Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,116,392 G→A intergenic (+52/+33) yiiU → / ← menG hypothetical protein/MenG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,3920GA97.5% 861.8 / 4.1 245intergenic (+52/+33)yiiU/menGhypothetical protein/MenG
Reads supporting (aligned to +/- strand):  ref base G (0/6);  new base A (81/158);  total (81/164)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 249 total aligned reads displayed.

AACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAG  >  NC_000913/4116300‑4116481
                                                                                            |                                                                                         
aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACg                                                                                           <  2:1417476/93‑1 (MQ=255)
  cGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAAcaca                                                                                         <  2:1467582/93‑1 (MQ=255)
      tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGaaa                                                                                     <  1:6302813/93‑1 (MQ=255)
        cAGTCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGaaaaa                                                                                   <  1:1431559/93‑1 (MQ=255)
         aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGaaaaaa                                                                                  <  1:3372635/93‑1 (MQ=255)
         aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGaaaaaa                                                                                  >  2:4722464/1‑93 (MQ=255)
          ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAg                                                                                 <  2:6440019/93‑1 (MQ=255)
            cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGc                                                                               <  1:5358053/93‑1 (MQ=255)
            cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGc                                                                               <  1:4879882/93‑1 (MQ=255)
             ccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCa                                                                              >  2:3755745/1‑93 (MQ=255)
              ctgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCAc                                                                             >  2:3028724/1‑93 (MQ=255)
                gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCt                                                                           <  2:85968/93‑1 (MQ=255)
                gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCt                                                                           <  2:5267320/93‑1 (MQ=255)
                gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCt                                                                           <  2:2890799/93‑1 (MQ=255)
                 ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCtt                                                                          <  1:672289/93‑1 (MQ=255)
                  tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTg                                                                         <  1:3045501/93‑1 (MQ=255)
                   gggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGc                                                                        <  2:3524364/93‑1 (MQ=255)
                    ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCg                                                                       <  1:5331306/93‑1 (MQ=255)
                     gTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCgg                                                                      >  2:1527928/1‑93 (MQ=255)
                       cGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTg                                                                    <  2:4372595/93‑1 (MQ=255)
                        gCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGc                                                                   <  2:2399072/93‑1 (MQ=255)
                         cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGcc                                                                  <  1:4086916/93‑1 (MQ=255)
                          aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTTCCt                                                                 <  1:2598883/93‑1 (MQ=255)
                          aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCt                                                                 <  1:5888510/93‑1 (MQ=255)
                          aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCt                                                                 <  2:2979742/93‑1 (MQ=255)
                             gAAGAGGTCTGATTCCGCTTCATCTCCCGC‑TTATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                                             <  2:3088554/93‑1 (MQ=255)
                             gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTc                                                              >  2:3125021/1‑93 (MQ=255)
                              aaGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                                             <  1:6155497/93‑1 (MQ=255)
                               agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                            >  1:3132235/1‑93 (MQ=255)
                               agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                            >  2:4949719/1‑93 (MQ=255)
                               agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                            <  2:5050098/93‑1 (MQ=255)
                               agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                            >  2:1065295/1‑93 (MQ=255)
                                  ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATc                                                         >  1:4144562/1‑93 (MQ=255)
                                   gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                        <  2:3312535/93‑1 (MQ=255)
                                   gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                        <  2:1286752/93‑1 (MQ=255)
                                    tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                                       <  2:1880044/93‑1 (MQ=255)
                                    tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                                       <  2:6454373/93‑1 (MQ=255)
                                     cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGGCTTTCTTATCAtt                                                      >  1:5636264/1‑93 (MQ=255)
                                     cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                                      <  2:2310012/93‑1 (MQ=255)
                                     cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                                      <  2:1614426/93‑1 (MQ=255)
                                         ttACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                                                  <  1:6422382/93‑1 (MQ=255)
                                         ttACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                                                  <  1:1347158/93‑1 (MQ=255)
                                          tACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAAGGACCCTGCGGGGGCCTTCTTATTATTTAata                                                 >  1:5583512/1‑93 (MQ=255)
                                              cTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                                             <  2:2673191/93‑1 (MQ=255)
                                               ttCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCCCCTTGCGGTGCCTTTCTTATCATTCAATATCCAg                                            <  1:3717187/93‑1 (MQ=255)
                                               ttCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAg                                            >  2:6196930/1‑93 (MQ=255)
                                                tCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                                           <  1:1082125/93‑1 (MQ=255)
                                                tCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                                           <  1:6341809/93‑1 (MQ=255)
                                                tCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                                           >  1:327084/1‑93 (MQ=255)
                                                 cATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                                          <  1:164391/93‑1 (MQ=255)
                                                 cATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                                          >  1:5120524/1‑93 (MQ=255)
                                                  aTCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                                         <  2:5122666/93‑1 (MQ=255)
                                                  aTCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAACGCACCCTGCGGTGGCCTTCTTATCATTCAATATTCAGCgg                                         >  2:5662325/1‑93 (MQ=255)
                                                    cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                                       <  2:59188/93‑1 (MQ=255)
                                                     ttCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                                      <  1:3535311/93‑1 (MQ=255)
                                                      tCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtct                                     <  1:5742952/93‑1 (MQ=255)
                                                         gcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTTTTATCATTCAATATCCAGCGGAtcttct                                  >  1:3555734/1‑93 (MQ=255)
                                                         gcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct                                  <  2:25766/93‑1 (MQ=255)
                                                           gTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGa                                <  1:2865204/93‑1 (MQ=255)
                                                           gTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGa                                <  2:2487274/93‑1 (MQ=255)
                                                             aaTTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATTTTCTGaaa                              <  2:6315791/93‑1 (MQ=255)
                                                              aTTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                             >  1:2512568/1‑93 (MQ=255)
                                                               tttCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTTAATATCCACCGGTTCTTCGAAAAGa                            >  1:3789792/1‑93 (MQ=255)
                                                               tttCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGa                            >  1:998738/1‑93 (MQ=255)
                                                                ttCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa                           >  1:5037549/1‑93 (MQ=255)
                                                                ttCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa                           >  2:769288/1‑93 (MQ=255)
                                                                ttCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa                           <  2:1776413/93‑1 (MQ=255)
                                                                 tCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaat                          <  2:3340732/93‑1 (MQ=255)
                                                                  cTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata                         <  1:3913715/93‑1 (MQ=255)
                                                                   tttACTGTGTAGGCCAACAGGTAACACAGAAAGAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                        <  1:4411276/93‑1 (MQ=255)
                                                                   tttACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                        <  2:2039079/93‑1 (MQ=255)
                                                                    ttACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGAGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat                       >  2:530800/1‑93 (MQ=255)
                                                                       cTGTTTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATccc                    >  1:2807860/1‑93 (MQ=255)
                                                                       cTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATccc                    <  2:6144902/93‑1 (MQ=255)
                                                                       cTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATccc                    >  1:2041205/1‑93 (MQ=255)
                                                                       cTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCCTGCGGTGCCTTTCTTATCATTCAATATACAGCGGATCTTTTGAAAAaataaaacc                    >  1:1200196/1‑89 (MQ=255)
                                                                        tgtgTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                   <  1:627876/93‑1 (MQ=255)
                                                                        tgtgTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCg                   <  2:5421/93‑1 (MQ=255)
                                                                          tgtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGt                 >  2:4292801/1‑93 (MQ=255)
                                                                           gtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                <  2:3989654/93‑1 (MQ=255)
                                                                           gtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                >  1:2976493/1‑93 (MQ=255)
                                                                           gtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                <  1:3379646/93‑1 (MQ=255)
                                                                           gtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                <  2:3075112/93‑1 (MQ=255)
                                                                           gtAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTa                <  2:6089898/93‑1 (MQ=255)
                                                                            tAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAt               <  2:4688456/93‑1 (MQ=255)
                                                                             aGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAtt              <  2:643350/93‑1 (MQ=255)
                                                                              ggCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTg             <  2:957316/93‑1 (MQ=255)
                                                                               gCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGt            <  1:4223285/93‑1 (MQ=255)
                                                                                ccAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTc           >  2:611827/1‑93 (MQ=255)
                                                                                 cAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCg          <  2:2749795/93‑1 (MQ=255)
                                                                                  aaCAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCgg         <  1:5156077/93‑1 (MQ=255)
                                                                                   aCAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGc        >  1:5417549/1‑93 (MQ=255)
                                                                                   aCAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGc        >  2:3728612/1‑93 (MQ=255)
                                                                                   aCAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGc        >  2:4871065/1‑93 (MQ=255)
                                                                                     aGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCAt      <  1:3871495/93‑1 (MQ=255)
                                                                                     aGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAAACCCGGATTTTCGGCAt      >  2:3551316/1‑93 (MQ=255)
                                                                                       gTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATaa    <  1:6045671/93‑1 (MQ=255)
                                                                                        tAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATaaa   <  2:382276/93‑1 (MQ=255)
                                                                                         aaCACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAg  >  2:2991903/1‑93 (MQ=255)
                                                                                         aaCACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAg  <  1:2166908/93‑1 (MQ=255)
                                                                                            |                                                                                         
AACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAG  >  NC_000913/4116300‑4116481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGG  >  NC_000913/4116301‑4116485
                                                                                           |                                                                                             
ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACA                                                                                             <  SRR4302537.3282817/94‑1 (MQ=60)
ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACA                                                                                             >  SRR4302537.3152620/1‑94 (MQ=60)
 CGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAG                                                                                            >  SRR4302537.747918/1‑94 (MQ=60)
  GTCTGCAGGCCCTGCTGTGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGA                                                                                           >  SRR4302537.2620149/1‑94 (MQ=60)
  GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGA                                                                                           <  SRR4302537.5252584/94‑1 (MQ=60)
   TCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAA                                                                                          <  SRR4302537.1131188/94‑1 (MQ=60)
     TGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAA                                                                                        <  SRR4302537.6345977/94‑1 (MQ=60)
       CAGTCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAA                                                                                      <  SRR4302537.1441525/94‑1 (MQ=60)
        AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAG                                                                                     <  SRR4302537.3439269/94‑1 (MQ=60)
        AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAG                                                                                     <  SRR4302537.2575517/94‑1 (MQ=60)
        AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAG                                                                                     <  SRR4302537.3396535/94‑1 (MQ=60)
         GGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGG                                                                                    <  SRR4302537.3700926/94‑1 (MQ=60)
          GCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGC                                                                                   <  SRR4302537.6398538/94‑1 (MQ=60)
          GCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGC                                                                                   <  SRR4302537.6328846/94‑1 (MQ=60)
           CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCA                                                                                  <  SRR4302537.4913591/94‑1 (MQ=60)
           CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCA                                                                                  <  SRR4302537.1406648/94‑1 (MQ=60)
           CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCA                                                                                  <  SRR4302537.138130/94‑1 (MQ=60)
           CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCA                                                                                  <  SRR4302537.5394748/94‑1 (MQ=60)
            CCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCAC                                                                                 >  SRR4302537.4194645/1‑94 (MQ=60)
             ggggggggggggggggaggttttttttgccttttttttttcccgctaAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACC                                                                                <  SRR4302537.5270302/47‑1 (MQ=60)
             CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACC                                                                                <  SRR4302537.6331976/94‑1 (MQ=60)
              TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCT                                                                               <  SRR4302537.4557991/94‑1 (MQ=60)
               GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTT                                                                              <  SRR4302537.6208986/94‑1 (MQ=60)
               GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTT                                                                              <  SRR4302537.6161064/94‑1 (MQ=60)
                CTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTG                                                                             <  SRR4302537.676891/94‑1 (MQ=60)
                 TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGC                                                                            <  SRR4302537.3066714/94‑1 (MQ=60)
                  GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCG                                                                           <  SRR4302537.2833890/94‑1 (MQ=60)
                   GGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGG                                                                          <  SRR4302537.5367878/94‑1 (MQ=60)
                   GGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAAGCACCTTGCGG                                                                          >  SRR4302537.1216231/1‑94 (MQ=60)
                     TCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTG                                                                        <  SRR4302537.3061477/94‑1 (MQ=60)
                        CATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGgggct                                                                     >  SRR4302537.3527651/1‑89 (MQ=60)
                        CATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCT                                                                     <  SRR4302537.4115455/94‑1 (MQ=60)
                         ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTTCCTT                                                                    <  SRR4302537.2617061/94‑1 (MQ=60)
                         ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTT                                                                    >  SRR4302537.4792197/1‑94 (MQ=60)
                         ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTT                                                                    <  SRR4302537.5928913/94‑1 (MQ=60)
                          TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTT                                                                   <  SRR4302537.2756309/94‑1 (MQ=60)
                          TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTT                                                                   <  SRR4302537.5155137/94‑1 (MQ=60)
                            GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                                                 <  SRR4302537.1640073/94‑1 (MQ=60)
                            GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                                                 >  SRR4302537.3177524/1‑94 (MQ=60)
                             AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                                                >  SRR4302537.3154210/1‑94 (MQ=60)
                             AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                                                <  SRR4302537.6197847/94‑1 (MQ=60)
                              AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                                               <  SRR4302537.5835544/94‑1 (MQ=60)
                                AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                                             >  SRR4302537.4173475/1‑94 (MQ=60)
                                AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                                             <  SRR4302537.4017836/94‑1 (MQ=60)
                                 GGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCCTGCGGTGCCTTTCTTATCA                                                            >  SRR4302537.5600624/1‑94 (MQ=60)
                                   TCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGGCTTTCTTATCATT                                                          >  SRR4302537.5674836/1‑94 (MQ=60)
                                    CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                                         <  SRR4302537.6013178/94‑1 (MQ=60)
                                        TTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAaggaccctgcgggggccttcttattatttaata                                                     >  SRR4302537.5621682/1‑61 (MQ=60)
                                        TTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATA                                                     <  SRR4302537.1356548/94‑1 (MQ=60)
                                        TTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATA                                                     <  SRR4302537.6466035/94‑1 (MQ=60)
                                          ACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATC                                                   <  SRR4302537.575634/94‑1 (MQ=60)
                                          ACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATC                                                   <  SRR4302537.6395615/94‑1 (MQ=60)
                                           CGCTGCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCC                                                  <  SRR4302537.5623660/94‑1 (MQ=60)
                                              TTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCCCCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                                               <  SRR4302537.3743315/94‑1 (MQ=60)
                                              TTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                                               >  SRR4302537.329355/1‑94 (MQ=60)
                                               TCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCG                                              <  SRR4302537.1089596/94‑1 (MQ=60)
                                               TCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCG                                              <  SRR4302537.6385138/94‑1 (MQ=60)
                                               TCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCG                                              >  SRR4302537.5155817/1‑94 (MQ=60)
                                               TCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCCTGCGGTGGCTTTCTTATCATTCAATATCCAGCG                                              >  SRR4302537.5828045/1‑94 (MQ=60)
                                                CATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                                             <  SRR4302537.165559/94‑1 (MQ=60)
                                                CATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                                             >  SRR4302537.2956743/1‑94 (MQ=60)
                                                CATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                                             >  SRR4302537.5023040/1‑94 (MQ=60)
                                                CATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                                             <  SRR4302537.2308265/94‑1 (MQ=60)
                                                CAGCTTCCGCGTAATTTCTTTACTGTGTAGGCTAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                                             <  SRR4302537.5373272/94‑1 (MQ=60)
                                                 ATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                                            <  SRR4302537.424690/94‑1 (MQ=60)
                                                 ATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                                            <  SRR4302537.339937/94‑1 (MQ=60)
                                                    TTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                                         <  SRR4302537.3560287/94‑1 (MQ=60)
                                                    TTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                                         <  SRR4302537.1823638/94‑1 (MQ=60)
                                                    TTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                                         <  SRR4302537.3822148/94‑1 (MQ=60)
                                                     TCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTT                                        <  SRR4302537.25026/94‑1 (MQ=60)
                                                     TCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTT                                        <  SRR4302537.5782273/94‑1 (MQ=60)
                                                      CCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGAGGATCTTC                                       >  SRR4302537.3951683/1‑94 (MQ=60)
                                                       CGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTTTTATCATTCAATATCCAGCGGATCTTCT                                      >  SRR4302537.3580830/1‑94 (MQ=60)
                                                         CGTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGA                                    <  SRR4302537.5448232/94‑1 (MQ=60)
                                                          GTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGGA                                   >  SRR4302537.5173143/1‑94 (MQ=60)
                                                          GTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA                                   <  SRR4302537.567314/94‑1 (MQ=60)
                                                          GTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA                                   >  SRR4302537.3932323/1‑94 (MQ=60)
                                                          GTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA                                   <  SRR4302537.3322275/94‑1 (MQ=60)
                                                          GTAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA                                   <  SRR4302537.2885294/94‑1 (MQ=60)
                                                           TAATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTTAATATCCAGCGGATCTTCTGAAA                                  >  SRR4302537.307536/1‑94 (MQ=60)
                                                            AATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG                                 >  SRR4302537.2530234/1‑94 (MQ=60)
                                                            AATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG                                 <  SRR4302537.2039910/94‑1 (MQ=60)
                                                             ATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTTAATATCCACCGGTTCTTCGAAAAGA                                >  SRR4302537.3816391/1‑94 (MQ=60)
                                                             ATTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA                                >  SRR4302537.1005633/1‑94 (MQ=60)
                                                              TTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA                               <  SRR4302537.443841/94‑1 (MQ=60)
                                                              TTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA                               >  SRR4302537.5072228/1‑94 (MQ=60)
                                                              TTTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA                               >  SRR4302537.6363873/1‑94 (MQ=60)
                                                               TTCTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT                              >  SRR4302537.908599/1‑94 (MQ=60)
                                                                 CTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA                            <  SRR4302537.3941075/94‑1 (MQ=60)
                                                                 CTTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA                            <  SRR4302537.2934124/94‑1 (MQ=60)
                                                                  TTTACTGTGTAGGCCAACAGGTAACACAGAAAGAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT                           <  SRR4302537.4441713/94‑1 (MQ=60)
                                                                  TTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT                           >  SRR4302537.3854918/1‑94 (MQ=60)
                                                                  TTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT                           <  SRR4302537.3760402/94‑1 (MQ=60)
                                                                  TTTACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT                           >  SRR4302537.4126143/1‑94 (MQ=60)
                                                                    TACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCC                         >  SRR4302537.509869/1‑94 (MQ=60)
                                                                     ACTGTTTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCC                        >  SRR4302537.2827488/1‑94 (MQ=60)
                                                                     ACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCC                        >  SRR4302537.2055464/1‑94 (MQ=60)
                                                                     ACTGTGTAGGCCAACAGGTAACACAGAAAAAAGGCACCCTGCGGTGCCTTTCTTATCATTCAATATACAGCGGATCTTTTGAAAAAATAAaacc                        >  SRR4302537.1208538/1‑90 (MQ=60)
                                                                       TGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG                      <  SRR4302537.632151/94‑1 (MQ=60)
                                                                         TGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTA                    <  SRR4302537.3540293/94‑1 (MQ=60)
                                                                         TGTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTA                    >  SRR4302537.2997325/1‑94 (MQ=60)
                                                                          GTAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAT                   <  SRR4302537.3403595/94‑1 (MQ=60)
                                                                           TAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATT                  <  SRR4302537.6135331/94‑1 (MQ=60)
                                                                           TAGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATT                  >  SRR4302537.5406149/1‑94 (MQ=60)
                                                                            AGGCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTTTCATTCAATATTCAGCGGATCTTCTTAAAGAATAATACCGGTATTG                 >  SRR4302537.5618898/1‑94 (MQ=60)
                                                                              GCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTC               <  SRR4302537.3328604/94‑1 (MQ=60)
                                                                              GCCAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTC               <  SRR4302537.4252699/94‑1 (MQ=60)
                                                                                CAACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGG             <  SRR4302537.6497028/94‑1 (MQ=60)
                                                                                 AACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGC            >  SRR4302537.5454478/1‑94 (MQ=60)
                                                                                 AACAGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGC            <  SRR4302537.5191609/94‑1 (MQ=60)
                                                                                    AGGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATA         <  SRR4302537.3898586/94‑1 (MQ=60)
                                                                                     GGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAA        <  SRR4302537.356109/94‑1 (MQ=60)
                                                                                     GGTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAA        <  SRR4302537.2161573/94‑1 (MQ=60)
                                                                                      GTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAA       <  SRR4302537.6087191/94‑1 (MQ=60)
                                                                                      GTAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAA       >  SRR4302537.5316710/1‑94 (MQ=60)
                                                                                       TAACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAG      <  SRR4302537.368247/94‑1 (MQ=60)
                                                                                        AACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGA     <  SRR4302537.2182170/94‑1 (MQ=60)
                                                                                         ACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAT    <  SRR4302537.2096997/94‑1 (MQ=60)
                                                                                         ACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAT    <  SRR4302537.2492242/94‑1 (MQ=60)
                                                                                         ACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAT    <  SRR4302537.336555/94‑1 (MQ=60)
                                                                                         ACACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAT    <  SRR4302537.4933402/94‑1 (MQ=60)
                                                                                         ACACAGAAAAAAGCCACCTTGCGGTGCCTTTCTTATCATTCAATGTCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGAT    <  SRR4302537.3969262/94‑1 (MQ=60)
                                                                                          CACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATG   <  SRR4302537.1816839/94‑1 (MQ=60)
                                                                                          CACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATG   <  SRR4302537.1691106/94‑1 (MQ=60)
                                                                                           ACAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGG  <  SRR4302537.2343159/94‑1 (MQ=60)
                                                                                           |                                                                                             
ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGG  >  NC_000913/4116301‑4116485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑