  TTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTC                                                                                             >  SRR4302537.5682839/1‑94 (MQ=60)

     CTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCC                                                                                          >  SRR4302537.685631/1‑94 (MQ=60)

     CTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCC                                                                                          <  SRR4302537.5898301/94‑1 (MQ=60)

        TTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTA                                                                                       <  SRR4302537.1883852/94‑1 (MQ=60)

        TTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTA                                                                                       >  SRR4302537.2938457/1‑94 (MQ=60)

        TTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTA                                                                                       >  SRR4302537.933643/1‑94 (MQ=60)

         TCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAG                                                                                      <  SRR4302537.4357390/94‑1 (MQ=60)

         TCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAG                                                                                      <  SRR4302537.51935/94‑1 (MQ=60)

          CACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGC                                                                                     >  SRR4302537.1080741/1‑94 (MQ=60)

           ACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCG                                                                                    >  SRR4302537.3849352/1‑94 (MQ=60)

           ACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCG                                                                                    <  SRR4302537.4848508/94‑1 (MQ=60)

           ACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCG                                                                                    >  SRR4302537.5157822/1‑94 (MQ=60)

             CGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGAC                                                                                  >  SRR4302537.6102546/1‑94 (MQ=60)

               CTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAA                                                                                >  SRR4302537.1549044/1‑94 (MQ=60)

               CTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAA                                                                                >  SRR4302537.2816875/1‑94 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGTAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               >  SRR4302537.6326950/1‑94 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               >  SRR4302537.5819931/1‑94 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               <  SRR4302537.5522712/94‑1 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               >  SRR4302537.4842167/1‑94 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               <  SRR4302537.3000366/94‑1 (MQ=60)

                TGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAAC                                                                               <  SRR4302537.2685604/94‑1 (MQ=60)

                 GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAATGCGTAACGTCGCCGTAGCGACAACG                                                                              >  SRR4302537.1032631/1‑94 (MQ=60)

                 GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACG                                                                              <  SRR4302537.3163357/94‑1 (MQ=60)

                 GATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACG                                                                              >  SRR4302537.5015160/1‑94 (MQ=60)

                  ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGC                                                                             >  SRR4302537.1860991/1‑94 (MQ=60)

                  ATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGC                                                                             <  SRR4302537.2090262/94‑1 (MQ=60)

                   TCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCG                                                                            <  SRR4302537.2214474/94‑1 (MQ=60)

                    CACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGA                                                                           >  SRR4302537.4719467/1‑94 (MQ=60)

                     ACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAA                                                                          >  SRR4302537.1350918/1‑94 (MQ=60)

                       CTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATT                                                                        <  SRR4302537.5547583/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.1742555/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.415438/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.1610427/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.2562862/94‑1 (MQ=60)

                        TGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.2508130/94‑1 (MQ=60)

                        CGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTT                                                                       <  SRR4302537.1992800/94‑1 (MQ=60)

                         GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTG                                                                      >  SRR4302537.2712053/1‑94 (MQ=60)

                         GTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTG                                                                      >  SRR4302537.5841675/1‑94 (MQ=60)

                         ATACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTG                                                                      <  SRR4302537.5591435/94‑1 (MQ=60)

                          TACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGC                                                                     >  SRR4302537.3973917/1‑94 (MQ=60)

                            CCGGACAGCCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAGCGTCGCCGTAGCGACAACGCGAATTTGCGA                                                                   <  SRR4302537.5972343/94‑1 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGG                                                                 <  SRR4302537.2529212/94‑1 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGG                                                                 <  SRR4302537.4675251/94‑1 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGG                                                                 >  SRR4302537.221866/1‑94 (MQ=60)

                              GGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGG                                                                 >  SRR4302537.1299241/1‑94 (MQ=60)

                               GACAACCGAGAATTTCCTGCTCTTTGGCAATAAAATCCCCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGG                                                                <  SRR4302537.1196000/94‑1 (MQ=60)

                               GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGG                                                                <  SRR4302537.1790577/94‑1 (MQ=60)

                               GACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGG                                                                <  SRR4302537.311241/94‑1 (MQ=60)

                                ACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGA                                                               >  SRR4302537.4270185/1‑94 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCCCCCGCATTGACTGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAG                                                              <  SRR4302537.4220110/94‑1 (MQ=60)

                                 CAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCAGTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAG                                                              <  SRR4302537.897146/94‑1 (MQ=60)

                                  AACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGG                                                             >  SRR4302537.925495/1‑94 (MQ=60)

                                  AACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGG                                                             <  SRR4302537.3120795/94‑1 (MQ=60)

                                  AACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGG                                                             >  SRR4302537.2842893/1‑94 (MQ=60)

                                   ACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGA                                                            <  SRR4302537.4505423/94‑1 (MQ=60)

                                    CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAA                                                           <  SRR4302537.6433579/94‑1 (MQ=60)

                                    CCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAA                                                           >  SRR4302537.1844165/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAAT                                                          >  SRR4302537.1578731/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAAT                                                          >  SRR4302537.5203225/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAAT                                                          >  SRR4302537.3301335/1‑94 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAAT                                                          <  SRR4302537.2070203/94‑1 (MQ=60)

                                     CGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAAT                                                          <  SRR4302537.4904137/94‑1 (MQ=60)

                                      GAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATA                                                         >  SRR4302537.3889264/1‑94 (MQ=60)

                                        GAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATC                                                       <  SRR4302537.117487/94‑1 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTT                                                     <  SRR4302537.3334595/94‑1 (MQ=60)

                                          ATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTT                                                     <  SRR4302537.898308/94‑1 (MQ=60)

                                           TTTCCTGCTCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTG                                                    <  SRR4302537.2388849/94‑1 (MQ=60)

                                           TTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTG                                                    <  SRR4302537.4813631/94‑1 (MQ=60)

                                            TTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGC                                                   <  SRR4302537.4963694/94‑1 (MQ=60)

                                            TTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGC                                                   >  SRR4302537.4767300/1‑94 (MQ=60)

                                             TCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCA                                                  <  SRR4302537.4327575/94‑1 (MQ=60)

                                             TCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCA                                                  <  SRR4302537.2922613/94‑1 (MQ=60)

                                              CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAG                                                 <  SRR4302537.2478151/94‑1 (MQ=60)

                                              CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAG                                                 <  SRR4302537.3530692/94‑1 (MQ=60)

                                              CCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAG                                                 <  SRR4302537.3076625/94‑1 (MQ=60)

                                               CTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGA                                                >  SRR4302537.4597115/1‑94 (MQ=60)

                                                TGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGAC                                               >  SRR4302537.628504/1‑94 (MQ=60)

                                                  CGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGT                                             <  SRR4302537.2113743/94‑1 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            <  SRR4302537.391730/94‑1 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            <  SRR4302537.20198/94‑1 (MQ=60)

                                                   GCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTT                                            <  SRR4302537.6385534/94‑1 (MQ=60)

                                                    CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                           >  SRR4302537.1417947/1‑94 (MQ=60)

                                                    CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                           >  SRR4302537.3673810/1‑94 (MQ=60)

                                                    CTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTC                                           >  SRR4302537.3329952/1‑94 (MQ=60)

                                                     TTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          <  SRR4302537.3518583/94‑1 (MQ=60)

                                                     TATGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCA                                          <  SRR4302537.1683662/94‑1 (MQ=60)

                                                       TGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGC                                        >  SRR4302537.1885968/1‑94 (MQ=60)

                                                        GGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCA                                       >  SRR4302537.4649844/1‑94 (MQ=60)

                                                         GCAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAA                                      >  SRR4302537.136457/1‑94 (MQ=60)

                                                          CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGTGGGAGGAATATCTTGCAGACGTTCAGCAAA                                     >  SRR4302537.4772965/1‑94 (MQ=60)

                                                          CAATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAA                                     >  SRR4302537.4892271/1‑94 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    >  SRR4302537.3573000/1‑94 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    >  SRR4302537.56915/1‑94 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    >  SRR4302537.3248492/1‑94 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    <  SRR4302537.2875110/94‑1 (MQ=60)

                                                           AATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAAC                                    <  SRR4302537.613936/94‑1 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   <  SRR4302537.599374/94‑1 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   >  SRR4302537.652540/1‑94 (MQ=60)

                                                            ATAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACA                                   <  SRR4302537.883440/94‑1 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  >  SRR4302537.5693938/1‑94 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  >  SRR4302537.4834857/1‑94 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  <  SRR4302537.3675584/94‑1 (MQ=60)

                                                             TAAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAG                                  <  SRR4302537.2414469/94‑1 (MQ=60)

                                                              AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                 <  SRR4302537.4042211/94‑1 (MQ=60)

                                                              AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                 <  SRR4302537.6425709/94‑1 (MQ=60)

                                                              AAAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGG                                 <  SRR4302537.6124974/94‑1 (MQ=60)

                                                               AAATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGC                                >  SRR4302537.1282466/1‑94 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               <  SRR4302537.4399502/94‑1 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               <  SRR4302537.5060061/94‑1 (MQ=60)

                                                                AATCACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCG                               <  SRR4302537.4077727/94‑1 (MQ=60)

                                                                   CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                            >  SRR4302537.2693984/1‑94 (MQ=60)

                                                                   CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                            >  SRR4302537.3561711/1‑94 (MQ=60)

                                                                   CACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAA                            >  SRR4302537.3564833/1‑94 (MQ=60)

                                                                    ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCTACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                           >  SRR4302537.3525297/1‑94 (MQ=60)

                                                                    ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                           >  SRR4302537.3811384/1‑94 (MQ=60)

                                                                    ACCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAA                           >  SRR4302537.4798459/1‑94 (MQ=60)

                                                                     CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                          <  SRR4302537.2227575/94‑1 (MQ=60)

                                                                     CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                          <  SRR4302537.2290814/94‑1 (MQ=60)

                                                                     CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                          >  SRR4302537.2293192/1‑94 (MQ=60)

                                                                     CCCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAAC                          <  SRR4302537.2985576/94‑1 (MQ=60)

                                                                      CCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACA                         <  SRR4302537.5361815/94‑1 (MQ=60)

                                                                      CCGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACA                         >  SRR4302537.3918903/1‑94 (MQ=60)

                                                                       CGCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACAT                        <  SRR4302537.4880992/94‑1 (MQ=60)

                                                                        GCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                       <  SRR4302537.1936054/94‑1 (MQ=60)

                                                                        CCATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATT                       >  SRR4302537.6272945/1‑94 (MQ=60)

                                                                          ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     <  SRR4302537.5113122/94‑1 (MQ=60)

                                                                          ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     >  SRR4302537.3141154/1‑94 (MQ=60)

                                                                          ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     >  SRR4302537.1822158/1‑94 (MQ=60)

                                                                          ATTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGC                     >  SRR4302537.1609994/1‑94 (MQ=60)

                                                                           TTGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCC                    <  SRR4302537.3520/94‑1 (MQ=60)

                                                                            TGACGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCA                   >  SRR4302537.4421483/1‑94 (MQ=60)

                                                                              GCGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAAC                 <  SRR4302537.883092/94‑1 (MQ=60)

                                                                               CGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACC                >  SRR4302537.3261972/1‑94 (MQ=60)

                                                                               CGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACC                >  SRR4302537.2846702/1‑94 (MQ=60)

                                                                               CGGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACC                <  SRR4302537.107188/94‑1 (MQ=60)

                                                                                GGCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCG               >  SRR4302537.4839572/1‑94 (MQ=60)

                                                                                 GCAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGC              >  SRR4302537.1529715/1‑94 (MQ=60)

                                                                                  CAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCG             <  SRR4302537.5962339/94‑1 (MQ=60)

                                                                                  CAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCG             >  SRR4302537.2382142/1‑94 (MQ=60)

                                                                                  CAAGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTACAGCAAACAGGCGCAAACATTGCCAACCGCG             <  SRR4302537.4665439/94‑1 (MQ=60)

                                                                                    AGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCACACAGGCGCACACATTGCCAACCGCGCT           >  SRR4302537.2396849/1‑94 (MQ=60)

                                                                                    AGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT           >  SRR4302537.4188315/1‑94 (MQ=60)

                                                                                    AGGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCT           >  SRR4302537.2470462/1‑94 (MQ=60)

                                                                                     GGCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTC          <  SRR4302537.1065143/94‑1 (MQ=60)

                                                                                      GCGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCC         >  SRR4302537.4622974/1‑94 (MQ=60)

                                                                                       CGTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCA        >  SRR4302537.3917629/1‑94 (MQ=60)

                                                                                        GTAACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCAT       <  SRR4302537.2341776/94‑1 (MQ=60)

                                                                                          AACGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTC     >  SRR4302537.3706869/1‑94 (MQ=60)

                                                                                            CGTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCT   >  SRR4302537.5184036/1‑94 (MQ=60)

                                                                                             GTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  <  SRR4302537.4135328/94‑1 (MQ=60)

                                                                                             GTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  <  SRR4302537.5060038/94‑1 (MQ=60)

GCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCAAGGCGTAACCTCGCCGTAGCGACAACGCGAATTTGCGAGGGAGGAATATCTTGCAGACGTTCAGCAAACAGGCGCAAACATTGCCAACCGCGCTCCATTGCTT  >  NC_000913/3961495‑3961681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`