Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,996,557 T→C K112K (AAA→AAG)  b2863 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,996,5570TC89.4% 703.3 / 62.6 265K112K (AAA→AAG) b2863hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/10);  new base C (121/115);  total (140/125)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 266 total aligned reads displayed.

CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTG  >  NC_000913/2996468‑2996649
                                                                                         |                                                                                            
cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTtc                                                                                           >  2:6016117/1‑93 (MQ=255)
cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           >  1:6124647/1‑93 (MQ=255)
cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           <  1:3281920/93‑1 (MQ=255)
cTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtc                                                                                           >  2:5378843/1‑93 (MQ=255)
 tATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtct                                                                                          >  2:6916189/1‑93 (MQ=255)
 tATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtct                                                                                          >  1:6634886/1‑93 (MQ=255)
  aTCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtctc                                                                                         <  1:2875808/93‑1 (MQ=255)
  aTCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTtctc                                                                                         <  2:6462125/93‑1 (MQ=255)
    cccTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCg                                                                                       >  1:5111921/1‑93 (MQ=255)
     ccTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGt                                                                                      >  2:6600385/1‑93 (MQ=255)
      cTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTc                                                                                     <  2:837600/93‑1 (MQ=255)
      cTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTc                                                                                     >  2:1771601/1‑93 (MQ=255)
        cAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCaa                                                                                   <  2:6868913/93‑1 (MQ=255)
          aCCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACg                                                                                 <  2:4232525/93‑1 (MQ=255)
               aCAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAc                                                                            >  1:579048/1‑93 (MQ=255)
               aCAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAc                                                                            >  2:1334939/1‑93 (MQ=255)
                  gCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACttt                                                                         <  1:3996819/93‑1 (MQ=255)
                    atcatAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCg                                                                       >  1:2997183/1‑93 (MQ=255)
                     tcatAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgt                                                                      <  1:2716298/93‑1 (MQ=255)
                      catAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtg                                                                     >  1:6723027/1‑93 (MQ=255)
                       atAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtgt                                                                    <  2:7079510/93‑1 (MQ=255)
                        tAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCgtgtg                                                                   >  1:6904493/1‑93 (MQ=255)
                          gTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGtt                                                                 <  1:7325426/93‑1 (MQ=255)
                          gTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGtt                                                                 >  1:5505564/1‑93 (MQ=255)
                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                >  2:4904753/1‑93 (MQ=255)
                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                <  1:7287163/93‑1 (MQ=255)
                           tGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGttt                                                                <  1:2364863/93‑1 (MQ=255)
                             ggTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTc                                                              >  2:3251392/1‑93 (MQ=255)
                             ggTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTc                                                              <  2:1496852/93‑1 (MQ=255)
                              gTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAAAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCt                                                             <  2:1581071/93‑1 (MQ=255)
                               tttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTg                                                            <  1:1241640/93‑1 (MQ=255)
                               tttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTg                                                            >  1:3122502/1‑93 (MQ=255)
                                ttCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGt                                                           <  2:1925954/93‑1 (MQ=255)
                                   tGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTaa                                                        <  1:4958106/93‑1 (MQ=255)
                                    gCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAAt                                                       >  1:6286427/1‑93 (MQ=255)
                                     ccGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATg                                                      <  2:3473574/93‑1 (MQ=255)
                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    <  1:2037455/93‑1 (MQ=255)
                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGGTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    >  1:7374577/1‑93 (MQ=255)
                                       gCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGtt                                                    >  2:6082198/1‑93 (MQ=255)
                                        cTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTa                                                   <  2:5285457/93‑1 (MQ=255)
                                         tGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAg                                                  >  1:4522569/1‑93 (MQ=255)
                                          gTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGc                                                 <  2:2276316/93‑1 (MQ=255)
                                           ttttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCa                                                >  2:6303271/1‑93 (MQ=255)
                                           ttttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCa                                                >  2:7418306/1‑93 (MQ=255)
                                             ttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAt                                              <  2:5028417/93‑1 (MQ=255)
                                             ttGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAt                                              >  1:4248851/1‑93 (MQ=255)
                                               gTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATcc                                            <  2:1145956/93‑1 (MQ=255)
                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  1:4859684/1‑93 (MQ=255)
                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  2:5143717/1‑93 (MQ=255)
                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           <  2:2589160/93‑1 (MQ=255)
                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           <  2:4022268/93‑1 (MQ=255)
                                                tCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCt                                           >  2:4364149/1‑93 (MQ=255)
                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  2:2677421/93‑1 (MQ=255)
                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  1:4665856/93‑1 (MQ=255)
                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          >  2:449249/1‑93 (MQ=255)
                                                 cATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCtt                                          <  2:1135582/93‑1 (MQ=255)
                                                  aTCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTg                                         >  2:5812768/1‑93 (MQ=255)
                                                    cTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGaa                                       <  1:5720259/93‑1 (MQ=255)
                                                    cTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGaa                                       <  2:3934439/93‑1 (MQ=255)
                                                       cGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAAttt                                    <  1:7170356/93‑1 (MQ=255)
                                                         aaaGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCg                                  >  2:4991363/1‑93 (MQ=255)
                                                           aGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTc                                >  2:3645965/1‑93 (MQ=255)
                                                            gTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCt                               <  2:5871122/93‑1 (MQ=255)
                                                             tAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCtt                              >  1:3919030/1‑93 (MQ=255)
                                                              aGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCttt                             <  2:2747529/93‑1 (MQ=255)
                                                               gAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTg                            <  2:5476227/93‑1 (MQ=255)
                                                               gAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTg                            <  2:2510981/93‑1 (MQ=255)
                                                                aaGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTgg                           <  1:3740365/93‑1 (MQ=255)
                                                                 aGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGt                          <  2:6766412/93‑1 (MQ=255)
                                                                    aaGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGtata                       <  2:3017446/93‑1 (MQ=255)
                                                                    aaGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGtata                       >  2:2550956/1‑93 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  1:4453313/1‑93 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      <  2:5636699/93‑1 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      <  2:1946785/93‑1 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:3175360/1‑93 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:4324314/1‑93 (MQ=255)
                                                                     aGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAg                      >  2:2968060/1‑93 (MQ=255)
                                                                      gAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     >  1:103214/1‑93 (MQ=255)
                                                                      gAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTATTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     <  1:977826/93‑1 (MQ=255)
                                                                      gAATAAACGTTCCCCAGGGCTTCCCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGt                     >  1:2141430/1‑93 (MQ=255)
                                                                         tAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  <  1:4758532/93‑1 (MQ=255)
                                                                         tAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  >  1:336272/1‑93 (MQ=255)
                                                                         tAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGc                  >  1:5069272/1‑93 (MQ=255)
                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  1:6067180/93‑1 (MQ=255)
                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  2:1383873/93‑1 (MQ=255)
                                                                           aaCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctc                <  2:5451550/93‑1 (MQ=255)
                                                                            aCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctct               >  1:5919197/1‑93 (MQ=255)
                                                                            aCGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGctct               >  2:1400900/1‑93 (MQ=255)
                                                                               ttCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTa            <  2:4528954/93‑1 (MQ=255)
                                                                                tCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAt           >  2:7488500/1‑93 (MQ=255)
                                                                                  cccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAttt         >  2:4011446/1‑93 (MQ=255)
                                                                                  cccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTAttt         >  1:5532700/1‑93 (MQ=255)
                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        >  2:6173605/1‑93 (MQ=255)
                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        <  1:1771601/93‑1 (MQ=255)
                                                                                   ccAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTc        >  1:6497417/1‑93 (MQ=255)
                                                                                    cAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCa       >  2:4730446/1‑93 (MQ=255)
                                                                                     aGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAt      <  1:3514367/93‑1 (MQ=255)
                                                                                      gggTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAtt     <  1:7417378/93‑1 (MQ=255)
                                                                                      gggCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAtt     <  1:5166409/93‑1 (MQ=255)
                                                                                       ggTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCAttt    <  2:5211375/93‑1 (MQ=255)
                                                                                         |                                                                                            
CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTG  >  NC_000913/2996468‑2996649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  >  NC_000913/2996465‑2996650
                                                                                            |                                                                                             
AAACTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCT                                                                                              >  SRR4302542.3321587/1‑94 (MQ=60)
  ACTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTC                                                                                            >  SRR4302542.6183910/1‑94 (MQ=60)
  ACTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTC                                                                                            >  SRR4302542.3248181/1‑94 (MQ=60)
   CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCT                                                                                           <  SRR4302542.3312881/94‑1 (MQ=60)
   CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCT                                                                                           >  SRR4302542.4556031/1‑94 (MQ=60)
   CTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCT                                                                                           >  SRR4302542.6700294/1‑94 (MQ=60)
     ATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCC                                                                                         <  SRR4302542.2903420/94‑1 (MQ=60)
      TCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCG                                                                                        >  SRR4302542.5161569/1‑94 (MQ=60)
      TCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCG                                                                                        >  SRR4302542.4025581/1‑94 (MQ=60)
       CCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGT                                                                                       <  SRR4302542.3857083/94‑1 (MQ=60)
            AACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACG                                                                                  <  SRR4302542.6740254/94‑1 (MQ=60)
            AACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACG                                                                                  <  SRR4302542.2962607/94‑1 (MQ=60)
              CCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTT                                                                                >  SRR4302542.5785410/1‑94 (MQ=60)
                 GACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAC                                                                             >  SRR4302542.584059/1‑94 (MQ=60)
                 GACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAAC                                                                             <  SRR4302542.2937756/94‑1 (MQ=60)
                   CAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTT                                                                           <  SRR4302542.1691513/94‑1 (MQ=60)
                    AGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTT                                                                          >  SRR4302542.5810353/1‑94 (MQ=60)
                    AGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTT                                                                          >  SRR4302542.4969747/1‑94 (MQ=60)
                     GCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTC                                                                         <  SRR4302542.4036245/94‑1 (MQ=60)
                      CATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCG                                                                        >  SRR4302542.3025861/1‑94 (MQ=60)
                      CATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCG                                                                        <  SRR4302542.707578/94‑1 (MQ=60)
                      CATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACCTTCG                                                                        <  SRR4302542.7036988/94‑1 (MQ=60)
                       ATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGT                                                                       <  SRR4302542.5723763/94‑1 (MQ=60)
                        TCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTG                                                                      <  SRR4302542.2742493/94‑1 (MQ=60)
                        TCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTG                                                                      >  SRR4302542.6789226/1‑94 (MQ=60)
                        TCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTG                                                                      >  SRR4302542.6191945/1‑94 (MQ=60)
                         CATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGT                                                                     >  SRR4302542.6671969/1‑94 (MQ=60)
                         CATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGT                                                                     >  SRR4302542.1089149/1‑94 (MQ=60)
                          ATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTG                                                                    >  SRR4302542.6972280/1‑94 (MQ=60)
                            AGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTT                                                                  >  SRR4302542.5559074/1‑94 (MQ=60)
                             GTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTT                                                                 <  SRR4302542.7397183/94‑1 (MQ=60)
                              TGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTT                                                                <  SRR4302542.7358603/94‑1 (MQ=60)
                              TGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTT                                                                <  SRR4302542.2385311/94‑1 (MQ=60)
                               GGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTC                                                               <  SRR4302542.2103414/94‑1 (MQ=60)
                                 GTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTG                                                             >  SRR4302542.3152203/1‑94 (MQ=60)
                                  TTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGT                                                            <  SRR4302542.1252312/94‑1 (MQ=60)
                                   TTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTT                                                           <  SRR4302542.2458348/94‑1 (MQ=60)
                                     CTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAA                                                         >  SRR4302542.5576717/1‑94 (MQ=60)
                                     CTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAA                                                         <  SRR4302542.7418101/94‑1 (MQ=60)
                                     CTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAA                                                         >  SRR4302542.2255951/1‑94 (MQ=60)
                                     CTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAA                                                         <  SRR4302542.7418102/94‑1 (MQ=60)
                                      TGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAAT                                                        <  SRR4302542.786758/94‑1 (MQ=60)
                                      TGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAAT                                                        <  SRR4302542.5006271/94‑1 (MQ=60)
                                      TGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAAT                                                        >  SRR4302542.6348374/1‑94 (MQ=60)
                                       GCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATG                                                       <  SRR4302542.3129428/94‑1 (MQ=60)
                                        CCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGT                                                      <  SRR4302542.5759240/94‑1 (MQ=60)
                                         CGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGGTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTT                                                     >  SRR4302542.7446790/1‑94 (MQ=60)
                                         CGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTT                                                     <  SRR4302542.5213544/94‑1 (MQ=60)
                                         CGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTT                                                     >  SRR4302542.2289357/1‑94 (MQ=60)
                                          GCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTA                                                    <  SRR4302542.2055071/94‑1 (MQ=60)
                                          GCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTA                                                    >  SRR4302542.7387342/1‑94 (MQ=60)
                                           CTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAG                                                   >  SRR4302542.4566875/1‑94 (MQ=60)
                                           CTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAG                                                   >  SRR4302542.1835077/1‑94 (MQ=60)
                                           CTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAG                                                   >  SRR4302542.3529913/1‑94 (MQ=60)
                                            TGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGC                                                  <  SRR4302542.6517222/94‑1 (MQ=60)
                                             GTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCA                                                 <  SRR4302542.467248/94‑1 (MQ=60)
                                              TTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAA                                                >  SRR4302542.2056716/1‑94 (MQ=60)
                                               TTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAT                                               >  SRR4302542.4290710/1‑94 (MQ=60)
                                               TTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAAT                                               <  SRR4302542.6908395/94‑1 (MQ=60)
                                                 TGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCC                                             <  SRR4302542.2886962/94‑1 (MQ=60)
                                                 TGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCC                                             <  SRR4302542.5018349/94‑1 (MQ=60)
                                                  GTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCT                                            >  SRR4302542.4907014/1‑94 (MQ=60)
                                                   TCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTT                                           <  SRR4302542.4299366/94‑1 (MQ=60)
                                                    CATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTG                                          <  SRR4302542.4711467/94‑1 (MQ=60)
                                                    CATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTG                                          <  SRR4302542.4273821/94‑1 (MQ=60)
                                                     ATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGA                                         >  SRR4302542.3654219/1‑94 (MQ=60)
                                                     ATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGA                                         >  SRR4302542.473758/1‑94 (MQ=60)
                                                     ATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGA                                         >  SRR4302542.5208915/1‑94 (MQ=60)
                                                     ATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGA                                         >  SRR4302542.4398893/1‑94 (MQ=60)
                                                      TCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAA                                        <  SRR4302542.3339756/94‑1 (MQ=60)
                                                      TCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAA                                        >  SRR4302542.3389771/1‑94 (MQ=60)
                                                       CTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAAT                                       <  SRR4302542.6112225/94‑1 (MQ=60)
                                                       CTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAAT                                       <  SRR4302542.5775783/94‑1 (MQ=60)
                                                         TCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTT                                     <  SRR4302542.6136453/94‑1 (MQ=60)
                                                          CGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTC                                    <  SRR4302542.7240686/94‑1 (MQ=60)
                                                           GAAAGTAGAAGCAAGAATAAACGTT‑CCCAGGGCTTCTCCGTCCACGTTAACTTTCGTGTGGTTTTTTTTTATTTTAGCAATCCTTTAATTTCGT                                  >  SRR4302542.5131223/1‑94 (MQ=48)
                                                            AAAGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGT                                  <  SRR4302542.4684297/94‑1 (MQ=60)
                                                              AGTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCT                                <  SRR4302542.5864435/94‑1 (MQ=60)
                                                               GTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTT                               <  SRR4302542.7451679/94‑1 (MQ=60)
                                                               GTAGAAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTT                               >  SRR4302542.3957699/1‑94 (MQ=60)
                                                                   AAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGT                           >  SRR4302542.4750746/1‑94 (MQ=60)
                                                                   AAGCAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGT                           <  SRR4302542.3777384/94‑1 (MQ=60)
                                                                      CAAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATA                        >  SRR4302542.6858088/1‑94 (MQ=60)
                                                                       AAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAG                       >  SRR4302542.5381591/1‑94 (MQ=60)
                                                                       AAGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAG                       >  SRR4302542.4496999/1‑94 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      >  SRR4302542.6487820/1‑94 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      <  SRR4302542.736130/94‑1 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      >  SRR4302542.104071/1‑94 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      >  SRR4302542.6000271/1‑94 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      >  SRR4302542.3298648/1‑94 (MQ=60)
                                                                        AGAATAAACGTTCCCCAGGGCTTCCCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGT                      >  SRR4302542.2159903/1‑94 (MQ=60)
                                                                         GAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTG                     >  SRR4302542.5051837/1‑94 (MQ=60)
                                                                         GAATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTATTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTG                     <  SRR4302542.986256/94‑1 (MQ=60)
                                                                          AATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGG                    <  SRR4302542.3572039/94‑1 (MQ=60)
                                                                           ATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGC                   >  SRR4302542.339193/1‑94 (MQ=60)
                                                                           ATAAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGC                   >  SRR4302542.5118533/1‑94 (MQ=60)
                                                                            TAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCT                  <  SRR4302542.4804935/94‑1 (MQ=60)
                                                                             AAACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTC                 >  SRR4302542.3913013/1‑94 (MQ=60)
                                                                              AACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCT                <  SRR4302542.6125939/94‑1 (MQ=60)
                                                                              AACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCT                >  SRR4302542.2803071/1‑94 (MQ=60)
                                                                              AACGTTCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCT                >  SRR4302542.5976557/1‑94 (MQ=60)
                                                                                   TCCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATT           <  SRR4302542.4159622/94‑1 (MQ=60)
                                                                                    CCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTT          >  SRR4302542.6599678/1‑94 (MQ=60)
                                                                                    CCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTT          >  SRR4302542.5586475/1‑94 (MQ=60)
                                                                                    CCCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTT          <  SRR4302542.6154140/94‑1 (MQ=60)
                                                                                     CCCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTC         >  SRR4302542.6561449/1‑94 (MQ=60)
                                                                                      CCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCA        >  SRR4302542.4987696/1‑94 (MQ=60)
                                                                                      CCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCA        <  SRR4302542.5325944/94‑1 (MQ=60)
                                                                                      CCAGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCA        <  SRR4302542.1786960/94‑1 (MQ=60)
                                                                                        AGGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATT      <  SRR4302542.3547222/94‑1 (MQ=60)
                                                                                         GGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     <  SRR4302542.7489959/94‑1 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     <  SRR4302542.1744607/94‑1 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     <  SRR4302542.835453/94‑1 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     >  SRR4302542.705710/1‑94 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     <  SRR4302542.7247754/94‑1 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     <  SRR4302542.5216621/94‑1 (MQ=60)
                                                                                         GGGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTT     >  SRR4302542.5570499/1‑94 (MQ=60)
                                                                                          GGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTT    <  SRR4302542.3029812/94‑1 (MQ=60)
                                                                                          GGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTT    <  SRR4302542.6828552/94‑1 (MQ=60)
                                                                                          GGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTT    <  SRR4302542.5403949/94‑1 (MQ=60)
                                                                                          GGCTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTT    >  SRR4302542.1255994/1‑94 (MQ=60)
                                                                                            TTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  <  SRR4302542.487526/94‑1 (MQ=60)
                                                                                            CTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  >  SRR4302542.3874279/1‑94 (MQ=60)
                                                                                            CTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  >  SRR4302542.3527320/1‑94 (MQ=60)
                                                                                            CTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  <  SRR4302542.5125286/94‑1 (MQ=60)
                                                                                            |                                                                                             
AAACTATCCCTCAACCAGACAGCATCATAGTGGGTTTCTGCCGCTGTTTTGTCATCTTCGAAAGTAGAAGCAAGAATAAACGTTCCCCAGGGTTTCTCCGTCAACGTTAACTTTCGTGTGTTTTCTGTTAATGTTAGCAATCCTTGAATTTCGTCTTTGGTATAGTGGCTCTTTATTTCATTTTGT  >  NC_000913/2996465‑2996650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑