Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,236,758 Δ1 bp coding (537/966 nt) ygjT → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,7570T.99.6% 2344.9 / 2.0 495coding (536/966 nt)ygjTputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (256/235);  minor base G (2/0);  total (258/237)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 541 total aligned reads displayed.

GGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTG  >  NC_000913/3236663‑3236848
                                                                                               |                                                                                           
ggTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGt                                                                                             >  2:935123/1‑96 (MQ=255)
 gTGCCTTCCTGCAGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtc                                                                                            <  2:12045913/96‑2 (MQ=255)
   gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtcat                                                                                          >  1:12823361/1‑93 (MQ=255)
    ccTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGGtcatt                                                                                         <  2:4633211/96‑5 (MQ=255)
       tCCTGCTGTTTCCCGGGGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGCCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                     <  2:3583009/96‑1 (MQ=255)
       tCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                     <  2:6776430/96‑1 (MQ=255)
       tCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACG‑GGGTCATTTgc                                                                                     >  2:643240/1‑96 (MQ=255)
        ccTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgcg                                                                                    >  1:3136291/1‑96 (MQ=255)
         ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgcgc                                                                                   >  2:8443363/1‑96 (MQ=255)
         ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTgcgc                                                                                   >  2:712535/1‑96 (MQ=255)
          tgctgTTTACCGGCGTGAAGATGGCGCTCCCCCATGAAGATGAATCAGTCATTGCCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCa                                                                                  <  2:9833997/96‑1 (MQ=255)
           gctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAt                                                                                 >  2:7449859/1‑96 (MQ=255)
           gctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAt                                                                                 >  1:14044593/1‑96 (MQ=255)
            ctgTTTACCGGCGTTAAGATGGCGCTCGCCCATGAAGATGAATCAGGCTTTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATg                                                                                <  1:7959432/96‑1 (MQ=255)
            ctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATg                                                                                >  2:586465/1‑96 (MQ=255)
             tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGa                                                                               >  1:13337679/1‑96 (MQ=255)
              gTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAc                                                                              >  2:13547728/1‑96 (MQ=255)
               tttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                             >  2:2591582/1‑96 (MQ=255)
               tttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                             <  2:5983091/96‑1 (MQ=255)
               tttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                             >  2:7870669/1‑96 (MQ=255)
               tttACCGGCGGGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                             <  2:11073994/96‑1 (MQ=255)
                ttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGTCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCg                                                                            <  2:3750418/96‑1 (MQ=255)
                 tACCGGCGTGAATATGGAGCACGCCCATGAAGATGATGCAGGCATTGGCCACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGa                                                                           <  1:5611381/96‑1 (MQ=255)
                    gggCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGacac                                                                        >  1:8866431/2‑96 (MQ=255)
                     ggCGTGAAGATGGCGCTCGCCCATGAAGATGAACCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACAcc                                                                       <  2:1932117/96‑1 (MQ=255)
                      gCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCa                                                                      >  2:8100280/1‑96 (MQ=255)
                      gCGTCAAGATGGCGCTCGCCCATGAAGTTTAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCa                                                                      <  1:11893931/96‑1 (MQ=255)
                        gTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                    >  1:12462863/1‑96 (MQ=255)
                          gAAGATGGCCCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGa                                                                  <  2:4468833/96‑1 (MQ=255)
                           aaGATGGCGCTCGCCCTTGAAGATGAATAAGGCATTGGCACCAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAc                                                                 <  1:8693198/96‑1 (MQ=255)
                           aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAc                                                                 <  2:1047977/96‑1 (MQ=255)
                             gATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACaa                                                               >  1:12999649/1‑96 (MQ=255)
                              aTGGCGCTGGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                              <  1:9803557/96‑1 (MQ=255)
                              aTGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                              <  1:5838543/96‑1 (MQ=255)
                              aTGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                              >  2:10624421/1‑96 (MQ=255)
                              aTGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                              <  1:1206650/96‑1 (MQ=255)
                               tGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                             <  1:8493531/96‑1 (MQ=255)
                               tGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                             >  2:6123891/1‑96 (MQ=255)
                               tGGCGCTCGCCCATGAAGATAAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                             <  1:2708352/96‑1 (MQ=255)
                                ggCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                            >  2:11550853/1‑96 (MQ=255)
                                   gcTCACCCATGAAGATGAGTCAGGCATTGGCGACAAGCCTCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATTACCGACACCATCGACAACGAGCa                                                         >  1:10140900/1‑96 (MQ=255)
                                    cTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAt                                                        >  1:4517072/1‑96 (MQ=255)
                                      cGCCCATGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                      >  2:9566901/1‑96 (MQ=255)
                                          cacgAAGATAAATCAGGCATTGGCGAAAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGCCACCATCGACAACGAGCATTTCttt                                                  <  1:13763221/93‑1 (MQ=255)
                                           aTGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtg                                                 >  1:13911498/1‑96 (MQ=255)
                                            tGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgt                                                <  1:9050671/96‑1 (MQ=255)
                                            tGAAGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgt                                                >  2:6063150/1‑96 (MQ=255)
                                              aaGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGc                                              >  1:7070993/1‑96 (MQ=255)
                                               aGATGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCg                                             >  2:14164271/1‑96 (MQ=255)
                                                 aTGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTa                                           >  2:1742908/1‑96 (MQ=255)
                                                  tGAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTaa                                          >  2:1373595/1‑96 (MQ=255)
                                                   gAATCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAg                                         <  1:4740711/96‑1 (MQ=255)
                                                    aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGa                                        <  1:3442833/96‑1 (MQ=255)
                                                    aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGa                                        <  1:7790609/96‑1 (MQ=255)
                                                    aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGa                                        >  1:7389996/1‑96 (MQ=255)
                                                    aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAGTTCTTTGTGCGTAAGa                                        >  1:10328510/1‑96 (MQ=255)
                                                    aaTCAGGCATTGGCGACAAGCCGCTGGTGCGCTCGCTACGCGG‑CCATTTGCGCATGACCGACACCAACGACAACGAGCATTTCTGTGTGCGTAAGa                                        >  2:7720737/1‑96 (MQ=255)
                                                     aTCAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACTTGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGACTTTCTTTTTGCGTAAGaa                                       >  1:6583786/1‑96 (MQ=255)
                                                       cAGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATg                                     >  2:5284212/1‑96 (MQ=255)
                                                       aagtcATTGGCGACAAGCCGCTAGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGCCAACGAGCATTTCTTTGTGCGTAAGAATg                                     <  1:9451016/92‑1 (MQ=255)
                                                        aGGCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATgc                                    <  1:11330317/96‑2 (MQ=255)
                                                         ggCTTTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGa                                   <  2:229020/96‑1 (MQ=255)
                                                         ggCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGCCCGCCACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGa                                   <  1:1635422/96‑1 (MQ=255)
                                                          gCATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                                  <  2:1488377/96‑1 (MQ=255)
                                                           cgttGGCGACAAGCCGCTGGTGCCCGGGCTCCGCGG‑TCATTTGCGCATGACCGACGCCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAtt                                 <  1:4165738/94‑1 (MQ=255)
                                                           cATTGGCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAtt                                 >  1:8211996/1‑96 (MQ=255)
                                                               ggCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTg                             <  2:4994701/96‑1 (MQ=255)
                                                               ggCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTg                             <  2:13668430/96‑1 (MQ=255)
                                                                gCGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGACTGCTGt                            <  2:11241170/96‑1 (MQ=255)
                                                                 cGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTa                           >  2:1409362/1‑96 (MQ=255)
                                                                 cGACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGAGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTa                           <  2:12250055/96‑1 (MQ=255)
                                                                  gACAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAc                          <  2:3020777/96‑1 (MQ=255)
                                                                   aCAAGCCGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACg                         <  1:1816428/96‑1 (MQ=255)
                                                                         cgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTTCGCCacac                   <  2:12974650/96‑1 (MQ=255)
                                                                         cgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac                   >  1:9309396/1‑96 (MQ=255)
                                                                         cgcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac                   >  1:7141676/1‑96 (MQ=255)
                                                                         cgcTGGTGCGCTGGCTACGCGG‑TCATTAGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac                   >  2:12707190/1‑96 (MQ=255)
                                                                          gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACAcc                  >  1:613846/1‑96 (MQ=255)
                                                                           cgggTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCg                 >  1:14266898/3‑96 (MQ=255)
                                                                            tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc                >  2:7282131/1‑96 (MQ=255)
                                                                            tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc                >  2:12474519/1‑96 (MQ=255)
                                                                            tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc                >  1:2992807/1‑96 (MQ=255)
                                                                              gTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTg              >  1:6318491/1‑96 (MQ=255)
                                                                              gTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTg              >  2:4070649/1‑96 (MQ=255)
                                                                              gTGCGCTCGCTACGCGG‑TCATTTCCGCATGACCGACGCCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTg              <  2:4368597/96‑1 (MQ=255)
                                                                                  gcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGTTGc          >  1:2159615/1‑96 (MQ=255)
                                                                                   cTGGCTACGCGG‑TCATTTGCGCATGCCCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCt         <  2:13287880/96‑1 (MQ=255)
                                                                                    tGGCTACGCGG‑TCATTTGCGCATGACCGACCCCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTg        <  2:5677938/96‑1 (MQ=255)
                                                                                     ggCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTgg       >  2:904754/1‑96 (MQ=255)
                                                                                      gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTggg      >  1:292261/1‑95 (MQ=255)
                                                                                      gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt      <  1:5005313/96‑1 (MQ=255)
                                                                                      gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt      <  1:10751675/96‑1 (MQ=255)
                                                                                      gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCCCTGATGCTGGt      <  2:8696895/96‑1 (MQ=255)
                                                                                        tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTAc    >  1:5425874/1‑96 (MQ=255)
                                                                                        tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTAc    >  1:3577126/1‑96 (MQ=255)
                                                                                        tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTAc    >  1:11645443/1‑96 (MQ=255)
                                                                                         aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACt   <  1:13548426/96‑1 (MQ=255)
                                                                                         aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACt   >  2:10412922/1‑96 (MQ=255)
                                                                                         aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACt   <  2:3410255/96‑1 (MQ=255)
                                                                                          cgcgG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTg  >  2:10164279/1‑96 (MQ=255)
                                                                                               |                                                                                           
GGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CGCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTG  >  NC_000913/3236663‑3236848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 303 total aligned reads displayed.
CTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑G‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGG  >  NC_000913/3236659‑3236858
                                                                                                      |                                                                                                    
CTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGNNN                                                                                                      >  SRR4302518.11780780/1‑101 (MQ=60)
   CGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATT                                                                                                  >  SRR4302518.5658440/1‑101 (MQ=60)
    NNNGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                                 <  SRR4302518.14915199/101‑1 (MQ=60)
    GGTGTCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                                 <  SRR4302518.6001092/101‑1 (MQ=60)
    GGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                                 >  SRR4302518.939577/1‑101 (MQ=60)
    GGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                                 <  SRR4302518.5276832/101‑1 (MQ=60)
    GGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGATCGCCCATGAAGATGAATCAGGCATTGGCGACAACC‑C‑GCTGGTGCGCTGGCTACGCGG‑TCATTT                                                                                                 >  SRR4302518.487722/1‑101 (MQ=60)
      TGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                               >  SRR4302518.5934732/1‑101 (MQ=60)
        NNNTCCTGCTGTTTACGGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGC                                                                                             <  SRR4302518.8850231/101‑1 (MQ=60)
         NNNCCTGCTGTTTCCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCA                                                                                            <  SRR4302518.10343093/101‑1 (MQ=60)
         CTTCCTGCTGTTTACCGGCGTGAGGATGGCGCTCGCCCATGAAGATGAGTCAGGCATTGGCGACAA‑G‑CGGCTGGTGCGCTGGCTACGCGG‑TCATTTGCNNN                                                                                            >  SRR4302518.9848435/1‑101 (MQ=60)
          TTCCTGCTGTTTACAGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                           <  SRR4302518.3297823/101‑1 (MQ=60)
           NNNTGCTGTTTACCGGCGTTAAGATGGCGCTCGCCCATGAAGATGAATCAGGCTTTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATG                                                                                          <  SRR4302518.8357336/101‑1 (MQ=60)
           NNNTGCTGTTTACCGGCGTGAAGATGGCCCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAGG                                                                                          <  SRR4302518.10779580/101‑1 (MQ=60)
            NNNGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGA                                                                                         <  SRR4302518.13355904/101‑1 (MQ=60)
            CCTGCTGTTTCCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGA                                                                                         <  SRR4302518.4777184/101‑1 (MQ=60)
            CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCCTGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGC                                                                                         >  SRR4302518.1354885/1‑101 (MQ=60)
             CTCCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAC                                                                                        <  SRR4302518.3218645/101‑1 (MQ=60)
              NNNTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACC                                                                                       <  SRR4302518.8345489/101‑1 (MQ=60)
               GCTGTTTCCCGGCGTGAAGATGGCGCTCGCCCAGGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                                      <  SRR4302518.814596/101‑1 (MQ=60)
               GCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGANNN                                                                                      >  SRR4302518.14687499/1‑101 (MQ=60)
                CTTTTTACCGGCGTGAATATGGAGCACGCCCATGAAGATGATGCAGGCATTGGCCACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGA                                                                                     <  SRR4302518.5895893/101‑1 (MQ=58)
                CTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGC                                                                                     >  SRR4302518.812092/1‑101 (MQ=60)
                 TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCNNN                                                                                    >  SRR4302518.13951526/1‑101 (MQ=60)
                 TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCNNN                                                                                    >  SRR4302518.7776573/1‑101 (MQ=60)
                 TGTTTACCGGCGTGAAGATGGCGCGCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                                    <  SRR4302518.1353583/101‑1 (MQ=60)
                 TGTGTAGCGGCGTGAAGGTGGCGCTCGCCCATGAAGATGAATCAGGGATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                                    <  SRR4302518.3042958/101‑1 (MQ=60)
                 NNNTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                                    <  SRR4302518.12595175/101‑1 (MQ=60)
                 NNNTTACCGGCGTGAAGATGGCGCTCGCCAATGAAAATAAATCAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                                    <  SRR4302518.9199105/101‑1 (MQ=60)
                  nnnaacggggctacagatgagactcacccaaaaaaataaaaCAGGCATTGGCGACAA‑GCC‑GCTCGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACA                                                                                   <  SRR4302518.14962868/60‑1 (MQ=52)
                  NNNTACCGGCGTGAAGATGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACA                                                                                   <  SRR4302518.10337647/101‑1 (MQ=60)
                   TTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGANNN                                                                                  >  SRR4302518.14421696/1‑101 (MQ=60)
                    ttaccggcgttacagTGGCGCGCGCCCATGAAGATGAATCGGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACCCC                                                                                 <  SRR4302518.6766133/86‑1 (MQ=60)
                    TTACCGGCGTGAAGATGGCGCTGGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACC                                                                                 <  SRR4302518.175289/101‑1 (MQ=60)
                     TACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACANNN                                                                                >  SRR4302518.12181271/1‑101 (MQ=60)
                     NNNCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                                <  SRR4302518.14526730/101‑1 (MQ=60)
                     NNNCGGCGTCAAGATGGCGCTCGCCCATGAAGTTTAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                                <  SRR4302518.12460203/101‑1 (MQ=60)
                      nnnaggctcaaagtgaccgttcgcccatgaaaataattcaggcattgccgaatagcC‑GCTGGTTCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                                <  SRR4302518.9388564/45‑1 (MQ=60)
                        cggctggatgcTGGCGCTCGCCCATGAAGATGAATGAGGCATGGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                             <  SRR4302518.2891313/90‑1 (MQ=60)
                        GGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCANNN                                                                             >  SRR4302518.9296326/1‑101 (MQ=60)
                          NNNTGAGGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAC                                                                           <  SRR4302518.10973477/101‑1 (MQ=60)
                          NNNTGAAGATGGCGCTCGCCCTTGAAGATGAATAAGGCATTGGCACCAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAC                                                                           <  SRR4302518.9115910/101‑1 (MQ=60)
                          GCGGGAAGAGGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAC                                                                           <  SRR4302518.3624169/101‑1 (MQ=60)
                           NNNGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                          <  SRR4302518.12289972/101‑1 (MQ=60)
                           NNNGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                          <  SRR4302518.12297126/101‑1 (MQ=60)
                            NNNAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAA                                                                         <  SRR4302518.13943576/101‑1 (MQ=60)
                            GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGANNN                                                                         >  SRR4302518.13049244/1‑101 (MQ=60)
                            GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAC                                                                         >  SRR4302518.7275851/1‑101 (MQ=60)
                            GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAA                                                                         >  SRR4302518.3070257/1‑101 (MQ=60)
                            GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAA                                                                         >  SRR4302518.3415519/1‑101 (MQ=60)
                             TGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                        >  SRR4302518.6572851/1‑101 (MQ=60)
                             TGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                        <  SRR4302518.6134315/101‑1 (MQ=60)
                             TGAAGATGGCGCTCGACCAGGAAGATGAATCAGGCCTTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                        <  SRR4302518.6359717/101‑1 (MQ=60)
                             TGAAGATGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                        <  SRR4302518.1255418/101‑1 (MQ=60)
                             NNNAGATGGCGCCCGCCCATGAAGATGAATCAGGCATTGGCTACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAC                                                                        <  SRR4302518.9407888/101‑1 (MQ=60)
                              NNNGATGGTGCTCGCCCATGAAGATGAATCAGGCATGGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                       <  SRR4302518.14995058/101‑1 (MQ=60)
                              GAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                       >  SRR4302518.3182338/1‑101 (MQ=60)
                              GAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                       >  SRR4302518.908104/1‑101 (MQ=60)
                              GAAGATGGCGCTCGCCCATGAAGATAAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                       <  SRR4302518.2837252/101‑1 (MQ=60)
                               AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGGGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑CCGTTTGCGCATCACCGACACCATCGACAACGC                                                                      >  SRR4302518.6393207/1‑101 (MQ=60)
                               AAGAGCGCGCTCGCCCATGACGCTGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                                      <  SRR4302518.2034428/101‑1 (MQ=60)
                                nnntggcgcccgcactTGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                                     <  SRR4302518.7625513/85‑1 (MQ=60)
                                  nnnnnnnnnnnnnnnntnannnnnnnncnnncantgnnnncnagnnGCNGNTGCGCNGGNTNCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                                     <  SRR4302518.14835664/55‑1 (MQ=49)
                                    nnngctagaccaggcagagaacgCAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAAAGACAACGAGCATC                                                                 <  SRR4302518.11948991/78‑1 (MQ=57)
                                    GGCGCTCGCCCATGAAGTGCAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATT                                                                 <  SRR4302518.2006427/101‑1 (MQ=60)
                                     NNNCTCCCCCAGGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTT                                                                <  SRR4302518.8558230/101‑1 (MQ=60)
                                      CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATNNN                                                               >  SRR4302518.14346513/1‑101 (MQ=60)
                                       nnncccccataaaaATGAAACAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCC                                                              <  SRR4302518.11864074/87‑1 (MQ=60)
                                       ccaccccgataAAGATGAATCAGGAATGGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                              <  SRR4302518.3372728/90‑1 (MQ=60)
                                       GCTCACCCATGAAGATGAGTCAGGCATTGGCGACAA‑GCC‑TCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATTACCGACACCATCGACAACGAGCATTNNN                                                              >  SRR4302518.10620197/1‑101 (MQ=60)
                                        NNNGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                             <  SRR4302518.8049445/101‑1 (MQ=60)
                                        NNNGCCCATGAAGATGAATCAGGAATTGGCTACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTC                                                             <  SRR4302518.11853518/101‑1 (MQ=60)
                                        NNNGCCCAGGAAGATAAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACCACGAGCATTTCTT                                                             <  SRR4302518.14488991/101‑1 (MQ=60)
                                        CTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                             >  SRR4302518.5247451/1‑101 (MQ=60)
                                        CTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                             >  SRR4302518.4752288/1‑101 (MQ=60)
                                        CTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAGTTCTT                                                             >  SRR4302518.6452632/1‑101 (MQ=60)
                                         nnncacacGAAGATAAATCAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGCCACCATCGACAACGAGCATTTCTTT                                                            <  SRR4302518.14393298/93‑1 (MQ=60)
                                         cttCCCATGAAGATAAATCAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGCCCGACACCATCGACAACGAGCATTTCTTT                                                            <  SRR4302518.3712047/98‑1 (MQ=60)
                                         TCGCCCGTGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTT                                                            <  SRR4302518.2493240/101‑1 (MQ=60)
                                         TAGCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTT                                                            <  SRR4302518.4643202/101‑1 (MQ=60)
                                         CCGCCCATGAAAATGAATCGGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTT                                                            <  SRR4302518.6458539/101‑1 (MQ=60)
                                          NNNCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCGGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTG                                                           <  SRR4302518.10258343/101‑1 (MQ=60)
                                          CGCCCATGAAGAGGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTG                                                           <  SRR4302518.4217750/101‑1 (MQ=60)
                                           NNNCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGT                                                          <  SRR4302518.9488052/101‑1 (MQ=60)
                                           GCCCATGAAGATGAATCAGGCGTTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGT                                                          <  SRR4302518.7061389/101‑1 (MQ=60)
                                           GCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCCTCGACAACGAGCATTTCTTTGT                                                          >  SRR4302518.1339382/1‑101 (MQ=60)
                                           GCCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGT                                                          >  SRR4302518.5254100/1‑101 (MQ=60)
                                           GCCCATGAAGAGGAATCAGGCATTGGCGACAA‑GCC‑GCTGGAGCGCTGGCTACGCGG‑TCATTTTCGCAGGACCGACACCACCGACAACTAGCATTTCTTAGT                                                          >  SRR4302518.6724103/1‑101 (MQ=58)
                                            NNNATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCTCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTG                                                         <  SRR4302518.7717660/101‑1 (MQ=60)
                                            CCCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTNNN                                                         >  SRR4302518.10464456/1‑101 (MQ=60)
                                             nnnatgaatataaataagcaattggagacaagcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGGG                                                         <  SRR4302518.7904773/68‑1 (MQ=60)
                                             CCATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGC                                                        >  SRR4302518.3683884/1‑101 (MQ=60)
                                              cgcaggaggatgtgtggggtattgcgatgaagcgGCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGCCCGCCACCATCGACAACGAGCCTTTCTTTGTG                                                         <  SRR4302518.5756972/67‑1 (MQ=60)
                                              CATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTNNN                                                       >  SRR4302518.9522188/1‑101 (MQ=60)
                                               ccgatctTGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGT                                                      <  SRR4302518.6140274/94‑1 (MQ=60)
                                               ATGAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGNNN                                                      >  SRR4302518.14548099/1‑101 (MQ=60)
                                                TGAAGACGAATCCGGCCTTGGCGCCAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTA                                                     <  SRR4302518.7027028/101‑1 (MQ=60)
                                                 GAAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAA                                                    >  SRR4302518.495598/1‑101 (MQ=60)
                                                  ATGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTNNN                                                   >  SRR4302518.12030535/1‑101 (MQ=60)
                                                  AAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTNNN                                                   >  SRR4302518.7444662/1‑101 (MQ=60)
                                                  AAGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAG                                                   <  SRR4302518.4989376/101‑1 (MQ=60)
                                                   NNNTGACTCAGGCAGTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                                  <  SRR4302518.11338263/101‑1 (MQ=60)
                                                   NNNTGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                                  <  SRR4302518.8184081/101‑1 (MQ=60)
                                                   AGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTANNN                                                  >  SRR4302518.14177431/1‑101 (MQ=60)
                                                   AGATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                                  <  SRR4302518.3106374/101‑1 (MQ=60)
                                                     nnnaaaatcaggaataggcgaaaaaccgctaGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                                  <  SRR4302518.7738919/70‑1 (MQ=60)
                                                     ATGAATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGNNN                                                >  SRR4302518.12589078/1‑101 (MQ=60)
                                                      agaaatgcggtctgggagaaaagcC‑GCTGGTGCGCTGGCCACGCGG‑TCATTT‑‑GCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                              <  SRR4302518.877359/77‑1 (MQ=60)
                                                       taataAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                              <  SRR4302518.3133819/96‑1 (MQ=60)
                                                       GAATCAGGCATTGGCGCCAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                              <  SRR4302518.3289795/101‑1 (MQ=60)
                                                        AATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGCCCGCCACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGA                                             <  SRR4302518.1707164/101‑1 (MQ=60)
                                                        AATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATNNN                                             >  SRR4302518.14176119/1‑101 (MQ=60)
                                                        AATCAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAGTTCTTTGTGCGTAAGAATNNN                                             >  SRR4302518.10814770/1‑101 (MQ=60)
                                                          ntcggcgTTGGCGACAA‑GCC‑GCTGGTGCCCGGGCTCCGCGG‑TCATTTGCGCATGACCGACGCCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATT                                           <  SRR4302518.4368083/94‑1 (MQ=60)
                                                          TAAGGCATTGGCGAAAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATT                                           <  SRR4302518.971249/101‑1 (MQ=60)
                                                           nnngtATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                          <  SRR4302518.9801692/96‑1 (MQ=60)
                                                           nnngctTTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                          <  SRR4302518.9059751/95‑1 (MQ=60)
                                                           CGGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                          <  SRR4302518.6361186/101‑1 (MQ=60)
                                                           CAGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGANNN                                          >  SRR4302518.12814763/1‑101 (MQ=60)
                                                            AGGCATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGC                                         <  SRR4302518.5833816/101‑1 (MQ=60)
                                                               NNNTGGCGACAA‑GCC‑GCTGGTGCGCTGGATACGC‑GATCATTCGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTCT                                      <  SRR4302518.10742972/101‑1 (MQ=60)
                                                               CATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGT                                      >  SRR4302518.2912555/1‑101 (MQ=60)
                                                               CATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCNNN                                      >  SRR4302518.8617689/1‑101 (MQ=60)
                                                               CATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTCCTTTGTGCGTAAGAATGGATTGCTGT                                      <  SRR4302518.7315140/101‑1 (MQ=60)
                                                                TTTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTA                                     <  SRR4302518.1180631/101‑1 (MQ=60)
                                                                NTTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCGGTN                                     >  SRR4302518.2840749/1‑101 (MQ=60)
                                                                ATTGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACG‑GGGACATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTNNN                                     >  SRR4302518.12429788/1‑101 (MQ=60)
                                                                ATTGGCGACAA‑G‑CGGCTGGGGCGCTGGCTACGCGG‑GCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGAGTAAGAATGGATGGCTNNN                                     >  SRR4302518.9521855/1‑101 (MQ=60)
                                                                  TGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTNNN                                   >  SRR4302518.9017668/1‑101 (MQ=60)
                                                                  TGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACG                                   <  SRR4302518.1898036/101‑1 (MQ=60)
                                                                  TGGCGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACC                                   >  SRR4302518.4144699/1‑101 (MQ=60)
                                                                   cttgcaaaaagccgctaGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAC                                    <  SRR4302518.550799/84‑1 (MQ=60)
                                                                     nnncaaagcC‑GCTGGTGCGCTGTCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCC                                 <  SRR4302518.13817267/92‑1 (MQ=60)
                                                                     CGACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCA                                >  SRR4302518.2222552/1‑101 (MQ=60)
                                                                      GACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                               >  SRR4302518.45423/1‑101 (MQ=60)
                                                                      GACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                               >  SRR4302518.5776057/1‑101 (MQ=60)
                                                                      GACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                               <  SRR4302518.2398362/101‑1 (MQ=60)
                                                                       nnnaagcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTCTACGCCCC                               <  SRR4302518.13573715/94‑1 (MQ=60)
                                                                       ACAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCNNN                              >  SRR4302518.9969444/1‑101 (MQ=60)
                                                                        nnnagcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                              <  SRR4302518.14608657/95‑1 (MQ=60)
                                                                        CAA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCaannn                             >  SRR4302518.14931701/1‑96 (MQ=60)
                                                                         nnngcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACAC                             <  SRR4302518.10238413/96‑1 (MQ=60)
                                                                         NN‑N‑C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCG                           <  SRR4302518.13484806/101‑1 (MQ=60)
                                                                         AA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACNNN                            >  SRR4302518.10152813/1‑101 (MQ=60)
                                                                         AA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACNNN                            >  SRR4302518.7469050/1‑101 (MQ=60)
                                                                         AA‑GCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACC                            <  SRR4302518.581987/101‑1 (MQ=60)
                                                                         AA‑GCC‑GCTCGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTCCGCCCCACC                            <  SRR4302518.933637/101‑1 (MQ=60)
                                                                          nnncC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACC                            <  SRR4302518.13519910/97‑1 (MQ=60)
                                                                           agcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCG                           >  SRR4302518.1538650/4‑101 (MQ=60)
                                                                            gcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACNNN                          >  SRR4302518.14122368/3‑101 (MQ=60)
                                                                            N‑N‑NCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                         <  SRR4302518.12698641/101‑1 (MQ=60)
                                                                            C‑C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCNNN                         >  SRR4302518.8604137/1‑101 (MQ=60)
                                                                              C‑TCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTG                        <  SRR4302518.1744859/101‑1 (MQ=60)
                                                                              C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGNNN                        >  SRR4302518.14082449/1‑101 (MQ=60)
                                                                              C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGNNN                        >  SRR4302518.9758550/1‑101 (MQ=60)
                                                                              C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTG                        >  SRR4302518.6790518/1‑101 (MQ=60)
                                                                              C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACAAGCATTTCTTTGTGCGTAAGAAATGATTGCTGTACGCAACACCGNNN                        >  SRR4302518.14803161/1‑101 (MQ=60)
                                                                                GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                       >  SRR4302518.4895372/1‑101 (MQ=60)
                                                                                GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                       >  SRR4302518.637635/1‑101 (MQ=60)
                                                                                GCTGGTGCGCTGGCTACGCGG‑GCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTCCGCCACACCGCNNN                       >  SRR4302518.8536597/1‑101 (MQ=60)
                                                                                 NNNGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAT                      <  SRR4302518.14620086/101‑1 (MQ=60)
                                                                                  TGGTGCTCTCGCTACGCGG‑TCATTTGCGCATGCCCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG                     <  SRR4302518.4007746/101‑1 (MQ=60)
                                                                                  TGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG                     >  SRR4302518.312088/1‑101 (MQ=60)
                                                                                  TGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG                     >  SRR4302518.3080449/1‑101 (MQ=60)
                                                                                   NNNGCGCTGGCTCCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGC                    <  SRR4302518.14758043/101‑1 (MQ=60)
                                                                                   GGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGANNN                    >  SRR4302518.7551940/1‑101 (MQ=60)
                                                                                   GGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGANNN                    >  SRR4302518.11017036/1‑101 (MQ=60)
                                                                                    ggctGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATNNN                   >  SRR4302518.8759012/5‑101 (MQ=60)
                                                                                    NNNCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACATCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCT                   <  SRR4302518.9807844/101‑1 (MQ=60)
                                                                                    GTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCG                   >  SRR4302518.6640619/1‑101 (MQ=60)
                                                                                      GCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG                 >  SRR4302518.1921638/1‑101 (MQ=60)
                                                                                       NNNTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACCCCGCTGATGCTGGT                <  SRR4302518.14689242/101‑1 (MQ=60)
                                                                                       NNNTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT                <  SRR4302518.11256723/101‑1 (MQ=60)
                                                                                       NNNTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT                <  SRR4302518.7376732/101‑1 (MQ=60)
                                                                                       CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGTGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT                >  SRR4302518.6871547/1‑101 (MQ=60)
                                                                                       CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT                <  SRR4302518.5265200/101‑1 (MQ=60)
                                                                                        GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGTTGCTAGTA               >  SRR4302518.2262386/1‑101 (MQ=60)
                                                                                        GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTA               >  SRR4302518.5475942/1‑101 (MQ=60)
                                                                                          TGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACT             >  SRR4302518.885948/1‑101 (MQ=60)
                                                                                          TGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTCGTACG             >  SRR4302518.879208/1‑101 (MQ=60)
                                                                                           NNNTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTATGCCACACCGCTGATGCTGGTACTG            <  SRR4302518.8919253/101‑1 (MQ=60)
                                                                                            GCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGGACTGA           >  SRR4302518.304115/1‑101 (MQ=60)
                                                                                             CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCTACACCGCTGATGCTGGTACTNNN          >  SRR4302518.11786423/1‑101 (MQ=60)
                                                                                             CTACGCGG‑TCATTCGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTNNN          >  SRR4302518.14029173/1‑101 (MQ=60)
                                                                                              nnncgcggTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT          <  SRR4302518.9636086/93‑1 (MQ=60)
                                                                                              ccacgcGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAG          >  SRR4302518.3240992/7‑101 (MQ=60)
                                                                                              TACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT         >  SRR4302518.5702843/1‑101 (MQ=60)
                                                                                              TACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT         >  SRR4302518.3744626/1‑101 (MQ=60)
                                                                                               ACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGATTGGATAGCTGTACGCCACACCGCTGATGCTGGTACTGANNN        >  SRR4302518.13317980/1‑101 (MQ=60)
                                                                                               ACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGANNN        >  SRR4302518.7497515/1‑101 (MQ=60)
                                                                                                tcgcggTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTC        <  SRR4302518.4021681/95‑1 (MQ=60)
                                                                                                CGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCT       <  SRR4302518.3729142/101‑1 (MQ=60)
                                                                                                 GCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTG      >  SRR4302518.4537327/1‑101 (MQ=60)
                                                                                                  nnngTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTG      <  SRR4302518.8022106/97‑1 (MQ=60)
                                                                                                  CGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGG     >  SRR4302518.4478976/1‑101 (MQ=60)
                                                                                                  CGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGG     >  SRR4302518.4723775/1‑101 (MQ=60)
                                                                                                    GGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTNNN    >  SRR4302518.10160787/1‑101 (MQ=60)
                                                                                                     GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGNNN   >  SRR4302518.10668880/1‑101 (MQ=60)
                                                                                                      TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGNNN  >  SRR4302518.8724596/1‑101 (MQ=60)
                                                                                                      TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGGACTGATTCTGGNNN  >  SRR4302518.12586221/1‑101 (MQ=60)
                                                                                                      |                                                                                                    
CTTCGGTGCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAA‑G‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATTCTGGTGG  >  NC_000913/3236659‑3236858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑