Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,151,557 C→T intergenic (+119/‑21) metC → / → yghB cystathionine beta‑lyase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,151,5570CT87.6% 783.0 / 61.8 267intergenic (+119/‑21)metC/yghBcystathionine beta‑lyase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/15);  new base T (112/122);  total (130/137)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 271 total aligned reads displayed.

GACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT  >  NC_000913/3151465‑3151649
                                                                                            |                                                                                            
gACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAAc                                                                                              <  1:1015736/93‑1 (MQ=255)
gACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAAc                                                                                              >  1:6725028/1‑93 (MQ=255)
gACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAAc                                                                                              <  2:6081293/93‑1 (MQ=255)
  cATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGc                                                                                            >  2:5005668/1‑93 (MQ=255)
  cATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGc                                                                                            <  2:3974065/93‑1 (MQ=255)
   aTTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCt                                                                                           >  1:2274033/1‑93 (MQ=255)
   aTTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCt                                                                                           <  2:3939431/93‑1 (MQ=255)
    ttttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTg                                                                                          >  1:462078/1‑93 (MQ=255)
    ttttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTg                                                                                          <  1:3925803/93‑1 (MQ=255)
     tttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCTGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGt                                                                                         <  1:1456289/93‑1 (MQ=255)
     tttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGt                                                                                         <  2:6581782/93‑1 (MQ=255)
      ttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTGAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGtt                                                                                        >  2:1839110/1‑93 (MQ=255)
      ttATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGtt                                                                                        >  1:6636907/1‑93 (MQ=255)
        aTTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTcc                                                                                      >  2:7018299/1‑93 (MQ=255)
         ttGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCca                                                                                     >  2:3284435/1‑93 (MQ=255)
         ttGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCca                                                                                     <  1:4341909/93‑1 (MQ=255)
           gtgAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCcaca                                                                                   <  2:4711357/93‑1 (MQ=255)
           gtgAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCcaca                                                                                   >  2:6485911/1‑93 (MQ=255)
            tgAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAg                                                                                  <  1:2196454/93‑1 (MQ=255)
            tgAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAg                                                                                  >  2:6201876/1‑93 (MQ=255)
             gAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAgg                                                                                 >  2:4169827/1‑93 (MQ=255)
             gAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAgg                                                                                 >  2:1409992/1‑93 (MQ=255)
              aaaaGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGa                                                                                <  2:2248699/93‑1 (MQ=255)
                aaGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGaaa                                                                              <  1:6317390/93‑1 (MQ=255)
                aaGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGaaa                                                                              <  2:1131581/93‑1 (MQ=255)
                aaGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGaaa                                                                              <  2:267054/93‑1 (MQ=255)
                 aGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAg                                                                             >  2:709929/1‑93 (MQ=255)
                  gTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGGGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGt                                                                            >  2:1567930/1‑93 (MQ=255)
                  gTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGt                                                                            <  1:561709/93‑1 (MQ=255)
                   tCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTc                                                                           >  2:356390/1‑93 (MQ=255)
                    cTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTcc                                                                          >  1:4405393/1‑93 (MQ=255)
                    cTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTcc                                                                          >  2:7170512/1‑93 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCa                                                                         >  1:4575043/1‑93 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCa                                                                         <  2:3005658/93‑1 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCa                                                                         <  2:7080701/93‑1 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCa                                                                         >  1:7184458/1‑93 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCa                                                                         <  1:3105505/93‑1 (MQ=255)
                     ttAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCa                                                                         >  1:5389463/1‑93 (MQ=255)
                      tAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCAt                                                                        <  1:1050611/93‑1 (MQ=255)
                      tAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCAt                                                                        <  1:708829/93‑1 (MQ=255)
                       aaaTTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATg                                                                       >  2:3169855/1‑93 (MQ=255)
                       aaaTTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATg                                                                       <  2:1006352/93‑1 (MQ=255)
                        aaTTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATgg                                                                      >  1:5753772/1‑93 (MQ=255)
                         aTTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGc                                                                     <  2:2039667/93‑1 (MQ=255)
                         aTTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGc                                                                     >  1:3703453/1‑93 (MQ=255)
                          ttgttgCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCt                                                                    >  2:2606893/1‑93 (MQ=255)
                          ttgttgCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGGACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCt                                                                    >  1:6863590/1‑93 (MQ=255)
                                cGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTAtt                                                              <  2:2988138/93‑1 (MQ=255)
                                   ccGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATCCaa                                                           <  1:7502638/93‑1 (MQ=255)
                                     gggATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa                                                         >  1:2754889/1‑93 (MQ=255)
                                      ggATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat                                                        <  2:1194147/93‑1 (MQ=255)
                                      ggATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat                                                        >  2:7528701/1‑93 (MQ=255)
                                      ggACCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat                                                        >  2:3343527/1‑93 (MQ=255)
                                       gATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGata                                                       <  1:4779203/93‑1 (MQ=255)
                                       gATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGata                                                       >  1:5839713/1‑93 (MQ=255)
                                         tCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc                                                     >  1:5201150/1‑93 (MQ=255)
                                         tCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc                                                     <  1:4161824/93‑1 (MQ=255)
                                          cAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca                                                    <  1:3741149/93‑1 (MQ=255)
                                           aaGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat                                                   >  2:6767062/1‑93 (MQ=255)
                                             ggCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCg                                                 <  2:820378/93‑1 (MQ=255)
                                              gCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc                                                <  1:5782269/93‑1 (MQ=255)
                                                  cccGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg                                            >  1:25141/1‑93 (MQ=255)
                                                     ggACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctc                                         >  1:6017091/1‑93 (MQ=255)
                                                     ggACGATTCAGGAGTACAATAGGCAGAGAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctc                                         >  2:6416314/1‑93 (MQ=255)
                                                        cGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTgg                                      >  2:6135423/1‑93 (MQ=255)
                                                           ttCAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa                                   >  1:7318312/1‑93 (MQ=255)
                                                            tCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAac                                  >  2:2782468/1‑93 (MQ=255)
                                                             cAGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                 <  2:5756021/93‑1 (MQ=255)
                                                              aGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac                                <  1:1917136/93‑1 (MQ=255)
                                                              aGGAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac                                >  1:1657660/1‑93 (MQ=255)
                                                               ggAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACg                               <  1:2919643/93‑1 (MQ=255)
                                                               ggAGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACg                               >  1:948326/1‑93 (MQ=255)
                                                                 aGTACAATAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc                             <  2:5149308/93‑1 (MQ=255)
                                                                   tACAATAGGCAGATAAAGGCTTAAATGCTGTTCCATAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACtt                           <  2:4975385/93‑1 (MQ=255)
                                                                      aaTAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                        <  1:3213362/93‑1 (MQ=255)
                                                                      aaTAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                        >  2:2913040/1‑93 (MQ=255)
                                                                      aaTAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                        <  2:5583247/93‑1 (MQ=255)
                                                                      aaTAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                        >  2:6510768/1‑93 (MQ=255)
                                                                          ggCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgcg                    >  2:1606421/1‑93 (MQ=255)
                                                                           gCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                   <  2:7263047/93‑1 (MQ=255)
                                                                           gCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                   <  2:3416524/93‑1 (MQ=255)
                                                                           gCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                   <  1:3474094/93‑1 (MQ=255)
                                                                            cAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                  <  1:1446157/93‑1 (MQ=255)
                                                                              gATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg                <  1:4960113/93‑1 (MQ=255)
                                                                              gATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg                <  2:6442258/93‑1 (MQ=255)
                                                                               aTAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGc               <  2:4812426/93‑1 (MQ=255)
                                                                               aTAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGc               <  2:1656699/93‑1 (MQ=255)
                                                                                 aaaGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCgg             >  2:910201/1‑93 (MQ=255)
                                                                                 aaaGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCgg             <  2:1266042/93‑1 (MQ=255)
                                                                                 aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCgg             >  2:4768426/1‑93 (MQ=255)
                                                                                  aaGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa            <  1:1715270/93‑1 (MQ=255)
                                                                                   aGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAt           <  1:2401462/93‑1 (MQ=255)
                                                                                   aGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAt           >  1:5088989/1‑93 (MQ=255)
                                                                                     gCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATcc         <  1:4517639/93‑1 (MQ=255)
                                                                                       ttAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTc       <  1:127329/93‑1 (MQ=255)
                                                                                       ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTc       <  2:1317012/93‑1 (MQ=255)
                                                                                        tAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGCTCCTCa      >  1:3254092/1‑93 (MQ=255)
                                                                                        tAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa      >  1:3138990/1‑93 (MQ=255)
                                                                                        tAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa      >  1:2556503/1‑93 (MQ=255)
                                                                                         aaaTGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCat     <  1:5455222/93‑1 (MQ=255)
                                                                                            |                                                                                            
GACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT  >  NC_000913/3151465‑3151649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  >  NC_000913/3151464‑3151650
                                                                                                       |                                                                                             
AGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAAC                                                                                               >  SRR4302542.6791243/1‑94 (MQ=60)
 GACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATG                                                                                              <  SRR4302542.4791394/94‑1 (MQ=60)
 GACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACG                                                                                              <  SRR4302542.1024485/94‑1 (MQ=60)
  ACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGC                                                                                             <  SRR4302542.2999366/94‑1 (MQ=60)
  ACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGC                                                                                             >  SRR4302542.6822748/1‑94 (MQ=60)
   CATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCT                                                                                            <  SRR4302542.3606612/94‑1 (MQ=60)
   CATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCT                                                                                            >  SRR4302542.3740639/1‑94 (MQ=60)
   CATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCT                                                                                            >  SRR4302542.2293578/1‑94 (MQ=60)
    ATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTG                                                                                           >  SRR4302542.466079/1‑94 (MQ=60)
    ATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTG                                                                                           <  SRR4302542.1675026/94‑1 (MQ=60)
     TTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGT                                                                                          >  SRR4302542.3206606/1‑94 (MQ=60)
     TTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGT                                                                                          <  SRR4302542.3964540/94‑1 (MQ=60)
     TTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGT                                                                                          >  SRR4302542.6167511/1‑94 (MQ=60)
      TTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCTGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTT                                                                                         <  SRR4302542.1468858/94‑1 (MQ=60)
      TTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTT                                                                                         >  SRR4302542.6933673/1‑94 (MQ=60)
      TTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTT                                                                                         >  SRR4302542.6702326/1‑94 (MQ=60)
      TTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTT                                                                                         >  SRR4302542.2280103/1‑94 (MQ=60)
       TTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTC                                                                                        >  SRR4302542.4666347/1‑94 (MQ=60)
        TATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCC                                                                                       <  SRR4302542.7259834/94‑1 (MQ=60)
        TATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCC                                                                                       >  SRR4302542.2401911/1‑94 (MQ=60)
         ATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCA                                                                                      >  SRR4302542.5671829/1‑94 (MQ=60)
          TTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCAC                                                                                     <  SRR4302542.4384568/94‑1 (MQ=60)
           TGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACA                                                                                    >  SRR4302542.4569308/1‑94 (MQ=60)
             TGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGG                                                                                  <  SRR4302542.2215369/94‑1 (MQ=60)
             TGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGG                                                                                  <  SRR4302542.4284494/94‑1 (MQ=60)
              GAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGA                                                                                 >  SRR4302542.5081001/1‑94 (MQ=60)
               AAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAA                                                                                >  SRR4302542.4671183/1‑94 (MQ=60)
                AAAGTCTTAAATTGTTGCGGCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAA                                                                               >  SRR4302542.5336452/1‑94 (MQ=60)
                 AAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAG                                                                              <  SRR4302542.1981875/94‑1 (MQ=60)
                 AAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAG                                                                              <  SRR4302542.6379657/94‑1 (MQ=60)
                 AAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAG                                                                              <  SRR4302542.3341177/94‑1 (MQ=60)
                  AGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGT                                                                             <  SRR4302542.1127467/94‑1 (MQ=60)
                  AGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGT                                                                             >  SRR4302542.3793288/1‑94 (MQ=60)
                  AGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGT                                                                             <  SRR4302542.4350978/94‑1 (MQ=60)
                   GTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTC                                                                            >  SRR4302542.6185122/1‑94 (MQ=60)
                   GTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTC                                                                            >  SRR4302542.6421188/1‑94 (MQ=60)
                   GTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTC                                                                            <  SRR4302542.566550/94‑1 (MQ=60)
                    TCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCC                                                                           >  SRR4302542.4448658/1‑94 (MQ=60)
                     CTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCA                                                                          >  SRR4302542.4619806/1‑94 (MQ=60)
                     CTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCA                                                                          >  SRR4302542.7254910/1‑94 (MQ=60)
                     CTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCA                                                                          <  SRR4302542.970679/94‑1 (MQ=60)
                     CTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCA                                                                          >  SRR4302542.5441806/1‑94 (MQ=60)
                      TTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCAT                                                                         <  SRR4302542.3135024/94‑1 (MQ=60)
                      TTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCAT                                                                         <  SRR4302542.1782238/94‑1 (MQ=60)
                      TTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCAT                                                                         <  SRR4302542.2170898/94‑1 (MQ=60)
                       TAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATG                                                                        <  SRR4302542.714948/94‑1 (MQ=60)
                       TAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATG                                                                        >  SRR4302542.4167384/1‑94 (MQ=60)
                       TAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATG                                                                        <  SRR4302542.1059649/94‑1 (MQ=60)
                       TAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATG                                                                        <  SRR4302542.5151785/94‑1 (MQ=60)
                        AAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGG                                                                       >  SRR4302542.3056988/1‑94 (MQ=60)
                        AAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGG                                                                       >  SRR4302542.5809626/1‑94 (MQ=60)
                         AATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGC                                                                      >  SRR4302542.1496655/1‑94 (MQ=60)
                         AATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGC                                                                      >  SRR4302542.3740102/1‑94 (MQ=60)
                          ATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCT                                                                     <  SRR4302542.6724413/94‑1 (MQ=60)
                          ATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGGACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCT                                                                     >  SRR4302542.6931025/1‑94 (MQ=60)
                            TGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGT                                                                   <  SRR4302542.5811363/94‑1 (MQ=60)
                             nnnnnntncnGGANNANGGCGTCCNGGNCGNTTCANGAGTACAAT‑‑‑‑‑‑‑‑‑‑ANGCAGATNNANNCNTNAATGCTGTTCCACAGGAAAGTCCATGGCTGTT                                                                  <  SRR4302542.6344922/84‑1 (MQ=60)
                              TTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTA                                                                 <  SRR4302542.2990212/94‑1 (MQ=60)
                               TGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTAT                                                                <  SRR4302542.2248141/94‑1 (MQ=60)
                               TGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTAT                                                                >  SRR4302542.5456843/1‑94 (MQ=60)
                                GCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATT                                                               <  SRR4302542.6553325/94‑1 (MQ=60)
                                 CGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTC                                                              >  SRR4302542.7451224/1‑94 (MQ=60)
                                  GTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCA                                                             >  SRR4302542.7080364/1‑94 (MQ=60)
                                    CCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAG                                                           <  SRR4302542.7429352/94‑1 (MQ=60)
                                    CCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAG                                                           >  SRR4302542.1370295/1‑94 (MQ=60)
                                    CCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAG                                                           <  SRR4302542.343978/94‑1 (MQ=60)
                                    CCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATCCAAG                                                           <  SRR4302542.7576437/94‑1 (MQ=60)
                                    CCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAG                                                           <  SRR4302542.4478228/94‑1 (MQ=60)
                                     CGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGA                                                          >  SRR4302542.2781438/1‑94 (MQ=60)
                                      GGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGAT                                                         >  SRR4302542.442123/1‑94 (MQ=60)
                                      GGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGAT                                                         >  SRR4302542.1633201/1‑94 (MQ=60)
                                       GGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATA                                                        >  SRR4302542.5896367/1‑94 (MQ=60)
                                        GATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATAT                                                       <  SRR4302542.4825791/94‑1 (MQ=60)
                                        GATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATAT                                                       >  SRR4302542.5610499/1‑94 (MQ=60)
                                        GATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCAGTTATTCAAGATAT                                                       <  SRR4302542.3252107/94‑1 (MQ=60)
                                         ATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATC                                                      >  SRR4302542.5251689/1‑94 (MQ=60)
                                         ATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATC                                                      >  SRR4302542.3816806/1‑94 (MQ=60)
                                          TCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCA                                                     <  SRR4302542.4202815/94‑1 (MQ=60)
                                           CAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCAT                                                    <  SRR4302542.3778172/94‑1 (MQ=60)
                                           CAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCAT                                                    <  SRR4302542.6067791/94‑1 (MQ=60)
                                           CAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCAT                                                    <  SRR4302542.1067755/94‑1 (MQ=60)
                                            AAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATC                                                   >  SRR4302542.5730233/1‑94 (MQ=60)
                                            AAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATC                                                   <  SRR4302542.4121990/94‑1 (MQ=60)
                                             AGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCG                                                  >  SRR4302542.5491904/1‑94 (MQ=60)
                                             AGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCG                                                  <  SRR4302542.1716329/94‑1 (MQ=60)
                                               GCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGA                                                          <  SRR4302542.7188909/94‑1 (MQ=60)
                                               GCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCT                                                <  SRR4302542.5838415/94‑1 (MQ=60)
                                                 GTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGC                                              >  SRR4302542.6831193/1‑94 (MQ=60)
                                                 GTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGC                                              >  SRR4302542.863810/1‑94 (MQ=60)
                                                  TCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCG                                             >  SRR4302542.25361/1‑94 (MQ=60)
                                                     CGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTC                                          >  SRR4302542.6075363/1‑94 (MQ=60)
                                                      GGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCT                                         <  SRR4302542.5075557/94‑1 (MQ=60)
                                                      GGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCT                                         >  SRR4302542.3137444/1‑94 (MQ=60)
                                                       GACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTG                                        <  SRR4302542.2569749/94‑1 (MQ=60)
                                                        ACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGG                                       <  SRR4302542.1463301/94‑1 (MQ=60)
                                                          GATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCA                                     >  SRR4302542.5860825/1‑94 (MQ=60)
                                                           ATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAA                                    >  SRR4302542.7389989/1‑94 (MQ=60)
                                                              CAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACAC                                 >  SRR4302542.1671986/1‑94 (MQ=60)
                                                               AGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACG                                >  SRR4302542.3233800/1‑94 (MQ=60)
                                                               AGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACG                                >  SRR4302542.956479/1‑94 (MQ=60)
                                                               AGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACG                                <  SRR4302542.1933702/94‑1 (MQ=60)
                                                                GGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGA                               <  SRR4302542.2947630/94‑1 (MQ=60)
                                                                 GAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAC                              <  SRR4302542.2570391/94‑1 (MQ=60)
                                                                    TACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTT                           <  SRR4302542.5828485/94‑1 (MQ=60)
                                                                    TACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTT                           <  SRR4302542.7601553/94‑1 (MQ=60)
                                                                     ACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTG                          >  SRR4302542.461245/1‑94 (MQ=60)
                                                                     ACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTG                          <  SRR4302542.3522048/94‑1 (MQ=60)
                                                                      CAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGC                         >  SRR4302542.4645233/1‑94 (MQ=60)
                                                                       AAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCC                        <  SRR4302542.2498169/94‑1 (MQ=60)
                                                                       AAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCC                        <  SRR4302542.3243774/94‑1 (MQ=60)
                                                                        AT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCG                       >  SRR4302542.7202538/1‑94 (MQ=60)
                                                                                      GCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCT                   <  SRR4302542.3506615/94‑1 (MQ=60)
                                                                                       CAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTG                  <  SRR4302542.1458634/94‑1 (MQ=60)
                                                                                       CAGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTG                  <  SRR4302542.1041809/94‑1 (MQ=60)
                                                                                        AGATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGG                 >  SRR4302542.263491/1‑94 (MQ=60)
                                                                                         GATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGC                <  SRR4302542.2589672/94‑1 (MQ=60)
                                                                                         GATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGC                <  SRR4302542.5008295/94‑1 (MQ=60)
                                                                                         GATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGC                <  SRR4302542.4484368/94‑1 (MQ=60)
                                                                                          ATAAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCG               <  SRR4302542.5317789/94‑1 (MQ=60)
                                                                                            AAAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGA             >  SRR4302542.3155904/1‑94 (MQ=60)
                                                                                             AAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAT            <  SRR4302542.1730170/94‑1 (MQ=60)
                                                                                             AAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAT            <  SRR4302542.499540/94‑1 (MQ=60)
                                                                                             AAGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAT            >  SRR4302542.5138452/1‑94 (MQ=60)
                                                                                              AGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATC           <  SRR4302542.2424842/94‑1 (MQ=60)
                                                                                              AGGCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATC           <  SRR4302542.2424841/94‑1 (MQ=60)
                                                                                                GCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCT         >  SRR4302542.1391530/1‑94 (MQ=60)
                                                                                                GCTTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCT         <  SRR4302542.4561902/94‑1 (MQ=60)
                                                                                                GCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCT         >  SRR4302542.2871741/1‑94 (MQ=60)
                                                                                                  TTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGCTCCTCA       >  SRR4302542.3284826/1‑94 (MQ=60)
                                                                                                  TTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCA       <  SRR4302542.128368/94‑1 (MQ=60)
                                                                                                  TTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCA       >  SRR4302542.3168827/1‑94 (MQ=60)
                                                                                                  TTAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCA       >  SRR4302542.2581289/1‑94 (MQ=60)
                                                                                                   TAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACGCGACTTTGCCGCGCTGGCGGATCCTCAT      <  SRR4302542.6015577/94‑1 (MQ=60)
                                                                                                   TAAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCAT      >  SRR4302542.493495/1‑94 (MQ=60)
                                                                                                    AAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATA     >  SRR4302542.6841699/1‑94 (MQ=60)
                                                                                                    AAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATA     <  SRR4302542.7313020/94‑1 (MQ=60)
                                                                                                    AAATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATA     <  SRR4302542.5508216/94‑1 (MQ=60)
                                                                                                     AATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAT    <  SRR4302542.5196326/94‑1 (MQ=60)
                                                                                                     AATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAT    <  SRR4302542.5301715/94‑1 (MQ=60)
                                                                                                     AATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAT    <  SRR4302542.3814943/94‑1 (MQ=60)
                                                                                                      ATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT   >  SRR4302542.7269560/1‑94 (MQ=60)
                                                                                                      ATGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT   <  SRR4302542.3761737/94‑1 (MQ=60)
                                                                                                       TGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  <  SRR4302542.1886298/94‑1 (MQ=60)
                                                                                                       TGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  >  SRR4302542.2691790/1‑94 (MQ=60)
                                                                                                       TGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  >  SRR4302542.5445120/1‑94 (MQ=60)
                                                                                                       TGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  <  SRR4302542.5091146/94‑1 (MQ=60)
                                                                                                       CGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  >  SRR4302542.2572267/1‑94 (MQ=60)
                                                                                                       |                                                                                             
AGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAAT‑‑‑‑‑‑‑‑‑‑AGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTG  >  NC_000913/3151464‑3151650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑