Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,156	A→G	E11G (GAG→GGG)	ytfK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,156	0	A	G	99.6%	935.6 / ‑4.6	269	E11G (GAG→GGG)	ytfK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (145/122); total (146/123)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.03e-01

Only 100 of 279 total aligned reads displayed.

CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGG  >  NC_000913/4437066‑4437245

ccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCGGATTGTGGCACCGCACAGGCGTAATACTCAGCAGggga                                                                                         <  2:5648769/93‑1 (MQ=255)

ccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGggga                                                                                         >  2:432596/1‑93 (MQ=255)

ccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGggga                                                                                         >  1:5084611/1‑93 (MQ=255)

ccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGggga                                                                                         <  1:3371107/93‑1 (MQ=255)

ccGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGggga                                                                                         <  1:6012119/93‑1 (MQ=255)

 cGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAt                                                                                        >  1:3279449/1‑93 (MQ=255)

 cGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAt                                                                                        <  1:3579612/93‑1 (MQ=255)

 cGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAt                                                                                        <  1:1842585/93‑1 (MQ=255)

 cGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAt                                                                                        >  2:2433344/1‑93 (MQ=255)

  ggATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGAACCGCACAGGCGTAATACTCAGCAGGGGATa                                                                                       >  1:1359191/1‑93 (MQ=255)

     tAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACa                                                                                    >  2:2523182/1‑93 (MQ=255)

        tCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATg                                                                                 >  2:3685496/1‑93 (MQ=255)

         cTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGa                                                                                >  1:1703616/1‑93 (MQ=255)

          tataGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGaa                                                                               >  1:793120/1‑93 (MQ=255)

           ataGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGaaa                                                                              >  2:6075625/1‑93 (MQ=255)

            taGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGaaaa                                                                             >  2:3930911/1‑93 (MQ=255)

            taGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGaaaa                                                                             >  2:2031447/1‑93 (MQ=255)

              gTCACTAAGCATTAAAATTTGCGCCTCCTAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAAtt                                                                           >  2:3197098/1‑93 (MQ=255)

               tCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATACCAATGAAAAttt                                                                          <  1:1528621/93‑1 (MQ=255)

               tCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAAttt                                                                          >  1:1229440/1‑93 (MQ=255)

                 aCTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTc                                                                        <  1:2871841/93‑1 (MQ=255)

                 aCTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTc                                                                        >  2:5979950/1‑93 (MQ=255)

                  cTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTcc                                                                       >  2:333813/1‑93 (MQ=255)

                  cTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTcc                                                                       >  2:346140/1‑93 (MQ=255)

                  cTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTcc                                                                       <  1:6100131/93‑1 (MQ=255)

                   tAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCa                                                                      <  1:2813585/93‑1 (MQ=255)

                   tAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCa                                                                      >  1:6396943/1‑93 (MQ=255)

                   tAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCa                                                                      >  2:2145952/1‑93 (MQ=255)

                    aaGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTccga                                                                     <  1:3200501/93‑3 (MQ=255)

                    aaGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCaa                                                                     >  2:4617207/1‑93 (MQ=255)

                      gCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACg                                                                   >  1:5431558/1‑93 (MQ=255)

                       cATTAAAATTTGCGCCTCATAATAGTTGGTCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACgg                                                                  <  1:2645291/93‑1 (MQ=255)

                       cATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACgg                                                                  >  2:5505862/1‑93 (MQ=255)

                        aTTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGt                                                                 >  2:3064801/1‑93 (MQ=255)

                        aTTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGt                                                                 >  1:4077771/1‑93 (MQ=255)

                         ttAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTa                                                                >  1:6224463/1‑93 (MQ=255)

                          tAAAATTTTCGCCTCATAATAGTTGGGCCGATTGTGGCACCACACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTAc                                                               >  2:6277629/1‑93 (MQ=255)

                          tAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTAc                                                               <  2:5812278/93‑1 (MQ=255)

                           aaaaTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACa                                                              >  1:1496712/1‑93 (MQ=255)

                               tttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAAccc                                                          >  2:709396/1‑93 (MQ=255)

                               tttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAAccc                                                          >  1:3649906/1‑93 (MQ=255)

                               tttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAAccc                                                          <  1:52530/93‑1 (MQ=255)

                                ttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCa                                                         <  2:5438948/93‑1 (MQ=255)

                                ttGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCa                                                         <  2:4095704/93‑1 (MQ=255)

                                 tGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCAc                                                        >  1:4714263/1‑93 (MQ=255)

                                 tGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCAc                                                        <  1:4473028/93‑1 (MQ=255)

                                  gcgcCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACt                                                       <  2:6382983/93‑1 (MQ=255)

                                   cgcCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACtt                                                      >  2:1026671/1‑93 (MQ=255)

                                    gcCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAGTTTTCCAACGGTACAACCCACTTc                                                     >  1:241606/1‑93 (MQ=255)

                                     ccTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCa                                                    >  2:4542326/1‑93 (MQ=255)

                                     ccTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCa                                                    >  1:644692/1‑93 (MQ=255)

                                       tCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAgg                                                  >  2:119694/1‑93 (MQ=255)

                                       tCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAgg                                                  <  2:6063915/93‑1 (MQ=255)

                                       tCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAgg                                                  >  2:4423618/1‑93 (MQ=255)

                                       tCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAgg                                                  >  1:2018596/1‑93 (MQ=255)

                                       tCATAATAGTTGGACCCGTTGTGGCACCGCACAGGCGTAATACTTAGCAGGGGATAACAATGAAAATTTTCCAAAGGTACAACCCAATTCAgg                                                  <  2:1685328/93‑1 (MQ=255)

                                        cATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGt                                                 >  1:6322476/1‑93 (MQ=255)

                                            ataGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCg                                             >  2:5272406/1‑93 (MQ=255)

                                            ataGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGGACAACCCACTTCAGGTGGCg                                             >  1:4304170/1‑93 (MQ=255)

                                             taGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGa                                            <  2:5211585/93‑1 (MQ=255)

                                              aGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGaa                                           >  2:1739257/1‑93 (MQ=255)

                                              aGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGaa                                           <  1:1082788/93‑1 (MQ=255)

                                               gTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAg                                          >  1:4899769/1‑93 (MQ=255)

                                                ttGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGt                                         <  1:1119383/93‑1 (MQ=255)

                                                    gCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGt                                     >  1:3684843/1‑93 (MQ=255)

                                                        aTTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAg                                 <  1:2995743/93‑1 (MQ=255)

                                                        aTTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAg                                 >  2:4814834/1‑93 (MQ=255)

                                                            tgGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATcc                             <  2:2624998/93‑1 (MQ=255)

                                                             ggCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCt                            <  1:4375787/93‑1 (MQ=255)

                                                             ggCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCt                            <  1:5578880/93‑1 (MQ=255)

                                                             ggCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCt                            <  2:2220408/93‑1 (MQ=255)

                                                              gCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTg                           <  2:4909312/93‑1 (MQ=255)

                                                              gCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTg                           >  2:3667378/1‑93 (MQ=255)

                                                                aCCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGtt                         >  2:3117766/1‑93 (MQ=255)

                                                                aCCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGtt                         >  1:2782594/1‑93 (MQ=255)

                                                                 ccGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTc                        <  1:3415076/93‑1 (MQ=255)

                                                                   gCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCg                      <  1:6117970/93‑1 (MQ=255)

                                                                   gCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCg                      >  1:5804580/1‑93 (MQ=255)

                                                                   gCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCg                      >  2:1398818/1‑93 (MQ=255)

                                                                    cacaGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGt                     >  2:4263079/1‑93 (MQ=255)

                                                                    cacaGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGt                     >  2:2729994/1‑93 (MQ=255)

                                                                     acaGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTg                    >  2:1076709/1‑93 (MQ=255)

                                                                      caGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTgg                   >  2:2363099/1‑93 (MQ=255)

                                                                       aGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGa                  <  1:5042771/93‑1 (MQ=255)

                                                                        ggCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAc                 <  2:6149875/93‑1 (MQ=255)

                                                                        ggCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAc                 >  1:2253045/1‑93 (MQ=255)

                                                                        ggCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAc                 <  2:5043952/93‑1 (MQ=255)

                                                                            tAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTTGACGGtt             >  2:4834553/1‑93 (MQ=255)

                                                                            tAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGtt             >  2:46790/1‑93 (MQ=255)

                                                                               tACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTtata          >  1:1656445/1‑93 (MQ=255)

                                                                                aCTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATAc         <  1:6164145/93‑1 (MQ=255)

                                                                                aCTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATAc         <  2:2668620/93‑1 (MQ=255)

                                                                                aCTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATAc         >  1:2574451/1‑93 (MQ=255)

                                                                                 cTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACa        <  2:1606327/93‑1 (MQ=255)

                                                                                 cTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACa        >  2:2906358/1‑93 (MQ=255)

                                                                                   cagcagGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATc      <  2:1941122/93‑1 (MQ=255)

                                                                                    agcagGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCa     >  1:3489240/1‑93 (MQ=255)

                                                                                     gcagGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCaa    >  2:3525330/1‑93 (MQ=255)

                                                                                     gcagGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCaa    >  1:4188783/1‑93 (MQ=255)

                                                                                       agGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAgg  <  2:3783038/93‑1 (MQ=255)

CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGG  >  NC_000913/4437066‑4437245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGT  >  NC_000913/4437063‑4437249

TGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGG                                                                                               <  SRR4302537.3167839/94‑1 (MQ=60)

TGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGG                                                                                               >  SRR4302537.5443932/1‑94 (MQ=60)

TGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGAGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGG                                                                                               >  SRR4302537.3315081/1‑94 (MQ=60)

 GCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGG                                                                                              <  SRR4302537.106729/94‑1 (MQ=60)

  CCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGA                                                                                             >  SRR4302537.5119606/1‑94 (MQ=60)

   CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAT                                                                                            <  SRR4302537.6053427/94‑1 (MQ=60)

   CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAT                                                                                            >  SRR4302537.3302599/1‑94 (MQ=60)

   CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAT                                                                                            <  SRR4302537.3394995/94‑1 (MQ=60)

   CCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGAT                                                                                            >  SRR4302537.3679907/1‑94 (MQ=60)

    CGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATA                                                                                           <  SRR4302537.3604844/94‑1 (MQ=60)

    CGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATA                                                                                           <  SRR4302537.1855410/94‑1 (MQ=60)

    CGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGAACCGCACAGGCGTAATACTCAGCAGGGGATA                                                                                           >  SRR4302537.1368657/1‑94 (MQ=60)

     GGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAA                                                                                          >  SRR4302537.6159992/1‑94 (MQ=60)

     GGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAA                                                                                          >  SRR4302537.5153476/1‑94 (MQ=60)

     GGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAA                                                                                          >  SRR4302537.5763960/1‑94 (MQ=60)

     GGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAA                                                                                          <  SRR4302537.6350289/94‑1 (MQ=60)

     GGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAA                                                                                          <  SRR4302537.1725225/94‑1 (MQ=60)

      GATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAAC                                                                                         <  SRR4302537.4110269/94‑1 (MQ=60)

       ATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGTCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACA                                                                                        <  SRR4302537.645200/94‑1 (MQ=60)

        TAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAA                                                                                       >  SRR4302537.4660136/1‑94 (MQ=60)

         AGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAAT                                                                                      >  SRR4302537.1498079/1‑94 (MQ=60)

         AGT‑TATAGTCACTAAGCATTAAAATTTGCGCCTTATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATG                                                                                     <  SRR4302537.346721/94‑1 (MQ=60)

          GTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATG                                                                                     >  SRR4302537.6151752/1‑94 (MQ=60)

          GTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATG                                                                                     >  SRR4302537.4769908/1‑94 (MQ=60)

           TCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGA                                                                                    >  SRR4302537.1715525/1‑94 (MQ=60)

           TCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGA                                                                                    >  SRR4302537.2582029/1‑94 (MQ=60)

            CTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAA                                                                                   >  SRR4302537.798565/1‑94 (MQ=60)

             TATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAA                                                                                  >  SRR4302537.1492461/1‑94 (MQ=60)

              ATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAA                                                                                 >  SRR4302537.5384612/1‑94 (MQ=60)

                AGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATT                                                                               >  SRR4302537.1626184/1‑94 (MQ=60)

                 GTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTT                                                                              >  SRR4302537.1237991/1‑94 (MQ=60)

                  TCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATACCAATGAAAATTTT                                                                             <  SRR4302537.1539303/94‑1 (MQ=60)

                    ACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCC                                                                           <  SRR4302537.2891995/94‑1 (MQ=60)

                    ACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGACCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCC                                                                           <  SRR4302537.3038982/94‑1 (MQ=60)

                     CTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCA                                                                          >  SRR4302537.6440501/1‑94 (MQ=60)

                     CTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCA                                                                          >  SRR4302537.6181940/1‑94 (MQ=60)

                     CTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCA                                                                          <  SRR4302537.6142046/94‑1 (MQ=60)

                      TAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAA                                                                         >  SRR4302537.1128168/1‑94 (MQ=60)

                      TAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAA                                                                         <  SRR4302537.2911910/94‑1 (MQ=60)

                      TAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAA                                                                         >  SRR4302537.5409841/1‑94 (MQ=60)

                      TAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAA                                                                         <  SRR4302537.2833259/94‑1 (MQ=60)

                      TAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAA                                                                         <  SRR4302537.1756020/94‑1 (MQ=60)

                       AAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCGAC                                                                        <  SRR4302537.3223001/94‑1 (MQ=60)

                       AAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAAC                                                                        <  SRR4302537.4254626/94‑1 (MQ=60)

                        AGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACG                                                                       >  SRR4302537.2841699/1‑94 (MQ=60)

                        AGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACG                                                                       >  SRR4302537.5468603/1‑94 (MQ=60)

                        AGCATTAAAATTTGCGCCTCATAACAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACG                                                                       >  SRR4302537.2261007/1‑94 (MQ=60)

                          CATTAAAATTTGCGCCTCATAATAGTTGGTCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGT                                                                     <  SRR4302537.2663793/94‑1 (MQ=60)

                          CATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGT                                                                     <  SRR4302537.5110529/94‑1 (MQ=60)

                          CATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGT                                                                     >  SRR4302537.4106242/1‑94 (MQ=60)

                           ATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTA                                                                    >  SRR4302537.771227/1‑94 (MQ=60)

                           ATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTA                                                                    >  SRR4302537.2782692/1‑94 (MQ=60)

                           ATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTA                                                                    >  SRR4302537.6267299/1‑94 (MQ=60)

                             TAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACA                                                                  >  SRR4302537.1507150/1‑94 (MQ=60)

                               AAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAAC                                                                >  SRR4302537.6059042/1‑94 (MQ=60)

                               AAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAAC                                                                <  SRR4302537.6443150/94‑1 (MQ=60)

                                AATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACC                                                               <  SRR4302537.5473781/94‑1 (MQ=60)

                                 ATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCC                                                              >  SRR4302537.331403/1‑94 (MQ=60)

                                 ATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCC                                                              >  SRR4302537.3401223/1‑94 (MQ=60)

                                 ATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCC                                                              >  SRR4302537.4959494/1‑94 (MQ=60)

                                 ATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCC                                                              >  SRR4302537.3675595/1‑94 (MQ=60)

                                  TTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCA                                                             <  SRR4302537.52918/94‑1 (MQ=60)

                                   TTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCAC                                                            >  SRR4302537.4746869/1‑94 (MQ=60)

                                    TGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACT                                                           <  SRR4302537.4503973/94‑1 (MQ=60)

                                     GCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTT                                                          <  SRR4302537.2831630/94‑1 (MQ=60)

                                      CGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAGTTTTCCAACGGTACAACCCACTTC                                                         >  SRR4302537.243318/1‑94 (MQ=60)

                                      CGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTC                                                         >  SRR4302537.2638714/1‑94 (MQ=60)

                                      CGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTC                                                         >  SRR4302537.1098824/1‑94 (MQ=60)

                                       GCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCA                                                        >  SRR4302537.649080/1‑94 (MQ=60)

                                       GCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCA                                                        >  SRR4302537.3784948/1‑94 (MQ=60)

                                       GCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCA                                                        >  SRR4302537.3506403/1‑94 (MQ=60)

                                        CCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAG                                                       >  SRR4302537.3689864/1‑94 (MQ=60)

                                        CCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAG                                                       <  SRR4302537.4393251/94‑1 (MQ=60)

                                         CTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGG                                                      >  SRR4302537.2032673/1‑94 (MQ=60)

                                          TCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGT                                                     >  SRR4302537.3539659/1‑94 (MQ=60)

                                          TCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGT                                                     >  SRR4302537.6365733/1‑94 (MQ=60)

                                          TCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGT                                                     <  SRR4302537.2499983/94‑1 (MQ=60)

                                           CATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTG                                                    <  SRR4302537.2330164/94‑1 (MQ=60)

                                            ATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGG                                                   <  SRR4302537.187082/94‑1 (MQ=60)

                                            ATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGG                                                   >  SRR4302537.6228460/1‑94 (MQ=60)

                                            ATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGG                                                   >  SRR4302537.5032271/1‑94 (MQ=60)

                                             TAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGC                                                  >  SRR4302537.2632587/1‑94 (MQ=60)

                                              AATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCG                                                 >  SRR4302537.6314085/1‑94 (MQ=60)

                                              AATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCG                                                 >  SRR4302537.6393835/1‑94 (MQ=60)

                                              AATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGGACAACCCACTTCAGGTGGCG                                                 >  SRR4302537.4334082/1‑94 (MQ=60)

                                               ATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGA                                                >  SRR4302537.4682365/1‑94 (MQ=60)

                                                 AGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAG                                              >  SRR4302537.4809688/1‑94 (MQ=60)

                                                 AGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAG                                              >  SRR4302537.4933591/1‑94 (MQ=60)

                                                 AGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAG                                              <  SRR4302537.1090262/94‑1 (MQ=60)

                                                   TTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTA                                            <  SRR4302537.1967145/94‑1 (MQ=60)

                                                   TTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTA                                            <  SRR4302537.1127135/94‑1 (MQ=60)

                                                    TGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTAC                                           >  SRR4302537.6204863/1‑94 (MQ=60)

                                                     GGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACG                                          <  SRR4302537.3745558/94‑1 (MQ=60)

                                                      GGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGT                                         >  SRR4302537.3710759/1‑94 (MQ=60)

                                                        CCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAA                                       >  SRR4302537.1303459/1‑94 (MQ=60)

                                                         CGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAA                                      >  SRR4302537.3404051/1‑94 (MQ=60)

                                                          GATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAG                                     <  SRR4302537.6366247/94‑1 (MQ=60)

                                                          GATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAG                                     >  SRR4302537.1231849/1‑94 (MQ=60)

                                                          GATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAG                                     >  SRR4302537.1508453/1‑94 (MQ=60)

                                                           ATTGTGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGA                                    <  SRR4302537.3016633/94‑1 (MQ=60)

                                                               TGGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCT                                <  SRR4302537.5187404/94‑1 (MQ=60)

                                                                GGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTG                               <  SRR4302537.5617010/94‑1 (MQ=60)

                                                                GGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTG                               <  SRR4302537.1691310/94‑1 (MQ=60)

                                                                GGCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTG                               <  SRR4302537.4406007/94‑1 (MQ=60)

                                                                 GCACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGT                              <  SRR4302537.1566106/94‑1 (MQ=60)

                                                                  CACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTT                             >  SRR4302537.2802047/1‑94 (MQ=60)

                                                                  CACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTT                             <  SRR4302537.3299660/94‑1 (MQ=60)

                                                                  CACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTT                             <  SRR4302537.3285255/94‑1 (MQ=60)

                                                                   ACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTC                            <  SRR4302537.2841327/94‑1 (MQ=60)

                                                                   ACCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTC                            <  SRR4302537.574581/94‑1 (MQ=60)

                                                                    CCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCC                           <  SRR4302537.3439258/94‑1 (MQ=60)

                                                                    CCGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCC                           <  SRR4302537.3602765/94‑1 (MQ=60)

                                                                     CGCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCG                          >  SRR4302537.5844382/1‑94 (MQ=60)

                                                                      GCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGT                         <  SRR4302537.2420368/94‑1 (MQ=60)

                                                                      GCACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGT                         <  SRR4302537.6160020/94‑1 (MQ=60)

                                                                       CACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTG                        <  SRR4302537.3634211/94‑1 (MQ=60)

                                                                        ACAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGG                       >  SRR4302537.5581598/1‑94 (MQ=60)

                                                                         CAGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGA                      >  SRR4302537.2735385/1‑94 (MQ=60)

                                                                          AGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAC                     >  SRR4302537.2268948/1‑94 (MQ=60)

                                                                          AGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAC                     >  SRR4302537.4465729/1‑94 (MQ=60)

                                                                          AGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAC                     <  SRR4302537.6117379/94‑1 (MQ=60)

                                                                          AGGCGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGAC                     <  SRR4302537.5077499/94‑1 (MQ=60)

                                                                             CGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGT                  <  SRR4302537.4202825/94‑1 (MQ=60)

                                                                             CGTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGT                  <  SRR4302537.4283125/94‑1 (MQ=60)

                                                                              GTAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGGACGTATAGATCCTGTTACGTGGACGGTT                 >  SRR4302537.666182/1‑94 (MQ=60)

                                                                               TAATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTA                <  SRR4302537.3100758/94‑1 (MQ=60)

                                                                                AATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTAT               <  SRR4302537.1245127/94‑1 (MQ=60)

                                                                                 ATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATA              >  SRR4302537.1697624/1‑94 (MQ=60)

                                                                                 ATACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATA              >  SRR4302537.1668053/1‑94 (MQ=60)

                                                                                  TACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATAC             <  SRR4302537.2963355/94‑1 (MQ=60)

                                                                                  TACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATAC             >  SRR4302537.2592474/1‑94 (MQ=60)

                                                                                   ACTCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACA            <  SRR4302537.6206573/94‑1 (MQ=60)

                                                                                     TCAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATC          <  SRR4302537.885477/94‑1 (MQ=60)

                                                                                      CAGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCA         >  SRR4302537.3513921/1‑94 (MQ=60)

                                                                                       AGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAA        >  SRR4302537.5852869/1‑94 (MQ=60)

                                                                                       AGCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAA        >  SRR4302537.4218037/1‑94 (MQ=60)

                                                                                        GCAGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAG       <  SRR4302537.4424196/94‑1 (MQ=60)

                                                                                          AGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGA     >  SRR4302537.3210195/1‑94 (MQ=60)

                                                                                          AGGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGA     >  SRR4302537.515729/1‑94 (MQ=60)

                                                                                           GGGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGAC    <  SRR4302537.6147161/94‑1 (MQ=60)

                                                                                            GGGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACG   <  SRR4302537.153711/94‑1 (MQ=60)

                                                                                             GGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGT  >  SRR4302537.4061883/1‑94 (MQ=60)

                                                                                             GGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGT  >  SRR4302537.791844/1‑94 (MQ=60)

TGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGT  >  NC_000913/4437063‑4437249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`