Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,074,951	C→T	G80S (GGC→AGC)	b2931 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,074,951	0	C	T	87.9%	915.2 / 67.6	306	G80S (GGC→AGC)	b2931	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (15/22); new base T (119/150); total (134/172)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 314 total aligned reads displayed.

ACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCT  >  NC_000913/3074860‑3075040

accaccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTgccg                                                                                          >  2:1641244/1‑93 (MQ=255)

  caccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTgctgcc                                                                                        <  2:6593063/93‑1 (MQ=255)

   accCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCa                                                                                       >  1:5946634/1‑93 (MQ=255)

    nncgcgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAg                                                                                      <  2:2125430/91‑1 (MQ=255)

    cccGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAg                                                                                      <  2:6231480/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:6762199/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:3772028/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  1:2237817/93‑1 (MQ=255)

        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGttt                                                                                  <  2:1428399/93‑1 (MQ=255)

            ggTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACg                                                                              >  2:5844952/1‑93 (MQ=255)

             gTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGc                                                                             >  1:1996312/1‑93 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          >  2:7130863/1‑93 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          <  1:5494199/93‑1 (MQ=255)

                gTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCa                                                                          <  2:5587553/93‑1 (MQ=255)

                   tGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGc                                                                       >  1:3314506/1‑93 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGa                                                                     <  2:90627/93‑1 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGa                                                                     <  2:1284118/93‑1 (MQ=255)

                     ccgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                                     <  1:6395441/93‑1 (MQ=255)

                      cgccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAt                                                                    >  2:2755307/1‑93 (MQ=255)

                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGc                                                                  >  1:5324452/1‑93 (MQ=255)

                          gTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTc                                                                >  1:1890943/1‑93 (MQ=255)

                          gTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTc                                                                >  1:2956549/1‑93 (MQ=255)

                           ttAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCa                                                               >  2:4646718/1‑93 (MQ=255)

                           ttAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCa                                                               >  1:875270/1‑93 (MQ=255)

                            tAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCAt                                                              >  1:3187634/1‑93 (MQ=255)

                              aTGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATaa                                                            >  2:4164959/1‑93 (MQ=255)

                               tGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATaaa                                                           >  2:4420547/1‑93 (MQ=255)

                                ggCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAAc                                                          >  2:2868339/1‑93 (MQ=255)

                                ggCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAAc                                                          <  2:4041193/93‑1 (MQ=255)

                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACat                                                        <  2:189882/93‑1 (MQ=255)

                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACat                                                        <  2:3533796/93‑1 (MQ=255)

                                   gTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACata                                                       >  2:1720751/1‑93 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  1:950749/93‑1 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  1:5797089/93‑1 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      >  2:4567133/1‑93 (MQ=255)

                                    tAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACatat                                                      <  2:5653191/93‑1 (MQ=255)

                                      aTCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTg                                                    <  2:4873919/93‑1 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCGCACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgt                                                 <  2:3302193/93‑1 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgt                                                 >  2:768825/1‑93 (MQ=255)

                                         gATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATANTGCgt                                                 >  1:6491833/1‑93 (MQ=255)

                                          aTAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtg                                                >  2:3147547/1‑93 (MQ=255)

                                          aTAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtg                                                <  2:1077066/93‑1 (MQ=255)

                                           tAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtgg                                               <  2:6982817/93‑1 (MQ=255)

                                           tAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgtgg                                               <  1:413720/93‑1 (MQ=255)

                                               gcCAATGCCCATCGGACCTGCACGCGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGtgtg                                           <  1:1389032/93‑1 (MQ=255)

                                                 cAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGAt                                         <  1:100033/93‑1 (MQ=255)

                                                  aaTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATa                                        <  1:5085171/93‑1 (MQ=255)

                                                  aaTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATa                                        >  1:4338852/1‑93 (MQ=255)

                                                   aTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATaa                                       <  1:2530368/93‑1 (MQ=255)

                                                   aTGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATaa                                       >  2:6343996/1‑93 (MQ=255)

                                                    tGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                                      >  1:3621986/1‑93 (MQ=255)

                                                    tGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                                      >  1:4993983/1‑93 (MQ=255)

                                                      cccATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTg                                    >  1:4721737/1‑93 (MQ=255)

                                                      cccATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTg                                    <  2:1203741/93‑1 (MQ=255)

                                                       ccATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTgg                                   <  1:3030810/93‑1 (MQ=255)

                                                        cATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGc                                  >  1:2653585/1‑93 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 >  2:3988925/1‑93 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  2:3839165/93‑1 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  1:819129/93‑1 (MQ=255)

                                                         aTCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                                 <  2:5914078/93‑1 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                >  1:3702499/1‑93 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                <  1:2435008/93‑1 (MQ=255)

                                                          tCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAt                                <  1:7000012/93‑1 (MQ=255)

                                                              aCCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATa                            <  1:603342/93‑1 (MQ=255)

                                                               ccTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATaa                           <  1:7114421/93‑1 (MQ=255)

                                                               ccTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATaa                           >  1:7400190/1‑93 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          <  2:820820/93‑1 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          >  1:1276984/1‑93 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          <  2:2370293/93‑1 (MQ=255)

                                                                cTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAg                          >  1:4383742/1‑93 (MQ=255)

                                                                 tGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                         >  1:4908654/1‑93 (MQ=255)

                                                                    acacGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCAcc                      <  1:5881084/93‑1 (MQ=255)

                                                                      acGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaa                    <  1:6814845/93‑1 (MQ=255)

                                                                       cGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat                   <  1:4605612/93‑1 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                >  2:5260152/1‑93 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                <  2:5377373/93‑1 (MQ=255)

                                                                          cagcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaataat                <  1:2880470/93‑1 (MQ=255)

                                                                           agcagTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg               <  1:7295953/93‑1 (MQ=255)

                                                                           agcagTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg               >  2:6379933/1‑93 (MQ=255)

                                                                              agTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcag            >  2:4762074/1‑93 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTTATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           >  1:6226764/1‑93 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           <  2:2512083/93‑1 (MQ=255)

                                                                               gTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagc           >  2:4948766/1‑93 (MQ=255)

                                                                                tGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagca          >  2:5505701/1‑93 (MQ=255)

                                                                                tGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgcagca          >  2:360672/1‑93 (MQ=255)

                                                                                 gCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcac         >  2:7394640/1‑93 (MQ=255)

                                                                                  cAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcaca        <  2:6727135/93‑1 (MQ=255)

                                                                                  cAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcaca        <  2:4321759/93‑1 (MQ=255)

                                                                                   aaTATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACAt       >  1:1610235/1‑93 (MQ=255)

                                                                                    atatTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATa      <  1:1673694/93‑1 (MQ=255)

                                                                                    atatTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATa      <  2:3704421/93‑1 (MQ=255)

                                                                                     tatTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAg     <  1:2920094/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:3893573/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:3462125/93‑1 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    >  2:2269154/1‑93 (MQ=255)

                                                                                      atTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAgg    <  1:745745/93‑1 (MQ=255)

                                                                                       ttGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGc   >  2:5168553/1‑93 (MQ=255)

                                                                                        tgctgcCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  <  2:6101249/93‑1 (MQ=255)

                                                                                        tgctgcCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  >  2:7263706/1‑93 (MQ=255)

                                                                                        tGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGct  <  2:3273205/93‑1 (MQ=255)

ACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCT  >  NC_000913/3074860‑3075040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTCTG  >  NC_000913/3074858‑3075043

CCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCT                                                                                              <  SRR4302542.6199649/94‑1 (MQ=60)

CAACCGCCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCTCACGCCAGCAGTGCAATATTGCT                                                                                              <  SRR4302542.6103830/94‑1 (MQ=60)

 CACCACCCGCGACGGTTGTATGCCGCTGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTG                                                                                             <  SRR4302542.3187401/94‑1 (MQ=60)

 CACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTG                                                                                             >  SRR4302542.3263739/1‑94 (MQ=60)

 CACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTG                                                                                             <  SRR4302542.5910061/94‑1 (MQ=60)

 CACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTG                                                                                             >  SRR4302542.5837383/1‑94 (MQ=60)

    CACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCA                                                                                          >  SRR4302542.6004231/1‑94 (MQ=60)

     CCCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAG                                                                                         <  SRR4302542.6398077/94‑1 (MQ=60)

     ACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAG                                                                                         >  SRR4302542.5814233/1‑94 (MQ=60)

     ACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAG                                                                                         <  SRR4302542.3388444/94‑1 (MQ=60)

        CGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTT                                                                                      >  SRR4302542.3781496/1‑94 (MQ=60)

        CGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTT                                                                                      <  SRR4302542.6042128/94‑1 (MQ=60)

        CGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTT                                                                                      <  SRR4302542.913056/94‑1 (MQ=60)

         GCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTT                                                                                     >  SRR4302542.6548714/1‑94 (MQ=60)

         GCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTT                                                                                     <  SRR4302542.7531289/94‑1 (MQ=60)

         GCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTT                                                                                     <  SRR4302542.6134739/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    <  SRR4302542.3809335/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    <  SRR4302542.2257062/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    <  SRR4302542.5885974/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    <  SRR4302542.1063367/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    >  SRR4302542.1973745/1‑94 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTG                                                                                    <  SRR4302542.6828714/94‑1 (MQ=60)

          CGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTG                                                                                    <  SRR4302542.6866004/94‑1 (MQ=60)

           GACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGA                                                                                   >  SRR4302542.3075424/1‑94 (MQ=60)

             CGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACG                                                                                 >  SRR4302542.4333196/1‑94 (MQ=60)

             CGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACG                                                                                 <  SRR4302542.4381576/94‑1 (MQ=60)

              GGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGC                                                                                >  SRR4302542.2013533/1‑94 (MQ=60)

              GGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGC                                                                                >  SRR4302542.4089463/1‑94 (MQ=60)

              GGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGC                                                                                >  SRR4302542.4094751/1‑94 (MQ=60)

                TTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCC                                                                              <  SRR4302542.508902/94‑1 (MQ=60)

                 TGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCA                                                                             >  SRR4302542.4414963/1‑94 (MQ=60)

                  GTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCAT                                                                            <  SRR4302542.6114174/94‑1 (MQ=60)

                  GTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCAT                                                                            <  SRR4302542.5547564/94‑1 (MQ=60)

                  CTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCAT                                                                            <  SRR4302542.5506643/94‑1 (MQ=60)

                   TATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATG                                                                           >  SRR4302542.2001981/1‑94 (MQ=60)

                   TATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATG                                                                           >  SRR4302542.367398/1‑94 (MQ=60)

                   TATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATG                                                                           <  SRR4302542.437126/94‑1 (MQ=60)

                    ATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGC                                                                          <  SRR4302542.543251/94‑1 (MQ=60)

                    ATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGC                                                                          >  SRR4302542.3345746/1‑94 (MQ=60)

                    ATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGC                                                                          <  SRR4302542.1639180/94‑1 (MQ=60)

                     TGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCG                                                                         <  SRR4302542.1774843/94‑1 (MQ=60)

                     TGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCG                                                                         <  SRR4302542.4797631/94‑1 (MQ=60)

                       CGGCCGTTAATGGCGTAATCGATAGCACCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.7605363/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTCATCGATAGCGCCAATGCCCATCGGACCTGCACAAGCCAGCAGTGCAATATTGCCGCAAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.2950889/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.2474208/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.1638523/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.6748743/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.5719722/94‑1 (MQ=60)

                       CCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGAT                                                                       <  SRR4302542.6458447/94‑1 (MQ=60)

                        CGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATG                                                                      >  SRR4302542.663081/1‑94 (MQ=60)

                        CGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATG                                                                      <  SRR4302542.3207573/94‑1 (MQ=60)

                         GCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGC                                                                     >  SRR4302542.998924/1‑94 (MQ=60)

                         GCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGC                                                                     >  SRR4302542.5376182/1‑94 (MQ=60)

                          CCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCT                                                                    <  SRR4302542.5915637/94‑1 (MQ=60)

                           CGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTC                                                                   >  SRR4302542.1907306/1‑94 (MQ=60)

                           CGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTC                                                                   <  SRR4302542.3177814/94‑1 (MQ=60)

                           CGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTC                                                                   >  SRR4302542.2984857/1‑94 (MQ=60)

                            GTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCA                                                                  >  SRR4302542.882822/1‑94 (MQ=60)

                            GTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCA                                                                  >  SRR4302542.5261799/1‑94 (MQ=60)

                            GTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCA                                                                  <  SRR4302542.701694/94‑1 (MQ=60)

                             TTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCAT                                                                 >  SRR4302542.3217829/1‑94 (MQ=60)

                              TAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATA                                                                >  SRR4302542.4006007/1‑94 (MQ=60)

                                    CGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATA                                                          <  SRR4302542.6026186/94‑1 (MQ=60)

                                     GTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATAT                                                         >  SRR4302542.3038457/1‑94 (MQ=60)

                                     GTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATAT                                                         >  SRR4302542.3870048/1‑94 (MQ=60)

                                     GTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATAT                                                         <  SRR4302542.27367/94‑1 (MQ=60)

                                     GTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATAT                                                         <  SRR4302542.893702/94‑1 (MQ=60)

                                      TAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATT                                                        >  SRR4302542.2475861/1‑94 (MQ=60)

                                      TAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATT                                                        <  SRR4302542.5853358/94‑1 (MQ=60)

                                      TAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATT                                                        >  SRR4302542.6449122/1‑94 (MQ=60)

                                      TAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATT                                                        <  SRR4302542.958916/94‑1 (MQ=60)

                                       AATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTG                                                       >  SRR4302542.3458094/1‑94 (MQ=60)

                                        ATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGC                                                      <  SRR4302542.1369397/94‑1 (MQ=60)

                                          CGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGT                                                    >  SRR4302542.7295146/1‑94 (MQ=60)

                                          CGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGT                                                    >  SRR4302542.7451252/1‑94 (MQ=60)

                                          CGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGT                                                    <  SRR4302542.1143244/94‑1 (MQ=60)

                                          CGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATANTGCGT                                                    >  SRR4302542.6555797/1‑94 (MQ=60)

                                            ATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGG                                                  >  SRR4302542.296748/1‑94 (MQ=60)

                                            ATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGG                                                  <  SRR4302542.5090514/94‑1 (MQ=60)

                                             TAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGT                                                 <  SRR4302542.417277/94‑1 (MQ=60)

                                              AGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTG                                                <  SRR4302542.2913890/94‑1 (MQ=60)

                                              AGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTG                                                <  SRR4302542.7092246/94‑1 (MQ=60)

                                                CGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTG                                              >  SRR4302542.2183162/1‑94 (MQ=60)

                                                 GCCAATGCCCATCGGACCTGCACGCGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGA                                             <  SRR4302542.1401026/94‑1 (MQ=60)

                                                 GCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGA                                             >  SRR4302542.5976324/1‑94 (MQ=60)

                                                  CCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGAT                                            >  SRR4302542.245156/1‑94 (MQ=60)

                                                   CAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATA                                           <  SRR4302542.100876/94‑1 (MQ=60)

                                                   CAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATA                                           <  SRR4302542.718936/94‑1 (MQ=60)

                                                   CAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATA                                           >  SRR4302542.4381484/1‑94 (MQ=60)

                                                   CAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATA                                           <  SRR4302542.4511342/94‑1 (MQ=60)

                                                   CAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATA                                           >  SRR4302542.501341/1‑94 (MQ=60)

                                                    AATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAA                                          <  SRR4302542.5134591/94‑1 (MQ=60)

                                                     ATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAT                                         >  SRR4302542.5042496/1‑94 (MQ=60)

                                                     ATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAT                                         >  SRR4302542.2427645/1‑94 (MQ=60)

                                                     ATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAT                                         >  SRR4302542.3655727/1‑94 (MQ=60)

                                                     ATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAT                                         <  SRR4302542.2554912/94‑1 (MQ=60)

                                                      TGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATT                                        <  SRR4302542.2934644/94‑1 (MQ=60)

                                                      TGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATT                                        >  SRR4302542.1710775/1‑94 (MQ=60)

                                                       GCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTG                                       <  SRR4302542.3231825/94‑1 (MQ=60)

                                                       GCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTG                                       >  SRR4302542.4767760/1‑94 (MQ=60)

                                                       GCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTG                                       <  SRR4302542.7529320/94‑1 (MQ=60)

                                                         CCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGC                                     <  SRR4302542.6864689/94‑1 (MQ=60)

                                                         CCATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGC                                     >  SRR4302542.2679214/1‑94 (MQ=60)

                                                         CCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGC                                     <  SRR4302542.3059730/94‑1 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   >  SRR4302542.2557422/1‑94 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   <  SRR4302542.826209/94‑1 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   >  SRR4302542.828247/1‑94 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   >  SRR4302542.4896419/1‑94 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   >  SRR4302542.3739138/1‑94 (MQ=60)

                                                           ATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCAT                                   <  SRR4302542.2989931/94‑1 (MQ=60)

                                                            TCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATG                                  <  SRR4302542.2458700/94‑1 (MQ=60)

                                                            TCGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATG                                  <  SRR4302542.7068777/94‑1 (MQ=60)

                                                             CGGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGA                                 >  SRR4302542.2606281/1‑94 (MQ=60)

                                                             CGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGA                                 >  SRR4302542.3862852/1‑94 (MQ=60)

                                                              GGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGAT                                >  SRR4302542.1556408/1‑94 (MQ=60)

                                                              GGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGAT                                <  SRR4302542.7284685/94‑1 (MQ=60)

                                                              GGACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGAT                                >  SRR4302542.4992685/1‑94 (MQ=60)

                                                               GACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATA                               <  SRR4302542.70831/94‑1 (MQ=60)

                                                                ACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAA                              <  SRR4302542.608540/94‑1 (MQ=60)

                                                                ACCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAA                              >  SRR4302542.7472610/1‑94 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             <  SRR4302542.7184262/94‑1 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             <  SRR4302542.3663271/94‑1 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             >  SRR4302542.1287948/1‑94 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             >  SRR4302542.3558062/1‑94 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             >  SRR4302542.5087956/1‑94 (MQ=60)

                                                                 CCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAG                             >  SRR4302542.4426760/1‑94 (MQ=60)

                                                                  CTGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGC                            >  SRR4302542.4956398/1‑94 (MQ=60)

                                                                   TGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCA                           <  SRR4302542.875081/94‑1 (MQ=60)

                                                                   TGCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCA                           >  SRR4302542.4761500/1‑94 (MQ=60)

                                                                    GCACACGCCGGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCAC                          >  SRR4302542.6196953/1‑94 (MQ=60)

                                                                    GCACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCAC                          <  SRR4302542.242016/94‑1 (MQ=60)

                                                                      ACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCA                        <  SRR4302542.3336654/94‑1 (MQ=60)

                                                                      ACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCA                        <  SRR4302542.5938097/94‑1 (MQ=60)

                                                                      ACACGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCA                        <  SRR4302542.6962237/94‑1 (MQ=60)

                                                                        ACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAAT                      <  SRR4302542.6881843/94‑1 (MQ=60)

                                                                         CGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGTTGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATA                     <  SRR4302542.7447219/94‑1 (MQ=60)

                                                                         CGCCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATA                     <  SRR4302542.4650623/94‑1 (MQ=60)

                                                                          GCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAA                    >  SRR4302542.2066621/1‑94 (MQ=60)

                                                                           CCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAAT                   <  SRR4302542.5199221/94‑1 (MQ=60)

                                                                           CCAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAAT                   <  SRR4302542.3534901/94‑1 (MQ=60)

                                                                            CAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATG                  >  SRR4302542.4763061/1‑94 (MQ=60)

                                                                            CAGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATG                  <  SRR4302542.2908138/94‑1 (MQ=60)

                                                                             AGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGC                 <  SRR4302542.1518918/94‑1 (MQ=60)

                                                                             AGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGC                 <  SRR4302542.5977785/94‑1 (MQ=60)

                                                                             AGCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGC                 <  SRR4302542.7367452/94‑1 (MQ=60)

                                                                              GCAGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCA                <  SRR4302542.2745953/94‑1 (MQ=60)

                                                                                AGTGCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTTATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGC              >  SRR4302542.6288081/1‑94 (MQ=60)

                                                                                   GCAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACA           <  SRR4302542.1297140/94‑1 (MQ=60)

                                                                                    CAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACAT          <  SRR4302542.3789973/94‑1 (MQ=60)

                                                                                    CAATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACAT          >  SRR4302542.1624139/1‑94 (MQ=60)

                                                                                      ATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAG        <  SRR4302542.1688158/94‑1 (MQ=60)

                                                                                      ATATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAG        >  SRR4302542.6465467/1‑94 (MQ=60)

                                                                                       TATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGG       >  SRR4302542.7489626/1‑94 (MQ=60)

                                                                                       TATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGG       <  SRR4302542.2948083/94‑1 (MQ=60)

                                                                                        ATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGC      >  SRR4302542.4549731/1‑94 (MQ=60)

                                                                                        ATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGC      <  SRR4302542.752204/94‑1 (MQ=60)

                                                                                        ATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGC      <  SRR4302542.3932011/94‑1 (MQ=60)

                                                                                        ATTGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGC      <  SRR4302542.3494546/94‑1 (MQ=60)

                                                                                          TGCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTC    >  SRR4302542.5357299/1‑94 (MQ=60)

                                                                                           GCTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTCT   <  SRR4302542.2777202/94‑1 (MQ=60)

                                                                                            CTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTCTG  <  SRR4302542.2417915/94‑1 (MQ=60)

                                                                                            CTGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTCTG  >  SRR4302542.1804703/1‑94 (MQ=60)

CCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCACATAGGCTCTG  >  NC_000913/3074858‑3075043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`