Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,253,662 C→T M128I (ATG→ATA)  plsB ← glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,253,6620CT100.0% 890.7 / NA 253M128I (ATG→ATA) plsBglycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (95/158);  total (95/158)

Only 100 of 278 total aligned reads displayed.

CCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTC  >  NC_000913/4253572‑4253751
                                                                                          |                                                                                         
ccACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAatat                                                                                         >  1:5148166/1‑93 (MQ=255)
 cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATc                                                                                        >  1:4276385/1‑93 (MQ=255)
 cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGCTCACTTCGCCTTTTTCTCTCCCCGGCGCGCGACCAAAAATc                                                                                        >  1:3683143/1‑93 (MQ=255)
  acaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCTACCAAATATCa                                                                                       <  2:2186053/93‑1 (MQ=255)
  acaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCa                                                                                       >  2:1990272/1‑93 (MQ=255)
    aGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCAcc                                                                                     >  2:1809397/1‑93 (MQ=255)
      tACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGa                                                                                   <  2:5827842/93‑1 (MQ=255)
       aCAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGac                                                                                  <  2:4582764/93‑1 (MQ=255)
        cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTTCACGCCCCGGCGCGCGGCGAAATATCACCGacc                                                                                 >  1:1442518/1‑92 (MQ=255)
         aGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGacac                                                                                <  1:3775298/93‑1 (MQ=255)
           cGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTg                                                                              <  2:559731/93‑1 (MQ=255)
           cGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTg                                                                              <  2:244187/93‑1 (MQ=255)
           cGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTg                                                                              <  1:2828017/93‑1 (MQ=255)
            gAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTgg                                                                             >  2:1745385/1‑93 (MQ=255)
            gAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTCACGCCCCCGCGCGCGACCAAATATCACCGacacccg                                                                             >  1:703140/1‑90 (MQ=255)
             aaaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGc                                                                            <  1:2139242/93‑1 (MQ=255)
             aaaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCAAATATCACCGACCCTgga                                                                            >  1:5652731/1‑92 (MQ=255)
              aaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAATATCACCGACACTGGCa                                                                           >  1:2711748/1‑93 (MQ=255)
                aaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCAcc                                                                         <  1:4525529/93‑1 (MQ=255)
                aaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCAcc                                                                         >  1:4204885/1‑93 (MQ=255)
                  tttCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCAt                                                                       >  1:153173/1‑93 (MQ=255)
                   ttCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATc                                                                      <  2:1402883/93‑1 (MQ=255)
                      tGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGc                                                                   >  2:5151399/1‑93 (MQ=255)
                         aCGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGAGCGACCAAATATCACCGACACTGGCACCATCTGcaca                                                                >  1:3021531/1‑93 (MQ=255)
                           gCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATc                                                              <  2:3791521/93‑1 (MQ=255)
                             cGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCCTCTGCACATCCa                                                            >  1:4496154/1‑93 (MQ=255)
                             cGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCa                                                            <  2:305296/93‑1 (MQ=255)
                             cGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACCGGCACCATCTGCACATTCa                                                            >  1:6082730/1‑93 (MQ=255)
                               ttAATCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAGATATCACCGACACTGGCACCATCTGCACATCCAGa                                                          <  2:2364414/93‑1 (MQ=255)
                                tAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGAt                                                         <  2:837814/93‑1 (MQ=255)
                                  aGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGAttt                                                       <  2:3118853/93‑1 (MQ=255)
                                  aGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGAttt                                                       <  2:3602304/93‑1 (MQ=255)
                                    cATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCCAATATCACCGACACTGGCACCATCTGCACATCCAGAtttcg                                                     >  2:542972/1‑91 (MQ=255)
                                     aTACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTggg                                                    >  1:3169714/1‑93 (MQ=255)
                                      tACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGt                                                   <  2:5009196/93‑1 (MQ=255)
                                      tACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGt                                                   <  2:1154504/93‑1 (MQ=255)
                                       aCGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGtt                                                  <  2:3409999/93‑1 (MQ=255)
                                         gCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGc                                                <  1:4413807/93‑1 (MQ=255)
                                           aGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTa                                              <  2:6237316/93‑1 (MQ=255)
                                            gcggcgGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAc                                             <  1:3081801/93‑1 (MQ=255)
                                             cggcggGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACg                                            <  2:2899248/93‑1 (MQ=255)
                                               gcggGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGt                                          <  1:5742703/93‑1 (MQ=255)
                                               gcggGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGGTGCTACggg                                          >  2:5469606/1‑92 (MQ=255)
                                                 gggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc                                        <  1:5403539/93‑1 (MQ=255)
                                                 gggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc                                        >  1:4577485/1‑93 (MQ=255)
                                                 gggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc                                        <  2:3239958/93‑1 (MQ=255)
                                                  ggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCa                                       >  1:3405641/1‑93 (MQ=255)
                                                     tCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAt                                    <  1:1883172/93‑1 (MQ=255)
                                                     tCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAt                                    <  1:862700/93‑1 (MQ=255)
                                                      cACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc                                   <  2:5293950/93‑1 (MQ=255)
                                                      cACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc                                   >  1:64587/1‑93 (MQ=255)
                                                      cACTTCGCCTTTGTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc                                   <  1:2743046/93‑1 (MQ=255)
                                                        cTTCTCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga                                 <  2:1408344/93‑1 (MQ=255)
                                                        cTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCTCCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATAga                                 >  2:1902764/1‑93 (MQ=255)
                                                        cTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga                                 >  2:4784001/1‑93 (MQ=255)
                                                         ttCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCgag                                >  1:3634396/1‑93 (MQ=255)
                                                          tCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCgaga                               >  2:4274702/1‑93 (MQ=255)
                                                           cGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                              <  2:6707/93‑1 (MQ=255)
                                                            gCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATa                             <  2:6398498/93‑1 (MQ=255)
                                                            gCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATa                             >  2:4898018/1‑93 (MQ=255)
                                                            gCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTCGGTTGCTACGGTGCAAATCGAGATa                             >  1:3986501/1‑93 (MQ=255)
                                                             ccTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAg                            <  1:4074303/93‑1 (MQ=255)
                                                              cTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                           >  1:1318950/1‑93 (MQ=255)
                                                              cTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                           <  1:349732/93‑1 (MQ=255)
                                                              cTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                           <  2:366275/93‑1 (MQ=255)
                                                              cTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                           >  2:393210/1‑93 (MQ=255)
                                                              cTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                           <  2:6285389/93‑1 (MQ=255)
                                                               tttttCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTc                          >  2:1139363/1‑93 (MQ=255)
                                                               tttttCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTc                          <  2:4679324/93‑1 (MQ=255)
                                                               tttttCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTc                          >  1:2363495/1‑93 (MQ=255)
                                                                ttttCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCg                         <  1:4948026/93‑1 (MQ=255)
                                                                ttttCACGCCACGGCGCGCGACAAAATATCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCg                         <  2:3492543/93‑1 (MQ=255)
                                                                 tttCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGt                        <  1:2515919/93‑1 (MQ=255)
                                                                   tCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTgg                      <  2:4583930/93‑1 (MQ=255)
                                                                     aCGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                    <  1:2551893/93‑1 (MQ=255)
                                                                       gCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACa                  <  1:1052700/93‑1 (MQ=255)
                                                                        ccccGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAg                 <  2:923544/93‑1 (MQ=255)
                                                                          ccGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCt               <  2:5893292/93‑1 (MQ=255)
                                                                           cGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt              >  2:4219583/1‑93 (MQ=255)
                                                                           cGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCtt              <  2:6027121/93‑1 (MQ=255)
                                                                            ggCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTa             >  2:210379/1‑93 (MQ=255)
                                                                             tcgcgcgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa            >  1:746198/2‑93 (MQ=255)
                                                                             gcgcgcGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTaa            <  1:6367147/93‑1 (MQ=255)
                                                                              cgcgcgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAAt           <  1:6332091/93‑1 (MQ=255)
                                                                               gcgcgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATa          >  2:2615395/1‑93 (MQ=255)
                                                                               gcgcgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATa          <  2:5734123/93‑1 (MQ=255)
                                                                               gcgcgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATa          <  2:452525/93‑1 (MQ=255)
                                                                                  cgACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc       <  1:5936724/93‑1 (MQ=255)
                                                                                   gACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAct      <  1:3074317/93‑1 (MQ=255)
                                                                                   gACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAct      <  1:1240647/93‑1 (MQ=255)
                                                                                    aCCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc     >  2:5255689/1‑93 (MQ=255)
                                                                                    aCCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc     <  2:3820587/93‑1 (MQ=255)
                                                                                    aCCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc     >  1:1658433/1‑93 (MQ=255)
                                                                                    aCCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc     >  1:1501200/1‑93 (MQ=255)
                                                                                     ccAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActct    >  1:5261514/1‑93 (MQ=255)
                                                                                     ccAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActct    <  2:598136/93‑1 (MQ=255)
                                                                                      cAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt   >  2:4033543/1‑93 (MQ=255)
                                                                                      cAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt   >  1:3863598/1‑93 (MQ=255)
                                                                                      cAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt   >  1:2238544/1‑93 (MQ=255)
                                                                                       aaaTATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc  <  2:1606964/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTC  >  NC_000913/4253572‑4253751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTC  >  NC_000913/4253570‑4253755
                                                                                            |                                                                                             
AGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATA                                                                                              >  SRR4302537.636790/1‑94 (MQ=60)
 GCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATAT                                                                                             <  SRR4302537.1009928/94‑1 (MQ=60)
 GCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATAT                                                                                             <  SRR4302537.2123523/94‑1 (MQ=60)
 GCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATAT                                                                                             <  SRR4302537.217211/94‑1 (MQ=60)
 GCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATAT                                                                                             <  SRR4302537.3858255/94‑1 (MQ=60)
 GCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATAT                                                                                             >  SRR4302537.5183642/1‑94 (MQ=60)
  CCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATC                                                                                            >  SRR4302537.4306104/1‑94 (MQ=60)
  CCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGCTCACTTCGCCTTTTTCTCTCCCCGGCGCGCGACCAAAAATC                                                                                            >  SRR4302537.3709044/1‑94 (MQ=60)
      AGTACAGCGAAAAATTTCTGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCG                                                                                        <  SRR4302537.3676797/94‑1 (MQ=60)
      AGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTGCCGCCCCGGCGCGCGACCAAATATCACCG                                                                                        <  SRR4302537.1944479/94‑1 (MQ=60)
       GTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGA                                                                                       <  SRR4302537.3473574/94‑1 (MQ=60)
         ACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTTCACGCCCCGGCGCGCGGCGAAATATCACCGACC                                                                                     >  SRR4302537.1452568/1‑94 (MQ=60)
           AGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACT                                                                                   <  SRR4302537.3801806/94‑1 (MQ=60)
             CGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGG                                                                                 <  SRR4302537.2847778/94‑1 (MQ=60)
             CGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGG                                                                                 <  SRR4302537.6381856/94‑1 (MQ=60)
             CGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACAATGG                                                                                 >  SRR4302537.2653413/1‑94 (MQ=60)
             CGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTCACGCCCCCGCGCGCGACCAAATATCACCGACACccg                                                                                 >  SRR4302537.707952/1‑91 (MQ=60)
              GAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGC                                                                                >  SRR4302537.6335017/1‑94 (MQ=60)
              GAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCAAATATCACCGACCCTGGA                                                                                >  SRR4302537.5691415/1‑94 (MQ=60)
               AAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCA                                                                               <  SRR4302537.2154301/94‑1 (MQ=60)
               AAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAATATCACCGACACTGGCA                                                                               >  SRR4302537.2730688/1‑94 (MQ=60)
                AAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCAC                                                                              >  SRR4302537.519714/1‑94 (MQ=60)
                 AAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACC                                                                             >  SRR4302537.4234233/1‑94 (MQ=60)
                  AATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCA                                                                            <  SRR4302537.4556843/94‑1 (MQ=60)
                   ATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCAT                                                                           <  SRR4302537.5449438/94‑1 (MQ=60)
                   ATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCAT                                                                           >  SRR4302537.154265/1‑94 (MQ=60)
                   ATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCAT                                                                           >  SRR4302537.571495/1‑94 (MQ=60)
                   ATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCAT                                                                           >  SRR4302537.2555429/1‑94 (MQ=60)
                     TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCT                                                                         <  SRR4302537.6319259/94‑1 (MQ=60)
                      TCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTG                                                                        <  SRR4302537.1537916/94‑1 (MQ=60)
                        TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCC                                                                      >  SRR4302537.2821724/1‑94 (MQ=60)
                        TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCA                                                                      >  SRR4302537.3521947/1‑94 (MQ=60)
                         GTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCAC                                                                     <  SRR4302537.4533655/94‑1 (MQ=60)
                         GTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGACTTTTTCACGCCCCGGCGAGCGACCAAATATTATCGACACTGGCACCATCTGCAC                                                                     >  SRR4302537.647735/1‑94 (MQ=60)
                          TACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGAGCGACCAAATATCACCGACACTGGCACCATCTGCACA                                                                    >  SRR4302537.3042595/1‑94 (MQ=60)
                           ACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACAT                                                                   <  SRR4302537.1378419/94‑1 (MQ=60)
                           ACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACAT                                                                   <  SRR4302537.458672/94‑1 (MQ=60)
                             GCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCC                                                                 >  SRR4302537.513844/1‑94 (MQ=60)
                             GCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCC                                                                 <  SRR4302537.4828429/94‑1 (MQ=60)
                              CCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCCTCTGCACATCCA                                                                >  SRR4302537.4527256/1‑94 (MQ=60)
                              CCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCA                                                                >  SRR4302537.4014854/1‑94 (MQ=60)
                              CCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCA                                                                <  SRR4302537.4841256/94‑1 (MQ=60)
                              CCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTCGCACCATCTGCACATCCA                                                                >  SRR4302537.2569573/1‑94 (MQ=60)
                              CCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACCGGCACCATCTGCACATTCA                                                                >  SRR4302537.6124526/1‑94 (MQ=60)
                               CGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAG                                                               >  SRR4302537.3785159/1‑94 (MQ=60)
                               CGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAG                                                               >  SRR4302537.3098018/1‑94 (MQ=60)
                               CGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAG                                                               <  SRR4302537.2431967/94‑1 (MQ=60)
                               CGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCAACGACACTGGCACCATCTGCACATCCAG                                                               >  SRR4302537.2632524/1‑94 (MQ=60)
                                 TTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACCCTGGCAACATCTGCACATCCAGAT                                                             >  SRR4302537.410629/1‑94 (MQ=60)
                                    AGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTG                                                          <  SRR4302537.3334438/94‑1 (MQ=60)
                                      CATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGG                                                        >  SRR4302537.3191984/1‑94 (MQ=60)
                                      CATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGG                                                        >  SRR4302537.4291502/1‑94 (MQ=60)
                                      CATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGG                                                        >  SRR4302537.5280734/1‑94 (MQ=60)
                                       ATACGCAGCGGCGGGTTTACTTCGCCTTTTTCACGCCCCTGCGCGCGGCCCAATATCACCGCCACTGGCACCAACTGCACATCCAAATTTGGGT                                                       >  SRR4302537.2345593/1‑94 (MQ=60)
                                        TACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTT                                                      <  SRR4302537.5329629/94‑1 (MQ=60)
                                           GCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT                                                   <  SRR4302537.4444270/94‑1 (MQ=60)
                                            CAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTA                                                  <  SRR4302537.4002343/94‑1 (MQ=60)
                                             ggcggcggtttccctccgctttttcccccCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAC                                                 <  SRR4302537.1281506/65‑1 (MQ=60)
                                              GCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACG                                                <  SRR4302537.3103266/94‑1 (MQ=60)
                                               CGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGG                                               >  SRR4302537.4519955/1‑94 (MQ=60)
                                                GGCGGGTTCACTTCGCCTTTTTTACGCCCCGGCGCGCGGCCAAAAATCACCGCCACTGGCACCCTCTGCACATGCAGATTTGGGTTGCTtctgg                                              >  SRR4302537.2761025/1‑89 (MQ=60)
                                                 GCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTG                                             <  SRR4302537.5782023/94‑1 (MQ=60)
                                                  CGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC                                            >  SRR4302537.4609163/1‑94 (MQ=60)
                                                   GGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCA                                           <  SRR4302537.5440409/94‑1 (MQ=60)
                                                   GGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCA                                           >  SRR4302537.3429761/1‑94 (MQ=60)
                                                    GGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAA                                          <  SRR4302537.1669197/94‑1 (MQ=60)
                                                     GTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAA                                         >  SRR4302537.682263/1‑94 (MQ=60)
                                                      TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT                                        <  SRR4302537.5467154/94‑1 (MQ=60)
                                                       TCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC                                       >  SRR4302537.65065/1‑94 (MQ=60)
                                                       TCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC                                       <  SRR4302537.868616/94‑1 (MQ=60)
                                                       TCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC                                       <  SRR4302537.1896267/94‑1 (MQ=60)
                                                        CACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCG                                      <  SRR4302537.3849680/94‑1 (MQ=60)
                                                        CACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCG                                      >  SRR4302537.1919/1‑94 (MQ=60)
                                                        CACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCG                                      >  SRR4302537.593780/1‑94 (MQ=60)
                                                        CACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCG                                      <  SRR4302537.2749450/94‑1 (MQ=60)
                                                        CACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATAG                                      <  SRR4302537.37581/94‑1 (MQ=60)
                                                        CACTTCGCCTTTGTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCG                                      <  SRR4302537.2762182/94‑1 (MQ=60)
                                                          CTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG                                    >  SRR4302537.3659997/1‑94 (MQ=60)
                                                          CTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG                                    <  SRR4302537.5717044/94‑1 (MQ=60)
                                                           TTCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGA                                   <  SRR4302537.6486252/94‑1 (MQ=60)
                                                            TCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGAT                                  <  SRR4302537.6397499/94‑1 (MQ=60)
                                                            TCGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGAT                                  >  SRR4302537.3080930/1‑94 (MQ=60)
                                                             CGCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTCGGTTGCTACGGTGCAAATCGAGATA                                 >  SRR4302537.4014416/1‑94 (MQ=60)
                                                              GCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAG                                >  SRR4302537.1684350/1‑94 (MQ=60)
                                                              GCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAG                                >  SRR4302537.4441605/1‑94 (MQ=60)
                                                              GCCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAG                                <  SRR4302537.2984927/94‑1 (MQ=60)
                                                               CCTTTTTCGCGCCCCGGCGCGCGACCAAATAGCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGT                               <  SRR4302537.880509/94‑1 (MQ=60)
                                                               CCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGT                               <  SRR4302537.4102751/94‑1 (MQ=60)
                                                               CCTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGT                               >  SRR4302537.1328144/1‑94 (MQ=60)
                                                               CCTTTTTAACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGT                               >  SRR4302537.1252623/1‑94 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              >  SRR4302537.303739/1‑94 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              >  SRR4302537.2380161/1‑94 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              <  SRR4302537.3252954/94‑1 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              <  SRR4302537.352167/94‑1 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              <  SRR4302537.4248092/94‑1 (MQ=60)
                                                                CTTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTC                              >  SRR4302537.4057166/1‑94 (MQ=60)
                                                                 TTTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCG                             <  SRR4302537.3641657/94‑1 (MQ=60)
                                                                  TTTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGT                            <  SRR4302537.4982138/94‑1 (MQ=60)
                                                                   TTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTG                           <  SRR4302537.112296/94‑1 (MQ=60)
                                                                   TTTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTG                           <  SRR4302537.2533599/94‑1 (MQ=60)
                                                                    TTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGG                          <  SRR4302537.220359/94‑1 (MQ=60)
                                                                    TTCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAATCGTGG                          >  SRR4302537.708505/1‑94 (MQ=60)
                                                                     TCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGA                         <  SRR4302537.4595477/94‑1 (MQ=60)
                                                                     TCACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGA                         >  SRR4302537.1485186/1‑94 (MQ=60)
                                                                      CACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAA                        <  SRR4302537.3614368/94‑1 (MQ=60)
                                                                      CACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAA                        <  SRR4302537.4467329/94‑1 (MQ=60)
                                                                       ACGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAAC                       <  SRR4302537.2569786/94‑1 (MQ=60)
                                                                        CGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACA                      <  SRR4302537.3099352/94‑1 (MQ=60)
                                                                        CGCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACA                      <  SRR4302537.3056464/94‑1 (MQ=60)
                                                                         GCCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAG                     <  SRR4302537.1059961/94‑1 (MQ=60)
                                                                          CCCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGC                    >  SRR4302537.1743508/1‑94 (MQ=60)
                                                                           CCCGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCT                   >  SRR4302537.5115731/1‑94 (MQ=60)
                                                                             CGGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCTCATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTA                 <  SRR4302537.2242394/94‑1 (MQ=60)
                                                                              GTCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAA                >  SRR4302537.751289/1‑94 (MQ=60)
                                                                              GGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAA                >  SRR4302537.2780840/1‑94 (MQ=60)
                                                                              GGCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAA                <  SRR4302537.3882215/94‑1 (MQ=60)
                                                                               GCGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAAT               <  SRR4302537.6410571/94‑1 (MQ=60)
                                                                                CGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATA              <  SRR4302537.6375392/94‑1 (MQ=60)
                                                                                CGCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATA              <  SRR4302537.6123196/94‑1 (MQ=60)
                                                                                 GCGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAG             <  SRR4302537.3176005/94‑1 (MQ=60)
                                                                                  CGCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGA            >  SRR4302537.2315214/1‑94 (MQ=60)
                                                                                   GCGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAC           <  SRR4302537.1607791/94‑1 (MQ=60)
                                                                                    CGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACT          <  SRR4302537.394628/94‑1 (MQ=60)
                                                                                    CGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACT          <  SRR4302537.856698/94‑1 (MQ=60)
                                                                                    CGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACT          >  SRR4302537.146753/1‑94 (MQ=60)
                                                                                    CGACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACT          <  SRR4302537.5977468/94‑1 (MQ=60)
                                                                                     GACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTC         >  SRR4302537.1670056/1‑94 (MQ=60)
                                                                                     GACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTC         >  SRR4302537.1511680/1‑94 (MQ=60)
                                                                                     GACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTC         <  SRR4302537.3095737/94‑1 (MQ=60)
                                                                                     GACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTC         <  SRR4302537.1249271/94‑1 (MQ=60)
                                                                                     GACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTC         >  SRR4302537.687305/1‑94 (MQ=60)
                                                                                      ACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCT        >  SRR4302537.5297714/1‑94 (MQ=60)
                                                                                      ACCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCT        <  SRR4302537.837701/94‑1 (MQ=60)
                                                                                       CCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTT       <  SRR4302537.6498723/94‑1 (MQ=60)
                                                                                       CCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTT       >  SRR4302537.3890646/1‑94 (MQ=60)
                                                                                       CCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTT       <  SRR4302537.1346105/94‑1 (MQ=60)
                                                                                       CCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTT       <  SRR4302537.2441217/94‑1 (MQ=60)
                                                                                       CCAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTT       >  SRR4302537.2254329/1‑94 (MQ=60)
                                                                                        CAAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTC      <  SRR4302537.5818539/94‑1 (MQ=60)
                                                                                         AAATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCT     >  SRR4302537.3750488/1‑94 (MQ=60)
                                                                                          AATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTT    <  SRR4302537.4695902/94‑1 (MQ=60)
                                                                                           ATATCACCGACACTGGCACTATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTT   <  SRR4302537.4613013/94‑1 (MQ=60)
                                                                                           ATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTT   <  SRR4302537.6100950/94‑1 (MQ=60)
                                                                                           ATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTT   <  SRR4302537.2605897/94‑1 (MQ=60)
                                                                                           ATATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTT   >  SRR4302537.5289705/1‑94 (MQ=60)
                                                                                            TATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTC  <  SRR4302537.5130139/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTC  >  NC_000913/4253570‑4253755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑