Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,543	+C	intergenic (+48/‑90)	ytfQ → / → ytfR	putative LACI‑type transcriptional regulator/YtfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,542	1	.	C	98.9%	563.4 / ‑0.7	189	intergenic (+47/‑91)	ytfQ/ytfR	putative LACI‑type transcriptional regulator/YtfR
Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (124/63); total (126/63)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-02

Only 100 of 208 total aligned reads displayed.

AAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CG‑‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448461‑4448623

aagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCgg                                                                          <  1:5927692/93‑1 (MQ=37)

 agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGc                                                                         >  1:3948877/1‑93 (MQ=37)

  gaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCg                                                                        >  1:1956416/1‑93 (MQ=37)

   aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGa                                                                       >  2:4547762/1‑93 (MQ=37)

   aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGa                                                                       >  1:601408/1‑93 (MQ=37)

   aaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGa                                                                       >  2:2813116/1‑93 (MQ=37)

   aaTAAGAGAAGAAAAAAAATGGGGGGTATTGGGGTTTGTTTTGCTTGATGCCGATTGGTGGGCGGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGa                                                                       <  2:1129752/93‑1 (MQ=12)

     ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATg                                                                     >  1:1123993/1‑93 (MQ=37)

     ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATg                                                                     >  1:4833749/1‑93 (MQ=37)

      tAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGCTgg                                                                    >  2:1055180/1‑93 (MQ=25)

      tAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATgg                                                                    >  1:2541668/1‑93 (MQ=37)

        gagaAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCAGCCGGCGATGGTg                                                                  <  1:1885010/93‑1 (MQ=37)

         agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGc                                                                 >  1:2138536/1‑93 (MQ=37)

         agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGc                                                                 >  2:5188080/1‑93 (MQ=37)

         agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGc                                                                 >  2:1561515/1‑93 (MQ=37)

          gaagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCa                                                                >  1:2131640/1‑93 (MQ=37)

          gaagaagaaAAATATGGGCTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCa                                                                >  2:4289368/1‑93 (MQ=25)

           aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCAc                                                               <  1:2037700/93‑1 (MQ=37)

            agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACt                                                              >  1:6286603/1‑93 (MQ=37)

            agaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACt                                                              >  1:2734034/1‑93 (MQ=37)

             gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTg                                                             >  1:4969426/1‑93 (MQ=37)

                gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAg                                                          >  1:2603922/1‑93 (MQ=37)

                 aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGc                                                         <  2:2212541/93‑1 (MQ=37)

                  aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGcc                                                        >  2:4784705/1‑93 (MQ=37)

                  aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGcc                                                        <  2:828969/93‑1 (MQ=37)

                  aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGcc                                                        >  2:6238680/1‑93 (MQ=37)

                   aaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCt                                                       >  2:2612567/1‑93 (MQ=37)

                   aaaTATGGGGGATTGAGGGTTGCTATGCCTGATGACGATTCGTAGGCCGGATAAGGAGCT‑‑CGC‑GCC‑GCATCCGGCGATGGAGCACTGAAGACt                                                       >  2:4973519/1‑93 (MQ=21)

                     atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                     <  1:4046870/93‑1 (MQ=37)

                     atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                     >  2:1350176/1‑93 (MQ=37)

                     atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa                                                     >  2:3135143/1‑93 (MQ=37)

                      tatGGGGTATTGAGTGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    >  1:5810358/1‑93 (MQ=25)

                      tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGAt                                                    <  2:6356506/93‑1 (MQ=37)

                        tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                  >  2:782849/1‑93 (MQ=25)

                          gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                >  2:3688960/1‑93 (MQ=25)

                          gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGa                                                <  1:4345965/93‑1 (MQ=25)

                           ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGAc                                               <  1:2108265/93‑1 (MQ=25)

                            gTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg                                              <  2:2697703/93‑1 (MQ=25)

                             tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGc                                             >  2:5694857/1‑93 (MQ=25)

                             tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGc                                             >  1:2392998/1‑93 (MQ=25)

                              aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt                                            >  2:389217/1‑93 (MQ=25)

                                tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                          <  1:3063873/93‑1 (MQ=25)

                                tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                          <  2:6431171/93‑1 (MQ=25)

                                 gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         >  1:5743711/1‑93 (MQ=25)

                                 gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         >  1:3532158/1‑93 (MQ=25)

                                 gAGGGTGGCTATGCCTGAGGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc                                         <  2:189012/93‑1 (MQ=21)

                                  ggggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAGGGCCCC‑‑CGCCC‑C‑CCACCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        <  2:5532166/92‑1 (MQ=255)

                                  ggggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        <  2:4654580/92‑1 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  2:2374276/1‑93 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  2:853710/1‑93 (MQ=25)

                                  aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc                                        >  1:4454124/1‑93 (MQ=25)

                                     gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg                                     >  2:1012391/1‑93 (MQ=25)

                                     gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg                                     >  2:2674362/1‑93 (MQ=25)

                                      ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    >  1:2999926/1‑93 (MQ=25)

                                      ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt                                    <  1:6452758/93‑1 (MQ=25)

                                       tGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTc                                   >  2:3987178/1‑93 (MQ=25)

                                        gCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt                                  >  1:1307471/1‑93 (MQ=25)

                                         cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCtt                                 <  2:5001408/93‑1 (MQ=25)

                                          tATGCCTGATGCGGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                <  2:2487764/93‑1 (MQ=25)

                                          tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa                                >  2:5116920/1‑93 (MQ=25)

                                          tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATACGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACGGCGTCGTa                                >  2:217829/1‑93 (MQ=21)

                                           aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt                               >  1:917391/1‑93 (MQ=21)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              <  1:5956267/93‑1 (MQ=25)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              >  2:1199844/1‑93 (MQ=21)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              >  2:3923286/1‑93 (MQ=21)

                                            tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              <  2:4228549/93‑1 (MQ=25)

                                            agCCGGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc                              <  1:5196629/92‑1 (MQ=21)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCACG‑‑C‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             >  2:3341433/1‑93 (MQ=18)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             >  1:5378809/1‑93 (MQ=21)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             >  2:2661534/1‑93 (MQ=21)

                                             gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa                             <  1:1288586/93‑1 (MQ=21)

                                              ccTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                            >  2:718301/1‑93 (MQ=21)

                                              ccTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                            >  2:6169391/1‑93 (MQ=21)

                                               cTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg                           >  1:3371453/1‑93 (MQ=21)

                                                tGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc                          >  2:658659/1‑93 (MQ=21)

                                                 gATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                         >  1:3491773/1‑93 (MQ=21)

                                                 gATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAACGCGTCTTATCATGcc                         >  1:4150368/1‑93 (MQ=18)

                                                     ccGATTCGTAGTCCGGATAAGGCGCT‑‑CGC‑G‑CAGCATACGACGATCGTGAACTGAAGACTGATGAGGCGCGTAGCGCGTCATATGGTGCCTAcc                     >  2:4011969/1‑92 (MQ=12)

                                                     ccGATTCGTAGGCCGGATAGGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt                     <  2:3550745/93‑1 (MQ=21)

                                                     ccGATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt                     <  2:5722613/93‑1 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   <  1:3728083/93‑1 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   >  2:1742047/1‑93 (MQ=21)

                                                       gATTCGTAGGCCGGATAAGGCGCG‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                   >  2:5200831/1‑93 (MQ=18)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  2:5878924/1‑93 (MQ=21)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  2:591736/1‑93 (MQ=21)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  2:2149648/1‑93 (MQ=21)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTggg                  >  2:5310222/1‑93 (MQ=21)

                                                        aTTCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCGTACCGCGTCTTATCATGCCTACTggg                  >  1:5592522/1‑93 (MQ=18)

                                                         ttCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                 <  1:2896692/93‑1 (MQ=21)

                                                         ttCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGa                 >  2:4335709/1‑93 (MQ=21)

                                                         ttCGTAGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTCAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgggg                 >  1:4585171/1‑92 (MQ=18)

                                                              aGGCCGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACg            >  2:5147350/1‑93 (MQ=21)

                                                                 ccGGATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCtt         <  2:4295382/93‑1 (MQ=21)

                                                                  cagATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCttt        <  1:1729252/91‑1 (MQ=21)

                                                                    gATAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTac      <  2:6127478/93‑1 (MQ=25)

                                                                      tgaGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    <  1:81996/91‑1 (MQ=25)

                                                                      tAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    >  2:4837681/1‑93 (MQ=25)

                                                                      tAAGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTacac    >  1:152636/1‑93 (MQ=25)

                                                                        aGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg  >  2:4893109/1‑93 (MQ=25)

                                                                        aGGCGCT‑‑CGC‑GCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCg  >  1:1196442/1‑93 (MQ=25)

AAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCT‑‑CG‑‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG  >  NC_000913/4448461‑4448623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A