Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,058,433 A→G G136G (GGA→GGG)  yihL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,058,4330AG96.2% 747.4 / 12.7 234G136G (GGA→GGG) yihLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (3/6);  new base G (108/117);  total (111/123)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 236 total aligned reads displayed.

CAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGT  >  NC_000913/4058343‑4058522
                                                                                          |                                                                                         
cAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCa                                                                                         <  2:5440026/93‑1 (MQ=255)
 aGCGATAGTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCAt                                                                                        <  1:4972820/93‑1 (MQ=255)
  gCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATa                                                                                       <  2:465438/93‑1 (MQ=255)
  gCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATa                                                                                       >  2:4262755/1‑93 (MQ=255)
    gATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATaaa                                                                                     <  1:6302430/93‑1 (MQ=255)
     atatTCCCGAGGCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAg                                                                                    <  2:5262512/93‑1 (MQ=255)
     atatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGTGGCGCTGGAAGGGCATAAAg                                                                                    <  2:2492404/93‑1 (MQ=255)
     atatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAg                                                                                    <  2:491491/93‑1 (MQ=255)
     atatTCCCGAGACGCTCCAGCCACTAATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCCGCTGGGGGGCGCTGGAAGGACATAAAg                                                                                    >  2:4567822/1‑93 (MQ=255)
      tgttCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGt                                                                                   <  1:4401823/91‑1 (MQ=255)
      tatTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGt                                                                                   >  1:5182486/1‑93 (MQ=255)
          cccGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTc                                                                               <  1:3271490/93‑1 (MQ=255)
          cccGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTc                                                                               <  2:5152077/93‑1 (MQ=255)
            cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTa                                                                             >  2:4115869/1‑93 (MQ=255)
            cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTa                                                                             >  2:1227926/1‑93 (MQ=255)
            cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTa                                                                             <  2:1580927/93‑1 (MQ=255)
            cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTa                                                                             <  2:3672141/93‑1 (MQ=255)
            cGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTa                                                                             >  1:2368953/1‑93 (MQ=255)
             gagaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGGTTTCTAt                                                                            >  2:5463809/1‑93 (MQ=255)
               gaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCa                                                                          >  2:795373/1‑93 (MQ=255)
               gaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCa                                                                          <  2:5575912/93‑1 (MQ=255)
               gaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGATTTCTATCa                                                                          >  2:282259/1‑93 (MQ=255)
                 cGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACg                                                                        >  1:4899206/1‑93 (MQ=255)
                  gCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGa                                                                       >  1:6406742/1‑93 (MQ=255)
                   cTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGaa                                                                      >  2:5913582/1‑93 (MQ=255)
                   cTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGaa                                                                      >  1:5811584/1‑93 (MQ=255)
                    tCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGaaa                                                                     >  2:5287459/1‑93 (MQ=255)
                      gcgcCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACa                                                                   <  2:2387076/93‑1 (MQ=255)
                      gcgcCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACa                                                                   <  2:3813220/93‑1 (MQ=255)
                       cgcCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACat                                                                  <  1:4683938/93‑1 (MQ=255)
                         ccACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACatat                                                                >  2:3078661/1‑93 (MQ=255)
                         ccACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACatat                                                                <  1:615903/93‑1 (MQ=255)
                         ccACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACatat                                                                >  2:4554890/1‑93 (MQ=255)
                          cACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACatata                                                               >  1:4447295/1‑93 (MQ=255)
                          cACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACatata                                                               <  2:1990039/93‑1 (MQ=255)
                          cACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACatata                                                               >  2:6165939/1‑93 (MQ=255)
                             tGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTa                                                            >  1:2609158/1‑93 (MQ=255)
                             tGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTa                                                            <  1:1816381/93‑1 (MQ=255)
                             tGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTa                                                            <  2:2172083/93‑1 (MQ=255)
                              gATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTaa                                                           >  1:539558/1‑93 (MQ=255)
                              gATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTaa                                                           >  2:1262670/1‑93 (MQ=255)
                                     gTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGaa                                                    >  1:4772243/1‑93 (MQ=255)
                                      tGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAg                                                   <  1:3952947/93‑1 (MQ=255)
                                       gACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGt                                                  <  2:4477961/93‑1 (MQ=255)
                                        aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGtt                                                 >  2:3467202/1‑93 (MQ=255)
                                        aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGtt                                                 <  2:2584826/93‑1 (MQ=255)
                                        aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGtt                                                 <  2:5594374/93‑1 (MQ=255)
                                          gCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGGTTTCTATCACGATACATCTATTAATCCTGAAGTTGc                                               >  1:5348562/1‑93 (MQ=255)
                                          gCCATCAACTCAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGc                                               >  2:1451932/1‑93 (MQ=255)
                                           ccATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCt                                              <  1:1930199/93‑1 (MQ=255)
                                            cATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTc                                             >  2:1725674/1‑93 (MQ=255)
                                             aTCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTcc                                            >  1:689450/1‑93 (MQ=255)
                                             aTCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTcc                                            <  2:2982654/93‑1 (MQ=255)
                                             aTCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTcc                                            >  2:2671670/1‑93 (MQ=255)
                                              tCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCg                                           >  1:5647008/1‑93 (MQ=255)
                                                 aCTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGc                                        >  2:1075902/1‑93 (MQ=255)
                                                 aCTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGc                                        >  1:1013307/1‑93 (MQ=255)
                                                 aCTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGATGCTCCGGGc                                        >  1:5618578/1‑93 (MQ=255)
                                                   tGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCtt                                      >  1:3995514/1‑93 (MQ=255)
                                                    gAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCttt                                     <  2:5845785/93‑1 (MQ=255)
                                                     aaCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTa                                    <  2:665734/93‑1 (MQ=255)
                                                     aaCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTa                                    <  2:4142824/93‑1 (MQ=255)
                                                     aaCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTa                                    >  2:3887540/1‑93 (MQ=255)
                                                     aaCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTa                                    <  2:6079145/93‑1 (MQ=255)
                                                      aCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAt                                   >  1:538332/1‑93 (MQ=255)
                                                      aCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGATTTAt                                   >  2:1884186/1‑93 (MQ=255)
                                                       ctctATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAtt                                  >  1:2916114/1‑93 (MQ=255)
                                                       ctctATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAtt                                  >  1:2154298/1‑93 (MQ=255)
                                                        tctATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTg                                 <  1:3474127/93‑1 (MQ=255)
                                                         ctATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGa                                <  1:1644523/93‑1 (MQ=255)
                                                          tATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaa                               <  2:629385/93‑1 (MQ=255)
                                                           aTCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaac                              <  2:3331399/93‑1 (MQ=255)
                                                             cGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaacaa                            <  1:4368531/93‑1 (MQ=255)
                                                               cATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCTGGCTTTATTGAACAACt                          <  2:3259747/93‑1 (MQ=255)
                                                               cATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACt                          <  2:5653432/93‑1 (MQ=255)
                                                               cATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACt                          <  1:1087956/93‑1 (MQ=255)
                                                               cATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACt                          >  1:2342588/1‑93 (MQ=255)
                                                                aTCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                         >  1:3787964/1‑93 (MQ=255)
                                                                aTCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACtt                         <  1:4214248/93‑1 (MQ=255)
                                                                 tCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTg                        >  2:5979131/1‑93 (MQ=255)
                                                                  cACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGa                       <  1:3071984/93‑1 (MQ=255)
                                                                  cACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGa                       <  2:3622487/93‑1 (MQ=255)
                                                                    ccGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaaa                     >  1:453528/1‑93 (MQ=255)
                                                                     cGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGaaaa                    <  1:2664874/93‑1 (MQ=255)
                                                                      ggCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAAc                   <  1:602888/93‑1 (MQ=255)
                                                                      ggCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAAc                   <  1:3368652/93‑1 (MQ=255)
                                                                       gCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAAcc                  >  1:3509430/1‑93 (MQ=255)
                                                                         tGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCAc                <  2:3910672/93‑1 (MQ=255)
                                                                         tGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCAc                <  1:4320038/93‑1 (MQ=255)
                                                                         tGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCAc                <  1:790887/93‑1 (MQ=255)
                                                                          ggggggCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACt               <  1:1719136/93‑1 (MQ=255)
                                                                           gggggCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTc              >  2:2143519/1‑93 (MQ=255)
                                                                              ggCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCAtt           >  2:4714993/1‑93 (MQ=255)
                                                                                 gcTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTc        <  1:6278907/93‑1 (MQ=255)
                                                                                  cTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCt       <  1:1375635/93‑1 (MQ=255)
                                                                                  cTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCt       <  1:5913582/93‑1 (MQ=255)
                                                                                   tGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg      >  2:3056481/1‑93 (MQ=255)
                                                                                   tGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg      >  1:1191114/1‑93 (MQ=255)
                                                                                   tGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTg      >  1:1561903/1‑93 (MQ=255)
                                                                                     gAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCa    <  2:1121394/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGT  >  NC_000913/4058343‑4058522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGG  >  NC_000913/4058340‑4058526
                                                                                             |                                                                                             
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGG                                                                                               <  SRR4302537.1684683/94‑1 (MQ=60)
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGG                                                                                               <  SRR4302537.3746137/94‑1 (MQ=60)
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGG                                                                                               >  SRR4302537.3069018/1‑94 (MQ=60)
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGG                                                                                               <  SRR4302537.1298299/94‑1 (MQ=60)
 ACCGGCGATATTCCGGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGC                                                                                              <  SRR4302537.1321004/94‑1 (MQ=60)
  CCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCA                                                                                             <  SRR4302537.2651057/94‑1 (MQ=60)
    AGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATA                                                                                           >  SRR4302537.1228241/1‑94 (MQ=60)
    AGCGATAGTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATA                                                                                           <  SRR4302537.5007094/94‑1 (MQ=60)
     GCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAA                                                                                          <  SRR4302537.2901850/94‑1 (MQ=60)
     GCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAA                                                                                          <  SRR4302537.4527607/94‑1 (MQ=60)
       GATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAG                                                                                        <  SRR4302537.6345590/94‑1 (MQ=60)
       GATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAG                                                                                        <  SRR4302537.2467169/94‑1 (MQ=60)
        ATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGT                                                                                       >  SRR4302537.5218147/1‑94 (MQ=60)
         TGTTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTT                                                                                      <  SRR4302537.4432197/94‑1 (MQ=60)
             CCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCT                                                                                  <  SRR4302537.108514/94‑1 (MQ=60)
             CCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCT                                                                                  >  SRR4302537.171223/1‑94 (MQ=60)
             CCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCT                                                                                  <  SRR4302537.3294578/94‑1 (MQ=60)
             CCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCT                                                                                  <  SRR4302537.5987017/94‑1 (MQ=60)
              CCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGGCTGGAAGGGCATAAAGTTTTCTA                                                                                 >  SRR4302537.5422090/1‑94 (MQ=60)
              CCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTA                                                                                 >  SRR4302537.2385649/1‑94 (MQ=60)
                  GGCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCAC                                                                             <  SRR4302537.439634/94‑1 (MQ=60)
                  GACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCAC                                                                             >  SRR4302537.2958930/1‑94 (MQ=60)
                   ACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACG                                                                            >  SRR4302537.4933024/1‑94 (MQ=60)
                    CGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGA                                                                           >  SRR4302537.6450345/1‑94 (MQ=60)
                    CGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGA                                                                           >  SRR4302537.5745909/1‑94 (MQ=60)
                     GCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAA                                                                          >  SRR4302537.5851426/1‑94 (MQ=60)
                      CTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAA                                                                         <  SRR4302537.2229030/94‑1 (MQ=60)
                          CGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATA                                                                     <  SRR4302537.4716336/94‑1 (MQ=60)
                           GCNANTNANTGNANTGACGNCATCAACTGANCTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATAT                                                                    <  SRR4302537.168392/94‑1 (MQ=60)
                           GCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATAT                                                                    <  SRR4302537.3347595/94‑1 (MQ=60)
                           GCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATAT                                                                    >  SRR4302537.446271/1‑94 (MQ=60)
                           GCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATAT                                                                    >  SRR4302537.5778696/1‑94 (MQ=60)
                            CCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATA                                                                   >  SRR4302537.1156672/1‑94 (MQ=60)
                            CCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATA                                                                   >  SRR4302537.4478007/1‑94 (MQ=60)
                            CCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATA                                                                   >  SRR4302537.3354243/1‑94 (MQ=60)
                            CCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATA                                                                   >  SRR4302537.6331472/1‑94 (MQ=60)
                            CCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATA                                                                   <  SRR4302537.620099/94‑1 (MQ=60)
                             CACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATAT                                                                  >  SRR4302537.1327994/1‑94 (MQ=60)
                             CACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATAT                                                                  >  SRR4302537.711917/1‑94 (MQ=60)
                             CACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATAT                                                                  >  SRR4302537.6182190/1‑94 (MQ=60)
                               CTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTA                                                                >  SRR4302537.2627407/1‑94 (MQ=60)
                               CTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTA                                                                <  SRR4302537.4945276/94‑1 (MQ=60)
                                TGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAA                                                               <  SRR4302537.1829041/94‑1 (MQ=60)
                                TGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAA                                                               >  SRR4302537.543270/1‑94 (MQ=60)
                                 GATTGCAGTGACGCGATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAAT                                                              <  SRR4302537.5139156/94‑1 (MQ=60)
                                  ATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGGCTGGAAGGACATAAAGATTTCTATCACGAAACATATATTAATC                                                             >  SRR4302537.3560604/1‑94 (MQ=60)
                                  ATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATC                                                             >  SRR4302537.1735904/1‑94 (MQ=60)
                                     GCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTG                                                          <  SRR4302537.657291/94‑1 (MQ=60)
                                      CAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGA                                                         >  SRR4302537.943173/1‑94 (MQ=60)
                                       AGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAA                                                        >  SRR4302537.4193436/1‑94 (MQ=60)
                                       AGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAA                                                        >  SRR4302537.952374/1‑94 (MQ=60)
                                       AGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAA                                                        >  SRR4302537.4805194/1‑94 (MQ=60)
                                        GTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAG                                                       <  SRR4302537.4392390/94‑1 (MQ=60)
                                         TGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGT                                                      <  SRR4302537.3980599/94‑1 (MQ=60)
                                          GACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTT                                                     <  SRR4302537.540518/94‑1 (MQ=60)
                                           ACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTG                                                    <  SRR4302537.1905417/94‑1 (MQ=60)
                                            CGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGGTTTCTATCACGATACATCTATTAATCCTGAAGTTGC                                                   >  SRR4302537.5385208/1‑94 (MQ=60)
                                              CCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTC                                                 >  SRR4302537.1476310/1‑94 (MQ=60)
                                              CCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTC                                                 <  SRR4302537.1943595/94‑1 (MQ=60)
                                               CATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCC                                                >  SRR4302537.694175/1‑94 (MQ=60)
                                               CATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCC                                                <  SRR4302537.6103728/94‑1 (MQ=60)
                                               CATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCC                                                <  SRR4302537.6410688/94‑1 (MQ=60)
                                                ATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCG                                               >  SRR4302537.5685655/1‑94 (MQ=60)
                                                ATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCG                                               <  SRR4302537.4629697/94‑1 (MQ=60)
                                                  CAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGG                                             >  SRR4302537.5866737/1‑94 (MQ=60)
                                                   AACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGC                                            >  SRR4302537.1020291/1‑94 (MQ=60)
                                                   AACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGATGCTCCGGGC                                            >  SRR4302537.5656999/1‑94 (MQ=60)
                                                    ACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCT                                           >  SRR4302537.1647222/1‑94 (MQ=60)
                                                    ACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCT                                           <  SRR4302537.2865720/94‑1 (MQ=60)
                                                    ACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATAACGAAACATATATTAATCCTGAAGTTGCTCCGGGCT                                           >  SRR4302537.1700340/1‑94 (MQ=60)
                                                     CTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTT                                          >  SRR4302537.4023479/1‑94 (MQ=60)
                                                        AACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAT                                       >  SRR4302537.542025/1‑94 (MQ=60)
                                                        AACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAT                                       >  SRR4302537.4427483/1‑94 (MQ=60)
                                                        AACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTAT                                       <  SRR4302537.6357782/94‑1 (MQ=60)
                                                         ACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATT                                      >  SRR4302537.2169482/1‑94 (MQ=60)
                                                         ACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATT                                      >  SRR4302537.2936599/1‑94 (MQ=60)
                                                         ACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATT                                      >  SRR4302537.3515024/1‑94 (MQ=60)
                                                         ACTCTATCGCATCACCGGCGGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATT                                      <  SRR4302537.2672492/94‑1 (MQ=60)
                                                          CTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTG                                     <  SRR4302537.4043619/94‑1 (MQ=60)
                                                          CTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTG                                     <  SRR4302537.6231929/94‑1 (MQ=60)
                                                           TCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGA                                    <  SRR4302537.3498709/94‑1 (MQ=60)
                                                            CTATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAA                                   <  SRR4302537.5487953/94‑1 (MQ=60)
                                                            CTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAA                                   <  SRR4302537.1656046/94‑1 (MQ=60)
                                                             TATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAAC                                  >  SRR4302537.4294152/1‑94 (MQ=60)
                                                             TATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAAC                                  >  SRR4302537.3987740/1‑94 (MQ=60)
                                                             TATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACAAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAAC                                  <  SRR4302537.2011862/94‑1 (MQ=60)
                                                              ATCGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACA                                 <  SRR4302537.5503905/94‑1 (MQ=60)
                                                                CGCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAAC                               <  SRR4302537.4398723/94‑1 (MQ=60)
                                                                 GCATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACT                              >  SRR4302537.2359094/1‑94 (MQ=60)
                                                                  CATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTT                             >  SRR4302537.3814558/1‑94 (MQ=60)
                                                                  CATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTT                             <  SRR4302537.1095470/94‑1 (MQ=60)
                                                                  CATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTT                             <  SRR4302537.4809483/94‑1 (MQ=60)
                                                                   ATCACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTG                            <  SRR4302537.4243635/94‑1 (MQ=60)
                                                                     CACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAA                          <  SRR4302537.3093384/94‑1 (MQ=60)
                                                                      ACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAA                         >  SRR4302537.3132422/1‑94 (MQ=60)
                                                                      ACCGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAA                         >  SRR4302537.456663/1‑94 (MQ=60)
                                                                        CGGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAAC                       <  SRR4302537.2683488/94‑1 (MQ=60)
                                                                         GGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACC                      >  SRR4302537.3534259/1‑94 (MQ=60)
                                                                         GGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACC                      <  SRR4302537.607013/94‑1 (MQ=60)
                                                                         GGCTGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACC                      <  SRR4302537.3392513/94‑1 (MQ=60)
                                                                            TGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACT                   <  SRR4302537.4350070/94‑1 (MQ=60)
                                                                            TGGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACT                   <  SRR4302537.796317/94‑1 (MQ=60)
                                                                            TGGGGGGAGCTCGAAGTGCATAAAGTTTTCTATCACGAATGATATATGAATCCTGAAGTGGCGCCGGGCTTTATTGAACAACTTGAAAACCACT                   <  SRR4302537.1159959/94‑1 (MQ=57)
                                                                             GGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTC                  <  SRR4302537.2039180/94‑1 (MQ=60)
                                                                             GGGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTC                  <  SRR4302537.1731149/94‑1 (MQ=60)
                                                                              GGGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCA                 <  SRR4302537.2468792/94‑1 (MQ=60)
                                                                               GGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCAT                <  SRR4302537.3566576/94‑1 (MQ=60)
                                                                               GGGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCAT                <  SRR4302537.3840465/94‑1 (MQ=60)
                                                                                GGGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATT               >  SRR4302537.2368322/1‑94 (MQ=60)
                                                                                 GGCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTT              <  SRR4302537.525704/94‑1 (MQ=60)
                                                                                  GCGCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTT             <  SRR4302537.484208/94‑1 (MQ=60)
                                                                                    GCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCT           <  SRR4302537.473181/94‑1 (MQ=60)
                                                                                    GCTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCT           <  SRR4302537.6321973/94‑1 (MQ=60)
                                                                                     CTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTG          <  SRR4302537.1385239/94‑1 (MQ=60)
                                                                                     CTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTG          >  SRR4302537.1199384/1‑94 (MQ=60)
                                                                                     CTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTG          <  SRR4302537.5954170/94‑1 (MQ=60)
                                                                                     CTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTG          >  SRR4302537.1572802/1‑94 (MQ=60)
                                                                                     CTGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTG          >  SRR4302537.534983/1‑94 (MQ=60)
                                                                                      TGGAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTCCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGC         <  SRR4302537.3484066/94‑1 (MQ=60)
                                                                                        GAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAG       <  SRR4302537.5675026/94‑1 (MQ=60)
                                                                                        GAAGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAG       >  SRR4302537.4521371/1‑94 (MQ=60)
                                                                                          AGGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTC     >  SRR4302537.1516908/1‑94 (MQ=60)
                                                                                          AGG‑CATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCT    <  SRR4302537.1603357/94‑1 (MQ=60)
                                                                                           GGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGGACAACTTGAAAACCACTAATTTTCTGCAGTCT    >  SRR4302537.5959936/1‑94 (MQ=60)
                                                                                           GGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCT    <  SRR4302537.2857803/94‑1 (MQ=60)
                                                                                           GGGCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCT    >  SRR4302537.2763774/1‑94 (MQ=60)
                                                                                             GCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGG  <  SRR4302537.3280031/94‑1 (MQ=60)
                                                                                             GCATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGG  <  SRR4302537.6517177/94‑1 (MQ=60)
                                                                                             |                                                                                             
TACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGG  >  NC_000913/4058340‑4058526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑