Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,929,074 A→G intergenic (+109/‑3) sdaB → / → b2798 L‑serine dehydratase/Exo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,929,0740AG85.2% 687.6 / 113.7 304intergenic (+109/‑3)sdaB/b2798L‑serine dehydratase/Exo
Reads supporting (aligned to +/- strand):  ref base A (16/29);  new base G (121/137);  total (137/167)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 305 total aligned reads displayed.

TCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928982‑2929165
                                                                                            |                                                                                           
    tttGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGt                                                                                         <  2:7149148/93‑1 (MQ=255)
     tggCGGGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTg                                                                                        <  1:2679537/91‑1 (MQ=255)
      tGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTgg                                                                                       >  1:468267/1‑93 (MQ=255)
       gCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGc                                                                                      <  2:6777918/93‑1 (MQ=255)
       cgggggCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGc                                                                                      <  1:2946611/89‑1 (MQ=255)
         gAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTg                                                                                    >  1:5038317/1‑93 (MQ=255)
             ccTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCa                                                                                <  1:6498564/93‑1 (MQ=255)
             ccTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCa                                                                                <  1:868402/93‑1 (MQ=255)
              tcctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAt                                                                               <  1:3717150/91‑1 (MQ=255)
              ctctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAt                                                                               >  2:6108933/1‑93 (MQ=255)
                ctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAttt                                                                             <  1:3772444/93‑1 (MQ=255)
                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  2:5606605/1‑93 (MQ=255)
                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  2:5836818/1‑93 (MQ=255)
                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       <  2:2338405/93‑1 (MQ=255)
                      gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                       >  1:6691226/1‑93 (MQ=255)
                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  2:3772432/93‑1 (MQ=255)
                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  1:7439239/93‑1 (MQ=255)
                       aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                      <  2:7225724/93‑1 (MQ=255)
                         ttCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGt                                                                    <  1:453480/93‑1 (MQ=255)
                         ttCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGt                                                                    <  2:5513066/93‑1 (MQ=255)
                           ctcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCg                                                                  >  2:5114215/1‑93 (MQ=255)
                            tcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGa                                                                 <  2:6609970/93‑1 (MQ=255)
                             cATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGAt                                                                <  2:3574496/93‑1 (MQ=255)
                              aTCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATg                                                               >  1:1178248/1‑93 (MQ=255)
                              aTCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATg                                                               <  2:4254757/93‑1 (MQ=255)
                               tCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGc                                                              <  1:2147526/93‑1 (MQ=255)
                                ccAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCa                                                             <  2:5667448/93‑1 (MQ=255)
                                ccAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCa                                                             <  1:236454/93‑1 (MQ=255)
                                 cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                            <  2:1849173/93‑1 (MQ=255)
                                 cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                            <  1:6420791/93‑1 (MQ=255)
                                  aGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACt                                                           <  1:2462945/93‑1 (MQ=255)
                                  aGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACt                                                           <  1:7343821/93‑1 (MQ=255)
                                   gCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTg                                                          <  1:5596630/93‑1 (MQ=255)
                                      gTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAAt                                                       <  1:5342592/93‑1 (MQ=255)
                                      gTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAAt                                                       >  2:4174911/1‑93 (MQ=255)
                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      <  1:7222205/93‑1 (MQ=255)
                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      >  2:283504/1‑93 (MQ=255)
                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      >  2:2407257/1‑93 (MQ=255)
                                       tAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATc                                                      <  2:6919250/93‑1 (MQ=255)
                                        aGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCt                                                     <  1:5367166/93‑1 (MQ=255)
                                         gCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCtt                                                    >  1:5112755/1‑93 (MQ=255)
                                          ccTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTa                                                   >  2:4804581/1‑93 (MQ=255)
                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  <  1:4847253/93‑1 (MQ=255)
                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  >  2:4861920/1‑93 (MQ=255)
                                           cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                  <  1:3972178/93‑1 (MQ=255)
                                            tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                                 >  1:7000778/1‑93 (MQ=255)
                                            tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                                 <  1:7190693/93‑1 (MQ=255)
                                             gTTCCGGCATCGAATGTTACCCTTATCTCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATCTGCTTATTGTCGATGCACTGAATCTTATtc                                                <  2:1618883/93‑1 (MQ=255)
                                             gTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtc                                                <  1:1736887/93‑1 (MQ=255)
                                              ttCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAttag                                               >  2:1243654/1‑91 (MQ=255)
                                                ccGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcgtc                                             <  2:668869/93‑1 (MQ=255)
                                                ccGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcgtc                                             <  2:4067994/93‑1 (MQ=255)
                                                  ggCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGc                                           >  1:5560625/1‑93 (MQ=255)
                                                  ggCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTAATTGTCGATGCACTGAATCTTATTCGTCGc                                           >  2:7434016/1‑93 (MQ=255)
                                                   gCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCa                                          <  2:1543467/93‑1 (MQ=255)
                                                   gCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCa                                          <  1:4314261/93‑1 (MQ=255)
                                                     aTCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAtt                                        >  1:1888702/1‑93 (MQ=255)
                                                     aTCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAtt                                        >  2:439085/1‑93 (MQ=255)
                                                      tCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTc                                       >  2:383679/1‑93 (MQ=255)
                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:298677/93‑1 (MQ=255)
                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:7011209/93‑1 (MQ=255)
                                                       cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                      <  2:3347566/93‑1 (MQ=255)
                                                          aTGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGc                                   <  2:346431/93‑1 (MQ=255)
                                                           tGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGcc                                  >  1:2900322/1‑93 (MQ=255)
                                                           tGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGcc                                  <  1:7218934/93‑1 (MQ=255)
                                                               aCCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTc                              <  2:4293775/93‑1 (MQ=255)
                                                                cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                             >  1:3077954/1‑93 (MQ=255)
                                                                cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                             >  1:321452/1‑93 (MQ=255)
                                                                  cTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgg                           >  2:2675736/1‑93 (MQ=255)
                                                                  cTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgg                           >  2:5782190/1‑93 (MQ=255)
                                                                   ttATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                          >  2:3118915/1‑93 (MQ=255)
                                                                   ttATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                          >  2:6858254/1‑93 (MQ=255)
                                                                    tATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgggg                         >  1:4762688/1‑93 (MQ=255)
                                                                     aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                        <  2:6134181/93‑1 (MQ=255)
                                                                     aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                        >  2:2495057/1‑93 (MQ=255)
                                                                      tCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTc                       <  2:4443808/93‑1 (MQ=255)
                                                                      tCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTc                       >  2:394626/1‑93 (MQ=255)
                                                                       cGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCg                      <  1:131915/93‑1 (MQ=255)
                                                                        gCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGc                     >  1:349870/1‑93 (MQ=255)
                                                                        gCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGc                     <  1:1653678/93‑1 (MQ=255)
                                                                         ccTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTCCAGGGGTCGcc                    <  2:4658035/93‑1 (MQ=255)
                                                                          cTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGccc                   <  1:4891676/93‑1 (MQ=255)
                                                                           tGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                  >  2:7337227/1‑93 (MQ=255)
                                                                           tGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                  <  1:6937294/93‑1 (MQ=255)
                                                                            gATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtg                 >  1:3373522/1‑93 (MQ=255)
                                                                             aTCTTTAAGGGGGTGATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgt                >  2:6449273/1‑93 (MQ=255)
                                                                              tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg               <  1:305175/93‑1 (MQ=255)
                                                                              tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg               >  2:147036/1‑93 (MQ=255)
                                                                                tttAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTc             <  1:5183921/93‑1 (MQ=255)
                                                                                  tAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGa           <  2:121929/93‑1 (MQ=255)
                                                                                   aaGGGGGTTGTCGTCGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGaa          >  2:5187521/1‑93 (MQ=255)
                                                                                    aGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGaaa         <  2:3706434/93‑1 (MQ=255)
                                                                                     ggggggTATCGGGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  1:1018082/1‑93 (MQ=255)
                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        <  2:5059327/93‑1 (MQ=255)
                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  1:6090175/1‑93 (MQ=255)
                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        <  2:1350853/93‑1 (MQ=255)
                                                                                     gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc        >  2:2696721/1‑93 (MQ=255)
                                                                                       gggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCt      <  1:6108956/93‑1 (MQ=255)
                                                                                         gTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGc    <  2:815197/93‑1 (MQ=255)
                                                                                           tATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCa  <  2:6406546/93‑1 (MQ=255)
                                                                                            |                                                                                           
TCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928982‑2929165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928981‑2929165
                                                                                             |                                                                                           
CTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTG                                                                                             >  SRR4302542.3136999/1‑94 (MQ=60)
CTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTG                                                                                             >  SRR4302542.3441183/1‑94 (MQ=60)
CTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTA                                                                                             >  SRR4302542.1434801/1‑94 (MQ=60)
CTCGTTTTGCGAGCCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTG                                                                                             <  SRR4302542.2948173/94‑1 (MQ=60)
CTCGTTTTGCGAGCCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTG                                                                                             <  SRR4302542.5481591/94‑1 (MQ=60)
CTCGTTTGGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTG                                                                                             <  SRR4302542.1673170/94‑1 (MQ=60)
  CGTTTTGGGGGCCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTC                                                                                           <  SRR4302542.2987059/94‑1 (MQ=60)
  CGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTC                                                                                           >  SRR4302542.6510362/1‑94 (MQ=60)
      TTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGG                                                                                       <  SRR4302542.740876/94‑1 (MQ=60)
      TTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGG                                                                                       >  SRR4302542.472327/1‑94 (MQ=60)
      TGGCGGGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGG                                                                                       <  SRR4302542.2705396/94‑1 (MQ=60)
        cgggGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCT                                                                                     <  SRR4302542.2974821/90‑1 (MQ=60)
        GCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCT                                                                                     <  SRR4302542.4574843/94‑1 (MQ=60)
         CGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTG                                                                                    >  SRR4302542.3558088/1‑94 (MQ=60)
         CGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTG                                                                                    >  SRR4302542.5087231/1‑94 (MQ=60)
              CCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAT                                                                               <  SRR4302542.6562613/94‑1 (MQ=60)
              CCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAT                                                                               <  SRR4302542.875891/94‑1 (MQ=60)
               tcCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATT                                                                              <  SRR4302542.3753920/92‑1 (MQ=60)
               CTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATT                                                                              >  SRR4302542.1102535/1‑94 (MQ=60)
                 CTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTG                                                                            <  SRR4302542.3809758/94‑1 (MQ=60)
                 CTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTG                                                                            <  SRR4302542.2045221/94‑1 (MQ=60)
                 CTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTG                                                                            >  SRR4302542.6162761/1‑94 (MQ=60)
                 CTCCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTG                                                                            <  SRR4302542.7562911/94‑1 (MQ=60)
                  TTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTGATTTGC                                                                           >  SRR4302542.388945/1‑94 (MQ=60)
                  TTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGC                                                                           >  SRR4302542.6858390/1‑94 (MQ=60)
                  TTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGC                                                                           >  SRR4302542.247889/1‑94 (MQ=60)
                    CCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTT                                                                         >  SRR4302542.7066410/1‑94 (MQ=60)
                    CCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTT                                                                         >  SRR4302542.6434846/1‑94 (MQ=60)
                     CCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTA                                                                        >  SRR4302542.4421795/1‑94 (MQ=60)
                      CGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAT                                                                       >  SRR4302542.6757137/1‑94 (MQ=60)
                       GATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATT                                                                      <  SRR4302542.4736458/94‑1 (MQ=60)
                       GATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATT                                                                      <  SRR4302542.5296657/94‑1 (MQ=60)
                        ATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTG                                                                     <  SRR4302542.7512044/94‑1 (MQ=60)
                        ATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTG                                                                     <  SRR4302542.3016272/94‑1 (MQ=60)
                         TTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTTCCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGT                                                                    <  SRR4302542.2714204/94‑1 (MQ=60)
                         TTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGT                                                                    <  SRR4302542.2207209/94‑1 (MQ=60)
                         TTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGT                                                                    >  SRR4302542.3103201/1‑94 (MQ=60)
                          TTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTC                                                                   <  SRR4302542.5205528/94‑1 (MQ=60)
                          TTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTC                                                                   <  SRR4302542.457395/94‑1 (MQ=60)
                          TTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTC                                                                   <  SRR4302542.673094/94‑1 (MQ=60)
                           TCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCG                                                                  <  SRR4302542.3235835/94‑1 (MQ=60)
                           TCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCG                                                                  <  SRR4302542.5221588/94‑1 (MQ=60)
                             TCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGAT                                                                <  SRR4302542.4898363/94‑1 (MQ=60)
                             TCATCCAGCCGCAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGAT                                                                <  SRR4302542.4178008/94‑1 (MQ=60)
                              CATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGTTG                                                               >  SRR4302542.3488304/1‑94 (MQ=60)
                              CATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATG                                                               >  SRR4302542.1188425/1‑94 (MQ=60)
                               ATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGC                                                              >  SRR4302542.1694778/1‑94 (MQ=60)
                                TCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCA                                                             <  SRR4302542.2166041/94‑1 (MQ=60)
                                 CCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAC                                                            >  SRR4302542.1301445/1‑94 (MQ=60)
                                 CCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAC                                                            <  SRR4302542.3015353/94‑1 (MQ=60)
                                 CCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCAC                                                            <  SRR4302542.238487/94‑1 (MQ=60)
                                  CAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACT                                                           <  SRR4302542.6484066/94‑1 (MQ=60)
                                   AGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTG                                                          <  SRR4302542.4654118/94‑1 (MQ=60)
                                   AGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTG                                                          <  SRR4302542.7415749/94‑1 (MQ=60)
                                   AGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTG                                                          <  SRR4302542.2486865/94‑1 (MQ=60)
                                    GCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGA                                                         <  SRR4302542.5651008/94‑1 (MQ=60)
                                    GCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGA                                                         <  SRR4302542.7431131/94‑1 (MQ=60)
                                    GCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGA                                                         <  SRR4302542.5399687/94‑1 (MQ=60)
                                      CGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAAT                                                       <  SRR4302542.2909074/94‑1 (MQ=60)
                                       GTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTTTCGTGGCTTTTCATTTGCTTATTGTCGATGCCCTGAATC                                                      <  SRR4302542.3693183/94‑1 (MQ=60)
                                       GTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATC                                                      >  SRR4302542.671935/1‑94 (MQ=60)
                                       GTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATC                                                      <  SRR4302542.5394493/94‑1 (MQ=60)
                                       GTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGAACTGAATC                                                      <  SRR4302542.5132485/94‑1 (MQ=60)
                                        TAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCT                                                     <  SRR4302542.2055607/94‑1 (MQ=60)
                                        TAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCT                                                     <  SRR4302542.7293049/94‑1 (MQ=60)
                                         AGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTT                                                    >  SRR4302542.5162411/1‑94 (MQ=60)
                                         AGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTT                                                    >  SRR4302542.7266084/1‑94 (MQ=60)
                                         AGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTT                                                    <  SRR4302542.5419327/94‑1 (MQ=60)
                                            CTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATT                                                 >  SRR4302542.7069549/1‑94 (MQ=60)
                                            CTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATT                                                 >  SRR4302542.5431487/1‑94 (MQ=60)
                                            CTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATT                                                 <  SRR4302542.4894455/94‑1 (MQ=60)
                                            CTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATT                                                 <  SRR4302542.2947181/94‑1 (MQ=60)
                                            CTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATT                                                 <  SRR4302542.4011376/94‑1 (MQ=60)
                                             TGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTC                                                <  SRR4302542.7261243/94‑1 (MQ=60)
                                             TGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTC                                                >  SRR4302542.7401/1‑94 (MQ=60)
                                              GTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCG                                               <  SRR4302542.1751957/94‑1 (MQ=60)
                                              GTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCG                                               >  SRR4302542.4124047/1‑94 (MQ=60)
                                               TTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGT                                              <  SRR4302542.3778385/94‑1 (MQ=60)
                                                TCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTC                                             <  SRR4302542.6077611/94‑1 (MQ=60)
                                                 CCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCG                                            >  SRR4302542.3271294/1‑94 (MQ=60)
                                                 CCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCG                                            <  SRR4302542.6523722/94‑1 (MQ=60)
                                                  CGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGC                                           >  SRR4302542.5614652/1‑94 (MQ=60)
                                                  CGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGC                                           >  SRR4302542.3758701/1‑94 (MQ=60)
                                                    GCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAT                                         <  SRR4302542.4356652/94‑1 (MQ=60)
                                                    GCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAT                                         >  SRR4302542.5081004/1‑94 (MQ=60)
                                                     CATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATT                                        >  SRR4302542.1905049/1‑94 (MQ=60)
                                                     CATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATT                                        >  SRR4302542.5139393/1‑94 (MQ=60)
                                                      ATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTC                                       >  SRR4302542.4284726/1‑94 (MQ=60)
                                                      ATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTC                                       >  SRR4302542.5395918/1‑94 (MQ=60)
                                                         GAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATG                                    >  SRR4302542.5595063/1‑94 (MQ=60)
                                                          AATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGC                                   <  SRR4302542.733498/94‑1 (MQ=60)
                                                          AATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGC                                   <  SRR4302542.264134/94‑1 (MQ=60)
                                                           ATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCC                                  <  SRR4302542.3199601/94‑1 (MQ=60)
                                                           ATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCC                                  >  SRR4302542.2928159/1‑94 (MQ=60)
                                                           ATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCC                                  >  SRR4302542.5754782/1‑94 (MQ=60)
                                                            TGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCG                                 <  SRR4302542.7289749/94‑1 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             >  SRR4302542.3107275/1‑94 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             >  SRR4302542.7535940/1‑94 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             <  SRR4302542.1871106/94‑1 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             >  SRR4302542.2961776/1‑94 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             <  SRR4302542.7290527/94‑1 (MQ=60)
                                                                ACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCA                             >  SRR4302542.324243/1‑94 (MQ=60)
                                                                 CCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAG                            <  SRR4302542.4640449/94‑1 (MQ=60)
                                                                 CCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAG                            >  SRR4302542.7292423/1‑94 (MQ=60)
                                                                 CCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAG                            >  SRR4302542.482396/1‑94 (MQ=60)
                                                                   CTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGG                          <  SRR4302542.7074136/94‑1 (MQ=60)
                                                                   CTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGG                          >  SRR4302542.6246471/1‑94 (MQ=60)
                                                                   CTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGG                          <  SRR4302542.7159142/94‑1 (MQ=60)
                                                                    TTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGG                         >  SRR4302542.4809119/1‑94 (MQ=60)
                                                                    TTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGG                         <  SRR4302542.1897036/94‑1 (MQ=60)
                                                                     TATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGT                        <  SRR4302542.5642189/94‑1 (MQ=60)
                                                                     TATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGT                        <  SRR4302542.7106840/94‑1 (MQ=60)
                                                                      ATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTC                       >  SRR4302542.6771280/1‑94 (MQ=60)
                                                                        CGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGC                     >  SRR4302542.352904/1‑94 (MQ=60)
                                                                        CGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGC                     <  SRR4302542.132992/94‑1 (MQ=60)
                                                                         GCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCC                    >  SRR4302542.5617559/1‑94 (MQ=60)
                                                                         GCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCC                    >  SRR4302542.4928014/1‑94 (MQ=60)
                                                                         GCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCC                    <  SRR4302542.5313313/94‑1 (MQ=60)
                                                                         GCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCC                    >  SRR4302542.6150727/1‑94 (MQ=60)
                                                                         GCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCC                    <  SRR4302542.1667962/94‑1 (MQ=60)
                                                                           CTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCT                  >  SRR4302542.1259530/1‑94 (MQ=60)
                                                                           CTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCT                  <  SRR4302542.4939247/94‑1 (MQ=60)
                                                                            TGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTtaaggggcggcccc                 >  SRR4302542.2437884/1‑80 (MQ=60)
                                                                            TGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTG                 >  SRR4302542.3405213/1‑94 (MQ=60)
                                                                            TGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTG                 <  SRR4302542.7005450/94‑1 (MQ=60)
                                                                             GATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGT                >  SRR4302542.143535/1‑94 (MQ=60)
                                                                              ATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTG               <  SRR4302542.3855376/94‑1 (MQ=60)
                                                                              ATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTG               <  SRR4302542.171782/94‑1 (MQ=60)
                                                                               TCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGT              <  SRR4302542.307838/94‑1 (MQ=60)
                                                                               TCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGT              >  SRR4302542.1133829/1‑94 (MQ=60)
                                                                                CTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTC             >  SRR4302542.7036496/1‑94 (MQ=60)
                                                                                CTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTC             >  SRR4302542.233566/1‑94 (MQ=60)
                                                                                 TTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCG            <  SRR4302542.5234289/94‑1 (MQ=60)
                                                                                    AAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAA         >  SRR4302542.3097965/1‑94 (MQ=60)
                                                                                     AGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAC        >  SRR4302542.4759925/1‑94 (MQ=60)
                                                                                     AGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAC        >  SRR4302542.7274283/1‑94 (MQ=60)
                                                                                     AGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAC        >  SRR4302542.6149136/1‑94 (MQ=60)
                                                                                     AGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAC        >  SRR4302542.4889444/1‑94 (MQ=60)
                                                                                     AGGGGGGTATCGGGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAC        >  SRR4302542.1026851/1‑94 (MQ=60)
                                                                                      GGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACC       >  SRR4302542.333555/1‑94 (MQ=60)
                                                                                      GGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACC       <  SRR4302542.2574291/94‑1 (MQ=60)
                                                                                        GGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTG     <  SRR4302542.6168079/94‑1 (MQ=60)
                                                                                        GGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTG     <  SRR4302542.3531252/94‑1 (MQ=60)
                                                                                          GTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCC   <  SRR4302542.5074064/94‑1 (MQ=60)
                                                                                           TTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  <  SRR4302542.2979998/94‑1 (MQ=60)
                                                                                           TTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  <  SRR4302542.2190164/94‑1 (MQ=60)
                                                                                             |                                                                                           
CTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGCCA  >  NC_000913/2928981‑2929165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑