Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,282,191 C→T D127D (GAC→GAT)  agaB → PTS system galactosamine‑specific IIB component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,282,1910CT88.0% 889.3 / 72.4 299D127D (GAC→GAT) agaBPTS system galactosamine‑specific IIB component 1
Reads supporting (aligned to +/- strand):  ref base C (14/22);  new base T (126/137);  total (140/159)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 300 total aligned reads displayed.

AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCC  >  NC_000913/3282101‑3282280
                                                                                          |                                                                                          
aGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCa                                                                                          <  1:4133148/93‑1 (MQ=255)
aGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCa                                                                                          >  2:2126971/1‑93 (MQ=255)
  gtggtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAgg                                                                                        <  2:4924080/93‑1 (MQ=255)
  gtggtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAgg                                                                                        <  2:5101323/93‑1 (MQ=255)
   tggtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGa                                                                                       <  1:5269377/93‑1 (MQ=255)
   tggtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGa                                                                                       <  2:1078306/93‑1 (MQ=255)
   tggtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGa                                                                                       <  1:1990521/93‑1 (MQ=255)
     gtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAtc                                                                                     >  1:1664644/1‑93 (MQ=255)
     gtATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAtc                                                                                     >  1:3545736/1‑93 (MQ=255)
      tATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGAtct                                                                                    <  2:4539354/93‑1 (MQ=255)
       aTTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAtctc                                                                                   >  1:5320579/1‑93 (MQ=255)
       aTTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAtctc                                                                                   <  2:4191305/93‑1 (MQ=255)
        ttGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCa                                                                                  <  1:1265201/93‑1 (MQ=255)
        ttGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCa                                                                                  <  2:7296453/93‑1 (MQ=255)
          gACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACg                                                                                >  1:7098750/1‑93 (MQ=255)
             cTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAc                                                                             >  2:3970565/1‑93 (MQ=255)
             cTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAc                                                                             >  2:2501279/1‑93 (MQ=255)
             cTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAc                                                                             >  1:204462/1‑93 (MQ=255)
              tGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACt                                                                            >  1:3400680/1‑93 (MQ=255)
              tGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACt                                                                            >  2:1803549/1‑93 (MQ=255)
                aaaGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTa                                                                          <  2:6919764/93‑1 (MQ=255)
                aaaGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTa                                                                          <  2:4743759/93‑1 (MQ=255)
                  aGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACg                                                                        <  1:3295546/93‑1 (MQ=255)
                  aGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACg                                                                        >  1:6229257/1‑93 (MQ=255)
                  aGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACg                                                                        >  1:4563454/1‑93 (MQ=255)
                   gATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGt                                                                       >  2:581674/1‑93 (MQ=255)
                    aTGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGtt                                                                      <  2:5575892/93‑1 (MQ=255)
                    aTGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGtt                                                                      <  2:3218366/93‑1 (MQ=255)
                     tGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGttt                                                                     <  1:1763782/93‑1 (MQ=255)
                      gTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGtttt                                                                    >  1:7498611/1‑93 (MQ=255)
                         aaCGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTttatt                                                                 <  1:836675/93‑1 (MQ=255)
                          aCGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTttatta                                                                >  1:7443478/1‑93 (MQ=255)
                          aCGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTttatta                                                                >  1:1160791/1‑93 (MQ=255)
                           cGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTaa                                                               >  2:157186/1‑93 (MQ=255)
                            gTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTaaa                                                              <  2:3856098/93‑1 (MQ=255)
                             tCGGCAATATGCATTTCTCGGAAGGGAAATAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaac                                                             <  2:2255627/93‑1 (MQ=255)
                             tCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaac                                                             >  2:343781/1‑93 (MQ=255)
                               ggCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaacaa                                                           >  1:1086689/1‑93 (MQ=255)
                               ggCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaacaa                                                           <  1:7240973/93‑1 (MQ=255)
                               ggCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaacaa                                                           >  1:3727629/1‑93 (MQ=255)
                               ggCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAaacaa                                                           <  2:573199/93‑1 (MQ=255)
                                  aaTATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGt                                                        >  1:5034769/1‑93 (MQ=255)
                                  aaTATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGt                                                        >  2:6777049/1‑93 (MQ=255)
                                  aaTATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGt                                                        >  2:648988/1‑93 (MQ=255)
                                   atatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTg                                                       <  1:2354081/93‑1 (MQ=255)
                                    tatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTgg                                                      >  2:72325/1‑93 (MQ=255)
                                    tatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTgg                                                      >  2:7039903/1‑93 (MQ=255)
                                    tatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTgg                                                      <  2:5314589/93‑1 (MQ=255)
                                    tatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTgg                                                      >  2:5969280/1‑93 (MQ=255)
                                    tatGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTgg                                                      >  2:331510/1‑93 (MQ=255)
                                     atGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGc                                                     >  2:167782/1‑93 (MQ=255)
                                         aTTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑Ga                                                >  2:3797225/1‑93 (MQ=255)
                                           ttCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAAt                                              >  1:3141948/1‑93 (MQ=255)
                                            tctcGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATg                                             >  2:5852137/1‑93 (MQ=255)
                                            tctcGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATg                                             >  1:6016518/1‑93 (MQ=255)
                                              tcGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGtt                                           >  2:2320839/1‑93 (MQ=255)
                                               cGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGttt                                          <  2:6607207/93‑1 (MQ=255)
                                               cGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGttt                                          >  1:1865282/1‑93 (MQ=255)
                                               cGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGttt                                          <  1:4953963/93‑1 (MQ=255)
                                                ggAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGtttt                                         >  1:2587371/1‑93 (MQ=255)
                                                 gAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttc                                        <  1:4613002/93‑1 (MQ=255)
                                                 gAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttc                                        >  1:6119156/1‑93 (MQ=255)
                                                 gAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttc                                        <  2:4348044/93‑1 (MQ=255)
                                                 gAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttc                                        <  1:717185/93‑1 (MQ=255)
                                                  aaGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttca                                       >  2:789068/1‑93 (MQ=255)
                                                      gAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttcattca                                   >  2:3578081/1‑93 (MQ=255)
                                                      gAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTttcattca                                   <  1:1525590/93‑1 (MQ=255)
                                                       aaaaaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAg                                  <  2:522692/93‑1 (MQ=255)
                                                         aaaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGa                                <  1:1827536/93‑1 (MQ=255)
                                                         aaaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGa                                <  2:3087460/93‑1 (MQ=255)
                                                         aaaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGa                                >  1:5548673/1‑93 (MQ=255)
                                                         aaaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGa                                <  1:6680991/93‑1 (MQ=255)
                                                          aaGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGAc                               >  1:1471611/1‑93 (MQ=255)
                                                              aaaTCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTcc                           <  2:2181322/93‑1 (MQ=255)
                                                               aaTCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTccc                          >  2:4102999/1‑93 (MQ=255)
                                                               aaTCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTccc                          <  2:4427793/93‑1 (MQ=255)
                                                               aaTCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTccc                          >  1:2951542/1‑93 (MQ=255)
                                                                aTCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCt                         <  1:4377138/93‑1 (MQ=255)
                                                                aTCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCt                         >  1:5917587/1‑93 (MQ=255)
                                                                 tCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTg                        <  1:23982/93‑1 (MQ=255)
                                                                     cagTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGa                    <  2:2023093/93‑1 (MQ=255)
                                                                      agTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGTCGAATGTTTTCATTCAGGACGTCCCTGGCGa                    >  1:2368840/1‑93 (MQ=255)
                                                                      agTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGAt                   <  2:2440083/93‑1 (MQ=255)
                                                                       gTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATc                  >  2:2793137/1‑93 (MQ=255)
                                                                        tAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCa                 <  2:1258215/93‑1 (MQ=255)
                                                                        tAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCa                 <  1:5771832/93‑1 (MQ=255)
                                                                         aaaGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCaa                >  2:1366252/1‑93 (MQ=255)
                                                                          aaGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCaaa               >  1:6454239/1‑93 (MQ=255)
                                                                             tttATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAg            >  1:1426661/1‑93 (MQ=255)
                                                                             tttATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAg            <  2:2978820/93‑1 (MQ=255)
                                                                                 tGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACa        <  2:5001172/93‑1 (MQ=255)
                                                                                 tGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACa        <  2:6336379/93‑1 (MQ=255)
                                                                                  gTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACaa       >  1:404160/1‑93 (MQ=255)
                                                                                  gTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACaa       >  1:2689817/1‑93 (MQ=255)
                                                                                   tCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACaaa      <  1:4768968/93‑1 (MQ=255)
                                                                                    cGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAAt     <  1:6952105/93‑1 (MQ=255)
                                                                                    cGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAAt     <  1:6342639/93‑1 (MQ=255)
                                                                                       tGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATccc  >  2:6630962/1‑93 (MQ=255)
                                                                                       tGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATccc  <  2:2694495/93‑1 (MQ=255)
                                                                                       tGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATccc  <  1:5579175/93‑1 (MQ=255)
                                                                                          |                                                                                          
AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCC  >  NC_000913/3282101‑3282280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCTGA  >  NC_000913/3282098‑3282283
                                                                                             |                                                                                             
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGAT                                                                                               <  SRR4302542.5377090/94‑1 (MQ=60)
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGAT                                                                                               <  SRR4302542.7200392/94‑1 (MQ=60)
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGAT                                                                                               <  SRR4302542.7250487/94‑1 (MQ=60)
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGAT                                                                                               <  SRR4302542.1469179/94‑1 (MQ=60)
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGAT                                                                                               <  SRR4302542.2134515/94‑1 (MQ=60)
  AAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCA                                                                                             <  SRR4302542.906405/94‑1 (MQ=60)
   AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAG                                                                                            <  SRR4302542.6200344/94‑1 (MQ=60)
   AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAG                                                                                            >  SRR4302542.5051007/1‑94 (MQ=60)
   AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAG                                                                                            <  SRR4302542.4173874/94‑1 (MQ=60)
   AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAG                                                                                            <  SRR4302542.6934408/94‑1 (MQ=60)
   AGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCAGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAG                                                                                            >  SRR4302542.3727417/1‑94 (MQ=60)
      TGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAT                                                                                         <  SRR4302542.4608109/94‑1 (MQ=60)
      TGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAT                                                                                         <  SRR4302542.70417/94‑1 (MQ=60)
      TGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAT                                                                                         <  SRR4302542.2007695/94‑1 (MQ=60)
      TGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGAT                                                                                         <  SRR4302542.5320579/94‑1 (MQ=60)
       GGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATC                                                                                        >  SRR4302542.3578888/1‑94 (MQ=60)
       GGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATC                                                                                        >  SRR4302542.1679023/1‑94 (MQ=60)
       GGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATC                                                                                        <  SRR4302542.2632893/94‑1 (MQ=60)
        GTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCT                                                                                       <  SRR4302542.195217/94‑1 (MQ=60)
         TATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTC                                                                                      >  SRR4302542.5372265/1‑94 (MQ=60)
           TTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCAC                                                                                    <  SRR4302542.1276074/94‑1 (MQ=60)
           TTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCAC                                                                                    <  SRR4302542.4098904/94‑1 (MQ=60)
            TGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACG                                                                                   >  SRR4302542.7168391/1‑94 (MQ=60)
            TGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACG                                                                                   >  SRR4302542.1252751/1‑94 (MQ=60)
            TGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACG                                                                                   >  SRR4302542.4650115/1‑94 (MQ=60)
             GACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGG                                                                                  <  SRR4302542.5646171/94‑1 (MQ=60)
               CCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAC                                                                                <  SRR4302542.2712185/94‑1 (MQ=60)
               CCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAC                                                                                >  SRR4302542.206192/1‑94 (MQ=60)
               CCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAC                                                                                >  SRR4302542.6477030/1‑94 (MQ=60)
               CCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGAC                                                                                >  SRR4302542.1644754/1‑94 (MQ=60)
                CTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACT                                                                               >  SRR4302542.3432571/1‑94 (MQ=60)
                CTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACT                                                                               <  SRR4302542.3352138/94‑1 (MQ=60)
                CTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACT                                                                               >  SRR4302542.4261092/1‑94 (MQ=60)
                CTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACT                                                                               <  SRR4302542.7547884/94‑1 (MQ=60)
                 TGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTT                                                                              <  SRR4302542.6372757/94‑1 (MQ=60)
                   AAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTAC                                                                            >  SRR4302542.3369986/1‑94 (MQ=60)
                   AAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTAC                                                                            >  SRR4302542.6092724/1‑94 (MQ=60)
                    AAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACG                                                                           >  SRR4302542.6290595/1‑94 (MQ=60)
                    AAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACG                                                                           <  SRR4302542.771590/94‑1 (MQ=60)
                    AAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACG                                                                           >  SRR4302542.7218638/1‑94 (MQ=60)
                    AAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACG                                                                           >  SRR4302542.4608129/1‑94 (MQ=60)
                     AGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGT                                                                          <  SRR4302542.3326640/94‑1 (MQ=60)
                      GATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTT                                                                         <  SRR4302542.601768/94‑1 (MQ=60)
                       ATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTT                                                                        <  SRR4302542.6707293/94‑1 (MQ=60)
                        TGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTT                                                                       >  SRR4302542.2475552/1‑94 (MQ=60)
                        TGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTT                                                                       <  SRR4302542.1779082/94‑1 (MQ=60)
                        TGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTT                                                                       >  SRR4302542.7572331/1‑94 (MQ=60)
                           CAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATT                                                                    <  SRR4302542.4767144/94‑1 (MQ=60)
                            AACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTA                                                                   >  SRR4302542.7516359/1‑94 (MQ=60)
                            AACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTA                                                                   >  SRR4302542.1795137/1‑94 (MQ=60)
                            AACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTA                                                                   >  SRR4302542.4802626/1‑94 (MQ=60)
                            AACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTA                                                                   >  SRR4302542.1170748/1‑94 (MQ=60)
                            AACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTA                                                                   <  SRR4302542.843903/94‑1 (MQ=60)
                             ACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAA                                                                  <  SRR4302542.1892837/94‑1 (MQ=60)
                              CGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAA                                                                 >  SRR4302542.6208655/1‑94 (MQ=60)
                              CGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAA                                                                 >  SRR4302542.535771/1‑94 (MQ=60)
                               GTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAAC                                                                <  SRR4302542.6702921/94‑1 (MQ=60)
                               GTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAAC                                                                >  SRR4302542.1385845/1‑94 (MQ=60)
                               GTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAAC                                                                <  SRR4302542.2033391/94‑1 (MQ=60)
                                 CGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAA                                                              >  SRR4302542.1096009/1‑94 (MQ=60)
                                 CGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAA                                                              >  SRR4302542.3764544/1‑94 (MQ=60)
                                  GGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAAC                                                             <  SRR4302542.7311987/94‑1 (MQ=60)
                                  GGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAAC                                                             <  SRR4302542.1396324/94‑1 (MQ=60)
                                   GCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACG                                                            <  SRR4302542.3311892/94‑1 (MQ=60)
                                    CAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGT                                                           <  SRR4302542.1633747/94‑1 (MQ=60)
                                    CAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGT                                                           >  SRR4302542.5083651/1‑94 (MQ=60)
                                    CAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGT                                                           <  SRR4302542.153219/94‑1 (MQ=60)
                                     AATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTG                                                          >  SRR4302542.503512/1‑94 (MQ=60)
                                     AATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTG                                                          <  SRR4302542.7328365/94‑1 (MQ=60)
                                      ATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGG                                                         <  SRR4302542.5569942/94‑1 (MQ=60)
                                      ATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGG                                                         >  SRR4302542.204172/1‑94 (MQ=60)
                                      ATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGG                                                         <  SRR4302542.2374299/94‑1 (MQ=60)
                                        ATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCG                                                       <  SRR4302542.4408100/94‑1 (MQ=60)
                                        ATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCG                                                       >  SRR4302542.7245497/1‑94 (MQ=60)
                                        ATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCG                                                       <  SRR4302542.2406605/94‑1 (MQ=60)
                                        ATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCG                                                       <  SRR4302542.241028/94‑1 (MQ=60)
                                         TGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT                                                      >  SRR4302542.3801178/1‑94 (MQ=60)
                                         TGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT                                                      >  SRR4302542.2791571/1‑94 (MQ=60)
                                          GCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑G                                                    <  SRR4302542.1442296/94‑1 (MQ=60)
                                           CATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GA                                                   >  SRR4302542.1169964/1‑94 (MQ=60)
                                           CATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GA                                                   >  SRR4302542.349105/1‑94 (MQ=60)
                                             TTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAAT                                                 >  SRR4302542.3171804/1‑94 (MQ=60)
                                             TTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAAT                                                 <  SRR4302542.5621749/94‑1 (MQ=60)
                                              TTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATG                                                <  SRR4302542.2384517/94‑1 (MQ=60)
                                              TTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATG                                                >  SRR4302542.6074783/1‑94 (MQ=60)
                                              TTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATG                                                <  SRR4302542.3045005/94‑1 (MQ=60)
                                               TCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGT                                               <  SRR4302542.235453/94‑1 (MQ=60)
                                                 TCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTT                                             >  SRR4302542.1881417/1‑94 (MQ=60)
                                                  CGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTT                                            >  SRR4302542.2612442/1‑94 (MQ=60)
                                                  CGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTT                                            >  SRR4302542.5276583/1‑94 (MQ=60)
                                                  CGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTT                                            <  SRR4302542.5002098/94‑1 (MQ=60)
                                                   GGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTC                                           >  SRR4302542.6178363/1‑94 (MQ=60)
                                                   GGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTC                                           >  SRR4302542.3514553/1‑94 (MQ=60)
                                                   GGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTC                                           >  SRR4302542.180226/1‑94 (MQ=60)
                                                    GAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCA                                          <  SRR4302542.7192975/94‑1 (MQ=60)
                                                    GAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCA                                          <  SRR4302542.4658070/94‑1 (MQ=60)
                                                    GAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCA                                          <  SRR4302542.723370/94‑1 (MQ=60)
                                                     AAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCAT                                         >  SRR4302542.2797025/1‑94 (MQ=60)
                                                      AGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATT                                        >  SRR4302542.7293619/1‑94 (MQ=60)
                                                      AGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATT                                        >  SRR4302542.359751/1‑94 (MQ=60)
                                                       GGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTC                                       <  SRR4302542.3786091/94‑1 (MQ=60)
                                                        GGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCA                                      >  SRR4302542.978464/1‑94 (MQ=60)
                                                        GGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCA                                      <  SRR4302542.1472136/94‑1 (MQ=60)
                                                         GAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAG                                     <  SRR4302542.1538760/94‑1 (MQ=60)
                                                         GAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAG                                     <  SRR4302542.2574663/94‑1 (MQ=60)
                                                         GAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAG                                     <  SRR4302542.4475493/94‑1 (MQ=60)
                                                          AAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGG                                    <  SRR4302542.6659528/94‑1 (MQ=60)
                                                           AAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGA                                   >  SRR4302542.5602590/1‑94 (MQ=60)
                                                           AAAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGA                                   >  SRR4302542.7548964/1‑94 (MQ=60)
                                                            AAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGAC                                  >  SRR4302542.1484317/1‑94 (MQ=60)
                                                            AAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGAC                                  <  SRR4302542.1843363/94‑1 (MQ=60)
                                                            AAAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGAC                                  <  SRR4302542.6746808/94‑1 (MQ=60)
                                                             AAGCAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACG                                 >  SRR4302542.829648/1‑94 (MQ=60)
                                                                CAAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCC                              >  SRR4302542.5342010/1‑94 (MQ=60)
                                                                 AAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCC                             >  SRR4302542.1222047/1‑94 (MQ=60)
                                                                 AAATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCC                             >  SRR4302542.2979796/1‑94 (MQ=60)
                                                                  AATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCT                            <  SRR4302542.3155074/94‑1 (MQ=60)
                                                                  AATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCT                            <  SRR4302542.3196212/94‑1 (MQ=60)
                                                                  AATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCT                            >  SRR4302542.5974937/1‑94 (MQ=60)
                                                                   ATCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTG                           <  SRR4302542.4420097/94‑1 (MQ=60)
                                                                    TCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGG                          <  SRR4302542.24187/94‑1 (MQ=60)
                                                                    TCAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGG                          <  SRR4302542.5934096/94‑1 (MQ=60)
                                                                     CAGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGC                         <  SRR4302542.6307309/94‑1 (MQ=60)
                                                                      AGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCG                        <  SRR4302542.6124316/94‑1 (MQ=60)
                                                                      AGCAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCG                        >  SRR4302542.5429230/1‑94 (MQ=60)
                                                                        CAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGTCGAATGTTTTCATTCAGGACGTCCCTGGCGA                       >  SRR4302542.2389502/1‑94 (MQ=60)
                                                                        CAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGAT                      <  SRR4302542.2385240/94‑1 (MQ=60)
                                                                        CAGTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGAT                      <  SRR4302542.1735250/94‑1 (MQ=60)
                                                                          GTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCA                    >  SRR4302542.5602648/1‑94 (MQ=60)
                                                                          GTAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCA                    <  SRR4302542.5266589/94‑1 (MQ=60)
                                                                           TAAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAA                   <  SRR4302542.5827855/94‑1 (MQ=60)
                                                                            AAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAA                  >  SRR4302542.6517788/1‑94 (MQ=60)
                                                                            AAAGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAA                  >  SRR4302542.5843834/1‑94 (MQ=60)
                                                                              AGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAA                >  SRR4302542.1874087/1‑94 (MQ=60)
                                                                              AGTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAA                >  SRR4302542.7356834/1‑94 (MQ=60)
                                                                               GTTTATGTCGATGATCAGGATCTCACGTACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAG               <  SRR4302542.1120140/94‑1 (MQ=60)
                                                                               GTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAG               <  SRR4302542.707400/94‑1 (MQ=60)
                                                                               GTTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAG               >  SRR4302542.1439008/1‑94 (MQ=60)
                                                                               GTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAG               <  SRR4302542.6844182/94‑1 (MQ=60)
                                                                               GTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAG               >  SRR4302542.970655/1‑94 (MQ=60)
                                                                                TTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGA              <  SRR4302542.18631/94‑1 (MQ=60)
                                                                                TTTATGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGA              >  SRR4302542.4102058/1‑94 (MQ=60)
                                                                                  TATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAAC            <  SRR4302542.1736619/94‑1 (MQ=60)
                                                                                    TGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAA          >  SRR4302542.2715754/1‑94 (MQ=60)
                                                                                    TGTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAA          >  SRR4302542.407649/1‑94 (MQ=60)
                                                                                     GTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAA         <  SRR4302542.6006360/94‑1 (MQ=60)
                                                                                     GTCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAA         >  SRR4302542.2240309/1‑94 (MQ=60)
                                                                                     GTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAA         <  SRR4302542.4982359/94‑1 (MQ=60)
                                                                                      TCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAAT        <  SRR4302542.4815450/94‑1 (MQ=60)
                                                                                      TCGATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAAT        <  SRR4302542.4630569/94‑1 (MQ=60)
                                                                                       CGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATC       <  SRR4302542.6405126/94‑1 (MQ=60)
                                                                                       CGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATC       <  SRR4302542.7020415/94‑1 (MQ=60)
                                                                                         ATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCC     <  SRR4302542.5799183/94‑1 (MQ=60)
                                                                                         ATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCC     <  SRR4302542.1510786/94‑1 (MQ=60)
                                                                                         ATGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCC     <  SRR4302542.4830451/94‑1 (MQ=60)
                                                                                          TGATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCT    <  SRR4302542.5633393/94‑1 (MQ=60)
                                                                                           GATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCTG   <  SRR4302542.558131/94‑1 (MQ=60)
                                                                                            ATCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCTGA  <  SRR4302542.669546/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGAAGGTGGTATTGACCTGAAAGATGTCAACGTCGGCAATATGCATTTCTCGGAAGGGAAAAAGCAAATCAGCAGTAAAGTTTATGTCGATGACCAGGATCTCACGGACTTACGTTTTATTAAACAACGTGGCGT‑GAATGTTTTCATTCAGGACGTCCCTGGCGATCAAAAAGAACAAATCCCTGA  >  NC_000913/3282098‑3282283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑