Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,7371.G99.6% 860.5 / ‑4.7 248C369G (TGC→GGC) ytfRYtfR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (119/128);  minor base A (1/0);  total (120/128)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 273 total aligned reads displayed.

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824
                                                                                             |                                                                                       
gCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          >  1:4758592/1‑93 (MQ=255)
gCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          <  2:3131124/93‑1 (MQ=255)
  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       >  1:6464652/1‑92 (MQ=255)
  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       >  1:5745459/1‑92 (MQ=255)
    cTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggct                                                                                     <  1:3023940/93‑4 (MQ=255)
     tGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggcta                                                                                    <  2:225633/93‑5 (MQ=255)
       cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACg                                                                                   <  2:62603/93‑1 (MQ=255)
       cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACg                                                                                   >  1:5183376/1‑93 (MQ=255)
       cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACg                                                                                   >  1:2022119/1‑93 (MQ=255)
        ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGt                                                                                  >  2:2226923/1‑93 (MQ=255)
        ccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGt                                                                                  >  2:4932836/1‑93 (MQ=255)
          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                >  2:1893179/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               >  2:5008208/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               >  1:317087/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               >  2:537286/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               >  2:5484655/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               >  1:1930386/1‑93 (MQ=255)
            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              <  1:3376798/93‑1 (MQ=255)
             aGACGGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAt                                                                             <  2:1009804/93‑1 (MQ=255)
             aGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAt                                                                             >  1:5057179/1‑93 (MQ=255)
             aGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATAATCCTCGCTCTCCAGAGCCAGCGCGGCTGGCTACGTCCCAt                                                                             >  1:2083740/1‑93 (MQ=255)
             aGACAGGAAAACCGATGCCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAt                                                                             <  1:868968/93‑1 (MQ=255)
              gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAtt                                                                            <  1:3545758/93‑1 (MQ=255)
               aCAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAttt                                                                           <  1:5879880/93‑1 (MQ=255)
                 aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTcc                                                                         <  1:2735803/93‑1 (MQ=255)
                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        >  2:4320081/1‑93 (MQ=255)
                  ggAAAACCGATGGCAGCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        <  1:2954195/93‑1 (MQ=255)
                     aaaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCa                                                                     <  1:3885406/93‑1 (MQ=255)
                       aCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGGTACGTCCCATTTCCCGCaaa                                                                   >  2:3959040/1‑93 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  <  1:4018601/93‑1 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  >  2:5656147/1‑93 (MQ=255)
                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTGCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  1:2257281/93‑1 (MQ=255)
                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  2:6352546/93‑1 (MQ=255)
                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 >  1:2641053/1‑93 (MQ=255)
                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               <  2:5972047/93‑1 (MQ=255)
                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCACGCCCAGCGCGTCTGGCTACGTCGCATTTCCCGCAAAGAAc                                                               >  1:918986/1‑93 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              >  1:383665/1‑93 (MQ=255)
                             ggCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAg                                                             <  1:5948581/93‑1 (MQ=255)
                              gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCACATTTCCCGCAAAGAACAGc                                                            <  1:612189/93‑1 (MQ=255)
                               catcatCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                           >  2:557694/1‑93 (MQ=255)
                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          >  1:30945/1‑93 (MQ=255)
                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  2:3770754/1‑93 (MQ=255)
                                  catcGCTGCCGCCTCGGTGCGGGAAAATCTCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAaga                                                        <  1:6219303/93‑1 (MQ=255)
                                   atcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                       >  2:3850329/1‑93 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      >  2:3620600/1‑93 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      <  1:4634201/93‑1 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     >  1:5522123/1‑93 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  2:6107003/93‑1 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  1:1494179/93‑1 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  1:813816/93‑1 (MQ=255)
                                      gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAtt                                                    >  2:1848404/1‑93 (MQ=255)
                                       cTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                   <  2:1162288/93‑1 (MQ=255)
                                       cTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                   >  2:1343197/1‑93 (MQ=255)
                                       cTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                   <  2:927453/93‑1 (MQ=255)
                                           cgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGa                                               <  1:5548664/93‑1 (MQ=255)
                                            cccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              <  2:3670805/92‑1 (MQ=255)
                                            cccTCGGTGCGGAAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              <  2:327877/92‑1 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  1:2403799/93‑1 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  2:2480078/93‑1 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  2:4749475/93‑1 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  2:2085153/93‑1 (MQ=255)
                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCCTTTCCCGCAAAGAACAGAAAGAGATTGCCGAACGc                                           >  1:2830428/1‑93 (MQ=255)
                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                           >  1:5297671/1‑93 (MQ=255)
                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                           >  2:1595417/1‑93 (MQ=255)
                                                cGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCt                                          <  2:4247091/93‑1 (MQ=255)
                                                cGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCt                                          <  2:1504/93‑1 (MQ=255)
                                                 ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCGAAGAACAGCAAGAGATTGCCGAACGCtt                                         >  1:4227840/1‑93 (MQ=255)
                                                  gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCttt                                        <  1:1150249/93‑1 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     >  1:5309865/1‑93 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     <  2:5125737/93‑1 (MQ=255)
                                                      gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                    >  1:1291053/1‑93 (MQ=255)
                                                       gtaaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCg                                   <  2:246490/91‑1 (MQ=255)
                                                        gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGc                                  >  2:1638336/1‑93 (MQ=255)
                                                         aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                 <  2:5626090/93‑1 (MQ=255)
                                                         aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                 <  1:2299854/93‑1 (MQ=255)
                                                          aaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCa                                >  1:3261806/1‑93 (MQ=255)
                                                          aaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCa                                >  1:1547748/1‑93 (MQ=255)
                                                          aaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCa                                <  1:6014785/93‑1 (MQ=255)
                                                            atatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGc                              <  1:3740215/93‑1 (MQ=255)
                                                             tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                             <  1:1442441/93‑1 (MQ=255)
                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCCGCtt                            >  2:2518595/1‑93 (MQ=255)
                                                                catcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                          <  1:1575420/93‑1 (MQ=255)
                                                                  tcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCa                        <  1:4954176/93‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  1:4179357/93‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  2:824246/93‑1 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGTTTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     <  1:6307361/93‑1 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     >  2:435445/1‑93 (MQ=255)
                                                                         tctcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcac                 >  1:6343043/1‑93 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac               >  2:4552876/1‑93 (MQ=255)
                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              <  1:4467689/93‑1 (MQ=255)
                                                                              aGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt            <  2:5269338/93‑1 (MQ=255)
                                                                               ggCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc           <  1:2601732/93‑1 (MQ=255)
                                                                               ggCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc           >  2:4867618/1‑93 (MQ=255)
                                                                                gtccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCa          >  1:1000967/3‑93 (MQ=255)
                                                                                  ccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAc        <  1:175486/93‑1 (MQ=255)
                                                                                    aGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg      <  2:5522123/93‑1 (MQ=255)
                                                                                    aGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg      <  2:583283/93‑1 (MQ=255)
                                                                                    aGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg      >  2:5872859/1‑93 (MQ=255)
                                                                                      cgcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaa    <  2:4176821/93‑1 (MQ=255)
                                                                                       gcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaac   >  2:2616182/1‑93 (MQ=255)
                                                                                             |                                                                                       
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  NC_000913/4449645‑4449831
                                                                                              |                                                                                             
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑G                                                                                               <  SRR4302537.5075061/94‑1 (MQ=60)
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑G                                                                                               >  SRR4302537.1372676/1‑94 (MQ=60)
 CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                              >  SRR4302537.5784805/1‑94 (MQ=60)
 CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                              >  SRR4302537.6508443/1‑94 (MQ=60)
  TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggc                                                                                             <  SRR4302537.1960360/94‑3 (MQ=60)
    CTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTA                                                                                            >  SRR4302537.6261553/1‑94 (MQ=60)
    CTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTA                                                                                            <  SRR4302537.3045020/94‑1 (MQ=60)
     TGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTAC                                                                                           <  SRR4302537.333125/94‑1 (MQ=60)
      GCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACG                                                                                          >  SRR4302537.5219039/1‑94 (MQ=60)
      GCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACG                                                                                          >  SRR4302537.5219038/1‑94 (MQ=60)
      GCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACG                                                                                          >  SRR4302537.2036228/1‑94 (MQ=60)
      GCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCAGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACG                                                                                          >  SRR4302537.4590098/1‑94 (MQ=60)
        CCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTC                                                                                        >  SRR4302537.3472665/1‑94 (MQ=60)
        CCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTC                                                                                        >  SRR4302537.4117392/1‑94 (MQ=60)
         CGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCC                                                                                       >  SRR4302537.1951484/1‑94 (MQ=60)
          GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                      >  SRR4302537.319285/1‑94 (MQ=60)
          GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                      >  SRR4302537.1943814/1‑94 (MQ=60)
            AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAT                                                                                    <  SRR4302537.2838796/94‑1 (MQ=60)
            AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAT                                                                                    >  SRR4302537.5091993/1‑94 (MQ=60)
            AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAT                                                                                    <  SRR4302537.3400730/94‑1 (MQ=60)
            AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATAATCCTCGCTCTCCAGAGCCAGCGCGGCTGGCTACGTCCCAT                                                                                    >  SRR4302537.2098423/1‑94 (MQ=60)
             AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                   >  SRR4302537.6368751/1‑94 (MQ=60)
             AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                   >  SRR4302537.5571486/1‑94 (MQ=60)
             AGACAGGAAAACCGATGCCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                   <  SRR4302537.874935/94‑1 (MQ=60)
              GACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTT                                                                                  <  SRR4302537.3570805/94‑1 (MQ=60)
               ACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTC                                                                                 <  SRR4302537.5920221/94‑1 (MQ=60)
                CAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATACTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCC                                                                                >  SRR4302537.893899/1‑94 (MQ=60)
                 AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                               >  SRR4302537.2631561/1‑94 (MQ=60)
                 AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                               >  SRR4302537.6299932/1‑94 (MQ=60)
                 AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                               <  SRR4302537.2754897/94‑1 (MQ=60)
                  GGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCG                                                                              >  SRR4302537.1533145/1‑94 (MQ=60)
                  GGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCG                                                                              <  SRR4302537.4208789/94‑1 (MQ=60)
                  GGAAAACCGATGGCAGCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCG                                                                              <  SRR4302537.2974925/94‑1 (MQ=60)
                     AAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAA                                                                           <  SRR4302537.3912586/94‑1 (MQ=60)
                        CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                        <  SRR4302537.4046735/94‑1 (MQ=60)
                        CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                        >  SRR4302537.2659530/1‑94 (MQ=60)
                        CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                        <  SRR4302537.6408307/94‑1 (MQ=60)
                        CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                        <  SRR4302537.1567763/94‑1 (MQ=60)
                         CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTGCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAA                                                                       <  SRR4302537.2273212/94‑1 (MQ=60)
                         CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAA                                                                       >  SRR4302537.1581389/1‑94 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAca                                                                      >  SRR4302537.1477519/1‑92 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                      <  SRR4302537.5784692/94‑1 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                      <  SRR4302537.835123/94‑1 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAA                                                                      >  SRR4302537.5012698/1‑94 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCACGCCCAGCGCGTCTGGCTACGTCGCATTTCCCGCAAAGAAC                                                                      >  SRR4302537.925331/1‑94 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     <  SRR4302537.5276123/94‑1 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     >  SRR4302537.3170990/1‑94 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     >  SRR4302537.386323/1‑94 (MQ=60)
                            TGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAG                                                                    >  SRR4302537.1712353/1‑94 (MQ=60)
                             GGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGC                                                                   <  SRR4302537.5989412/94‑1 (MQ=60)
                              GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCA                                                                  <  SRR4302537.591445/94‑1 (MQ=60)
                              GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCA                                                                  <  SRR4302537.6278369/94‑1 (MQ=60)
                              GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCA                                                                  <  SRR4302537.3823129/94‑1 (MQ=60)
                              GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCACATTTCCCGCAAAGAACAGCA                                                                  <  SRR4302537.616368/94‑1 (MQ=60)
                               CATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAA                                                                 >  SRR4302537.31193/1‑94 (MQ=60)
                                ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCACAGAACAGCAAG                                                                >  SRR4302537.211145/1‑94 (MQ=60)
                                ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                <  SRR4302537.5991640/94‑1 (MQ=60)
                                ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                <  SRR4302537.1313621/94‑1 (MQ=60)
                                  CATCGCTGCCGCCTCGGTGCGGGAAAATCTCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                              <  SRR4302537.6262122/94‑1 (MQ=60)
                                  CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                              <  SRR4302537.2304327/94‑1 (MQ=60)
                                   ATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGA                                                             >  SRR4302537.2438259/1‑94 (MQ=60)
                                    TCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAT                                                            <  SRR4302537.4666254/94‑1 (MQ=60)
                                    TCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAT                                                            >  SRR4302537.5559822/1‑94 (MQ=60)
                                     CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                           >  SRR4302537.2749433/1‑94 (MQ=60)
                                     CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                           <  SRR4302537.3553203/94‑1 (MQ=60)
                                     CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                           >  SRR4302537.208957/1‑94 (MQ=60)
                                     CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                           <  SRR4302537.1504585/94‑1 (MQ=60)
                                     CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                           <  SRR4302537.819393/94‑1 (MQ=60)
                                      GCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                          >  SRR4302537.4298802/1‑94 (MQ=60)
                                      GCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                          >  SRR4302537.5492476/1‑94 (MQ=60)
                                       CTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGC                                                         >  SRR4302537.3039256/1‑94 (MQ=60)
                                       CTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGC                                                         <  SRR4302537.98589/94‑1 (MQ=60)
                                          CCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGA                                                      <  SRR4302537.5346091/94‑1 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCTAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     >  SRR4302537.39961/1‑94 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     <  SRR4302537.5586552/94‑1 (MQ=60)
                                            GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                    <  SRR4302537.1022384/94‑1 (MQ=60)
                                            GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                    <  SRR4302537.680260/94‑1 (MQ=60)
                                             CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACG                                                   <  SRR4302537.215297/94‑1 (MQ=60)
                                             CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACG                                                   <  SRR4302537.2420722/94‑1 (MQ=60)
                                              CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCCTTTCCCGCAAAGAACAGAAAGAGATTGCCGAACGC                                                  >  SRR4302537.2850205/1‑94 (MQ=60)
                                              CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                  <  SRR4302537.3117596/94‑1 (MQ=60)
                                              CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                  >  SRR4302537.5334062/1‑94 (MQ=60)
                                                CGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCGAAGAACAGCAAGAGATTGCCGAACGCTT                                                >  SRR4302537.4257276/1‑94 (MQ=60)
                                                CGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTT                                                <  SRR4302537.5850334/94‑1 (MQ=60)
                                                 GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                               <  SRR4302537.5954817/94‑1 (MQ=60)
                                                  GTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTA                                              <  SRR4302537.1158245/94‑1 (MQ=60)
                                                  GTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTA                                              <  SRR4302537.2095266/94‑1 (MQ=60)
                                                    GCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATC                                            >  SRR4302537.5346345/1‑94 (MQ=60)
                                                     CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                           >  SRR4302537.1300031/1‑94 (MQ=60)
                                                     CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                           >  SRR4302537.6354006/1‑94 (MQ=60)
                                                      GGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCG                                          >  SRR4302537.1286405/1‑94 (MQ=60)
                                                      GGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCG                                          <  SRR4302537.524980/94‑1 (MQ=60)
                                                       GGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGC                                         >  SRR4302537.3096284/1‑94 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       >  SRR4302537.3284810/1‑94 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       >  SRR4302537.1512750/1‑94 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       <  SRR4302537.2316087/94‑1 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       <  SRR4302537.1224239/94‑1 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       >  SRR4302537.1558550/1‑94 (MQ=60)
                                                          AAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAG                                      <  SRR4302537.6056107/94‑1 (MQ=60)
                                                           AATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGC                                     >  SRR4302537.1720219/1‑94 (MQ=60)
                                                            ATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                    <  SRR4302537.5505634/94‑1 (MQ=60)
                                                            ATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                    <  SRR4302537.3766484/94‑1 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGACCAGCAAGAGAATGCCGAACGACTTATCCGCCAGCTT                                   >  SRR4302537.2628454/1‑94 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   <  SRR4302537.1452491/94‑1 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   >  SRR4302537.4514676/1‑94 (MQ=60)
                                                              ATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTG                                  <  SRR4302537.5577918/94‑1 (MQ=60)
                                                               TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                 <  SRR4302537.622196/94‑1 (MQ=60)
                                                               TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                 <  SRR4302537.274993/94‑1 (MQ=60)
                                                               TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                 <  SRR4302537.5927028/94‑1 (MQ=60)
                                                                CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                <  SRR4302537.1586406/94‑1 (MQ=60)
                                                                 ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                               >  SRR4302537.372981/1‑94 (MQ=60)
                                                                 ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                               >  SRR4302537.4712384/1‑94 (MQ=60)
                                                                  TCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAT                              <  SRR4302537.4988338/94‑1 (MQ=60)
                                                                   CCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATT                             <  SRR4302537.2470360/94‑1 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302537.6084025/94‑1 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302537.3046174/94‑1 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302537.4208529/94‑1 (MQ=60)
                                                                     TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGTTTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302537.6350552/94‑1 (MQ=60)
                                                                      CGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                          >  SRR4302537.2915178/1‑94 (MQ=60)
                                                                      CGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                          >  SRR4302537.3869388/1‑94 (MQ=60)
                                                                       GCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCA                         <  SRR4302537.804762/94‑1 (MQ=60)
                                                                        CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                        >  SRR4302537.6386377/1‑94 (MQ=60)
                                                                        CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                        <  SRR4302537.979553/94‑1 (MQ=60)
                                                                            CCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                    <  SRR4302537.4498586/94‑1 (MQ=60)
                                                                            CCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                    <  SRR4302537.6037259/94‑1 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   >  SRR4302537.2840169/1‑94 (MQ=60)
                                                                              AGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTC                  >  SRR4302537.4679920/1‑94 (MQ=60)
                                                                              AGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTC                  >  SRR4302537.838097/1‑94 (MQ=60)
                                                                               GGTCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 >  SRR4302537.1007875/1‑94 (MQ=60)
                                                                               GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 <  SRR4302537.237514/94‑1 (MQ=60)
                                                                               GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 <  SRR4302537.2619922/94‑1 (MQ=60)
                                                                                 CCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAC               >  SRR4302537.3572619/1‑94 (MQ=60)
                                                                                 CCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAC               >  SRR4302537.3268733/1‑94 (MQ=60)
                                                                                 CCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAC               >  SRR4302537.2926839/1‑94 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              >  SRR4302537.3879607/1‑94 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              <  SRR4302537.4367421/94‑1 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              <  SRR4302537.176728/94‑1 (MQ=60)
                                                                                   CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG             <  SRR4302537.1818395/94‑1 (MQ=60)
                                                                                   CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG             <  SRR4302537.3939733/94‑1 (MQ=60)
                                                                                   CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCAGCCAGCTTGGCATTCGCACACCTTCAACTG             >  SRR4302537.2378469/1‑94 (MQ=60)
                                                                                    AGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGA            >  SRR4302537.4794453/1‑94 (MQ=60)
                                                                                    AGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGA            >  SRR4302537.4057088/1‑94 (MQ=60)
                                                                                     GCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAA           >  SRR4302537.3246724/1‑94 (MQ=60)
                                                                                       GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA         <  SRR4302537.517338/94‑1 (MQ=60)
                                                                                         GGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAC       <  SRR4302537.1988837/94‑1 (MQ=60)
                                                                                          GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC      >  SRR4302537.4657157/1‑94 (MQ=60)
                                                                                          GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC      <  SRR4302537.1071799/94‑1 (MQ=60)
                                                                                            tgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGA    >  SRR4302537.4883881/3‑94 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  <  SRR4302537.2589479/94‑1 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  SRR4302537.3791512/1‑94 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  <  SRR4302537.3942725/94‑1 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  SRR4302537.6045884/1‑94 (MQ=60)
                                                                                              |                                                                                             
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  NC_000913/4449645‑4449831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑