Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,284	T→C	V390A (GTA→GCA)	mutS →	MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,284	0	T	C	83.7%	645.1 / 115.1	288	V390A (GTA→GCA)	mutS	MutS
Reads supporting (aligned to +/- strand): ref base T (22/25); new base C (111/130); total (133/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 291 total aligned reads displayed.

GCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCG  >  NC_000913/2856193‑2856376

gCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTa                                                                                             <  1:3491540/93‑1 (MQ=255)

 cTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCAc                                                                                            >  1:2989396/1‑93 (MQ=255)

   cGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAg                                                                                          >  1:3764515/1‑93 (MQ=255)

    gCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAgg                                                                                         <  2:3647525/93‑1 (MQ=255)

       cACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGgcgc                                                                                      >  2:2834515/1‑93 (MQ=255)

       cACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGgcgc                                                                                      >  2:7440810/1‑93 (MQ=255)

       cACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGgcgc                                                                                      >  1:640699/1‑93 (MQ=255)

       cACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGgcgc                                                                                      <  2:5066747/93‑1 (MQ=255)

         cgcgATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTa                                                                                    >  2:1520487/1‑93 (MQ=255)

            gATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGt                                                                                 >  2:2493392/1‑93 (MQ=255)

             aTCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTg                                                                                >  1:5880600/1‑93 (MQ=255)

             aTCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTg                                                                                <  2:6648417/93‑1 (MQ=255)

                tGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTgaga                                                                             >  1:4692432/1‑93 (MQ=255)

                tGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTgaga                                                                             >  2:531536/1‑93 (MQ=255)

                 ggCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGagaa                                                                            >  2:4119615/1‑93 (MQ=255)

                 ggCCCGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa                                                                            >  2:1154723/1‑93 (MQ=255)

                  gCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGagaag                                                                           <  1:534266/93‑1 (MQ=255)

                   cccGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGagaaga                                                                          >  2:1875969/1‑93 (MQ=255)

                   cccGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGagaaga                                                                          >  2:6858433/1‑93 (MQ=255)

                   cccGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga                                                                          <  2:6146062/93‑1 (MQ=255)

                    ccGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAt                                                                         <  2:1088762/93‑1 (MQ=255)

                    ccGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAt                                                                         >  2:5408842/1‑93 (MQ=255)

                     cGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATg                                                                        <  2:6103757/93‑1 (MQ=255)

                     cGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATg                                                                        <  2:500160/93‑1 (MQ=255)

                          gcgcCCCGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGa                                                                   >  2:4595724/1‑93 (MQ=255)

                           cgcCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAg                                                                  <  2:4520414/93‑1 (MQ=255)

                           cgcCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAg                                                                  <  2:6506398/93‑1 (MQ=255)

                            gcCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGt                                                                 <  1:6162801/93‑1 (MQ=255)

                             ccACGCTTTCCAGCAACTGCTGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGtt                                                                <  1:7467948/93‑1 (MQ=255)

                              cACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGttt                                                               <  2:342175/93‑1 (MQ=255)

                              cACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGttt                                                               >  2:3357532/1‑93 (MQ=255)

                                cGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGc                                                             >  1:4332734/1‑93 (MQ=255)

                                cGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGc                                                             <  1:2846684/93‑1 (MQ=255)

                                 gCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGcc                                                            <  2:7066725/93‑1 (MQ=255)

                                 gCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGcc                                                            <  1:4506390/93‑1 (MQ=255)

                                 gCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGcc                                                            <  1:3315515/93‑1 (MQ=255)

                                   tttCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGa                                                          >  1:3665867/1‑93 (MQ=255)

                                      ccAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt                                                       <  2:2201988/93‑1 (MQ=255)

                                      ccAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt                                                       <  1:3830527/93‑1 (MQ=255)

                                      ccAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt                                                       >  1:6724350/1‑93 (MQ=255)

                                       cAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg                                                      <  1:6084485/93‑1 (MQ=255)

                                        aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgc                                                     <  2:6462441/93‑1 (MQ=255)

                                        aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgc                                                     <  2:5216526/93‑1 (MQ=255)

                                           aaCTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGcgcg                                                  >  2:1936692/1‑93 (MQ=255)

                                            aCTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGa                                                 >  1:2665728/1‑93 (MQ=255)

                                             cTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGAt                                                <  1:5448728/93‑1 (MQ=255)

                                              tGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc                                               <  2:1318813/93‑1 (MQ=255)

                                               gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATct                                              >  1:772378/1‑93 (MQ=255)

                                               gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATct                                              <  1:5715096/93‑1 (MQ=255)

                                               gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATct                                              <  1:1280072/93‑1 (MQ=255)

                                               gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATct                                              >  2:6721179/1‑93 (MQ=255)

                                                 cGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgc                                            <  2:7424185/93‑1 (MQ=255)

                                                 cGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgc                                            <  1:1459363/93‑1 (MQ=255)

                                                  ggAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgct                                           <  2:1758587/93‑1 (MQ=255)

                                                  ggAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgct                                           >  1:1017783/1‑93 (MQ=255)

                                                  ggAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgct                                           <  2:4920683/93‑1 (MQ=255)

                                                   gAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgctg                                          >  2:6135171/1‑93 (MQ=255)

                                                   gAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgctg                                          >  2:3158420/1‑93 (MQ=255)

                                                     gCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGga                                        >  1:2270170/1‑93 (MQ=255)

                                                       tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagc                                      >  2:3390641/1‑93 (MQ=255)

                                                       tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagc                                      >  1:2221069/1‑93 (MQ=255)

                                                       tgcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagc                                      >  1:1661499/1‑93 (MQ=255)

                                                         cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcga                                    <  2:5121999/93‑1 (MQ=255)

                                                         cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcga                                    <  2:819278/93‑1 (MQ=255)

                                                         cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcga                                    <  2:1020979/93‑1 (MQ=255)

                                                         cgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcga                                    <  1:5519428/93‑1 (MQ=255)

                                                            gcgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa                                 >  2:348886/1‑93 (MQ=255)

                                                              gcAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat                               <  1:2856495/93‑1 (MQ=255)

                                                               cAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatc                              >  2:3748103/1‑93 (MQ=255)

                                                               cAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatc                              <  1:1522485/93‑1 (MQ=255)

                                                                aGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatca                             <  1:4848416/93‑1 (MQ=255)

                                                                aGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatca                             >  1:958829/1‑93 (MQ=255)

                                                                 gTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcat                            <  1:391640/93‑1 (MQ=255)

                                                                  ttAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc                           <  2:6004764/93‑1 (MQ=255)

                                                                  ttAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc                           >  1:6965267/1‑93 (MQ=255)

                                                                  ttAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc                           >  1:6338382/1‑93 (MQ=255)

                                                                  ttAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc                           <  1:554338/93‑1 (MQ=255)

                                                                   tAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCg                          >  1:2001023/1‑93 (MQ=255)

                                                                    aGAAACTGTCGATAGTGCGCCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa                         <  1:4186864/93‑1 (MQ=255)

                                                                     gAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGac                        <  1:2546614/93‑1 (MQ=255)

                                                                     gAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGac                        <  2:5522412/93‑1 (MQ=255)

                                                                     gAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGac                        <  2:239097/93‑1 (MQ=255)

                                                                      aaaCTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGaca                       <  2:4458713/93‑1 (MQ=255)

                                                                         cTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGacacac                    <  2:2703278/93‑1 (MQ=255)

                                                                         cTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGacacac                    <  2:226990/93‑1 (MQ=255)

                                                                          tGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAcc                   >  2:1595803/1‑93 (MQ=255)

                                                                           gTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAccg                  >  1:3599611/1‑93 (MQ=255)

                                                                            tCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAccgc                 >  2:1672469/1‑93 (MQ=255)

                                                                            tCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAccgc                 <  1:1246447/93‑1 (MQ=255)

                                                                             cGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAccgcc                <  1:7157445/93‑1 (MQ=255)

                                                                              gATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAccgccg               >  1:4904059/1‑93 (MQ=255)

                                                                               aTAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCgg              <  2:4207125/93‑1 (MQ=255)

                                                                               aTAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCgg              >  1:508937/1‑93 (MQ=255)

                                                                               aTAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCgg              >  1:137569/1‑93 (MQ=255)

                                                                                tAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGt             <  2:567977/93‑1 (MQ=255)

                                                                                  gTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGc           >  1:5010038/1‑93 (MQ=255)

                                                                                  gTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGc           <  1:3153396/93‑1 (MQ=255)

                                                                                     cACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTgg        <  1:5129275/93‑1 (MQ=255)

                                                                                       ccGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTa      <  1:2334122/93‑1 (MQ=255)

                                                                                         ggTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTAcg    >  2:7140887/1‑93 (MQ=255)

GCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCG  >  NC_000913/2856193‑2856376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCGA  >  NC_000913/2856191‑2856377

CTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGC                                                                                               >  SRR4302542.4276356/1‑94 (MQ=60)

CTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGC                                                                                               <  SRR4302542.7596816/94‑1 (MQ=60)

  GCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCAC                                                                                             >  SRR4302542.3018010/1‑94 (MQ=60)

  GCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTAC                                                                                             <  SRR4302542.3524191/94‑1 (MQ=60)

   CTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACA                                                                                            >  SRR4302542.5528639/1‑94 (MQ=60)

    TCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAG                                                                                           >  SRR4302542.3801750/1‑94 (MQ=60)

     CGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGG                                                                                          <  SRR4302542.6464531/94‑1 (MQ=60)

     CGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGG                                                                                          <  SRR4302542.5121998/94‑1 (MQ=60)

       CCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCG                                                                                        >  SRR4302542.3719467/1‑94 (MQ=60)

        CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGC                                                                                       >  SRR4302542.646211/1‑94 (MQ=60)

        CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGC                                                                                       >  SRR4302542.7583070/1‑94 (MQ=60)

          ACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTA                                                                                     <  SRR4302542.2275477/94‑1 (MQ=60)

             CGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGT                                                                                  >  SRR4302542.2777979/1‑94 (MQ=60)

              GATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTG                                                                                 >  SRR4302542.5937612/1‑94 (MQ=60)

                TCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAG                                                                               <  SRR4302542.5512519/94‑1 (MQ=60)

                TCTGGCCCGTATGCGCCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAG                                                                               <  SRR4302542.3688147/94‑1 (MQ=60)

                 CTGNCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGA                                                                              <  SRR4302542.5592634/94‑1 (MQ=60)

                 CTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGA                                                                              >  SRR4302542.4738251/1‑94 (MQ=60)

                 CTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGA                                                                              >  SRR4302542.4411353/1‑94 (MQ=60)

                  TGGCCCGTATGCGCCACGCTTTCCAGCAACTTCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAA                                                                             <  SRR4302542.2880717/94‑1 (MQ=60)

                   GGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAG                                                                            <  SRR4302542.2027588/94‑1 (MQ=60)

                    GCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGA                                                                           <  SRR4302542.219617/94‑1 (MQ=60)

                    GCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGA                                                                           >  SRR4302542.1270873/1‑94 (MQ=60)

                    GCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGA                                                                           <  SRR4302542.538881/94‑1 (MQ=60)

                     CCCGTATGCGCTACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAT                                                                          <  SRR4302542.7509618/94‑1 (MQ=60)

                     CCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAT                                                                          <  SRR4302542.4072233/94‑1 (MQ=60)

                     CCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAT                                                                          >  SRR4302542.5098265/1‑94 (MQ=60)

                     CCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGAT                                                                          >  SRR4302542.1878900/1‑94 (MQ=60)

                      CCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATG                                                                         <  SRR4302542.6755568/94‑1 (MQ=60)

                       CGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGG                                                                        >  SRR4302542.1557985/1‑94 (MQ=60)

                       CGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGG                                                                        <  SRR4302542.6356437/94‑1 (MQ=60)

                        GTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGG                                                                       <  SRR4302542.1425159/94‑1 (MQ=60)

                        GTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGG                                                                       <  SRR4302542.763792/94‑1 (MQ=60)

                            GCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAG                                                                   <  SRR4302542.2345377/94‑1 (MQ=60)

                              GCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTT                                                                 >  SRR4302542.5596573/1‑94 (MQ=60)

                              GCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTT                                                                 <  SRR4302542.6222500/94‑1 (MQ=60)

                              ACCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTT                                                                 <  SRR4302542.6222497/94‑1 (MQ=60)

                               CCACGCTTTCCAGCAACTGCTGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTT                                                                <  SRR4302542.7541120/94‑1 (MQ=60)

                               CCACGCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTT                                                                >  SRR4302542.5990069/1‑94 (MQ=60)

                                CACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTG                                                               <  SRR4302542.4797774/94‑1 (MQ=60)

                                CACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTG                                                               >  SRR4302542.7180729/1‑94 (MQ=60)

                                 ACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGC                                                              <  SRR4302542.2980509/94‑1 (MQ=60)

                                 ACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGC                                                              >  SRR4302542.4375318/1‑94 (MQ=60)

                                  CGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCC                                                             <  SRR4302542.2874043/94‑1 (MQ=60)

                                   GCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCG                                                            <  SRR4302542.4550562/94‑1 (MQ=60)

                                   GCTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCG                                                            <  SRR4302542.3346760/94‑1 (MQ=60)

                                    CTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGA                                                           <  SRR4302542.3522380/94‑1 (MQ=60)

                                    CTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGA                                                           >  SRR4302542.3700754/1‑94 (MQ=60)

                                    CTTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGA                                                           >  SRR4302542.1641768/1‑94 (MQ=60)

                                     TTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAG                                                          >  SRR4302542.5805723/1‑94 (MQ=60)

                                     TTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAG                                                          >  SRR4302542.5158800/1‑94 (MQ=60)

                                     TTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAG                                                          >  SRR4302542.4936917/1‑94 (MQ=60)

                                     TTTCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAG                                                          >  SRR4302542.2314514/1‑94 (MQ=60)

                                      TTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGC                                                         <  SRR4302542.2457035/94‑1 (MQ=60)

                                       TCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCT                                                        >  SRR4302542.4980878/1‑94 (MQ=60)

                                       TCCAGCAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCT                                                        >  SRR4302542.6790560/1‑94 (MQ=60)

                                        CCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTG                                                       <  SRR4302542.3868415/94‑1 (MQ=60)

                                         CAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGC                                                      <  SRR4302542.6143398/94‑1 (MQ=60)

                                         CAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGC                                                      <  SRR4302542.5019924/94‑1 (MQ=60)

                                          AGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCG                                                     <  SRR4302542.1995650/94‑1 (MQ=60)

                                           GCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGC                                                    <  SRR4302542.1497117/94‑1 (MQ=60)

                                           GCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGC                                                    >  SRR4302542.3259644/1‑94 (MQ=60)

                                           GCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGC                                                    >  SRR4302542.6451875/1‑94 (MQ=60)

                                            CAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCG                                                   >  SRR4302542.2816003/1‑94 (MQ=60)

                                            CAACGGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCG                                                   <  SRR4302542.5249200/94‑1 (MQ=60)

                                             AACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGA                                                  >  SRR4302542.3408405/1‑94 (MQ=60)

                                             AACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGA                                                  >  SRR4302542.2691465/1‑94 (MQ=60)

                                              ACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGAT                                                 <  SRR4302542.5146862/94‑1 (MQ=60)

                                               tgGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATC                                                <  SRR4302542.2449341/92‑1 (MQ=60)

                                               CTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATC                                                <  SRR4302542.5501666/94‑1 (MQ=60)

                                                TGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCT                                               <  SRR4302542.5281477/94‑1 (MQ=60)

                                                TGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCT                                               <  SRR4302542.6887013/94‑1 (MQ=60)

                                                TGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCT                                               >  SRR4302542.3055241/1‑94 (MQ=60)

                                                GGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCT                                               >  SRR4302542.779053/1‑94 (MQ=60)

                                                 GCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTG                                              <  SRR4302542.5770564/94‑1 (MQ=60)

                                                 GCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTG                                              <  SRR4302542.1291094/94‑1 (MQ=60)

                                                   CGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCT                                            <  SRR4302542.1471962/94‑1 (MQ=60)

                                                   CGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCT                                            >  SRR4302542.1026548/1‑94 (MQ=60)

                                                    GGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTG                                           >  SRR4302542.1352793/1‑94 (MQ=60)

                                                     GAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGG                                          >  SRR4302542.2572362/1‑94 (MQ=60)

                                                      AGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGA                                         >  SRR4302542.6208016/1‑94 (MQ=60)

                                                      AGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGA                                         >  SRR4302542.4066257/1‑94 (MQ=60)

                                                      AGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGA                                         >  SRR4302542.2289695/1‑94 (MQ=60)

                                                      AGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGA                                         >  SRR4302542.3057757/1‑94 (MQ=60)

                                                       GCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAG                                        >  SRR4302542.383471/1‑94 (MQ=60)

                                                        CTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGC                                       <  SRR4302542.4695529/94‑1 (MQ=60)

                                                        CTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGC                                       <  SRR4302542.571277/94‑1 (MQ=60)

                                                        CTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGC                                       >  SRR4302542.1675850/1‑94 (MQ=60)

                                                        CTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGC                                       >  SRR4302542.2240173/1‑94 (MQ=60)

                                                          GCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGA                                     <  SRR4302542.6357822/94‑1 (MQ=60)

                                                           CGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAG                                    <  SRR4302542.287383/94‑1 (MQ=60)

                                                           CGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAG                                    <  SRR4302542.5573069/94‑1 (MQ=60)

                                                            GTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGC                                   <  SRR4302542.452709/94‑1 (MQ=60)

                                                            GTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGC                                   >  SRR4302542.2274434/1‑94 (MQ=60)

                                                            GTGCGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGC                                   >  SRR4302542.4941615/1‑94 (MQ=60)

                                                              GCGCAGTTAGAACCTGTCGATAGTGCTCCGGTACAGGCGCTACGTGAGAAGAGGGGCGAGTTTGCAGACCTGCGCGATCTGCTGGAGgaggcct                                 <  SRR4302542.3127082/94‑8 (MQ=60)

                                                               CGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAAT                                <  SRR4302542.5923630/94‑1 (MQ=60)

                                                               CGCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAAT                                >  SRR4302542.3535599/1‑94 (MQ=60)

                                                                GCAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATC                               <  SRR4302542.2883945/94‑1 (MQ=60)

                                                                 CAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCA                              <  SRR4302542.1535639/94‑1 (MQ=60)

                                                                 CAGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCA                              >  SRR4302542.967064/1‑94 (MQ=60)

                                                                  AGTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCAT                             <  SRR4302542.4895628/94‑1 (MQ=60)

                                                                   GTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATC                            >  SRR4302542.7033701/1‑94 (MQ=60)

                                                                   GTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATC                            <  SRR4302542.395024/94‑1 (MQ=60)

                                                                   GTTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATC                            >  SRR4302542.6400827/1‑94 (MQ=60)

                                                                    TTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCG                           >  SRR4302542.2018300/1‑94 (MQ=60)

                                                                    TTAGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCG                           <  SRR4302542.559122/94‑1 (MQ=60)

                                                                      AGAAACTGTCGATAGTGCGCCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGAC                         <  SRR4302542.4228154/94‑1 (MQ=60)

                                                                      AGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGAC                         <  SRR4302542.4353651/94‑1 (MQ=60)

                                                                      AGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGAC                         <  SRR4302542.785997/94‑1 (MQ=60)

                                                                      AGAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGAC                         <  SRR4302542.4798819/94‑1 (MQ=60)

                                                                       GAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACA                        <  SRR4302542.1522000/94‑1 (MQ=60)

                                                                       GAAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACA                        <  SRR4302542.2571293/94‑1 (MQ=60)

                                                                        AAACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACAC                       >  SRR4302542.99404/1‑94 (MQ=60)

                                                                         AACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACA                      >  SRR4302542.3775489/1‑94 (MQ=60)

                                                                          ACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC                     >  SRR4302542.1420738/1‑94 (MQ=60)

                                                                          ACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC                     <  SRR4302542.983939/94‑1 (MQ=60)

                                                                          ACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC                     >  SRR4302542.1344255/1‑94 (MQ=60)

                                                                          ACTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC                     <  SRR4302542.6478491/94‑1 (MQ=60)

                                                                           CTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACC                    <  SRR4302542.1159763/94‑1 (MQ=60)

                                                                           CTGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACC                    >  SRR4302542.3238893/1‑94 (MQ=60)

                                                                            TGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCG                   <  SRR4302542.598097/94‑1 (MQ=60)

                                                                            TGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCG                   <  SRR4302542.7024125/94‑1 (MQ=60)

                                                                            TGTCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCG                   >  SRR4302542.3633164/1‑94 (MQ=60)

                                                                              TCGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCC                 <  SRR4302542.1257162/94‑1 (MQ=60)

                                                                               CGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCG                <  SRR4302542.7227661/94‑1 (MQ=60)

                                                                               CGATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCG                >  SRR4302542.4951761/1‑94 (MQ=60)

                                                                                GATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGG               >  SRR4302542.78359/1‑94 (MQ=60)

                                                                                GATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGG               >  SRR4302542.138717/1‑94 (MQ=60)

                                                                                GATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGG               >  SRR4302542.513355/1‑94 (MQ=60)

                                                                                GATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGG               >  SRR4302542.7267863/1‑94 (MQ=60)

                                                                                 ATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGT              <  SRR4302542.391031/94‑1 (MQ=60)

                                                                                 ATAGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGT              <  SRR4302542.4720759/94‑1 (MQ=60)

                                                                                  TAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTG             <  SRR4302542.2888546/94‑1 (MQ=60)

                                                                                   AGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGC            >  SRR4302542.5058700/1‑94 (MQ=60)

                                                                                   AGTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGC            >  SRR4302542.3849092/1‑94 (MQ=60)

                                                                                    GTGCACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCT           <  SRR4302542.3183337/94‑1 (MQ=60)

                                                                                       CACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGT        >  SRR4302542.3645530/1‑94 (MQ=60)

                                                                                       CACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGT        <  SRR4302542.5179094/94‑1 (MQ=60)

                                                                                        ACCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTA       >  SRR4302542.567118/1‑94 (MQ=60)

                                                                                         CCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTAC      <  SRR4302542.1284824/94‑1 (MQ=60)

                                                                                         CCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTAC      <  SRR4302542.2354116/94‑1 (MQ=60)

                                                                                         CCGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTAC      <  SRR4302542.5439033/94‑1 (MQ=60)

                                                                                          CGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACG     <  SRR4302542.427311/94‑1 (MQ=60)

                                                                                          CGGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACG     >  SRR4302542.6495389/1‑94 (MQ=60)

                                                                                           GGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGC    >  SRR4302542.1578441/1‑94 (MQ=60)

                                                                                           GGCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGC    <  SRR4302542.3977855/94‑1 (MQ=60)

                                                                                            GTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCG   >  SRR4302542.3539223/1‑94 (MQ=60)

                                                                                            GCACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCG   <  SRR4302542.6378678/94‑1 (MQ=60)

                                                                                             CACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCGA  >  SRR4302542.706548/1‑94 (MQ=60)

CTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACACCGCCGGTGCTGGTACGCGA  >  NC_000913/2856191‑2856377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`