Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,888,375 A→G L516P (CTT→CCT)  cysJ ← sulfite reductase (NADPH)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,888,3750AG86.9% 671.9 / 90.7 274L516P (CTT→CCT) cysJsulfite reductase (NADPH)
Reads supporting (aligned to +/- strand):  ref base A (18/18);  new base G (127/111);  total (145/129)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 276 total aligned reads displayed.

CCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAG  >  NC_000913/2888285‑2888464
                                                                                          |                                                                                         
ccATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGa                                                                                         >  2:859999/1‑93 (MQ=255)
ccATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGa                                                                                         >  2:4466207/1‑93 (MQ=255)
ccATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGa                                                                                         >  2:1105646/1‑93 (MQ=255)
ccATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGa                                                                                         <  2:4291683/93‑1 (MQ=255)
 catcatTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGAt                                                                                        <  2:702758/93‑1 (MQ=255)
   tcatTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCg                                                                                      >  2:1624821/1‑93 (MQ=255)
    catTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGa                                                                                     >  2:5584427/1‑93 (MQ=255)
    catTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGa                                                                                     <  1:774035/93‑1 (MQ=255)
      ttGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATa                                                                                   <  2:2151388/93‑1 (MQ=255)
       tGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATAc                                                                                  <  1:301555/93‑1 (MQ=255)
         aTCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACgt                                                                                >  1:6915344/1‑93 (MQ=255)
         aTCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACgt                                                                                >  1:1700184/1‑93 (MQ=255)
          tCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACgtg                                                                               >  2:2705177/1‑93 (MQ=255)
          tCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACgtg                                                                               <  1:7507808/93‑1 (MQ=255)
           ccAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACgtgt                                                                              <  2:5751256/93‑1 (MQ=255)
           ccAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACgtgt                                                                              <  2:3444337/93‑1 (MQ=255)
            cAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTc                                                                             <  1:1619958/93‑1 (MQ=255)
            cAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTc                                                                             >  1:4648493/1‑93 (MQ=255)
             aGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCa                                                                            <  2:1891130/93‑1 (MQ=255)
             aGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCa                                                                            <  1:6418152/93‑1 (MQ=255)
              gcgcCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAg                                                                           >  1:866739/1‑93 (MQ=255)
              gcgcCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAg                                                                           >  1:1023030/1‑93 (MQ=255)
                gcCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCa                                                                         <  2:4679453/93‑1 (MQ=255)
                gcCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCa                                                                         >  2:758721/1‑93 (MQ=255)
                   acaGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGACACGTGTCAGCACGc                                                                      >  2:3549451/1‑93 (MQ=255)
                    caGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGcc                                                                     >  1:3484800/1‑93 (MQ=255)
                    caGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGcc                                                                     <  1:5759696/93‑1 (MQ=255)
                     aGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCa                                                                    <  2:2088942/93‑1 (MQ=255)
                     aGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCa                                                                    <  2:1326393/93‑1 (MQ=255)
                     aGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCa                                                                    >  1:2817974/1‑93 (MQ=255)
                      gCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAt                                                                   >  1:5032643/1‑93 (MQ=255)
                      gCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAt                                                                   <  2:5176824/93‑1 (MQ=255)
                       cTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATc                                                                  <  1:3567247/93‑1 (MQ=255)
                        tCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCt                                                                 >  2:297298/1‑93 (MQ=255)
                        tCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCt                                                                 <  1:3073801/93‑1 (MQ=255)
                         ccGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCtt                                                                <  2:6139217/93‑1 (MQ=255)
                         ccGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCtt                                                                <  2:3222125/93‑1 (MQ=255)
                          cgcgCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCttt                                                               <  2:586113/93‑1 (MQ=255)
                            cgcCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGa                                                             <  1:6826213/93‑1 (MQ=255)
                             gcCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGAc                                                            <  1:6937555/93‑1 (MQ=255)
                              cccTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACg                                                           >  1:4983362/1‑93 (MQ=255)
                               ccTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGt                                                          >  1:7400798/1‑93 (MQ=255)
                                cTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTa                                                         >  1:532551/1‑93 (MQ=255)
                                  gTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAgc                                                       >  1:3370074/1‑93 (MQ=255)
                                  gTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAgc                                                       >  1:6089768/1‑93 (MQ=255)
                                          aGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAc                                               >  2:149250/1‑93 (MQ=255)
                                          aGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAc                                               >  2:439469/1‑93 (MQ=255)
                                          aGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAc                                               >  1:4272/1‑93 (MQ=255)
                                          aGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAc                                               <  2:1885220/93‑1 (MQ=255)
                                           gTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACt                                              >  1:6464617/1‑93 (MQ=255)
                                            tttGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTc                                             >  1:5670076/1‑93 (MQ=255)
                                              tGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCa                                           <  2:5433585/93‑1 (MQ=255)
                                               gTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCAc                                          >  1:947544/1‑93 (MQ=255)
                                               gTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCAc                                          <  1:2041853/93‑1 (MQ=255)
                                                  ttGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTg                                       <  1:7152111/93‑1 (MQ=255)
                                                  ttGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTg                                       >  1:2260878/1‑93 (MQ=255)
                                                  ttGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTg                                       <  1:5703640/93‑1 (MQ=255)
                                                   tGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTgg                                      <  1:4554040/93‑1 (MQ=255)
                                                     tACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTa                                    <  1:3724848/93‑1 (MQ=255)
                                                      aCGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTAc                                   >  1:2634096/1‑93 (MQ=255)
                                                        gTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAg                                 >  2:4540561/1‑93 (MQ=255)
                                                        gTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAg                                 >  1:7176930/1‑93 (MQ=255)
                                                         tAAACTTTTTCTTTTTGATCGCGCGACCGGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAgg                                >  2:472761/1‑93 (MQ=255)
                                                         tAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAgg                                <  2:2415937/93‑1 (MQ=255)
                                                          aaaCTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGa                               <  2:6951617/93‑1 (MQ=255)
                                                           aaCTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGaa                              >  2:6691023/1‑93 (MQ=255)
                                                           aaCTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGaa                              <  1:1386072/93‑1 (MQ=255)
                                                           aaCTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGaa                              <  1:2601586/93‑1 (MQ=255)
                                                            aCTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAg                             <  1:4823809/93‑1 (MQ=255)
                                                             ctttttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAgc                            >  2:7278563/1‑92 (MQ=255)
                                                              tttttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTc                           >  1:3846565/1‑93 (MQ=255)
                                                                tttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCtt                         <  2:598159/93‑1 (MQ=255)
                                                                 ttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTc                        >  1:1142986/1‑93 (MQ=255)
                                                                 ttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTc                        <  1:1553099/93‑1 (MQ=255)
                                                                 ttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTc                        >  2:238534/1‑93 (MQ=255)
                                                                 ttctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTc                        <  1:4396037/93‑1 (MQ=255)
                                                                 ttctttttGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTc                        <  1:3819174/93‑1 (MQ=255)
                                                                  tctttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTcc                       >  2:7449276/1‑93 (MQ=255)
                                                                    tttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGt                     >  1:2110010/1‑93 (MQ=255)
                                                                    tttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGt                     <  1:1944264/93‑1 (MQ=255)
                                                                    tttttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTCACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGt                     >  1:236567/1‑93 (MQ=255)
                                                                    tttttGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGt                     >  2:4983462/1‑93 (MQ=255)
                                                                      tttGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTaa                   >  1:7454406/1‑93 (MQ=255)
                                                                        tGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAg                 >  1:6609219/1‑93 (MQ=255)
                                                                        tGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAg                 <  2:7100010/93‑1 (MQ=255)
                                                                         gATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGt                <  1:407808/93‑1 (MQ=255)
                                                                          aTCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTg               <  2:374336/93‑1 (MQ=255)
                                                                           tCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGc              <  2:2623894/93‑1 (MQ=255)
                                                                            cgcgcgACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCg             <  2:3898690/93‑1 (MQ=255)
                                                                              cgcgACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGa           <  2:6734233/93‑1 (MQ=255)
                                                                               gcgACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGAt          >  2:152649/1‑93 (MQ=255)
                                                                               gcgACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGCAAAGTGCGGAt          >  1:1429573/1‑93 (MQ=255)
                                                                                 gACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTa        >  2:5309233/1‑93 (MQ=255)
                                                                                 gACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTa        >  1:5997940/1‑93 (MQ=255)
                                                                                  aCCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTAc       <  2:1439567/93‑1 (MQ=255)
                                                                                    caggcaggATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCa     >  2:4106717/1‑93 (MQ=255)
                                                                                     aGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCaa    >  2:179228/1‑93 (MQ=255)
                                                                                      ggCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCaaa   >  2:6525884/1‑93 (MQ=255)
                                                                                      ggCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCaaa   >  2:6366851/1‑93 (MQ=255)
                                                                                      ggCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCaaa   <  2:5248876/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAG  >  NC_000913/2888285‑2888464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAGAAC  >  NC_000913/2888282‑2888467
                                                                                             |                                                                                            
GCACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAG                                                                                              <  SRR4302542.7466534/94‑1 (MQ=60)
GCACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAG                                                                                              <  SRR4302542.21249/94‑1 (MQ=60)
 CACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGG                                                                                             >  SRR4302542.4338603/1‑94 (MQ=60)
 CACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGG                                                                                             <  SRR4302542.6542449/94‑1 (MQ=60)
 CACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGG                                                                                             <  SRR4302542.396248/94‑1 (MQ=60)
  ACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGA                                                                                            <  SRR4302542.5011952/94‑1 (MQ=60)
   CCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGAT                                                                                           <  SRR4302542.1907436/94‑1 (MQ=60)
    CATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATC                                                                                          <  SRR4302542.6385116/94‑1 (MQ=60)
       CATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGAT                                                                                       <  SRR4302542.780718/94‑1 (MQ=60)
        ATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATA                                                                                      <  SRR4302542.892423/94‑1 (MQ=60)
          TGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACG                                                                                    <  SRR4302542.304182/94‑1 (MQ=60)
           GATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGT                                                                                   >  SRR4302542.6983228/1‑94 (MQ=60)
           GATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGT                                                                                   >  SRR4302542.1714918/1‑94 (MQ=60)
            ATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTG                                                                                  >  SRR4302542.4126056/1‑94 (MQ=60)
             TCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGT                                                                                 >  SRR4302542.6608106/1‑94 (MQ=60)
             TCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGT                                                                                 <  SRR4302542.7581648/94‑1 (MQ=60)
             TCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGT                                                                                 <  SRR4302542.5007738/94‑1 (MQ=60)
              CCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTC                                                                                >  SRR4302542.7242183/1‑94 (MQ=60)
              CCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTC                                                                                >  SRR4302542.4693942/1‑94 (MQ=60)
               CAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCA                                                                               <  SRR4302542.6955423/94‑1 (MQ=60)
               CAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCA                                                                               <  SRR4302542.1633953/94‑1 (MQ=60)
                AGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAG                                                                              >  SRR4302542.874207/1‑94 (MQ=60)
                AGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAG                                                                              >  SRR4302542.3856548/1‑94 (MQ=60)
                AGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAG                                                                              >  SRR4302542.1031845/1‑94 (MQ=60)
                AGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAG                                                                              <  SRR4302542.6481400/94‑1 (MQ=60)
                   GCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCAC                                                                           <  SRR4302542.3095940/94‑1 (MQ=60)
                   GCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCAC                                                                           >  SRR4302542.2188718/1‑94 (MQ=60)
                   GCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCAC                                                                           <  SRR4302542.812919/94‑1 (MQ=60)
                     CACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGC                                                                         >  SRR4302542.595263/1‑94 (MQ=60)
                     CACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGC                                                                         >  SRR4302542.5073259/1‑94 (MQ=60)
                      ACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCC                                                                        >  SRR4302542.3517401/1‑94 (MQ=60)
                       CAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCA                                                                       <  SRR4302542.5815605/94‑1 (MQ=60)
                       CAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCA                                                                       <  SRR4302542.5345770/94‑1 (MQ=60)
                       CAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCA                                                                       >  SRR4302542.2845078/1‑94 (MQ=60)
                        AGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAT                                                                      >  SRR4302542.4140071/1‑94 (MQ=60)
                        AGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAT                                                                      >  SRR4302542.2148842/1‑94 (MQ=60)
                        AGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAT                                                                      >  SRR4302542.5081497/1‑94 (MQ=60)
                        AGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCAT                                                                      >  SRR4302542.2866974/1‑94 (MQ=60)
                          CTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCT                                                                    <  SRR4302542.3600547/94‑1 (MQ=60)
                          CTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCT                                                                    <  SRR4302542.4326854/94‑1 (MQ=60)
                          CTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCT                                                                    >  SRR4302542.1515272/1‑94 (MQ=60)
                           TCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTT                                                                   <  SRR4302542.775992/94‑1 (MQ=60)
                           TCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTT                                                                   <  SRR4302542.3103094/94‑1 (MQ=60)
                           TCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTT                                                                   >  SRR4302542.3085491/1‑94 (MQ=60)
                            CCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTT                                                                  <  SRR4302542.1661755/94‑1 (MQ=60)
                            CCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTT                                                                  >  SRR4302542.3497824/1‑94 (MQ=60)
                            CCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTT                                                                  >  SRR4302542.2696321/1‑94 (MQ=60)
                            CCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTT                                                                  >  SRR4302542.6804500/1‑94 (MQ=60)
                             CGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTG                                                                 >  SRR4302542.2316193/1‑94 (MQ=60)
                             CGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTG                                                                 <  SRR4302542.7592886/94‑1 (MQ=60)
                               CGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGAC                                                               <  SRR4302542.6893303/94‑1 (MQ=60)
                                GCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACG                                                              <  SRR4302542.7005712/94‑1 (MQ=60)
                                GCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACG                                                              >  SRR4302542.5031721/1‑94 (MQ=60)
                                 CCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGT                                                             >  SRR4302542.7473224/1‑94 (MQ=60)
                                  CCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTA                                                            >  SRR4302542.537149/1‑94 (MQ=60)
                                   CTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAG                                                           >  SRR4302542.4398432/1‑94 (MQ=60)
                                   CTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAG                                                           <  SRR4302542.2060386/94‑1 (MQ=60)
                                    TGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGC                                                          >  SRR4302542.3401718/1‑94 (MQ=60)
                                    TGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGC                                                          >  SRR4302542.6148729/1‑94 (MQ=60)
                                    TGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGC                                                          >  SRR4302542.7279137/1‑94 (MQ=60)
                                     GTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCG                                                         >  SRR4302542.6719594/1‑94 (MQ=60)
                                     GTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCG                                                         >  SRR4302542.3865333/1‑94 (MQ=60)
                                       TCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCGTTGACGTAGCGCT                                                       <  SRR4302542.2635734/94‑1 (MQ=60)
                                         GCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGC                                                     <  SRR4302542.3598509/94‑1 (MQ=60)
                                          CGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCC                                                    >  SRR4302542.3895665/1‑94 (MQ=60)
                                           GCAGTTTGTCTTGTACGTAAAGTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATAAGTGTCAGCACGCCATCTTTGGCGTAGCGCTGCCA                                                   >  SRR4302542.1423404/1‑94 (MQ=60)
                                           GCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGGGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCA                                                   >  SRR4302542.7579880/1‑94 (MQ=60)
                                           GCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCA                                                   >  SRR4302542.7073815/1‑94 (MQ=60)
                                            CAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAC                                                  >  SRR4302542.4305/1‑94 (MQ=60)
                                            CAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCAC                                                  <  SRR4302542.732840/94‑1 (MQ=60)
                                             AGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACT                                                 <  SRR4302542.3621377/94‑1 (MQ=60)
                                             AGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACT                                                 >  SRR4302542.6528311/1‑94 (MQ=60)
                                              GTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTC                                                >  SRR4302542.5725147/1‑94 (MQ=60)
                                              GTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTC                                                >  SRR4302542.4072473/1‑94 (MQ=60)
                                               TTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCC                                               >  SRR4302542.3023975/1‑94 (MQ=60)
                                                 TGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCAC                                             >  SRR4302542.955689/1‑94 (MQ=60)
                                                  GTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGTCATCTTTGACGTAGCGCTGCCACTCCACC                                            <  SRR4302542.4658472/94‑1 (MQ=60)
                                                  GTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACC                                            <  SRR4302542.3881577/94‑1 (MQ=60)
                                                  GTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACC                                            <  SRR4302542.2059509/94‑1 (MQ=60)
                                                    CTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTG                                          >  SRR4302542.2280317/1‑94 (MQ=60)
                                                    CTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTG                                          >  SRR4302542.1434988/1‑94 (MQ=60)
                                                     TTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGG                                         <  SRR4302542.7222280/94‑1 (MQ=60)
                                                     TTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGG                                         <  SRR4302542.5759005/94‑1 (MQ=60)
                                                     TTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGG                                         <  SRR4302542.1976729/94‑1 (MQ=60)
                                                     TTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGG                                         <  SRR4302542.7514245/94‑1 (MQ=60)
                                                      TGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGT                                        <  SRR4302542.4598602/94‑1 (MQ=60)
                                                        TACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTAC                                      >  SRR4302542.2659539/1‑94 (MQ=60)
                                                        TACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTAC                                      <  SRR4302542.4767869/94‑1 (MQ=60)
                                                        TACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTAC                                      >  SRR4302542.3517253/1‑94 (MQ=60)
                                                        TACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTAC                                      <  SRR4302542.3761736/94‑1 (MQ=60)
                                                          CGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAG                                    <  SRR4302542.1452143/94‑1 (MQ=60)
                                                          CGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAG                                    >  SRR4302542.721983/1‑94 (MQ=60)
                                                          CGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAG                                    >  SRR4302542.7247333/1‑94 (MQ=60)
                                                           GTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGG                                   <  SRR4302542.1118993/94‑1 (MQ=60)
                                                           GTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGG                                   <  SRR4302542.7168648/94‑1 (MQ=60)
                                                            TAAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGA                                  >  SRR4302542.5814880/1‑94 (MQ=60)
                                                            TAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGA                                  <  SRR4302542.261684/94‑1 (MQ=60)
                                                             AAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAA                                 >  SRR4302542.7549686/1‑94 (MQ=60)
                                                             AAACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAA                                 >  SRR4302542.5636498/1‑94 (MQ=60)
                                                              AACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAG                                >  SRR4302542.5731898/1‑94 (MQ=60)
                                                              AACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAG                                <  SRR4302542.4878922/94‑1 (MQ=60)
                                                              AACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAG                                <  SRR4302542.1398044/94‑1 (MQ=60)
                                                              AACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAG                                <  SRR4302542.1791857/94‑1 (MQ=60)
                                                              AACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAG                                <  SRR4302542.2626750/94‑1 (MQ=60)
                                                               ACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGT                               <  SRR4302542.4870821/94‑1 (MQ=60)
                                                               ACTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGT                               <  SRR4302542.6508931/94‑1 (MQ=60)
                                                                CTTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTC                              >  SRR4302542.3884576/1‑94 (MQ=60)
                                                                 TTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCT                             >  SRR4302542.6089535/1‑94 (MQ=60)
                                                                 TTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCT                             <  SRR4302542.4586069/94‑1 (MQ=60)
                                                                 TTTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGGGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCT                             >  SRR4302542.876796/1‑94 (MQ=60)
                                                                  TTTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTT                            >  SRR4302542.3727504/1‑94 (MQ=60)
                                                                   TTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTC                           >  SRR4302542.4665342/1‑94 (MQ=60)
                                                                   TTTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTC                           >  SRR4302542.1152772/1‑94 (MQ=60)
                                                                   TTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTC                           >  SRR4302542.1196301/1‑94 (MQ=60)
                                                                    TTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCC                          <  SRR4302542.4439161/94‑1 (MQ=60)
                                                                    TTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCC                          <  SRR4302542.558046/94‑1 (MQ=60)
                                                                    TTCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCC                          <  SRR4302542.1566502/94‑1 (MQ=60)
                                                                    TTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCC                          <  SRR4302542.3856949/94‑1 (MQ=60)
                                                                     TCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCG                         <  SRR4302542.1682218/94‑1 (MQ=60)
                                                                     TCTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCG                         >  SRR4302542.6121299/1‑94 (MQ=60)
                                                                      CTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGT                        >  SRR4302542.2440570/1‑94 (MQ=60)
                                                                      CTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGT                        >  SRR4302542.2128222/1‑94 (MQ=60)
                                                                      CTTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTCACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGT                        >  SRR4302542.238600/1‑94 (MQ=60)
                                                                       TTTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTA                       <  SRR4302542.1961082/94‑1 (MQ=60)
                                                                        TTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAA                      <  SRR4302542.1585689/94‑1 (MQ=60)
                                                                        TTTTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAA                      >  SRR4302542.7527402/1‑94 (MQ=60)
                                                                          TTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAG                    <  SRR4302542.132844/94‑1 (MQ=60)
                                                                          TTGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAG                    >  SRR4302542.6674325/1‑94 (MQ=60)
                                                                           TGATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGT                   >  SRR4302542.3169961/1‑94 (MQ=60)
                                                                            GATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTG                  >  SRR4302542.6964464/1‑94 (MQ=60)
                                                                            GATCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTG                  <  SRR4302542.411332/94‑1 (MQ=60)
                                                                             ATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGC                 >  SRR4302542.2048316/1‑94 (MQ=60)
                                                                              TCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCG                <  SRR4302542.6638159/94‑1 (MQ=60)
                                                                              TCGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCG                <  SRR4302542.6389149/94‑1 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               >  SRR4302542.870902/1‑94 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               <  SRR4302542.992728/94‑1 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               >  SRR4302542.2768244/1‑94 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               <  SRR4302542.3587546/94‑1 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               >  SRR4302542.4742660/1‑94 (MQ=60)
                                                                               CGCGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGG               >  SRR4302542.7003298/1‑94 (MQ=60)
                                                                                 CGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGCAAAGTGCGGAT             >  SRR4302542.1441937/1‑94 (MQ=60)
                                                                                 CGCGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCATAAAGTGCGGAT             <  SRR4302542.5726414/94‑1 (MQ=60)
                                                                                   CGACCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTA           >  SRR4302542.6056029/1‑94 (MQ=60)
                                                                                   CGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTA           <  SRR4302542.6055224/94‑1 (MQ=60)
                                                                                      CCAGGCAGGATCGATACGTGTCAGCACGCCATCTTTAACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCA        >  SRR4302542.5372799/1‑94 (MQ=60)
                                                                                         GGCAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAG     >  SRR4302542.2370908/1‑94 (MQ=60)
                                                                                           CAGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAGAA   <  SRR4302542.2253608/94‑1 (MQ=60)
                                                                                            AGGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAGAAC  >  SRR4302542.2336203/1‑94 (MQ=60)
                                                                                             |                                                                                            
GCACCATCATTGATCCAGCGCCACAGCTCCGCGCCCTGTTCGCGCAGTTTGTCTTGTACGTAAACTTTTTCTTTTTGATCGCGCGACCAGGCAAGATCGATACGTGTCAGCACGCCATCTTTGACGTAGCGCTGCCACTCCACCTGGTACAGGAAGTCTTCCGTAAAGTGCGGATTACCAAAGAAC  >  NC_000913/2888282‑2888467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑