Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,550,738 A→G E90G (GAG→GGG)  uxuB → D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,550,7380AG96.9% 841.2 / 10.5 257E90G (GAG→GGG) uxuBD‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (4/4);  new base G (148/101);  total (152/105)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 263 total aligned reads displayed.

TGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAA  >  NC_000913/4550648‑4550827
                                                                                          |                                                                                         
tGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGc                                                                                         <  2:2560973/93‑1 (MQ=255)
tGAGGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGc                                                                                         <  2:1107469/93‑1 (MQ=255)
      cAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGaaaa                                                                                   <  1:6112344/93‑1 (MQ=255)
       aGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAAt                                                                                  <  1:2257593/93‑1 (MQ=255)
        ggCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAAtt                                                                                 >  1:4806873/1‑93 (MQ=255)
          cAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTAt                                                                               <  2:1690259/93‑1 (MQ=255)
            aCGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCg                                                                             <  1:4642019/93‑1 (MQ=255)
            aCGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCg                                                                             <  2:2338019/93‑1 (MQ=255)
              gACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGt                                                                           <  1:1112013/93‑1 (MQ=255)
              gACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGt                                                                           <  1:448175/93‑1 (MQ=255)
              gACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAACTATCGGt                                                                           >  1:5484085/1‑93 (MQ=255)
                ccGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTc                                                                         <  2:868465/93‑1 (MQ=255)
                ccGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTc                                                                         >  1:2391392/1‑93 (MQ=255)
                ccGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTc                                                                         <  2:4709554/93‑1 (MQ=255)
                 cgcgTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCg                                                                        <  1:2663332/93‑1 (MQ=255)
                  gcgTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGa                                                                       >  1:3570180/1‑93 (MQ=255)
                    gTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATg                                                                     <  1:1240473/93‑1 (MQ=255)
                    gTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATg                                                                     >  1:2654539/1‑93 (MQ=255)
                     tGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGa                                                                    >  1:1087265/1‑93 (MQ=255)
                      gCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGaa                                                                   <  2:5319893/93‑1 (MQ=255)
                      gCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGaa                                                                   >  2:1487189/1‑93 (MQ=255)
                        tGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCCGAGAGCACCGGGCTGAAAATTATCGGTTCGATGaaaa                                                                 >  1:2952939/1‑92 (MQ=255)
                        tGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaag                                                                 >  1:757914/1‑93 (MQ=255)
                        tGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaag                                                                 <  1:4249912/93‑1 (MQ=255)
                          aTCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaagaa                                                               <  1:1659072/93‑1 (MQ=255)
                          aTCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaagaa                                                               >  1:4435714/1‑93 (MQ=255)
                           tCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaagaag                                                              >  1:3419913/1‑93 (MQ=255)
                           tCGAAAACATGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAaagaag                                                              <  2:5467660/93‑1 (MQ=255)
                             gAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAgcg                                                            >  1:1206760/1‑93 (MQ=255)
                             gAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAgcg                                                            <  1:954519/93‑1 (MQ=255)
                              aaaaCCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAgcgc                                                           <  2:2201857/93‑1 (MQ=255)
                              aaaaCCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAgcgc                                                           >  1:2160883/1‑93 (MQ=255)
                                aaCCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTg                                                         >  1:5291498/1‑93 (MQ=255)
                                  ccTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCa                                                       <  1:4632948/93‑1 (MQ=255)
                                  ccTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGAGCAGAGAGCACCGGGCTGCAAATTATCGGTTCGATGAAAGAAGCGCTGCa                                                       >  1:1478706/1‑93 (MQ=255)
                                   cTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAt                                                      <  2:4597412/93‑1 (MQ=255)
                                   cTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAt                                                      <  1:6174192/93‑1 (MQ=255)
                                   cTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAt                                                      <  1:4101778/93‑1 (MQ=255)
                                   cTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAt                                                      >  2:2965583/1‑93 (MQ=255)
                                    tGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATc                                                     >  2:4282222/1‑93 (MQ=255)
                                     gAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATcc                                                    <  2:5034025/93‑1 (MQ=255)
                                     gAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATcc                                                    >  2:764666/1‑93 (MQ=255)
                                     gAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATcc                                                    >  2:6165477/1‑93 (MQ=255)
                                     gAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATAAAAGAAGCGCTGCATcg                                                    >  1:4013878/1‑92 (MQ=255)
                                       aaaaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCgg                                                  >  1:1048826/1‑93 (MQ=255)
                                        aaaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGa                                                 <  1:1967091/93‑1 (MQ=255)
                                          aaCAGCACCTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGaaa                                               <  2:2825592/93‑1 (MQ=255)
                                          aaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGaaa                                               <  1:5757894/93‑1 (MQ=255)
                                           aCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAt                                              <  1:1333027/93‑1 (MQ=255)
                                           aCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAt                                              <  2:4229741/93‑1 (MQ=255)
                                            cAGCAACTGCTGTACAACGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATc                                             >  1:2305004/1‑93 (MQ=255)
                                              gCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGa                                           >  2:3629418/1‑93 (MQ=255)
                                               cAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGAt                                          <  2:4522641/93‑1 (MQ=255)
                                                aaCTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATg                                         <  2:5686770/93‑1 (MQ=255)
                                                aaCTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATg                                         >  2:4001914/1‑93 (MQ=255)
                                                 aCTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATgg                                        <  2:4353006/93‑1 (MQ=255)
                                                 aCTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATgg                                        >  2:3908872/1‑93 (MQ=255)
                                                  ctgctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGc                                       <  2:2391392/93‑1 (MQ=255)
                                                   tgctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCt                                      <  2:3518770/93‑1 (MQ=255)
                                                    gctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTg                                     >  1:5006270/1‑93 (MQ=255)
                                                    gctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTg                                     >  2:2709248/1‑93 (MQ=255)
                                                     ctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGc                                    >  2:5085863/1‑93 (MQ=255)
                                                     ctgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGc                                    >  2:3208188/1‑93 (MQ=255)
                                                      tgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCg                                   >  1:1595553/1‑93 (MQ=255)
                                                      tgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCg                                   >  1:3363875/1‑93 (MQ=255)
                                                      tgTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCg                                   >  2:1643918/1‑93 (MQ=255)
                                                       gTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGa                                  >  1:3602855/1‑93 (MQ=255)
                                                        tACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGaa                                 <  2:5946495/93‑1 (MQ=255)
                                                         acacCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATCAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAg                                >  2:1393526/1‑93 (MQ=255)
                                                          cacCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAgg                               >  2:6334159/1‑93 (MQ=255)
                                                            ccGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGtt                             >  1:5363877/1‑92 (MQ=255)
                                                            ccGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTa                             >  1:6260939/1‑93 (MQ=255)
                                                             cGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTAt                            >  2:5784610/1‑93 (MQ=255)
                                                             cGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTGCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTAt                            <  1:696143/93‑1 (MQ=255)
                                                              gTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTAtt                           <  2:2064048/93‑1 (MQ=255)
                                                              gTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTAtt                           <  2:3749329/93‑1 (MQ=255)
                                                               tAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATtc                          <  1:1833217/93‑1 (MQ=255)
                                                                 gCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATtctc                        <  2:4836556/93‑1 (MQ=255)
                                                                 gCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATtctc                        >  1:2808402/1‑93 (MQ=255)
                                                                  cGGAAAAAGGAGCAGAGAGCACCGGGCTGAAAATTATCGGATCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTCCCAAGGTATTCTCa                       >  1:2220484/1‑93 (MQ=255)
                                                                    gAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAc                     <  2:1033255/93‑1 (MQ=255)
                                                                      aaaaGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAcgc                   <  1:4303828/93‑1 (MQ=255)
                                                                      aaaaGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAcgc                   >  1:2976328/1‑93 (MQ=255)
                                                                      aaaaGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCAATGGCTGCGAAGGTATTCTCAAcgc                   <  1:2042810/93‑1 (MQ=255)
                                                                        aaGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGa                 <  1:3952341/93‑1 (MQ=255)
                                                                         aGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGAt                >  2:4464972/1‑93 (MQ=255)
                                                                         aGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGAt                <  1:4398111/93‑1 (MQ=255)
                                                                         aGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGAt                <  1:3286130/93‑1 (MQ=255)
                                                                          ggCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATg               >  2:3832662/1‑93 (MQ=255)
                                                                          ggCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATg               >  1:5912523/1‑93 (MQ=255)
                                                                          ggCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATg               >  1:3492089/1‑93 (MQ=255)
                                                                           gcgcAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATgg              >  2:3078546/1‑93 (MQ=255)
                                                                             gcAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGgcg            >  1:503105/1‑93 (MQ=255)
                                                                             gcAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGgcg            >  2:6229148/1‑93 (MQ=255)
                                                                               agagagCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGcgcg          >  2:2805289/1‑93 (MQ=255)
                                                                                gagagCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGt         >  2:189578/1‑93 (MQ=255)
                                                                                 agagCACCGGGCTGAAAATTATCGGTTCGATGAAAGCAGCGCTGCATACGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTc        >  1:1007733/1‑93 (MQ=255)
                                                                                 agagCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTc        >  2:5526606/1‑93 (MQ=255)
                                                                                 agagCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTc        <  1:565080/93‑1 (MQ=255)
                                                                                  gagCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTcc       <  2:68897/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAA  >  NC_000913/4550648‑4550827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGC  >  NC_000913/4550646‑4550831
                                                                                            |                                                                                             
CCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGG                                                                                              <  SRR4302537.2981739/94‑1 (MQ=60)
 CTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGACAAAGGCGCAGAGAGCACCGGGC                                                                                             >  SRR4302537.2319627/1‑94 (MQ=60)
 CTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGC                                                                                             <  SRR4302537.4196430/94‑1 (MQ=60)
  TGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCT                                                                                            <  SRR4302537.3166943/94‑1 (MQ=60)
  TGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCT                                                                                            >  SRR4302537.6182269/1‑94 (MQ=60)
   GATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTG                                                                                           <  SRR4302537.2099159/94‑1 (MQ=60)
   GATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTG                                                                                           <  SRR4302537.6255977/94‑1 (MQ=60)
       CCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAA                                                                                       >  SRR4302537.2673506/1‑94 (MQ=60)
        CAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGCGAGCACCGGGCTGAAAAT                                                                                      >  SRR4302537.5620632/1‑94 (MQ=60)
        CAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAAT                                                                                      <  SRR4302537.6154349/94‑1 (MQ=60)
        CAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAAT                                                                                      <  SRR4302537.778056/94‑1 (MQ=60)
        CAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGCCTGAAAAT                                                                                      >  SRR4302537.1936573/1‑94 (MQ=60)
         AGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATT                                                                                     >  SRR4302537.4840026/1‑94 (MQ=60)
         AGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATT                                                                                     <  SRR4302537.2273525/94‑1 (MQ=60)
         AGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATT                                                                                     >  SRR4302537.4443386/1‑94 (MQ=60)
         AGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATT                                                                                     >  SRR4302537.3561769/1‑94 (MQ=60)
            CAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATC                                                                                  <  SRR4302537.4395110/94‑1 (MQ=60)
             AACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCG                                                                                 >  SRR4302537.4270593/1‑94 (MQ=60)
             AACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCG                                                                                 >  SRR4302537.43030/1‑94 (MQ=60)
              ACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGG                                                                                <  SRR4302537.4674120/94‑1 (MQ=60)
               CGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAACTATCGGT                                                                               >  SRR4302537.5521521/1‑94 (MQ=60)
                GACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTT                                                                              <  SRR4302537.348306/94‑1 (MQ=60)
                GACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTT                                                                              <  SRR4302537.451283/94‑1 (MQ=60)
                GACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTT                                                                              >  SRR4302537.6044852/1‑94 (MQ=60)
                GACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTT                                                                              <  SRR4302537.1119691/94‑1 (MQ=60)
                GACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTT                                                                              <  SRR4302537.108289/94‑1 (MQ=60)
                 ACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTC                                                                             >  SRR4302537.2408234/1‑94 (MQ=60)
                  CCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCG                                                                            >  SRR4302537.964772/1‑94 (MQ=60)
                   CGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGA                                                                           <  SRR4302537.3954687/94‑1 (MQ=60)
                   CGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGA                                                                           <  SRR4302537.2681936/94‑1 (MQ=60)
                   CGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGA                                                                           >  SRR4302537.3595355/1‑94 (MQ=60)
                     CGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATG                                                                         >  SRR4302537.2673096/1‑94 (MQ=60)
                      GTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGA                                                                        <  SRR4302537.1249096/94‑1 (MQ=60)
                      GTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGA                                                                        >  SRR4302537.6254228/1‑94 (MQ=60)
                      GTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGA                                                                        >  SRR4302537.4656306/1‑94 (MQ=60)
                      GTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGA                                                                        >  SRR4302537.1094771/1‑94 (MQ=60)
                       TGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAA                                                                       >  SRR4302537.4378247/1‑94 (MQ=60)
                         CTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCCGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAA                                                                     >  SRR4302537.2973663/1‑94 (MQ=60)
                         CTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAG                                                                     >  SRR4302537.763088/1‑94 (MQ=60)
                         CTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAG                                                                     >  SRR4302537.1091228/1‑94 (MQ=60)
                          TGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGA                                                                    >  SRR4302537.5452439/1‑94 (MQ=60)
                          TGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGA                                                                    <  SRR4302537.4279444/94‑1 (MQ=60)
                          TGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGCAGCGGAAAAAGGCGCAGAGAGCACCGGACTGAAAATTATCGGTTCGATGAAAGA                                                                    >  SRR4302537.889222/1‑94 (MQ=60)
                           GATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAA                                                                   >  SRR4302537.4466342/1‑94 (MQ=60)
                           GATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAA                                                                   <  SRR4302537.5349287/94‑1 (MQ=60)
                           GATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAA                                                                   <  SRR4302537.466588/94‑1 (MQ=60)
                           GATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAA                                                                   <  SRR4302537.1309328/94‑1 (MQ=60)
                           GATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAA                                                                   <  SRR4302537.4500992/94‑1 (MQ=60)
                            ATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAG                                                                  >  SRR4302537.3444121/1‑94 (MQ=60)
                            ATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAG                                                                  <  SRR4302537.1670702/94‑1 (MQ=60)
                            ATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAG                                                                  >  SRR4302537.1106681/1‑94 (MQ=60)
                              CGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCG                                                                <  SRR4302537.3620723/94‑1 (MQ=60)
                              CGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCG                                                                >  SRR4302537.1215158/1‑94 (MQ=60)
                               GAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGC                                                               >  SRR4302537.2176117/1‑94 (MQ=60)
                               GAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGC                                                               >  SRR4302537.2911098/1‑94 (MQ=60)
                               GAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGC                                                               <  SRR4302537.961107/94‑1 (MQ=60)
                                 AAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTG                                                             >  SRR4302537.6513149/1‑94 (MQ=60)
                                 AAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTG                                                             >  SRR4302537.5327863/1‑94 (MQ=60)
                                  AACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGC                                                            <  SRR4302537.1708018/94‑1 (MQ=60)
                                   ACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCA                                                           >  SRR4302537.5917371/1‑94 (MQ=60)
                                   ACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGAGCAGAGAGCACCGGGCTGCAAATTATCGGTTCGATGAAAGAAGCGCTGCA                                                           >  SRR4302537.1488990/1‑94 (MQ=60)
                                    CCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAT                                                          >  SRR4302537.5763974/1‑94 (MQ=60)
                                    CCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAT                                                          <  SRR4302537.5957236/94‑1 (MQ=60)
                                    CCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCAT                                                          <  SRR4302537.4664997/94‑1 (MQ=60)
                                     CTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATC                                                         >  SRR4302537.2094237/1‑94 (MQ=60)
                                     CTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATC                                                         <  SRR4302537.1398195/94‑1 (MQ=60)
                                     CTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATC                                                         >  SRR4302537.3574765/1‑94 (MQ=60)
                                     CTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATC                                                         <  SRR4302537.4130418/94‑1 (MQ=60)
                                     CTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATC                                                         <  SRR4302537.6216714/94‑1 (MQ=60)
                                      gtaaagaacagcagctggtggtCACCGTAGCGGAAAAAGGCGGAGAGAGCACAGGGCTGAAAACTATCGGTTCGATGAAAGAAGCGCTGCATCC                                                        <  SRR4302537.3062721/72‑1 (MQ=60)
                                      TGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCC                                                        >  SRR4302537.707346/1‑94 (MQ=60)
                                      TGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATAAAAGAAGCGCTGCATCG                                                        >  SRR4302537.4041986/1‑94 (MQ=60)
                                       GAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCG                                                       >  SRR4302537.1609193/1‑94 (MQ=60)
                                        AAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGG                                                      <  SRR4302537.5685911/94‑1 (MQ=60)
                                        AAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGG                                                      <  SRR4302537.5944265/94‑1 (MQ=60)
                                        AAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGG                                                      >  SRR4302537.1056058/1‑94 (MQ=60)
                                         AAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGA                                                     >  SRR4302537.2253497/1‑94 (MQ=60)
                                          AAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGCA                                                    >  SRR4302537.1353578/1‑94 (MQ=60)
                                          AAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAA                                                    <  SRR4302537.1980769/94‑1 (MQ=60)
                                           AAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAA                                                   >  SRR4302537.1561183/1‑94 (MQ=60)
                                            AACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAT                                                  <  SRR4302537.333386/94‑1 (MQ=60)
                                            AACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAT                                                  <  SRR4302537.5797331/94‑1 (MQ=60)
                                            AACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAT                                                  >  SRR4302537.2014990/1‑94 (MQ=60)
                                             ACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATC                                                 <  SRR4302537.1342312/94‑1 (MQ=60)
                                             ACAGCAACTGCTGTACAACGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATC                                                 >  SRR4302537.2321270/1‑94 (MQ=60)
                                              CAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCG                                                >  SRR4302537.3451382/1‑94 (MQ=60)
                                              CAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCG                                                <  SRR4302537.4745442/94‑1 (MQ=60)
                                                GCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGAT                                              <  SRR4302537.2231630/94‑1 (MQ=60)
                                                GCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGAT                                              <  SRR4302537.2044459/94‑1 (MQ=60)
                                                 CAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATG                                             <  SRR4302537.4179734/94‑1 (MQ=60)
                                                 CAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATG                                             <  SRR4302537.4037345/94‑1 (MQ=60)
                                                 CAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATG                                             >  SRR4302537.1140944/1‑94 (MQ=60)
                                                 CAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATG                                             >  SRR4302537.5349345/1‑94 (MQ=60)
                                                   ACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGC                                           >  SRR4302537.2287209/1‑94 (MQ=60)
                                                    CTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCT                                          <  SRR4302537.1593417/94‑1 (MQ=60)
                                                     TGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTG                                         >  SRR4302537.5040748/1‑94 (MQ=60)
                                                      GCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGC                                        >  SRR4302537.2251850/1‑94 (MQ=60)
                                                      GCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGC                                        >  SRR4302537.5190126/1‑94 (MQ=60)
                                                       CTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCG                                       >  SRR4302537.1029788/1‑94 (MQ=60)
                                                       CTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCG                                       <  SRR4302537.4540299/94‑1 (MQ=60)
                                                       CTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCG                                       >  SRR4302537.1606703/1‑94 (MQ=60)
                                                       CTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCG                                       >  SRR4302537.3387698/1‑94 (MQ=60)
                                                        TGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGA                                      >  SRR4302537.3628245/1‑94 (MQ=60)
                                                         GTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAA                                     <  SRR4302537.1204287/94‑1 (MQ=60)
                                                           ACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGG                                   >  SRR4302537.3057319/1‑94 (MQ=60)
                                                            CACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGT                                  >  SRR4302537.932573/1‑94 (MQ=60)
                                                            CACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGT                                  >  SRR4302537.4725222/1‑94 (MQ=60)
                                                             ACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTT                                 >  SRR4302537.5400591/1‑94 (MQ=60)
                                                             ACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTA                                 >  SRR4302537.6303923/1‑94 (MQ=60)
                                                             ACCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTA                                 <  SRR4302537.29969/94‑1 (MQ=60)
                                                              CCGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTAT                                <  SRR4302537.4429392/94‑1 (MQ=60)
                                                               CGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATT                               >  SRR4302537.4063106/1‑94 (MQ=60)
                                                               CGTAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTGCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATT                               <  SRR4302537.700915/94‑1 (MQ=60)
                                                                 TAGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCT                             <  SRR4302537.1845983/94‑1 (MQ=60)
                                                                  AGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTC                            <  SRR4302537.2688222/94‑1 (MQ=60)
                                                                  AGCGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTC                            >  SRR4302537.2828037/1‑94 (MQ=60)
                                                                   GCGGAAAAAGGAGCAGAGAGCACCGGGCTGAAAATTATCGGATCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTCCCAAGGTATTCTCA                           >  SRR4302537.2236131/1‑94 (MQ=60)
                                                                    CGGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAA                          >  SRR4302537.178589/1‑94 (MQ=60)
                                                                     GGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAC                         <  SRR4302537.2586445/94‑1 (MQ=60)
                                                                     GGAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAC                         >  SRR4302537.1220038/1‑94 (MQ=60)
                                                                      GAAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACG                        <  SRR4302537.4966694/94‑1 (MQ=60)
                                                                       AAAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGC                       >  SRR4302537.2997160/1‑94 (MQ=60)
                                                                        AAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCG                      <  SRR4302537.4333738/94‑1 (MQ=60)
                                                                        AAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCG                      >  SRR4302537.2771164/1‑94 (MQ=60)
                                                                        AAAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCAATGGCTGCGAAGGTATTCTCAACGCG                      <  SRR4302537.2057101/94‑1 (MQ=60)
                                                                         AAAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGA                     <  SRR4302537.504466/94‑1 (MQ=60)
                                                                          AAGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGAT                    <  SRR4302537.3979986/94‑1 (MQ=60)
                                                                           AGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATG                   <  SRR4302537.3309347/94‑1 (MQ=60)
                                                                           AGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATG                   <  SRR4302537.4428457/94‑1 (MQ=60)
                                                                           AGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATG                   >  SRR4302537.5953091/1‑94 (MQ=60)
                                                                           AGGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATG                   >  SRR4302537.3516789/1‑94 (MQ=60)
                                                                            GGCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGG                  <  SRR4302537.5095477/94‑1 (MQ=60)
                                                                             GCGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGC                 >  SRR4302537.493604/1‑94 (MQ=60)
                                                                              CGCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCG                >  SRR4302537.506564/1‑94 (MQ=60)
                                                                               GCAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGC               >  SRR4302537.4095125/1‑94 (MQ=60)
                                                                                CAGAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCG              >  SRR4302537.2371853/1‑94 (MQ=60)
                                                                                  GAGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGCAGCGCTGCATACGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTC            >  SRR4302537.1014686/1‑94 (MQ=60)
                                                                                   AGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCC           >  SRR4302537.3710662/1‑94 (MQ=60)
                                                                                   AGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCC           >  SRR4302537.123420/1‑94 (MQ=60)
                                                                                   AGAGCACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCC           <  SRR4302537.568964/94‑1 (MQ=60)
                                                                                       CACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAA       <  SRR4302537.2597111/94‑1 (MQ=60)
                                                                                        ACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTTTCAACGCGATGGCGCGTCCGCAAA      >  SRR4302537.6512175/1‑94 (MQ=60)
                                                                                        ACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAA      >  SRR4302537.3344210/1‑94 (MQ=60)
                                                                                        ACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAA      >  SRR4302537.2832097/1‑94 (MQ=60)
                                                                                        ACCGGGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAA      >  SRR4302537.3420186/1‑94 (MQ=60)
                                                                                            GGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGC  <  SRR4302537.59230/94‑1 (MQ=60)
                                                                                            GGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGC  >  SRR4302537.5873802/1‑94 (MQ=60)
                                                                                            GGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGC  <  SRR4302537.2743557/94‑1 (MQ=60)
                                                                                            |                                                                                             
CCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGC  >  NC_000913/4550646‑4550831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑