Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,221,392	T→C	R373R (CGT→CGC)	b3076 →	evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,221,392	0	T	C	84.8%	633.0 / 107.5	283	R373R (CGT→CGC)	b3076	evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand): ref base T (24/19); new base C (115/125); total (139/144)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 285 total aligned reads displayed.

CAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCAC  >  NC_000913/3221302‑3221482

   cGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGc                                                                                       <  1:973038/93‑1 (MQ=255)

    gATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCt                                                                                      >  1:4819815/1‑93 (MQ=255)

      tcatcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCa                                                                                    >  2:4154377/1‑93 (MQ=255)

        atcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACt                                                                                  <  2:5304335/93‑1 (MQ=255)

         tcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTa                                                                                 >  2:3456151/1‑93 (MQ=255)

           gCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTAcc                                                                               >  1:772320/1‑93 (MQ=255)

           gCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTAcc                                                                               <  2:2643844/93‑1 (MQ=255)

            cAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTAccc                                                                              <  2:6800361/93‑1 (MQ=255)

             aaaGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCg                                                                             <  1:1274367/93‑1 (MQ=255)

               aGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGaa                                                                           <  1:71766/93‑1 (MQ=255)

               aGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGaa                                                                           >  1:2235844/1‑93 (MQ=255)

               aGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGaa                                                                           <  2:2233672/93‑1 (MQ=255)

               aGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGaa                                                                           <  1:3082697/93‑1 (MQ=255)

                ggCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAAc                                                                          <  1:2708746/93‑1 (MQ=255)

                ggCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAAc                                                                          <  1:101754/93‑1 (MQ=255)

                 gCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACg                                                                         >  2:6993302/1‑93 (MQ=255)

                     cgcCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATcc                                                                     <  1:2874038/93‑1 (MQ=255)

                     cgcCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATcc                                                                     <  2:4641784/93‑1 (MQ=255)

                     cgcCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATcc                                                                     >  2:663514/1‑93 (MQ=255)

                       ccGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCcgc                                                                   >  1:3738685/1‑93 (MQ=255)

                        cGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCcgcg                                                                  >  2:278537/1‑93 (MQ=255)

                         gTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGt                                                                 >  1:3188486/1‑93 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGtt                                                                >  1:4507393/1‑93 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                >  2:6539879/1‑93 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                <  2:5178996/93‑1 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                <  2:4722787/93‑1 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                >  1:113419/1‑93 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                >  1:1638057/1‑93 (MQ=255)

                          ttGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtt                                                                >  1:1887528/1‑93 (MQ=255)

                            ggAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGtttt                                                              <  1:427703/93‑1 (MQ=255)

                              aaTGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTa                                                            <  2:2939984/93‑1 (MQ=255)

                               aTGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAc                                                           >  2:1456201/1‑93 (MQ=255)

                               aTGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAc                                                           >  2:4226202/1‑93 (MQ=255)

                                tGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACg                                                          >  2:7260390/1‑93 (MQ=255)

                                 ggATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGa                                                         >  2:6577188/1‑93 (MQ=255)

                                  gATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGaa                                                        <  1:3922223/93‑1 (MQ=255)

                                   aTCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAc                                                       >  2:5072934/1‑93 (MQ=255)

                                   aTCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAc                                                       >  2:213585/1‑93 (MQ=255)

                                    tCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                      >  1:5012908/1‑93 (MQ=255)

                                    tCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                      >  1:4097563/1‑93 (MQ=255)

                                    tCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                      >  1:5854127/1‑93 (MQ=255)

                                    tCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                      <  1:5942181/93‑1 (MQ=255)

                                     cgcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtg                                                     <  2:151035/93‑1 (MQ=255)

                                     cgcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtg                                                     <  2:3745427/93‑1 (MQ=255)

                                     cgcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtg                                                     <  1:7247913/93‑1 (MQ=255)

                                      gcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgt                                                    >  2:6079601/1‑93 (MQ=255)

                                      gcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgt                                                    >  2:4561337/1‑93 (MQ=255)

                                       cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtg                                                   <  1:6834899/93‑1 (MQ=255)

                                       cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtg                                                   <  1:6171283/93‑1 (MQ=255)

                                        gTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt                                                  >  2:4041031/1‑93 (MQ=255)

                                        gTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt                                                  <  2:786831/93‑1 (MQ=255)

                                          cGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGa                                                <  2:6003753/93‑1 (MQ=255)

                                           gagaAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGat                                               >  1:1744167/1‑93 (MQ=255)

                                            agaAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGata                                              <  2:2825918/93‑1 (MQ=255)

                                             gaAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGatat                                             >  1:3357010/1‑93 (MQ=255)

                                             gaAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGatat                                             >  1:281365/1‑93 (MQ=255)

                                              aaaGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATc                                            <  2:3301257/93‑1 (MQ=255)

                                               aaGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCt                                           >  1:5456846/1‑93 (MQ=255)

                                                aGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTa                                          <  2:2341280/93‑1 (MQ=255)

                                                aGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTa                                          >  1:2469379/1‑93 (MQ=255)

                                                 gATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTAc                                         >  2:4322710/1‑93 (MQ=255)

                                                 gATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTAc                                         >  2:4424019/1‑93 (MQ=255)

                                                  aTCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACg                                        <  1:2758902/93‑1 (MQ=255)

                                                   tctcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACgg                                       <  2:1600229/93‑1 (MQ=255)

                                                   tctcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACgg                                       >  1:542368/1‑93 (MQ=255)

                                                     tcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGcc                                     >  2:6814032/1‑93 (MQ=255)

                                                      ccAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCt                                    >  2:180783/1‑93 (MQ=255)

                                                       cAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTg                                   >  2:3068676/1‑93 (MQ=255)

                                                        aGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGt                                  >  1:3481710/1‑93 (MQ=255)

                                                          ttGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGttt                                >  1:26233/1‑93 (MQ=255)

                                                          ttGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGttt                                <  1:2992360/93‑1 (MQ=255)

                                                          ttGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGttt                                >  2:3547830/1‑93 (MQ=255)

                                                          ttGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGttt                                <  1:4180470/93‑1 (MQ=255)

                                                          ttGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCATGctt                                >  1:6969452/1‑93 (MQ=255)

                                                           tgatgaAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTtg                               <  1:5069585/93‑1 (MQ=255)

                                                           tgatgaAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTtg                               <  1:3649966/93‑1 (MQ=255)

                                                            gatgaAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTtgt                              <  2:5880972/93‑1 (MQ=255)

                                                              tgaAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGa                            >  2:7137357/1‑93 (MQ=255)

                                                               gaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAt                           <  1:3591814/93‑1 (MQ=255)

                                                               gaAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAt                           <  1:6660913/93‑1 (MQ=255)

                                                                aaGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATg                          >  2:1280344/1‑93 (MQ=255)

                                                                 agcagcACAATATCAACTCCGTGCGTCCCGCTCACTACCCGAGCGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATgg                         <  1:5339234/93‑1 (MQ=255)

                                                                 agcagcACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATgg                         <  2:2693684/93‑1 (MQ=255)

                                                                 agcagcACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATgg                         <  2:615550/93‑1 (MQ=255)

                                                                  gcagcaCAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGc                        <  1:4973312/93‑1 (MQ=255)

                                                                      cacaATATCAACTCCGTGCGCACCGCTAACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGaa                    >  2:6430220/1‑93 (MQ=255)

                                                                         aaTATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAAcc                 >  1:6294486/1‑93 (MQ=255)

                                                                           tatCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGa               <  2:3379499/93‑1 (MQ=255)

                                                                            atCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGAc              <  1:846383/93‑1 (MQ=255)

                                                                             tCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACg             >  1:1512061/1‑93 (MQ=255)

                                                                             tCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACg             <  1:5689398/93‑1 (MQ=255)

                                                                              cAACTCCGTGCGCGCCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGGGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGt            <  1:4756583/93‑1 (MQ=255)

                                                                              cAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGt            <  2:3676296/93‑1 (MQ=255)

                                                                               aaCTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTc           <  1:4637817/93‑1 (MQ=255)

                                                                                  tCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGaa        >  1:4341675/1‑93 (MQ=255)

                                                                                   ccGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAAt       >  2:5751611/1‑93 (MQ=255)

                                                                                   ccGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAAt       <  2:6784819/93‑1 (MQ=255)

                                                                                      tGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGc    >  2:855828/1‑93 (MQ=255)

                                                                                      tGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAAGAACTGTGAGATATCCACGGCCTGTTTGTCATGGCGGTAACCCGCGGCGAATCGc    >  1:2043122/1‑93 (MQ=255)

                                                                                       gcgcACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCa   >  1:5762750/1‑93 (MQ=255)

CAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCAC  >  NC_000913/3221302‑3221482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGC  >  NC_000913/3221299‑3221485

CGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGC                                                                                               <  SRR4302542.3018396/94‑1 (MQ=60)

 GACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCA                                                                                              >  SRR4302542.5997081/1‑94 (MQ=60)

 GACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCA                                                                                              >  SRR4302542.3431326/1‑94 (MQ=60)

  ACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCAC                                                                                             <  SRR4302542.1080824/94‑1 (MQ=60)

  ACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCAC                                                                                             >  SRR4302542.439102/1‑94 (MQ=60)

  ACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCAC                                                                                             <  SRR4302542.242752/94‑1 (MQ=60)

   CAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACC                                                                                            <  SRR4302542.284508/94‑1 (MQ=60)

    AACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCG                                                                                           >  SRR4302542.5009305/1‑94 (MQ=60)

      CGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCT                                                                                         <  SRR4302542.981426/94‑1 (MQ=60)

      CGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCT                                                                                         >  SRR4302542.4866789/1‑94 (MQ=60)

         TCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCAC                                                                                      <  SRR4302542.5042126/94‑1 (MQ=60)

          CATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACT                                                                                     <  SRR4302542.4141514/94‑1 (MQ=60)

          CATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACT                                                                                     >  SRR4302542.610696/1‑94 (MQ=60)

          CATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACT                                                                                     >  SRR4302542.5505492/1‑94 (MQ=60)

            TCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCCCTAC                                                                                   <  SRR4302542.7035045/94‑1 (MQ=60)

            TCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTAC                                                                                   >  SRR4302542.3888409/1‑94 (MQ=60)

             CGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACC                                                                                  >  SRR4302542.5975012/1‑94 (MQ=60)

             CGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACC                                                                                  >  SRR4302542.778995/1‑94 (MQ=60)

              GCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCC                                                                                 <  SRR4302542.1098243/94‑1 (MQ=60)

               CAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGATGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCG                                                                                <  SRR4302542.6274240/94‑1 (MQ=60)

               CAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCG                                                                                >  SRR4302542.483594/1‑94 (MQ=60)

                AAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGA                                                                               >  SRR4302542.4591445/1‑94 (MQ=60)

                AAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGA                                                                               <  SRR4302542.1285311/94‑1 (MQ=60)

                 AAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAA                                                                              >  SRR4302542.1154140/1‑94 (MQ=60)

                 AAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAA                                                                              >  SRR4302542.2255074/1‑94 (MQ=60)

                  AGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAAC                                                                             <  SRR4302542.4766918/94‑1 (MQ=60)

                  AGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAAC                                                                             <  SRR4302542.72360/94‑1 (MQ=60)

                  AGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAAC                                                                             <  SRR4302542.2829751/94‑1 (MQ=60)

                  AGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAAC                                                                             <  SRR4302542.3112046/94‑1 (MQ=60)

                   GGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACG                                                                            <  SRR4302542.102604/94‑1 (MQ=60)

                   GGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACG                                                                            <  SRR4302542.2734874/94‑1 (MQ=60)

                      CGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATC                                                                         >  SRR4302542.5799571/1‑94 (MQ=60)

                      CGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATC                                                                         >  SRR4302542.5815856/1‑94 (MQ=60)

                      CGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATC                                                                         >  SRR4302542.6891918/1‑94 (MQ=60)

                        CGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCG                                                                       <  SRR4302542.2901635/94‑1 (MQ=60)

                         GCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGC                                                                      >  SRR4302542.3775692/1‑94 (MQ=60)

                          CCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCG                                                                     >  SRR4302542.6717559/1‑94 (MQ=60)

                           CGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGT                                                                    >  SRR4302542.3218688/1‑94 (MQ=60)

                            GTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTT                                                                   >  SRR4302542.4551570/1‑94 (MQ=60)

                            GTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTT                                                                   >  SRR4302542.1903868/1‑94 (MQ=60)

                            GTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTT                                                                   >  SRR4302542.114350/1‑94 (MQ=60)

                            GTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTT                                                                   >  SRR4302542.1652216/1‑94 (MQ=60)

                             TTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTT                                                                  >  SRR4302542.1674011/1‑94 (MQ=60)

                              TGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTT                                                                 <  SRR4302542.5327037/94‑1 (MQ=60)

                               GGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTT                                                                <  SRR4302542.207470/94‑1 (MQ=60)

                               GGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTT                                                                <  SRR4302542.431401/94‑1 (MQ=60)

                                GAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTA                                                               >  SRR4302542.3137054/1‑94 (MQ=60)

                                 AATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAC                                                              <  SRR4302542.160443/94‑1 (MQ=60)

                                 AATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAC                                                              <  SRR4302542.5904124/94‑1 (MQ=60)

                                  ATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACG                                                             >  SRR4302542.2062109/1‑94 (MQ=60)

                                  ATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACG                                                             <  SRR4302542.386189/94‑1 (MQ=60)

                                   TGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGA                                                            >  SRR4302542.2977222/1‑94 (MQ=60)

                                     GATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAC                                                          >  SRR4302542.4109747/1‑94 (MQ=60)

                                     GATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAC                                                          <  SRR4302542.3960926/94‑1 (MQ=60)

                                      ATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACT                                                         >  SRR4302542.4287821/1‑94 (MQ=60)

                                      ATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACT                                                         >  SRR4302542.4137979/1‑94 (MQ=60)

                                      ATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACT                                                         >  SRR4302542.5910903/1‑94 (MQ=60)

                                      ATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACT                                                         >  SRR4302542.5061592/1‑94 (MQ=60)

                                       TCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTGCGAACTG                                                        >  SRR4302542.1476173/1‑94 (MQ=60)

                                       TCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTG                                                        <  SRR4302542.5999746/94‑1 (MQ=60)

                                       TCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTG                                                        <  SRR4302542.427853/94‑1 (MQ=60)

                                        CGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGT                                                       >  SRR4302542.5165568/1‑94 (MQ=60)

                                        CGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGT                                                       <  SRR4302542.7318980/94‑1 (MQ=60)

                                         GCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTG                                                      <  SRR4302542.3471318/94‑1 (MQ=60)

                                          CGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGT                                                     <  SRR4302542.6902059/94‑1 (MQ=60)

                                          CGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGT                                                     <  SRR4302542.6231090/94‑1 (MQ=60)

                                           GTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTG                                                    <  SRR4302542.4019728/94‑1 (MQ=60)

                                           GTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTG                                                    <  SRR4302542.3087349/94‑1 (MQ=60)

                                             CGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGAT                                                  >  SRR4302542.1759300/1‑94 (MQ=60)

                                             CGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGAT                                                  >  SRR4302542.7276243/1‑94 (MQ=60)

                                               AGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATAT                                                >  SRR4302542.3388576/1‑94 (MQ=60)

                                               AGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATAT                                                >  SRR4302542.1458439/1‑94 (MQ=60)

                                               AGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATAT                                                >  SRR4302542.283811/1‑94 (MQ=60)

                                                GAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATC                                               <  SRR4302542.4327136/94‑1 (MQ=60)

                                                GAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATC                                               >  SRR4302542.1881013/1‑94 (MQ=60)

                                                 AAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCT                                              >  SRR4302542.5509854/1‑94 (MQ=60)

                                                 AAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCT                                              <  SRR4302542.6737908/94‑1 (MQ=60)

                                                 AAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCT                                              <  SRR4302542.4633795/94‑1 (MQ=60)

                                                 AAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCT                                              >  SRR4302542.808078/1‑94 (MQ=60)

                                                  AAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTA                                             >  SRR4302542.2493349/1‑94 (MQ=60)

                                                   AGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTAC                                            >  SRR4302542.1872038/1‑94 (MQ=60)

                                                   AGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTAC                                            <  SRR4302542.887476/94‑1 (MQ=60)

                                                    GATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACG                                           <  SRR4302542.226748/94‑1 (MQ=60)

                                                    GATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACG                                           <  SRR4302542.3439206/94‑1 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          <  SRR4302542.2785486/94‑1 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.6124227/1‑94 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.7066735/1‑94 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.6288448/1‑94 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.547056/1‑94 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.495733/1‑94 (MQ=60)

                                                     ATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGG                                          >  SRR4302542.1249489/1‑94 (MQ=60)

                                                        TCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCT                                       >  SRR4302542.3324968/1‑94 (MQ=60)

                                                         CCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTG                                      <  SRR4302542.805610/94‑1 (MQ=60)

                                                         CCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTG                                      <  SRR4302542.2371811/94‑1 (MQ=60)

                                                          CAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGT                                     >  SRR4302542.3514288/1‑94 (MQ=60)

                                                          CAGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGT                                     <  SRR4302542.2909930/94‑1 (MQ=60)

                                                           AGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTT                                    >  SRR4302542.6665573/1‑94 (MQ=60)

                                                           AGTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTT                                    >  SRR4302542.2510253/1‑94 (MQ=60)

                                                            GTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTT                                   >  SRR4302542.26461/1‑94 (MQ=60)

                                                            GTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTT                                   <  SRR4302542.356430/94‑1 (MQ=60)

                                                            GTTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCATGCTT                                   >  SRR4302542.7037915/1‑94 (MQ=60)

                                                             TTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTG                                  <  SRR4302542.3020997/94‑1 (MQ=60)

                                                             TTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTG                                  <  SRR4302542.4172564/94‑1 (MQ=60)

                                                             TTGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTG                                  <  SRR4302542.4221695/94‑1 (MQ=60)

                                                              TGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGT                                 <  SRR4302542.5118848/94‑1 (MQ=60)

                                                              TGATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGT                                 <  SRR4302542.3684056/94‑1 (MQ=60)

                                                               GATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTG                                <  SRR4302542.4454775/94‑1 (MQ=60)

                                                               GATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTG                                >  SRR4302542.7332568/1‑94 (MQ=60)

                                                                ATGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGA                               <  SRR4302542.5597793/94‑1 (MQ=60)

                                                                 TGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAT                              <  SRR4302542.2590716/94‑1 (MQ=60)

                                                                 TGAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAT                              <  SRR4302542.1551684/94‑1 (MQ=60)

                                                                  GAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATG                             <  SRR4302542.3625313/94‑1 (MQ=60)

                                                                  GAAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATG                             <  SRR4302542.6726540/94‑1 (MQ=60)

                                                                   AAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGG                            >  SRR4302542.2657180/1‑94 (MQ=60)

                                                                   AAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGG                            >  SRR4302542.1741225/1‑94 (MQ=60)

                                                                   AAGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGG                            <  SRR4302542.5463869/94‑1 (MQ=60)

                                                                    AGCAGCACAATATCAACTCCGTGCGTCCCGCTCACTACCCGAGCGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGC                           <  SRR4302542.5391109/94‑1 (MQ=60)

                                                                    AGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGC                           <  SRR4302542.939067/94‑1 (MQ=60)

                                                                    AGCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGC                           <  SRR4302542.3583220/94‑1 (MQ=60)

                                                                     GCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCG                          <  SRR4302542.3138527/94‑1 (MQ=60)

                                                                     GCAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCG                          <  SRR4302542.5021594/94‑1 (MQ=60)

                                                                      CAGCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGG                         <  SRR4302542.5092145/94‑1 (MQ=60)

                                                                        GCACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAA                       >  SRR4302542.3332796/1‑94 (MQ=60)

                                                                          ACAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAAC                     <  SRR4302542.276641/94‑1 (MQ=60)

                                                                           CAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACC                    <  SRR4302542.5489343/94‑1 (MQ=60)

                                                                           CAATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACC                    >  SRR4302542.6356502/1‑94 (MQ=60)

                                                                             ATATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCC‑GTTTGTGATGGCGGAAACCGAC                 <  SRR4302542.6999098/94‑1 (MQ=60)

                                                                              TATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGAC                 >  SRR4302542.193574/1‑94 (MQ=60)

                                                                               ATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACG                >  SRR4302542.1525122/1‑94 (MQ=60)

                                                                               ATCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACG                <  SRR4302542.853677/94‑1 (MQ=60)

                                                                                TCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGT               <  SRR4302542.5744649/94‑1 (MQ=60)

                                                                                TCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGT               >  SRR4302542.3716454/1‑94 (MQ=60)

                                                                                TCAACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGT               <  SRR4302542.7255171/94‑1 (MQ=60)

                                                                                 CAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTC              >  SRR4302542.2677354/1‑94 (MQ=60)

                                                                                 CAACTCCGTGCGCGCCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGGGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTC              <  SRR4302542.4802974/94‑1 (MQ=60)

                                                                                  AACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCG             >  SRR4302542.2790884/1‑94 (MQ=60)

                                                                                  AACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCG             <  SRR4302542.5675009/94‑1 (MQ=60)

                                                                                  AACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCG             >  SRR4302542.3769419/1‑94 (MQ=60)

                                                                                  AACTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCG             <  SRR4302542.4683132/94‑1 (MQ=60)

                                                                                    CTCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAA           >  SRR4302542.4384332/1‑94 (MQ=60)

                                                                                     TCCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAAT          <  SRR4302542.5046018/94‑1 (MQ=60)

                                                                                      CCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATC         >  SRR4302542.5835592/1‑94 (MQ=60)

                                                                                      CCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATC         <  SRR4302542.474066/94‑1 (MQ=60)

                                                                                      CCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATC         >  SRR4302542.2775554/1‑94 (MQ=60)

                                                                                      CCGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATC         <  SRR4302542.5427362/94‑1 (MQ=60)

                                                                                       CGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCG        >  SRR4302542.3633004/1‑94 (MQ=60)

                                                                                       CGTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCG        <  SRR4302542.4853090/94‑1 (MQ=60)

                                                                                        GTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGC       >  SRR4302542.3084738/1‑94 (MQ=60)

                                                                                        GTGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTAAGAACTGTGAGATATCCACGGCCTGTTTGTCATGGCGGTAACCCGCGGCGAATCGC       >  SRR4302542.2060787/1‑94 (MQ=57)

                                                                                         TGCGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCA      >  SRR4302542.5818683/1‑94 (MQ=60)

                                                                                          GCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCAC     >  SRR4302542.7455093/1‑94 (MQ=60)

                                                                                           CGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGGTGGCGGCAACAGGCGGCGAATCGCGCG    >  SRR4302542.3877956/1‑94 (MQ=60)

                                                                                           CGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACG    >  SRR4302542.5281781/1‑94 (MQ=60)

                                                                                           CGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACG    <  SRR4302542.6854019/94‑1 (MQ=60)

                                                                                           CGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACG    <  SRR4302542.2625462/94‑1 (MQ=60)

                                                                                           CGCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACG    <  SRR4302542.6854020/94‑1 (MQ=60)

                                                                                            GCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGG   <  SRR4302542.4370103/94‑1 (MQ=60)

                                                                                            GCACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGG   <  SRR4302542.2746652/94‑1 (MQ=60)

                                                                                             CACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGC  >  SRR4302542.1130990/1‑94 (MQ=60)

                                                                                             CACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGC  >  SRR4302542.5949903/1‑94 (MQ=60)

CGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGC  >  NC_000913/3221299‑3221485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`