Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,097 2 bp→GC coding (824‑825/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,0970CG100.0% 872.5 / NA 257T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (122/135);  total (122/135)
*NC_0009133,221,0980GC100.0% 872.1 / NA 257T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (122/135);  total (122/135)

Only 100 of 261 total aligned reads displayed.

CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA  >  NC_000913/3221010‑3221184
                                                                                       ||                                                                                      
  cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCt                                                                                  >  1:4068888/1‑93 (MQ=255)
  cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCt                                                                                  <  2:5218927/93‑1 (MQ=255)
   tGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                 >  1:4689660/1‑93 (MQ=255)
   tGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                 >  1:4433769/1‑93 (MQ=255)
   tGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                 >  1:1828008/1‑93 (MQ=255)
   tGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                 >  2:427703/1‑93 (MQ=255)
   tGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                 <  2:2142234/93‑1 (MQ=255)
    ggAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAgctttgcttt                                                                                >  1:146611/1‑93 (MQ=255)
    ggAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAgctttgcttt                                                                                <  2:833889/93‑1 (MQ=255)
     gAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt                                                                               >  1:1042391/1‑93 (MQ=255)
      aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTa                                                                              <  1:749857/93‑1 (MQ=255)
      aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTa                                                                              <  1:2202620/93‑1 (MQ=255)
       atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc                                                                             >  2:1304080/1‑93 (MQ=255)
         ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg                                                                           <  1:1340604/93‑1 (MQ=255)
         ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg                                                                           <  1:5021120/93‑1 (MQ=255)
          taCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                          <  1:6407860/93‑1 (MQ=255)
           aCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTc                                                                         >  2:2818781/1‑93 (MQ=255)
              cTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa                                                                      >  1:2935287/1‑93 (MQ=255)
              cTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa                                                                      <  1:4633531/93‑1 (MQ=255)
               tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                     >  2:4993615/1‑93 (MQ=255)
               tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                     >  2:93162/1‑93 (MQ=255)
               tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                     <  1:5600239/93‑1 (MQ=255)
                gTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa                                                                    <  2:6542592/93‑1 (MQ=255)
                gTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa                                                                    >  2:3591814/1‑93 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  1:6354061/93‑1 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  2:5635834/93‑1 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  1:5480259/93‑1 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  1:2780096/93‑1 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  <  2:702419/93‑1 (MQ=255)
                  ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                  >  1:2970400/1‑93 (MQ=255)
                   tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                 <  1:5135968/93‑1 (MQ=255)
                   tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                 <  1:7171559/93‑1 (MQ=255)
                    gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                >  2:3439124/1‑93 (MQ=255)
                     aTGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                               <  2:4615813/93‑1 (MQ=255)
                      tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgca                                                              >  1:2721701/1‑93 (MQ=255)
                        gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagc                                                            <  2:3011039/93‑1 (MQ=255)
                        gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagc                                                            <  2:5427923/93‑1 (MQ=255)
                           aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                         <  2:3113548/93‑1 (MQ=255)
                              gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                      >  2:3181259/1‑93 (MQ=255)
                               cgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                     <  2:4894974/93‑1 (MQ=255)
                                gtggtgCACAGCAGCGCCATTGATCATTTGGCAATTGGAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                    <  1:6978369/93‑1 (MQ=255)
                                gtggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                    >  1:2292526/1‑93 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   >  1:6817799/1‑93 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   <  1:2546305/93‑1 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   >  1:981800/1‑93 (MQ=255)
                                 tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCACCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                   >  1:1531265/1‑93 (MQ=255)
                                  ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                  >  2:6053116/1‑93 (MQ=255)
                                   gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                 <  2:862407/93‑1 (MQ=255)
                                   gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                 <  2:1575403/93‑1 (MQ=255)
                                    tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagcag                                                >  1:678123/1‑93 (MQ=255)
                                     gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGa                                               >  2:193228/1‑93 (MQ=255)
                                      cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                              <  2:3866601/93‑1 (MQ=255)
                                      cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                              <  1:1936768/93‑1 (MQ=255)
                                      cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                              >  1:3749596/1‑93 (MQ=255)
                                       acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                             >  1:5748537/1‑93 (MQ=255)
                                           cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcccc                                         <  2:3246530/93‑1 (MQ=255)
                                            agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt                                        <  2:1761366/93‑1 (MQ=255)
                                             gcgcCATTGATCATATGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                       >  2:6452849/1‑93 (MQ=255)
                                               gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAt                                     >  2:2351618/1‑93 (MQ=255)
                                                ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc                                    >  1:4233374/1‑93 (MQ=255)
                                                 cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt                                   <  2:6706344/93‑1 (MQ=255)
                                                 cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt                                   >  1:786831/1‑93 (MQ=255)
                                                   ttGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt                                 >  2:4334374/1‑93 (MQ=255)
                                                    tGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTa                                <  1:4362796/93‑1 (MQ=255)
                                                     gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               >  2:3980460/1‑93 (MQ=255)
                                                     gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               >  2:5275769/1‑93 (MQ=255)
                                                     gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               >  1:589702/1‑93 (MQ=255)
                                                     gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                               >  2:3756096/1‑93 (MQ=255)
                                                      aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                              >  2:3800870/1‑93 (MQ=255)
                                                      aTCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                              >  2:7151517/1‑93 (MQ=255)
                                                        cATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                            >  1:1128865/1‑93 (MQ=255)
                                                        cATTTGCCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                            >  2:4720899/1‑93 (MQ=255)
                                                         aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc                           <  2:1582514/93‑1 (MQ=255)
                                                           ttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                         <  2:1036005/93‑1 (MQ=255)
                                                              gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                      >  1:4898733/1‑93 (MQ=255)
                                                              gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                      >  2:2582966/1‑93 (MQ=255)
                                                              gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                      <  1:7094209/93‑1 (MQ=255)
                                                               cAATTGAAAAACTGACCAGCGCTAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                     >  2:2851313/1‑93 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  2:1203157/93‑1 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  1:7315414/93‑1 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  >  1:3707124/1‑93 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  >  1:7037984/1‑93 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  1:418239/93‑1 (MQ=255)
                                                                  ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                  <  2:4399074/93‑1 (MQ=255)
                                                                   tGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAc                 <  1:6595701/93‑1 (MQ=255)
                                                                    gAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACg                >  2:4180470/1‑93 (MQ=255)
                                                                     aaaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGc               >  2:889507/1‑93 (MQ=255)
                                                                     aaaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGc               >  2:5134675/1‑93 (MQ=255)
                                                                       aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTg             >  1:4565731/1‑93 (MQ=255)
                                                                        aaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGa            <  2:1340148/93‑1 (MQ=255)
                                                                         aCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaa           >  1:5979013/1‑93 (MQ=255)
                                                                            gACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGa        <  1:6796362/93‑1 (MQ=255)
                                                                             aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc       <  2:583240/93‑1 (MQ=255)
                                                                               cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc     <  2:3294418/93‑1 (MQ=255)
                                                                               cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc     >  2:5725598/1‑93 (MQ=255)
                                                                               cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc     >  2:240691/1‑93 (MQ=255)
                                                                                aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGcc    <  1:5344386/93‑1 (MQ=255)
                                                                                 gcgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCa   >  1:6087398/1‑93 (MQ=255)
                                                                                  cgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa  <  2:4114983/93‑1 (MQ=255)
                                                                                  cgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa  <  1:3333330/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA  >  NC_000913/3221010‑3221184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  NC_000913/3221004‑3221186
                                                                                             |                                                                                         
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAG                                                                                           <  SRR4302542.7221472/94‑1 (MQ=60)
     ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTG                                                                                      <  SRR4302542.1090652/94‑1 (MQ=60)
      CGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGC                                                                                     <  SRR4302542.4342579/94‑1 (MQ=60)
       GCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCT                                                                                    >  SRR4302542.4109013/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302542.7385773/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302542.4735459/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302542.4477292/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302542.2589604/1‑94 (MQ=60)
        CTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTT                                                                                   >  SRR4302542.1843842/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  <  SRR4302542.7234285/94‑1 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  >  SRR4302542.6841345/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  >  SRR4302542.147841/1‑94 (MQ=60)
         TGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTT                                                                                  <  SRR4302542.2291025/94‑1 (MQ=60)
          GGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTT                                                                                 >  SRR4302542.1051339/1‑94 (MQ=60)
           GAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTA                                                                                <  SRR4302542.5413283/94‑1 (MQ=60)
            AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                               <  SRR4302542.756342/94‑1 (MQ=60)
            AATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAC                                                                               <  SRR4302542.2221581/94‑1 (MQ=60)
              TATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTG                                                                             <  SRR4302542.4650140/94‑1 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            >  SRR4302542.2330517/1‑94 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            >  SRR4302542.2862103/1‑94 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            <  SRR4302542.1352142/94‑1 (MQ=60)
               ATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGT                                                                            <  SRR4302542.5069876/94‑1 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           <  SRR4302542.6471001/94‑1 (MQ=60)
                TACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC                                                                           <  SRR4302542.3071266/94‑1 (MQ=60)
                 ACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCG                                                                          >  SRR4302542.7368553/1‑94 (MQ=60)
                   CCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAA                                                                        >  SRR4302542.2963401/1‑94 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                       <  SRR4302542.3831681/94‑1 (MQ=60)
                    CTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAC                                                                       <  SRR4302542.4678812/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      <  SRR4302542.7597294/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      <  SRR4302542.5654646/94‑1 (MQ=60)
                     TGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACA                                                                      >  SRR4302542.4483214/1‑94 (MQ=60)
                       TTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGC                                                                    >  SRR4302542.2998854/1‑94 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   <  SRR4302542.2806868/94‑1 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   <  SRR4302542.5533488/94‑1 (MQ=60)
                        TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCC                                                                   <  SRR4302542.6416650/94‑1 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  <  SRR4302542.7241905/94‑1 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  <  SRR4302542.4303649/94‑1 (MQ=60)
                         TGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCG                                                                  <  SRR4302542.5185890/94‑1 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                >  SRR4302542.2747939/1‑94 (MQ=60)
                           ATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCA                                                                >  SRR4302542.152318/1‑94 (MQ=60)
                                 AACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATG                                                          >  SRR4302542.2793504/1‑94 (MQ=60)
                                  ACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                         <  SRR4302542.5547108/94‑1 (MQ=60)
                                  ACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGG                                                         <  SRR4302542.1322626/94‑1 (MQ=60)
                                   CGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGT                                                        <  SRR4302542.651537/94‑1 (MQ=60)
                                    GCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTC                                                       <  SRR4302542.4681561/94‑1 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      >  SRR4302542.7418087/1‑94 (MQ=60)
                                     CGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCA                                                      >  SRR4302542.2312219/1‑94 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGGAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                     <  SRR4302542.7046914/94‑1 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                     >  SRR4302542.990254/1‑94 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                     >  SRR4302542.6884820/1‑94 (MQ=60)
                                      GTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCACCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAG                                                     >  SRR4302542.1544487/1‑94 (MQ=60)
                                       TGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGC                                                    <  SRR4302542.2570983/94‑1 (MQ=60)
                                         GTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAG                                                  >  SRR4302542.683979/1‑94 (MQ=60)
                                          TGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGA                                                 >  SRR4302542.1129350/1‑94 (MQ=60)
                                           GCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                >  SRR4302542.3786697/1‑94 (MQ=60)
                                           GCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAA                                                <  SRR4302542.4405042/94‑1 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                               >  SRR4302542.5335280/1‑94 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                               >  SRR4302542.5804334/1‑94 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                               >  SRR4302542.7550821/1‑94 (MQ=60)
                                            CACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAT                                               <  SRR4302542.1953486/94‑1 (MQ=60)
                                             ACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATC                                              <  SRR4302542.2162503/94‑1 (MQ=60)
                                              agGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGTTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCC                                             <  SRR4302542.6288169/92‑1 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                            >  SRR4302542.6867226/1‑94 (MQ=60)
                                               AGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCC                                            <  SRR4302542.1200110/94‑1 (MQ=60)
                                                GCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCC                                           <  SRR4302542.4852428/94‑1 (MQ=60)
                                                 CAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCT                                          >  SRR4302542.3784725/1‑94 (MQ=60)
                                                    CGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                       <  SRR4302542.4427448/94‑1 (MQ=60)
                                                    CGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                       >  SRR4302542.5838194/1‑94 (MQ=60)
                                                    CGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAT                                       <  SRR4302542.5826228/94‑1 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                      >  SRR4302542.4275060/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                      <  SRR4302542.7002201/94‑1 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                      >  SRR4302542.1380748/1‑94 (MQ=60)
                                                     GCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATC                                      >  SRR4302542.1543046/1‑94 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     <  SRR4302542.6898390/94‑1 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     <  SRR4302542.3350827/94‑1 (MQ=60)
                                                      CCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCT                                     >  SRR4302542.793612/1‑94 (MQ=60)
                                                        ATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTT                                   >  SRR4302542.6300437/1‑94 (MQ=60)
                                                        ATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTT                                   <  SRR4302542.3517208/94‑1 (MQ=60)
                                                        ATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTT                                   <  SRR4302542.2834290/94‑1 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 >  SRR4302542.1633644/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 >  SRR4302542.594784/1‑94 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 <  SRR4302542.4938500/94‑1 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 <  SRR4302542.4405634/94‑1 (MQ=60)
                                                          TGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 <  SRR4302542.7139616/94‑1 (MQ=60)
                                                          TGATCATTTGGAAATTGAAAAACAGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAC                                 <  SRR4302542.5669221/94‑1 (MQ=60)
                                                           GATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACC                                <  SRR4302542.51747/94‑1 (MQ=60)
                                                            ATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCA                               >  SRR4302542.4555308/1‑94 (MQ=60)
                                                             TCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAT                              >  SRR4302542.1138499/1‑94 (MQ=60)
                                                              CATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATC                             >  SRR4302542.5043829/1‑94 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            >  SRR4302542.809919/1‑94 (MQ=60)
                                                               ATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCT                            >  SRR4302542.6706156/1‑94 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                         <  SRR4302542.423948/94‑1 (MQ=60)
                                                                  TGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGT                         <  SRR4302542.6879287/94‑1 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                        <  SRR4302542.5559813/94‑1 (MQ=60)
                                                                   GGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTC                        >  SRR4302542.4946351/1‑94 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                       <  SRR4302542.7163808/94‑1 (MQ=60)
                                                                    GCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCA                       >  SRR4302542.6860034/1‑94 (MQ=60)
                                                                     CAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAT                      >  SRR4302542.2124298/1‑94 (MQ=60)
                                                                      AATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG                     >  SRR4302542.657557/1‑94 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    >  SRR4302542.4272491/1‑94 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    >  SRR4302542.3743806/1‑94 (MQ=60)
                                                                       ATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGA                    >  SRR4302542.7107111/1‑94 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                   <  SRR4302542.7387071/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                   <  SRR4302542.2997907/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                   <  SRR4302542.6031090/94‑1 (MQ=60)
                                                                        TTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGAC                   <  SRR4302542.421840/94‑1 (MQ=60)
                                                                         TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                  <  SRR4302542.6660688/94‑1 (MQ=60)
                                                                         TGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACG                  <  SRR4302542.6144083/94‑1 (MQ=60)
                                                                            AAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG               >  SRR4302542.4610429/1‑94 (MQ=60)
                                                                             AAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCTTCTGGTCATGACGCTGA              >  SRR4302542.1000810/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAA             >  SRR4302542.6036885/1‑94 (MQ=60)
                                                                              AACTGACCAGCGCCAGCTTTGCTTTGACTGTCGAACAGCTGCAGCAATGGTCAGCAGAATCCCCTTAGCTTTACCATCTGTTCATGACGCTGAA             <  SRR4302542.3560050/94‑1 (MQ=60)
                                                                                CTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAG           <  SRR4302542.5089458/94‑1 (MQ=60)
                                                                                 TGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGA          <  SRR4302542.4640000/94‑1 (MQ=60)
                                                                                 TGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGA          >  SRR4302542.5355867/1‑94 (MQ=60)
                                                                                 TGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGA          >  SRR4302542.6463777/1‑94 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         <  SRR4302542.6021964/94‑1 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         <  SRR4302542.6863193/94‑1 (MQ=60)
                                                                                  GACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAC         <  SRR4302542.2504488/94‑1 (MQ=60)
                                                                                   GCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        <  SRR4302542.979609/94‑1 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        >  SRR4302542.4170877/1‑94 (MQ=60)
                                                                                   ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACG        >  SRR4302542.7148020/1‑94 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA     <  SRR4302542.7065386/94‑1 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA     >  SRR4302542.6146339/1‑94 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA     <  SRR4302542.5396303/94‑1 (MQ=60)
                                                                                      AGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCA     <  SRR4302542.2736371/94‑1 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA    <  SRR4302542.408382/94‑1 (MQ=60)
                                                                                       GCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAA    >  SRR4302542.1185032/1‑94 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   <  SRR4302542.1800407/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   <  SRR4302542.1892940/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   <  SRR4302542.3364697/94‑1 (MQ=60)
                                                                                        CGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC   >  SRR4302542.5229320/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  SRR4302542.2719661/1‑94 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  <  SRR4302542.620891/94‑1 (MQ=60)
                                                                                         GCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  <  SRR4302542.5735331/94‑1 (MQ=60)
                                                                                             |                                                                                         
TCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACG  >  NC_000913/3221004‑3221186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑