Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C99.0% 694.4 / ‑1.4 204coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (106/96);  total (107/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 218 total aligned reads displayed.

GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑T‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567
                                                                                              |                                                                                        
       cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑T‑ccccaac                                                                                   <  2:4292998/93‑5 (MQ=255)
           gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCa                                                                               <  1:1112322/93‑1 (MQ=255)
           gctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCa                                                                               >  2:1377707/1‑93 (MQ=255)
            ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCAc                                                                              <  1:761617/93‑1 (MQ=255)
            ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCAc                                                                              <  1:1611920/93‑1 (MQ=255)
            ctgGATCCCCAGCCAAATCAAGCAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCAc                                                                              <  2:311466/93‑1 (MQ=255)
             tgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACg                                                                             >  1:1308798/1‑93 (MQ=255)
              ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGc                                                                            >  2:3639996/1‑93 (MQ=255)
              ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGc                                                                            <  2:3330632/93‑1 (MQ=255)
               gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGcc                                                                           >  2:4238409/1‑93 (MQ=255)
               gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGcc                                                                           >  2:600249/1‑93 (MQ=255)
                  tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAAc                                                                        <  2:5767836/93‑1 (MQ=255)
                    ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCa                                                                      <  1:4627703/93‑1 (MQ=255)
                    ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCa                                                                      >  1:5663877/1‑93 (MQ=255)
                      aGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGc                                                                    <  1:4767963/93‑1 (MQ=255)
                      aGCCAAATAAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGc                                                                    <  1:5424303/93‑1 (MQ=255)
                       gCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGca                                                                   >  2:2512136/1‑93 (MQ=255)
                        gaaaaTGAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGcac                                                                  <  2:2706287/91‑1 (MQ=255)
                        ccAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCGACCAGcac                                                                  >  2:159152/1‑93 (MQ=255)
                          aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACaa                                                                <  1:3347803/93‑1 (MQ=255)
                          aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACaa                                                                <  1:1171976/93‑1 (MQ=255)
                          aaaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑T‑CCCGAACCCCACGCCAACCAGCACaa                                                                >  2:5426905/1‑93 (MQ=255)
                           aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAat                                                               >  1:1381845/1‑93 (MQ=255)
                            aTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGACAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAata                                                              >  2:1532268/1‑93 (MQ=255)
                             tCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAatat                                                             <  1:3077274/93‑1 (MQ=255)
                               aaGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGa                                                           <  2:113885/93‑1 (MQ=255)
                               aaGTAAGCCGAGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGa                                                           >  2:4870503/1‑93 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGGGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAAt                                                         >  2:6017871/1‑93 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAAt                                                         >  1:723786/1‑93 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAAt                                                         <  1:838919/93‑1 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAAt                                                         <  1:248008/93‑1 (MQ=255)
                                  tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATc                                                        <  2:4978020/93‑1 (MQ=255)
                                  tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATc                                                        <  2:442945/93‑1 (MQ=255)
                                  tAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATc                                                        >  1:4170986/1‑93 (MQ=255)
                                   aaGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCg                                                       <  2:2176472/93‑1 (MQ=255)
                                      ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCa                                                    <  1:4315349/93‑1 (MQ=255)
                                       cgcgCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                   <  1:6372285/93‑1 (MQ=255)
                                        gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                  <  2:1242425/93‑1 (MQ=255)
                                         cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 >  2:2365673/1‑93 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  1:444480/1‑93 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  1:999171/1‑93 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  1:3661614/1‑93 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  1:1676990/1‑93 (MQ=255)
                                           ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               >  1:6142684/1‑93 (MQ=255)
                                           ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               >  2:2416796/1‑93 (MQ=255)
                                           ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               <  1:2169840/93‑1 (MQ=255)
                                            cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              <  2:3737352/93‑1 (MQ=255)
                                            cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              >  1:2881307/1‑93 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  1:292616/93‑1 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  1:5988876/93‑1 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  1:4843328/93‑1 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  2:71281/93‑1 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           <  1:3703443/93‑1 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  2:5357754/1‑93 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           <  1:3792724/93‑1 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  1:1277564/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  2:1231312/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  1:5705854/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAAAGCAATCCCTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCCGCCCAATATGAATCGCCAGTCCGGTCTg                                          >  1:2075760/1‑93 (MQ=255)
                                                 cacttcaacacttcaaACGCACTCACTGAGAGGGAAAATACCG‑TCCCC‑AACCCCACGCCAACCAGCACACTATGAATCGCTCGTCCGGTCTgc                                         >  2:3939455/1‑92 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         <  1:4963519/93‑1 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACGGAGCGGGAAAATAAGG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGCATCGCCAGTCCGGTCTGa                                         >  2:3277793/1‑93 (MQ=255)
                                                 cactccaacactacaaACGAAATCACGGAGGGGAAAAATAGCGCC‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         <  2:5674349/93‑1 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  1:4584940/1‑93 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        <  1:4028673/93‑1 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  2:5121236/1‑93 (MQ=255)
                                                   cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAg                                       >  2:353684/1‑93 (MQ=255)
                                                    ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      >  2:4949288/1‑93 (MQ=255)
                                                      catcacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                    <  1:5708411/90‑1 (MQ=255)
                                                      caacacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                    <  2:5276741/93‑1 (MQ=255)
                                                        acacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  2:2976945/1‑93 (MQ=255)
                                                        acacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAg                                  >  2:2808194/1‑93 (MQ=255)
                                                         cacttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGc                                 >  2:206366/1‑93 (MQ=255)
                                                          acttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                <  2:5233631/93‑1 (MQ=255)
                                                           cttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                               <  1:1054473/93‑1 (MQ=255)
                                                            ttcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAt                              >  2:4657181/1‑93 (MQ=255)
                                                             tcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATa                             <  1:5247459/93‑1 (MQ=255)
                                                             tcaaACGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATa                             <  1:3928797/93‑1 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           >  1:4713955/1‑93 (MQ=255)
                                                                aaCGCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                          >  2:5003365/1‑93 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       >  2:3842446/1‑93 (MQ=255)
                                                                      aTCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  1:3967691/1‑93 (MQ=255)
                                                                       tCACTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                   >  1:4499187/1‑93 (MQ=255)
                                                                         aCTGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcg                 >  1:4198711/1‑93 (MQ=255)
                                                                           tGAGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               >  1:3002387/1‑93 (MQ=255)
                                                                             aGCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgc             <  2:6424420/93‑1 (MQ=255)
                                                                              gCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATCTGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg            >  1:3467636/1‑93 (MQ=255)
                                                                              gCGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg            >  2:5306961/1‑93 (MQ=255)
                                                                               cGGGAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGa           >  2:6408631/1‑93 (MQ=255)
                                                                                 ggAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  2:766851/1‑93 (MQ=255)
                                                                                 ggAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  1:4411698/1‑93 (MQ=255)
                                                                                 ggAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         <  1:4896117/93‑1 (MQ=255)
                                                                                  gAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATaa        >  2:5880821/1‑93 (MQ=255)
                                                                                  gAAAATAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATaa        <  1:1536518/93‑1 (MQ=255)
                                                                                      aTAGCG‑TCCCC‑AACCCCCCGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  1:3014986/93‑1 (MQ=255)
                                                                                      aTAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCgg    >  2:5379292/1‑92 (MQ=255)
                                                                                      aTAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCgg    >  1:3757211/1‑92 (MQ=255)
                                                                                      aTAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    <  1:579707/93‑1 (MQ=255)
                                                                                      aTAGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGt    >  2:1063938/1‑93 (MQ=255)
                                                                                        aGCG‑TCCCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTgg  >  2:638326/1‑93 (MQ=255)
                                                                                              |                                                                                        
GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCG‑T‑CCC‑AACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA  >  NC_000913/4006391‑4006575
                                                                                             |                                                                                            
GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACCGAGCGGGAAAATAGCGT‑CCCC                                                                                             <  SRR4302537.6089698/94‑1 (MQ=60)
   CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAC                                                                                           >  SRR4302537.1260973/1‑94 (MQ=60)
   CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAC                                                                                           >  SRR4302537.785390/1‑94 (MQ=60)
   CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAC                                                                                           >  SRR4302537.4384525/1‑94 (MQ=60)
    CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACC                                                                                          >  SRR4302537.3246834/1‑94 (MQ=60)
    CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACC                                                                                          >  SRR4302537.5025870/1‑94 (MQ=60)
    CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACC                                                                                          >  SRR4302537.6132109/1‑94 (MQ=60)
    CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACC                                                                                          <  SRR4302537.2169458/94‑1 (MQ=60)
    CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACC                                                                                          >  SRR4302537.4924539/1‑94 (MQ=60)
     ACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCccaccc                                                                                        >  SRR4302537.3855192/1‑88 (MQ=60)
        GCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCAC                                                                                      <  SRR4302537.744075/94‑1 (MQ=60)
        GCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCAC                                                                                      <  SRR4302537.1120004/94‑1 (MQ=60)
         CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACG                                                                                     <  SRR4302537.2790880/94‑1 (MQ=60)
         CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACG                                                                                     >  SRR4302537.1317908/1‑94 (MQ=60)
         CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACG                                                                                     <  SRR4302537.766822/94‑1 (MQ=60)
         CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACG                                                                                     <  SRR4302537.1623185/94‑1 (MQ=60)
         CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACG                                                                                     >  SRR4302537.303226/1‑94 (MQ=60)
                CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCA                                                                              <  SRR4302537.277794/94‑1 (MQ=60)
                CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCA                                                                              >  SRR4302537.5702632/1‑94 (MQ=60)
                 CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAG                                                                             <  SRR4302537.4008709/94‑1 (MQ=60)
                 CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAG                                                                             <  SRR4302537.4695026/94‑1 (MQ=60)
                 CCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAG                                                                             <  SRR4302537.4659722/94‑1 (MQ=60)
                  CAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGC                                                                            >  SRR4302537.4969530/1‑94 (MQ=60)
                   AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCA                                                                           <  SRR4302537.4800886/94‑1 (MQ=60)
                   AGCCAAATAAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCA                                                                           <  SRR4302537.5461274/94‑1 (MQ=60)
                     CCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACA                                                                         <  SRR4302537.4541552/94‑1 (MQ=60)
                      CAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAA                                                                        <  SRR4302537.3903720/94‑1 (MQ=60)
                       AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAAT                                                                       >  SRR4302537.1718443/1‑94 (MQ=60)
                       AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAAT                                                                       <  SRR4302537.3371503/94‑1 (MQ=60)
                       AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAAT                                                                       >  SRR4302537.1391486/1‑94 (MQ=60)
                       AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAAT                                                                       <  SRR4302537.1180133/94‑1 (MQ=60)
                        AATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATA                                                                      >  SRR4302537.1658525/1‑94 (MQ=60)
                         ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATAT                                                                     <  SRR4302537.5037108/94‑1 (MQ=60)
                          TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATG                                                                    <  SRR4302537.3805541/94‑1 (MQ=60)
                          TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATG                                                                    >  SRR4302537.1385063/1‑94 (MQ=60)
                          TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATG                                                                    <  SRR4302537.3098710/94‑1 (MQ=60)
                             AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAAT                                                                 >  SRR4302537.728728/1‑94 (MQ=60)
                              GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATC                                                                <  SRR4302537.844666/94‑1 (MQ=60)
                              GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATC                                                                <  SRR4302537.249769/94‑1 (MQ=60)
                              GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATC                                                                >  SRR4302537.4200117/1‑94 (MQ=60)
                                 AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCC                                                             >  SRR4302537.5707386/1‑94 (MQ=60)
                                   CCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAG                                                           <  SRR4302537.4345346/94‑1 (MQ=60)
                                    CGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                          >  SRR4302537.3312568/1‑94 (MQ=60)
                                    CGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                          <  SRR4302537.6415744/94‑1 (MQ=60)
                                      CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                        >  SRR4302537.1006067/1‑94 (MQ=60)
                                      CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                        >  SRR4302537.1162388/1‑94 (MQ=60)
                                      CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                        >  SRR4302537.3687386/1‑94 (MQ=60)
                                      CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                        >  SRR4302537.447567/1‑94 (MQ=60)
                                      CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                        >  SRR4302537.1688729/1‑94 (MQ=60)
                                       GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                       <  SRR4302537.4869477/94‑1 (MQ=60)
                                       GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                       <  SRR4302537.5315869/94‑1 (MQ=60)
                                       GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                       >  SRR4302537.6184921/1‑94 (MQ=60)
                                        CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                      >  SRR4302537.2901530/1‑94 (MQ=60)
                                        CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                      <  SRR4302537.2185131/94‑1 (MQ=60)
                                         CTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGT                                                     >  SRR4302537.3464583/1‑94 (MQ=60)
                                          TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                    >  SRR4302537.1302364/1‑94 (MQ=60)
                                           TCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                   <  SRR4302537.1543693/94‑1 (MQ=60)
                                           GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                   <  SRR4302537.6030005/94‑1 (MQ=60)
                                           GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                   <  SRR4302537.294657/94‑1 (MQ=60)
                                           GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                   >  SRR4302537.1286440/1‑94 (MQ=60)
                                           GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                   <  SRR4302537.4876757/94‑1 (MQ=60)
                                            CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                  <  SRR4302537.3819344/94‑1 (MQ=60)
                                            CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                  <  SRR4302537.3729488/94‑1 (MQ=60)
                                            CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                  >  SRR4302537.5744903/1‑94 (MQ=60)
                                            CCCACTTCAACACTTCAAAAGCAATCCCTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCCGCCCAATATGAATCGCCAGTCCGGTCTG                                                  >  SRR4302537.2090376/1‑94 (MQ=60)
                                              CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                <  SRR4302537.4997734/94‑1 (MQ=60)
                                              CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                >  SRR4302537.4622633/1‑94 (MQ=60)
                                              CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                >  SRR4302537.4616654/1‑94 (MQ=60)
                                               ACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAG                                               <  SRR4302537.4056874/94‑1 (MQ=60)
                                                CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                              >  SRR4302537.375387/1‑94 (MQ=60)
                                                CTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGC                                              <  SRR4302537.942979/94‑1 (MQ=60)
                                                  TCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCC                                            >  SRR4302537.5746813/1‑94 (MQ=60)
                                                   CATCACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                           <  SRR4302537.5747473/94‑1 (MQ=60)
                                                   CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                           >  SRR4302537.80236/1‑94 (MQ=60)
                                                   CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                           >  SRR4302537.645973/1‑94 (MQ=60)
                                                   CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                           >  SRR4302537.3585519/1‑94 (MQ=60)
                                                   CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                           >  SRR4302537.5751971/1‑94 (MQ=60)
                                                    AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAG                                          >  SRR4302537.3923431/1‑94 (MQ=60)
                                                    AACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTAAAGCCCAG                                          >  SRR4302537.2931319/1‑94 (MQ=60)
                                                       ACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCACCAA                                       >  SRR4302537.5630478/1‑94 (MQ=60)
                                                        CTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                      <  SRR4302537.1061749/94‑1 (MQ=60)
                                                         TTCAAACGCAATCACTGAGCGGGAAAGTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                     <  SRR4302537.598273/94‑1 (MQ=60)
                                                         TTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                     >  SRR4302537.6355381/1‑94 (MQ=60)
                                                          TCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAG                                    <  SRR4302537.3956249/94‑1 (MQ=60)
                                                          TCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAG                                    <  SRR4302537.5283567/94‑1 (MQ=60)
                                                           CAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                   >  SRR4302537.4746559/1‑94 (MQ=60)
                                                           CAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                   <  SRR4302537.3688098/94‑1 (MQ=60)
                                                             AACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                 <  SRR4302537.5215106/94‑1 (MQ=60)
                                                             AACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                 <  SRR4302537.4222028/94‑1 (MQ=60)
                                                              ACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGC                                >  SRR4302537.1454274/1‑94 (MQ=60)
                                                              ACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGC                                >  SRR4302537.4318001/1‑94 (MQ=60)
                                                               CGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCC                               >  SRR4302537.4775456/1‑94 (MQ=60)
                                                                GCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCA                              >  SRR4302537.4447416/1‑94 (MQ=60)
                                                                  AATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                            >  SRR4302537.3995453/1‑94 (MQ=60)
                                                                  AATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                            >  SRR4302537.3995454/1‑94 (MQ=60)
                                                                   ATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                           >  SRR4302537.4530304/1‑94 (MQ=60)
                                                                     CACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCG                         >  SRR4302537.4228028/1‑94 (MQ=60)
                                                                      ACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGC                        >  SRR4302537.3062457/1‑94 (MQ=60)
                                                                       CTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                       >  SRR4302537.2474972/1‑94 (MQ=60)
                                                                       CTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                       >  SRR4302537.3023305/1‑94 (MQ=60)
                                                                          AGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATCTGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                    >  SRR4302537.3492165/1‑94 (MQ=60)
                                                                          AGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                    >  SRR4302537.490296/1‑94 (MQ=60)
                                                                          AGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                    <  SRR4302537.4275239/94‑1 (MQ=60)
                                                                             GGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                 >  SRR4302537.4442144/1‑94 (MQ=60)
                                                                              GGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAA                <  SRR4302537.4929920/94‑1 (MQ=60)
                                                                               GAAAATAGCGTCCCCAACCCCGCGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC               <  SRR4302537.4491734/94‑1 (MQ=60)
                                                                               GAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAC               <  SRR4302537.1547249/94‑1 (MQ=60)
                                                                                AAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACC              <  SRR4302537.4116988/94‑1 (MQ=60)
                                                                                AAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACC              <  SRR4302537.3329695/94‑1 (MQ=60)
                                                                                 AAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCG             <  SRR4302537.158357/94‑1 (MQ=60)
                                                                                 AAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCG             <  SRR4302537.4695143/94‑1 (MQ=60)
                                                                                  AATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT            <  SRR4302537.1095479/94‑1 (MQ=60)
                                                                                  AATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGG            >  SRR4302537.3783586/1‑94 (MQ=60)
                                                                                   ATAGCGTCCCCAACCCCCCGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG           <  SRR4302537.3036006/94‑1 (MQ=60)
                                                                                   ATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG           >  SRR4302537.6000488/1‑94 (MQ=60)
                                                                                   ATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG           <  SRR4302537.583673/94‑1 (MQ=60)
                                                                                   ATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTG           <  SRR4302537.2976732/94‑1 (MQ=60)
                                                                                     AGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAATCCCAGCAATAGACGCCACCGCGCCACGATAACCGTGGT         >  SRR4302537.795038/1‑94 (MQ=60)
                                                                                     AGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT         >  SRR4302537.2000615/1‑94 (MQ=60)
                                                                                     AGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT         >  SRR4302537.3786392/1‑94 (MQ=60)
                                                                                       CGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG       <  SRR4302537.761015/94‑1 (MQ=60)
                                                                                        gtcCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA      >  SRR4302537.197337/4‑94 (MQ=60)
                                                                                         tcCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAATCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAG     >  SRR4302537.5284034/3‑94 (MQ=60)
                                                                                         C‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC    <  SRR4302537.129315/94‑1 (MQ=60)
                                                                                         C‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGC    >  SRR4302537.5372117/1‑94 (MQ=60)
                                                                                           CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG   >  SRR4302537.4772567/1‑94 (MQ=60)
                                                                                           CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCACAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG   <  SRR4302537.5138110/94‑1 (MQ=60)
                                                                                            CCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA  >  SRR4302537.4614723/1‑94 (MQ=60)
                                                                                             |                                                                                            
GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA  >  NC_000913/4006391‑4006575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑