Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,186,925 A→G T504A (ACT→GCT)  yqiG → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,186,9250AG83.0% 648.7 / 129.4 294T504A (ACT→GCT) yqiGputative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (31/19);  new base G (139/105);  total (170/124)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-01

Only 100 of 311 total aligned reads displayed.

TATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTC  >  NC_000913/3186835‑3187015
                                                                                          |                                                                                          
tATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgct                                                                                          <  2:6358485/93‑1 (MQ=255)
tATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgct                                                                                          >  2:4144848/1‑93 (MQ=255)
  tCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgctta                                                                                        <  2:6844523/93‑1 (MQ=255)
   cAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgcttac                                                                                       <  2:4827880/93‑1 (MQ=255)
    aGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgcttact                                                                                      >  2:5648958/1‑93 (MQ=255)
    aGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgcttact                                                                                      >  1:3716401/1‑93 (MQ=255)
    aGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAAtgcttact                                                                                      >  1:3802150/1‑93 (MQ=255)
     gAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTg                                                                                     >  2:5265028/1‑93 (MQ=255)
     gAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTg                                                                                     >  1:4765721/1‑93 (MQ=255)
        tGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGa                                                                                  <  2:862272/93‑1 (MQ=255)
        tGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGa                                                                                  <  2:2810791/93‑1 (MQ=255)
            aaaGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGcc                                                                              <  2:2483202/93‑1 (MQ=255)
               gagagTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCcagc                                                                           <  1:16707/93‑1 (MQ=255)
               gagagTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCcagc                                                                           >  1:1000350/1‑93 (MQ=255)
               gagagTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCcagc                                                                           >  1:2893414/1‑93 (MQ=255)
                agagTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCcagca                                                                          >  2:5784318/1‑93 (MQ=255)
                agagTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCcagca                                                                          <  2:7297752/93‑1 (MQ=255)
                   gTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTa                                                                       >  1:3632247/1‑93 (MQ=255)
                    ttATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTaa                                                                      <  2:1848105/93‑1 (MQ=255)
                    ttATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTaa                                                                      >  2:4956537/1‑93 (MQ=255)
                     tataTTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAt                                                                     <  1:1429425/93‑1 (MQ=255)
                     tataTTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAt                                                                     >  1:2084085/1‑93 (MQ=255)
                     tataTTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAt                                                                     >  2:2639815/1‑93 (MQ=255)
                       tatTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAtgt                                                                   >  1:5563203/1‑93 (MQ=255)
                       tatTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAtgt                                                                   <  1:4438851/93‑1 (MQ=255)
                       tatTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAtgt                                                                   <  1:518012/93‑1 (MQ=255)
                       tatTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAtgt                                                                   <  2:4338645/93‑1 (MQ=255)
                        atTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAtgtg                                                                  >  2:6552858/1‑93 (MQ=255)
                         ttGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGa                                                                 >  2:3354576/1‑93 (MQ=255)
                          tGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGaa                                                                >  1:7050058/1‑93 (MQ=255)
                           gTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAat                                                               <  2:4919063/93‑1 (MQ=255)
                           gTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAat                                                               <  2:2729043/93‑1 (MQ=255)
                           gTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAat                                                               <  1:4102942/93‑1 (MQ=255)
                               cGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAattatt                                                           >  2:6785463/1‑93 (MQ=255)
                                gTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTc                                                          <  1:6966462/93‑1 (MQ=255)
                                gTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTc                                                          <  2:5060267/93‑1 (MQ=255)
                                 tttAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTCATGTGAATTATTCa                                                         <  2:7485580/93‑1 (MQ=255)
                                 tttAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCa                                                         <  2:1937280/93‑1 (MQ=255)
                                 tttAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCa                                                         >  2:3886401/1‑93 (MQ=255)
                                  ttAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCAt                                                        <  1:5458271/93‑1 (MQ=255)
                                    aaCCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTa                                                      >  1:2440498/1‑93 (MQ=255)
                                     aCCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTAt                                                     <  2:1761495/93‑1 (MQ=255)
                                      ccAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATc                                                    <  1:5311540/93‑1 (MQ=255)
                                      ccAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATc                                                    >  1:3020449/1‑93 (MQ=255)
                                       cAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCa                                                   <  1:4619060/93‑1 (MQ=255)
                                       cAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCa                                                   <  1:4177543/93‑1 (MQ=255)
                                       cAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCa                                                   <  2:2395724/93‑1 (MQ=255)
                                        aGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAc                                                  >  1:7162520/1‑93 (MQ=255)
                                        aGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAc                                                  >  2:6040899/1‑93 (MQ=255)
                                          tATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACtt                                                <  1:6498083/93‑1 (MQ=255)
                                          tATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACtt                                                >  1:7422783/1‑93 (MQ=255)
                                          tATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACtt                                                >  2:2139759/1‑93 (MQ=255)
                                           aTCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTa                                               >  2:1397551/1‑93 (MQ=255)
                                            tCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAg                                              >  2:1550834/1‑93 (MQ=255)
                                            tCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAg                                              >  1:6325187/1‑93 (MQ=255)
                                            tCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAg                                              <  1:1241146/93‑1 (MQ=255)
                                              ttGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGcc                                            >  2:552424/1‑93 (MQ=255)
                                              ttGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGcc                                            <  2:1240597/93‑1 (MQ=255)
                                              ttGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGcc                                            <  1:5680389/93‑1 (MQ=255)
                                               tGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCcg                                           >  1:57116/1‑93 (MQ=255)
                                               tGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCcg                                           >  2:6198318/1‑93 (MQ=255)
                                                gAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCcgc                                          >  2:5479930/1‑93 (MQ=255)
                                                 aaTCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCcgcg                                         >  2:2986563/1‑93 (MQ=255)
                                                  aTCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGa                                        <  2:253701/93‑1 (MQ=255)
                                                     aTTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAttt                                     >  1:223590/1‑93 (MQ=255)
                                                     aTTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAttt                                     >  2:356313/1‑93 (MQ=255)
                                                     aTTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAttt                                     >  2:3801371/1‑93 (MQ=255)
                                                      ttAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAtttt                                    <  1:6004283/93‑1 (MQ=255)
                                                      ttAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAtttt                                    >  2:5338192/1‑93 (MQ=255)
                                                       tAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTg                                   <  2:7172991/93‑1 (MQ=255)
                                                        aaGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGa                                  <  2:5147214/93‑1 (MQ=255)
                                                          ggTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGata                                <  1:4848633/93‑1 (MQ=255)
                                                          ggTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGata                                <  1:1839204/93‑1 (MQ=255)
                                                          ggTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGata                                >  2:5826455/1‑93 (MQ=255)
                                                           gTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGatat                               <  1:4258034/93‑1 (MQ=255)
                                                             tAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCg                             <  1:1086046/93‑1 (MQ=255)
                                                               aaTACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCggg                           >  2:230034/1‑93 (MQ=255)
                                                               aaTACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCggg                           >  2:4248272/1‑93 (MQ=255)
                                                               aaTACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCggg                           >  2:3446550/1‑93 (MQ=255)
                                                                 tacatacGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGcc                         <  1:3356202/93‑1 (MQ=255)
                                                                 tacatacGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGcc                         <  1:991195/93‑1 (MQ=255)
                                                                  acatacGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCa                        <  2:4543180/93‑1 (MQ=255)
                                                                     tacGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTa                     >  2:6930325/1‑93 (MQ=255)
                                                                         tAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTaaaaa                 <  1:6135624/93‑1 (MQ=255)
                                                                           aGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAc               <  2:1295542/93‑1 (MQ=255)
                                                                           aGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAc               <  1:2767213/93‑1 (MQ=255)
                                                                           aGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAc               >  1:1914017/1‑93 (MQ=255)
                                                                           aGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAc               >  2:3658215/1‑93 (MQ=255)
                                                                           aGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGAAATCGGGCCATTAAAAAAc               <  1:4914085/93‑1 (MQ=255)
                                                                            gTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACg              >  1:3424237/1‑93 (MQ=255)
                                                                            gTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACg              >  2:6669754/1‑93 (MQ=255)
                                                                              ttGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGtc            <  1:2347187/93‑1 (MQ=255)
                                                                               tGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGtct           <  2:4689042/93‑1 (MQ=255)
                                                                                ggCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGtctc          <  2:4022222/93‑1 (MQ=255)
                                                                                ggCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGtctc          <  1:722702/93‑1 (MQ=255)
                                                                                ggCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGtctc          >  1:138818/1‑93 (MQ=255)
                                                                                 gCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTcc         >  1:4484617/1‑93 (MQ=255)
                                                                                 gCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTcc         >  2:4508798/1‑93 (MQ=255)
                                                                                  cTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCa        <  1:3269510/93‑1 (MQ=255)
                                                                                    cGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACt      <  1:1988807/93‑1 (MQ=255)
                                                                                          |                                                                                          
TATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTC  >  NC_000913/3186835‑3187015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTAA  >  NC_000913/3186832‑3187018
                                                                                             |                                                                                             
AATTATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATG                                                                                               >  SRR4302542.5929933/1‑94 (MQ=60)
  TTATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCT                                                                                             >  SRR4302542.1939234/1‑94 (MQ=60)
  TTATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCT                                                                                             >  SRR4302542.3674947/1‑94 (MQ=60)
    ATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTA                                                                                           >  SRR4302542.181828/1‑94 (MQ=60)
     TCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTAC                                                                                          <  SRR4302542.6011457/94‑1 (MQ=60)
      CAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACT                                                                                         >  SRR4302542.3839785/1‑94 (MQ=60)
      CAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACT                                                                                         >  SRR4302542.3753163/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTG                                                                                        >  SRR4302542.4812179/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTG                                                                                        >  SRR4302542.3386452/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTG                                                                                        >  SRR4302542.1433292/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTG                                                                                        >  SRR4302542.2312055/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTG                                                                                        >  SRR4302542.4994829/1‑94 (MQ=60)
       AGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTG                                                                                        >  SRR4302542.1724994/1‑94 (MQ=60)
        GAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGG                                                                                       >  SRR4302542.1062076/1‑94 (MQ=60)
         AATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGG                                                                                      <  SRR4302542.3804260/94‑1 (MQ=60)
         AATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGG                                                                                      >  SRR4302542.6968589/1‑94 (MQ=60)
           TGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGAC                                                                                    <  SRR4302542.1434720/94‑1 (MQ=60)
            GAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACG                                                                                   >  SRR4302542.2058189/1‑94 (MQ=60)
            GAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACG                                                                                   >  SRR4302542.4753065/1‑94 (MQ=60)
            GAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACG                                                                                   <  SRR4302542.1116474/94‑1 (MQ=60)
               AAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCA                                                                                >  SRR4302542.5258644/1‑94 (MQ=60)
               AAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCA                                                                                >  SRR4302542.4433467/1‑94 (MQ=60)
               AAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCA                                                                                >  SRR4302542.186339/1‑94 (MQ=60)
               AAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCA                                                                                >  SRR4302542.3501550/1‑94 (MQ=60)
                 AGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGC                                                                              >  SRR4302542.7296065/1‑94 (MQ=60)
                 AGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGC                                                                              >  SRR4302542.2921189/1‑94 (MQ=60)
                 AGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGC                                                                              >  SRR4302542.1008957/1‑94 (MQ=60)
                  GAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCA                                                                             <  SRR4302542.16852/94‑1 (MQ=60)
                    GAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGT                                                                           >  SRR4302542.528020/1‑94 (MQ=60)
                     AGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTA                                                                          >  SRR4302542.3666093/1‑94 (MQ=60)
                       TTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAT                                                                        >  SRR4302542.2102081/1‑94 (MQ=60)
                       TTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAT                                                                        >  SRR4302542.3677142/1‑94 (MQ=60)
                       TTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAAT                                                                        >  SRR4302542.728108/1‑94 (MQ=60)
                       TTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAAT                                                                        <  SRR4302542.3098258/94‑1 (MQ=60)
                        TATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATG                                                                       <  SRR4302542.1441788/94‑1 (MQ=60)
                        TATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATG                                                                       >  SRR4302542.575374/1‑94 (MQ=60)
                        TATATTGTCACGTTTAACCAGTATCTTGAACCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATG                                                                       <  SRR4302542.1614045/94‑1 (MQ=60)
                         ATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGT                                                                      >  SRR4302542.5617259/1‑94 (MQ=60)
                          TATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTG                                                                     <  SRR4302542.4482415/94‑1 (MQ=60)
                          TATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTG                                                                     >  SRR4302542.3901144/1‑94 (MQ=60)
                          TATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTG                                                                     <  SRR4302542.522499/94‑1 (MQ=60)
                           ATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGA                                                                    >  SRR4302542.5748446/1‑94 (MQ=60)
                           ATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGA                                                                    >  SRR4302542.6030244/1‑94 (MQ=60)
                            TTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAA                                                                   >  SRR4302542.7119271/1‑94 (MQ=60)
                              GTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATT                                                                 <  SRR4302542.4143391/94‑1 (MQ=60)
                              GTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATT                                                                 >  SRR4302542.7014643/1‑94 (MQ=60)
                               TCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTA                                                                >  SRR4302542.4639078/1‑94 (MQ=60)
                                CACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTAT                                                               <  SRR4302542.4667017/94‑1 (MQ=60)
                                 ACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATT                                                              >  SRR4302542.4266722/1‑94 (MQ=60)
                                 ACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATT                                                              <  SRR4302542.2641224/94‑1 (MQ=60)
                                  CGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTC                                                             >  SRR4302542.778395/1‑94 (MQ=60)
                                  CGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTC                                                             >  SRR4302542.7390770/1‑94 (MQ=60)
                                   GTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCA                                                            >  SRR4302542.5537170/1‑94 (MQ=60)
                                   GTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCA                                                            <  SRR4302542.7034907/94‑1 (MQ=60)
                                    TTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCAT                                                           <  SRR4302542.4884457/94‑1 (MQ=60)
                                    TTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCAT                                                           >  SRR4302542.7229990/1‑94 (MQ=60)
                                     TTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATT                                                          <  SRR4302542.5511300/94‑1 (MQ=60)
                                      TAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTA                                                         >  SRR4302542.2051779/1‑94 (MQ=60)
                                      TAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTA                                                         >  SRR4302542.4857232/1‑94 (MQ=60)
                                      TAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTA                                                         >  SRR4302542.2464246/1‑94 (MQ=60)
                                      TAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTA                                                         >  SRR4302542.3428854/1‑94 (MQ=60)
                                       AACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTAT                                                        >  SRR4302542.341437/1‑94 (MQ=60)
                                        ACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATC                                                       >  SRR4302542.461548/1‑94 (MQ=60)
                                        ACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATC                                                       >  SRR4302542.3049296/1‑94 (MQ=60)
                                         CCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCA                                                      <  SRR4302542.5363142/94‑1 (MQ=60)
                                          CAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAC                                                     <  SRR4302542.4664186/94‑1 (MQ=60)
                                          CAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAC                                                     <  SRR4302542.4218737/94‑1 (MQ=60)
                                          CAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAC                                                     >  SRR4302542.2381968/1‑94 (MQ=60)
                                          CAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAC                                                     >  SRR4302542.7232778/1‑94 (MQ=60)
                                          CAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCAC                                                     >  SRR4302542.1373268/1‑94 (MQ=60)
                                           AGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACT                                                    >  SRR4302542.1897865/1‑94 (MQ=60)
                                            GTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTT                                                   >  SRR4302542.7495416/1‑94 (MQ=60)
                                            GTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTT                                                   <  SRR4302542.4525062/94‑1 (MQ=60)
                                             TATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTA                                                  <  SRR4302542.6562124/94‑1 (MQ=60)
                                             TATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTA                                                  >  SRR4302542.2657351/1‑94 (MQ=60)
                                              ATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAG                                                 >  SRR4302542.6387516/1‑94 (MQ=60)
                                              ATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAG                                                 <  SRR4302542.3650335/94‑1 (MQ=60)
                                               TCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGC                                                <  SRR4302542.3498574/94‑1 (MQ=60)
                                               TCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGC                                                <  SRR4302542.1251811/94‑1 (MQ=60)
                                                CTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCC                                               >  SRR4302542.6387876/1‑94 (MQ=60)
                                                 TTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCG                                              >  SRR4302542.57582/1‑94 (MQ=60)
                                                 TTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCG                                              <  SRR4302542.5735547/94‑1 (MQ=60)
                                                 TTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCG                                              <  SRR4302542.6114947/94‑1 (MQ=60)
                                                  TGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGC                                             <  SRR4302542.5366219/94‑1 (MQ=60)
                                                  TGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGC                                             >  SRR4302542.3813569/1‑94 (MQ=60)
                                                  TGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGC                                             >  SRR4302542.3872626/1‑94 (MQ=60)
                                                   GAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCG                                            >  SRR4302542.7098988/1‑94 (MQ=60)
                                                    AATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGA                                           >  SRR4302542.2989440/1‑94 (MQ=60)
                                                     ATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAT                                          >  SRR4302542.5709157/1‑94 (MQ=60)
                                                     ATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGAT                                          >  SRR4302542.966907/1‑94 (MQ=60)
                                                      TCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATT                                         <  SRR4302542.3071801/94‑1 (MQ=60)
                                                      TCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATT                                         >  SRR4302542.1571916/1‑94 (MQ=60)
                                                       CATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTT                                        >  SRR4302542.225526/1‑94 (MQ=60)
                                                       CATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTT                                        >  SRR4302542.2251135/1‑94 (MQ=60)
                                                        ATTAAGGTTAAATACATACGCAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTT                                       >  SRR4302542.7391945/1‑94 (MQ=60)
                                                         TTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTG                                      <  SRR4302542.3340453/94‑1 (MQ=60)
                                                         TTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTG                                      <  SRR4302542.6062431/94‑1 (MQ=60)
                                                          TAAGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGA                                     >  SRR4302542.490114/1‑94 (MQ=60)
                                                            AGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATA                                   >  SRR4302542.966723/1‑94 (MQ=60)
                                                            AGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATA                                   >  SRR4302542.7114721/1‑94 (MQ=60)
                                                            AGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATA                                   >  SRR4302542.2115208/1‑94 (MQ=60)
                                                            AGGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATA                                   >  SRR4302542.2419611/1‑94 (MQ=60)
                                                             GGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATAT                                  <  SRR4302542.1855125/94‑1 (MQ=60)
                                                             GGTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATAT                                  <  SRR4302542.4895847/94‑1 (MQ=60)
                                                             GGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATAT                                  >  SRR4302542.7128885/1‑94 (MQ=60)
                                                              GTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATC                                 <  SRR4302542.4299966/94‑1 (MQ=60)
                                                              GTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATC                                 >  SRR4302542.516239/1‑94 (MQ=60)
                                                              GTTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATC                                 <  SRR4302542.2857791/94‑1 (MQ=60)
                                                               TTAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCG                                <  SRR4302542.5367860/94‑1 (MQ=60)
                                                                TAAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGG                               <  SRR4302542.1095363/94‑1 (MQ=60)
                                                                 AAATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGG                              >  SRR4302542.3000260/1‑94 (MQ=60)
                                                                  AATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGC                             <  SRR4302542.4145232/94‑1 (MQ=60)
                                                                  AATACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGC                             >  SRR4302542.4153547/1‑94 (MQ=60)
                                                                   ATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCC                            >  SRR4302542.1035321/1‑94 (MQ=60)
                                                                    TACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCA                           <  SRR4302542.999728/94‑1 (MQ=60)
                                                                    TACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCA                           <  SRR4302542.3387764/94‑1 (MQ=60)
                                                                    TACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCA                           >  SRR4302542.5592922/1‑94 (MQ=60)
                                                                    TACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCA                           <  SRR4302542.6346477/94‑1 (MQ=60)
                                                                     ACATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCAT                          >  SRR4302542.2856173/1‑94 (MQ=60)
                                                                       ATACGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTA                        >  SRR4302542.1307806/1‑94 (MQ=60)
                                                                          CGTAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAA                     >  SRR4302542.5732764/1‑94 (MQ=60)
                                                                            TAAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAA                   <  SRR4302542.6194987/94‑1 (MQ=60)
                                                                             AAGTTTGGCTCGTAATGCTTACTGGGACGCTAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAC                  >  SRR4302542.2323630/1‑94 (MQ=60)
                                                                             AAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAC                  <  SRR4302542.1209342/94‑1 (MQ=60)
                                                                             AAGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAC                  >  SRR4302542.1930558/1‑94 (MQ=60)
                                                                             AAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAAC                  <  SRR4302542.2173436/94‑1 (MQ=60)
                                                                              AGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACG                 <  SRR4302542.2793873/94‑1 (MQ=60)
                                                                              AGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACG                 >  SRR4302542.3456310/1‑94 (MQ=60)
                                                                              AGTTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGAAATCGGGCCATTAAAAAACG                 <  SRR4302542.4961876/94‑1 (MQ=60)
                                                                              AGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACG                 >  SRR4302542.1935738/1‑94 (MQ=60)
                                                                              AGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACG                 <  SRR4302542.6504543/94‑1 (MQ=60)
                                                                                TTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTC               >  SRR4302542.6078276/1‑94 (MQ=60)
                                                                                TTTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTC               <  SRR4302542.4427321/94‑1 (MQ=60)
                                                                                 TTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCT              <  SRR4302542.2367324/94‑1 (MQ=60)
                                                                                 TTGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCT              <  SRR4302542.6364102/94‑1 (MQ=60)
                                                                                  TGGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTC             >  SRR4302542.4515300/1‑94 (MQ=60)
                                                                                  TGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTC             >  SRR4302542.139981/1‑94 (MQ=60)
                                                                                   GGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCC            >  SRR4302542.4528628/1‑94 (MQ=60)
                                                                                   GGCTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCC            <  SRR4302542.728928/94‑1 (MQ=60)
                                                                                   GGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCC            <  SRR4302542.493877/94‑1 (MQ=60)
                                                                                     CTCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCAC          <  SRR4302542.3300370/94‑1 (MQ=60)
                                                                                      TCGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACT         <  SRR4302542.6054044/94‑1 (MQ=60)
                                                                                       CGTAATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTT        <  SRR4302542.2005963/94‑1 (MQ=60)
                                                                                        GTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTC       >  SRR4302542.4799372/1‑94 (MQ=60)
                                                                                          AATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTC     >  SRR4302542.4789966/1‑94 (MQ=60)
                                                                                          AATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTC     >  SRR4302542.1379328/1‑94 (MQ=60)
                                                                                           ATGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCT    >  SRR4302542.2755422/1‑94 (MQ=60)
                                                                                           ATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCT    <  SRR4302542.4283472/94‑1 (MQ=60)
                                                                                            TGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTA   >  SRR4302542.2377022/1‑94 (MQ=60)
                                                                                            TGCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTA   <  SRR4302542.2743787/94‑1 (MQ=60)
                                                                                             GCTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTAA  >  SRR4302542.4144783/1‑94 (MQ=60)
                                                                                             ACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTAA  >  SRR4302542.421286/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATTATCAGAATGAAAAAGAGAGTTATATTGTCACGTTTAACCAGTATCTTGAATCATTAAGGTTAAATACATACGTAAGTTTGGCTCGTAATACTTACTGGGACGCCAGCAGTAATGTGAATTATTCATTATCACTTAGCCGCGATTTTGATATCGGGCCATTAAAAAACGTCTCCACTTCTCTAA  >  NC_000913/3186832‑3187018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑