Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,838 +G coding (2222/2241 nt) tdcE ← putative formate acetyltransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,8371.G100.0% 849.0 / NA 250A741A (GCG→GCC) tdcEputative formate acetyltransferase 3
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (118/132);  total (118/132)

Only 100 of 267 total aligned reads displayed.

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924
                                                                                             |                                                                                       
tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          >  1:3616156/1‑93 (MQ=255)
 ttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g                                                                                        >  2:2967149/1‑93 (MQ=255)
  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                       <  2:680799/93‑2 (MQ=255)
  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                       >  2:5505452/1‑92 (MQ=255)
    tCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg                                                                                     <  1:1848604/93‑4 (MQ=255)
    tCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg                                                                                     >  2:6634150/1‑90 (MQ=255)
    tCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg                                                                                     >  1:3328326/1‑90 (MQ=255)
       tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAg                                                                                   >  2:4827751/1‑93 (MQ=255)
       tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAg                                                                                   >  2:5416430/1‑93 (MQ=255)
       tACATACTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAg                                                                                   >  1:1963681/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  <  1:6485981/93‑1 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  2:1849278/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  1:6859664/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  <  2:1010369/93‑1 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  2:6165893/1‑93 (MQ=255)
          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                >  2:1890202/1‑93 (MQ=255)
           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               >  2:4125032/1‑93 (MQ=255)
             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  1:4246109/1‑93 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            >  2:210666/1‑93 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            <  1:4276212/93‑1 (MQ=255)
               ccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                           <  1:2696658/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAATGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  2:7419294/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  2:2060210/93‑1 (MQ=255)
                 ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                                         >  1:5439207/1‑93 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                        <  1:3913287/93‑1 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                        >  1:6781867/1‑93 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                      <  2:5931986/93‑1 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                      <  2:4599206/93‑1 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                      <  2:4029000/93‑1 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                     >  1:4617832/1‑93 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCCGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                     >  2:138471/1‑93 (MQ=255)
                      cAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAtt                                                                    <  2:3260421/93‑1 (MQ=255)
                       agagCGCCTTGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   >  1:4535252/1‑93 (MQ=255)
                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                <  1:1415620/93‑1 (MQ=255)
                           ggcCTGGGTAAAGGTACGTGAGATAACTTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGTCACGGATTGTCAg                                                               <  1:7530808/92‑1 (MQ=255)
                           cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                               <  1:5038963/93‑1 (MQ=255)
                             ccTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                             >  2:6550403/1‑93 (MQ=255)
                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTa                                                           >  2:4364343/1‑93 (MQ=255)
                                gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAg                                                          <  1:5198602/93‑1 (MQ=255)
                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                        >  1:7174662/1‑93 (MQ=255)
                                      aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt                                                    >  2:7082999/1‑93 (MQ=255)
                                        gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                  <  1:7218558/93‑1 (MQ=255)
                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                 >  2:3474979/1‑93 (MQ=255)
                                          aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                                <  1:2593001/93‑1 (MQ=255)
                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                               <  2:1185950/93‑1 (MQ=255)
                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                              <  2:2898717/93‑1 (MQ=255)
                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                             >  1:6061075/1‑93 (MQ=255)
                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                             <  1:1738684/93‑1 (MQ=255)
                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                             >  1:6411973/1‑93 (MQ=255)
                                               aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt                                           <  1:2312405/93‑1 (MQ=255)
                                               aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGCCAGGTTAGGATATTTTTCCGGGt                                           <  1:3212831/93‑1 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        >  1:3399950/1‑93 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        >  2:6867107/1‑93 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        >  1:5354961/1‑93 (MQ=255)
                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                       <  1:3667845/93‑1 (MQ=255)
                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCg                                      >  1:5775777/1‑93 (MQ=255)
                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     >  2:7320014/1‑93 (MQ=255)
                                                      aTCCTGTTGCTGTTTACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAt                                    >  2:6450899/1‑93 (MQ=255)
                                                      aTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAt                                    <  1:953420/93‑1 (MQ=255)
                                                      aTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAt                                    >  1:5950633/1‑93 (MQ=255)
                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                   >  2:1496120/1‑93 (MQ=255)
                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                   >  1:6998998/1‑93 (MQ=255)
                                                        ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATgg                                  <  1:5611144/93‑1 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 >  1:1059480/1‑93 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 >  2:3046797/1‑93 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 >  2:2483271/1‑93 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 >  1:1094154/1‑93 (MQ=255)
                                                          tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCa                                >  2:4913472/1‑93 (MQ=255)
                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                            <  2:2753942/93‑1 (MQ=255)
                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                            <  1:1596506/93‑1 (MQ=255)
                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                            <  1:6459493/93‑1 (MQ=255)
                                                                 gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                         <  2:987777/93‑1 (MQ=255)
                                                                  ttCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagcag                        <  1:6052865/93‑1 (MQ=255)
                                                                   tCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCagcagc                       <  2:3856082/93‑1 (MQ=255)
                                                                   tCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCagcagc                       <  1:3753901/93‑1 (MQ=255)
                                                                    cACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca                      <  2:5606841/93‑1 (MQ=255)
                                                                        ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                  >  1:1014156/1‑93 (MQ=255)
                                                                        ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                  <  1:2411776/93‑1 (MQ=255)
                                                                        ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                  >  1:4866125/1‑93 (MQ=255)
                                                                        ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                  <  2:3721174/93‑1 (MQ=255)
                                                                         gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                 <  2:349388/93‑1 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc                <  2:1997713/93‑1 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc                >  2:6764100/1‑93 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc                <  1:3507551/93‑1 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc                <  2:3663744/93‑1 (MQ=255)
                                                                            aGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGa              <  2:5345763/93‑1 (MQ=255)
                                                                             gTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt             <  1:434799/93‑1 (MQ=255)
                                                                             gTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt             >  1:2289565/1‑93 (MQ=255)
                                                                              tGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt            >  2:5655289/1‑93 (MQ=255)
                                                                                cGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCa          <  2:7293601/93‑1 (MQ=255)
                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         >  1:3814795/1‑93 (MQ=255)
                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         <  1:5117071/93‑1 (MQ=255)
                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         >  2:1662611/1‑93 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        >  1:4169082/1‑93 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        >  2:4677040/1‑93 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        <  1:1213940/93‑1 (MQ=255)
                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:2267365/93‑1 (MQ=255)
                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:5847768/93‑1 (MQ=255)
                                                                                    gAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTCTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTac      <  1:5843174/93‑1 (MQ=255)
                                                                                       gcgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgtt   <  1:6339929/93‑1 (MQ=255)
                                                                                             |                                                                                       
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  >  NC_000913/3257745‑3257930
                                                                                              |                                                                                            
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G                                                                                              >  SRR4302542.647985/1‑94 (MQ=60)
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G                                                                                              <  SRR4302542.169682/94‑1 (MQ=60)
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G                                                                                              <  SRR4302542.4303486/94‑1 (MQ=60)
   TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggcg                                                                                           >  SRR4302542.4983381/1‑91 (MQ=60)
   TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggcg                                                                                           <  SRR4302542.7275072/94‑4 (MQ=60)
   TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggcg                                                                                           >  SRR4302542.3359654/1‑91 (MQ=60)
   TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggcg                                                                                           <  SRR4302542.7399100/94‑4 (MQ=60)
    TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGT                                                                                           <  SRR4302542.1864597/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTTCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         <  SRR4302542.3684241/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         <  SRR4302542.1977506/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         >  SRR4302542.1163467/1‑94 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         <  SRR4302542.6485169/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         <  SRR4302542.3954935/94‑1 (MQ=60)
      ATACATACTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                         >  SRR4302542.1980653/1‑94 (MQ=60)
       TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                        >  SRR4302542.6927067/1‑94 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCC                                                                                       <  SRR4302542.6549893/94‑1 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                     <  SRR4302542.6996233/94‑1 (MQ=60)
            CCTCCGGCGTCAGAGCGCGTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                   <  SRR4302542.2548033/94‑1 (MQ=60)
            CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                   >  SRR4302542.4287944/1‑94 (MQ=60)
            CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                   <  SRR4302542.2872967/94‑1 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                  >  SRR4302542.6017863/1‑94 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                  >  SRR4302542.4972832/1‑94 (MQ=60)
              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGAC                                                                                 <  SRR4302542.4318278/94‑1 (MQ=60)
               CCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACA                                                                                <  SRR4302542.2722665/94‑1 (MQ=60)
                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                               >  SRR4302542.5492053/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACG                                                                              >  SRR4302542.6384868/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACG                                                                              <  SRR4302542.1777731/94‑1 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACG                                                                              >  SRR4302542.6848577/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACG                                                                              >  SRR4302542.1083824/1‑94 (MQ=60)
                  GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                             <  SRR4302542.3951905/94‑1 (MQ=60)
                  GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                             >  SRR4302542.5701070/1‑94 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGA                                                                            >  SRR4302542.4455528/1‑94 (MQ=60)
                    GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                           >  SRR4302542.4662942/1‑94 (MQ=60)
                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATT                                                                          <  SRR4302542.5183444/94‑1 (MQ=60)
                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATT                                                                          <  SRR4302542.1583640/94‑1 (MQ=60)
                      CAGAGCGCCTTGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTG                                                                         >  SRR4302542.4579665/1‑94 (MQ=60)
                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTG                                                                         <  SRR4302542.1918566/94‑1 (MQ=60)
                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                     <  SRR4302542.5190650/94‑1 (MQ=60)
                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                     <  SRR4302542.1427883/94‑1 (MQ=60)
                           GGCCTGGGTAAAGGTACGTGAGATAACTTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGTCACGGATTGTCAGG                                                                    <  SRR4302542.7604840/94‑1 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                    <  SRR4302542.5087880/94‑1 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                    >  SRR4302542.5398391/1‑94 (MQ=60)
                            GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                   <  SRR4302542.1683617/94‑1 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                  >  SRR4302542.6942909/1‑94 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTA                                                                 <  SRR4302542.3801052/94‑1 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTA                                                                 >  SRR4302542.2336840/1‑94 (MQ=60)
                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                               <  SRR4302542.2164566/94‑1 (MQ=60)
                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                               <  SRR4302542.5249118/94‑1 (MQ=60)
                                 GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                              >  SRR4302542.7245041/1‑94 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                            >  SRR4302542.186922/1‑94 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                            <  SRR4302542.610675/94‑1 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                            <  SRR4302542.3532299/94‑1 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                            <  SRR4302542.5984782/94‑1 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           <  SRR4302542.6404571/94‑1 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                           <  SRR4302542.3534513/94‑1 (MQ=60)
                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                          >  SRR4302542.3283310/1‑94 (MQ=60)
                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                          >  SRR4302542.5424985/1‑94 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       >  SRR4302542.7481087/1‑94 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                       <  SRR4302542.7289369/94‑1 (MQ=60)
                                         TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                      >  SRR4302542.2319354/1‑94 (MQ=60)
                                         TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                      >  SRR4302542.4265129/1‑94 (MQ=60)
                                         TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                      >  SRR4302542.6664718/1‑94 (MQ=60)
                                          ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                     <  SRR4302542.2618113/94‑1 (MQ=60)
                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                   >  SRR4302542.6119743/1‑94 (MQ=60)
                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                   >  SRR4302542.6475157/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                  <  SRR4302542.4231860/94‑1 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                  <  SRR4302542.1753768/94‑1 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                 >  SRR4302542.3835563/1‑94 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                 <  SRR4302542.266012/94‑1 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                <  SRR4302542.2332258/94‑1 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                <  SRR4302542.4387017/94‑1 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGCCAGGTTAGGATATTTTTCCGGGTG                                                <  SRR4302542.3243242/94‑1 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                              >  SRR4302542.3431838/1‑94 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                              >  SRR4302542.5407003/1‑94 (MQ=60)
                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                            >  SRR4302542.5831844/1‑94 (MQ=60)
                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                            <  SRR4302542.3702884/94‑1 (MQ=60)
                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                          >  SRR4302542.6008264/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302542.7067750/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         <  SRR4302542.6347571/94‑1 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302542.5139021/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         <  SRR4302542.961606/94‑1 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         <  SRR4302542.3304344/94‑1 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                         >  SRR4302542.3876068/1‑94 (MQ=60)
                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                        <  SRR4302542.4516594/94‑1 (MQ=60)
                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                        <  SRR4302542.4320028/94‑1 (MQ=60)
                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                        <  SRR4302542.4169080/94‑1 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       <  SRR4302542.5665660/94‑1 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       >  SRR4302542.1068586/1‑94 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       >  SRR4302542.1103527/1‑94 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       <  SRR4302542.6431663/94‑1 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       >  SRR4302542.2014270/1‑94 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                       >  SRR4302542.6536301/1‑94 (MQ=60)
                                                        CCTGTTGCTCTTCACGGTTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGTGgtacttgaa                                       >  SRR4302542.6497554/1‑85 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                      <  SRR4302542.5868841/94‑1 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                      >  SRR4302542.659366/1‑94 (MQ=60)
                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                     >  SRR4302542.2431473/1‑94 (MQ=60)
                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                     <  SRR4302542.1066384/94‑1 (MQ=60)
                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                    >  SRR4302542.2561077/1‑94 (MQ=60)
                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                    >  SRR4302542.3408983/1‑94 (MQ=60)
                                                            TTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                   >  SRR4302542.6016052/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                  <  SRR4302542.7225078/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                 <  SRR4302542.6523097/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                 <  SRR4302542.6523096/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                 <  SRR4302542.1610296/94‑1 (MQ=60)
                                                               CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                >  SRR4302542.1007114/1‑94 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGATAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                               <  SRR4302542.4759425/94‑1 (MQ=60)
                                                                 GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAG                              <  SRR4302542.553760/94‑1 (MQ=60)
                                                                  TTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                             <  SRR4302542.6111447/94‑1 (MQ=60)
                                                                   TCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                            <  SRR4302542.5998739/94‑1 (MQ=60)
                                                                   TCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                            <  SRR4302542.3791040/94‑1 (MQ=60)
                                                                   TCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                            <  SRR4302542.5669033/94‑1 (MQ=60)
                                                                     ACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                          >  SRR4302542.4707377/1‑94 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                         <  SRR4302542.1502785/94‑1 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                        >  SRR4302542.3965279/1‑94 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                        >  SRR4302542.4913502/1‑94 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                        >  SRR4302542.1022894/1‑94 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTC                        >  SRR4302542.3691897/1‑94 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                       <  SRR4302542.1100585/94‑1 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                       <  SRR4302542.5306560/94‑1 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                       <  SRR4302542.2435223/94‑1 (MQ=60)
                                                                         GTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                      >  SRR4302542.6914537/1‑94 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                     <  SRR4302542.3540351/94‑1 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                     <  SRR4302542.3630570/94‑1 (MQ=60)
                                                                            AGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                   >  SRR4302542.2309237/1‑94 (MQ=60)
                                                                             GTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATT                  <  SRR4302542.438560/94‑1 (MQ=60)
                                                                                CGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT               >  SRR4302542.30831/1‑94 (MQ=60)
                                                                                CGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT               >  SRR4302542.5571964/1‑94 (MQ=60)
                                                                                CGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT               >  SRR4302542.3852531/1‑94 (MQ=60)
                                                                                 GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              <  SRR4302542.7125465/94‑1 (MQ=60)
                                                                                 GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              <  SRR4302542.1128447/94‑1 (MQ=60)
                                                                                 GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              >  SRR4302542.4210201/1‑94 (MQ=60)
                                                                                 GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              <  SRR4302542.5166765/94‑1 (MQ=60)
                                                                                 GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT              <  SRR4302542.3776737/94‑1 (MQ=60)
                                                                                  TTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA             <  SRR4302542.1224384/94‑1 (MQ=60)
                                                                                  TTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA             >  SRR4302542.3577530/1‑94 (MQ=60)
                                                                                   TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC            <  SRR4302542.829070/94‑1 (MQ=60)
                                                                                    GAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG           <  SRR4302542.3788979/94‑1 (MQ=60)
                                                                                    GAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG           <  SRR4302542.856755/94‑1 (MQ=60)
                                                                                    GAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTCTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG           <  SRR4302542.5899847/94‑1 (MQ=60)
                                                                                       GCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG        <  SRR4302542.1421209/94‑1 (MQ=60)
                                                                                       GCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG        <  SRR4302542.6402386/94‑1 (MQ=60)
                                                                                        CGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA       <  SRR4302542.4646385/94‑1 (MQ=60)
                                                                                         GCACGGCGTAGCCAGAGACACGGATTGTCAGGTAAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC      <  SRR4302542.3606567/94‑1 (MQ=60)
                                                                                          CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG     >  SRR4302542.175693/1‑94 (MQ=60)
                                                                                            cgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTCTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT   <  SRR4302542.4650636/92‑1 (MQ=60)
                                                                                            G‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  <  SRR4302542.3247814/94‑1 (MQ=60)
                                                                                            G‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  >  SRR4302542.3474835/1‑94 (MQ=60)
                                                                                              |                                                                                            
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG  >  NC_000913/3257745‑3257930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑