Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,123 T→C M278V (ATG→GTG)  uxaA ← altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,1230TC83.6% 593.0 / 113.9 269M278V (ATG→GTG) uxaAaltronate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (16/28);  new base C (117/108);  total (133/136)
Fisher's exact test for biased strand distribution p-value = 6.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

Only 100 of 270 total aligned reads displayed.

GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT  >  NC_000913/3240033‑3240215
                                                                                          |                                                                                            
gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                            >  2:24104/1‑93 (MQ=255)
gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                            >  1:1592226/1‑93 (MQ=255)
  aaCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCggg                                                                                          >  1:6690158/1‑93 (MQ=255)
  aaCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCggg                                                                                          <  2:4668311/93‑1 (MQ=255)
   aCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGt                                                                                         >  1:1257139/1‑93 (MQ=255)
    cTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGtt                                                                                        >  2:1576062/1‑93 (MQ=255)
     tGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTa                                                                                       <  1:5846358/93‑1 (MQ=255)
      gCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAg                                                                                      >  1:4925900/1‑93 (MQ=255)
        tctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCa                                                                                    <  1:1301065/93‑1 (MQ=255)
        tctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCa                                                                                    >  2:2610449/1‑93 (MQ=255)
          tGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGt                                                                                  >  1:7066846/1‑93 (MQ=255)
            cgcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTaa                                                                                <  2:1953143/93‑1 (MQ=255)
             gcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAAt                                                                               <  1:2255042/93‑1 (MQ=255)
               cAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAc                                                                             >  1:4306031/1‑93 (MQ=255)
               cAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAc                                                                             >  2:2772295/1‑93 (MQ=255)
               cAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAc                                                                             <  2:2018293/93‑1 (MQ=255)
                aaaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAcc                                                                            >  1:5799852/1‑93 (MQ=255)
                 aaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCa                                                                           >  2:1440918/1‑93 (MQ=255)
                  aCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAg                                                                          <  2:4855908/93‑1 (MQ=255)
                   cATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGa                                                                         >  1:1791343/1‑93 (MQ=255)
                    aTCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGaa                                                                        <  2:645635/93‑1 (MQ=255)
                     tctcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGaaa                                                                       >  1:593686/1‑93 (MQ=255)
                       tcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                     >  1:6290763/1‑93 (MQ=255)
                       tcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                     >  1:4648131/1‑93 (MQ=255)
                         cGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGAcc                                                                   <  2:7200173/93‑1 (MQ=255)
                         cGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGAcc                                                                   >  2:6234801/1‑93 (MQ=255)
                           gCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGt                                                                 <  2:6763204/93‑1 (MQ=255)
                           gCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGCTAGCAGTAATACCAGAAAGACCGt                                                                 >  2:3303973/1‑93 (MQ=255)
                            cACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTc                                                                <  2:1346448/93‑1 (MQ=255)
                            cACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTc                                                                <  2:941388/93‑1 (MQ=255)
                             aCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCa                                                               <  2:3917257/93‑1 (MQ=255)
                             aCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCa                                                               >  2:5955933/1‑93 (MQ=255)
                             aCTTCGATCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCa                                                               <  2:4242836/93‑1 (MQ=255)
                               ttCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGa                                                             >  1:4508360/1‑93 (MQ=255)
                               ttCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGa                                                             >  2:4386997/1‑93 (MQ=255)
                                 cGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAac                                                           <  2:3629446/93‑1 (MQ=255)
                                  ggTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacc                                                          >  1:1963206/1‑93 (MQ=255)
                                   gTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacca                                                         <  2:269191/93‑1 (MQ=255)
                                   gTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacca                                                         >  2:1209316/1‑93 (MQ=255)
                                   gTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacca                                                         <  2:1146566/93‑1 (MQ=255)
                                   gTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacca                                                         <  1:6089149/93‑1 (MQ=255)
                                   gTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacca                                                         <  1:5491649/93‑1 (MQ=255)
                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                        >  2:3811179/1‑93 (MQ=255)
                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                        <  1:3628769/93‑1 (MQ=255)
                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                        <  2:2153938/93‑1 (MQ=255)
                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                        <  1:7284751/93‑1 (MQ=255)
                                     cAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccacc                                                       <  1:380815/93‑1 (MQ=255)
                                     cAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccacc                                                       <  1:4146181/93‑1 (MQ=255)
                                      aGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCg                                                      >  1:5602770/1‑93 (MQ=255)
                                       gTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGc                                                     >  1:308671/1‑93 (MQ=255)
                                       gTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGc                                                     >  2:6643242/1‑93 (MQ=255)
                                        tACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCa                                                    <  2:5356661/93‑1 (MQ=255)
                                            gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                                <  2:3094458/93‑1 (MQ=255)
                                               gtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGa                                             >  1:2749537/1‑93 (MQ=255)
                                                taCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAc                                            >  2:696605/1‑93 (MQ=255)
                                                     ccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAc                                       >  2:1749255/1‑93 (MQ=255)
                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                      >  2:729620/1‑93 (MQ=255)
                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                      >  1:2966143/1‑93 (MQ=255)
                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                      <  1:3314790/93‑1 (MQ=255)
                                                        ttAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTc                                    >  1:3243034/1‑93 (MQ=255)
                                                           gCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGt                                 >  1:6895781/1‑93 (MQ=255)
                                                           gCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGt                                 >  1:2463211/1‑93 (MQ=255)
                                                            cAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGtt                                <  1:5513875/93‑1 (MQ=255)
                                                              aTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCg                              >  1:7427636/1‑93 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                             <  2:5074242/93‑1 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                             <  1:1401360/93‑1 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                             >  2:4611146/1‑93 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                             <  2:344716/93‑1 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                             >  2:575225/1‑93 (MQ=255)
                                                               tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTACAGACCAAACTTCAGTTCGc                             <  2:6934246/93‑1 (MQ=255)
                                                                 aCGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTg                           <  1:1038104/93‑1 (MQ=255)
                                                                 aCGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTg                           <  1:3394825/93‑1 (MQ=255)
                                                                 aCGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTg                           <  1:424999/93‑1 (MQ=255)
                                                                    tAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGt                        >  1:2610744/1‑93 (MQ=255)
                                                                    tAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGt                        <  2:2502200/93‑1 (MQ=255)
                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                       <  2:5005458/93‑1 (MQ=255)
                                                                      gTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGttt                      >  1:6116657/1‑93 (MQ=255)
                                                                       tCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTg                     >  2:4045142/1‑93 (MQ=255)
                                                                       tCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTg                     >  2:529959/1‑93 (MQ=255)
                                                                         agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                   <  1:154936/93‑1 (MQ=255)
                                                                         agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                   <  1:1110680/93‑1 (MQ=255)
                                                                         agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                   >  2:4095949/1‑93 (MQ=255)
                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt                  <  2:7491663/93‑1 (MQ=255)
                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt                  <  2:379046/93‑1 (MQ=255)
                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt                  >  1:789884/1‑93 (MQ=255)
                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt                  >  1:5233241/1‑93 (MQ=255)
                                                                           agaAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTg                 <  1:1181667/93‑1 (MQ=255)
                                                                           agaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTg                 <  2:7398014/93‑1 (MQ=255)
                                                                           agaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTg                 <  1:6776387/93‑1 (MQ=255)
                                                                            gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg                <  1:318776/93‑1 (MQ=255)
                                                                             aaaCGCCCCAGCACCGGGTTAGCAGTAATTCCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGc               <  2:4686081/93‑1 (MQ=255)
                                                                                 gCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcgc           <  1:3557642/93‑1 (MQ=255)
                                                                                    ccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGtt        >  2:2842137/1‑93 (MQ=255)
                                                                                    ccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGtt        >  1:6474175/1‑93 (MQ=255)
                                                                                     cAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGttt       >  1:2099797/1‑93 (MQ=255)
                                                                                      aGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTa      <  2:6838102/93‑1 (MQ=255)
                                                                                      aGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTa      <  2:3339252/93‑1 (MQ=255)
                                                                                       gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt     <  2:5696945/93‑1 (MQ=255)
                                                                                       gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt     <  1:3163013/93‑1 (MQ=255)
                                                                                         aTCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCg   <  1:4463162/93‑1 (MQ=255)
                                                                                          |                                                                                            
GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT  >  NC_000913/3240033‑3240215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216
                                                                                             |                                                                                             
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCAC                                                                                               >  SRR4302542.5718965/1‑94 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302542.997390/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              >  SRR4302542.5514704/1‑94 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              >  SRR4302542.2909340/1‑94 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302542.1237696/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              >  SRR4302542.6801583/1‑94 (MQ=60)
  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCG                                                                                             >  SRR4302542.1605982/1‑94 (MQ=60)
   GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGG                                                                                            >  SRR4302542.6381629/1‑94 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGG                                                                                           >  SRR4302542.6756060/1‑94 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           >  SRR4302542.1206705/1‑94 (MQ=60)
     AACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGT                                                                                          <  SRR4302542.5911174/94‑1 (MQ=60)
     AACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGT                                                                                          >  SRR4302542.1267948/1‑94 (MQ=60)
       CTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTA                                                                                        >  SRR4302542.7393755/1‑94 (MQ=60)
       CTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTA                                                                                        <  SRR4302542.6616369/94‑1 (MQ=60)
        TGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAG                                                                                       <  SRR4302542.5903060/94‑1 (MQ=60)
        TGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAG                                                                                       >  SRR4302542.4973781/1‑94 (MQ=60)
           caggcaaactcagcgaactgaagtttGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAG                                                                                    <  SRR4302542.4455857/68‑1 (MQ=60)
           TCTGCGCCAAACATCTCCGGCCCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTGGCAG                                                                                    <  SRR4302542.7572688/94‑1 (MQ=60)
           TCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAG                                                                                    <  SRR4302542.1312269/94‑1 (MQ=60)
            CTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGT                                                                                   >  SRR4302542.7136209/1‑94 (MQ=60)
            CTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGT                                                                                   <  SRR4302542.4544658/94‑1 (MQ=60)
            CTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGT                                                                                   >  SRR4302542.2472991/1‑94 (MQ=60)
            CTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGT                                                                                   >  SRR4302542.1764844/1‑94 (MQ=60)
              GCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAA                                                                                 >  SRR4302542.315347/1‑94 (MQ=60)
               CGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAAT                                                                                <  SRR4302542.6263772/94‑1 (MQ=60)
                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               >  SRR4302542.3214332/1‑94 (MQ=60)
                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               <  SRR4302542.2274430/94‑1 (MQ=60)
                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               >  SRR4302542.327961/1‑94 (MQ=60)
                 CCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAC                                                                              >  SRR4302542.4348364/1‑94 (MQ=60)
                 CCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAC                                                                              >  SRR4302542.3589788/1‑94 (MQ=60)
                 CCAAACATCTCCGGCACTACGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATAC                                                                              <  SRR4302542.5070149/94‑1 (MQ=60)
                  CAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACC                                                                             >  SRR4302542.5856143/1‑94 (MQ=60)
                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCA                                                                            >  SRR4302542.3999162/1‑94 (MQ=60)
                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCA                                                                            >  SRR4302542.481131/1‑94 (MQ=60)
                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCA                                                                            >  SRR4302542.7494999/1‑94 (MQ=60)
                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          <  SRR4302542.4343281/94‑1 (MQ=60)
                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          >  SRR4302542.1806883/1‑94 (MQ=60)
                      CATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAA                                                                         <  SRR4302542.5307157/94‑1 (MQ=60)
                       ATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAA                                                                        >  SRR4302542.598806/1‑94 (MQ=60)
                       ATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAA                                                                        >  SRR4302542.1521314/1‑94 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302542.5628042/94‑1 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302542.1363052/94‑1 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302542.1339159/94‑1 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302542.5846551/94‑1 (MQ=60)
                         CTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGA                                                                      >  SRR4302542.4693577/1‑94 (MQ=60)
                         CTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGA                                                                      >  SRR4302542.6352742/1‑94 (MQ=60)
                         CTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGA                                                                      >  SRR4302542.1861851/1‑94 (MQ=60)
                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACC                                                                    <  SRR4302542.4352925/94‑1 (MQ=60)
                            CGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCG                                                                   >  SRR4302542.451082/1‑94 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               <  SRR4302542.6063517/94‑1 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302542.2671453/1‑94 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302542.1603188/1‑94 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302542.7294743/1‑94 (MQ=60)
                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302542.4456636/1‑94 (MQ=60)
                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302542.801835/1‑94 (MQ=60)
                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302542.4552546/1‑94 (MQ=60)
                                   TCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAAC                                                            <  SRR4302542.1131555/94‑1 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACC                                                           <  SRR4302542.3618316/94‑1 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACC                                                           >  SRR4302542.1980177/1‑94 (MQ=60)
                                     GGCCAGTACGTTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCA                                                          <  SRR4302542.4680911/94‑1 (MQ=60)
                                      GTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAC                                                         <  SRR4302542.6148106/94‑1 (MQ=60)
                                      GTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAC                                                         >  SRR4302542.5651114/1‑94 (MQ=60)
                                      GTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAC                                                         <  SRR4302542.5544992/94‑1 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        >  SRR4302542.5167463/1‑94 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        <  SRR4302542.3662578/94‑1 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        <  SRR4302542.7356177/94‑1 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        <  SRR4302542.6442336/94‑1 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        <  SRR4302542.4274637/94‑1 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302542.7128393/94‑1 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302542.384126/94‑1 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302542.4187027/94‑1 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       >  SRR4302542.5657200/1‑94 (MQ=60)
                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      >  SRR4302542.311363/1‑94 (MQ=60)
                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  >  SRR4302542.6620094/1‑94 (MQ=60)
                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  >  SRR4302542.3193986/1‑94 (MQ=60)
                                              GGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCC                                                 <  SRR4302542.5926744/94‑1 (MQ=60)
                                              GGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCC                                                 <  SRR4302542.4688773/94‑1 (MQ=60)
                                                TAGTTCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCTCCCCAG                                               <  SRR4302542.925086/94‑1 (MQ=60)
                                                TAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAG                                               <  SRR4302542.3616353/94‑1 (MQ=60)
                                                 AGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGA                                              >  SRR4302542.2776042/1‑94 (MQ=60)
                                                  GTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAC                                             >  SRR4302542.299586/1‑94 (MQ=60)
                                                     CCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAA                                          <  SRR4302542.357137/94‑1 (MQ=60)
                                                       GCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAC                                        <  SRR4302542.2075452/94‑1 (MQ=60)
                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       >  SRR4302542.2994560/1‑94 (MQ=60)
                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       <  SRR4302542.579754/94‑1 (MQ=60)
                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       >  SRR4302542.3000921/1‑94 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      <  SRR4302542.5898527/94‑1 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      <  SRR4302542.3346030/94‑1 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302542.3864842/1‑94 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302542.1289843/1‑94 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302542.3752053/1‑94 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302542.3273689/1‑94 (MQ=60)
                                                           TTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCA                                    >  SRR4302542.6806485/1‑94 (MQ=60)
                                                             AGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGT                                  <  SRR4302542.6089538/94‑1 (MQ=60)
                                                             AGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGT                                  >  SRR4302542.6963499/1‑94 (MQ=60)
                                                             AGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGT                                  >  SRR4302542.2487132/1‑94 (MQ=60)
                                                             AGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGT                                  >  SRR4302542.5331169/1‑94 (MQ=60)
                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302542.6155894/94‑1 (MQ=60)
                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302542.6170180/94‑1 (MQ=60)
                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302542.1918977/94‑1 (MQ=60)
                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                <  SRR4302542.5567459/94‑1 (MQ=60)
                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                >  SRR4302542.3318585/1‑94 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               >  SRR4302542.5564292/1‑94 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               >  SRR4302542.7500322/1‑94 (MQ=60)
                                                                  TCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCT                             <  SRR4302542.6925966/94‑1 (MQ=60)
                                                                  TCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCT                             <  SRR4302542.1413476/94‑1 (MQ=60)
                                                                    ACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGA                           <  SRR4302542.1047023/94‑1 (MQ=60)
                                                                    ACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGA                           <  SRR4302542.428640/94‑1 (MQ=60)
                                                                    ACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGA                           <  SRR4302542.3426676/94‑1 (MQ=60)
                                                                     CGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAG                          <  SRR4302542.1799449/94‑1 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         <  SRR4302542.1116863/94‑1 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         >  SRR4302542.2635982/1‑94 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         >  SRR4302542.3356385/1‑94 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       >  SRR4302542.6175849/1‑94 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302542.2181207/94‑1 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302542.2817542/94‑1 (MQ=60)
                                                                          TCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGC                     <  SRR4302542.4243321/94‑1 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302542.156245/94‑1 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302542.3248392/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302542.1984810/94‑1 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302542.5284125/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302542.796691/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302542.1120183/94‑1 (MQ=60)
                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302542.2620410/94‑1 (MQ=60)
                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302542.2957102/94‑1 (MQ=60)
                                                                              AGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 <  SRR4302542.1191866/94‑1 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 <  SRR4302542.6843049/94‑1 (MQ=60)
                                                                               GAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGC                <  SRR4302542.321545/94‑1 (MQ=60)
                                                                                 AACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTC              >  SRR4302542.2345901/1‑94 (MQ=60)
                                                                                  ACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCG             <  SRR4302542.1461561/94‑1 (MQ=60)
                                                                                   CGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGC            >  SRR4302542.3244484/1‑94 (MQ=60)
                                                                                   CGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGC            >  SRR4302542.1246453/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           <  SRR4302542.3590876/94‑1 (MQ=60)
                                                                                      CCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         <  SRR4302542.2493409/94‑1 (MQ=60)
                                                                                      CCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         >  SRR4302542.1169801/1‑94 (MQ=60)
                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         >  SRR4302542.6537959/1‑94 (MQ=60)
                                                                                       CCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        <  SRR4302542.6887711/94‑1 (MQ=60)
                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        >  SRR4302542.2117919/1‑94 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      >  SRR4302542.768592/1‑94 (MQ=60)
                                                                                          GCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATC     <  SRR4302542.7027239/94‑1 (MQ=60)
                                                                                          GCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATC     <  SRR4302542.3193030/94‑1 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    <  SRR4302542.6810858/94‑1 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    <  SRR4302542.7076203/94‑1 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    <  SRR4302542.3516049/94‑1 (MQ=60)
                                                                                            ATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT   <  SRR4302542.4506943/94‑1 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  SRR4302542.6723838/1‑94 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302542.4794193/94‑1 (MQ=60)
                                                                                             |                                                                                             
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑