Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,296	C→T	A396T (GCG→ACG)	yjcG ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,296	0	C	T	100.0%	644.4 / NA	181	A396T (GCG→ACG)	yjcG	putative transport protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (124/57); total (124/57)

Only 100 of 214 total aligned reads displayed.

GATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGG  >  NC_000913/4281206‑4281385

gATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCgtgc                                                                                          >  2:1090048/1‑93 (MQ=255)

gATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCgtgc                                                                                          >  2:5405450/1‑93 (MQ=255)

 aTTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGcc                                                                                         <  1:2883331/93‑1 (MQ=255)

 aTTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGcc                                                                                         <  1:3901818/93‑1 (MQ=255)

  ttCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCt                                                                                        <  2:2945423/93‑1 (MQ=255)

  ttCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCt                                                                                        >  1:1962988/1‑93 (MQ=255)

   tctcAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCtt                                                                                       <  2:636261/93‑1 (MQ=255)

    ctcAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCttt                                                                                      <  1:5822499/93‑1 (MQ=255)

     tcAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCtttt                                                                                     >  1:592891/1‑93 (MQ=255)

     tcAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCtttt                                                                                     <  2:490480/93‑1 (MQ=255)

     tcAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCtttt                                                                                     >  2:5897746/1‑93 (MQ=255)

      cAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCttttt                                                                                    <  1:2980235/93‑1 (MQ=255)

      cAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCttttt                                                                                    <  1:3532294/93‑1 (MQ=255)

      cAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCttttt                                                                                    <  2:3470066/93‑1 (MQ=255)

       aaaCAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCtttttt                                                                                   <  1:6308697/93‑1 (MQ=255)

        aaCAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTg                                                                                  >  2:2828320/1‑93 (MQ=255)

         aCAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGa                                                                                 <  2:4309268/93‑1 (MQ=255)

            gCACGCCGAGGATAATCGCAATCACGCCGAGTATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGa                                                                              >  2:928337/1‑93 (MQ=255)

               cGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGt                                                                           >  2:2022226/1‑93 (MQ=255)

                gCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGtt                                                                          <  1:5384962/93‑1 (MQ=255)

                gCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGtt                                                                          <  2:5862967/93‑1 (MQ=255)

                    aGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCg                                                                      >  1:1665692/1‑93 (MQ=255)

                     ggATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCACGTATTCACGTTCCGTCGTGACGTTTTTGAGGACGATAGCgc                                                                     >  2:4743372/1‑92 (MQ=255)

                      gATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTa                                                                    <  2:2431532/93‑1 (MQ=255)

                        tAATCGCACTCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACa                                                                  >  2:6294660/1‑93 (MQ=255)

                        tAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACa                                                                  >  1:4540004/1‑93 (MQ=255)

                         aaTCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACaa                                                                 <  1:6022944/93‑1 (MQ=255)

                         aaTCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACaa                                                                 >  1:2315538/1‑93 (MQ=255)

                          aTCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAg                                                                >  1:2933573/1‑93 (MQ=255)

                          aTCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAg                                                                >  1:2302615/1‑93 (MQ=255)

                             gCAGTCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCa                                                             <  2:5447539/93‑1 (MQ=255)

                             gCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCa                                                             >  2:6204998/1‑93 (MQ=255)

                             gCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGACTGTTTTGAAGACGTTAGCGTACAAGTCa                                                             >  2:3832498/1‑93 (MQ=255)

                              cAATCACGCCTAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCGTTTTTGAAGACGTTAGCGTACAAGTCAt                                                            >  2:1579508/1‑93 (MQ=255)

                               aaTCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATg                                                           >  2:3424846/1‑93 (MQ=255)

                                aTCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGc                                                          >  2:3889531/1‑93 (MQ=255)

                                   aCGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGaa                                                       >  1:1659626/1‑93 (MQ=255)

                                    cGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGaaa                                                      >  1:1000083/1‑93 (MQ=255)

                                    cGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGaaa                                                      >  1:1075770/1‑93 (MQ=255)

                                      ccGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAAcc                                                    <  2:5840685/93‑1 (MQ=255)

                                       cGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACcg                                                   <  1:4192625/93‑1 (MQ=255)

                                         aGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGACACcgcg                                                 >  1:3116475/1‑93 (MQ=255)

                                         aGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACcgcg                                                 <  2:6372142/93‑1 (MQ=255)

                                          ggATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCgg                                                >  1:5635338/1‑93 (MQ=255)

                                           gATCATGACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGa                                               <  1:890320/93‑1 (MQ=255)

                                           gATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGa                                               <  1:1421888/93‑1 (MQ=255)

                                           gATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGa                                               <  1:3626919/93‑1 (MQ=255)

                                            aTCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAt                                              >  1:1788842/1‑93 (MQ=255)

                                             tCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATg                                             >  1:5215177/1‑93 (MQ=255)

                                               aGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGCTgcg                                           >  2:138457/1‑93 (MQ=255)

                                               aGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATgcg                                           >  2:718719/1‑93 (MQ=255)

                                                gTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATgcg‑c                                         <  2:1186596/93‑1 (MQ=255)

                                                gTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATgcg‑c                                         >  1:6341341/1‑93 (MQ=255)

                                                gTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATgcg‑c                                         >  2:2207309/1‑93 (MQ=255)

                                                  aCGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGcg‑ccc                                       >  2:2649134/1‑93 (MQ=255)

                                                  aCGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGcg‑ccc                                       >  2:1100830/1‑93 (MQ=255)

                                                    ggTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGCTGcg‑cccgc                                     >  2:3231103/1‑93 (MQ=255)

                                                    ggTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGcg‑cccgc                                     >  1:3435124/1‑93 (MQ=255)

                                                       gATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCGTGCGAAACCGCGGATGCG‑CCCGCCAg                                  >  2:5219295/1‑93 (MQ=255)

                                                         tttttGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCg                                >  2:3515411/1‑93 (MQ=255)

                                                         tttttGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCg                                >  2:4735987/1‑93 (MQ=255)

                                                          ttttGATCCCCGCATCTCTTCACGTCCGTTCGTGCTTTTTTGGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCC‑CCCGCCAGCGt                               <  2:3744340/93‑1 (MQ=255)

                                                            ttGATCCCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCa                             <  2:2564254/93‑1 (MQ=255)

                                                            ttGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCa                             >  2:5015599/1‑93 (MQ=255)

                                                            ttGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCa                             >  1:6421995/1‑93 (MQ=255)

                                                             tGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAg                            >  1:2873913/1‑93 (MQ=255)

                                                              gATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGa                           >  2:5749142/1‑93 (MQ=255)

                                                              aaTACCCGCACCTCTTCACTTTCGGTCGTGCTTTTTTTAAAGACGTTAGCGTCCAAGTCATGCAAAACCGCGGTTGCG‑CCCGCCAGCGTCAGa                           <  2:3720065/92‑1 (MQ=255)

                                                               aTACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGAc                          >  2:4363715/1‑93 (MQ=255)

                                                               aTACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGAc                          >  1:139082/1‑93 (MQ=255)

                                                                tccccGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGAcc                         <  1:3326966/91‑1 (MQ=255)

                                                                tccccGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGAcc                         <  2:4683099/91‑1 (MQ=255)

                                                                   ccGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGc                      >  1:1064074/1‑93 (MQ=255)

                                                                   ccGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGc                      >  2:2344797/1‑93 (MQ=255)

                                                                    cGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCa                     >  1:838173/1‑93 (MQ=255)

                                                                     gCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCaa                    >  1:1715045/1‑93 (MQ=255)

                                                                      cAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAac                   <  1:6401991/93‑1 (MQ=255)

                                                                       aGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAacc                  >  2:1704549/1‑93 (MQ=255)

                                                                         ttctTCGCGTGCGGTCGTGCCTTTTTTGAAGACTTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccac                <  2:2981667/92‑1 (MQ=255)

                                                                         ctctTCACGTTCGGTCGTTCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCAAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccac                <  2:4614930/93‑1 (MQ=255)

                                                                         ctctTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccac                >  2:1302780/1‑93 (MQ=255)

                                                                         ctctTCACGTTCGGTCGTGCCTTTTTGGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccac                <  1:3233771/93‑1 (MQ=255)

                                                                          tctTCGCGTTGGGTCGTCCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccacc               <  2:1559055/93‑1 (MQ=255)

                                                                           ctTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAaccaccc              >  1:6353496/1‑92 (MQ=255)

                                                                             tCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACcgcg            >  1:2563453/1‑93 (MQ=255)

                                                                              cACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGa           <  2:4156031/93‑1 (MQ=255)

                                                                              cACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGa           >  2:1507294/1‑93 (MQ=255)

                                                                               aCGTTCGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAg          <  1:2400551/93‑1 (MQ=255)

                                                                               aCGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAg          >  2:1816367/1‑93 (MQ=255)

                                                                                 gTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGa        >  2:6051304/1‑93 (MQ=255)

                                                                                  tgcGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGAGGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGAt       <  1:4551646/91‑1 (MQ=255)

                                                                                   tCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATa      >  1:1201698/1‑93 (MQ=255)

                                                                                    cGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAg     <  2:1641553/93‑1 (MQ=255)

                                                                                    cGGTCGTGCCTTTTTGGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAg     <  1:2687838/93‑1 (MQ=255)

                                                                                    cGGTCGTCCTTTTTTTGAGGCCGTGAGACGACAGGTAATGCGAACCCGGGCATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAg     <  2:148138/93‑1 (MQ=255)

                                                                                     gttCGTGCTTTTTTTAAAGACTTTACCGTACAGGTCATGCAAAGCCGCGAAGGCC‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGt    <  1:4029421/91‑1 (MQ=255)

                                                                                     ggTCGTGCCTTTTTTGAGGACGTTAGCGTACAAGCCATACGAAACCGCGGA‑GCGCCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGt    <  1:2184262/93‑1 (MQ=255)

                                                                                     ggTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGCTAgg    >  1:4854840/1‑92 (MQ=255)

                                                                                      gTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGGG‑CCCGCCAGGGCCAAACCCGCAACCACCCCGAGGTTAGTg   >  2:5518556/1‑93 (MQ=255)

                                                                                      gTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTg   >  1:2370032/1‑93 (MQ=255)

GATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGG  >  NC_000913/4281206‑4281385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAA  >  NC_000913/4281203‑4281389

TCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGT                                                                                                <  SRR4302537.5739046/94‑1 (MQ=60)

 CTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTG                                                                                               >  SRR4302537.3898003/1‑94 (MQ=60)

 CTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTG                                                                                               >  SRR4302537.5796748/1‑94 (MQ=60)

  TGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGC                                                                                              >  SRR4302537.4193987/1‑94 (MQ=60)

    ATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCT                                                                                            <  SRR4302537.2903566/94‑1 (MQ=60)

    ATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCT                                                                                            <  SRR4302537.3929095/94‑1 (MQ=60)

    ATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCT                                                                                            >  SRR4302537.1976636/1‑94 (MQ=60)

     TTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTT                                                                                           >  SRR4302537.3023487/1‑94 (MQ=60)

       CTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTT                                                                                         >  SRR4302537.596947/1‑94 (MQ=60)

       CTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTT                                                                                         >  SRR4302537.6360947/1‑94 (MQ=60)

       CTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTT                                                                                         <  SRR4302537.5862402/94‑1 (MQ=60)

         CAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTT                                                                                       <  SRR4302537.3557256/94‑1 (MQ=60)

         CAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTT                                                                                       <  SRR4302537.3001083/94‑1 (MQ=60)

         CAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGGACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTT                                                                                       >  SRR4302537.4578276/1‑94 (MQ=60)

          AAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTG                                                                                      <  SRR4302537.6351892/94‑1 (MQ=60)

          AAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTG                                                                                      >  SRR4302537.233001/1‑94 (MQ=60)

           AACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGA                                                                                     >  SRR4302537.6414342/1‑94 (MQ=60)

               GCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGAC                                                                                 <  SRR4302537.1406056/94‑1 (MQ=60)

                CACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACG                                                                                <  SRR4302537.1296211/94‑1 (MQ=60)

                CACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACG                                                                                >  SRR4302537.2784112/1‑94 (MQ=60)

                CACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACG                                                                                <  SRR4302537.3910258/94‑1 (MQ=60)

                   GCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTA                                                                             <  SRR4302537.5421778/94‑1 (MQ=60)

                     CGAGTATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGC                                                                           <  SRR4302537.1673368/94‑1 (MQ=60)

                     CGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGC                                                                           <  SRR4302537.1075433/94‑1 (MQ=60)

                      GAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCG                                                                          <  SRR4302537.5584939/94‑1 (MQ=60)

                      GAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCG                                                                          >  SRR4302537.1677362/1‑94 (MQ=60)

                         GATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTAC                                                                       <  SRR4302537.2390561/94‑1 (MQ=60)

                         GATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTAC                                                                       >  SRR4302537.3601107/1‑94 (MQ=60)

                          ATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACA                                                                      >  SRR4302537.4571429/1‑94 (MQ=60)

                           TAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAA                                                                     >  SRR4302537.2331884/1‑94 (MQ=60)

                            AATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAG                                                                    <  SRR4302537.6064324/94‑1 (MQ=60)

                            AATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAG                                                                    >  SRR4302537.4450333/1‑94 (MQ=60)

                            AATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAG                                                                    >  SRR4302537.2318869/1‑94 (MQ=60)

                            AATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAG                                                                    >  SRR4302537.2954174/1‑94 (MQ=60)

                              TCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTC                                                                  >  SRR4302537.3973520/1‑94 (MQ=60)

                              TCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTC                                                                  <  SRR4302537.4822762/94‑1 (MQ=60)

                                GCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCAT                                                                <  SRR4302537.2926281/94‑1 (MQ=60)

                                GCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCAT                                                                >  SRR4302537.63901/1‑94 (MQ=60)

                                  AATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGC                                                              >  SRR4302537.6059950/1‑94 (MQ=60)

                                   ATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCG                                                             <  SRR4302537.3075027/94‑1 (MQ=60)

                                     CACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAA                                                           >  SRR4302537.1671260/1‑94 (MQ=60)

                                     CACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAA                                                           >  SRR4302537.2964281/1‑94 (MQ=60)

                                      ACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAA                                                          >  SRR4302537.1083181/1‑94 (MQ=60)

                                      ACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAA                                                          >  SRR4302537.1006983/1‑94 (MQ=60)

                                       CGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAAC                                                         <  SRR4302537.1069348/94‑1 (MQ=60)

                                          CGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGC                                                      >  SRR4302537.872192/1‑94 (MQ=60)

                                          CGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGC                                                      <  SRR4302537.4221909/94‑1 (MQ=60)

                                           GAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGACACCGCG                                                     >  SRR4302537.3138239/1‑94 (MQ=60)

                                           GAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCG                                                     >  SRR4302537.5550362/1‑94 (MQ=60)

                                           GAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCG                                                     >  SRR4302537.1697121/1‑94 (MQ=60)

                                            AGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGG                                                    >  SRR4302537.5673903/1‑94 (MQ=60)

                                             GGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGA                                                   >  SRR4302537.1894457/1‑94 (MQ=60)

                                              GATCATGACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  <  SRR4302537.896448/94‑1 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGTAT                                                  <  SRR4302537.2346199/94‑1 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  <  SRR4302537.1431789/94‑1 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  >  SRR4302537.5923381/1‑94 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  <  SRR4302537.3652484/94‑1 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  <  SRR4302537.2403480/94‑1 (MQ=60)

                                              GATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGAT                                                  >  SRR4302537.1801339/1‑94 (MQ=60)

                                               ATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATG                                                 >  SRR4302537.5251038/1‑94 (MQ=60)

                                                  AGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑C                                             >  SRR4302537.6384669/1‑94 (MQ=60)

                                                  AGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑C                                             >  SRR4302537.2655807/1‑94 (MQ=60)

                                                    TACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCC                                           <  SRR4302537.1139960/94‑1 (MQ=60)

                                                     ACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCG                                          <  SRR4302537.1712320/94‑1 (MQ=60)

                                                     ACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCG                                          >  SRR4302537.3951195/1‑94 (MQ=60)

                                                      CGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGC                                         >  SRR4302537.3459437/1‑94 (MQ=60)

                                                         TGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAG                                      <  SRR4302537.2290308/94‑1 (MQ=60)

                                                           ATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCG                                    >  SRR4302537.3366114/1‑94 (MQ=60)

                                                             TTTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCAAACCCGCGGATGCG‑CCCGCCAGCGTC                                  <  SRR4302537.849553/94‑1 (MQ=60)

                                                              TTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCA                                 >  SRR4302537.73051/1‑94 (MQ=60)

                                                              TTTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCA                                 >  SRR4302537.6465647/1‑94 (MQ=60)

                                                               TTGATCCCCGCAGCTCTCCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAG                                <  SRR4302537.4696730/94‑1 (MQ=60)

                                                               TTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAG                                >  SRR4302537.2894082/1‑94 (MQ=60)

                                                               TTGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCCCGGATGCG‑CCCGCCAGCGTCAG                                <  SRR4302537.6464470/94‑1 (MQ=60)

                                                                TGATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGA                               >  SRR4302537.5266527/1‑94 (MQ=60)

                                                                 GATACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGAC                              >  SRR4302537.140075/1‑94 (MQ=60)

                                                                   tcccccacGCTCTTCACGTTCGGTCGTTCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCC                            <  SRR4302537.2065928/86‑1 (MQ=60)

                                                                   acCCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTAATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCC                            <  SRR4302537.4115587/92‑1 (MQ=60)

                                                                   TCCCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCC                            <  SRR4302537.3350509/94‑1 (MQ=60)

                                                                    ACCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCG                           >  SRR4302537.2189962/1‑94 (MQ=60)

                                                                     CCCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGC                          >  SRR4302537.1071405/1‑94 (MQ=60)

                                                                      CCGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCA                         >  SRR4302537.843915/1‑94 (MQ=60)

                                                                       CGCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAA                        >  SRR4302537.1727021/1‑94 (MQ=60)

                                                                        GCAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAAC                       >  SRR4302537.5805103/1‑94 (MQ=60)

                                                                         cagcctttccCGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCAAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACC                      <  SRR4302537.1512356/84‑1 (MQ=60)

                                                                         CAGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACC                      <  SRR4302537.6445570/94‑1 (MQ=60)

                                                                          AGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCA                     >  SRR4302537.3477333/1‑94 (MQ=60)

                                                                          AGCTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCA                     >  SRR4302537.327091/1‑94 (MQ=60)

                                                                            CTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACC                   <  SRR4302537.1654980/94‑1 (MQ=60)

                                                                            CTCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACC                   >  SRR4302537.2902159/1‑94 (MQ=60)

                                                                            CTCTTCACGTTCGGTCGTGCCTTTTTGGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACC                   <  SRR4302537.3256562/94‑1 (MQ=60)

                                                                             TCTTCACGTTCGGTCGTGCTTTTTTGGAGGCCGTTAGCGTACAAGTCATGCGAACCGGCGGATGCC‑CCCGCCAGCGTCAGACCCGCAACCACCG                  <  SRR4302537.584471/94‑1 (MQ=60)

                                                                             TCTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCC                  >  SRR4302537.6396868/1‑94 (MQ=60)

                                                                             TCTCCACGTTCGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCAAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCG                  <  SRR4302537.4529053/94‑1 (MQ=60)

                                                                              CTTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGC                 <  SRR4302537.5176029/94‑1 (MQ=60)

                                                                               TTCACGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCG                >  SRR4302537.2581411/1‑94 (MQ=60)

                                                                                  ACGTTGGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGG             <  SRR4302537.2484832/94‑1 (MQ=60)

                                                                                  ACGTTCGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGG             <  SRR4302537.2417458/94‑1 (MQ=60)

                                                                                   CGTTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGA            >  SRR4302537.4955197/1‑94 (MQ=60)

                                                                                     TTCGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATA          >  SRR4302537.1210054/1‑94 (MQ=60)

                                                                                     TGCGGTCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGAGGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATA          <  SRR4302537.4583142/94‑1 (MQ=60)

                                                                                       CGGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGctagg        >  SRR4302537.4888335/1‑89 (MQ=60)

                                                                                       CGGTCGTGCCTTTTTGGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGT        <  SRR4302537.2706595/94‑1 (MQ=60)

                                                                                        GGTCGTGCCTTTTTTGAGGACGTTAGCGTACAAGCCATACGAAACCGCGGA‑GCGCCCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTG       <  SRR4302537.2199655/94‑1 (MQ=60)

                                                                                        GGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTG       >  SRR4302537.6322815/1‑94 (MQ=60)

                                                                                        GGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTG       >  SRR4302537.1756913/1‑94 (MQ=60)

                                                                                        GGTCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTG       >  SRR4302537.2386734/1‑94 (MQ=60)

                                                                                          TCGTGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGC     <  SRR4302537.6417819/94‑1 (MQ=60)

                                                                                          TCGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGC     >  SRR4302537.5946819/1‑94 (MQ=60)

                                                                                           CGTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCTAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCG    >  SRR4302537.2753674/1‑94 (MQ=60)

                                                                                           CGGGCTTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAACCCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCG    <  SRR4302537.3704123/94‑1 (MQ=60)

                                                                                            GTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGA   >  SRR4302537.3565495/1‑94 (MQ=60)

                                                                                            GTGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGA   >  SRR4302537.836965/1‑94 (MQ=60)

                                                                                             TGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAA  >  SRR4302537.5759727/1‑94 (MQ=60)

TCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTTCGGTCGCGCCTTTTTTGAAGACGTTAGCGTACAAGTCATGCGAAACCGCGGATGCG‑CCCGCCAGCGTCAGACCCGCAACCACCGCGAGGATAGTGGCGAA  >  NC_000913/4281203‑4281389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`