Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,961,511	C→T	G76G (GGG→GGA)	ppdB ←	prepilin peptidase dependent protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,961,511	0	C	T	83.6%	717.0 / 97.6	274	G76G (GGG→GGA)	ppdB	prepilin peptidase dependent protein B
Reads supporting (aligned to +/- strand): ref base C (23/22); new base T (120/109); total (143/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-01

Only 100 of 279 total aligned reads displayed.

GGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGC  >  NC_000913/2961421‑2961603

ggACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGtcc                                                                                            <  2:6413899/93‑1 (MQ=255)

ggACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGccc                                                                                            <  1:5124565/93‑1 (MQ=255)

 gACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCt                                                                                           >  2:2568516/1‑93 (MQ=255)

  aCTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCtt                                                                                          >  1:7370704/1‑93 (MQ=255)

  aCTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCtt                                                                                          <  2:1684193/93‑1 (MQ=255)

    tctTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCg                                                                                        >  1:6513492/1‑93 (MQ=255)

     ctTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGc                                                                                       >  2:6839844/1‑93 (MQ=255)

      ttttACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGcc                                                                                      >  1:7262070/1‑93 (MQ=255)

       tttACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCg                                                                                     <  1:6284559/93‑1 (MQ=255)

          aCCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTa                                                                                  >  1:1269277/1‑93 (MQ=255)

           ccGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTAc                                                                                 <  1:3636508/93‑1 (MQ=255)

              gTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGa                                                                              <  2:2751691/93‑1 (MQ=255)

                 cgcgATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGc                                                                           <  1:5948871/93‑1 (MQ=255)

                  gcgATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGcc                                                                          <  1:6808861/93‑1 (MQ=255)

                    gATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCAt                                                                        >  2:7338031/1‑93 (MQ=255)

                    gATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCAt                                                                        >  2:3902251/1‑93 (MQ=255)

                    gATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCAt                                                                        >  2:5962196/1‑93 (MQ=255)

                      tCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGa                                                                      >  2:3487748/1‑93 (MQ=255)

                       cccAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGAc                                                                     >  2:1736746/1‑93 (MQ=255)

                         cAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACaa                                                                   <  1:6713237/93‑1 (MQ=255)

                          aGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAAt                                                                  >  2:4399823/1‑93 (MQ=255)

                           gATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATa                                                                 >  2:2805329/1‑93 (MQ=255)

                            aTACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATaa                                                                >  1:7475300/1‑93 (MQ=255)

                             tACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAc                                                               <  2:973000/93‑1 (MQ=255)

                             tACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAc                                                               >  1:5531140/1‑93 (MQ=255)

                             tACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAc                                                               >  2:5756609/1‑93 (MQ=255)

                             tACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAc                                                               <  1:4964885/93‑1 (MQ=255)

                             tACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAAc                                                               <  1:5706560/93‑1 (MQ=255)

                              aCCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              <  1:2549863/93‑1 (MQ=255)

                              aCCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              <  1:6465236/93‑1 (MQ=255)

                              aCCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              <  2:1918327/93‑1 (MQ=255)

                              aCCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              >  1:3370393/1‑93 (MQ=255)

                              aCCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              <  2:6013768/93‑1 (MQ=255)

                               ccGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAccc                                                             >  1:3234705/1‑93 (MQ=255)

                               ccGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAccc                                                             >  2:5648566/1‑93 (MQ=255)

                               ccGTTACTGTTCGCATCCAACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAccc                                                             >  1:4904878/1‑93 (MQ=255)

                                cGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCg                                                            >  2:2361442/1‑93 (MQ=255)

                                cGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCg                                                            <  1:5492938/93‑1 (MQ=255)

                                  ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGcc                                                          >  1:5304730/1‑93 (MQ=255)

                                  ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGcc                                                          >  1:6894337/1‑93 (MQ=255)

                                  ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGcc                                                          >  1:2030993/1‑93 (MQ=255)

                                   tACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGccc                                                         <  2:1802998/93‑1 (MQ=255)

                                    aCTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCct                                                        >  1:4381631/1‑93 (MQ=255)

                                    aCTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCct                                                        >  2:2887907/1‑93 (MQ=255)

                                    aCTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCct                                                        >  1:6631736/1‑93 (MQ=255)

                                    aCTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCct                                                        >  2:7290338/1‑93 (MQ=255)

                                     cTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCctc                                                       <  1:3469719/93‑1 (MQ=255)

                                      tGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCctct                                                      >  2:1039013/1‑93 (MQ=255)

                                      tGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCctct                                                      <  2:848591/93‑1 (MQ=255)

                                       gTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTg                                                     <  1:1866184/93‑1 (MQ=255)

                                         tCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGga                                                   >  1:4563768/1‑93 (MQ=255)

                                          cGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCTGGTACAGATGCCATGACAATAACCCGCCCTCTGgag                                                  >  1:4716340/1‑93 (MQ=255)

                                          cGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGgag                                                  >  1:4000100/1‑93 (MQ=255)

                                           gCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGgaga                                                 <  1:4793479/93‑1 (MQ=255)

                                             aTCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATg                                               >  2:514698/1‑93 (MQ=255)

                                             aTCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATg                                               >  1:4433992/1‑93 (MQ=255)

                                              tCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCTCCCTCTGGAGATGc                                              <  2:6437261/93‑1 (MQ=255)

                                              tCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGc                                              >  1:3609290/1‑93 (MQ=255)

                                               cccACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCt                                             >  1:1165425/1‑93 (MQ=255)

                                                ccACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCtt                                            <  1:1752894/93‑1 (MQ=255)

                                                ccACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCtt                                            <  2:5868473/93‑1 (MQ=255)

                                                 cACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTc                                           <  1:5200278/93‑1 (MQ=255)

                                                 cACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTc                                           <  2:7476828/93‑1 (MQ=255)

                                                 cACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTc                                           <  2:3670129/93‑1 (MQ=255)

                                                  aCTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcg                                          >  2:6141288/1‑93 (MQ=255)

                                                  aCTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcg                                          <  1:2301110/93‑1 (MQ=255)

                                                  aCTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcg                                          <  1:2342350/93‑1 (MQ=255)

                                                   cTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcgc                                         >  2:3348948/1‑93 (MQ=255)

                                                    tGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcgcg                                        <  1:4223165/93‑1 (MQ=255)

                                                     gCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGa                                       >  2:1089720/1‑93 (MQ=255)

                                                      cacaATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGAc                                      <  2:2263591/93‑1 (MQ=255)

                                                        caATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACgg                                    >  2:3525568/1‑93 (MQ=255)

                                                        caATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACgg                                    >  2:6949904/1‑93 (MQ=255)

                                                         aaTGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGt                                   >  1:4354775/1‑93 (MQ=255)

                                                          aTGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTa                                  >  2:6672714/1‑93 (MQ=255)

                                                           tGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTaa                                 <  1:6111802/93‑1 (MQ=255)

                                                            gACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTaaa                                <  1:7032239/93‑1 (MQ=255)

                                                            gACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTaaa                                <  1:3088622/93‑1 (MQ=255)

                                                             acacAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAt                               <  2:2456338/93‑1 (MQ=255)

                                                             acacAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAt                               <  2:65984/93‑1 (MQ=255)

                                                              cacaGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATa                              >  1:1077751/1‑93 (MQ=255)

                                                              cacaGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATa                              >  2:2090020/1‑93 (MQ=255)

                                                               acaGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATAc                             <  1:3751903/93‑1 (MQ=255)

                                                                  gTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCg                          >  2:5406111/1‑93 (MQ=255)

                                                                  gTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCg                          >  2:5060259/1‑93 (MQ=255)

                                                                  gTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCg                          >  2:1368510/1‑93 (MQ=255)

                                                                   tCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAcc                                                              <  1:1584063/56‑1 (MQ=255)

                                                                   tCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGc                         <  1:3930629/93‑1 (MQ=255)

                                                                       ccTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCTCCCTCTGGAGATGCTTCGCGACGGTAAATAACCGCAGcc                     >  1:771102/1‑93 (MQ=255)

                                                                        cTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCa                    <  2:524964/93‑1 (MQ=255)

                                                                         tGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAg                   <  1:3825102/93‑1 (MQ=255)

                                                                              gACAAGTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTc              >  2:4593818/1‑93 (MQ=255)

                                                                               aCAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCa             >  1:2457613/1‑93 (MQ=255)

                                                                               aCAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCa             >  1:2487115/1‑93 (MQ=255)

                                                                                  aTTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCt          >  2:3036642/1‑93 (MQ=255)

                                                                                    ttCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTc        <  2:4016474/93‑1 (MQ=255)

                                                                                     tCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTcc       <  1:100196/93‑1 (MQ=255)

                                                                                     tCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTcc       >  1:2869785/1‑93 (MQ=255)

                                                                                       cAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAg     >  1:1433356/1‑93 (MQ=255)

                                                                                          cccTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCAGCAGCCAGATTTCATCTTCCAgcgc  >  2:4210269/1‑93 (MQ=255)

GGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGC  >  NC_000913/2961421‑2961603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCC  >  NC_000913/2961418‑2961604

GTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGT                                                                                               <  SRR4302542.421187/94‑1 (MQ=60)

 TCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTC                                                                                              <  SRR4302542.4278844/94‑1 (MQ=60)

  CGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCC                                                                                             <  SRR4302542.927925/94‑1 (MQ=60)

  CGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCC                                                                                             >  SRR4302542.5876591/1‑94 (MQ=60)

   GGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCT                                                                                            >  SRR4302542.7148644/1‑94 (MQ=60)

   GGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCT                                                                                            >  SRR4302542.135367/1‑94 (MQ=60)

   GGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCT                                                                                            <  SRR4302542.5174326/94‑1 (MQ=60)

    GACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTT                                                                                           <  SRR4302542.440301/94‑1 (MQ=60)

    GACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTT                                                                                           >  SRR4302542.7442889/1‑94 (MQ=60)

    GACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTT                                                                                           >  SRR4302542.5265455/1‑94 (MQ=60)

    GACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTT                                                                                           <  SRR4302542.3139298/94‑1 (MQ=60)

     ACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTC                                                                                          <  SRR4302542.7022629/94‑1 (MQ=60)

     ACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTC                                                                                          <  SRR4302542.2530379/94‑1 (MQ=60)

     ACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTC                                                                                          >  SRR4302542.1511819/1‑94 (MQ=60)

      CTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCG                                                                                         <  SRR4302542.160221/94‑1 (MQ=60)

      CTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCG                                                                                         >  SRR4302542.5067766/1‑94 (MQ=60)

      CTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCG                                                                                         <  SRR4302542.2257704/94‑1 (MQ=60)

      CTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCG                                                                                         >  SRR4302542.6577686/1‑94 (MQ=60)

      CTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCG                                                                                         >  SRR4302542.5466121/1‑94 (MQ=60)

       TCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGC                                                                                        >  SRR4302542.6022868/1‑94 (MQ=60)

        CTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCC                                                                                       >  SRR4302542.7333262/1‑94 (MQ=60)

        CTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCC                                                                                       >  SRR4302542.7513868/1‑94 (MQ=60)

          TTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGG                                                                                     >  SRR4302542.4477428/1‑94 (MQ=60)

          TTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGG                                                                                     <  SRR4302542.6346488/94‑1 (MQ=60)

            TACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTA                                                                                   >  SRR4302542.1280183/1‑94 (MQ=60)

              CCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACA                                                                                 <  SRR4302542.3670420/94‑1 (MQ=60)

              CCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACA                                                                                 >  SRR4302542.6820045/1‑94 (MQ=60)

               CGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAG                                                                                <  SRR4302542.4451295/94‑1 (MQ=60)

               CGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAG                                                                                <  SRR4302542.1091258/94‑1 (MQ=60)

               CGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAG                                                                                >  SRR4302542.6632165/1‑94 (MQ=60)

                 GTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGAT                                                                              <  SRR4302542.3394254/94‑1 (MQ=60)

                    CGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCC                                                                           <  SRR4302542.6006485/94‑1 (MQ=60)

                     GCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCA                                                                          <  SRR4302542.6875798/94‑1 (MQ=60)

                      CGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCAT                                                                         <  SRR4302542.2692139/94‑1 (MQ=60)

                       GATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATG                                                                        >  SRR4302542.4297486/1‑94 (MQ=60)

                          CCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACA                                                                     >  SRR4302542.4674259/1‑94 (MQ=60)

                          CCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACA                                                                     >  SRR4302542.2414677/1‑94 (MQ=60)

                          CCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACA                                                                     <  SRR4302542.4086107/94‑1 (MQ=60)

                           CCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAA                                                                    >  SRR4302542.1950708/1‑94 (MQ=60)

                           CCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAA                                                                    >  SRR4302542.6663124/1‑94 (MQ=60)

                            CAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAAT                                                                   >  SRR4302542.6538920/1‑94 (MQ=60)

                            CAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAAT                                                                   <  SRR4302542.6779364/94‑1 (MQ=60)

                            CAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAAT                                                                   >  SRR4302542.3679569/1‑94 (MQ=60)

                             AGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATA                                                                  >  SRR4302542.2398084/1‑94 (MQ=60)

                             AGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATA                                                                  <  SRR4302542.5229163/94‑1 (MQ=60)

                             AGATACCGTTACTGTTCGCATCCAACTGCACAATGACACAGTCTCCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATA                                                                  <  SRR4302542.627396/94‑1 (MQ=60)

                              GATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAA                                                                 >  SRR4302542.7548555/1‑94 (MQ=60)

                               ATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAAC                                                                >  SRR4302542.5584902/1‑94 (MQ=60)

                                TACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACC                                                               >  SRR4302542.2883844/1‑94 (MQ=60)

                                TACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACC                                                               <  SRR4302542.5013099/94‑1 (MQ=60)

                                TACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACC                                                               >  SRR4302542.3402038/1‑94 (MQ=60)

                                TACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACC                                                               <  SRR4302542.5761953/94‑1 (MQ=60)

                                 tctttccctacacgacgctcttccgatctgagagttGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCC                                                              <  SRR4302542.1597736/58‑1 (MQ=60)

                                 ACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCC                                                              <  SRR4302542.6528933/94‑1 (MQ=60)

                                 ACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCC                                                              >  SRR4302542.3265289/1‑94 (MQ=60)

                                 ACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCC                                                              <  SRR4302542.2574571/94‑1 (MQ=60)

                                 ACCGTTACTGTTCGCATCCAACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCC                                                              >  SRR4302542.4952586/1‑94 (MQ=60)

                                  CCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCG                                                             <  SRR4302542.1965146/94‑1 (MQ=60)

                                   CGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGC                                                            <  SRR4302542.5546292/94‑1 (MQ=60)

                                   CGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGC                                                            >  SRR4302542.792221/1‑94 (MQ=60)

                                    GTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCC                                                           >  SRR4302542.5356265/1‑94 (MQ=60)

                                    GTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCC                                                           >  SRR4302542.6184860/1‑94 (MQ=60)

                                    GTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCC                                                           >  SRR4302542.6962041/1‑94 (MQ=60)

                                    GTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCC                                                           >  SRR4302542.2048565/1‑94 (MQ=60)

                                      TACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCT                                                         >  SRR4302542.4424626/1‑94 (MQ=60)

                                      TACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCT                                                         <  SRR4302542.2496214/94‑1 (MQ=60)

                                      TACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCT                                                         <  SRR4302542.5689225/94‑1 (MQ=60)

                                      TACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCT                                                         >  SRR4302542.6697113/1‑94 (MQ=60)

                                        CTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCT                                                       <  SRR4302542.4008655/94‑1 (MQ=60)

                                        CTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCT                                                       <  SRR4302542.3502209/94‑1 (MQ=60)

                                         TGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTG                                                      <  SRR4302542.3694813/94‑1 (MQ=60)

                                          GTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGG                                                     <  SRR4302542.6706864/94‑1 (MQ=60)

                                          GTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGG                                                     >  SRR4302542.6016434/1‑94 (MQ=60)

                                          GTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGG                                                     <  SRR4302542.1882328/94‑1 (MQ=60)

                                           TTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGA                                                    >  SRR4302542.4608447/1‑94 (MQ=60)

                                            TCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCTGGTACAGATGCCATGACAATAACCCGCCCTCTGGAG                                                   >  SRR4302542.4762314/1‑94 (MQ=60)

                                            TCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAG                                                   >  SRR4302542.4039549/1‑94 (MQ=60)

                                              GCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGAT                                                 <  SRR4302542.2512375/94‑1 (MQ=60)

                                              GCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGAT                                                 >  SRR4302542.4989521/1‑94 (MQ=60)

                                              GCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGAT                                                 <  SRR4302542.5265697/94‑1 (MQ=60)

                                              GCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGAT                                                 <  SRR4302542.4840182/94‑1 (MQ=60)

                                               CATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATG                                                >  SRR4302542.4477518/1‑94 (MQ=60)

                                               CATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATG                                                >  SRR4302542.556658/1‑94 (MQ=60)

                                                ATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGC                                               >  SRR4302542.3642925/1‑94 (MQ=60)

                                                 TCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCT                                              >  SRR4302542.1175458/1‑94 (MQ=60)

                                                  CCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTT                                             <  SRR4302542.3791596/94‑1 (MQ=60)

                                                  CCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTT                                             <  SRR4302542.3453587/94‑1 (MQ=60)

                                                   CCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTC                                            <  SRR4302542.116825/94‑1 (MQ=60)

                                                   CCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTC                                            <  SRR4302542.1768117/94‑1 (MQ=60)

                                                   CCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTC                                            <  SRR4302542.4828463/94‑1 (MQ=60)

                                                    CACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCG                                           <  SRR4302542.5250811/94‑1 (MQ=60)

                                                     ACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGC                                          <  SRR4302542.2362411/94‑1 (MQ=60)

                                                     ACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGC                                          >  SRR4302542.6844011/1‑94 (MQ=60)

                                                     ACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGC                                          <  SRR4302542.2320880/94‑1 (MQ=60)

                                                     ACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGC                                          >  SRR4302542.3223942/1‑94 (MQ=60)

                                                      CTGCCCAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCG                                         <  SRR4302542.3829532/94‑1 (MQ=60)

                                                       TGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGA                                        <  SRR4302542.4264778/94‑1 (MQ=60)

                                                        GCACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGAC                                       >  SRR4302542.1432704/1‑94 (MQ=60)

                                                         CACAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACG                                      >  SRR4302542.744524/1‑94 (MQ=60)

                                                          ACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGG                                     <  SRR4302542.3647399/94‑1 (MQ=60)

                                                           CAATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGT                                    >  SRR4302542.7013037/1‑94 (MQ=60)

                                                           CAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGT                                    >  SRR4302542.4397557/1‑94 (MQ=60)

                                                             ATGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAA                                  >  SRR4302542.4301306/1‑94 (MQ=60)

                                                              TGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAA                                 <  SRR4302542.7392182/94‑1 (MQ=60)

                                                              TGACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAA                                 <  SRR4302542.6170947/94‑1 (MQ=60)

                                                              TGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAA                                 <  SRR4302542.3934751/94‑1 (MQ=60)

                                                               GACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAT                                <  SRR4302542.5001513/94‑1 (MQ=60)

                                                               GACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAT                                <  SRR4302542.7101336/94‑1 (MQ=60)

                                                               GACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAT                                <  SRR4302542.3118016/94‑1 (MQ=60)

                                                               GACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAAT                                <  SRR4302542.6534577/94‑1 (MQ=60)

                                                                ACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGGCAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATA                               <  SRR4302542.3593762/94‑1 (MQ=60)

                                                                ACACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATA                               >  SRR4302542.1087011/1‑94 (MQ=60)

                                                                ACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATA                               >  SRR4302542.5421188/1‑94 (MQ=60)

                                                                 CACAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATAC                              <  SRR4302542.2336790/94‑1 (MQ=60)

                                                                  ACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACC                             <  SRR4302542.3789023/94‑1 (MQ=60)

                                                                   CAGTCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCC                            >  SRR4302542.4403960/1‑94 (MQ=60)

                                                                   CAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCC                            <  SRR4302542.3436868/94‑1 (MQ=60)

                                                                   CAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCC                            <  SRR4302542.590960/94‑1 (MQ=60)

                                                                     GTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGC                          >  SRR4302542.6490796/1‑94 (MQ=60)

                                                                      TCACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCA                         <  SRR4302542.3969415/94‑1 (MQ=60)

                                                                       CACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAG                        >  SRR4302542.4878319/1‑94 (MQ=60)

                                                                       CACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAG                        <  SRR4302542.2142391/94‑1 (MQ=60)

                                                                       CACCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAG                        >  SRR4302542.4841863/1‑94 (MQ=60)

                                                                         CCCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCTCCCTCTGGAGATGCTTCGCGACGGTAAATAACCGCAGCC                      >  SRR4302542.777767/1‑94 (MQ=60)

                                                                         CCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCC                      >  SRR4302542.1067948/1‑94 (MQ=60)

                                                                          CCTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCNN                     >  SRR4302542.1238330/1‑94 (MQ=60)

                                                                           CTGTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAG                    >  SRR4302542.2502028/1‑94 (MQ=60)

                                                                            TGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGA                   <  SRR4302542.3862946/94‑1 (MQ=60)

                                                                             GTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGAT                  <  SRR4302542.3360369/94‑1 (MQ=60)

                                                                             GTCCGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGAT                  >  SRR4302542.765400/1‑94 (MQ=60)

                                                                                CGACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTC               >  SRR4302542.7297141/1‑94 (MQ=60)

                                                                                 GACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCA              <  SRR4302542.7412978/94‑1 (MQ=60)

                                                                                 GACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCA              >  SRR4302542.4836599/1‑94 (MQ=60)

                                                                                 GACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCA              >  SRR4302542.2511250/1‑94 (MQ=60)

                                                                                 GACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCA              >  SRR4302542.2481490/1‑94 (MQ=60)

                                                                                  ACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCAT             >  SRR4302542.1779471/1‑94 (MQ=60)

                                                                                  ACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCAT             <  SRR4302542.3088167/94‑1 (MQ=60)

                                                                                  ACAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCAT             <  SRR4302542.4566260/94‑1 (MQ=60)

                                                                                   CAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATC            <  SRR4302542.1863353/94‑1 (MQ=60)

                                                                                   CAATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATC            <  SRR4302542.3553360/94‑1 (MQ=60)

                                                                                     ATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTT          >  SRR4302542.844658/1‑94 (MQ=60)

                                                                                     ATTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTT          >  SRR4302542.5207815/1‑94 (MQ=60)

                                                                                      TTTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTC         <  SRR4302542.5553762/94‑1 (MQ=60)

                                                                                       TTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCC        >  SRR4302542.2897356/1‑94 (MQ=60)

                                                                                       TTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCC        <  SRR4302542.6884950/94‑1 (MQ=60)

                                                                                       TTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCC        >  SRR4302542.4677051/1‑94 (MQ=60)

                                                                                       TTCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCC        <  SRR4302542.1264941/94‑1 (MQ=60)

                                                                                       TTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCC        <  SRR4302542.1709883/94‑1 (MQ=60)

                                                                                        TCCGGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCA       <  SRR4302542.3406663/94‑1 (MQ=60)

                                                                                        TCCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCA       <  SRR4302542.101039/94‑1 (MQ=60)

                                                                                         CCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAG      >  SRR4302542.6701526/1‑94 (MQ=60)

                                                                                         CCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAG      >  SRR4302542.3945137/1‑94 (MQ=60)

                                                                                         CCAGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAG      >  SRR4302542.1445741/1‑94 (MQ=60)

                                                                                         CCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAG      >  SRR4302542.4109917/1‑94 (MQ=60)

                                                                                           AGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCG    >  SRR4302542.7086433/1‑94 (MQ=60)

                                                                                           AGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCG    >  SRR4302542.4923226/1‑94 (MQ=60)

                                                                                           AGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCG    >  SRR4302542.3307847/1‑94 (MQ=60)

                                                                                           AGTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCG    >  SRR4302542.2656238/1‑94 (MQ=60)

                                                                                            GTCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGC   >  SRR4302542.1002364/1‑94 (MQ=60)

                                                                                             TCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTACCAGCGCC  >  SRR4302542.3198425/1‑94 (MQ=60)

GTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCC  >  NC_000913/2961418‑2961604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`