Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,853,930 G→A D524N (GAT→AAT)  fhlA → FhlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,853,9300GA87.6% 757.5 / 60.0 259D524N (GAT→AAT) fhlAFhlA
Reads supporting (aligned to +/- strand):  ref base G (16/16);  new base A (124/103);  total (140/119)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 262 total aligned reads displayed.

GCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGA  >  NC_000913/2853839‑2854021
                                                                                           |                                                                                           
gctgcGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGa                                                                                            <  1:6494977/93‑1 (MQ=255)
 ctgcGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAAt                                                                                           <  2:4993607/93‑1 (MQ=255)
  tgcGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATc                                                                                          <  1:5891047/93‑1 (MQ=255)
  tgcGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATc                                                                                          >  2:5592123/1‑93 (MQ=255)
    cGTGTATTCCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTg                                                                                        >  1:320124/1‑93 (MQ=255)
     gtgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTTTAATCGCCGCGACTAACCGCAATCTGa                                                                                       >  2:2182677/1‑93 (MQ=255)
     gtgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGa                                                                                       >  1:875279/1‑93 (MQ=255)
     gtgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGa                                                                                       <  1:2635577/93‑1 (MQ=255)
      tgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaa                                                                                      >  1:4384245/1‑93 (MQ=255)
      tgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaa                                                                                      <  1:56153/93‑1 (MQ=255)
      tgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaa                                                                                      >  2:6751671/1‑93 (MQ=255)
      tgtATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaa                                                                                      <  1:2278297/93‑1 (MQ=255)
        tATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGaaaa                                                                                    >  2:2034308/1‑93 (MQ=255)
        tATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaaaa                                                                                    >  2:3149251/1‑93 (MQ=255)
        tATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaaaa                                                                                    >  2:1842362/1‑93 (MQ=255)
           tGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaaaaaaa                                                                                 <  1:3770423/93‑1 (MQ=255)
           tGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGaaaaaaa                                                                                 <  2:4683336/93‑1 (MQ=255)
            gCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAAt                                                                                >  2:6355950/1‑93 (MQ=255)
            gCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAAt                                                                                <  2:719575/93‑1 (MQ=255)
            gCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAAt                                                                                <  2:3993335/93‑1 (MQ=255)
               ggAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGt                                                                             <  1:2921492/93‑1 (MQ=255)
                gAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTc                                                                            <  2:6849240/93‑1 (MQ=255)
                 aaCAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCg                                                                           >  1:6912191/1‑93 (MQ=255)
                  aCAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGc                                                                          <  1:63244/93‑1 (MQ=255)
                  aCAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGc                                                                          >  1:6707262/1‑93 (MQ=255)
                  aCAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGc                                                                          <  2:7308547/93‑1 (MQ=255)
                   cAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGcc                                                                         >  1:452150/1‑93 (MQ=255)
                   cAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGcc                                                                         <  2:1169011/93‑1 (MQ=255)
                     ggAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTTCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGa                                                                       >  1:3393103/1‑93 (MQ=255)
                     ggAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGa                                                                       >  1:3669313/1‑93 (MQ=255)
                     ggAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGa                                                                       >  1:186786/1‑93 (MQ=255)
                     ggAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGa                                                                       >  1:5335386/1‑93 (MQ=255)
                     ggAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGa                                                                       >  1:5020511/1‑93 (MQ=255)
                      gAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGAc                                                                      <  2:2843205/93‑1 (MQ=255)
                         tttGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGt                                                                   <  2:5159840/93‑1 (MQ=255)
                           tGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGa                                                                 <  2:4362714/93‑1 (MQ=255)
                           tGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGa                                                                 >  2:3387603/1‑93 (MQ=255)
                           tGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGa                                                                 <  1:2010456/93‑1 (MQ=255)
                           tGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGa                                                                 >  2:7015390/1‑93 (MQ=255)
                              aCGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAggt                                                              >  2:579344/1‑91 (MQ=255)
                              aCGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGtt                                                              >  2:2756354/1‑93 (MQ=255)
                                gTCTCGGCAGCAACAAAATCATTCAGACGGCCGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTcc                                                            >  1:6456385/1‑93 (MQ=255)
                                gTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTcc                                                            >  1:3023749/1‑93 (MQ=255)
                                gTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTcc                                                            >  2:1550755/1‑93 (MQ=255)
                                   tcGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTa                                                         <  1:5853830/93‑1 (MQ=255)
                                   tcGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTa                                                         <  1:1565811/93‑1 (MQ=255)
                                      gcagcaACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCg                                                      <  2:1880616/93‑1 (MQ=255)
                                       cagcaACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGa                                                     <  1:1187480/93‑1 (MQ=255)
                                        agcaACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAt                                                    >  2:4477974/1‑93 (MQ=255)
                                         gcaACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAtc                                                   <  1:7259389/93‑1 (MQ=255)
                                          caacaaAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAtct                                                  >  1:733549/1‑93 (MQ=255)
                                           aacaaAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAtctc                                                 >  2:5492273/1‑93 (MQ=255)
                                           aacaaAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAtctc                                                 >  1:6812263/1‑93 (MQ=255)
                                            acaaAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATctct                                                >  1:5562112/1‑93 (MQ=255)
                                             caaAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTa                                               <  2:6463597/93‑1 (MQ=255)
                                                aaTCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTa                                            <  1:3363578/93‑1 (MQ=255)
                                                 aTCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTAc                                           <  2:4764439/93‑1 (MQ=255)
                                                   cATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCg                                         <  1:4047214/93‑1 (MQ=255)
                                                    aTTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGc                                        >  1:4270928/1‑93 (MQ=255)
                                                     ttCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGcc                                       >  1:4978582/1‑93 (MQ=255)
                                                     ttCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGcc                                       >  1:4572337/1‑93 (MQ=255)
                                                           cggacgTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACg                                 >  1:4745081/1‑93 (MQ=255)
                                                             gacgTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTa                               <  1:2047264/93‑1 (MQ=255)
                                                               cgTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTAtt                             <  2:5266586/93‑1 (MQ=255)
                                                                gTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTc                            <  2:243469/93‑1 (MQ=255)
                                                                 tGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTcc                           <  1:211678/93‑1 (MQ=255)
                                                                 tGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTcc                           <  1:1384626/93‑1 (MQ=255)
                                                                 tGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTcc                           <  1:2709796/93‑1 (MQ=255)
                                                                 tGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTcc                           <  1:6099312/93‑1 (MQ=255)
                                                                 tGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTcc                           >  2:429635/1‑93 (MQ=255)
                                                                  gCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTccc                          >  2:788184/1‑93 (MQ=255)
                                                                  gCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTccc                          <  2:3323769/93‑1 (MQ=255)
                                                                  gCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTccc                          <  2:1705142/93‑1 (MQ=255)
                                                                   cGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCg                         >  2:7359484/1‑93 (MQ=255)
                                                                    gTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGa                        <  2:4168858/93‑1 (MQ=255)
                                                                     tCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAt                       >  1:4163415/1‑93 (MQ=255)
                                                                     tCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAt                       >  1:2587435/1‑93 (MQ=255)
                                                                     tCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAt                       >  2:7170109/1‑93 (MQ=255)
                                                                     tCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAt                       >  1:796358/1‑93 (MQ=255)
                                                                      cTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAtt                      >  1:1674174/1‑93 (MQ=255)
                                                                      cTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAtt                      <  1:3593177/93‑1 (MQ=255)
                                                                       tAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTc                     <  2:2688856/93‑1 (MQ=255)
                                                                        aaTCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCa                    <  1:5119793/93‑1 (MQ=255)
                                                                        aaTCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCa                    >  1:3879436/1‑93 (MQ=255)
                                                                        aaTCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCa                    >  2:1290041/1‑93 (MQ=255)
                                                                         aTCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCAc                   <  2:5084833/93‑1 (MQ=255)
                                                                         aTCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCAc                   <  1:1016584/93‑1 (MQ=255)
                                                                          tCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCAcc                  >  1:2387163/1‑93 (MQ=255)
                                                                           cgccgcGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCt                 <  1:7359552/93‑1 (MQ=255)
                                                                             ccgcGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTgc               >  1:1940629/1‑93 (MQ=255)
                                                                               gcgACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTgccg             >  2:589647/1‑93 (MQ=255)
                                                                               gcgACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTgccg             >  2:7261651/1‑93 (MQ=255)
                                                                                 gACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTgccgcc           >  2:6014842/1‑93 (MQ=255)
                                                                                 gACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTgccgcc           <  2:1752245/93‑1 (MQ=255)
                                                                                  aCTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCa          >  1:2185669/1‑93 (MQ=255)
                                                                                   cTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCCCCTGCCGCCAc         <  1:2792001/93‑1 (MQ=255)
                                                                                   cTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCAc         <  2:1043471/93‑1 (MQ=255)
                                                                                   cTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCAc         <  1:624860/93‑1 (MQ=255)
                                                                                      aCCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTAc      >  2:2997120/1‑93 (MQ=255)
                                                                                      aCCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTAc      >  2:3481636/1‑93 (MQ=255)
                                                                                           |                                                                                           
GCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGA  >  NC_000913/2853839‑2854021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGC  >  NC_000913/2853839‑2854023
                                                                                           |                                                                                             
GCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGAT                                                                                             <  SRR4302542.6558980/94‑1 (MQ=60)
 CTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATC                                                                                            <  SRR4302542.5157341/94‑1 (MQ=60)
  TGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCT                                                                                           <  SRR4302542.5948136/94‑1 (MQ=60)
   GCGTGTATTCCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTG                                                                                          >  SRR4302542.322900/1‑94 (MQ=60)
    CGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGA                                                                                         >  SRR4302542.882831/1‑94 (MQ=60)
     GTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAA                                                                                        <  SRR4302542.3361868/94‑1 (MQ=60)
     GTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAA                                                                                        <  SRR4302542.2661030/94‑1 (MQ=60)
     GTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAA                                                                                        >  SRR4302542.4427265/1‑94 (MQ=60)
      TGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAA                                                                                       <  SRR4302542.56612/94‑1 (MQ=60)
      TGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAA                                                                                       <  SRR4302542.2297884/94‑1 (MQ=60)
       GTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAA                                                                                      >  SRR4302542.6793834/1‑94 (MQ=60)
        TATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAA                                                                                     >  SRR4302542.817882/1‑94 (MQ=60)
         ATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAA                                                                                    >  SRR4302542.3638232/1‑94 (MQ=60)
           TGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAAT                                                                                  <  SRR4302542.3807707/94‑1 (MQ=60)
             CAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGG                                                                                <  SRR4302542.4939494/94‑1 (MQ=60)
              AGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGT                                                                               >  SRR4302542.4174682/1‑94 (MQ=60)
               GGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTC                                                                              >  SRR4302542.3087008/1‑94 (MQ=60)
               GGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTC                                                                              <  SRR4302542.2949490/94‑1 (MQ=60)
               GGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTC                                                                              >  SRR4302542.2679230/1‑94 (MQ=60)
               GGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTC                                                                              <  SRR4302542.2007842/94‑1 (MQ=60)
               GGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTC                                                                              <  SRR4302542.5244963/94‑1 (MQ=60)
                GAACAGGAGTTTGAGCGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCC                                                                             <  SRR4302542.2400814/94‑1 (MQ=60)
                GAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCG                                                                             <  SRR4302542.5053786/94‑1 (MQ=60)
                GAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCG                                                                             >  SRR4302542.6980051/1‑94 (MQ=60)
                 AACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGC                                                                            >  SRR4302542.6773334/1‑94 (MQ=60)
                  ACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCC                                                                           >  SRR4302542.456056/1‑94 (MQ=60)
                  ACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCC                                                                           <  SRR4302542.63758/94‑1 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTTCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.3424946/1‑94 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.5622804/1‑94 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.5387228/1‑94 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.5069262/1‑94 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.3704491/1‑94 (MQ=60)
                    AGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGA                                                                         >  SRR4302542.188372/1‑94 (MQ=60)
                      GAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACC                                                                       <  SRR4302542.6155952/94‑1 (MQ=60)
                       AGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCG                                                                      >  SRR4302542.5858224/1‑94 (MQ=60)
                        GTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGT                                                                     >  SRR4302542.6329234/1‑94 (MQ=60)
                         TTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTG                                                                    <  SRR4302542.4530636/94‑1 (MQ=60)
                           TGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAG                                                                  <  SRR4302542.2027817/94‑1 (MQ=60)
                            GAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGT                                                                 >  SRR4302542.2824502/1‑94 (MQ=60)
                            GAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGT                                                                 >  SRR4302542.1633124/1‑94 (MQ=60)
                             AACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTT                                                                >  SRR4302542.6254625/1‑94 (MQ=60)
                             AACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTT                                                                >  SRR4302542.2150452/1‑94 (MQ=60)
                              ACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTC                                                               <  SRR4302542.4785423/94‑1 (MQ=60)
                               CGTCTCGGCAGCAACAAAATCATTCAGACGGCCGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCC                                                              >  SRR4302542.6519955/1‑94 (MQ=60)
                               CGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCC                                                              >  SRR4302542.3052615/1‑94 (MQ=60)
                               CGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCC                                                              >  SRR4302542.1740768/1‑94 (MQ=60)
                                  CTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTA                                                           <  SRR4302542.2282139/94‑1 (MQ=60)
                                   TCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAG                                                          <  SRR4302542.5910602/94‑1 (MQ=60)
                                   TCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAG                                                          <  SRR4302542.1688302/94‑1 (MQ=60)
                                   TCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAG                                                          <  SRR4302542.1579333/94‑1 (MQ=60)
                                     GGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCG                                                        <  SRR4302542.6904223/94‑1 (MQ=60)
                                     GGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCG                                                        >  SRR4302542.6525217/1‑94 (MQ=60)
                                       CAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGAT                                                      <  SRR4302542.1197714/94‑1 (MQ=60)
                                        AGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATC                                                     >  SRR4302542.5628254/1‑94 (MQ=60)
                                         GCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCT                                                    <  SRR4302542.3270426/94‑1 (MQ=60)
                                         GCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCT                                                    >  SRR4302542.739907/1‑94 (MQ=60)
                                         GCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCT                                                    <  SRR4302542.7330558/94‑1 (MQ=60)
                                          CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTC                                                   >  SRR4302542.5913503/1‑94 (MQ=60)
                                          CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTC                                                   >  SRR4302542.3954866/1‑94 (MQ=60)
                                          CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTC                                                   >  SRR4302542.6879231/1‑94 (MQ=60)
                                           AACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCT                                                  >  SRR4302542.5616156/1‑94 (MQ=60)
                                           AACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCT                                                  >  SRR4302542.341627/1‑94 (MQ=60)
                                           AACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCT                                                  >  SRR4302542.1976503/1‑94 (MQ=60)
                                            ACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTA                                                 >  SRR4302542.7418114/1‑94 (MQ=60)
                                             CAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTAT                                                <  SRR4302542.2315625/94‑1 (MQ=60)
                                             CAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTAT                                                <  SRR4302542.1361799/94‑1 (MQ=60)
                                              AAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATT                                               >  SRR4302542.2140432/1‑94 (MQ=60)
                                              AAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATT                                               <  SRR4302542.7170355/94‑1 (MQ=60)
                                               AAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTA                                              >  SRR4302542.3722046/1‑94 (MQ=60)
                                               AAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTA                                              >  SRR4302542.5524511/1‑94 (MQ=60)
                                                AATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTAC                                             <  SRR4302542.6264268/94‑1 (MQ=60)
                                                AATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTAC                                             <  SRR4302542.3395180/94‑1 (MQ=60)
                                                  TCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCG                                           >  SRR4302542.5029300/1‑94 (MQ=60)
                                                   CATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGC                                          <  SRR4302542.4087139/94‑1 (MQ=60)
                                                   CATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGC                                          >  SRR4302542.2063115/1‑94 (MQ=60)
                                                   CATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGC                                          >  SRR4302542.4312953/1‑94 (MQ=60)
                                                    ATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCC                                         >  SRR4302542.5026910/1‑94 (MQ=60)
                                                    ATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCC                                         >  SRR4302542.4586275/1‑94 (MQ=60)
                                                    ATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCC                                         >  SRR4302542.4617079/1‑94 (MQ=60)
                                                    ATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCC                                         >  SRR4302542.4617080/1‑94 (MQ=60)
                                                    ATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCC                                         <  SRR4302542.1507764/94‑1 (MQ=60)
                                                       CAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGA                                      >  SRR4302542.3655479/1‑94 (MQ=60)
                                                         GACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAAC                                    <  SRR4302542.3028812/94‑1 (MQ=60)
                                                         GACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAAC                                    >  SRR4302542.2955930/1‑94 (MQ=60)
                                                         GACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAAC                                    <  SRR4302542.5344761/94‑1 (MQ=60)
                                                          ACGGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACG                                   >  SRR4302542.4791330/1‑94 (MQ=60)
                                                           CGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGT                                  <  SRR4302542.425521/94‑1 (MQ=60)
                                                            GGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTA                                 >  SRR4302542.4522398/1‑94 (MQ=60)
                                                            GGACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTA                                 >  SRR4302542.1251755/1‑94 (MQ=60)
                                                             GACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTAT                                <  SRR4302542.2064960/94‑1 (MQ=60)
                                                             GACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTAT                                <  SRR4302542.3621609/94‑1 (MQ=60)
                                                             GACGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTAT                                <  SRR4302542.648648/94‑1 (MQ=60)
                                                               CGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTC                              >  SRR4302542.6125193/1‑94 (MQ=60)
                                                               CGTGCGTCTAATCGCCGCGACTAACCGCAGTCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTC                              >  SRR4302542.6796153/1‑94 (MQ=60)
                                                               CGTGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTC                              >  SRR4302542.423777/1‑94 (MQ=60)
                                                                 TGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCC                            <  SRR4302542.1396587/94‑1 (MQ=60)
                                                                 TGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCC                            <  SRR4302542.213499/94‑1 (MQ=60)
                                                                 TGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCC                            <  SRR4302542.6158340/94‑1 (MQ=60)
                                                                 TGCGTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCC                            <  SRR4302542.2735927/94‑1 (MQ=60)
                                                                    GTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAT                         <  SRR4302542.1917475/94‑1 (MQ=60)
                                                                    GTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAT                         >  SRR4302542.803207/1‑94 (MQ=60)
                                                                    GTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAT                         >  SRR4302542.2612506/1‑94 (MQ=60)
                                                                    GTCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGAT                         >  SRR4302542.4204468/1‑94 (MQ=60)
                                                                     TCTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATT                        >  SRR4302542.1688641/1‑94 (MQ=60)
                                                                      CTAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTC                       <  SRR4302542.3626686/94‑1 (MQ=60)
                                                                       TAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCA                      >  SRR4302542.3917757/1‑94 (MQ=60)
                                                                       TAATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCA                      <  SRR4302542.7570365/94‑1 (MQ=60)
                                                                        AATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCAC                     <  SRR4302542.5169510/94‑1 (MQ=60)
                                                                        AATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCAC                     >  SRR4302542.4036928/1‑94 (MQ=60)
                                                                         ATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACC                    >  SRR4302542.1085930/1‑94 (MQ=60)
                                                                         ATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACC                    <  SRR4302542.1025339/94‑1 (MQ=60)
                                                                         ATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACC                    >  SRR4302542.2076300/1‑94 (MQ=60)
                                                                         ATCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACC                    >  SRR4302542.2409447/1‑94 (MQ=60)
                                                                          TCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCT                   <  SRR4302542.6738042/94‑1 (MQ=60)
                                                                          TCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCT                   <  SRR4302542.1876/94‑1 (MQ=60)
                                                                          TCGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCT                   <  SRR4302542.2712903/94‑1 (MQ=60)
                                                                           CGCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTG                  <  SRR4302542.7431644/94‑1 (MQ=60)
                                                                            GCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGC                 >  SRR4302542.6450731/1‑94 (MQ=60)
                                                                            GCCGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGC                 >  SRR4302542.1957409/1‑94 (MQ=60)
                                                                              CGCGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCG               <  SRR4302542.2101618/94‑1 (MQ=60)
                                                                                CGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCC             >  SRR4302542.3816588/1‑94 (MQ=60)
                                                                                CGACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCC             >  SRR4302542.7018041/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.2204494/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.2636700/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.254680/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.3402524/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.956359/1‑94 (MQ=60)
                                                                                 GACTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGATCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCA            >  SRR4302542.7584789/1‑94 (MQ=60)
                                                                                   CTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCCCCTGCCGCCACT          <  SRR4302542.2818872/94‑1 (MQ=60)
                                                                                   CTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACT          >  SRR4302542.7400255/1‑94 (MQ=60)
                                                                                   CTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACT          >  SRR4302542.2935451/1‑94 (MQ=60)
                                                                                   CTAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACT          <  SRR4302542.630241/94‑1 (MQ=60)
                                                                                   CTAACCGCAATCTGAAAAAAATGGTCGCCGACCGGGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACT          >  SRR4302542.566397/1‑94 (MQ=60)
                                                                                    TAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTA         >  SRR4302542.2760958/1‑94 (MQ=60)
                                                                                    TAACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTA         >  SRR4302542.6590939/1‑94 (MQ=60)
                                                                                     AACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTAC        >  SRR4302542.252906/1‑94 (MQ=60)
                                                                                     AACCGCAATCTGAAAAAAATGGTCGCCGCCCGTGAGTTCCGTAGCAATCTCTATTACCGCCTGAACGTATTCCCGATTCCCCTGCCGCCACTAC        <  SRR4302542.7350847/94‑1 (MQ=60)
                                                                                      ACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACG       >  SRR4302542.3841517/1‑94 (MQ=60)
                                                                                      ACCGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACG       <  SRR4302542.5003895/94‑1 (MQ=60)
                                                                                        CGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCG     >  SRR4302542.4390139/1‑94 (MQ=60)
                                                                                        CGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCG     >  SRR4302542.3756379/1‑94 (MQ=60)
                                                                                        CGCAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCG     >  SRR4302542.3398230/1‑94 (MQ=60)
                                                                                         GCAATCTAAAAAAAATGGTCGCCGCCCGTGAGTTCCGTAGCGATCTCTATTACGGCCTGAACTTATTCCCGATCCCCCTGCCGCCACTACGCGA    <  SRR4302542.6076065/94‑1 (MQ=60)
                                                                                          CAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAG   >  SRR4302542.2280957/1‑94 (MQ=60)
                                                                                          CAATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAG   >  SRR4302542.5049569/1‑94 (MQ=60)
                                                                                           AATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGC  >  SRR4302542.5285194/1‑94 (MQ=60)
                                                                                           AATCTGAAAAAAAGGGCCGCCGCCCGTGAGTCCCGTAGCGATCTCTTTTCCCGCCTGAACGTTTTCCCGATTCCCCTGCCGCCACTACGCGAGC  <  SRR4302542.4384946/94‑1 (MQ=60)
                                                                                           AATCTAAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGTTCTCTTTTCCCGCCTGAACGTTTTCCCGATTCCCCTGCCGCCACTACGCGAGC  <  SRR4302542.6093263/94‑1 (MQ=60)
                                                                                           AATCTAAAAAAAATGGCCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCCCCTGCCGCCACTACGCGAGC  <  SRR4302542.5487036/94‑1 (MQ=60)
                                                                                           |                                                                                             
GCTGCGTGTATTGCAGGAACAGGAGTTTGAACGTCTCGGCAGCAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGC  >  NC_000913/2853839‑2854023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑