Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,985,029 G→A W387* (TGG→TGA)  b2845 → putative transporter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,985,0290GA87.6% 782.4 / 65.1 267W387* (TGG→TGA) b2845putative transporter protein
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base A (120/114);  total (138/129)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

Only 100 of 269 total aligned reads displayed.

ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAA  >  NC_000913/2984937‑2985120
                                                                                            |                                                                                           
  gatTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCCGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACg                                                                                           >  2:3659743/1‑93 (MQ=255)
    tttACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGtt                                                                                         >  1:7463204/1‑93 (MQ=255)
       aCGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCa                                                                                      >  1:6792325/1‑93 (MQ=255)
       aCGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCa                                                                                      <  1:4841653/93‑1 (MQ=255)
            aTCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGa                                                                                 >  2:2072467/1‑93 (MQ=255)
             tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGaa                                                                                <  2:7076463/93‑1 (MQ=255)
             tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGaa                                                                                <  2:295675/93‑1 (MQ=255)
             tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGaa                                                                                <  2:5678775/93‑1 (MQ=255)
             tCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGaa                                                                                >  1:3114982/1‑93 (MQ=255)
              cAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGaaa                                                                               <  2:469488/93‑1 (MQ=255)
                gCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATc                                                                             <  1:5251011/93‑1 (MQ=255)
                   gCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGa                                                                          >  2:7169556/1‑93 (MQ=255)
                   gCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGa                                                                          >  1:2587564/1‑93 (MQ=255)
                   gCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGa                                                                          >  2:5268447/1‑93 (MQ=255)
                    cccGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAt                                                                         <  1:1026179/93‑1 (MQ=255)
                    cccGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAt                                                                         <  1:7106616/93‑1 (MQ=255)
                    cccGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAt                                                                         <  1:22187/93‑1 (MQ=255)
                     ccGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAtt                                                                        >  2:1533345/1‑93 (MQ=255)
                      cGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTa                                                                       >  1:1617078/1‑93 (MQ=255)
                       gCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTAc                                                                      <  1:7273158/93‑1 (MQ=255)
                       gCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTAc                                                                      <  1:5792616/93‑1 (MQ=255)
                        cTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACg                                                                     >  2:2794773/1‑93 (MQ=255)
                        cTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACg                                                                     >  2:6762743/1‑93 (MQ=255)
                         tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGc                                                                    >  1:7160802/1‑93 (MQ=255)
                         tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGc                                                                    >  1:945290/1‑93 (MQ=255)
                         tCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGc                                                                    >  2:5136546/1‑93 (MQ=255)
                          cATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCt                                                                   <  1:564910/93‑1 (MQ=255)
                          cATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCt                                                                   <  1:3595074/93‑1 (MQ=255)
                           aTTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTg                                                                  >  1:4067972/1‑93 (MQ=255)
                            ttGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGa                                                                 >  1:1480141/1‑93 (MQ=255)
                            ttGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGa                                                                 >  2:4721035/1‑93 (MQ=255)
                              gCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATc                                                               <  2:646261/93‑1 (MQ=255)
                                cATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGt                                                             >  2:2684819/1‑93 (MQ=255)
                                cATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGt                                                             <  2:1366536/93‑1 (MQ=255)
                                  tGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTgg                                                           <  1:2271563/93‑1 (MQ=255)
                                  tGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTgg                                                           >  1:247408/1‑93 (MQ=255)
                                  tGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCCTgg                                                           >  2:6246329/1‑93 (MQ=255)
                                   gATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTggg                                                          >  2:6701316/1‑93 (MQ=255)
                                   gATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTggg                                                          >  1:6073346/1‑93 (MQ=255)
                                    aTACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGt                                                         >  1:7025770/1‑93 (MQ=255)
                                     tACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTa                                                        >  2:4226902/1‑93 (MQ=255)
                                       cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATc                                                      >  1:4213011/1‑93 (MQ=255)
                                       cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATc                                                      >  2:5721408/1‑93 (MQ=255)
                                       cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATc                                                      >  2:1829377/1‑93 (MQ=255)
                                       cTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCCGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATc                                                      <  1:3260170/93‑1 (MQ=255)
                                        ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGGGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATcc                                                     >  1:6219440/1‑93 (MQ=255)
                                        ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATcc                                                     <  1:1723950/93‑1 (MQ=255)
                                         tttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCt                                                    <  1:3366712/93‑1 (MQ=255)
                                         tttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCt                                                    >  1:5095787/1‑93 (MQ=255)
                                             atcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGc                                                >  2:5925569/1‑93 (MQ=255)
                                              tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCg                                               >  1:2820818/1‑93 (MQ=255)
                                               catGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGt                                              >  2:4960952/1‑93 (MQ=255)
                                                 tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTAt                                            >  2:1607275/1‑93 (MQ=255)
                                                 tGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTAt                                            >  1:1342685/1‑93 (MQ=255)
                                                  gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATc                                           <  2:397114/93‑1 (MQ=255)
                                                    cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCgg                                         >  2:7260481/1‑93 (MQ=255)
                                                     tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGt                                        <  1:3833480/93‑1 (MQ=255)
                                                      aCGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTa                                       >  2:5586522/1‑93 (MQ=255)
                                                       cGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTaa                                      >  1:6886609/1‑93 (MQ=255)
                                                        gCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAAt                                     <  2:6498719/93‑1 (MQ=255)
                                                        gCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAAt                                     <  2:429458/93‑1 (MQ=255)
                                                         cTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATg                                    <  2:1911407/93‑1 (MQ=255)
                                                         cTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATg                                    >  2:2274735/1‑93 (MQ=255)
                                                          tGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGt                                   >  2:4171296/1‑93 (MQ=255)
                                                          tGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGt                                   <  2:5100485/93‑1 (MQ=255)
                                                            tCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTc                                 >  1:1853208/1‑93 (MQ=255)
                                                             cAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCt                                <  2:1122030/93‑1 (MQ=255)
                                                              aaCGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCtt                               <  1:1113758/93‑1 (MQ=255)
                                                               aCGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCttt                              <  1:6669165/93‑1 (MQ=255)
                                                                cGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTa                             <  2:3138097/93‑1 (MQ=255)
                                                                  tATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGc                           >  2:3590761/1‑93 (MQ=255)
                                                                    tctcATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTa                         >  1:5489530/1‑93 (MQ=255)
                                                                     ctcATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTaa                        <  2:7505755/93‑1 (MQ=255)
                                                                     ctcATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTaa                        >  1:4107738/1‑93 (MQ=255)
                                                                      tcATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAat                       >  2:6128397/1‑93 (MQ=255)
                                                                       cATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAata                      <  2:2470880/93‑1 (MQ=255)
                                                                       cATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAata                      <  1:6256375/93‑1 (MQ=255)
                                                                        aTCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAatat                     >  2:656520/1‑93 (MQ=255)
                                                                         tCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATc                    <  2:2670748/93‑1 (MQ=255)
                                                                         tCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATc                    <  1:452616/93‑1 (MQ=255)
                                                                         tCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATc                    <  1:2894994/93‑1 (MQ=255)
                                                                            cGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGt                 <  2:6649552/93‑1 (MQ=255)
                                                                            cGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGt                 >  1:6001698/1‑93 (MQ=255)
                                                                            cGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGt                 <  1:1661946/93‑1 (MQ=255)
                                                                            cGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGt                 <  1:4022152/93‑1 (MQ=255)
                                                                             ggCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGtt                >  2:2161183/1‑93 (MQ=255)
                                                                              gcgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTtg               >  1:7320574/1‑93 (MQ=255)
                                                                              gcgcTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTtg               <  2:4407011/93‑1 (MQ=255)
                                                                                gcTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTtgtg             <  2:341487/93‑1 (MQ=255)
                                                                                 cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTgg            <  1:3771611/93‑1 (MQ=255)
                                                                                  ttAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGa           >  1:4684115/1‑93 (MQ=255)
                                                                                  ttAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGa           <  1:6560510/93‑1 (MQ=255)
                                                                                  ttAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGa           <  1:2355627/93‑1 (MQ=255)
                                                                                   tAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGAt          <  2:6164483/93‑1 (MQ=255)
                                                                                   tAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGAt          <  2:1244111/93‑1 (MQ=255)
                                                                                    aaaCCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATg         <  1:649718/93‑1 (MQ=255)
                                                                                        ccTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTc     >  1:2114267/1‑93 (MQ=255)
                                                                                        ccTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTc     <  1:1202953/93‑1 (MQ=255)
                                                                                         cTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCa    >  2:1798735/1‑93 (MQ=255)
                                                                                          tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCaa   >  1:2170667/1‑93 (MQ=255)
                                                                                            |                                                                                           
ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAA  >  NC_000913/2984937‑2985120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAAT  >  NC_000913/2984936‑2985122
                                                                                             |                                                                                             
GATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGG                                                                                               <  SRR4302542.2437955/94‑1 (MQ=60)
GATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGG                                                                                               >  SRR4302542.4870013/1‑94 (MQ=60)
 ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGAC                                                                                              <  SRR4302542.2474348/94‑1 (MQ=60)
 ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGAC                                                                                              >  SRR4302542.2206550/1‑94 (MQ=60)
 ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGAC                                                                                              >  SRR4302542.2028863/1‑94 (MQ=60)
 ATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGAC                                                                                              <  SRR4302542.1558375/94‑1 (MQ=60)
  TGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACG                                                                                             <  SRR4302542.4935049/94‑1 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           >  SRR4302542.2704316/1‑94 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           >  SRR4302542.5252333/1‑94 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           <  SRR4302542.4280633/94‑1 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           >  SRR4302542.5041952/1‑94 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           >  SRR4302542.7536320/1‑94 (MQ=60)
    ATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTT                                                                                           >  SRR4302542.6146300/1‑94 (MQ=60)
       TACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAGCCCTGACGTTCCA                                                                                        <  SRR4302542.5801781/94‑1 (MQ=60)
       TACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCA                                                                                        >  SRR4302542.6859117/1‑94 (MQ=60)
        ACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCAT                                                                                       <  SRR4302542.4888809/94‑1 (MQ=60)
         CGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATC                                                                                      >  SRR4302542.657336/1‑94 (MQ=60)
           CGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGG                                                                                    >  SRR4302542.2536005/1‑94 (MQ=60)
             ATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAA                                                                                  >  SRR4302542.3144582/1‑94 (MQ=60)
             ATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAA                                                                                  <  SRR4302542.6369100/94‑1 (MQ=60)
              TCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAA                                                                                 <  SRR4302542.1328570/94‑1 (MQ=60)
               CAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAAT                                                                                >  SRR4302542.2226797/1‑94 (MQ=60)
                AGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATC                                                                               <  SRR4302542.7363311/94‑1 (MQ=60)
                AGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATC                                                                               >  SRR4302542.1456061/1‑94 (MQ=60)
                 GCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCT                                                                              <  SRR4302542.5302052/94‑1 (MQ=60)
                   GGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGA                                                                            >  SRR4302542.2612636/1‑94 (MQ=60)
                    GCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAT                                                                           <  SRR4302542.186589/94‑1 (MQ=60)
                    GCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGAT                                                                           >  SRR4302542.4101427/1‑94 (MQ=60)
                     CCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATT                                                                          <  SRR4302542.1035024/94‑1 (MQ=60)
                     CCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATT                                                                          <  SRR4302542.22380/94‑1 (MQ=60)
                     CCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATT                                                                          <  SRR4302542.1962884/94‑1 (MQ=60)
                     CCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATT                                                                          <  SRR4302542.7176370/94‑1 (MQ=60)
                      CCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTA                                                                         >  SRR4302542.2237878/1‑94 (MQ=60)
                      CCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTA                                                                         >  SRR4302542.1631046/1‑94 (MQ=60)
                       CGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTAC                                                                        <  SRR4302542.2211322/94‑1 (MQ=60)
                       CGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTAC                                                                        >  SRR4302542.129310/1‑94 (MQ=60)
                        GCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACG                                                                       <  SRR4302542.7344450/94‑1 (MQ=60)
                        GCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACG                                                                       <  SRR4302542.5848849/94‑1 (MQ=60)
                         CTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGC                                                                      >  SRR4302542.2405030/1‑94 (MQ=60)
                         CTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGC                                                                      >  SRR4302542.953422/1‑94 (MQ=60)
                         CTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGC                                                                      >  SRR4302542.7231044/1‑94 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTG                                                                    <  SRR4302542.569771/94‑1 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTG                                                                    <  SRR4302542.3628603/94‑1 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTG                                                                    <  SRR4302542.7282258/94‑1 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTG                                                                    >  SRR4302542.4108087/1‑94 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTG                                                                    <  SRR4302542.1806312/94‑1 (MQ=60)
                           CATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTG                                                                    >  SRR4302542.4110480/1‑94 (MQ=60)
                            ATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGA                                                                   >  SRR4302542.6530926/1‑94 (MQ=60)
                            ATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGA                                                                   >  SRR4302542.1127006/1‑94 (MQ=60)
                            ATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGA                                                                   >  SRR4302542.1492915/1‑94 (MQ=60)
                              TGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATC                                                                 >  SRR4302542.2812273/1‑94 (MQ=60)
                                CCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGT                                                               >  SRR4302542.5247836/1‑94 (MQ=60)
                                CCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGT                                                               <  SRR4302542.6256699/94‑1 (MQ=60)
                                 CATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTG                                                              >  SRR4302542.3393539/1‑94 (MQ=60)
                                  ATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGG                                                             >  SRR4302542.2539132/1‑94 (MQ=60)
                                  ATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGG                                                             <  SRR4302542.1151636/94‑1 (MQ=60)
                                  ATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGG                                                             >  SRR4302542.249520/1‑94 (MQ=60)
                                  ATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGG                                                             >  SRR4302542.409479/1‑94 (MQ=60)
                                   TGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGG                                                            <  SRR4302542.2291094/94‑1 (MQ=60)
                                   TGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGG                                                            >  SRR4302542.6132171/1‑94 (MQ=60)
                                    GATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGT                                                           >  SRR4302542.7094813/1‑94 (MQ=60)
                                      TACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTAT                                                         <  SRR4302542.4168914/94‑1 (MQ=60)
                                      TACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTAT                                                         <  SRR4302542.232832/94‑1 (MQ=60)
                                       ACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATC                                                        >  SRR4302542.4254533/1‑94 (MQ=60)
                                        CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGGGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCC                                                       >  SRR4302542.6280694/1‑94 (MQ=60)
                                        CTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCCGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCC                                                       <  SRR4302542.3290959/94‑1 (MQ=60)
                                         TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCT                                                      <  SRR4302542.1738915/94‑1 (MQ=60)
                                         TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCT                                                      >  SRR4302542.5719536/1‑94 (MQ=60)
                                         TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCT                                                      >  SRR4302542.5145306/1‑94 (MQ=60)
                                          TTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTG                                                     <  SRR4302542.3398330/94‑1 (MQ=60)
                                              ATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCG                                                 <  SRR4302542.6827505/94‑1 (MQ=60)
                                              ATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCG                                                 >  SRR4302542.2847947/1‑94 (MQ=60)
                                              ATCATGCCTACGCTCTCCACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTCCGCTGATCGTGGGTATCCTGTGCG                                                 >  SRR4302542.3883501/1‑94 (MQ=60)
                                               TCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGT                                                <  SRR4302542.3007543/94‑1 (MQ=60)
                                                 ATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTAT                                              <  SRR4302542.1662613/94‑1 (MQ=60)
                                                 ATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTAT                                              >  SRR4302542.1354241/1‑94 (MQ=60)
                                                   GCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCG                                            <  SRR4302542.322474/94‑1 (MQ=60)
                                                     CTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGT                                          >  SRR4302542.6639609/1‑94 (MQ=60)
                                                     CTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGT                                          >  SRR4302542.2135463/1‑94 (MQ=60)
                                                     CTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGT                                          <  SRR4302542.160566/94‑1 (MQ=60)
                                                      TACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTA                                         <  SRR4302542.3597633/94‑1 (MQ=60)
                                                      TACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTA                                         <  SRR4302542.3871395/94‑1 (MQ=60)
                                                       ACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAA                                        >  SRR4302542.6954249/1‑94 (MQ=60)
                                                        CGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAAT                                       >  SRR4302542.6065337/1‑94 (MQ=60)
                                                         GCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATG                                      <  SRR4302542.3251123/94‑1 (MQ=60)
                                                         GCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATG                                      >  SRR4302542.6888054/1‑94 (MQ=60)
                                                         GCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATG                                      <  SRR4302542.468408/94‑1 (MQ=60)
                                                         GCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATG                                      >  SRR4302542.7045277/1‑94 (MQ=60)
                                                         GCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATG                                      <  SRR4302542.814693/94‑1 (MQ=60)
                                                          CTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGT                                     >  SRR4302542.3086575/1‑94 (MQ=60)
                                                           TGTCAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCAATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTT                                    >  SRR4302542.6282610/1‑94 (MQ=60)
                                                            GTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTC                                   >  SRR4302542.1869233/1‑94 (MQ=60)
                                                              CAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTT                                 <  SRR4302542.5658559/94‑1 (MQ=60)
                                                              CAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTT                                 <  SRR4302542.5572938/94‑1 (MQ=60)
                                                              CAACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTT                                 <  SRR4302542.7178021/94‑1 (MQ=60)
                                                               AACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTT                                <  SRR4302542.1123280/94‑1 (MQ=60)
                                                                ACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTA                               >  SRR4302542.2491721/1‑94 (MQ=60)
                                                                ACGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTA                               <  SRR4302542.6734874/94‑1 (MQ=60)
                                                                 CGTATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAG                              <  SRR4302542.460726/94‑1 (MQ=60)
                                                                  GTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGC                             >  SRR4302542.1969533/1‑94 (MQ=60)
                                                                    ATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTA                           >  SRR4302542.5542860/1‑94 (MQ=60)
                                                                    ATCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTA                           >  SRR4302542.7393379/1‑94 (MQ=60)
                                                                     TCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAA                          <  SRR4302542.7159599/94‑1 (MQ=60)
                                                                     TCTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAA                          >  SRR4302542.4148235/1‑94 (MQ=60)
                                                                      CTCATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAAT                         <  SRR4302542.2888404/94‑1 (MQ=60)
                                                                        CATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATAT                       <  SRR4302542.7203685/94‑1 (MQ=60)
                                                                        CATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATAT                       <  SRR4302542.6318019/94‑1 (MQ=60)
                                                                        CATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATAT                       >  SRR4302542.400220/1‑94 (MQ=60)
                                                                         ATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATC                      <  SRR4302542.5931646/94‑1 (MQ=60)
                                                                         ATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATC                      >  SRR4302542.1638092/1‑94 (MQ=60)
                                                                         ATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATC                      <  SRR4302542.3682148/94‑1 (MQ=60)
                                                                         ATCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATC                      >  SRR4302542.4921425/1‑94 (MQ=60)
                                                                          TCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCA                     <  SRR4302542.2922779/94‑1 (MQ=60)
                                                                          TCCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCA                     <  SRR4302542.456523/94‑1 (MQ=60)
                                                                           CCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAG                    <  SRR4302542.4470678/94‑1 (MQ=60)
                                                                           CCCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAG                    <  SRR4302542.6841914/94‑1 (MQ=60)
                                                                            CCGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGT                   >  SRR4302542.6059827/1‑94 (MQ=60)
                                                                             CGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTT                  <  SRR4302542.6104155/94‑1 (MQ=60)
                                                                             CGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTT                  <  SRR4302542.4061840/94‑1 (MQ=60)
                                                                             CGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTT                  <  SRR4302542.1676302/94‑1 (MQ=60)
                                                                             CGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTT                  <  SRR4302542.3749223/94‑1 (MQ=60)
                                                                             CGGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTT                  <  SRR4302542.7282426/94‑1 (MQ=60)
                                                                              GGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTG                 >  SRR4302542.7392273/1‑94 (MQ=60)
                                                                              GGCGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTG                 <  SRR4302542.3828177/94‑1 (MQ=60)
                                                                                CGCTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTG               >  SRR4302542.7493729/1‑94 (MQ=60)
                                                                                  CTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGA             >  SRR4302542.701152/1‑94 (MQ=60)
                                                                                  CTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGA             <  SRR4302542.3808913/94‑1 (MQ=60)
                                                                                  CTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGA             >  SRR4302542.922596/1‑94 (MQ=60)
                                                                                  CTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGA             >  SRR4302542.6943525/1‑94 (MQ=60)
                                                                                  CTTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGA             >  SRR4302542.4729874/1‑94 (MQ=60)
                                                                                   TTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGAT            <  SRR4302542.6625170/94‑1 (MQ=60)
                                                                                   TTAAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGAT            <  SRR4302542.2375868/94‑1 (MQ=60)
                                                                                     AAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGG          <  SRR4302542.1120362/94‑1 (MQ=60)
                                                                                     AAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGG          <  SRR4302542.655289/94‑1 (MQ=60)
                                                                                     AAACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGG          <  SRR4302542.4245361/94‑1 (MQ=60)
                                                                                      AACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGT         <  SRR4302542.993038/94‑1 (MQ=60)
                                                                                       ACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTT        <  SRR4302542.2252986/94‑1 (MQ=60)
                                                                                       ACCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTT        <  SRR4302542.1495465/94‑1 (MQ=60)
                                                                                        CCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTC       >  SRR4302542.5308527/1‑94 (MQ=60)
                                                                                        CCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTC       >  SRR4302542.2132515/1‑94 (MQ=60)
                                                                                        CCCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTC       >  SRR4302542.1732353/1‑94 (MQ=60)
                                                                                         CCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCA      >  SRR4302542.36000/1‑94 (MQ=60)
                                                                                         CCTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCA      <  SRR4302542.1213302/94‑1 (MQ=60)
                                                                                          CTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAA     >  SRR4302542.2189359/1‑94 (MQ=60)
                                                                                          CTGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAA     >  SRR4302542.1412744/1‑94 (MQ=60)
                                                                                           TGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAA    >  SRR4302542.6618138/1‑94 (MQ=60)
                                                                                           TGACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAA    >  SRR4302542.4006429/1‑94 (MQ=60)
                                                                                            GACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAA   >  SRR4302542.4981767/1‑94 (MQ=60)
                                                                                            GACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAA   >  SRR4302542.3962841/1‑94 (MQ=60)
                                                                                            GACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAA   >  SRR4302542.310349/1‑94 (MQ=60)
                                                                                            GACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAA   <  SRR4302542.7478890/94‑1 (MQ=60)
                                                                                             ACGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAAT  <  SRR4302542.736896/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATGATTTACGCGATCAGCGGCCCGCTCATTGCCATGATACTTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAAT  >  NC_000913/2984936‑2985122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑