Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,209,171 T→C Y142H (TAT→CAT)  dnaG → DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,209,1710TC83.9% 585.1 / 105.1 261Y142H (TAT→CAT) dnaGDNA primase
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base C (119/100);  total (140/121)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

Only 100 of 264 total aligned reads displayed.

TCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTT  >  NC_000913/3209080‑3209263
                                                                                           |                                                                                            
  agagGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATc                                                                                           <  2:6331975/93‑1 (MQ=255)
  agagGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATc                                                                                           <  1:2666299/93‑1 (MQ=255)
    agGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTg                                                                                         <  1:3033939/93‑1 (MQ=255)
    agGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTg                                                                                         <  1:5849332/93‑1 (MQ=255)
    agGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTg                                                                                         <  2:6632444/93‑1 (MQ=255)
      gCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGa                                                                                       <  2:3057311/93‑1 (MQ=255)
      gCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGa                                                                                       <  2:4866176/93‑1 (MQ=255)
         aaCGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGaaaa                                                                                    >  1:63144/1‑93 (MQ=255)
          aCGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGaaaaa                                                                                   >  1:5459690/1‑93 (MQ=255)
           cGCTTTATCAGTTGATGGATGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAAc                                                                                  <  2:40320/93‑1 (MQ=255)
           cGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAAc                                                                                  >  2:1650028/1‑93 (MQ=255)
            gCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAAcg                                                                                 <  1:6563682/93‑1 (MQ=255)
             cTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAAcgc                                                                                <  2:3244538/93‑1 (MQ=255)
             cTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAAcgc                                                                                >  1:3081294/1‑93 (MQ=255)
                tATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGa                                                                             <  1:2619905/93‑1 (MQ=255)
                tATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGa                                                                             >  2:4215659/1‑93 (MQ=255)
                 aTCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGAt                                                                            <  1:932777/93‑1 (MQ=255)
                 aTCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAt                                                                            >  1:3523832/1‑93 (MQ=255)
                 aTCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAt                                                                            <  1:5545062/93‑1 (MQ=255)
                  tCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAtt                                                                           <  1:4128796/93‑1 (MQ=255)
                  tCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAtt                                                                           >  1:4461001/1‑93 (MQ=255)
                    aGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTaa                                                                         <  2:5424617/93‑1 (MQ=255)
                    aGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTaa                                                                         <  2:3616096/93‑1 (MQ=255)
                      ttGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGc                                                                       <  1:7384041/93‑1 (MQ=255)
                      ttGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGc                                                                       <  2:1716831/93‑1 (MQ=255)
                        gATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCa                                                                     <  2:2553562/93‑1 (MQ=255)
                          tGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACg                                                                   <  2:2045287/93‑1 (MQ=255)
                          tGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACg                                                                   >  1:1698334/1‑93 (MQ=255)
                           ggACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGa                                                                  <  1:6635573/93‑1 (MQ=255)
                            gACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAg                                                                 <  1:7232406/93‑1 (MQ=255)
                            gACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAg                                                                 >  2:3773178/1‑93 (MQ=255)
                            gACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAg                                                                 >  1:4877184/1‑93 (MQ=255)
                             aCGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCTACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAgg                                                                <  2:6819971/93‑1 (MQ=255)
                             aCGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAgg                                                                >  2:2443334/1‑93 (MQ=255)
                                gTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTa                                                             >  2:3411988/1‑93 (MQ=255)
                                gTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTa                                                             >  1:5992177/1‑93 (MQ=255)
                                   tGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcg                                                          >  2:1951931/1‑93 (MQ=255)
                                   tGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcg                                                          >  2:2992155/1‑93 (MQ=255)
                                   tGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcg                                                          >  1:3787669/1‑93 (MQ=255)
                                    gAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgc                                                         <  1:1371781/93‑1 (MQ=255)
                                     aaTACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgct                                                        <  1:5142893/93‑1 (MQ=255)
                                     aaTACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgct                                                        <  2:513454/93‑1 (MQ=255)
                                      aTACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgctc                                                       >  1:3198155/1‑93 (MQ=255)
                                        aCGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgctcgc                                                     >  1:2868590/1‑93 (MQ=255)
                                          gTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCtt                                                   >  2:4613270/1‑93 (MQ=255)
                                          gTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCtt                                                   <  1:2155635/93‑1 (MQ=255)
                                           ttttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCttt                                                  <  1:4049929/93‑1 (MQ=255)
                                           ttttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCttt                                                  >  2:1032424/1‑93 (MQ=255)
                                             ttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGc                                                <  1:6113741/93‑1 (MQ=255)
                                             ttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGc                                                <  2:1251967/93‑1 (MQ=255)
                                               aCCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGa                                              >  1:4335517/1‑93 (MQ=255)
                                                ccAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAt                                             >  1:2143236/1‑93 (MQ=255)
                                                 caacaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAtt                                            <  1:1954185/93‑1 (MQ=255)
                                                 caacaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAtt                                            >  2:1158622/1‑93 (MQ=255)
                                                  aacaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTg                                           >  1:5569084/1‑93 (MQ=255)
                                                   acaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTgg                                          >  1:5735758/1‑93 (MQ=255)
                                                   acaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTgg                                          <  1:3736728/93‑1 (MQ=255)
                                                    caaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGt                                         <  2:3904816/93‑1 (MQ=255)
                                                      aTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGttt                                       >  2:5730608/1‑93 (MQ=255)
                                                        cTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTg                                     >  1:1158954/1‑93 (MQ=255)
                                                        cTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTg                                     >  1:7004024/1‑93 (MQ=255)
                                                         tttACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTgc                                    <  2:3835314/93‑1 (MQ=255)
                                                         tttACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTgc                                    >  2:2654141/1‑93 (MQ=255)
                                                          ttACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTgcg                                   >  2:2726092/1‑93 (MQ=255)
                                                              aacaacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccc                               >  1:2598186/1‑93 (MQ=255)
                                                               acaacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGccccc                              >  2:6092339/1‑93 (MQ=255)
                                                               acaacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGccccc                              >  2:896147/1‑93 (MQ=255)
                                                               acaacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGccccc                              >  1:143427/1‑93 (MQ=255)
                                                               acaacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGccccc                              >  1:6836741/1‑93 (MQ=255)
                                                                cacccTGTTGCCCCGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccccc                             <  1:6436182/90‑1 (MQ=255)
                                                                caacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccccc                             <  2:2617282/93‑1 (MQ=255)
                                                                caacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccccc                             <  2:1469107/93‑1 (MQ=255)
                                                                 aacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCg                            >  2:2988706/1‑93 (MQ=255)
                                                                 aacCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCg                            >  1:2498411/1‑93 (MQ=255)
                                                                  acCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccccccg                           >  2:4353969/1‑91 (MQ=255)
                                                                  acCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCgg                           >  2:291595/1‑93 (MQ=255)
                                                                  acCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCgg                           >  2:2260332/1‑93 (MQ=255)
                                                                   ccTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGc                          <  2:7047031/93‑1 (MQ=255)
                                                                    cTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCt                         >  1:1087874/1‑93 (MQ=255)
                                                                     tGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTg                        <  2:774862/93‑1 (MQ=255)
                                                                     tGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTg                        >  2:4800965/1‑93 (MQ=255)
                                                                      gTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTgg                       >  2:2850994/1‑93 (MQ=255)
                                                                      gTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTgg                       <  2:5055038/93‑1 (MQ=255)
                                                                       ttGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTggg                      <  1:2567304/93‑1 (MQ=255)
                                                                       ttGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTggg                      >  2:319521/1‑93 (MQ=255)
                                                                         gCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGAc                    >  2:5542997/1‑93 (MQ=255)
                                                                          ccACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACa                   >  2:6870327/1‑93 (MQ=255)
                                                                           cACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACaa                  <  2:2119584/93‑1 (MQ=255)
                                                                           cACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACaa                  <  2:5997336/93‑1 (MQ=255)
                                                                            aCGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAAc                 >  2:2379659/1‑93 (MQ=255)
                                                                               tCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTc              >  2:4748900/1‑93 (MQ=255)
                                                                               tCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTc              >  1:2955206/1‑93 (MQ=255)
                                                                               tCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTc              >  1:5344188/1‑93 (MQ=255)
                                                                                cTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTcc             <  1:1847557/93‑1 (MQ=255)
                                                                                cTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTcc             <  1:2309722/93‑1 (MQ=255)
                                                                                 tGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCt            <  2:6533868/93‑1 (MQ=255)
                                                                                 tGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCt            >  2:7473355/1‑93 (MQ=255)
                                                                                  gcgcgcCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTg           >  1:174285/1‑93 (MQ=255)
                                                                                    gcgcCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGaa         <  2:58305/93‑1 (MQ=255)
                                                                                        cAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACATCCTGAAGCgg     >  1:389523/1‑93 (MQ=255)
                                                                                           |                                                                                            
TCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTT  >  NC_000913/3209080‑3209263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  >  NC_000913/3209078‑3209264
                                                                                             |                                                                                             
CATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGC                                                                                               >  SRR4302542.5195202/1‑94 (MQ=60)
 ATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTA                                                                                              >  SRR4302542.3102699/1‑94 (MQ=60)
  TCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTAT                                                                                             <  SRR4302542.5172993/94‑1 (MQ=60)
  TCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCAT                                                                                             >  SRR4302542.3891257/1‑94 (MQ=60)
  TCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCAT                                                                                             <  SRR4302542.3762776/94‑1 (MQ=60)
   CAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATC                                                                                            >  SRR4302542.6205314/1‑94 (MQ=60)
   CAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATC                                                                                            <  SRR4302542.3167285/94‑1 (MQ=60)
    AGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCT                                                                                           <  SRR4302542.2692041/94‑1 (MQ=60)
      AGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGG                                                                                         <  SRR4302542.3062884/94‑1 (MQ=60)
      AGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGG                                                                                         <  SRR4302542.5906059/94‑1 (MQ=60)
      AGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGG                                                                                         <  SRR4302542.3810765/94‑1 (MQ=60)
         CAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAA                                                                                      >  SRR4302542.3026000/1‑94 (MQ=60)
          AAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAA                                                                                     >  SRR4302542.63656/1‑94 (MQ=60)
           AACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAA                                                                                    >  SRR4302542.5512732/1‑94 (MQ=60)
             CGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACG                                                                                  <  SRR4302542.2979118/94‑1 (MQ=60)
             CGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACG                                                                                  <  SRR4302542.664787/94‑1 (MQ=60)
              GCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGC                                                                                 >  SRR4302542.3110636/1‑94 (MQ=60)
              GCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGC                                                                                 >  SRR4302542.5697226/1‑94 (MQ=60)
              GCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGC                                                                                 <  SRR4302542.6628367/94‑1 (MQ=60)
               CTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCG                                                                                >  SRR4302542.3701181/1‑94 (MQ=60)
                TTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGG                                                                               <  SRR4302542.2660083/94‑1 (MQ=60)
                  TATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGAT                                                                             <  SRR4302542.2645223/94‑1 (MQ=60)
                  TATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAT                                                                             >  SRR4302542.3075371/1‑94 (MQ=60)
                  TATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAT                                                                             <  SRR4302542.5550375/94‑1 (MQ=60)
                  TATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGAT                                                                             >  SRR4302542.3556760/1‑94 (MQ=60)
                   ATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATT                                                                            <  SRR4302542.940807/94‑1 (MQ=60)
                   ATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATT                                                                            >  SRR4302542.258631/1‑94 (MQ=60)
                   ATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATT                                                                            >  SRR4302542.4504759/1‑94 (MQ=60)
                   ATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATT                                                                            >  SRR4302542.1555659/1‑94 (MQ=60)
                   ATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATT                                                                            <  SRR4302542.5598948/94‑1 (MQ=60)
                    TCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTA                                                                           <  SRR4302542.4169471/94‑1 (MQ=60)
                      AGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAG                                                                         <  SRR4302542.5719005/94‑1 (MQ=60)
                      AGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAG                                                                         <  SRR4302542.2101235/94‑1 (MQ=60)
                       GTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGC                                                                        >  SRR4302542.5803473/1‑94 (MQ=60)
                        TTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCC                                                                       <  SRR4302542.7456328/94‑1 (MQ=60)
                        TTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCC                                                                       >  SRR4302542.558233/1‑94 (MQ=60)
                        TTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCC                                                                       <  SRR4302542.4221527/94‑1 (MQ=60)
                        TTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCC                                                                       <  SRR4302542.2308628/94‑1 (MQ=60)
                        NNGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCC                                                                       <  SRR4302542.89204/94‑1 (MQ=60)
                          GATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCAC                                                                     <  SRR4302542.2165354/94‑1 (MQ=60)
                          GATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCAC                                                                     >  SRR4302542.6449894/1‑94 (MQ=60)
                           ATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACG                                                                    >  SRR4302542.1753013/1‑94 (MQ=60)
                           ATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACG                                                                    >  SRR4302542.1713048/1‑94 (MQ=60)
                           ATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACG                                                                    >  SRR4302542.2510238/1‑94 (MQ=60)
                             GGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAG                                                                  >  SRR4302542.2651996/1‑94 (MQ=60)
                             GGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAG                                                                  >  SRR4302542.4924649/1‑94 (MQ=60)
                             GGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAG                                                                  <  SRR4302542.6700986/94‑1 (MQ=60)
                              GACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGG                                                                 <  SRR4302542.7303346/94‑1 (MQ=60)
                                 GGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTA                                                              >  SRR4302542.5115652/1‑94 (MQ=60)
                                 GGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTA                                                              >  SRR4302542.5814740/1‑94 (MQ=60)
                                 GGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTA                                                              <  SRR4302542.6974849/94‑1 (MQ=60)
                                 GGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTA                                                              >  SRR4302542.6050217/1‑94 (MQ=60)
                                  GTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAT                                                             <  SRR4302542.4064521/94‑1 (MQ=60)
                                  GTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTAT                                                             >  SRR4302542.4706802/1‑94 (MQ=60)
                                   TCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATC                                                            >  SRR4302542.6802511/1‑94 (MQ=60)
                                    CTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCG                                                           <  SRR4302542.1729200/94‑1 (MQ=60)
                                    CTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCG                                                           >  SRR4302542.3825122/1‑94 (MQ=60)
                                     TGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGC                                                          >  SRR4302542.2607102/1‑94 (MQ=60)
                                      GAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCT                                                         >  SRR4302542.4466513/1‑94 (MQ=60)
                                      GAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCT                                                         >  SRR4302542.1596841/1‑94 (MQ=60)
                                      GAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCT                                                         <  SRR4302542.1383638/94‑1 (MQ=60)
                                       AATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTC                                                        >  SRR4302542.3228438/1‑94 (MQ=60)
                                       AATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTC                                                        <  SRR4302542.5192882/94‑1 (MQ=60)
                                        ATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCG                                                       >  SRR4302542.3033755/1‑94 (MQ=60)
                                         TACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGC                                                      >  SRR4302542.2896148/1‑94 (MQ=60)
                                            GTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTT                                                   <  SRR4302542.2174204/94‑1 (MQ=60)
                                            GTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTT                                                   >  SRR4302542.2643464/1‑94 (MQ=60)
                                             TTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTG                                                  <  SRR4302542.6929182/94‑1 (MQ=60)
                                             TTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTG                                                  <  SRR4302542.4089876/94‑1 (MQ=60)
                                              TTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGC                                                 >  SRR4302542.3286748/1‑94 (MQ=60)
                                               TTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCG                                                <  SRR4302542.6172904/94‑1 (MQ=60)
                                                TACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGA                                               >  SRR4302542.4378122/1‑94 (MQ=60)
                                                TACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGA                                               >  SRR4302542.5467192/1‑94 (MQ=60)
                                                 ACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAT                                              >  SRR4302542.2161723/1‑94 (MQ=60)
                                                 ACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAT                                              >  SRR4302542.120371/1‑94 (MQ=60)
                                                 ACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAT                                              <  SRR4302542.2242770/94‑1 (MQ=60)
                                                 ACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAT                                              >  SRR4302542.1824585/1‑94 (MQ=60)
                                                 ACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGAT                                              >  SRR4302542.868178/1‑94 (MQ=60)
                                                  CCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATT                                             <  SRR4302542.1883285/94‑1 (MQ=60)
                                                  CCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATT                                             >  SRR4302542.2396282/1‑94 (MQ=60)
                                                   CAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTG                                            <  SRR4302542.3720548/94‑1 (MQ=60)
                                                   CAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTG                                            <  SRR4302542.1971087/94‑1 (MQ=60)
                                                   CAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTG                                            >  SRR4302542.5623199/1‑94 (MQ=60)
                                                    AACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGG                                           >  SRR4302542.5791453/1‑94 (MQ=60)
                                                     ACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGT                                          <  SRR4302542.3773719/94‑1 (MQ=60)
                                                         TCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTG                                      >  SRR4302542.7072824/1‑94 (MQ=60)
                                                         TCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTG                                      <  SRR4302542.4573380/94‑1 (MQ=60)
                                                         TCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTG                                      <  SRR4302542.5407774/94‑1 (MQ=60)
                                                         TCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTG                                      <  SRR4302542.3552254/94‑1 (MQ=60)
                                                         TCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTG                                      >  SRR4302542.1168889/1‑94 (MQ=60)
                                                           TTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCG                                    <  SRR4302542.7324746/94‑1 (MQ=60)
                                                             TACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCC                                  >  SRR4302542.7415039/1‑94 (MQ=60)
                                                              ACAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCC                                 >  SRR4302542.6510346/1‑94 (MQ=60)
                                                               CAACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCC                                >  SRR4302542.2623330/1‑94 (MQ=60)
                                                                AACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCC                               >  SRR4302542.144625/1‑94 (MQ=60)
                                                                AACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCC                               >  SRR4302542.6903922/1‑94 (MQ=60)
                                                                AACAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCC                               >  SRR4302542.1992422/1‑94 (MQ=60)
                                                                  CACCCTGTTGCCCCGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCG                             <  SRR4302542.6499584/94‑1 (MQ=60)
                                                                  CAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCG                             >  SRR4302542.2522637/1‑94 (MQ=60)
                                                                  CAACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCG                             <  SRR4302542.6390224/94‑1 (MQ=60)
                                                                   AACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGG                            <  SRR4302542.5618608/94‑1 (MQ=60)
                                                                   AACCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGG                            >  SRR4302542.682264/1‑94 (MQ=60)
                                                                     CCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCT                          <  SRR4302542.1890219/94‑1 (MQ=60)
                                                                     CCTGTTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCT                          >  SRR4302542.1097201/1‑94 (MQ=60)
                                                                      CTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTG                         <  SRR4302542.1297528/94‑1 (MQ=60)
                                                                         TTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGA                      <  SRR4302542.2592183/94‑1 (MQ=60)
                                                                         TTGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGA                      <  SRR4302542.5376302/94‑1 (MQ=60)
                                                                          TGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGAC                     >  SRR4302542.6065482/1‑94 (MQ=60)
                                                                          TGCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGAC                     <  SRR4302542.6010078/94‑1 (MQ=60)
                                                                           GCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACA                    >  SRR4302542.2727706/1‑94 (MQ=60)
                                                                           GCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACA                    <  SRR4302542.2606483/94‑1 (MQ=60)
                                                                           GCCACGTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACA                    <  SRR4302542.4295743/94‑1 (MQ=60)
                                                                             CACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAAC                  <  SRR4302542.1147603/94‑1 (MQ=60)
                                                                                GTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTC               >  SRR4302542.5396102/1‑94 (MQ=60)
                                                                                GTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTC               <  SRR4302542.6559512/94‑1 (MQ=60)
                                                                                GTCTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTC               >  SRR4302542.2983491/1‑94 (MQ=60)
                                                                                  CTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCT             <  SRR4302542.1863543/94‑1 (MQ=60)
                                                                                  CTGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCT             <  SRR4302542.2329552/94‑1 (MQ=60)
                                                                                   TGCGCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTG            >  SRR4302542.175754/1‑94 (MQ=60)
                                                                                     CGCGCCAGCATCGGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAA          <  SRR4302542.4015622/94‑1 (MQ=60)
                                                                                      GCGCCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAG         <  SRR4302542.7106483/94‑1 (MQ=60)
                                                                                         CCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGG      >  SRR4302542.1388133/1‑94 (MQ=60)
                                                                                         CCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGG      >  SRR4302542.3852267/1‑94 (MQ=60)
                                                                                         CCAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACATCCTGAAGCGG      >  SRR4302542.392893/1‑94 (MQ=60)
                                                                                          CAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGT     >  SRR4302542.1324666/1‑94 (MQ=60)
                                                                                          CAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGT     >  SRR4302542.4628042/1‑94 (MQ=60)
                                                                                          CAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGT     <  SRR4302542.4144896/94‑1 (MQ=60)
                                                                                          CAGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGT     <  SRR4302542.2877271/94‑1 (MQ=60)
                                                                                           AGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTT    >  SRR4302542.3090090/1‑94 (MQ=60)
                                                                                           AGCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTT    <  SRR4302542.4657760/94‑1 (MQ=60)
                                                                                            GCATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTT   <  SRR4302542.2169058/94‑1 (MQ=60)
                                                                                             TATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.607533/94‑1 (MQ=60)
                                                                                             CATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.7058542/94‑1 (MQ=60)
                                                                                             CATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.762348/94‑1 (MQ=60)
                                                                                             CATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.5364894/94‑1 (MQ=60)
                                                                                             CATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.5536282/94‑1 (MQ=60)
                                                                                             CATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  <  SRR4302542.5848024/94‑1 (MQ=60)
                                                                                             |                                                                                             
CATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTG  >  NC_000913/3209078‑3209264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑