Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,236	G→C	A59G (GCT→GGT)	b3124 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,236	0	G	C	84.3%	702.2 / 110.9	249	A59G (GCT→GGT)	b3124	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (15/24); new base C (99/111); total (114/135)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01

Only 100 of 255 total aligned reads displayed.

TTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCC  >  NC_000913/3269145‑3269328

 tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGcc                                                                                            <  2:3785257/93‑1 (MQ=255)

   aaTAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACcccc                                                                                          <  2:7275269/93‑1 (MQ=255)

    aTAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAcccccc                                                                                         >  1:2450919/1‑93 (MQ=255)

    aTAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGccccc                                                                                         <  1:5203765/93‑1 (MQ=255)

    aTAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCAcccccc                                                                                         <  2:7113956/93‑1 (MQ=255)

      aaaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTg                                                                                       <  2:2015386/93‑1 (MQ=255)

      aaaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTg                                                                                       >  2:3466223/1‑93 (MQ=255)

       aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTgg                                                                                      <  1:7215464/93‑1 (MQ=255)

       aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTgg                                                                                      <  1:5651888/93‑1 (MQ=255)

        aCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTggg                                                                                     >  2:4045423/1‑93 (MQ=255)

        aCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTggg                                                                                     <  1:3786005/93‑1 (MQ=255)

         cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGt                                                                                    <  1:2580405/93‑1 (MQ=255)

          gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTg                                                                                   >  1:1281049/1‑93 (MQ=255)

          gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTg                                                                                   >  2:7142165/1‑93 (MQ=255)

                ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCa                                                                             >  1:7364330/1‑93 (MQ=255)

                 tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCaa                                                                            >  2:2799303/1‑93 (MQ=255)

                   ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatc                                                                          >  2:6316451/1‑93 (MQ=255)

                   ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTCCAatc                                                                          <  1:4702742/93‑1 (MQ=255)

                    cATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatca                                                                         <  1:6035960/93‑1 (MQ=255)

                     aTCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatcat                                                                        <  2:2406812/93‑1 (MQ=255)

                      tCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAatcatc                                                                       >  2:1588616/1‑93 (MQ=255)

                      tCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatcatc                                                                       >  1:3939087/1‑93 (MQ=255)

                       cGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCg                                                                      <  2:3189202/93‑1 (MQ=255)

                        gCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGc                                                                     >  1:4465826/1‑93 (MQ=255)

                          cGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCtt                                                                   <  2:2300016/93‑1 (MQ=255)

                           ggAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTc                                                                  >  2:5024010/1‑93 (MQ=255)

                            gagaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCGCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTcc                                                                 >  1:6221423/1‑93 (MQ=255)

                            gagaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTcc                                                                 <  2:750334/93‑1 (MQ=255)

                            gagaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTcc                                                                 >  1:3289290/1‑93 (MQ=255)

                             agaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCa                                                                <  2:2248376/93‑1 (MQ=255)

                              gaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAc                                                               <  2:390969/93‑1 (MQ=255)

                              gaTCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAc                                                               >  2:45484/1‑93 (MQ=255)

                                tCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCg                                                             <  1:5496211/93‑1 (MQ=255)

                                   cccAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTc                                                          >  1:4934003/1‑93 (MQ=255)

                                   cccAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTc                                                          >  1:2210956/1‑93 (MQ=255)

                                    ccAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTcc                                                         >  1:1607135/1‑93 (MQ=255)

                                     cAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCt                                                        >  2:5365684/1‑93 (MQ=255)

                                      aaCTGGCATTCACTTTCTCGGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCt                                                        >  1:6158151/1‑93 (MQ=255)

                                      aaCTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCtt                                                       >  2:1294168/1‑93 (MQ=255)

                                       aCTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTc                                                      >  2:2897060/1‑93 (MQ=255)

                                       aCTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTc                                                      <  2:3305172/93‑1 (MQ=255)

                                         tGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                    <  2:5805224/93‑1 (MQ=255)

                                         tGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                    >  2:3682877/1‑93 (MQ=255)

                                         tGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                    <  1:3570321/93‑1 (MQ=255)

                                            cATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAc                                                 >  1:5069385/1‑93 (MQ=255)

                                             aTTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAcc                                                >  1:4755135/1‑93 (MQ=255)

                                              ttCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCg                                               >  2:6788231/1‑93 (MQ=255)

                                              ttCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCg                                               >  1:3313392/1‑93 (MQ=255)

                                              ttCAATTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCg                                               <  1:5728163/93‑1 (MQ=255)

                                               tCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGt                                              <  2:1757005/93‑1 (MQ=255)

                                                 aCTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCg                                            <  2:6869034/93‑1 (MQ=255)

                                                 aCTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCg                                            >  2:5147649/1‑93 (MQ=255)

                                                   tttCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGc                                          >  2:2330448/1‑93 (MQ=255)

                                                     tctc‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCaa                                        >  2:3639923/1‑93 (MQ=255)

                                                     tctc‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCaa                                        >  2:5447391/1‑93 (MQ=255)

                                                        c‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCg                                     <  1:2662927/93‑1 (MQ=255)

                                                        c‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCg                                     <  2:2162216/93‑1 (MQ=255)

                                                          gCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCgg                                    >  2:4366111/1‑93 (MQ=255)

                                                          gCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCgg                                    >  1:3728735/1‑93 (MQ=255)

                                                          gCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCgg                                    <  1:4510377/93‑1 (MQ=255)

                                                            ccAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa                                  <  1:3498519/93‑1 (MQ=255)

                                                             cAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAc                                 <  1:4282836/93‑1 (MQ=255)

                                                             cAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAc                                 <  2:448442/93‑1 (MQ=255)

                                                             cAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAc                                 <  1:1580561/93‑1 (MQ=255)

                                                              aGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACa                                <  2:914211/93‑1 (MQ=255)

                                                               gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                               >  1:6869149/1‑93 (MQ=255)

                                                               gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                               >  2:6125155/1‑93 (MQ=255)

                                                                  gCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                            >  1:6503147/1‑93 (MQ=255)

                                                                  gCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                            <  2:5693557/93‑1 (MQ=255)

                                                                  gCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                            <  1:7129771/93‑1 (MQ=255)

                                                                  gCCCTGAAACCCAGGCGTGACGTTCTCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                            <  1:6827128/93‑1 (MQ=255)

                                                                   cccTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAc                           >  2:2547301/1‑93 (MQ=255)

                                                                     cTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                         <  1:209543/93‑1 (MQ=255)

                                                                     cTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                         >  1:4218836/1‑93 (MQ=255)

                                                                     cTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                         <  2:4156424/93‑1 (MQ=255)

                                                                     cTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                         >  1:2019370/1‑93 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        <  1:4098851/93‑1 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        <  2:4852513/93‑1 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        >  2:5091876/1‑93 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        <  2:5563172/93‑1 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        >  1:2831799/1‑93 (MQ=255)

                                                                      tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                        >  2:7316105/1‑93 (MQ=255)

                                                                       gTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAc                       <  2:2634339/93‑1 (MQ=255)

                                                                       gTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAc                       <  2:6543799/93‑1 (MQ=255)

                                                                         aaCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtg                     >  2:3118180/1‑93 (MQ=255)

                                                                           cccAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgtg                   >  2:6285338/1‑93 (MQ=255)

                                                                             cAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCa                 <  1:6884103/93‑1 (MQ=255)

                                                                             cAGGCGTGACGTTCACCCCCCCGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGCCGGCAACCGGAACAGAAACGTACTGTGCa                 >  1:1761145/1‑93 (MQ=255)

                                                                              aGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAt                <  2:1856788/93‑1 (MQ=255)

                                                                                gCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCa              <  1:806490/93‑1 (MQ=255)

                                                                                 cGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAg             <  1:2979793/93‑1 (MQ=255)

                                                                                    gACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaa          >  2:7401841/1‑93 (MQ=255)

                                                                                     aCGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaa         <  1:342017/93‑1 (MQ=255)

                                                                                      cGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaa        <  2:1060110/93‑1 (MQ=255)

                                                                                       gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa       <  2:601426/93‑1 (MQ=255)

                                                                                       gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa       <  1:953116/93‑1 (MQ=255)

                                                                                       gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa       >  1:1123740/1‑93 (MQ=255)

                                                                                        ttCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAt      >  2:4610062/1‑93 (MQ=255)

                                                                                         tCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt     <  1:5446732/93‑1 (MQ=255)

                                                                                            gCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTcc  >  2:5852664/1‑93 (MQ=255)

TTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCC  >  NC_000913/3269145‑3269328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  NC_000913/3269143‑3269329

ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAC                                                                                               >  SRR4302542.1534861/1‑94 (MQ=60)

ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAC                                                                                               >  SRR4302542.1924436/1‑94 (MQ=60)

 TTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACC                                                                                              >  SRR4302542.3850367/1‑94 (MQ=60)

     AATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCC                                                                                          <  SRR4302542.5689508/94‑1 (MQ=60)

     AATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCC                                                                                          >  SRR4302542.2474756/1‑94 (MQ=60)

     AATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCC                                                                                          >  SRR4302542.6209086/1‑94 (MQ=60)

      ATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCT                                                                                         <  SRR4302542.7233805/94‑1 (MQ=60)

      ATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCT                                                                                         <  SRR4302542.5254335/94‑1 (MQ=60)

       TAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTG                                                                                        <  SRR4302542.7584637/94‑1 (MQ=60)

        AAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGG                                                                                       <  SRR4302542.5023182/94‑1 (MQ=60)

        AAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGG                                                                                       >  SRR4302542.1613700/1‑94 (MQ=60)

         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      <  SRR4302542.5706763/94‑1 (MQ=60)

         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      <  SRR4302542.7286257/94‑1 (MQ=60)

         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      >  SRR4302542.1578149/1‑94 (MQ=60)

         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      >  SRR4302542.2512984/1‑94 (MQ=60)

          ACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGT                                                                                     <  SRR4302542.3823450/94‑1 (MQ=60)

           CGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTG                                                                                    >  SRR4302542.1292076/1‑94 (MQ=60)

           CGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTG                                                                                    <  SRR4302542.2605417/94‑1 (MQ=60)

             CGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGC                                                                                  >  SRR4302542.4449107/1‑94 (MQ=60)

                 TTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCA                                                                              >  SRR4302542.7436464/1‑94 (MQ=60)

                   TGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAAT                                                                            <  SRR4302542.3194268/94‑1 (MQ=60)

                    GCGATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATC                                                                           >  SRR4302542.1642739/1‑94 (MQ=60)

                     CCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCA                                                                          >  SRR4302542.7083457/1‑94 (MQ=60)

                     CCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTCCAATCA                                                                          <  SRR4302542.4748637/94‑1 (MQ=60)

                      CATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCAT                                                                         <  SRR4302542.6094392/94‑1 (MQ=60)

                      CATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCAT                                                                         >  SRR4302542.6941877/1‑94 (MQ=60)

                       ATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATC                                                                        <  SRR4302542.4199541/94‑1 (MQ=60)

                       ATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATC                                                                        >  SRR4302542.3977993/1‑94 (MQ=60)

                       ATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATC                                                                        >  SRR4302542.658951/1‑94 (MQ=60)

                         CGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGC                                                                      >  SRR4302542.2118917/1‑94 (MQ=60)

                         CGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGC                                                                      <  SRR4302542.2024761/94‑1 (MQ=60)

                         CGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGC                                                                      >  SRR4302542.4509627/1‑94 (MQ=60)

                            CGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTC                                                                   >  SRR4302542.4789739/1‑94 (MQ=60)

                             GGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCGCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCC                                                                  >  SRR4302542.6282693/1‑94 (MQ=60)

                             GGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCC                                                                  >  SRR4302542.3320348/1‑94 (MQ=60)

                              GAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCA                                                                 <  SRR4302542.2942176/94‑1 (MQ=60)

                               AGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCAC                                                                >  SRR4302542.3598155/1‑94 (MQ=60)

                               AGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAC                                                                >  SRR4302542.7418749/1‑94 (MQ=60)

                                GATCCCCCACCTGGCCTACACTTGCTC‑GCCCAGCGGCCCTGTAACCCAGGCGGGCCGGTCAGCCCCCTGGGTGGCTGCAAGCATCGCTTCCACC                                                               <  SRR4302542.2232219/94‑1 (MQ=60)

                                 ATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCG                                                              >  SRR4302542.4824301/1‑94 (MQ=60)

                                 ATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCG                                                              <  SRR4302542.2809581/94‑1 (MQ=60)

                                 ATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCG                                                              >  SRR4302542.4278541/1‑94 (MQ=60)

                                  TCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGT                                                             <  SRR4302542.5549595/94‑1 (MQ=60)

                                  TCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGT                                                             <  SRR4302542.5251473/94‑1 (MQ=60)

                                    CCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTC                                                           >  SRR4302542.2229985/1‑94 (MQ=60)

                                    CCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTC                                                           >  SRR4302542.4981963/1‑94 (MQ=60)

                                    CCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAACCATCGCTTCCACCGTTC                                                           >  SRR4302542.3617270/1‑94 (MQ=60)

                                     CCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCC                                                          >  SRR4302542.2081493/1‑94 (MQ=60)

                                     CCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCC                                                          >  SRR4302542.1621014/1‑94 (MQ=60)

                                     CCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCC                                                          <  SRR4302542.2764468/94‑1 (MQ=60)

                                       CAACTGGCATTCACTTTCTCGGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCT                                                         >  SRR4302542.6217796/1‑94 (MQ=60)

                                       CAACTGGCATTCACTTTCTC‑GCCCAGCGGCCATGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTT                                                        <  SRR4302542.242383/94‑1 (MQ=60)

                                        AACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTC                                                       >  SRR4302542.5154588/1‑94 (MQ=60)

                                        AACTGGCATTCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTC                                                       <  SRR4302542.3470717/94‑1 (MQ=60)

                                          CTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGC                                                     >  SRR4302542.3324966/1‑94 (MQ=60)

                                           TGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCC                                                    <  SRR4302542.3603648/94‑1 (MQ=60)

                                             GCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAC                                                  >  SRR4302542.5118647/1‑94 (MQ=60)

                                             GCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAC                                                  >  SRR4302542.888512/1‑94 (MQ=60)

                                             GCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCCCCAC                                                  <  SRR4302542.7043808/94‑1 (MQ=60)

                                              CATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACC                                                 <  SRR4302542.4216328/94‑1 (MQ=60)

                                              CATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACC                                                 >  SRR4302542.4801503/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.4580085/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.5324615/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.4843949/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.3344622/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.4351080/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.6259907/1‑94 (MQ=60)

                                               ATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCG                                                >  SRR4302542.4566308/1‑94 (MQ=60)

                                                TTCAATTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGT                                               <  SRR4302542.5783756/94‑1 (MQ=60)

                                                 TCACTTTCTC‑GCCCAGCAGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTC                                              >  SRR4302542.2084736/1‑94 (MQ=60)

                                                  CACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             >  SRR4302542.3906776/1‑94 (MQ=60)

                                                  CACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             <  SRR4302542.3434557/94‑1 (MQ=60)

                                                   ACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGG                                            <  SRR4302542.7363925/94‑1 (MQ=60)

                                                       TCTC‑GCCCAGCGGCCCTGTAGCCCAGGCGTGACGTTCCCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAC                                        <  SRR4302542.354127/94‑1 (MQ=60)

                                                        CTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCCCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACC                                       <  SRR4302542.6929746/94‑1 (MQ=60)

                                                        CTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACC                                       >  SRR4302542.1685795/1‑94 (MQ=60)

                                                         TC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCG                                      >  SRR4302542.970714/1‑94 (MQ=60)

                                                         TC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCG                                      >  SRR4302542.3373532/1‑94 (MQ=60)

                                                          C‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGG                                     <  SRR4302542.2688628/94‑1 (MQ=60)

                                                          C‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGG                                     <  SRR4302542.5580730/94‑1 (MQ=60)

                                                          C‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGG                                     >  SRR4302542.3765662/1‑94 (MQ=60)

                                                            GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGA                                    <  SRR4302542.4554578/94‑1 (MQ=60)

                                                             CCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAA                                   >  SRR4302542.2307150/1‑94 (MQ=60)

                                                             CCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAA                                   >  SRR4302542.6044732/1‑94 (MQ=60)

                                                              CCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAC                                  <  SRR4302542.3531242/94‑1 (MQ=60)

                                                               CAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACA                                 <  SRR4302542.4324965/94‑1 (MQ=60)

                                                               CAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACA                                 <  SRR4302542.1594205/94‑1 (MQ=60)

                                                                AGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAG                                <  SRR4302542.2686705/94‑1 (MQ=60)

                                                                AGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAG                                >  SRR4302542.6936624/1‑94 (MQ=60)

                                                                   GGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAA                             >  SRR4302542.6567252/1‑94 (MQ=60)

                                                                   AGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAA                             >  SRR4302542.5706935/1‑94 (MQ=60)

                                                                    GCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAC                            <  SRR4302542.1155166/94‑1 (MQ=60)

                                                                    GCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAC                            <  SRR4302542.7199739/94‑1 (MQ=60)

                                                                    GCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAC                            <  SRR4302542.2667505/94‑1 (MQ=60)

                                                                    GCCCTGAAACCCAGGCGTGACGTTCTCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAC                            <  SRR4302542.6894229/94‑1 (MQ=60)

                                                                     CCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACG                           <  SRR4302542.6262521/94‑1 (MQ=60)

                                                                      CCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGT                          <  SRR4302542.5828349/94‑1 (MQ=60)

                                                                      CCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGT                          >  SRR4302542.4260409/1‑94 (MQ=60)

                                                                      CCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGT                          >  SRR4302542.2036837/1‑94 (MQ=60)

                                                                       CTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTA                         <  SRR4302542.211349/94‑1 (MQ=60)

                                                                       CTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTA                         >  SRR4302542.2859019/1‑94 (MQ=60)

                                                                       CTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTA                         >  SRR4302542.6836310/1‑94 (MQ=60)

                                                                        TGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAC                        <  SRR4302542.4139275/94‑1 (MQ=60)

                                                                        TGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAC                        >  SRR4302542.5390167/1‑94 (MQ=60)

                                                                         GTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACT                       <  SRR4302542.4511519/94‑1 (MQ=60)

                                                                          TAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTG                      <  SRR4302542.953512/94‑1 (MQ=60)

                                                                            ACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTG                    <  SRR4302542.3196241/94‑1 (MQ=60)

                                                                            ACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTG                    <  SRR4302542.3952885/94‑1 (MQ=60)

                                                                             CCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGC                   >  SRR4302542.400811/1‑94 (MQ=60)

                                                                              CCAGGCGTGACGTTCACCCCCCCGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGCCGGCAACCGGAACAGAAACGTACTGTGCA                  >  SRR4302542.1776424/1‑94 (MQ=60)

                                                                               CAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAT                 <  SRR4302542.6951718/94‑1 (MQ=60)

                                                                                AGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATC                >  SRR4302542.5508515/1‑94 (MQ=60)

                                                                                 GGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCA               <  SRR4302542.6909220/94‑1 (MQ=60)

                                                                                  GCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAG              <  SRR4302542.813427/94‑1 (MQ=60)

                                                                                   CGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGG             <  SRR4302542.3008325/94‑1 (MQ=60)

                                                                                   CGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGG             <  SRR4302542.3781644/94‑1 (MQ=60)

                                                                                       ACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAA         <  SRR4302542.344980/94‑1 (MQ=60)

                                                                                       ACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAA         <  SRR4302542.633331/94‑1 (MQ=60)

                                                                                        CGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAA        >  SRR4302542.1133340/1‑94 (MQ=60)

                                                                                         GTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAT       <  SRR4302542.961298/94‑1 (MQ=60)

                                                                                         GTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAT       <  SRR4302542.1524095/94‑1 (MQ=60)

                                                                                           TCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTT     <  SRR4302542.5499644/94‑1 (MQ=60)

                                                                                            CACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTC    <  SRR4302542.1848661/94‑1 (MQ=60)

                                                                                             AGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCC   <  SRR4302542.3815304/94‑1 (MQ=60)

                                                                                             ACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCC   >  SRR4302542.5889974/1‑94 (MQ=60)

                                                                                              CCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  <  SRR4302542.1022506/94‑1 (MQ=60)

ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTC‑GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  NC_000913/3269143‑3269329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`