Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,119	C→T	R587C (CGC→TGC)	fhlA →	FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,119	0	C	T	84.0%	767.9 / 98.3	293	R587C (CGC→TGC)	fhlA	FhlA
Reads supporting (aligned to +/- strand): ref base C (24/23); new base T (141/105); total (165/128)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 296 total aligned reads displayed.

CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGC  >  NC_000913/2854027‑2854209

   aaGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCa                                                                                         >  2:5326019/1‑93 (MQ=255)

     gATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCAcc                                                                                       >  1:4994883/1‑93 (MQ=255)

       tatTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCCACAGCATTCCTGCCGAGACGCTGTGCACCtt                                                                                     >  1:5363968/1‑93 (MQ=255)

        atTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTg                                                                                    >  1:3808840/1‑93 (MQ=255)

         ttCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGa                                                                                   <  2:7065047/93‑1 (MQ=255)

           ccGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGc                                                                                 >  2:3875834/1‑93 (MQ=255)

            cGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCa                                                                                >  2:5432094/1‑93 (MQ=255)

             gctgctGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCaa                                                                               >  1:7214113/1‑93 (MQ=255)

              ctgctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAAc                                                                              >  2:4057471/1‑93 (MQ=255)

              ctgctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAAc                                                                              <  1:6372719/93‑1 (MQ=255)

               tgctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACa                                                                             >  2:7307780/1‑93 (MQ=255)

               tgctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACa                                                                             >  1:6929912/1‑93 (MQ=255)

               tgctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACa                                                                             >  2:3075770/1‑93 (MQ=255)

                gctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAt                                                                            <  2:112682/93‑1 (MQ=255)

                gctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAt                                                                            <  2:6707262/93‑1 (MQ=255)

                gctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAt                                                                            >  2:1361895/1‑93 (MQ=255)

                 ctgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATg                                                                           <  1:4685426/93‑1 (MQ=255)

                  tgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAtnn                                                                          >  1:2371989/1‑91 (MQ=255)

                  tgGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATgg                                                                          >  1:3481523/1‑93 (MQ=255)

                   ggCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGa                                                                         <  1:3207394/93‑1 (MQ=255)

                    gCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAg                                                                        >  1:6028341/1‑93 (MQ=255)

                     cGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGt                                                                       <  2:452150/93‑1 (MQ=255)

                      gAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTg                                                                      >  1:1034294/1‑93 (MQ=255)

                      gAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTg                                                                      >  1:2558144/1‑93 (MQ=255)

                       aaaGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTgg                                                                     >  1:5831124/1‑93 (MQ=255)

                        aaGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACGTGGAGTGGc                                                                    <  1:6751671/93‑1 (MQ=255)

                         aGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGcc                                                                   <  2:6305425/93‑1 (MQ=255)

                         aGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGcc                                                                   >  1:5542829/1‑93 (MQ=255)

                          gCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCg                                                                  >  2:724091/1‑93 (MQ=255)

                            cTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCggg                                                                >  1:6804253/1‑93 (MQ=255)

                              ttACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTa                                                              <  2:1861648/93‑1 (MQ=255)

                                aCCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAAc                                                            >  1:7090724/1‑93 (MQ=255)

                                aCCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGCGGCCGGGGAAc                                                            >  2:760671/1‑93 (MQ=255)

                                 ccTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACg                                                           <  1:5703776/93‑1 (MQ=255)

                                 ccTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACg                                                           >  1:4514601/1‑93 (MQ=255)

                                  cTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGt                                                          <  2:871200/93‑1 (MQ=255)

                                  cTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGt                                                          >  2:2160775/1‑93 (MQ=255)

                                  cTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGt                                                          <  2:4270928/93‑1 (MQ=255)

                                   ttCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTa                                                         <  2:6912191/93‑1 (MQ=255)

                                   ttCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGGAACGTa                                                         >  1:4938437/1‑93 (MQ=255)

                                    tCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAc                                                        <  1:1842362/93‑1 (MQ=255)

                                     cAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAcg                                                       >  2:198/1‑93 (MQ=255)

                                      aaaaTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAcgc                                                      >  1:2070907/1‑93 (MQ=255)

                                      aaaaTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAcgc                                                      >  1:7305912/1‑93 (MQ=255)

                                      aaaaTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAcgc                                                      <  2:1619139/93‑1 (MQ=255)

                                         aTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGaa                                                   >  2:5604485/1‑93 (MQ=255)

                                         aTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGaa                                                   >  2:1916945/1‑93 (MQ=255)

                                         aTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGaa                                                   <  2:1224013/93‑1 (MQ=255)

                                          ttGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAAc                                                  >  2:3303960/1‑93 (MQ=255)

                                           tGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACt                                                 <  2:1693571/93‑1 (MQ=255)

                                           tGCCCGCCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCAGGGTAACGTACGCGAACt                                                 <  1:1321127/93‑1 (MQ=255)

                                            gCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTg                                                <  2:2587435/93‑1 (MQ=255)

                                            gCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTg                                                <  2:338689/93‑1 (MQ=255)

                                              ccGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGa                                              >  1:6180925/1‑93 (MQ=255)

                                              ccGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGa                                              >  1:3589419/1‑93 (MQ=255)

                                               cgtcgtCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGaa                                             >  2:4422044/1‑93 (MQ=255)

                                                gtcgtcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGaaa                                            >  1:940218/1‑93 (MQ=255)

                                                 tcgtcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGaaaa                                           <  1:1721171/93‑1 (MQ=255)

                                                   gtcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACg                                         >  1:6545623/1‑93 (MQ=255)

                                                    tcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGt                                        >  2:3643187/1‑93 (MQ=255)

                                                       ggggCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCAt                                     >  1:2956152/1‑93 (MQ=255)

                                                       ggggCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCAt                                     >  2:2972194/1‑93 (MQ=255)

                                                         ggCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTg                                   >  2:4851730/1‑93 (MQ=255)

                                                         ggCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTg                                   <  1:3857829/93‑1 (MQ=255)

                                                          gggcAATATCGACAGCATGCCTGCCGAGACGCTGTGCACCTTGAGCAACATGAAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGa                                  <  1:6578978/91‑1 (MQ=255)

                                                           cgcAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAg                                 <  2:4218921/93‑1 (MQ=255)

                                                            gcAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgc                                >  1:5906079/1‑93 (MQ=255)

                                                              aaTATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgcgc                              >  1:1557852/1‑93 (MQ=255)

                                                              aaTATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgcgc                              >  2:5741127/1‑93 (MQ=255)

                                                               atatCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgcgcg                             <  1:3979497/93‑1 (MQ=255)

                                                               atatCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgcgcg                             >  1:6335240/1‑93 (MQ=255)

                                                                tatCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAgcgcgc                            >  2:3859035/1‑93 (MQ=255)

                                                                 atCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGcgcgcg                           >  1:5751551/1‑93 (MQ=255)

                                                                  tCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCgg                          <  2:4134348/93‑1 (MQ=255)

                                                                    gACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTa                        >  1:5231045/1‑93 (MQ=255)

                                                                    gACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTa                        >  1:3507724/1‑93 (MQ=255)

                                                                    gACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTa                        <  2:1076678/93‑1 (MQ=255)

                                                                     aCAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAt                       <  2:3503019/93‑1 (MQ=255)

                                                                     aCAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAt                       >  1:5190976/1‑93 (MQ=255)

                                                                      cAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAtt                      <  2:2352134/93‑1 (MQ=255)

                                                                      cAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAtt                      >  2:3750190/1‑93 (MQ=255)

                                                                        gCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGc                    >  2:5532276/1‑93 (MQ=255)

                                                                         cATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCt                   <  2:5237676/93‑1 (MQ=255)

                                                                          aTTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTa                  >  2:2673083/1‑93 (MQ=255)

                                                                           ttCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTaa                 >  1:1441409/1‑93 (MQ=255)

                                                                           ttCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATGGCTaa                 <  2:4592419/93‑1 (MQ=255)

                                                                              cTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAacac              >  2:3012710/1‑93 (MQ=255)

                                                                                  cGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCgg          >  2:6631694/1‑93 (MQ=255)

                                                                                    agaCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTa        <  1:7359484/93‑1 (MQ=255)

                                                                                     gaCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTaa       <  2:4745081/93‑1 (MQ=255)

                                                                                     gaCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTaa       <  2:4469871/93‑1 (MQ=255)

                                                                                     gaCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTaa       >  1:2843212/1‑93 (MQ=255)

                                                                                     gaCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTaa       <  1:4227159/93‑1 (MQ=255)

                                                                                     gaCGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTaa       <  2:6066438/93‑1 (MQ=255)

                                                                                      aCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAAc      >  2:95233/1‑93 (MQ=255)

                                                                                        gCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGt    >  2:2817298/1‑93 (MQ=255)

                                                                                        gCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGt    >  2:2095463/1‑93 (MQ=255)

                                                                                        gCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGt    <  1:1062130/93‑1 (MQ=255)

                                                                                        gCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGAGGTAACGt    <  1:5120961/93‑1 (MQ=255)

                                                                                         cTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGtg   <  1:788184/93‑1 (MQ=255)

CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGC  >  NC_000913/2854027‑2854209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTG  >  NC_000913/2854026‑2854211

CCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGT                                                                                              >  SRR4302542.3493039/1‑94 (MQ=60)

CCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGT                                                                                              >  SRR4302542.3493038/1‑94 (MQ=60)

 CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             >  SRR4302542.4233933/1‑94 (MQ=60)

 CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             <  SRR4302542.6527565/94‑1 (MQ=60)

 CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             <  SRR4302542.1422134/94‑1 (MQ=60)

 CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             >  SRR4302542.5130074/1‑94 (MQ=60)

 CGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             <  SRR4302542.6268199/94‑1 (MQ=60)

 CGGAAGAGATTCCGCTGCGGGCGAAAGCCCTTCCCTTCAAAATTGCCCGTCATCGGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTG                                                                                             <  SRR4302542.6527566/94‑1 (MQ=60)

  GGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGC                                                                                            >  SRR4302542.4069073/1‑94 (MQ=60)

    AAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCAC                                                                                          <  SRR4302542.7302737/94‑1 (MQ=60)

    AAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCAC                                                                                          <  SRR4302542.4999493/94‑1 (MQ=60)

    AAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCAC                                                                                          <  SRR4302542.1687198/94‑1 (MQ=60)

     AGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACC                                                                                         >  SRR4302542.5043409/1‑94 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       >  SRR4302542.1997546/1‑94 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       >  SRR4302542.783696/1‑94 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       <  SRR4302542.5873864/94‑1 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       >  SRR4302542.4615159/1‑94 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       >  SRR4302542.3537531/1‑94 (MQ=60)

       ATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCCACAGCATTCCTGCCGAGACGCTGTGCACCTT                                                                                       >  SRR4302542.5416100/1‑94 (MQ=60)

        TATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTG                                                                                      >  SRR4302542.3846525/1‑94 (MQ=60)

        TATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTG                                                                                      >  SRR4302542.6847817/1‑94 (MQ=60)

            CCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCA                                                                                  <  SRR4302542.2553464/94‑1 (MQ=60)

             CGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAA                                                                                 >  SRR4302542.7284899/1‑94 (MQ=60)

              GCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAAC                                                                                <  SRR4302542.7377779/94‑1 (MQ=60)

               CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACA                                                                               <  SRR4302542.6435475/94‑1 (MQ=60)

               CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACA                                                                               >  SRR4302542.704726/1‑94 (MQ=60)

               CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACA                                                                               >  SRR4302542.6997949/1‑94 (MQ=60)

               CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACA                                                                               >  SRR4302542.5239209/1‑94 (MQ=60)

                TGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAT                                                                              <  SRR4302542.6410710/94‑1 (MQ=60)

                TGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACAT                                                                              <  SRR4302542.415661/94‑1 (MQ=60)

                 GCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATG                                                                             <  SRR4302542.1341604/94‑1 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATNN                                                                            >  SRR4302542.2392893/1‑94 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGG                                                                            <  SRR4302542.7425524/94‑1 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGG                                                                            >  SRR4302542.3514101/1‑94 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGG                                                                            >  SRR4302542.1970485/1‑94 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGG                                                                            <  SRR4302542.6804656/94‑1 (MQ=60)

                  CTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGG                                                                            <  SRR4302542.4731192/94‑1 (MQ=60)

                    GGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAG                                                                          <  SRR4302542.4521985/94‑1 (MQ=60)

                    GGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAG                                                                          <  SRR4302542.3237773/94‑1 (MQ=60)

                    GGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAG                                                                          <  SRR4302542.7361206/94‑1 (MQ=60)

                    GGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAG                                                                          >  SRR4302542.6086713/1‑94 (MQ=60)

                    GGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAG                                                                          >  SRR4302542.6626170/1‑94 (MQ=60)

                      NNAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTG                                                                        <  SRR4302542.3248344/94‑1 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTG                                                                        >  SRR4302542.59330/1‑94 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTG                                                                        <  SRR4302542.1051121/94‑1 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTG                                                                        <  SRR4302542.1313330/94‑1 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTG                                                                        >  SRR4302542.1043183/1‑94 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTG                                                                        >  SRR4302542.3578129/1‑94 (MQ=60)

                      CGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTG                                                                        >  SRR4302542.2582942/1‑94 (MQ=60)

                       GAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGG                                                                       >  SRR4302542.5887706/1‑94 (MQ=60)

                       GAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGG                                                                       >  SRR4302542.6862299/1‑94 (MQ=60)

                         AAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACGTGGAGTGGCC                                                                     <  SRR4302542.6818089/94‑1 (MQ=60)

                         AAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCC                                                                     >  SRR4302542.5596704/1‑94 (MQ=60)

                          AGCCTTCACCTGCAAAATTGCCCGACGTCTTGGGCTCAATATCAACAGCATTCCTGCCGAGACGCTTTGCACCTTAAGCAACATGGAGTGTCCG                                                                    <  SRR4302542.5013484/94‑1 (MQ=44)

                           GCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGG                                                                   >  SRR4302542.5896453/1‑94 (MQ=60)

                            CCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGG                                                                  >  SRR4302542.6871155/1‑94 (MQ=60)

                            CCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGG                                                                  >  SRR4302542.4940007/1‑94 (MQ=60)

                             CTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGT                                                                 >  SRR4302542.3873658/1‑94 (MQ=60)

                             CTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGT                                                                 >  SRR4302542.536910/1‑94 (MQ=60)

                              TTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTA                                                                >  SRR4302542.5653834/1‑94 (MQ=60)

                               TTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAA                                                               >  SRR4302542.5438499/1‑94 (MQ=60)

                                TACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGCCGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAAC                                                              >  SRR4302542.6126589/1‑94 (MQ=60)

                                TACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAAC                                                              >  SRR4302542.7160291/1‑94 (MQ=60)

                                 ACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACG                                                             >  SRR4302542.696108/1‑94 (MQ=60)

                                 ACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACG                                                             <  SRR4302542.1208564/94‑1 (MQ=60)

                                 ACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACG                                                             >  SRR4302542.4558841/1‑94 (MQ=60)

                                 ACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACG                                                             >  SRR4302542.5270037/1‑94 (MQ=60)

                                  CCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGT                                                            <  SRR4302542.5759143/94‑1 (MQ=60)

                                  CCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGGAACGT                                                            >  SRR4302542.3567749/1‑94 (MQ=60)

                                   CTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGGAACGTA                                                           >  SRR4302542.4986437/1‑94 (MQ=60)

                                    TTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAC                                                          <  SRR4302542.5079213/94‑1 (MQ=60)

                                    TTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTAC                                                          <  SRR4302542.384990/94‑1 (MQ=60)

                                     TCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACG                                                         <  SRR4302542.1858307/94‑1 (MQ=60)

                                     TCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACG                                                         <  SRR4302542.4004240/94‑1 (MQ=60)

                                     TCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACG                                                         <  SRR4302542.6593676/94‑1 (MQ=60)

                                      CAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGC                                                        >  SRR4302542.7377480/1‑94 (MQ=60)

                                      CAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGC                                                        >  SRR4302542.2088805/1‑94 (MQ=60)

                                       AAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCG                                                       >  SRR4302542.3157266/1‑94 (MQ=60)

                                        AAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGA                                                      >  SRR4302542.2915242/1‑94 (MQ=60)

                                            TGCCCGCCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCAGGGTAACGTACGCGAACTG                                                  <  SRR4302542.1332503/94‑1 (MQ=60)

                                             GCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGG                                                 >  SRR4302542.6797077/1‑94 (MQ=60)

                                              CCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGA                                                >  SRR4302542.3622894/1‑94 (MQ=60)

                                              CCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGA                                                >  SRR4302542.6240884/1‑94 (MQ=60)

                                                CGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAA                                              >  SRR4302542.948310/1‑94 (MQ=60)

                                                CGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGCACGCGAACTGGAAA                                              >  SRR4302542.6112042/1‑94 (MQ=60)

                                                 GTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAA                                             >  SRR4302542.3174322/1‑94 (MQ=60)

                                                  TCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAAC                                            <  SRR4302542.1736116/94‑1 (MQ=60)

                                                  TCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAAC                                            <  SRR4302542.4884554/94‑1 (MQ=60)

                                                   CGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACG                                           >  SRR4302542.3824053/1‑94 (MQ=60)

                                                   CGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACG                                           >  SRR4302542.6610169/1‑94 (MQ=60)

                                                   CGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACG                                           >  SRR4302542.1812727/1‑94 (MQ=60)

                                                    GTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGT                                          >  SRR4302542.5798056/1‑94 (MQ=60)

                                                     TCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTC                                         <  SRR4302542.3387509/94‑1 (MQ=60)

                                                       TGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCAT                                       >  SRR4302542.2984456/1‑94 (MQ=60)

                                                         GGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTG                                     <  SRR4302542.3801416/94‑1 (MQ=60)

                                                          GGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGA                                    <  SRR4302542.3895932/94‑1 (MQ=60)

                                                           GGGCAATATCGACAGCATGCCTGCCGAGACGCTGTGCACCTTGAGCAACATGAAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAG                                   <  SRR4302542.6643806/94‑1 (MQ=60)

                                                           GCGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAG                                   >  SRR4302542.2681452/1‑94 (MQ=60)

                                                           GCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAG                                   <  SRR4302542.1532618/94‑1 (MQ=60)

                                                            CGCAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGC                                  >  SRR4302542.5963317/1‑94 (MQ=60)

                                                              CAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGC                                >  SRR4302542.1571297/1‑94 (MQ=60)

                                                              CAATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGC                                >  SRR4302542.656704/1‑94 (MQ=60)

                                                               AATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCG                               >  SRR4302542.6397659/1‑94 (MQ=60)

                                                               AATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCG                               >  SRR4302542.2380484/1‑94 (MQ=60)

                                                               AATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCG                               >  SRR4302542.5438940/1‑94 (MQ=60)

                                                                ATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGC                              <  SRR4302542.4018758/94‑1 (MQ=60)

                                                                ATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGC                              >  SRR4302542.3911017/1‑94 (MQ=60)

                                                                ATATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGC                              <  SRR4302542.1343537/94‑1 (MQ=60)

                                                                 TATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCG                             >  SRR4302542.5807381/1‑94 (MQ=60)

                                                                  ATCGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGG                            >  SRR4302542.7225727/1‑94 (MQ=60)

                                                                    CGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTA                          >  SRR4302542.3540526/1‑94 (MQ=60)

                                                                    CGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTA                          >  SRR4302542.5281914/1‑94 (MQ=60)

                                                                    CGACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTA                          >  SRR4302542.5132763/1‑94 (MQ=60)

                                                                     GACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAT                         <  SRR4302542.2150506/94‑1 (MQ=60)

                                                                     GACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAT                         >  SRR4302542.1818029/1‑94 (MQ=60)

                                                                     GACAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTAT                         >  SRR4302542.5241406/1‑94 (MQ=60)

                                                                     GACAGCATTCCTACCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGGAT                         >  SRR4302542.5706353/1‑94 (MQ=60)

                                                                       aacCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGGCGGGGAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTG                       <  SRR4302542.2891121/91‑1 (MQ=60)

                                                                       CAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTG                       <  SRR4302542.6418552/94‑1 (MQ=60)

                                                                       CAGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTG                       >  SRR4302542.1700136/1‑94 (MQ=60)

                                                                        AGCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGC                      >  SRR4302542.6616019/1‑94 (MQ=60)

                                                                         GCATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCT                     >  SRR4302542.6057103/1‑94 (MQ=60)

                                                                         GCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCT                     <  SRR4302542.5284802/94‑1 (MQ=60)

                                                                          CATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTA                    >  SRR4302542.471681/1‑94 (MQ=60)

                                                                           ATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTNTNGCTAA                   >  SRR4302542.6926462/1‑94 (MQ=60)

                                                                           ATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAA                   >  SRR4302542.5228424/1‑94 (MQ=60)

                                                                           ATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAA                   >  SRR4302542.1453856/1‑94 (MQ=60)

                                                                           ATTCCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAA                   >  SRR4302542.3533280/1‑94 (MQ=60)

                                                                              CCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACAC                >  SRR4302542.45164/1‑94 (MQ=60)

                                                                              CCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACAC                >  SRR4302542.5759492/1‑94 (MQ=60)

                                                                              CCTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACAC                <  SRR4302542.6073088/94‑1 (MQ=60)

                                                                              CCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACAC                >  SRR4302542.942221/1‑94 (MQ=60)

                                                                               CTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACG               >  SRR4302542.6394347/1‑94 (MQ=60)

                                                                               CTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACG               >  SRR4302542.7283078/1‑94 (MQ=60)

                                                                               CTGCCGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACG               <  SRR4302542.1564139/94‑1 (MQ=60)

                                                                               CTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACG               >  SRR4302542.7011906/1‑94 (MQ=60)

                                                                                   CGAGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGT           >  SRR4302542.2741777/1‑94 (MQ=60)

                                                                                     AGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAA         >  SRR4302542.6372716/1‑94 (MQ=60)

                                                                                     AGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAA         <  SRR4302542.7431576/94‑1 (MQ=60)

                                                                                     AGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAA         <  SRR4302542.7431575/94‑1 (MQ=60)

                                                                                     AGACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAA         >  SRR4302542.2870531/1‑94 (MQ=60)

                                                                                     AGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAA         >  SRR4302542.845910/1‑94 (MQ=60)

                                                                                      GACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAAC        <  SRR4302542.4268802/94‑1 (MQ=60)

                                                                                       ACGCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACG       >  SRR4302542.2498918/1‑94 (MQ=60)

                                                                                         GCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTG     <  SRR4302542.1071260/94‑1 (MQ=60)

                                                                                         GCTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGAGGTAACGTG     <  SRR4302542.5170690/94‑1 (MQ=60)

                                                                                         GCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACCGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTG     <  SRR4302542.5878298/94‑1 (MQ=60)

                                                                                          CTGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGC    <  SRR4302542.794974/94‑1 (MQ=60)

                                                                                           TGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCT   <  SRR4302542.7084292/94‑1 (MQ=60)

                                                                                           TGTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCT   <  SRR4302542.7084291/94‑1 (MQ=60)

                                                                                            GTGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTG  <  SRR4302542.3179161/94‑1 (MQ=60)

CCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTG  >  NC_000913/2854026‑2854211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`