Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,097 2 bp→GC coding (824‑825/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,0970CG99.8% 2051.3 / ‑5.0 528T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (276/251);  minor base T (1/0);  total (277/251)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01
*NC_0009133,221,0980GC99.2% 2041.8 / ‑4.6 528T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (273/250);  minor base A (4/0);  total (277/251)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 543 total aligned reads displayed.

CGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGG  >  NC_000913/3221007‑3221187
                                                                                          ||                                                                                         
cgacgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTg                                                                                       >  2:4981211/1‑96 (MQ=255)
  acgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCt                                                                                     <  2:4310705/96‑1 (MQ=255)
     cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt                                                                                  <  2:1649442/96‑1 (MQ=255)
     cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt                                                                                  >  1:12841845/1‑96 (MQ=255)
      tcgAATATACCCTGTTTGATGGCGAACGCGTGGGGCACAGCAGCGCCATTGAGCATTTGGCAATTGAAAAACTAACCAGCGCCAGCTTTGCTTTTa                                                                                 >  1:10600288/3‑96 (MQ=255)
       ggAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc                                                                                <  2:10358198/96‑1 (MQ=255)
        gAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACt                                                                               >  1:5583863/1‑96 (MQ=255)
        gAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACt                                                                               <  1:6370138/96‑1 (MQ=255)
         aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACCGCAGCCCCATTGATCATTTGGCAATTGAAAAACCGACCAGCGCCAGCTTTGCTTTTACTg                                                                              <  1:4003712/96‑1 (MQ=255)
          atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                             >  2:4064086/1‑96 (MQ=255)
          atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                             >  2:5451797/1‑96 (MQ=255)
           tataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTc                                                                            >  2:961545/1‑96 (MQ=255)
            ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCg                                                                           >  1:2651087/1‑96 (MQ=255)
              aCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa                                                                         >  1:8345101/1‑96 (MQ=255)
               cccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                        <  1:12460000/96‑1 (MQ=255)
               cccGGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                        >  2:10101323/1‑96 (MQ=255)
                    tttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                   >  1:1563890/1‑96 (MQ=255)
                    tttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                   <  1:566285/96‑1 (MQ=255)
                     ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                                  <  2:7689443/96‑1 (MQ=255)
                     ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                                  <  1:6009722/96‑1 (MQ=255)
                      tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgca                                                                 <  1:7573971/96‑1 (MQ=255)
                           gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                            <  2:12055471/96‑1 (MQ=255)
                            cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATg                                                           <  1:4841729/96‑1 (MQ=255)
                             gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg                                                          >  1:6836959/1‑96 (MQ=255)
                             gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg                                                          >  2:9783931/1‑96 (MQ=255)
                             gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg                                                          >  1:11659998/1‑96 (MQ=255)
                              aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                         >  2:10743551/1‑96 (MQ=255)
                              aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                         >  1:6216419/1‑96 (MQ=255)
                               aCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                        <  2:2843419/96‑1 (MQ=255)
                                cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                       <  1:9836944/96‑1 (MQ=255)
                                cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                       <  1:2783515/96‑1 (MQ=255)
                                 gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                      <  1:1303659/96‑1 (MQ=255)
                                 gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                      >  2:8493715/1‑96 (MQ=255)
                                  cgTGGTGCACAGCAGCGCCTTTGATCATTTGGCAATTGAAAAACTGACTAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                     >  2:12773246/1‑96 (MQ=255)
                                  cgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                     >  1:8984805/1‑96 (MQ=255)
                                   gtggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                    <  1:12086368/96‑1 (MQ=255)
                                     ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa                                                  >  1:2299966/1‑96 (MQ=255)
                                      gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                                 <  1:4538807/96‑1 (MQ=255)
                                      gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                                 <  1:2446327/96‑1 (MQ=255)
                                       tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                                <  2:6866603/96‑1 (MQ=255)
                                       tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                                <  2:2447424/96‑1 (MQ=255)
                                       tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                                <  2:7253873/96‑1 (MQ=255)
                                        gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATc                                               >  2:2612366/1‑96 (MQ=255)
                                        gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATc                                               <  1:9519118/96‑1 (MQ=255)
                                         cacaGCAGCGCCATTGATCATTTGGCAATTCAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAttc                                              <  1:10283590/96‑3 (MQ=255)
                                          acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATccc                                             >  1:6568772/1‑96 (MQ=255)
                                             gcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                          <  2:10212899/96‑1 (MQ=255)
                                              cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa                                         <  2:11228378/96‑1 (MQ=255)
                                               aacgcCATTGATCATTTGGCAATGGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAt                                        >  1:14333637/3‑96 (MQ=255)
                                                  gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt                                     >  2:6407473/1‑96 (MQ=255)
                                                  gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGATTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCATCAGAATCCCCTTATCtt                                     >  1:11354279/1‑96 (MQ=255)
                                                   ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt                                    <  2:5204726/96‑1 (MQ=255)
                                                   ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt                                    >  2:7070768/1‑96 (MQ=255)
                                                     aTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                                  <  1:471614/96‑1 (MQ=255)
                                                      ttGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                                 <  1:9174171/96‑1 (MQ=255)
                                                      ttGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                                 >  1:11008982/1‑96 (MQ=255)
                                                        gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                               >  1:13378760/1‑96 (MQ=255)
                                                        gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                               <  1:5266334/96‑1 (MQ=255)
                                                        gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                               >  2:14282697/1‑96 (MQ=255)
                                                         agcATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                              >  1:7365381/3‑96 (MQ=255)
                                                         aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCGGAATCCCCTTATCTTAACCATc                              >  2:11768452/1‑96 (MQ=255)
                                                         aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGGATCCCCTTATCTTTACCATc                              >  2:13970968/1‑96 (MQ=255)
                                                         aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                              <  2:12283308/96‑1 (MQ=255)
                                                          tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                             <  2:6774235/96‑1 (MQ=255)
                                                          tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                             <  2:13315226/96‑1 (MQ=255)
                                                          tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                             <  1:6751819/96‑1 (MQ=255)
                                                          tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                             >  1:348755/1‑96 (MQ=255)
                                                           cATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                            <  1:14005686/96‑1 (MQ=255)
                                                            aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAGAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                           >  2:317085/1‑96 (MQ=255)
                                                            aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                           >  1:1843847/1‑96 (MQ=255)
                                                            aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                           <  2:11601064/96‑1 (MQ=255)
                                                            aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGAAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                           >  1:7252951/1‑96 (MQ=255)
                                                             tttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGt                          <  1:822974/96‑1 (MQ=255)
                                                              ttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                         >  2:8371243/1‑96 (MQ=255)
                                                              ttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                         <  2:11027904/96‑1 (MQ=255)
                                                               tGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                        <  2:7383219/96‑1 (MQ=255)
                                                               tGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                        <  2:6568772/96‑1 (MQ=255)
                                                                 gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                      >  2:1834256/1‑96 (MQ=255)
                                                                 gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATg                      >  2:13769220/1‑96 (MQ=255)
                                                                   aaTTGAAAAACTGGCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                    <  1:802810/96‑1 (MQ=255)
                                                                   aaTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                    >  2:3822790/1‑96 (MQ=255)
                                                                    aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACg                   <  1:7119624/96‑1 (MQ=255)
                                                                     ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGc                  <  2:13611702/96‑1 (MQ=255)
                                                                      tGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCt                 >  1:3776742/1‑96 (MQ=255)
                                                                       gAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTg                >  2:9219306/1‑96 (MQ=255)
                                                                        ataaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGa               >  1:11091890/3‑96 (MQ=255)
                                                                         aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaa              >  2:3600099/1‑96 (MQ=255)
                                                                         aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGGCGAACAGCCGCAGCAATAGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaa              <  2:8211687/96‑1 (MQ=255)
                                                                          aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCATCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaaa             >  1:7752140/1‑96 (MQ=255)
                                                                          aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaaa             >  2:940813/1‑96 (MQ=255)
                                                                           aaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg            <  2:4598074/96‑1 (MQ=255)
                                                                             cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc          <  2:584663/96‑1 (MQ=255)
                                                                              tGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACg         <  2:148589/96‑1 (MQ=255)
                                                                                aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGcc       >  2:6082593/1‑96 (MQ=255)
                                                                                 ccAGCGCCAGCTTTGCTTTTACTGTCGATCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCa      <  1:12986947/96‑1 (MQ=255)
                                                                                  cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa     >  1:10028759/1‑96 (MQ=255)
                                                                                   aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAc    <  1:3270073/96‑1 (MQ=255)
                                                                                   aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAc    <  2:12990436/96‑1 (MQ=255)
                                                                                    gcgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACg   >  1:5008178/1‑96 (MQ=255)
                                                                                     cgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACgg  >  2:3436202/1‑96 (MQ=255)
                                                                                          ||                                                                                         
CGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGG  >  NC_000913/3221007‑3221187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 290 total aligned reads displayed.
CCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTG  >  NC_000913/3220997‑3221197
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CCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAG                                                                                                      <  SRR4302518.2223739/101‑1 (MQ=60)
     NNNCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                                 <  SRR4302518.7560507/101‑1 (MQ=60)
     CGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                                 <  SRR4302518.3253825/101‑1 (MQ=60)
     CGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                                 >  SRR4302518.6040448/1‑101 (MQ=60)
     CGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                                 >  SRR4302518.7352041/1‑101 (MQ=60)
      NNNACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                                <  SRR4302518.13535917/101‑1 (MQ=60)
      GTCACGACGCTGGAATATACCCTGTTTGATGGGGAAGCCGGGGTCCACAGCAGCGCCATTGTTCATTTGGCAATTAAAAAACTGACCAGCACCACCTTTGC                                                                                                >  SRR4302518.3890545/1‑101 (MQ=60)
      GTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                                <  SRR4302518.5794935/101‑1 (MQ=60)
      GTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                                >  SRR4302518.1070124/1‑101 (MQ=60)
          NNNCGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                            <  SRR4302518.13045571/101‑1 (MQ=60)
          NNNCGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                            <  SRR4302518.7869405/101‑1 (MQ=60)
           NNNGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCAAGCTTTGCTTTTA                                                                                           <  SRR4302518.14498829/101‑1 (MQ=60)
           GACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTA                                                                                           >  SRR4302518.5021827/1‑101 (MQ=60)
           GACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTNNN                                                                                           >  SRR4302518.8402334/1‑101 (MQ=60)
            NNNCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                                          <  SRR4302518.8029761/101‑1 (MQ=60)
            GCGCTGGAATATCCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                                          <  SRR4302518.1372101/101‑1 (MQ=60)
            ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                                          >  SRR4302518.5182417/1‑101 (MQ=60)
            ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTNNN                                                                                          >  SRR4302518.13363836/1‑101 (MQ=60)
             CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGGGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                                         >  SRR4302518.842236/1‑101 (MQ=60)
             CGCTAGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACT                                                                                         <  SRR4302518.6695362/101‑1 (MQ=60)
              NNNGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGACCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTG                                                                                        <  SRR4302518.12688130/101‑1 (MQ=60)
              GCTGGACAATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTG                                                                                        <  SRR4302518.1356379/101‑1 (MQ=60)
              GCGGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACCGCAGCCCCATTGATCATTTGGCAATTGAAAAACCGACCAGCGCCAGCTTTGCTTTTACTG                                                                                        <  SRR4302518.4195549/101‑1 (MQ=60)
               CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                                       <  SRR4302518.265393/101‑1 (MQ=60)
               CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                                       <  SRR4302518.3457787/101‑1 (MQ=60)
               CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACNNN                                                                                       >  SRR4302518.13438838/1‑101 (MQ=60)
                TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                                      <  SRR4302518.842957/101‑1 (MQ=60)
                TCGAATATACCCTGTTTGATGGCGAACGCGTGGGGCACAGCAGCGCCATTGAGCATTTGGCAATTGAAAAACTAACCAGCGCCAGCTTTGCTTTTACTNNN                                                                                      >  SRR4302518.11098221/1‑101 (MQ=60)
                GGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTNNN                                                                                      >  SRR4302518.9700166/1‑101 (MQ=60)
                 GGAATATACCCTGTTTGATGGCAAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTGGCTTTTACTGTCG                                                                                     >  SRR4302518.7282421/1‑101 (MQ=60)
                 GGAATATACCCTGTTTGAGGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                                     <  SRR4302518.6818587/101‑1 (MQ=60)
                  GAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGA                                                                                    <  SRR4302518.5401400/101‑1 (MQ=60)
                  GAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGA                                                                                    >  SRR4302518.5867202/1‑101 (MQ=60)
                  GAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCCA                                                                                    >  SRR4302518.7042382/1‑101 (MQ=60)
                   NNNATACGCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAA                                                                                   <  SRR4302518.13862452/101‑1 (MQ=60)
                   AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCGTTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCNNN                                                                                   >  SRR4302518.13616371/1‑101 (MQ=60)
                   AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCNNN                                                                                   >  SRR4302518.13592359/1‑101 (MQ=60)
                    NNNTACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                                  <  SRR4302518.13046296/101‑1 (MQ=60)
                    NNNTACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                                  <  SRR4302518.10079780/101‑1 (MQ=60)
                    ATATACCCTGTTTGATGGCGAACGCGTGTTGCACAGCTGCGCCATTGATCATTTGGCTATTGAAAAACTGACCAGAGCGAGCTTTGCTTTTACTGTCGNNN                                                                                  >  SRR4302518.12499440/1‑101 (MQ=60)
                    ATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGNNN                                                                                  >  SRR4302518.13953671/1‑101 (MQ=60)
                     TATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                                 <  SRR4302518.4986371/101‑1 (MQ=60)
                      ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAG                                                                                >  SRR4302518.2777804/1‑101 (MQ=60)
                       TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACNNN                                                                               >  SRR4302518.12059713/1‑101 (MQ=60)
                       NNNCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGC                                                                               <  SRR4302518.12907227/101‑1 (MQ=60)
                        TCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                              <  SRR4302518.7165646/101‑1 (MQ=60)
                        NNNCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                              <  SRR4302518.13512137/101‑1 (MQ=60)
                        ACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACANNN                                                                              >  SRR4302518.8755258/1‑101 (MQ=60)
                         CCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGNNN                                                                             >  SRR4302518.12633609/1‑101 (MQ=60)
                          GCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCNNN                                                                            >  SRR4302518.11790993/1‑101 (MQ=60)
                          CCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                            <  SRR4302518.6314646/101‑1 (MQ=60)
                          CCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGC                                                                            <  SRR4302518.771603/101‑1 (MQ=60)
                           NNNTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                           <  SRR4302518.7961569/101‑1 (MQ=60)
                           CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                           <  SRR4302518.6388538/101‑1 (MQ=60)
                             NNNTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAACAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGC                                                                         <  SRR4302518.10281322/101‑1 (MQ=60)
                             GTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGCACAGCCGCNNN                                                                         >  SRR4302518.8339505/1‑101 (MQ=60)
                             GTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTGGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGC                                                                         <  SRR4302518.4175877/101‑1 (MQ=60)
                              TTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCA                                                                        >  SRR4302518.1631598/1‑101 (MQ=60)
                              NNNGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCA                                                                        <  SRR4302518.8075054/101‑1 (MQ=60)
                               TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGNNN                                                                       >  SRR4302518.12455546/1‑101 (MQ=60)
                                TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCNNN                                                                      >  SRR4302518.14064735/1‑101 (MQ=60)
                                TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                                      >  SRR4302518.6605367/1‑101 (MQ=60)
                                TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAT                                                                      >  SRR4302518.5433811/1‑101 (MQ=60)
                                 gtgcgcgtaagcgtacttcacagccgcaCCATTGATCATGTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCCCAGCAATG                                                                     <  SRR4302518.52893/73‑1 (MQ=60)
                                 gagaGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                                     <  SRR4302518.5094812/97‑1 (MQ=60)
                                 GATGGCGCACCCTTGGTGCCCATCACCGCCATTCATCCTTTGGCAATTGAAAAACTGACCAGCGCCCGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                                     <  SRR4302518.4524469/101‑1 (MQ=52)
                                 GATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                                     <  SRR4302518.4808584/101‑1 (MQ=60)
                                  NNNGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                                    <  SRR4302518.10155236/101‑1 (MQ=60)
                                  ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                                    <  SRR4302518.7124176/101‑1 (MQ=60)
                                  AGGGCGAACGCGTGGTGCACAGCAGCGCCATTTATCATTTGGCAATTGAAAAACTGACCAGGGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAANNN                                                                    >  SRR4302518.9419562/1‑101 (MQ=60)
                                   TGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATNNN                                                                   >  SRR4302518.11779472/1‑101 (MQ=60)
                                    NNNGAACGCGTGGTGCACAGCAGCGCTATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                                  <  SRR4302518.7646891/101‑1 (MQ=60)
                                    NNNGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGTCGCAGCAATGGTC                                                                  <  SRR4302518.11469799/101‑1 (MQ=60)
                                    GGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                                  >  SRR4302518.5392727/1‑101 (MQ=60)
                                     NNNAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                                 <  SRR4302518.10307634/101‑1 (MQ=60)
                                     GCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGNNN                                                                 >  SRR4302518.15095097/1‑101 (MQ=60)
                                     GAGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGNNN                                                                 >  SRR4302518.10745783/1‑101 (MQ=60)
                                      CGAACGCGTGGTGCACAGCAGCGCCATTGGTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCCTTTACTGTCGAACAGCAGGAGCAATGGTNNN                                                                >  SRR4302518.14764439/1‑101 (MQ=60)
                                      CGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                                <  SRR4302518.474954/101‑1 (MQ=60)
                                       NNNCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTGCTGTCGAACAGCCGCAGCAATGGTCAGC                                                               <  SRR4302518.11695578/101‑1 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCNNN                                                               >  SRR4302518.11544042/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCNNN                                                               >  SRR4302518.12208895/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCNNN                                                               >  SRR4302518.14269616/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCNNN                                                               >  SRR4302518.8684069/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                               >  SRR4302518.6904753/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                               >  SRR4302518.3536261/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                               >  SRR4302518.504911/1‑101 (MQ=60)
                                       GAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                               >  SRR4302518.7198618/1‑101 (MQ=60)
                                       CAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCNNN                                                               >  SRR4302518.9288983/1‑101 (MQ=60)
                                        tacgcctggtccactgccGCGCCATTGCTCATTTAGCAATTGAAAAACTGACCCGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                              <  SRR4302518.5619658/83‑1 (MQ=60)
                                        NNNGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                              <  SRR4302518.12661197/101‑1 (MQ=60)
                                        AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                              <  SRR4302518.2735887/101‑1 (MQ=60)
                                        AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                              >  SRR4302518.6533437/1‑101 (MQ=60)
                                        AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCA                                                              <  SRR4302518.7060873/101‑1 (MQ=60)
                                         ACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAACTGAAAAACTGACCAGCGCCCGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAG                                                             <  SRR4302518.1885320/101‑1 (MQ=60)
                                          CGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGA                                                            <  SRR4302518.4159387/101‑1 (MQ=60)
                                           GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                           <  SRR4302518.5153558/101‑1 (MQ=60)
                                           GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                           >  SRR4302518.5926367/1‑101 (MQ=60)
                                           GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                           <  SRR4302518.2564464/101‑1 (MQ=60)
                                            CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGNNN                                                          >  SRR4302518.14795480/1‑101 (MQ=60)
                                            CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGNNN                                                          >  SRR4302518.11555822/1‑101 (MQ=60)
                                            CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                                          >  SRR4302518.2385500/1‑101 (MQ=60)
                                              TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAANNN                                                        >  SRR4302518.7979368/1‑101 (MQ=60)
                                              NNNTGCACAGCAGCGCCATTGATCATTTGGCAATTCAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATTC                                                        <  SRR4302518.10768833/101‑1 (MQ=60)
                                               GGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                                       >  SRR4302518.2411820/1‑101 (MQ=60)
                                                GTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAGCCCC                                                      >  SRR4302518.6180158/1‑101 (MQ=60)
                                                 NNNACAGCAGCGCCATTGATCATTTGGCTATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                                     <  SRR4302518.10595911/101‑1 (MQ=60)
                                                  cccccGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTT                                                    <  SRR4302518.4501826/96‑1 (MQ=60)
                                                  NNNCAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCAT                                                    <  SRR4302518.11195277/101‑1 (MQ=60)
                                                  GCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCNNN                                                    >  SRR4302518.8944340/1‑101 (MQ=60)
                                                   CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTA                                                   <  SRR4302518.3527985/101‑1 (MQ=60)
                                                   CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCACAATCCCCTTA                                                   >  SRR4302518.4948234/1‑101 (MQ=60)
                                                    CCAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                                  <  SRR4302518.5874992/101‑1 (MQ=60)
                                                    ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                                  >  SRR4302518.6906191/1‑101 (MQ=60)
                                                    ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                                  >  SRR4302518.2577388/1‑101 (MQ=60)
                                                    ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTNNN                                                  >  SRR4302518.8583499/1‑101 (MQ=60)
                                                    ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGCCAGCAGAATCCCCTTAT                                                  >  SRR4302518.2813696/1‑101 (MQ=60)
                                                     CAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCGGCAATGGTCAGCAGAATCCCCTTNNN                                                 >  SRR4302518.9585783/1‑101 (MQ=60)
                                                     CAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTNNN                                                 >  SRR4302518.13362521/1‑101 (MQ=60)
                                                     CAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                                 >  SRR4302518.2440365/1‑101 (MQ=60)
                                                     CAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                                 <  SRR4302518.2656705/101‑1 (MQ=60)
                                                      AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                                >  SRR4302518.7106479/1‑101 (MQ=60)
                                                      AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                                <  SRR4302518.7041349/101‑1 (MQ=60)
                                                      AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                                >  SRR4302518.3905930/1‑101 (MQ=60)
                                                      AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTAAGCAGAATCCCCTTATCT                                                >  SRR4302518.7186650/1‑101 (MQ=60)
                                                      AGCAGCGCCATTGAACATTTTGCAATTGAAAAACTGACCAGCGCCAGCTTTGCCTTTACTGTCCAAAAGCCGCAGCAAatggaaaccgaaaaccctttnnn                                                >  SRR4302518.10930101/1‑78 (MQ=47)
                                                       GCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTT                                               <  SRR4302518.2471522/101‑1 (MQ=60)
                                                        NNNCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTT                                              <  SRR4302518.7668618/101‑1 (MQ=60)
                                                        CAGCGCCATTGATCATTTGGCGATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTAATCNNN                                              >  SRR4302518.13463550/1‑101 (MQ=60)
                                                        CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCATCAATGGTCAGCAGAATCCCCTTATCNNN                                              >  SRR4302518.10776566/1‑101 (MQ=60)
                                                         AACGCCATTGATCATTTGGCAATTGAAAAACTGACCAGAGCCAGATTTGCTTTTACTGTCGATCAGCCGCAGCAATGGTTAGCAGAATCatcttatcgtga                                             >  SRR4302518.1648246/1‑89 (MQ=60)
                                                         AACGCCATTGATCATTTGGCAATGGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTNNN                                             >  SRR4302518.14996913/1‑101 (MQ=60)
                                                          NNNCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                            <  SRR4302518.8754172/101‑1 (MQ=60)
                                                          GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                            >  SRR4302518.3871499/1‑101 (MQ=60)
                                                          GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                            <  SRR4302518.2790987/101‑1 (MQ=60)
                                                          GCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                            <  SRR4302518.490211/101‑1 (MQ=60)
                                                           nnngATTGATCATTTGGCAATTGAAAAACTGACCAGCACCAGCTTTGCGTTTACTGCCTAACAGCCGCAGCAATGTTCAGCAGAACCCCCTTATCTTTACC                                           <  SRR4302518.7585770/97‑1 (MQ=60)
                                                            GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGATTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCATCAGAATCCCCTTATCTTTANNN                                          >  SRR4302518.11886401/1‑101 (MQ=60)
                                                             CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAT                                         >  SRR4302518.6122558/1‑101 (MQ=60)
                                                             CAATTGATCATTTGGCAATTGAAAAACTGCCCAGCGCCAGATTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACNNN                                         >  SRR4302518.12635806/1‑101 (MQ=60)
                                                               ATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                                       <  SRR4302518.7104193/101‑1 (MQ=60)
                                                               ATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCANNN                                       >  SRR4302518.14889121/1‑101 (MQ=60)
                                                                TTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATNNN                                      >  SRR4302518.11524348/1‑101 (MQ=60)
                                                                TTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                                      <  SRR4302518.2938549/101‑1 (MQ=60)
                                                                NNNATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTG                                      <  SRR4302518.14646515/101‑1 (MQ=60)
                                                                 TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGG                                     >  SRR4302518.2989914/1‑101 (MQ=60)
                                                                 TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGG                                     >  SRR4302518.986225/1‑101 (MQ=60)
                                                                 TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCNNN                                     >  SRR4302518.10159937/1‑101 (MQ=60)
                                                                 TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGGCAGCAGAATACCCTTATCTTTACCATCTGG                                     >  SRR4302518.2272316/1‑101 (MQ=60)
                                                                  NNNCATGTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGAT                                    <  SRR4302518.7902989/101‑1 (MQ=60)
                                                                  GATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                                    <  SRR4302518.854862/101‑1 (MQ=60)
                                                                   ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                                   <  SRR4302518.285330/101‑1 (MQ=60)
                                                                   AGCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGNNN                                   >  SRR4302518.7747337/1‑101 (MQ=60)
                                                                    TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                                  >  SRR4302518.362632/1‑101 (MQ=60)
                                                                    TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                                  <  SRR4302518.6181389/101‑1 (MQ=60)
                                                                    TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGNNN                                  >  SRR4302518.9463963/1‑101 (MQ=60)
                                                                     TATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                                 <  SRR4302518.4580755/101‑1 (MQ=60)
                                                                     CATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTNNN                                 >  SRR4302518.8131146/1‑101 (MQ=60)
                                                                      NNNTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                                <  SRR4302518.11020041/101‑1 (MQ=60)
                                                                      ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                                <  SRR4302518.1810569/101‑1 (MQ=60)
                                                                      ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGAAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCNNN                                >  SRR4302518.7631932/1‑101 (MQ=60)
                                                                      ATTTGGCAATGGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGgcnnn                                >  SRR4302518.9913360/1‑96 (MQ=60)
                                                                        TTGGCAATTGAAAAACTGGCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                              <  SRR4302518.833972/101‑1 (MQ=60)
                                                                         TGGCAATTGAACATCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                             <  SRR4302518.4838808/101‑1 (MQ=60)
                                                                         NNNCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                             <  SRR4302518.7494737/101‑1 (MQ=60)
                                                                           GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCT                           <  SRR4302518.6652084/101‑1 (MQ=60)
                                                                            CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG                          >  SRR4302518.6568640/1‑101 (MQ=60)
                                                                            CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG                          >  SRR4302518.4080656/1‑101 (MQ=60)
                                                                            CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCACCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG                          >  SRR4302518.5899232/1‑101 (MQ=60)
                                                                              ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGTCGCTGAA                        >  SRR4302518.6824614/1‑101 (MQ=60)
                                                                               TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGACA                       >  SRR4302518.1578396/1‑101 (MQ=60)
                                                                               NNNAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAA                       <  SRR4302518.9536647/101‑1 (MQ=60)
                                                                                TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGANNN                      >  SRR4302518.8707077/1‑101 (MQ=60)
                                                                                TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG                      >  SRR4302518.3954755/1‑101 (MQ=60)
                                                                                 GAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAANNN                     >  SRR4302518.11538809/1‑101 (MQ=60)
                                                                                  NNNAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC                    <  SRR4302518.14888676/101‑1 (MQ=60)
                                                                                  NNNAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC                    <  SRR4302518.11306198/101‑1 (MQ=60)
                                                                                  AAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGATCATGACGCTGAAAGAC                    <  SRR4302518.4243819/101‑1 (MQ=60)
                                                                                  AAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCACTTATCTTTACCATCTGGTAATGACGCTGAcannn                    >  SRR4302518.10452287/1‑96 (MQ=60)
                                                                                   AAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG                   <  SRR4302518.5771459/101‑1 (MQ=60)
                                                                                   AAAACTGACCAGCGCCAGCTTTGCTTTGACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG                   <  SRR4302518.5012062/101‑1 (MQ=60)
                                                                                    GAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCAGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGANNN                  >  SRR4302518.9345519/1‑101 (MQ=60)
                                                                                    AAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCATCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGANNN                  >  SRR4302518.8144640/1‑101 (MQ=60)
                                                                                     AACTTACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCC                 <  SRR4302518.6488271/101‑1 (MQ=60)
                                                                                     AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGGCATGACGCTGAAAGACGCC                 >  SRR4302518.5915333/1‑101 (MQ=60)
                                                                                      NNNGACCAGCGCCAGCTTTGCTTTTACTGTCGATCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA                <  SRR4302518.13588712/101‑1 (MQ=60)
                                                                                      ACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGNNN                >  SRR4302518.7516831/1‑101 (MQ=60)
                                                                                        TGACGAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC              <  SRR4302518.6491783/101‑1 (MQ=60)
                                                                                          ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGG            >  SRR4302518.2255486/1‑101 (MQ=60)
                                                                                           CCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACNNN           >  SRR4302518.11427824/1‑101 (MQ=60)
                                                                                           CCAGCCCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGC           <  SRR4302518.3642718/101‑1 (MQ=60)
                                                                                            CAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGNNN          >  SRR4302518.10504964/1‑101 (MQ=60)
                                                                                            CAGCGCCAGCTGTGCTTTTACTGTCGAACAGCCGCAGCAATGGGCAGCAGAATCCCCTTATCTTTACCATCCGGTCATGACGCTGAAAGACGCCAACGNNN          >  SRR4302518.12853252/1‑101 (MQ=60)
                                                                                              GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAAC        >  SRR4302518.5268178/1‑101 (MQ=60)
                                                                                              GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAAC        >  SRR4302518.3359412/1‑101 (MQ=60)
                                                                                                GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAANNN      >  SRR4302518.9734382/1‑101 (MQ=60)
                                                                                                GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAANNN      >  SRR4302518.7392962/1‑101 (MQ=60)
                                                                                                GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGT      >  SRR4302518.5574547/1‑101 (MQ=60)
                                                                                                GCCAGCTTTGCTTTGACTGTCGAACAGCCGCTGCAATGTTCAGCAGAATCCCCTTATCTTTACGATCTGGTCATGACCCTGAAAGACGCCAACGGCAAaga      >  SRR4302518.1367991/1‑98 (MQ=60)
                                                                                                    NNNTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCCG  <  SRR4302518.11615565/101‑1 (MQ=60)
                                                                                                    |                                                                                                    
CCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTG  >  NC_000913/3220997‑3221197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑