Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,792	C→T	G462G (GGC→GGT)	metH →	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,792	0	C	T	99.3%	923.3 / NA	270	G462G (GGC→GGT)	metH	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (139/128); minor base G (2/0); total (142/128)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

GATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGA  >  NC_000913/4222702‑4222881

gATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGgtgt                                                                                         <  2:5850475/93‑1 (MQ=255)

   caaCTCCTCAAAATGGGACGTCATTGAAAAAGATCTGAAGTGTATGCAGGGAAAAGGCATTGTTATCTCTATCTCGTAGAAAGAGAGTGTCGa                                                                                      <  2:3343316/91‑1 (MQ=255)

   cGACTCCTGAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTACCTCCAACTCGATGAAAGGGGGTGTCGa                                                                                      <  2:237182/93‑1 (MQ=255)

   cGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGa                                                                                      <  2:877666/93‑1 (MQ=255)

   cGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGa                                                                                      <  1:5428914/93‑1 (MQ=255)

    gACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAt                                                                                     <  1:556092/93‑1 (MQ=255)

    gACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAt                                                                                     >  1:1804083/1‑93 (MQ=255)

     aCTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATg                                                                                    >  1:2530731/1‑93 (MQ=255)

       tcctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGcc                                                                                  <  1:3836395/93‑1 (MQ=255)

       tcctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGcc                                                                                  <  1:4155566/93‑1 (MQ=255)

       tcctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGcc                                                                                  <  2:779615/93‑1 (MQ=255)

         ctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCtt                                                                                <  2:3719208/93‑1 (MQ=255)

          tcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCttt                                                                               >  2:3766407/1‑93 (MQ=255)

           cAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGGTGCCTTTa                                                                              <  2:6303256/93‑1 (MQ=255)

           cAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTa                                                                              <  2:160578/93‑1 (MQ=255)

           cAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTa                                                                              >  1:1407435/1‑93 (MQ=255)

                tGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCAt                                                                         >  1:506934/1‑93 (MQ=255)

                 gggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATc                                                                        >  1:2904790/1‑93 (MQ=255)

                 gggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATc                                                                        >  2:2556165/1‑93 (MQ=255)

                 gggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATc                                                                        >  1:5338628/1‑93 (MQ=255)

                  ggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCa                                                                       <  2:2415839/93‑1 (MQ=255)

                  ggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCa                                                                       <  1:2911924/93‑1 (MQ=255)

                    aCGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAcg                                                                     >  2:4775252/1‑93 (MQ=255)

                     cGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAcgc                                                                    >  1:5880256/1‑93 (MQ=255)

                     cGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAcgc                                                                    <  2:483862/93‑1 (MQ=255)

                     cGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAcgc                                                                    >  2:5683726/1‑93 (MQ=255)

                      gTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAcgcg                                                                   >  1:2141492/1‑93 (MQ=255)

                         aTTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCCCGCGaaa                                                                <  2:3521660/93‑1 (MQ=255)

                          ttGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAt                                                               <  2:666459/93‑1 (MQ=255)

                           tGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAtt                                                              <  1:3290872/93‑1 (MQ=255)

                            gAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttg                                                             <  2:5023110/93‑1 (MQ=255)

                            gAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttg                                                             <  2:6454561/93‑1 (MQ=255)

                             aaaaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgt                                                            <  1:6357082/93‑1 (MQ=255)

                             aaaaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgt                                                            >  2:3116152/1‑93 (MQ=255)

                             aaaaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgt                                                            <  2:5767385/93‑1 (MQ=255)

                              aaaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgtt                                                           >  1:5797665/1‑93 (MQ=255)

                               aaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgttg                                                          <  2:5193217/93‑1 (MQ=255)

                               aaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAttgttg                                                          >  2:5630690/1‑93 (MQ=255)

                                aaGGTCTGAAGTGGATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGc                                                         <  2:3801698/93‑1 (MQ=255)

                                 aGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCg                                                        <  1:3514054/93‑1 (MQ=255)

                                 aGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCg                                                        <  2:2948801/93‑1 (MQ=255)

                                  ggTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGt                                                       >  2:2883901/1‑93 (MQ=255)

                                  ggTCGGAAGTGTATCAAGGGCAAAGGCATAGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCGTCACGCGAAATTGTTGCGt                                                       <  2:5943384/93‑1 (MQ=255)

                                    tCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCg                                                     >  2:2301659/1‑93 (MQ=255)

                                      tGAAGTGTATCCAGGGCAAAGGCATTTTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCt                                                   <  2:4562223/93‑1 (MQ=255)

                                      tGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCt                                                   >  1:3117131/1‑93 (MQ=255)

                                       gAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTa                                                  <  1:568607/93‑1 (MQ=255)

                                       gAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTa                                                  >  1:1422930/1‑93 (MQ=255)

                                       gAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTa                                                  >  2:2278970/1‑93 (MQ=255)

                                        aaGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTAc                                                 <  1:2636254/93‑1 (MQ=255)

                                        aaGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTAc                                                 <  2:3847139/93‑1 (MQ=255)

                                         aGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACg                                                >  1:215890/1‑93 (MQ=255)

                                           tgtATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGt                                              <  1:608529/93‑1 (MQ=255)

                                           tgtATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGt                                              <  1:3014279/93‑1 (MQ=255)

                                            gtATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTg                                             >  2:4632655/1‑93 (MQ=255)

                                            gtATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTg                                             <  2:2196830/93‑1 (MQ=255)

                                             tATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGc                                            <  2:5477002/93‑1 (MQ=255)

                                             tATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGc                                            <  2:4661134/93‑1 (MQ=255)

                                              aTCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCg                                           <  2:3545113/93‑1 (MQ=255)

                                              aTCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCg                                           >  2:4970784/1‑93 (MQ=255)

                                               tCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCgg                                          >  2:1012204/1‑93 (MQ=255)

                                                 cAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCa                                        >  2:4447476/1‑93 (MQ=255)

                                                   gggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgt                                      >  1:320234/1‑93 (MQ=255)

                                                   gggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgg                                      >  2:1120771/1‑92 (MQ=255)

                                                    ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtg                                     >  1:3952233/1‑93 (MQ=255)

                                                      cAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtggt                                   >  1:933382/1‑93 (MQ=255)

                                                      cAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtggt                                   >  1:5605818/1‑93 (MQ=255)

                                                       aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtggtg                                  >  2:5138117/1‑93 (MQ=255)

                                                       aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtggtg                                  >  1:1130002/1‑93 (MQ=255)

                                                       aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAgtggtg                                  >  1:3598393/1‑93 (MQ=255)

                                                       aaaGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCCGCAgtggtg                                  >  1:5204589/1‑93 (MQ=255)

                                                          ggCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTa                               >  1:1547605/1‑93 (MQ=255)

                                                           gCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTaa                              <  2:1946114/93‑1 (MQ=255)

                                                           gCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTaa                              >  1:4488908/1‑93 (MQ=255)

                                                           gCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTaa                              <  1:5000612/93‑1 (MQ=255)

                                                           gCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTaa                              >  2:4874477/1‑93 (MQ=255)

                                                            cATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAAt                             <  1:5941395/93‑1 (MQ=255)

                                                             aTTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATg                            <  1:3171675/93‑1 (MQ=255)

                                                              ttGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATgg                           >  1:2461893/1‑93 (MQ=255)

                                                                 ttAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCt                        >  1:539840/1‑93 (MQ=255)

                                                                 ttAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCt                        >  1:6442945/1‑93 (MQ=255)

                                                                  tAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCtt                       <  1:408386/93‑1 (MQ=255)

                                                                   aaCTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCttt                      >  1:6194085/1‑93 (MQ=255)

                                                                   aaCTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCttt                      <  1:2784331/93‑1 (MQ=255)

                                                                    aCTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTg                     >  1:6428124/1‑93 (MQ=255)

                                                                     ctctATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGa                    <  1:1068210/93‑1 (MQ=255)

                                                                      tctATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAc                   <  1:1309779/93‑1 (MQ=255)

                                                                      tctATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAc                   >  1:78024/1‑93 (MQ=255)

                                                                      tctATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAc                   >  1:2643479/1‑93 (MQ=255)

                                                                       ctATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACg                  <  2:3248163/93‑1 (MQ=255)

                                                                        tATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGa                 >  2:539516/1‑93 (MQ=255)

                                                                         aTCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGGGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGaa                <  1:3240838/93‑1 (MQ=255)

                                                                         aTCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGaa                <  2:6150377/93‑1 (MQ=255)

                                                                          tctcGATGAAAGAGGGTGTCGATGCGTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGacc               <  1:6249140/93‑3 (MQ=255)

                                                                          tctcGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAAc               <  2:2053634/93‑1 (MQ=255)

                                                                           ctcGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACa              <  1:426098/93‑1 (MQ=255)

                                                                            tcGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAg             <  2:5590290/93‑1 (MQ=255)

                                                                                tGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGAc         >  2:4207457/1‑93 (MQ=255)

                                                                                   aaGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAgg      >  1:2310333/1‑93 (MQ=255)

                                                                                       gggTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGa  >  1:2671704/1‑93 (MQ=255)

GATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGA  >  NC_000913/4222702‑4222881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACT  >  NC_000913/4222699‑4222885

TATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGT                                                                                               <  SRR4302537.4656681/94‑1 (MQ=60)

 ATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTACCTCTATCTCGATGAAAGAGGGTG                                                                                              <  SRR4302537.1447148/94‑1 (MQ=60)

 ATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTG                                                                                              >  SRR4302537.2942232/1‑94 (MQ=60)

 ATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTG                                                                                              >  SRR4302537.188689/1‑94 (MQ=60)

  TGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGT                                                                                             <  SRR4302537.927601/94‑1 (MQ=60)

  TGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGT                                                                                             <  SRR4302537.2316107/94‑1 (MQ=60)

    ATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCG                                                                                           >  SRR4302537.1432462/1‑94 (MQ=60)

      CGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAT                                                                                         <  SRR4302537.4227579/94‑1 (MQ=60)

      CGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAT                                                                                         <  SRR4302537.5465932/94‑1 (MQ=60)

      CGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAT                                                                                         >  SRR4302537.1816674/1‑94 (MQ=60)

      CGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGAT                                                                                         >  SRR4302537.1377322/1‑94 (MQ=60)

       GACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATG                                                                                        <  SRR4302537.559918/94‑1 (MQ=60)

       GACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATG                                                                                        >  SRR4302537.2548488/1‑94 (MQ=60)

       GACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATG                                                                                        <  SRR4302537.2159269/94‑1 (MQ=60)

          TCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCT                                                                                     <  SRR4302537.4184586/94‑1 (MQ=60)

          TCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCT                                                                                     <  SRR4302537.3863277/94‑1 (MQ=60)

            CTCAAAATGGGACGTAATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTT                                                                                   >  SRR4302537.3322816/1‑94 (MQ=60)

             TCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTA                                                                                  >  SRR4302537.1417235/1‑94 (MQ=60)

              CAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTAT                                                                                 <  SRR4302537.5400792/94‑1 (MQ=60)

               AAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATC                                                                                <  SRR4302537.2595801/94‑1 (MQ=60)

                  ATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCAT                                                                             >  SRR4302537.510413/1‑94 (MQ=60)

                  ATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCAT                                                                             >  SRR4302537.2545070/1‑94 (MQ=60)

                   TGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATC                                                                            >  SRR4302537.3191434/1‑94 (MQ=60)

                   TGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATC                                                                            >  SRR4302537.2925195/1‑94 (MQ=60)

                   TGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATC                                                                            >  SRR4302537.5375224/1‑94 (MQ=60)

                    GGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCA                                                                           <  SRR4302537.1609044/94‑1 (MQ=60)

                    GGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCA                                                                           <  SRR4302537.4446396/94‑1 (MQ=60)

                     GGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAC                                                                          <  SRR4302537.2932380/94‑1 (MQ=60)

                     GGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCAC                                                                          <  SRR4302537.4011852/94‑1 (MQ=60)

                      GACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACG                                                                         <  SRR4302537.2160728/94‑1 (MQ=60)

                       ACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGC                                                                        >  SRR4302537.5920598/1‑94 (MQ=60)

                        CGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCG                                                                       <  SRR4302537.2248270/94‑1 (MQ=60)

                        CGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCG                                                                       >  SRR4302537.2156563/1‑94 (MQ=60)

                        CGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCG                                                                       <  SRR4302537.2105239/94‑1 (MQ=60)

                        CGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCG                                                                       <  SRR4302537.5684778/94‑1 (MQ=60)

                          TCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAA                                                                     >  SRR4302537.1110003/1‑94 (MQ=60)

                            ATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAT                                                                   >  SRR4302537.5954894/1‑94 (MQ=60)

                            ATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAT                                                                   >  SRR4302537.5620682/1‑94 (MQ=60)

                            ATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAT                                                                   <  SRR4302537.5650866/94‑1 (MQ=60)

                            ATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAAT                                                                   >  SRR4302537.3700261/1‑94 (MQ=60)

                             TTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATT                                                                  <  SRR4302537.2971879/94‑1 (MQ=60)

                             TTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATT                                                                  <  SRR4302537.417089/94‑1 (MQ=60)

                             TTGAAAAAGGGCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGGGTCGATGCCTTTATCCATCACGCGAAATT                                                                  >  SRR4302537.3233674/1‑94 (MQ=60)

                              TGAAAAAGGTCTGAAGTGTATCCAGGGCCAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTG                                                                 <  SRR4302537.6259087/94‑1 (MQ=60)

                              TGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTG                                                                 <  SRR4302537.3314126/94‑1 (MQ=60)

                              TGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTG                                                                 >  SRR4302537.2145356/1‑94 (MQ=60)

                              TGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTG                                                                 >  SRR4302537.4685593/1‑94 (MQ=60)

                                AAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTT                                                               <  SRR4302537.6400469/94‑1 (MQ=60)

                                AAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTT                                                               >  SRR4302537.5837412/1‑94 (MQ=60)

                                AAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTT                                                               <  SRR4302537.5174831/94‑1 (MQ=60)

                                AAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTT                                                               >  SRR4302537.1420553/1‑94 (MQ=60)

                                 AAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTG                                                              <  SRR4302537.714168/94‑1 (MQ=60)

                                 AAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTG                                                              <  SRR4302537.2675479/94‑1 (MQ=60)

                                 AAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTAACCATCACGCGAAATTGTTG                                                              >  SRR4302537.4064080/1‑94 (MQ=60)

                                  AAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGC                                                             >  SRR4302537.3217040/1‑94 (MQ=60)

                                   AAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCG                                                            >  SRR4302537.5480489/1‑94 (MQ=60)

                                   AAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCG                                                            >  SRR4302537.3603372/1‑94 (MQ=60)

                                    AGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGT                                                           <  SRR4302537.3538915/94‑1 (MQ=60)

                                       TCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGC                                                        <  SRR4302537.5622971/94‑1 (MQ=60)

                                        CTGAAGTGTATCCAGGGCAAAGGCATTGTTACCTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCT                                                       <  SRR4302537.285418/94‑1 (MQ=60)

                                        CTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTCTCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCT                                                       >  SRR4302537.2681967/1‑94 (MQ=60)

                                        CTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCT                                                       >  SRR4302537.3138902/1‑94 (MQ=60)

                                         TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTA                                                      >  SRR4302537.1432839/1‑94 (MQ=60)

                                         TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTA                                                      <  SRR4302537.285238/94‑1 (MQ=60)

                                         TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTA                                                      >  SRR4302537.1644610/1‑94 (MQ=60)

                                          GAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTAC                                                     <  SRR4302537.572517/94‑1 (MQ=60)

                                           AAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACG                                                    >  SRR4302537.217432/1‑94 (MQ=60)

                                           AAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACG                                                    <  SRR4302537.5003696/94‑1 (MQ=60)

                                           AAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACG                                                    <  SRR4302537.6207025/94‑1 (MQ=60)

                                           AAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACG                                                    <  SRR4302537.2654700/94‑1 (MQ=60)

                                            AGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGG                                                   >  SRR4302537.4237427/1‑94 (MQ=60)

                                              TGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTG                                                 >  SRR4302537.2701465/1‑94 (MQ=60)

                                              TGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTG                                                 <  SRR4302537.612688/94‑1 (MQ=60)

                                              TGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTG                                                 <  SRR4302537.3035295/94‑1 (MQ=60)

                                               GTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGC                                                >  SRR4302537.5800650/1‑94 (MQ=60)

                                                TATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCG                                               <  SRR4302537.529455/94‑1 (MQ=60)

                                                 ATCCAGGGCAAAGGCATTGTTAACTCTATCTCGCTGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGG                                              >  SRR4302537.826337/1‑94 (MQ=60)

                                                  TCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGC                                             <  SRR4302537.3192293/94‑1 (MQ=60)

                                                   CCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCA                                            <  SRR4302537.5041323/94‑1 (MQ=60)

                                                   CCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCA                                            >  SRR4302537.6338102/1‑94 (MQ=60)

                                                    CAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAG                                           <  SRR4302537.6439142/94‑1 (MQ=60)

                                                     AGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGT                                          >  SRR4302537.536024/1‑94 (MQ=60)

                                                     AGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGT                                          >  SRR4302537.322454/1‑94 (MQ=60)

                                                     AGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGG                                          >  SRR4302537.1357957/1‑94 (MQ=60)

                                                      GGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTG                                         >  SRR4302537.1293225/1‑94 (MQ=60)

                                                      GGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTG                                         >  SRR4302537.3979877/1‑94 (MQ=60)

                                                       GGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGG                                        <  SRR4302537.5451352/94‑1 (MQ=60)

                                                        GCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGT                                       >  SRR4302537.5644144/1‑94 (MQ=60)

                                                        GCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGT                                       >  SRR4302537.939814/1‑94 (MQ=60)

                                                         CAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTATGGTGCGGCAGTGGTG                                      >  SRR4302537.5974028/1‑94 (MQ=60)

                                                         CAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTG                                      >  SRR4302537.1137842/1‑94 (MQ=60)

                                                         CAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTG                                      >  SRR4302537.3623756/1‑94 (MQ=60)

                                                         CAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCCGCAGTGGTG                                      >  SRR4302537.5240391/1‑94 (MQ=60)

                                                         CAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCAGCAGTGGTG                                      <  SRR4302537.6481810/94‑1 (MQ=60)

                                                          AAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGG                                     >  SRR4302537.100205/1‑94 (MQ=60)

                                                          AAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGG                                     >  SRR4302537.6430482/1‑94 (MQ=60)

                                                            AGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTA                                   >  SRR4302537.1558407/1‑94 (MQ=60)

                                                            AGGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTA                                   >  SRR4302537.6501338/1‑94 (MQ=60)

                                                             GGCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAA                                  >  SRR4302537.4519960/1‑94 (MQ=60)

                                                              GCATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAAT                                 <  SRR4302537.5035062/94‑1 (MQ=60)

                                                               CATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATG                                <  SRR4302537.1196813/94‑1 (MQ=60)

                                                               CATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATG                                >  SRR4302537.6393587/1‑94 (MQ=60)

                                                               CATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATG                                <  SRR4302537.5982175/94‑1 (MQ=60)

                                                               CATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATG                                >  SRR4302537.2541607/1‑94 (MQ=60)

                                                               CATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATG                                <  SRR4302537.3237472/94‑1 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGGCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               >  SRR4302537.2659637/1‑94 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               >  SRR4302537.2365022/1‑94 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               >  SRR4302537.2479208/1‑94 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               <  SRR4302537.3193959/94‑1 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               <  SRR4302537.5845782/94‑1 (MQ=60)

                                                                ATTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGG                               >  SRR4302537.5887418/1‑94 (MQ=60)

                                                                 TTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGC                              <  SRR4302537.5122233/94‑1 (MQ=60)

                                                                 TTGTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGC                              <  SRR4302537.815090/94‑1 (MQ=60)

                                                                   GTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCT                            >  SRR4302537.543556/1‑94 (MQ=60)

                                                                   GTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCT                            <  SRR4302537.2701198/94‑1 (MQ=60)

                                                                   GTTAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCT                            >  SRR4302537.6486663/1‑94 (MQ=60)

                                                                     TAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTT                          >  SRR4302537.3053576/1‑94 (MQ=60)

                                                                     TAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTT                          >  SRR4302537.6236767/1‑94 (MQ=60)

                                                                     TAACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTT                          <  SRR4302537.411215/94‑1 (MQ=60)

                                                                      AACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTG                         <  SRR4302537.2803794/94‑1 (MQ=60)

                                                                      AACTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTG                         >  SRR4302537.6471802/1‑94 (MQ=60)

                                                                        CTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAC                       >  SRR4302537.2661972/1‑94 (MQ=60)

                                                                        CTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAC                       >  SRR4302537.78595/1‑94 (MQ=60)

                                                                        CTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGAC                       <  SRR4302537.1075572/94‑1 (MQ=60)

                                                                        CTCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGGGGTAATGGCCTTTGAC                       >  SRR4302537.2931682/1‑94 (MQ=60)

                                                                         TCTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACG                      <  SRR4302537.1318896/94‑1 (MQ=60)

                                                                          CTATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGA                     >  SRR4302537.5556922/1‑94 (MQ=60)

                                                                            ATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGGGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAAC                   <  SRR4302537.3263679/94‑1 (MQ=60)

                                                                            ATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAAC                   >  SRR4302537.3942620/1‑94 (MQ=60)

                                                                            ATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTCACGAAA                   >  SRR4302537.542855/1‑94 (MQ=60)

                                                                            ATCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCCCTACGGTGCGGCAGTGGCGGTAATGGCCTTTGACGAAC                   >  SRR4302537.3281960/1‑94 (MQ=60)

                                                                             TCTCGATGAAAGAGGGTGTCGATGCGTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGACCA                  <  SRR4302537.6292072/94‑1 (MQ=60)

                                                                             TCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACA                  <  SRR4302537.3469075/94‑1 (MQ=60)

                                                                             TCTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACA                  <  SRR4302537.627524/94‑1 (MQ=60)

                                                                              CTCGATGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAG                 <  SRR4302537.429057/94‑1 (MQ=60)

                                                                                   TGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACA            >  SRR4302537.6122639/1‑94 (MQ=60)

                                                                                   TGAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACA            >  SRR4302537.4400005/1‑94 (MQ=60)

                                                                                    GAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAG           >  SRR4302537.2456868/1‑94 (MQ=60)

                                                                                    GAAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAG           <  SRR4302537.326449/94‑1 (MQ=60)

                                                                                    GAAAGAGGGGGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAG           >  SRR4302537.2557151/1‑94 (MQ=60)

                                                                                     AAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGG          >  SRR4302537.2326641/1‑94 (MQ=60)

                                                                                     AAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGG          <  SRR4302537.606119/94‑1 (MQ=60)

                                                                                     AAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGG          >  SRR4302537.3072106/1‑94 (MQ=60)

                                                                                     AAAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGG          <  SRR4302537.3743205/94‑1 (MQ=60)

                                                                                      AAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGC         >  SRR4302537.3888333/1‑94 (MQ=60)

                                                                                      AAGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGC         >  SRR4302537.1001419/1‑94 (MQ=60)

                                                                                       AGAGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCC        >  SRR4302537.299117/1‑94 (MQ=60)

                                                                                         AGGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGA      >  SRR4302537.2690368/1‑94 (MQ=60)

                                                                                          GGGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGAT     <  SRR4302537.1552546/94‑1 (MQ=60)

                                                                                           GGTGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATA    >  SRR4302537.2121975/1‑94 (MQ=60)

                                                                                             TGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACT  <  SRR4302537.3244517/94‑1 (MQ=60)

TATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACT  >  NC_000913/4222699‑4222885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`