Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,691,024 A→T F847Y (TTT→TAT)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,0240AT100.0% 1180.6 / NA 304F847Y (TTT→TAT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (146/158);  total (146/158)

Only 100 of 310 total aligned reads displayed.

CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113
                                                                                          |                                                                                         
cGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataa                                                                                         <  2:4812093/93‑1 (MQ=255)
cGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataa                                                                                         <  1:3151050/93‑1 (MQ=255)
 gCTGTGCAACCAGCTCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                                        <  2:3151030/93‑1 (MQ=255)
  cTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAc                                                                                       >  2:6256060/1‑93 (MQ=255)
     tgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTg                                                                                    <  1:857911/93‑1 (MQ=255)
     tgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTg                                                                                    <  1:5554892/93‑1 (MQ=255)
     tgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTg                                                                                    <  1:2566920/93‑1 (MQ=255)
        aaCCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcgc                                                                                 >  2:4164217/1‑93 (MQ=255)
          ccAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCa                                                                               <  2:573558/93‑1 (MQ=255)
           cAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAg                                                                              <  2:3960688/93‑1 (MQ=255)
           cAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAg                                                                              <  1:2238763/93‑1 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             >  1:3405054/1‑93 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             <  2:540959/93‑1 (MQ=255)
             gcgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcg                                                                            >  2:1876446/1‑93 (MQ=255)
              cgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcgc                                                                           <  2:371274/93‑1 (MQ=255)
               gcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGcc                                                                          >  2:4219319/1‑93 (MQ=255)
                ccTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCg                                                                         >  1:3209370/1‑93 (MQ=255)
                ccTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCg                                                                         >  1:4661790/1‑93 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  2:6872077/93‑1 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  1:3067359/93‑1 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  1:3808453/93‑1 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       >  2:5999874/1‑93 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       <  2:2526933/93‑1 (MQ=255)
                    aaTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                                     >  2:4538668/1‑93 (MQ=255)
                     aTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcg                                                                    >  2:160209/1‑93 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                                   <  1:2432600/93‑1 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                                   <  2:4273804/93‑1 (MQ=255)
                        gcgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGccc                                                                 >  1:1027124/1‑93 (MQ=255)
                         cgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa                                                                <  2:4484579/93‑1 (MQ=255)
                           tCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                                              >  1:279345/1‑93 (MQ=255)
                             aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                                            <  1:2198645/93‑1 (MQ=255)
                              tAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGcgcg                                                           <  1:4338079/93‑1 (MQ=255)
                              tAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGcgcg                                                           >  1:5249189/1‑93 (MQ=255)
                               aGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGt                                                          <  1:6502056/93‑1 (MQ=255)
                                gAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGtt                                                         >  1:2138118/1‑93 (MQ=255)
                                gAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGtt                                                         >  2:4680512/1‑93 (MQ=255)
                                 aaGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTa                                                        <  2:1275755/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  2:5652587/93‑1 (MQ=255)
                                   gCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAtt                                                      >  1:4406353/1‑93 (MQ=255)
                                   gCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAtt                                                      <  1:7406419/93‑1 (MQ=255)
                                    ccTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTg                                                     <  1:4099999/93‑1 (MQ=255)
                                    ccTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTg                                                     <  1:3521454/93‑1 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   <  2:2314692/93‑1 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   <  2:5840320/93‑1 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   >  1:3577597/1‑93 (MQ=255)
                                       ttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCt                                                  <  2:653803/93‑1 (MQ=255)
                                       ttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCt                                                  >  2:3216772/1‑93 (MQ=255)
                                        tCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                                                 <  2:531260/93‑1 (MQ=255)
                                        tCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                                                 <  1:6438679/93‑1 (MQ=255)
                                        tCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                                                 >  2:7297538/1‑93 (MQ=255)
                                         cAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttt                                                <  1:1917349/93‑1 (MQ=255)
                                           gTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttttc                                              >  2:5584196/1‑91 (MQ=255)
                                           gTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGc                                              >  2:1103814/1‑93 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             <  2:4050910/93‑1 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             >  2:1821800/1‑93 (MQ=255)
                                                  gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatc                                       >  1:1762901/1‑93 (MQ=255)
                                                   aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGTCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                                      >  1:1563818/1‑93 (MQ=255)
                                                   aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                                      >  1:6053708/1‑93 (MQ=255)
                                                    cATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaa                                     >  2:2976392/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   >  1:5519798/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   >  2:1319188/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   <  1:5037018/93‑1 (MQ=255)
                                                        gcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcag                                 <  2:2741462/93‑1 (MQ=255)
                                                        gcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcag                                 >  1:5427713/1‑93 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                <  2:1538712/93‑1 (MQ=255)
                                                           tACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcag                              >  2:7034989/1‑93 (MQ=255)
                                                            aCATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagc                             <  1:669854/93‑1 (MQ=255)
                                                            aCATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTGCCCCAAATCATTcggaagc                             >  1:4905538/1‑93 (MQ=255)
                                                             cATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagca                            <  2:5486122/93‑1 (MQ=255)
                                                              aTCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagcag                           <  2:1185541/93‑1 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          >  1:6886063/1‑93 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          <  2:1431020/93‑1 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          <  1:3346868/93‑1 (MQ=255)
                                                                 tGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc                        >  1:4715745/1‑93 (MQ=255)
                                                                 tGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc                        >  2:2138483/1‑93 (MQ=255)
                                                                  gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg                       >  1:5820935/1‑93 (MQ=255)
                                                                  gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg                       >  2:6472162/1‑93 (MQ=255)
                                                                    cGGTTTGTCGCCAGGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGtt                     <  2:2700288/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  1:2110102/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    >  1:6928828/1‑93 (MQ=255)
                                                                      gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAt                   >  1:1183348/1‑93 (MQ=255)
                                                                      gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAt                   <  1:3628649/93‑1 (MQ=255)
                                                                      gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAt                   <  1:6112500/93‑1 (MQ=255)
                                                                       tttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATc                  <  2:7194002/93‑1 (MQ=255)
                                                                        ttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCt                 >  2:6136646/1‑93 (MQ=255)
                                                                           tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCg              <  1:5126702/93‑1 (MQ=255)
                                                                            cGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCATTGCGTTATCTTCgg             <  2:5180317/93‑1 (MQ=255)
                                                                            cGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCgg             >  2:177150/1‑93 (MQ=255)
                                                                             gCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGt            >  2:5900450/1‑93 (MQ=255)
                                                                              ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           <  1:5332574/93‑1 (MQ=255)
                                                                              ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           >  1:3904094/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  2:412476/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  1:2616219/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  2:622727/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  1:7017853/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  2:3275902/1‑93 (MQ=255)
                                                                                aaGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGa         <  1:3002559/93‑1 (MQ=255)
                                                                                    tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGc     <  1:1362714/93‑1 (MQ=255)
                                                                                    tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGc     >  2:7495774/1‑93 (MQ=255)
                                                                                       cGATAAATCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAACCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTgc  <  2:3365663/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  >  NC_000913/2690932‑2691117
                                                                                            |                                                                                             
TACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATA                                                                                              >  SRR4302542.2735965/1‑94 (MQ=60)
  CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                            <  SRR4302542.3180973/94‑1 (MQ=60)
  CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                            <  SRR4302542.5494364/94‑1 (MQ=60)
     TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         >  SRR4302542.3439431/1‑94 (MQ=60)
     TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         >  SRR4302542.2627294/1‑94 (MQ=60)
     TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCCGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         <  SRR4302542.4879353/94‑1 (MQ=60)
      GTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTG                                                                                        <  SRR4302542.3294052/94‑1 (MQ=60)
      GTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTG                                                                                        <  SRR4302542.3947932/94‑1 (MQ=60)
       TGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGC                                                                                       <  SRR4302542.865303/94‑1 (MQ=60)
       TGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGC                                                                                       <  SRR4302542.2139989/94‑1 (MQ=60)
       TGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGC                                                                                       <  SRR4302542.5608862/94‑1 (MQ=60)
       TGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGC                                                                                       <  SRR4302542.2591797/94‑1 (MQ=60)
         CAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGC                                                                                     <  SRR4302542.5054315/94‑1 (MQ=60)
          AACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCC                                                                                    >  SRR4302542.224192/1‑94 (MQ=60)
           ACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCA                                                                                   <  SRR4302542.4743583/94‑1 (MQ=60)
            CCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAG                                                                                  <  SRR4302542.5590654/94‑1 (MQ=60)
             CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                 <  SRR4302542.2258018/94‑1 (MQ=60)
             CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                 >  SRR4302542.3436975/1‑94 (MQ=60)
             CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                 >  SRR4302542.1222928/1‑94 (MQ=60)
              AGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCG                                                                                >  SRR4302542.2616459/1‑94 (MQ=60)
                CGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCC                                                                              >  SRR4302542.5454748/1‑94 (MQ=60)
                 GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302542.4707364/1‑94 (MQ=60)
                 GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302542.3239758/1‑94 (MQ=60)
                 GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302542.2240560/1‑94 (MQ=60)
                 GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302542.4747763/1‑94 (MQ=60)
                   CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302542.3846136/94‑1 (MQ=60)
                   CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302542.3096605/94‑1 (MQ=60)
                   CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302542.2432341/94‑1 (MQ=60)
                    TGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTG                                                                          >  SRR4302542.3772885/1‑94 (MQ=60)
                    TGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTG                                                                          <  SRR4302542.1631248/94‑1 (MQ=60)
                      AATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCG                                                                        <  SRR4302542.5370508/94‑1 (MQ=60)
                        TCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCC                                                                      <  SRR4302542.2456272/94‑1 (MQ=60)
                         CGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCC                                                                     >  SRR4302542.1035976/1‑94 (MQ=60)
                         CGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCC                                                                     >  SRR4302542.2212672/1‑94 (MQ=60)
                         CGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCAGCGCCAGCGCCGTTGCGCCC                                                                     >  SRR4302542.3788750/1‑94 (MQ=60)
                          GCGTCATAGAAGCCTTTCAGTTTCGCGACATCGCGGACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCC                                                                    >  SRR4302542.3708679/1‑94 (MQ=60)
                            GTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGC                                                                  <  SRR4302542.5088256/94‑1 (MQ=60)
                            GTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGC                                                                  >  SRR4302542.281776/1‑94 (MQ=60)
                            GTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGC                                                                  <  SRR4302542.3386977/94‑1 (MQ=60)
                               ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                               <  SRR4302542.2217577/94‑1 (MQ=60)
                               ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                               >  SRR4302542.5300214/1‑94 (MQ=60)
                               ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                               <  SRR4302542.436803/94‑1 (MQ=60)
                                TAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGT                                                              <  SRR4302542.4380708/94‑1 (MQ=60)
                                 AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             <  SRR4302542.6566144/94‑1 (MQ=60)
                                 AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             >  SRR4302542.2121500/1‑94 (MQ=60)
                                 AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             >  SRR4302542.3171236/1‑94 (MQ=60)
                                 AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             >  SRR4302542.2156566/1‑94 (MQ=60)
                                  GAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTA                                                            >  SRR4302542.3635488/1‑94 (MQ=60)
                                  GAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTA                                                            >  SRR4302542.3197718/1‑94 (MQ=60)
                                   AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           >  SRR4302542.5880586/1‑94 (MQ=60)
                                   AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           <  SRR4302542.2954861/94‑1 (MQ=60)
                                   AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           <  SRR4302542.3911410/94‑1 (MQ=60)
                                   AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           >  SRR4302542.6473347/1‑94 (MQ=60)
                                   AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           <  SRR4302542.2151886/94‑1 (MQ=60)
                                    AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302542.4129709/94‑1 (MQ=60)
                                    AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302542.6542875/94‑1 (MQ=60)
                                    AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          >  SRR4302542.4449626/1‑94 (MQ=60)
                                    AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302542.6973002/94‑1 (MQ=60)
                                     GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                         <  SRR4302542.4823654/94‑1 (MQ=60)
                                     GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                         <  SRR4302542.7478905/94‑1 (MQ=60)
                                     GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                         <  SRR4302542.918801/94‑1 (MQ=60)
                                      CCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGC                                                        <  SRR4302542.4140430/94‑1 (MQ=60)
                                      CCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGC                                                        <  SRR4302542.3554364/94‑1 (MQ=60)
                                       CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       >  SRR4302542.1455236/1‑94 (MQ=60)
                                       CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       >  SRR4302542.3610981/1‑94 (MQ=60)
                                       CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       >  SRR4302542.3211454/1‑94 (MQ=60)
                                       CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       <  SRR4302542.5685443/94‑1 (MQ=60)
                                       CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       <  SRR4302542.5193865/94‑1 (MQ=60)
                                         TTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTT                                                     >  SRR4302542.3847783/1‑94 (MQ=60)
                                         TTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTT                                                     <  SRR4302542.5159847/94‑1 (MQ=60)
                                         TTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTT                                                     <  SRR4302542.5258454/94‑1 (MQ=60)
                                         TTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTT                                                     <  SRR4302542.3402239/94‑1 (MQ=60)
                                          TCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTT                                                    <  SRR4302542.6502104/94‑1 (MQ=60)
                                           CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302542.3174816/94‑1 (MQ=60)
                                           CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302542.1933917/94‑1 (MQ=60)
                                           CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302542.4634316/94‑1 (MQ=60)
                                           CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302542.3010812/94‑1 (MQ=60)
                                              TTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCC                                                <  SRR4302542.424304/94‑1 (MQ=60)
                                                 CGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAA                                             >  SRR4302542.2492466/1‑94 (MQ=60)
                                                  GCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAAT                                            <  SRR4302542.2450999/94‑1 (MQ=60)
                                                   CGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATC                                           <  SRR4302542.5503052/94‑1 (MQ=60)
                                                   CGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATC                                           >  SRR4302542.1778192/1‑94 (MQ=60)
                                                    GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGTCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          >  SRR4302542.1577330/1‑94 (MQ=60)
                                                    GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          >  SRR4302542.6112298/1‑94 (MQ=60)
                                                    GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          >  SRR4302542.5008355/1‑94 (MQ=60)
                                                    GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          >  SRR4302542.613299/1‑94 (MQ=60)
                                                    GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          <  SRR4302542.5667050/94‑1 (MQ=60)
                                                      CATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCAAAATCAAT                                        <  SRR4302542.2097956/94‑1 (MQ=60)
                                                       ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                       >  SRR4302542.7055549/1‑94 (MQ=60)
                                                       ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                       <  SRR4302542.2474847/94‑1 (MQ=60)
                                                       ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                       >  SRR4302542.5573443/1‑94 (MQ=60)
                                                        TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                      <  SRR4302542.7594267/94‑1 (MQ=60)
                                                        TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                      <  SRR4302542.5085919/94‑1 (MQ=60)
                                                         CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                     >  SRR4302542.2201583/1‑94 (MQ=60)
                                                         CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                     <  SRR4302542.3559742/94‑1 (MQ=60)
                                                         CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                     >  SRR4302542.5480457/1‑94 (MQ=60)
                                                          GCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGC                                    <  SRR4302542.1087498/94‑1 (MQ=60)
                                                           CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                   >  SRR4302542.3074405/1‑94 (MQ=60)
                                                            GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  <  SRR4302542.2415147/94‑1 (MQ=60)
                                                             TACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGC                                 <  SRR4302542.2806156/94‑1 (MQ=60)
                                                             TACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTGCCCCAAATCAttcggaagc                                 >  SRR4302542.4953255/1‑85 (MQ=60)
                                                              ACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCA                                >  SRR4302542.4391708/1‑94 (MQ=60)
                                                              ACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCA                                <  SRR4302542.6130151/94‑1 (MQ=60)
                                                              ACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCA                                <  SRR4302542.675638/94‑1 (MQ=60)
                                                               CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                               <  SRR4302542.7186860/94‑1 (MQ=60)
                                                               CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                               >  SRR4302542.1442499/1‑94 (MQ=60)
                                                               CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                               >  SRR4302542.3626107/1‑94 (MQ=60)
                                                                ATCTGCTGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              >  SRR4302542.6158662/1‑94 (MQ=60)
                                                                ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCTCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              <  SRR4302542.6270622/94‑1 (MQ=60)
                                                                ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              <  SRR4302542.651428/94‑1 (MQ=60)
                                                                ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              >  SRR4302542.4697884/1‑94 (MQ=60)
                                                                ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              >  SRR4302542.6953697/1‑94 (MQ=60)
                                                                ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              >  SRR4302542.6654623/1‑94 (MQ=60)
                                                                 TCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                             <  SRR4302542.3378367/94‑1 (MQ=60)
                                                                  CTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGC                            >  SRR4302542.6977987/1‑94 (MQ=60)
                                                                  CTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGC                            >  SRR4302542.3260627/1‑94 (MQ=60)
                                                                  CTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGC                            >  SRR4302542.4761715/1‑94 (MQ=60)
                                                                   TGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCG                           >  SRR4302542.5877451/1‑94 (MQ=60)
                                                                    GCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGT                          >  SRR4302542.7374944/1‑94 (MQ=60)
                                                                    GCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCAT                          <  SRR4302542.5479561/94‑1 (MQ=60)
                                                                     CCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTT                         >  SRR4302542.1757592/1‑94 (MQ=60)
                                                                      CGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTA                        >  SRR4302542.6996855/1‑94 (MQ=60)
                                                                      CGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTA                        >  SRR4302542.443066/1‑94 (MQ=60)
                                                                       GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                       >  SRR4302542.1193555/1‑94 (MQ=60)
                                                                       GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                       <  SRR4302542.2128314/94‑1 (MQ=60)
                                                                        GTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATC                      <  SRR4302542.3662457/94‑1 (MQ=60)
                                                                        GTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATC                      <  SRR4302542.6171651/94‑1 (MQ=60)
                                                                          TTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTT                    <  SRR4302542.5977086/94‑1 (MQ=60)
                                                                            GTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCG                  >  SRR4302542.3608214/1‑94 (MQ=60)
                                                                            GTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCG                  <  SRR4302542.7013140/94‑1 (MQ=60)
                                                                             TCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGG                 <  SRR4302542.5176485/94‑1 (MQ=60)
                                                                             TCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGG                 >  SRR4302542.3463279/1‑94 (MQ=60)
                                                                              CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCtcttctttttccacgaccgggtc                >  SRR4302542.4377685/1‑71 (MQ=60)
                                                                              CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGT                >  SRR4302542.1875581/1‑94 (MQ=60)
                                                                               GCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTA               >  SRR4302542.3942634/1‑94 (MQ=60)
                                                                               GCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTA               >  SRR4302542.6777759/1‑94 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              >  SRR4302542.2641494/1‑94 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              <  SRR4302542.2751028/94‑1 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              >  SRR4302542.7086819/1‑94 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              <  SRR4302542.5384388/94‑1 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              >  SRR4302542.5303840/1‑94 (MQ=60)
                                                                                CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG              >  SRR4302542.5579778/1‑94 (MQ=60)
                                                                                 CAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGA             >  SRR4302542.1840933/1‑94 (MQ=60)
                                                                                  AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA            <  SRR4302542.3031273/94‑1 (MQ=60)
                                                                                  AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA            >  SRR4302542.3081814/1‑94 (MQ=60)
                                                                                   AGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAA           <  SRR4302542.6903949/94‑1 (MQ=60)
                                                                                   AGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAA           >  SRR4302542.1264644/1‑94 (MQ=60)
                                                                                     CTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGC         >  SRR4302542.6687585/1‑94 (MQ=60)
                                                                                      TGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCT        <  SRR4302542.1374497/94‑1 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     >  SRR4302542.3420239/1‑94 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302542.1089387/94‑1 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302542.7522371/94‑1 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302542.3463970/94‑1 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     >  SRR4302542.1647639/1‑94 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     >  SRR4302542.5278943/1‑94 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     >  SRR4302542.5884499/1‑94 (MQ=60)
                                                                                         CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302542.4823458/94‑1 (MQ=60)
                                                                                          GATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGG    >  SRR4302542.6967449/1‑94 (MQ=60)
                                                                                           ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC   <  SRR4302542.1856059/94‑1 (MQ=60)
                                                                                           ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC   >  SRR4302542.1792883/1‑94 (MQ=60)
                                                                                           ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC   >  SRR4302542.2049167/1‑94 (MQ=60)
                                                                                            TAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  <  SRR4302542.1520634/94‑1 (MQ=60)
                                                                                            |                                                                                             
TACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  >  NC_000913/2690932‑2691117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑