Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,900,224 T→C W103R (TGG→CGG)  ygcE → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,2240TC85.4% 694.3 / 108.1 294W103R (TGG→CGG) ygcEputative kinase
Reads supporting (aligned to +/- strand):  ref base T (32/11);  new base C (136/115);  total (168/126)
Fisher's exact test for biased strand distribution p-value = 1.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 296 total aligned reads displayed.

AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTA  >  NC_000913/2900133‑2900316
                                                                                           |                                                                                            
agaagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTg                                                                                             >  1:4159360/1‑93 (MQ=255)
  aagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGc                                                                                           >  1:4610818/1‑93 (MQ=255)
  aagaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGc                                                                                           >  2:763754/1‑93 (MQ=255)
   agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCa                                                                                          >  1:2307468/1‑93 (MQ=255)
   agaTATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCa                                                                                          <  2:4973490/93‑1 (MQ=255)
     atatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAgg                                                                                        >  1:3397380/1‑93 (MQ=255)
      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGa                                                                                       >  1:6535627/1‑93 (MQ=255)
      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGa                                                                                       >  2:3940116/1‑93 (MQ=255)
      tatTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGa                                                                                       >  1:5801550/1‑93 (MQ=255)
         tGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGc                                                                                    >  1:4303322/1‑93 (MQ=255)
           tCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAc                                                                                  >  2:688218/1‑93 (MQ=255)
             ggTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAcgc                                                                                <  2:4107876/93‑1 (MQ=255)
             ggTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAcgc                                                                                >  1:2864202/1‑93 (MQ=255)
               tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGt                                                                              >  2:2625393/1‑93 (MQ=255)
               tATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGt                                                                              >  2:1719933/1‑93 (MQ=255)
                aTTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGtt                                                                             >  1:5275045/1‑93 (MQ=255)
                aTTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGtt                                                                             >  2:5658550/1‑93 (MQ=255)
                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTa                                                                            >  1:1747039/1‑93 (MQ=255)
                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  2:6387634/1‑93 (MQ=255)
                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  2:6793655/1‑93 (MQ=255)
                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            >  1:5998002/1‑93 (MQ=255)
                 ttGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTa                                                                            <  2:6134404/93‑1 (MQ=255)
                  tGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTac                                                                           <  1:826164/93‑1 (MQ=255)
                    gTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTacac                                                                         <  1:5605269/93‑1 (MQ=255)
                      cTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGc                                                                       <  2:1213053/93‑1 (MQ=255)
                      cTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGc                                                                       >  1:405336/1‑93 (MQ=255)
                       tGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGcc                                                                      >  2:2999813/1‑93 (MQ=255)
                        gggATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGccc                                                                     <  2:3392204/93‑1 (MQ=255)
                              catccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGa                                                               >  1:3787045/1‑93 (MQ=255)
                               atccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGaa                                                              >  2:3671841/1‑93 (MQ=255)
                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  1:4678502/1‑93 (MQ=255)
                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  1:3667617/1‑93 (MQ=255)
                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  2:1796828/1‑93 (MQ=255)
                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             >  2:232818/1‑93 (MQ=255)
                                tccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAac                                                             <  1:1163588/93‑1 (MQ=255)
                                 ccGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAaca                                                            >  2:6017523/1‑93 (MQ=255)
                                  cGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAacac                                                           <  1:4827835/93‑1 (MQ=255)
                                      gctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTa                                                       >  1:7176876/1‑93 (MQ=255)
                                      gctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTa                                                       <  2:479726/93‑1 (MQ=255)
                                       ctgcCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTaa                                                      <  2:815041/93‑1 (MQ=255)
                                          ccGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc                                                   <  1:5183289/93‑1 (MQ=255)
                                          ccGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAAccc                                                   >  2:2407899/1‑93 (MQ=255)
                                           cGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                                                  >  2:4604158/1‑93 (MQ=255)
                                           cGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCt                                                  >  1:574452/1‑93 (MQ=255)
                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  2:739910/1‑93 (MQ=255)
                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  1:1473104/1‑93 (MQ=255)
                                            gTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTg                                                 >  1:1026106/1‑93 (MQ=255)
                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  1:1220238/93‑1 (MQ=255)
                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  2:4899745/93‑1 (MQ=255)
                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                >  1:5512711/1‑93 (MQ=255)
                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                >  1:202203/1‑93 (MQ=255)
                                             tGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGa                                                <  2:2769659/93‑1 (MQ=255)
                                              gCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAc                                               <  1:2812147/93‑1 (MQ=255)
                                              gCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAc                                               >  1:5357502/1‑93 (MQ=255)
                                               cGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACg                                              >  1:1300283/1‑93 (MQ=255)
                                                gTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGt                                             >  2:2831644/1‑93 (MQ=255)
                                                gTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGt                                             >  2:4225095/1‑93 (MQ=255)
                                                    ttGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCa                                         <  2:1986164/93‑1 (MQ=255)
                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      <  2:891347/93‑1 (MQ=255)
                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      >  1:7299776/1‑93 (MQ=255)
                                                       aaaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCatat                                      >  2:4339557/1‑93 (MQ=255)
                                                         aGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGt                                    <  1:4933335/93‑1 (MQ=255)
                                                          gCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTc                                   <  2:3597052/93‑1 (MQ=255)
                                                            cGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAc                                 >  2:5399111/1‑93 (MQ=255)
                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                >  1:2745349/1‑93 (MQ=255)
                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                <  1:3044975/93‑1 (MQ=255)
                                                             gATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAcc                                >  2:344783/1‑93 (MQ=255)
                                                              aTGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACct                               >  2:5844908/1‑93 (MQ=255)
                                                               tGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACctc                              <  1:4392584/93‑1 (MQ=255)
                                                               tGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACctc                              <  1:2652262/93‑1 (MQ=255)
                                                                 gCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCtt                            <  1:5899423/93‑1 (MQ=255)
                                                                    cGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCttttt                         <  1:4735052/93‑1 (MQ=255)
                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        >  1:7209609/1‑93 (MQ=255)
                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        >  2:1414533/1‑93 (MQ=255)
                                                                     gCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTc                        <  2:400365/93‑1 (MQ=255)
                                                                        tgctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     <  2:6058430/93‑1 (MQ=255)
                                                                        tgctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     >  1:1877471/1‑93 (MQ=255)
                                                                        tgctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCggg                     >  2:531609/1‑93 (MQ=255)
                                                                         gctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  2:323281/1‑93 (MQ=255)
                                                                         gctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  2:330725/1‑93 (MQ=255)
                                                                         gctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGt                    >  1:2189956/1‑93 (MQ=255)
                                                                          ctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGtt                   <  2:3771873/93‑1 (MQ=255)
                                                                          ctgcGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGtt                   <  2:6748096/93‑1 (MQ=255)
                                                                           tgcGCCATTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTa                  >  2:6989019/1‑93 (MQ=255)
                                                                            gcgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAt                 <  1:6397633/93‑1 (MQ=255)
                                                                            gcgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAt                 >  1:4552458/1‑93 (MQ=255)
                                                                             cgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCCCCTCTTTTTCGGGTTATc                <  1:1537478/93‑1 (MQ=255)
                                                                             cgcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATc                <  1:7435989/93‑1 (MQ=255)
                                                                              gcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTctct               <  1:6971158/93‑1 (MQ=255)
                                                                              gcCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCt               >  1:6807928/1‑93 (MQ=255)
                                                                               ccGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTg              <  1:1061182/93‑1 (MQ=255)
                                                                                 gTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  1:4710321/1‑93 (MQ=255)
                                                                                 gTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  2:5075510/1‑93 (MQ=255)
                                                                                 gTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAc            >  2:2280432/1‑93 (MQ=255)
                                                                                    gATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCa         <  1:6061455/93‑1 (MQ=255)
                                                                                    gATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCa         >  1:3966593/1‑93 (MQ=255)
                                                                                     aTTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCAt        >  1:994839/1‑93 (MQ=255)
                                                                                       tAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCg      >  2:6593004/1‑93 (MQ=255)
                                                                                        aGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGc     <  1:5091019/93‑1 (MQ=255)
                                                                                           tGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTa  <  2:5220773/93‑1 (MQ=255)
                                                                                           |                                                                                            
AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTA  >  NC_000913/2900133‑2900316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAA  >  NC_000913/2900131‑2900317
                                                                                             |                                                                                             
AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCC                                                                                               >  SRR4302542.1184715/1‑94 (MQ=60)
AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCC                                                                                               <  SRR4302542.59037/94‑1 (MQ=60)
 AAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTG                                                                                              >  SRR4302542.4200319/1‑94 (MQ=60)
 AAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGACTAGCCG                                                                                              >  SRR4302542.2377265/1‑94 (MQ=60)
  AGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGG                                                                                             >  SRR4302542.5622766/1‑94 (MQ=60)
   GAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGC                                                                                            >  SRR4302542.4655876/1‑94 (MQ=60)
   GAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGC                                                                                            >  SRR4302542.7421001/1‑94 (MQ=60)
   GAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGC                                                                                            >  SRR4302542.7362924/1‑94 (MQ=60)
    AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCA                                                                                           >  SRR4302542.2327286/1‑94 (MQ=60)
    AAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCA                                                                                           <  SRR4302542.7274197/94‑1 (MQ=60)
      GATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGG                                                                                         >  SRR4302542.3429250/1‑94 (MQ=60)
       ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGA                                                                                        >  SRR4302542.6600039/1‑94 (MQ=60)
       ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGA                                                                                        >  SRR4302542.5857851/1‑94 (MQ=60)
       ATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGA                                                                                        >  SRR4302542.4658513/1‑94 (MQ=60)
        TATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGAT                                                                                       <  SRR4302542.1410653/94‑1 (MQ=60)
        TATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGAT                                                                                       >  SRR4302542.7169167/1‑94 (MQ=60)
        TATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGAT                                                                                       >  SRR4302542.6113534/1‑94 (MQ=60)
        TATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGAT                                                                                       >  SRR4302542.285745/1‑94 (MQ=60)
        TATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCCGCGCCGTTGATTAGCTGGCAGGAT                                                                                       >  SRR4302542.3686765/1‑94 (MQ=60)
         ATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATG                                                                                      <  SRR4302542.2011547/94‑1 (MQ=60)
         ATTGTCGGTATTGGTCTGGGATCCATCCGTCGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATG                                                                                      <  SRR4302542.3626804/94‑1 (MQ=60)
          TTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGC                                                                                     >  SRR4302542.4345628/1‑94 (MQ=60)
           TGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCA                                                                                    >  SRR4302542.2882430/1‑94 (MQ=60)
           TGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCA                                                                                    >  SRR4302542.4820346/1‑94 (MQ=60)
           TGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCA                                                                                    >  SRR4302542.200530/1‑94 (MQ=60)
            GTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCAC                                                                                   >  SRR4302542.4085817/1‑94 (MQ=60)
             TCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACG                                                                                  <  SRR4302542.6995945/94‑1 (MQ=60)
              CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGC                                                                                 >  SRR4302542.3870002/1‑94 (MQ=60)
              CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGC                                                                                 >  SRR4302542.415823/1‑94 (MQ=60)
              CGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGC                                                                                 >  SRR4302542.2891716/1‑94 (MQ=60)
                GTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGT                                                                               >  SRR4302542.1680653/1‑94 (MQ=60)
                 TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTT                                                                              >  SRR4302542.2822705/1‑94 (MQ=60)
                 TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTT                                                                              >  SRR4302542.5326306/1‑94 (MQ=60)
                 TATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTT                                                                              >  SRR4302542.5220357/1‑94 (MQ=60)
                  ATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTA                                                                             >  SRR4302542.1762197/1‑94 (MQ=60)
                  ATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTA                                                                             >  SRR4302542.6056092/1‑94 (MQ=60)
                  ATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTA                                                                             >  SRR4302542.6556051/1‑94 (MQ=60)
                  ATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTA                                                                             >  SRR4302542.6631080/1‑94 (MQ=60)
                   TTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTAC                                                                            <  SRR4302542.2148533/94‑1 (MQ=60)
                    TGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACA                                                                           <  SRR4302542.833306/94‑1 (MQ=60)
                    TGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACA                                                                           <  SRR4302542.910288/94‑1 (MQ=60)
                     GGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACAC                                                                          <  SRR4302542.1195628/94‑1 (MQ=60)
                      GTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACG                                                                         <  SRR4302542.5659724/94‑1 (MQ=60)
                       TCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGC                                                                        >  SRR4302542.408837/1‑94 (MQ=60)
                        CTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCC                                                                       >  SRR4302542.4778867/1‑94 (MQ=60)
                        CTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCC                                                                       >  SRR4302542.582888/1‑94 (MQ=60)
                         TGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCC                                                                      <  SRR4302542.2222680/94‑1 (MQ=60)
                           GGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTT                                                                    >  SRR4302542.4381832/1‑94 (MQ=60)
                           GGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTT                                                                    >  SRR4302542.944597/1‑94 (MQ=60)
                           GGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTT                                                                    >  SRR4302542.1119316/1‑94 (MQ=60)
                             ATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTAC                                                                  <  SRR4302542.4300943/94‑1 (MQ=60)
                             ATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTAC                                                                  <  SRR4302542.6197417/94‑1 (MQ=60)
                              TCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGTATGCACGCGTTACACGCCCGTACG                                                                 <  SRR4302542.3744193/94‑1 (MQ=60)
                              TCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACG                                                                 >  SRR4302542.5843019/1‑94 (MQ=60)
                               CCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGA                                                                >  SRR4302542.5271542/1‑94 (MQ=60)
                               CCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGA                                                                >  SRR4302542.7352082/1‑94 (MQ=60)
                               CCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGA                                                                >  SRR4302542.3824495/1‑94 (MQ=60)
                                CATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAA                                                               >  SRR4302542.3270132/1‑94 (MQ=60)
                                CATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAA                                                               >  SRR4302542.5863680/1‑94 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              >  SRR4302542.4624790/1‑94 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              >  SRR4302542.4724219/1‑94 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              <  SRR4302542.1507488/94‑1 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              >  SRR4302542.3702629/1‑94 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              <  SRR4302542.4491516/94‑1 (MQ=60)
                                 ATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAAC                                                              <  SRR4302542.4767593/94‑1 (MQ=60)
                                  TCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACA                                                             >  SRR4302542.2689987/1‑94 (MQ=60)
                                  TCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACA                                                             <  SRR4302542.1173594/94‑1 (MQ=60)
                                    CGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACA                                                           >  SRR4302542.6918219/1‑94 (MQ=60)
                                    CGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACA                                                           <  SRR4302542.4874878/94‑1 (MQ=60)
                                     GTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                                          >  SRR4302542.4549368/1‑94 (MQ=60)
                                     GTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACAC                                                          <  SRR4302542.1253701/94‑1 (MQ=60)
                                      TTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACT                                                         <  SRR4302542.1662277/94‑1 (MQ=60)
                                       TGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTA                                                        >  SRR4302542.7247279/1‑94 (MQ=60)
                                        GCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAA                                                       >  SRR4302542.1228831/1‑94 (MQ=60)
                                          TGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACC                                                     <  SRR4302542.6781782/94‑1 (MQ=60)
                                          TGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACC                                                     >  SRR4302542.1532585/1‑94 (MQ=60)
                                           GCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCC                                                    <  SRR4302542.1771768/94‑1 (MQ=60)
                                            CCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                                                   >  SRR4302542.579384/1‑94 (MQ=60)
                                            CCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                                                   <  SRR4302542.3750877/94‑1 (MQ=60)
                                            CCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCT                                                   <  SRR4302542.5233648/94‑1 (MQ=60)
                                             CGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTG                                                  >  SRR4302542.5515257/1‑94 (MQ=60)
                                             CGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTG                                                  >  SRR4302542.1034949/1‑94 (MQ=60)
                                             CGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTG                                                  >  SRR4302542.1485820/1‑94 (MQ=60)
                                              GTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGA                                                 >  SRR4302542.5566282/1‑94 (MQ=60)
                                              GTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGA                                                 >  SRR4302542.5125973/1‑94 (MQ=60)
                                              GTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGA                                                 >  SRR4302542.203917/1‑94 (MQ=60)
                                               TGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                                                >  SRR4302542.5409572/1‑94 (MQ=60)
                                               TGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                                                <  SRR4302542.6115080/94‑1 (MQ=60)
                                               TGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                                                <  SRR4302542.4752256/94‑1 (MQ=60)
                                               TGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGAC                                                <  SRR4302542.1230739/94‑1 (MQ=60)
                                                GCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACG                                               >  SRR4302542.1311479/1‑94 (MQ=60)
                                                GCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACG                                               <  SRR4302542.2839208/94‑1 (MQ=60)
                                                GCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACG                                               <  SRR4302542.4943767/94‑1 (MQ=60)
                                                 CGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGT                                              >  SRR4302542.5168972/1‑94 (MQ=60)
                                                  GTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTG                                             >  SRR4302542.7448965/1‑94 (MQ=60)
                                                      TTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCAT                                         <  SRR4302542.5982349/94‑1 (MQ=60)
                                                       TGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATA                                        >  SRR4302542.5342418/1‑94 (MQ=60)
                                                       TGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATA                                        >  SRR4302542.4051693/1‑94 (MQ=60)
                                                        GAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATAT                                       >  SRR4302542.7371306/1‑94 (MQ=60)
                                                         AAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATG                                      <  SRR4302542.1340030/94‑1 (MQ=60)
                                                         AAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATG                                      <  SRR4302542.3099685/94‑1 (MQ=60)
                                                          AAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGT                                     <  SRR4302542.6005866/94‑1 (MQ=60)
                                                          AAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGT                                     <  SRR4302542.2791288/94‑1 (MQ=60)
                                                           AGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTC                                    <  SRR4302542.4981291/94‑1 (MQ=60)
                                                           AGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTC                                    <  SRR4302542.4970920/94‑1 (MQ=60)
                                                             CCGATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCAC                                  >  SRR4302542.5556485/1‑94 (MQ=60)
                                                              CGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACC                                 >  SRR4302542.2771817/1‑94 (MQ=60)
                                                               GATGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCT                                <  SRR4302542.3074046/94‑1 (MQ=60)
                                                                 TGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCT                              <  SRR4302542.2677876/94‑1 (MQ=60)
                                                                 TGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCT                              <  SRR4302542.267662/94‑1 (MQ=60)
                                                                 TGGCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCT                              <  SRR4302542.4435681/94‑1 (MQ=60)
                                                                  GGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTT                             <  SRR4302542.1118219/94‑1 (MQ=60)
                                                                   GCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT                            <  SRR4302542.5956618/94‑1 (MQ=60)
                                                                   GCACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTT                            <  SRR4302542.2636883/94‑1 (MQ=60)
                                                                    CACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTT                           >  SRR4302542.2223319/1‑94 (MQ=60)
                                                                    CACGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTT                           <  SRR4302542.1248643/94‑1 (MQ=60)
                                                                      CGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTC                         <  SRR4302542.4093635/94‑1 (MQ=60)
                                                                      CGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTC                         <  SRR4302542.3979321/94‑1 (MQ=60)
                                                                      CGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTC                         <  SRR4302542.2177551/94‑1 (MQ=60)
                                                                      CGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTC                         >  SRR4302542.7280357/1‑94 (MQ=60)
                                                                      CGCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTC                         <  SRR4302542.4781224/94‑1 (MQ=60)
                                                                       GCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCG                        >  SRR4302542.4986628/1‑94 (MQ=60)
                                                                       GCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCG                        >  SRR4302542.7048817/1‑94 (MQ=60)
                                                                       GCCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCG                        >  SRR4302542.3068736/1‑94 (MQ=60)
                                                                        CCTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGG                       <  SRR4302542.7275570/94‑1 (MQ=60)
                                                                         CTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGG                      >  SRR4302542.6131908/1‑94 (MQ=60)
                                                                         CTGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGG                      >  SRR4302542.1893718/1‑94 (MQ=60)
                                                                          TGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGT                     >  SRR4302542.2208817/1‑94 (MQ=60)
                                                                          TGCTGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGT                     <  SRR4302542.6549675/94‑1 (MQ=60)
                                                                             TGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAT                  <  SRR4302542.4674052/94‑1 (MQ=60)
                                                                             TGCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTAT                  >  SRR4302542.4597007/1‑94 (MQ=60)
                                                                              GCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATC                 <  SRR4302542.6460667/94‑1 (MQ=60)
                                                                              GCGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATC                 >  SRR4302542.5304000/1‑94 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCCCCTCTTTTTCGGGTTATCT                <  SRR4302542.1550758/94‑1 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCT                <  SRR4302542.5327166/94‑1 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCT                <  SRR4302542.5736199/94‑1 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCT                <  SRR4302542.7508766/94‑1 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCT                >  SRR4302542.6874862/1‑94 (MQ=60)
                                                                               CGCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCT                <  SRR4302542.1048500/94‑1 (MQ=60)
                                                                                GCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTCTCTG               <  SRR4302542.7039636/94‑1 (MQ=60)
                                                                                GCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTG               <  SRR4302542.7523219/94‑1 (MQ=60)
                                                                                GCCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTG               >  SRR4302542.5860240/1‑94 (MQ=60)
                                                                                 CCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGA              <  SRR4302542.1070306/94‑1 (MQ=60)
                                                                                 CCGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGA              >  SRR4302542.556237/1‑94 (MQ=60)
                                                                                  CGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAC             >  SRR4302542.4756259/1‑94 (MQ=60)
                                                                                  CGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAC             >  SRR4302542.427791/1‑94 (MQ=60)
                                                                                  CGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAC             <  SRR4302542.4372768/94‑1 (MQ=60)
                                                                                  CGTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGAC             <  SRR4302542.5789994/94‑1 (MQ=60)
                                                                                   GTTGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACG            <  SRR4302542.4830948/94‑1 (MQ=60)
                                                                                     TGATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCA          >  SRR4302542.4005755/1‑94 (MQ=60)
                                                                                      GATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCAT         <  SRR4302542.6120129/94‑1 (MQ=60)
                                                                                      GATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCAT         >  SRR4302542.1003401/1‑94 (MQ=60)
                                                                                       ATTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATC        >  SRR4302542.17956/1‑94 (MQ=60)
                                                                                        TTAGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCG       <  SRR4302542.2417186/94‑1 (MQ=60)
                                                                                          AGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCT     <  SRR4302542.5140501/94‑1 (MQ=60)
                                                                                          AGCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCT     <  SRR4302542.3006226/94‑1 (MQ=60)
                                                                                           GCCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTT    <  SRR4302542.4654828/94‑1 (MQ=60)
                                                                                            CCGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTA   <  SRR4302542.4578183/94‑1 (MQ=60)
                                                                                             TGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAA  <  SRR4302542.4732193/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAAGAAGATATTGTCGGTATTGGTCTGGGATCCATCCGTTGCTGCCGTGCGTTATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAA  >  NC_000913/2900131‑2900317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑