Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,963,770 T→C D97G (GAC→GGC)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,963,7700TC83.7% 549.7 / 93.9 253D97G (GAC→GGC) lgtphosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base C (87/124);  total (106/147)
Fisher's exact test for biased strand distribution p-value = 8.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACG  >  NC_000913/2963679‑2963859
                                                                                           |                                                                                         
 aCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGccc                                                                                         <  2:1115037/93‑1 (MQ=255)
   cTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCa                                                                                       <  2:2536400/93‑1 (MQ=255)
    tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAg                                                                                      <  1:6703604/93‑1 (MQ=255)
     ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCCCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGa                                                                                     <  1:3351070/93‑1 (MQ=255)
     ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCTGCCGTCCCAGa                                                                                     <  2:5279996/93‑1 (MQ=255)
       aagaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGaca                                                                                   <  1:3878225/93‑1 (MQ=255)
       aagaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGaca                                                                                   <  1:6927307/93‑1 (MQ=255)
        agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                  <  1:1178104/93‑1 (MQ=255)
         gaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACg                                                                                 <  1:1004790/93‑1 (MQ=255)
         gaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACg                                                                                 <  1:3666683/93‑1 (MQ=255)
           agGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGa                                                                               <  2:3942276/93‑1 (MQ=255)
            ggAACGTTTAGTACGGCGGGCGAAGAAAAAAATCACCACCAAAAAGCCAAACAAGCCGCCCCGGGAAGAAAAGCCGCCCCCCCCCACACGGaa                                                                              >  2:502541/1‑93 (MQ=255)
              aaCGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACa                                                                            <  1:4696332/93‑1 (MQ=255)
               aCGTTTAGTACGGCGGGCGAAGAAAAAAATCACCACGATAAAGCCAAACAGGCCGCCCCGGAAAGACAAGCCGCCGGCCCCGACACGGAACaa                                                                           >  2:2362962/1‑92 (MQ=255)
                cGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGa                                                                          <  2:5813672/93‑1 (MQ=255)
                cGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGa                                                                          <  2:4991104/93‑1 (MQ=255)
                cGTTTAGTACGGCGGGCGAAGAAAAACATCACCACCATAACGCCAATCAGGCCGCCGGGGGAAGACAAGCCGCCGCCCCCGACACGGAAAAGa                                                                          >  2:1535853/1‑93 (MQ=255)
                  tttAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATa                                                                        <  1:640929/93‑1 (MQ=255)
                   ttAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATAc                                                                       <  2:5344056/93‑1 (MQ=255)
                    tAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACa                                                                      <  2:6165767/93‑1 (MQ=255)
                     aGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAg                                                                     <  1:290970/93‑1 (MQ=255)
                     aGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAg                                                                     <  2:1355128/93‑1 (MQ=255)
                       tACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCg                                                                   <  2:352054/93‑1 (MQ=255)
                          ggcgggggAAGATAATTATCACCACGATAAAGCCAATCAGGCCGCCGGGGGAAGACATGGCGCCGCCCCCGACACCGAACAGATACAGCGGaa                                                                >  1:6083771/1‑92 (MQ=255)
                          ggcgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGAt                                                                <  1:4433391/93‑1 (MQ=255)
                            cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCg                                                              <  1:2219143/93‑1 (MQ=255)
                            cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCg                                                              <  1:803827/93‑1 (MQ=255)
                             gggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCgg                                                             >  1:4726212/1‑93 (MQ=255)
                               gcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGcc                                                           >  2:588535/1‑93 (MQ=255)
                                 gAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCAt                                                         >  1:3542435/1‑93 (MQ=255)
                                  aaGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATa                                                        <  1:5439926/93‑1 (MQ=255)
                                   aGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaa                                                       >  2:4060294/1‑93 (MQ=255)
                                    gATAATCATCACCGCGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                      <  1:3288118/93‑1 (MQ=255)
                                    gATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                      <  1:712267/93‑1 (MQ=255)
                                      tAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACt                                                    >  1:5512933/1‑93 (MQ=255)
                                      tAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACt                                                    >  2:6059798/1‑93 (MQ=255)
                                        atcatcACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc                                                  <  1:5004154/93‑1 (MQ=255)
                                        atcatcACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGc                                                  >  2:7186625/1‑93 (MQ=255)
                                         tcatcaCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCg                                                 <  1:384757/93‑1 (MQ=255)
                                          catcaCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCgg                                                <  2:4641513/93‑1 (MQ=255)
                                           atcaCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCggg                                               <  1:2317538/93‑1 (MQ=255)
                                             gaccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaa                                             >  2:7319130/2‑93 (MQ=255)
                                              accacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaaa                                            <  1:4193180/93‑1 (MQ=255)
                                               ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAt                                           >  1:4023961/1‑93 (MQ=255)
                                               ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAt                                           >  1:7113074/1‑93 (MQ=255)
                                               ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGCGAAAt                                           >  2:6791810/1‑93 (MQ=255)
                                                cacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAtt                                          <  2:2280449/93‑1 (MQ=255)
                                                 acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTg                                         >  1:2516509/1‑93 (MQ=255)
                                                 acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTg                                         <  1:156614/93‑1 (MQ=255)
                                                   gATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTa                                       <  1:6841523/93‑1 (MQ=255)
                                                     tAACGCGAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTaga                                     >  2:6987348/1‑93 (MQ=255)
                                                      aaCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaa                                    >  1:5170737/1‑93 (MQ=255)
                                                       aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                   <  2:6295990/93‑1 (MQ=255)
                                                       aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGGagaac                                   >  1:2059367/1‑93 (MQ=255)
                                                        cGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaaca                                  >  2:2212271/1‑93 (MQ=255)
                                                         gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacag                                 >  2:1885804/1‑93 (MQ=255)
                                                         gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacag                                 <  2:2019256/93‑1 (MQ=255)
                                                          ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacaga                                >  2:1378291/1‑93 (MQ=255)
                                                          ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacaga                                <  2:4348859/93‑1 (MQ=255)
                                                           cAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaa                               >  2:4365862/1‑93 (MQ=255)
                                                           cAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaa                               <  2:2909956/93‑1 (MQ=255)
                                                            aaTCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                              >  2:3782234/1‑93 (MQ=255)
                                                            aaTCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                              >  2:838543/1‑93 (MQ=255)
                                                            aaTCAGGCCGCCGTGGAAAGACATGCCACCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                              >  1:3310918/1‑93 (MQ=255)
                                                             aTCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAgaacagaaca                             <  1:5642905/93‑1 (MQ=255)
                                                              tCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAt                            >  2:3213702/1‑93 (MQ=255)
                                                              tCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAt                            <  1:1949668/93‑1 (MQ=255)
                                                               cAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATa                           <  2:127937/93‑1 (MQ=255)
                                                               cAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATa                           >  2:4615650/1‑93 (MQ=255)
                                                                aGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATaa                          >  1:3000867/1‑93 (MQ=255)
                                                                aGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATaa                          <  2:6915642/93‑1 (MQ=255)
                                                                aGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATaa                          >  2:5406041/1‑93 (MQ=255)
                                                                aGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATaa                          <  1:6526015/93‑1 (MQ=255)
                                                                 ggCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAc                         >  2:1713707/1‑93 (MQ=255)
                                                                   ccgccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCa                       >  2:4714714/1‑93 (MQ=255)
                                                                    cgccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCaa                      <  2:2052475/93‑1 (MQ=255)
                                                                     gccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAAt                     >  2:2649203/1‑93 (MQ=255)
                                                                     gccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTACGGGAAATTGTAGAACAGAACATAACCAAt                     <  2:19369/93‑1 (MQ=255)
                                                                      ccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATa                    <  1:2782007/93‑1 (MQ=255)
                                                                        gTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACg                  >  1:944856/1‑93 (MQ=255)
                                                                        gTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACg                  >  2:1667334/1‑93 (MQ=255)
                                                                         tGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGt                 <  1:5925607/93‑1 (MQ=255)
                                                                         tGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACgc                 >  1:3166383/1‑92 (MQ=255)
                                                                          ggAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTc                >  1:425915/1‑93 (MQ=255)
                                                                           gAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcc               <  2:6582334/93‑1 (MQ=255)
                                                                           gAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcc               <  1:6374583/93‑1 (MQ=255)
                                                                           gAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcc               <  1:2505728/93‑1 (MQ=255)
                                                                             aaGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcccc             >  1:324411/1‑93 (MQ=255)
                                                                             aaGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcccc             >  2:6515872/1‑93 (MQ=255)
                                                                             aaGACATGCCCCCCCCCCAGACAACGAAAAAATAAAGGGGATTGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTcccc             <  1:5600225/93‑1 (MQ=255)
                                                                                aCATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGa          <  2:7489416/93‑1 (MQ=255)
                                                                                 naTGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCgnn         >  2:3462762/2‑91 (MQ=255)
                                                                                 cATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAg         <  1:1414532/93‑1 (MQ=255)
                                                                                 cATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAg         <  2:1999133/93‑1 (MQ=255)
                                                                                   tGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGa       >  1:742222/1‑93 (MQ=255)
                                                                                   tGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGa       <  2:248198/93‑1 (MQ=255)
                                                                                    gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGaa      <  2:2899523/93‑1 (MQ=255)
                                                                                       gccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGAc   <  2:5744545/93‑1 (MQ=255)
                                                                                       gccgTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGAc   <  1:7329485/93‑1 (MQ=255)
                                                                                        ccgCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACg  >  1:3484967/1‑93 (MQ=255)
                                                                                           |                                                                                         
GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACG  >  NC_000913/2963679‑2963859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGA  >  NC_000913/2963679‑2963863
                                                                                           |                                                                                             
GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCC                                                                                             <  SRR4302542.7542312/94‑1 (MQ=60)
GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCC                                                                                             <  SRR4302542.4481602/94‑1 (MQ=60)
    TGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGA                                                                                         <  SRR4302542.6769652/94‑1 (MQ=60)
     GGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCCCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGAC                                                                                        <  SRR4302542.3382598/94‑1 (MQ=60)
       AAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACAC                                                                                      <  SRR4302542.7254514/94‑1 (MQ=60)
       AAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACAC                                                                                      <  SRR4302542.3916532/94‑1 (MQ=60)
       AAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACAC                                                                                      <  SRR4302542.6995320/94‑1 (MQ=60)
        AGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACG                                                                                     <  SRR4302542.1188281/94‑1 (MQ=60)
         GAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGG                                                                                    <  SRR4302542.1013443/94‑1 (MQ=60)
         GAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGG                                                                                    <  SRR4302542.3701625/94‑1 (MQ=60)
           AGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAA                                                                                  <  SRR4302542.6170731/94‑1 (MQ=60)
            GGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAAC                                                                                 <  SRR4302542.4311011/94‑1 (MQ=60)
              AACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAG                                                                               <  SRR4302542.4742180/94‑1 (MQ=60)
                CGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGAT                                                                             <  SRR4302542.6971009/94‑1 (MQ=60)
                CGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGAT                                                                             <  SRR4302542.6586795/94‑1 (MQ=60)
                 GTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATA                                                                            <  SRR4302542.4037562/94‑1 (MQ=60)
                  TTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATAC                                                                           <  SRR4302542.3125803/94‑1 (MQ=60)
                  TTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATAC                                                                           <  SRR4302542.748633/94‑1 (MQ=60)
                  TTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATAC                                                                           <  SRR4302542.646443/94‑1 (MQ=60)
                   TTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACA                                                                          <  SRR4302542.4556898/94‑1 (MQ=60)
                   TTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACA                                                                          <  SRR4302542.5883561/94‑1 (MQ=60)
                    TAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAG                                                                         <  SRR4302542.5492402/94‑1 (MQ=60)
                     AGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGC                                                                        <  SRR4302542.293512/94‑1 (MQ=60)
                      GTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCG                                                                       <  SRR4302542.2272963/94‑1 (MQ=60)
                        ACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGA                                                                     <  SRR4302542.207220/94‑1 (MQ=60)
                         CGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACGGATACAGCGGAT                                                                    <  SRR4302542.339310/94‑1 (MQ=60)
                          GGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATC                                                                   <  SRR4302542.4476913/94‑1 (MQ=60)
                          GGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATC                                                                   <  SRR4302542.6526850/94‑1 (MQ=60)
                            CGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGG                                                                 <  SRR4302542.2238235/94‑1 (MQ=60)
                            CGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGG                                                                 <  SRR4302542.810739/94‑1 (MQ=60)
                            CGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGG                                                                 >  SRR4302542.4772270/1‑94 (MQ=60)
                            CGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGG                                                                 <  SRR4302542.4275449/94‑1 (MQ=60)
                                CGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCAT                                                             >  SRR4302542.3575526/1‑94 (MQ=60)
                                CGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCAT                                                             <  SRR4302542.6810768/94‑1 (MQ=60)
                                 GAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATA                                                            >  SRR4302542.3734692/1‑94 (MQ=60)
                                 GAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATA                                                            >  SRR4302542.2709203/1‑94 (MQ=60)
                                  AAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAA                                                           <  SRR4302542.5492778/94‑1 (MQ=60)
                                  AAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAA                                                           >  SRR4302542.3204665/1‑94 (MQ=60)
                                   AGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAA                                                          >  SRR4302542.2788342/1‑94 (MQ=60)
                                    GATAATCATCACCGCGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAAC                                                         <  SRR4302542.3319165/94‑1 (MQ=60)
                                    GATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAAC                                                         <  SRR4302542.718412/94‑1 (MQ=60)
                                     ATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACT                                                        >  SRR4302542.5566504/1‑94 (MQ=60)
                                     ATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACT                                                        >  SRR4302542.3337409/1‑94 (MQ=60)
                                      TAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTG                                                       <  SRR4302542.553128/94‑1 (MQ=60)
                                      TAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTG                                                       <  SRR4302542.3945259/94‑1 (MQ=60)
                                       AATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATCCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGC                                                      >  SRR4302542.3579178/1‑94 (MQ=60)
                                        ATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCG                                                     <  SRR4302542.5052757/94‑1 (MQ=60)
                                        ATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCG                                                     >  SRR4302542.4463158/1‑94 (MQ=60)
                                         TCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG                                                    <  SRR4302542.388097/94‑1 (MQ=60)
                                         TCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG                                                    >  SRR4302542.3283749/1‑94 (MQ=60)
                                          CATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGTCATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGG                                                   >  SRR4302542.2455606/1‑94 (MQ=60)
                                           ATCCCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGA                                                  <  SRR4302542.389983/94‑1 (MQ=60)
                                           ATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGA                                                  >  SRR4302542.2939038/1‑94 (MQ=60)
                                           ATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGA                                                  >  SRR4302542.5529307/1‑94 (MQ=60)
                                           ATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGA                                                  >  SRR4302542.5663196/1‑94 (MQ=60)
                                           ATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGA                                                  <  SRR4302542.2337416/94‑1 (MQ=60)
                                             CACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTACGGGAAA                                                >  SRR4302542.4922563/1‑94 (MQ=60)
                                              ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAT                                               <  SRR4302542.7564518/94‑1 (MQ=60)
                                              ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAT                                               <  SRR4302542.1794273/94‑1 (MQ=60)
                                              ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAT                                               >  SRR4302542.7182895/1‑94 (MQ=60)
                                              ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAT                                               >  SRR4302542.4063662/1‑94 (MQ=60)
                                              ACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAAT                                               <  SRR4302542.4234524/94‑1 (MQ=60)
                                               CCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATT                                              >  SRR4302542.1056324/1‑94 (MQ=60)
                                                CACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTG                                             >  SRR4302542.2540922/1‑94 (MQ=60)
                                                 ACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGT                                            >  SRR4302542.6630512/1‑94 (MQ=60)
                                                 ACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGT                                            <  SRR4302542.3101670/94‑1 (MQ=60)
                                                 ACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGT                                            <  SRR4302542.157944/94‑1 (MQ=60)
                                                  CGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTA                                           >  SRR4302542.6652279/1‑94 (MQ=60)
                                                   GATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAG                                          <  SRR4302542.6908735/94‑1 (MQ=60)
                                                     TAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAA                                        >  SRR4302542.5220990/1‑94 (MQ=60)
                                                      AACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAAC                                       <  SRR4302542.3356873/94‑1 (MQ=60)
                                                      AACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAAC                                       <  SRR4302542.3319851/94‑1 (MQ=60)
                                                      AACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGGAGAAC                                       >  SRR4302542.2077163/1‑94 (MQ=60)
                                                       ACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACA                                      <  SRR4302542.5461756/94‑1 (MQ=60)
                                                        CGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAG                                     <  SRR4302542.7213166/94‑1 (MQ=60)
                                                         gccaatccagccgcctctgaacgcccTGCCGCCGTCCCAGACACGGACCAGATACCGCGGATCGGTCCTAAACTGCGGGAcaccgcagaacacc                                    >  SRR4302542.5748900/27‑80 (MQ=38)
                                                         GCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGA                                    >  SRR4302542.3644018/1‑94 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   <  SRR4302542.5132429/94‑1 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   <  SRR4302542.6962827/94‑1 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   >  SRR4302542.7002376/1‑94 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   <  SRR4302542.5572185/94‑1 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   <  SRR4302542.300646/94‑1 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   <  SRR4302542.230864/94‑1 (MQ=60)
                                                          CCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAA                                   >  SRR4302542.7055645/1‑94 (MQ=60)
                                                           CAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAAC                                  <  SRR4302542.4228530/94‑1 (MQ=60)
                                                           CAATCAGGCCGCCGTGGAAAGACATGCCACCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAAC                                  >  SRR4302542.3342129/1‑94 (MQ=60)
                                                             ATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAT                                <  SRR4302542.5697698/94‑1 (MQ=60)
                                                             ATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAT                                >  SRR4302542.4766548/1‑94 (MQ=60)
                                                              TCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATA                               <  SRR4302542.1966530/94‑1 (MQ=60)
                                                               CAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAA                              >  SRR4302542.3029569/1‑94 (MQ=60)
                                                               CAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAA                              >  SRR4302542.4976130/1‑94 (MQ=60)
                                                               CAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAA                              >  SRR4302542.5664338/1‑94 (MQ=60)
                                                                AGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAC                             <  SRR4302542.6590326/94‑1 (MQ=60)
                                                                AGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAC                             <  SRR4302542.1754178/94‑1 (MQ=60)
                                                                   CCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAA                          <  SRR4302542.2935144/94‑1 (MQ=60)
                                                                    CGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAAT                         <  SRR4302542.4225794/94‑1 (MQ=60)
                                                                    CGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAAT                         >  SRR4302542.6283630/1‑94 (MQ=60)
                                                                      CCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATAC                       <  SRR4302542.7184520/94‑1 (MQ=60)
                                                                      CCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATAC                       >  SRR4302542.3856754/1‑94 (MQ=60)
                                                                      CCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATAC                       <  SRR4302542.2808792/94‑1 (MQ=60)
                                                                       CGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACG                      <  SRR4302542.810939/94‑1 (MQ=60)
                                                                       CGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACG                      >  SRR4302542.952986/1‑94 (MQ=60)
                                                                        GTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGT                     >  SRR4302542.3517763/1‑94 (MQ=60)
                                                                        GTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGT                     >  SRR4302542.1407414/1‑94 (MQ=60)
                                                                        GTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGC                     >  SRR4302542.3196422/1‑94 (MQ=60)
                                                                         TGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTC                    <  SRR4302542.5983027/94‑1 (MQ=60)
                                                                         TGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTC                    >  SRR4302542.429596/1‑94 (MQ=60)
                                                                         TGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTC                    <  SRR4302542.218493/94‑1 (MQ=60)
                                                                         TGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTC                    <  SRR4302542.6047496/94‑1 (MQ=60)
                                                                           GAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCC                  <  SRR4302542.6437351/94‑1 (MQ=60)
                                                                           GAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCC                  <  SRR4302542.1472408/94‑1 (MQ=60)
                                                                           GAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCC                  <  SRR4302542.2530050/94‑1 (MQ=60)
                                                                            AAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCC                 >  SRR4302542.327226/1‑94 (MQ=60)
                                                                              AGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCG               <  SRR4302542.4571819/94‑1 (MQ=60)
                                                                                ACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAG             <  SRR4302542.1374034/94‑1 (MQ=60)
                                                                                 CATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGG            <  SRR4302542.1426759/94‑1 (MQ=60)
                                                                                 CATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGG            >  SRR4302542.2338442/1‑94 (MQ=60)
                                                                                  ATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGA           >  SRR4302542.3311707/1‑94 (MQ=60)
                                                                                  ATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGA           >  SRR4302542.748659/1‑94 (MQ=60)
                                                                                    GCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAG         <  SRR4302542.6444613/94‑1 (MQ=60)
                                                                                      CGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGAC       <  SRR4302542.825497/94‑1 (MQ=60)
                                                                                       GCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACG      <  SRR4302542.7401290/94‑1 (MQ=60)
                                                                                       GCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACG      >  SRR4302542.2537041/1‑94 (MQ=60)
                                                                                       GCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACG      >  SRR4302542.3517569/1‑94 (MQ=60)
                                                                                        CCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGC     >  SRR4302542.2791115/1‑94 (MQ=60)
                                                                                         CGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCC    <  SRR4302542.1751043/94‑1 (MQ=60)
                                                                                          GCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCG   <  SRR4302542.2093034/94‑1 (MQ=60)
                                                                                           CCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGA  >  SRR4302542.7508210/1‑94 (MQ=60)
                                                                                           CCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGA  <  SRR4302542.5668227/94‑1 (MQ=60)
                                                                                           CCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGA  >  SRR4302542.7024419/1‑94 (MQ=60)
                                                                                           |                                                                                             
GACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGA  >  NC_000913/2963679‑2963863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑