Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,236,758 Δ1 bp coding (537/966 nt) ygjT → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,7570T.99.3% 1339.1 / ‑3.8 293coding (536/966 nt)ygjTputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (132/159);  total (132/161)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT  >  NC_000913/3236666‑3236844
                                                                                            |                                                                                       
gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGt                                                                                         >  1:6186633/1‑93 (MQ=255)
 ccTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtc                                                                                        >  1:790536/1‑92 (MQ=255)
  cTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtca                                                                                       <  2:5092321/93‑3 (MQ=255)
   ttCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtcat                                                                                      >  1:323622/1‑90 (MQ=255)
     ccTGCTGTTTCCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCAttt                                                                                   <  2:4573869/93‑1 (MQ=255)
     ccTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCAttt                                                                                   <  1:4933590/93‑1 (MQ=255)
      ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTg                                                                                  <  2:5191260/93‑1 (MQ=255)
      ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTg                                                                                  >  2:2896004/1‑93 (MQ=255)
      ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTg                                                                                  <  1:6010086/93‑1 (MQ=255)
       tgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                 >  1:4560223/1‑93 (MQ=255)
       tgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                 >  2:1669884/1‑93 (MQ=255)
            tttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATg                                                                            >  2:5468986/1‑93 (MQ=255)
             ttcccGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGa                                                                           <  1:7235860/90‑1 (MQ=255)
             ttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGa                                                                           <  2:275518/93‑1 (MQ=255)
             ttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGa                                                                           >  1:6989227/1‑93 (MQ=255)
             ttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGa                                                                           <  1:4371889/93‑1 (MQ=255)
              tACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAc                                                                          <  2:332651/93‑1 (MQ=255)
              tACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAc                                                                          <  2:6747214/93‑1 (MQ=255)
               aCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                         <  1:539873/93‑1 (MQ=255)
                 cGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGa                                                                       <  2:3865860/93‑1 (MQ=255)
                   gCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGaca                                                                     >  1:1218991/1‑93 (MQ=255)
                      tGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCa                                                                  >  1:306433/1‑93 (MQ=255)
                      tGAAGATGGCGCCCCCCAATGAAGATAAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCa                                                                  <  1:6277001/93‑1 (MQ=255)
                       gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAt                                                                 >  2:1599827/1‑93 (MQ=255)
                       gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAt                                                                 <  2:162612/93‑1 (MQ=255)
                       gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAt                                                                 <  2:1754303/93‑1 (MQ=255)
                        aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                >  2:5032813/1‑93 (MQ=255)
                        aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                <  1:5047397/93‑1 (MQ=255)
                        aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                <  2:5763755/93‑1 (MQ=255)
                         aGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCg                                                               >  1:4038037/1‑93 (MQ=255)
                          gATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGa                                                              >  2:3302463/1‑93 (MQ=255)
                          gATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGa                                                              <  2:644121/93‑1 (MQ=255)
                             ggCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACaa                                                           <  2:6262964/93‑1 (MQ=255)
                              gcgcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                          >  1:3450619/1‑93 (MQ=255)
                              gcccTCGCCCATGAAGATGAATCAGGCATTGGCGAAAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                          <  1:1285144/90‑1 (MQ=255)
                                gcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                        <  2:5822601/93‑1 (MQ=255)
                                gcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                        <  2:395854/93‑1 (MQ=255)
                                gcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                        >  1:2073123/1‑93 (MQ=255)
                                gcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                        >  1:4320525/1‑93 (MQ=255)
                                 cTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAg                                                       <  2:6567830/93‑1 (MQ=255)
                                 cTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAg                                                       >  1:4640852/1‑93 (MQ=255)
                                  tCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGc                                                      <  2:5323301/93‑1 (MQ=255)
                                  tCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGc                                                      <  1:3427151/93‑1 (MQ=255)
                                   cGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCa                                                     <  2:938112/93‑1 (MQ=255)
                                     cccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAtt                                                   <  2:2671872/93‑1 (MQ=255)
                                     cccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAtt                                                   >  2:338356/1‑93 (MQ=255)
                                     cccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAtt                                                   <  2:817678/93‑1 (MQ=255)
                                     cccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAtt                                                   <  2:7241801/93‑1 (MQ=255)
                                     cccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAtt                                                   >  1:4861402/1‑93 (MQ=255)
                                      ccATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                  >  2:1175820/1‑93 (MQ=255)
                                       cATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAGACCATCGACAACGAGCATTTc                                                 >  2:4433277/1‑93 (MQ=255)
                                        aTGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCt                                                <  2:2417577/93‑1 (MQ=255)
                                        aTGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCt                                                >  1:5290195/1‑93 (MQ=255)
                                         tGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCtt                                               >  1:245989/1‑93 (MQ=255)
                                          gAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCttt                                              >  1:1041211/1‑93 (MQ=255)
                                              aTGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGc                                          >  1:2987802/1‑93 (MQ=255)
                                               tGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCg                                         >  1:2179884/1‑93 (MQ=255)
                                                gAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGt                                        >  2:1254922/1‑93 (MQ=255)
                                                 aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTa                                       >  2:1322121/1‑93 (MQ=255)
                                                  aTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTaa                                      >  2:1403309/1‑93 (MQ=255)
                                                   tCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAg                                     >  1:980650/1‑93 (MQ=255)
                                                   tCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAg                                     >  1:807325/1‑93 (MQ=255)
                                                   tCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAg                                     <  2:1639871/93‑1 (MQ=255)
                                                     aGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGaa                                   <  2:4709500/93‑1 (MQ=255)
                                                      ggCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAt                                  >  1:3566628/1‑93 (MQ=255)
                                                        cATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATgg                                >  2:2137381/1‑93 (MQ=255)
                                                         aTTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGa                               >  1:6608943/1‑93 (MQ=255)
                                                          ttGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                              >  1:900930/1‑93 (MQ=255)
                                                          ttGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                              <  1:1557967/93‑1 (MQ=255)
                                                          ttGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                              >  2:6663933/1‑93 (MQ=255)
                                                           tGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAtt                             <  2:1912861/93‑1 (MQ=255)
                                                             gCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGc                           <  1:6546856/93‑1 (MQ=255)
                                                               gACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTg                         >  2:447207/1‑93 (MQ=255)
                                                                  aagccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAc                      >  2:1785722/5‑93 (MQ=255)
                                                                  aagccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAc                      >  2:4508468/5‑93 (MQ=255)
                                                                   agccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                     >  2:177015/4‑93 (MQ=255)
                                                                   agccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                     >  2:2050513/4‑93 (MQ=255)
                                                                   agccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                     >  1:1637261/4‑93 (MQ=255)
                                                                    gccgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGc                    <  1:3147983/91‑1 (MQ=255)
                                                                       gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCcac                 >  1:376148/1‑93 (MQ=255)
                                                                       gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCcac                 <  1:1695423/93‑1 (MQ=255)
                                                                        cTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCcaca                >  1:5864585/1‑93 (MQ=255)
                                                                         tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac               <  2:3588979/93‑1 (MQ=255)
                                                                         tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac               >  2:2285557/1‑93 (MQ=255)
                                                                         tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac               >  2:2570411/1‑93 (MQ=255)
                                                                          ggTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACAcc              >  2:5936146/1‑93 (MQ=255)
                                                                            tGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc            <  2:3015374/93‑1 (MQ=255)
                                                                            tGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc            <  1:3684098/93‑1 (MQ=255)
                                                                            tGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc            <  1:5755218/93‑1 (MQ=255)
                                                                            tGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc            <  2:1526468/93‑1 (MQ=255)
                                                                             gcgcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCt           >  2:6049599/1‑93 (MQ=255)
                                                                               gcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGa         <  1:6483770/93‑1 (MQ=255)
                                                                                cTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAt        >  2:5657434/1‑93 (MQ=255)
                                                                                cTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAt        <  2:368810/93‑1 (MQ=255)
                                                                                  ggCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGc      <  1:7097608/93‑1 (MQ=255)
                                                                                  ggCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGc      >  2:7018715/1‑93 (MQ=255)
                                                                                     tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTgg   >  1:6706860/1‑93 (MQ=255)
                                                                                      aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt  >  1:2557132/1‑93 (MQ=255)
                                                                                      aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt  >  1:6761955/1‑93 (MQ=255)
                                                                                      aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAACATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt  >  2:7385628/1‑93 (MQ=255)
                                                                                            |                                                                                       
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT  >  NC_000913/3236666‑3236844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  NC_000913/3236666‑3236851
                                                                                             |                                                                                             
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGTC                                                                                               >  SRR4302542.3375383/1‑94 (MQ=60)
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGTC                                                                                               >  SRR4302542.797347/1‑94 (MQ=60)
  CTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCAT                                                                                            >  SRR4302542.326432/1‑94 (MQ=60)
  CTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCAT                                                                                            <  SRR4302542.2357230/94‑1 (MQ=60)
   TTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATT                                                                                           >  SRR4302542.834529/1‑94 (MQ=60)
   TTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATT                                                                                           >  SRR4302542.41906/1‑94 (MQ=60)
    TCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                          <  SRR4302542.3867131/94‑1 (MQ=60)
    TCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                          <  SRR4302542.2597768/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         <  SRR4302542.310575/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         <  SRR4302542.5119196/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         <  SRR4302542.4981547/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCACTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         <  SRR4302542.6269108/94‑1 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        <  SRR4302542.6068284/94‑1 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        >  SRR4302542.4604879/1‑94 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        >  SRR4302542.3469671/1‑94 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        >  SRR4302542.5997029/1‑94 (MQ=60)
       TGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCG                                                                                       <  SRR4302542.5915307/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    >  SRR4302542.6263107/1‑94 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    >  SRR4302542.6707481/1‑94 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302542.351190/94‑1 (MQ=60)
            TTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGA                                                                                  <  SRR4302542.1652080/94‑1 (MQ=60)
            TTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGA                                                                                  <  SRR4302542.1380772/94‑1 (MQ=60)
            TTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGA                                                                                  >  SRR4302542.7057879/1‑94 (MQ=60)
             TTCCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAC                                                                                 <  SRR4302542.7306842/94‑1 (MQ=60)
             TTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAC                                                                                 <  SRR4302542.1585240/94‑1 (MQ=60)
             TTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAC                                                                                 <  SRR4302542.4414806/94‑1 (MQ=60)
               ACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                               <  SRR4302542.544540/94‑1 (MQ=60)
               ACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                               <  SRR4302542.3405532/94‑1 (MQ=60)
               ACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                               >  SRR4302542.1052356/1‑94 (MQ=60)
                CCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGA                                                                              >  SRR4302542.946735/1‑94 (MQ=60)
                 CGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                             >  SRR4302542.803327/1‑94 (MQ=60)
                  GGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACA                                                                            >  SRR4302542.1229482/1‑94 (MQ=60)
                    CGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACC                                                                          >  SRR4302542.801376/1‑94 (MQ=60)
                     GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                         >  SRR4302542.309104/1‑94 (MQ=60)
                      TGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAT                                                                        <  SRR4302542.4368645/94‑1 (MQ=60)
                      TGAAGATGGCGCCCCCCAATGAAGATAAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAT                                                                        <  SRR4302542.6338865/94‑1 (MQ=60)
                       GAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATC                                                                       >  SRR4302542.7526720/1‑94 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      <  SRR4302542.82469/94‑1 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      <  SRR4302542.510681/94‑1 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      <  SRR4302542.5096396/94‑1 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      >  SRR4302542.4077880/1‑94 (MQ=60)
                         AGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGA                                                                     >  SRR4302542.3461034/1‑94 (MQ=60)
                          GATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAC                                                                    <  SRR4302542.4954390/94‑1 (MQ=60)
                          GATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAC                                                                    >  SRR4302542.475268/1‑94 (MQ=60)
                           ATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                   <  SRR4302542.6778947/94‑1 (MQ=60)
                             GGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                 >  SRR4302542.3482901/1‑94 (MQ=60)
                              GCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                >  SRR4302542.6858106/1‑94 (MQ=60)
                              GCCCTCGCCCATGAAGATGAATCAGGCATTGGCGAAAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                <  SRR4302542.1296213/94‑1 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302542.4363003/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302542.2091041/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302542.7242987/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302542.3832010/1‑94 (MQ=60)
                                GCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              >  SRR4302542.4686197/1‑94 (MQ=60)
                                GCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              <  SRR4302542.4662331/94‑1 (MQ=60)
                                 CTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGC                                                             >  SRR4302542.2165957/1‑94 (MQ=60)
                                 CTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGC                                                             >  SRR4302542.6478422/1‑94 (MQ=60)
                                  TCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCA                                                            <  SRR4302542.3459248/94‑1 (MQ=60)
                                    GCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATT                                                          >  SRR4302542.3742357/1‑94 (MQ=60)
                                    GCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATT                                                          >  SRR4302542.4908747/1‑94 (MQ=60)
                                      CCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTC                                                        <  SRR4302542.1657600/94‑1 (MQ=60)
                                      CCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTC                                                        <  SRR4302542.6435994/94‑1 (MQ=60)
                                       CATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       >  SRR4302542.5341614/1‑94 (MQ=60)
                                       CATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       <  SRR4302542.1902667/94‑1 (MQ=60)
                                       CATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       <  SRR4302542.490909/94‑1 (MQ=60)
                                        ATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                      >  SRR4302542.248093/1‑94 (MQ=60)
                                        ATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                      <  SRR4302542.655255/94‑1 (MQ=60)
                                         TGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTT                                                     >  SRR4302542.1050150/1‑94 (MQ=60)
                                          GAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGGCACCATCGACAACGAGCATTTCTTTG                                                    <  SRR4302542.5071599/94‑1 (MQ=60)
                                             GATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGC                                                 >  SRR4302542.3016405/1‑94 (MQ=60)
                                              ATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCG                                                >  SRR4302542.2198661/1‑94 (MQ=60)
                                                 AATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAA                                             <  SRR4302542.6603731/94‑1 (MQ=60)
                                                  ATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAG                                            >  SRR4302542.989097/1‑94 (MQ=60)
                                                  ATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAG                                            >  SRR4302542.814273/1‑94 (MQ=60)
                                                  ATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAG                                            <  SRR4302542.4522274/94‑1 (MQ=60)
                                                  ATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAG                                            <  SRR4302542.2412561/94‑1 (MQ=60)
                                                   TCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                           <  SRR4302542.5866756/94‑1 (MQ=60)
                                                    CAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAA                                          >  SRR4302542.4553726/1‑94 (MQ=60)
                                                    CAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAA                                          <  SRR4302542.539398/94‑1 (MQ=60)
                                                     AGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAT                                         <  SRR4302542.5764812/94‑1 (MQ=60)
                                                     AGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAT                                         >  SRR4302542.3599925/1‑94 (MQ=60)
                                                     AGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAT                                         <  SRR4302542.207192/94‑1 (MQ=60)
                                                     AGGCATGGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAT                                         <  SRR4302542.1291694/94‑1 (MQ=60)
                                                      GGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATG                                        >  SRR4302542.3723033/1‑94 (MQ=60)
                                                      GGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATG                                        <  SRR4302542.3180813/94‑1 (MQ=60)
                                                      GGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATG                                        <  SRR4302542.3604488/94‑1 (MQ=60)
                                                        CATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGA                                      >  SRR4302542.6674046/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.679129/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.2766613/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.307556/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.3226605/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.908722/1‑94 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     <  SRR4302542.1087942/94‑1 (MQ=60)
                                                         ATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     >  SRR4302542.4989804/1‑94 (MQ=60)
                                                          TTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATT                                    >  SRR4302542.4677299/1‑94 (MQ=60)
                                                          TTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATT                                    <  SRR4302542.1571413/94‑1 (MQ=60)
                                                           TGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                   <  SRR4302542.4332054/94‑1 (MQ=60)
                                                           TGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                   <  SRR4302542.3546622/94‑1 (MQ=60)
                                                            GGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGC                                  <  SRR4302542.7150680/94‑1 (MQ=60)
                                                             GCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCT                                 <  SRR4302542.6611412/94‑1 (MQ=60)
                                                             GCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCT                                 <  SRR4302542.6235442/94‑1 (MQ=60)
                                                              CGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTG                                <  SRR4302542.4028611/94‑1 (MQ=60)
                                                              CGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTG                                >  SRR4302542.53490/1‑94 (MQ=60)
                                                               GACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGT                               <  SRR4302542.3683844/94‑1 (MQ=60)
                                                               GACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGT                               >  SRR4302542.4860027/1‑94 (MQ=60)
                                                                ACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTA                              <  SRR4302542.7114043/94‑1 (MQ=60)
                                                                ACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTA                              <  SRR4302542.6678048/94‑1 (MQ=60)
                                                                 CAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAC                             >  SRR4302542.6162502/1‑94 (MQ=60)
                                                                 CAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAC                             >  SRR4302542.69329/1‑94 (MQ=60)
                                                                  AAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACG                            <  SRR4302542.4396846/94‑1 (MQ=60)
                                                                  AAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACG                            >  SRR4302542.1651414/1‑94 (MQ=60)
                                                                  AAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACG                            <  SRR4302542.915070/94‑1 (MQ=60)
                                                                   agcCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGC                           >  SRR4302542.7551543/4‑94 (MQ=60)
                                                                    gcCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCC                          <  SRR4302542.2654391/92‑1 (MQ=60)
                                                                    gcCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCC                          <  SRR4302542.3177881/92‑1 (MQ=60)
                                                                    C‑CGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCA                         >  SRR4302542.2488423/1‑94 (MQ=60)
                                                                      CGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                        >  SRR4302542.379420/1‑94 (MQ=60)
                                                                       GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       >  SRR4302542.5921444/1‑94 (MQ=60)
                                                                       GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       >  SRR4302542.6640590/1‑94 (MQ=60)
                                                                       GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       <  SRR4302542.1710112/94‑1 (MQ=60)
                                                                          GGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCG                    <  SRR4302542.7275667/94‑1 (MQ=60)
                                                                            TGCGCTGGTTACGC‑GTTCTTTTGCGCATGACCGACACCATCGACAACGAGCATTTTTTTGTGCGTAAAAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302542.3137448/94‑1 (MQ=60)
                                                                            TGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302542.3720321/94‑1 (MQ=60)
                                                                            TGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302542.5811084/94‑1 (MQ=60)
                                                                             GCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTG                 <  SRR4302542.2096007/94‑1 (MQ=60)
                                                                              CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                >  SRR4302542.1416158/1‑94 (MQ=60)
                                                                               GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAT               <  SRR4302542.6547655/94‑1 (MQ=60)
                                                                                CTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG              <  SRR4302542.1637416/94‑1 (MQ=60)
                                                                                CTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG              <  SRR4302542.569797/94‑1 (MQ=60)
                                                                                 TGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGC             <  SRR4302542.1267697/94‑1 (MQ=60)
                                                                                 TGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGC             <  SRR4302542.6714453/94‑1 (MQ=60)
                                                                                  GGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCT            <  SRR4302542.7167240/94‑1 (MQ=60)
                                                                                   GCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTG           <  SRR4302542.3019372/94‑1 (MQ=60)
                                                                                    CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          >  SRR4302542.6772929/1‑94 (MQ=60)
                                                                                    CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          <  SRR4302542.5945714/94‑1 (MQ=60)
                                                                                     TACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT         >  SRR4302542.6828469/1‑94 (MQ=60)
                                                                                     TACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT         >  SRR4302542.2581921/1‑94 (MQ=60)
                                                                                      ACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTA        <  SRR4302542.5450278/94‑1 (MQ=60)
                                                                                        GCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTAAT      <  SRR4302542.4919129/94‑1 (MQ=60)
                                                                                         CGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTG     <  SRR4302542.1830807/94‑1 (MQ=60)
                                                                                            GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT   <  SRR4302542.2024604/94‑1 (MQ=60)
                                                                                            GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT   <  SRR4302542.2185109/94‑1 (MQ=60)
                                                                                             TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  <  SRR4302542.6575604/94‑1 (MQ=60)
                                                                                             TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  <  SRR4302542.6083484/94‑1 (MQ=60)
                                                                                             TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  SRR4302542.5298149/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  NC_000913/3236666‑3236851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑