Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,291,253 T→C R71R (CGT→CGC)  yraM → putative glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,2530TC81.8% 603.4 / 137.0 286R71R (CGT→CGC) yraMputative glycosylase
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base C (113/121);  total (139/147)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 287 total aligned reads displayed.

CACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA  >  NC_000913/3291163‑3291344
                                                                                          |                                                                                           
 aCGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCa                                                                                          <  1:5981021/93‑1 (MQ=255)
 aCGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCa                                                                                          <  1:4521269/93‑1 (MQ=255)
  cGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCAc                                                                                         >  1:285858/1‑93 (MQ=255)
    gcgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTg                                                                                       >  2:2487578/1‑93 (MQ=255)
     cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTgg                                                                                      <  2:5040208/93‑1 (MQ=255)
      gcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGt                                                                                     <  2:3097658/93‑1 (MQ=255)
       cAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTg                                                                                    <  1:464602/93‑1 (MQ=255)
          gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGaaa                                                                                 <  1:5065524/93‑1 (MQ=255)
          gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGaaa                                                                                 >  1:3591641/1‑93 (MQ=255)
          gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGaaa                                                                                 <  2:740003/93‑1 (MQ=255)
          gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGaaa                                                                                 <  1:2974122/93‑1 (MQ=255)
          gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGaaa                                                                                 <  2:6968624/93‑1 (MQ=255)
           cTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAaag                                                                                >  2:5858545/1‑93 (MQ=255)
            tGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAaaga                                                                               >  1:2206079/1‑93 (MQ=255)
             gATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAaagaa                                                                              >  2:5811907/1‑93 (MQ=255)
             gATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAaagaa                                                                              <  1:2010510/93‑1 (MQ=255)
               ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAgg                                                                            <  1:1549546/93‑1 (MQ=255)
               ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAgg                                                                            >  2:1459839/1‑93 (MQ=255)
               ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAgg                                                                            >  2:1851023/1‑93 (MQ=255)
                 cTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTa                                                                          <  2:5264240/93‑1 (MQ=255)
                  tGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTaa                                                                         <  2:210582/93‑1 (MQ=255)
                  tGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTaa                                                                         >  2:5381725/1‑93 (MQ=255)
                  tGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTaa                                                                         >  2:127826/1‑93 (MQ=255)
                    ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTaaaa                                                                       <  1:6776004/93‑1 (MQ=255)
                     cTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAAc                                                                      <  1:5133633/93‑1 (MQ=255)
                     cTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAAc                                                                      <  2:705946/93‑1 (MQ=255)
                       tttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTGCTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCg                                                                    <  2:425068/93‑1 (MQ=255)
                       tttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCg                                                                    >  2:500438/1‑93 (MQ=255)
                       tttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCg                                                                    <  2:5398295/93‑1 (MQ=255)
                          aTCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGc                                                                 <  1:673324/93‑1 (MQ=255)
                           tCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCa                                                                >  2:164527/1‑93 (MQ=255)
                           tCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCa                                                                >  2:5724248/1‑93 (MQ=255)
                           tCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCa                                                                <  2:6480325/93‑1 (MQ=255)
                           tCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCa                                                                <  1:1819789/93‑1 (MQ=255)
                             ttCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAgg                                                              >  1:3462153/1‑93 (MQ=255)
                              tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGc                                                             <  1:6720971/93‑1 (MQ=255)
                               cagcagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCg                                                            >  2:5503141/1‑93 (MQ=255)
                                agcagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCgg                                                           >  1:7190975/1‑93 (MQ=255)
                                 gcagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGt                                                          >  1:5425995/1‑93 (MQ=255)
                                   agATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTg                                                        <  2:3587378/93‑1 (MQ=255)
                                    gATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGa                                                       >  2:2848683/1‑93 (MQ=255)
                                    gATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGa                                                       >  1:477823/1‑93 (MQ=255)
                                     aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAg                                                      <  2:732808/93‑1 (MQ=255)
                                       gcagcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgtt                                                    >  1:568635/1‑93 (MQ=255)
                                        cagcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttg                                                   <  1:4390910/93‑1 (MQ=255)
                                         agcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttgt                                                  >  2:2537635/1‑93 (MQ=255)
                                         agcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttgt                                                  >  2:4437170/1‑93 (MQ=255)
                                          gcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttgtt                                                 >  2:2185784/1‑93 (MQ=255)
                                          gcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttgtt                                                 >  1:2231810/1‑93 (MQ=255)
                                          gcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAgttgtt                                                 <  2:3694403/93‑1 (MQ=255)
                                           caAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGttt                                                >  1:3907444/1‑93 (MQ=255)
                                           caAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGttt                                                <  1:4716491/93‑1 (MQ=255)
                                            aaaGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTa                                               >  1:256478/1‑93 (MQ=255)
                                            aaaGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTa                                               >  2:2625461/1‑93 (MQ=255)
                                             aaGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTaa                                              <  1:2673025/93‑1 (MQ=255)
                                             aaGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTaa                                              >  1:6940380/1‑93 (MQ=255)
                                              aGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAc                                             <  2:340804/93‑1 (MQ=255)
                                              aGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAc                                             <  1:3275864/93‑1 (MQ=255)
                                               gCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAcc                                            >  2:1929560/1‑93 (MQ=255)
                                               gCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAcc                                            >  1:7427870/1‑93 (MQ=255)
                                               gCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAcc                                            >  2:6874960/1‑93 (MQ=255)
                                                ctctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCa                                           >  2:2740750/1‑93 (MQ=255)
                                                    gatgatACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTa                                       >  2:2312491/1‑93 (MQ=255)
                                                    gatgatACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTa                                       >  1:416402/1‑93 (MQ=255)
                                                     atgatACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAc                                      <  2:3342117/93‑1 (MQ=255)
                                                     atgatACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAc                                      <  2:6682593/93‑1 (MQ=255)
                                                     atgatACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAc                                      <  2:1049761/93‑1 (MQ=255)
                                                        atACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGc                                   >  2:6352533/1‑93 (MQ=255)
                                                         tACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCa                                  <  1:5894885/93‑1 (MQ=255)
                                                          aCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCaa                                 <  2:7256449/93‑1 (MQ=255)
                                                             aGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGaa                              >  1:1512611/1‑93 (MQ=255)
                                                              ggATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAAc                             >  1:101475/1‑93 (MQ=255)
                                                               gATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACt                            >  2:4750701/1‑93 (MQ=255)
                                                                aTCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTg                           >  1:7075348/1‑93 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          <  2:1715796/93‑1 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          <  1:994872/93‑1 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          >  1:894062/1‑93 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          <  2:5782729/93‑1 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          >  1:6549740/1‑93 (MQ=255)
                                                                 tCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGa                          <  1:6080942/93‑1 (MQ=255)
                                                                  cAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGaa                         <  1:1673385/93‑1 (MQ=255)
                                                                   aaCTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAAc                        >  1:4294204/1‑93 (MQ=255)
                                                                     cTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGa                      <  2:3952875/93‑1 (MQ=255)
                                                                     cTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCACAAGAACTGAACGa                      <  2:7488312/93‑1 (MQ=255)
                                                                      tGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGAt                     <  2:4508218/93‑1 (MQ=255)
                                                                      tGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGAt                     >  2:3131907/1‑93 (MQ=255)
                                                                      tGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGAt                     >  1:403482/1‑93 (MQ=255)
                                                                          aaTTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTc                 >  2:3490370/1‑93 (MQ=255)
                                                                           aTTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCa                <  1:2371422/93‑1 (MQ=255)
                                                                           aTTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCa                >  2:847801/1‑93 (MQ=255)
                                                                             tACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGc              <  2:3236439/93‑1 (MQ=255)
                                                                               cTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGt            <  1:2354897/93‑1 (MQ=255)
                                                                               cTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGt            <  1:1423483/93‑1 (MQ=255)
                                                                                tCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTc           >  2:6625289/1‑93 (MQ=255)
                                                                                tCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTc           >  1:339028/1‑93 (MQ=255)
                                                                                tCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTc           <  2:1488015/93‑1 (MQ=255)
                                                                                tCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGATGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTc           >  2:6263981/1‑93 (MQ=255)
                                                                                  gCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTcgc         <  1:4876897/93‑1 (MQ=255)
                                                                                         gTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGaaaa  <  2:854614/93‑1 (MQ=255)
                                                                                         gTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGaaaa  >  1:1311588/1‑93 (MQ=255)
                                                                                          |                                                                                           
CACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA  >  NC_000913/3291163‑3291344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACA  >  NC_000913/3291160‑3291346
                                                                                             |                                                                                             
GGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGT                                                                                               <  SRR4302542.3245173/94‑1 (MQ=60)
GGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGC                                                                                               >  SRR4302542.3692767/1‑94 (MQ=60)
 GGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCG                                                                                              <  SRR4302542.6784343/94‑1 (MQ=60)
 GGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCG                                                                                              <  SRR4302542.1581814/94‑1 (MQ=60)
   CACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCA                                                                                            <  SRR4302542.3707490/94‑1 (MQ=60)
    ACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCAC                                                                                           <  SRR4302542.4565565/94‑1 (MQ=60)
    ACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCAC                                                                                           <  SRR4302542.5034253/94‑1 (MQ=60)
    ACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCAC                                                                                           >  SRR4302542.288348/1‑94 (MQ=60)
    ACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCAC                                                                                           <  SRR4302542.6038958/94‑1 (MQ=60)
      GGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTG                                                                                         <  SRR4302542.541618/94‑1 (MQ=60)
       GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGG                                                                                        >  SRR4302542.2696156/1‑94 (MQ=60)
       GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGG                                                                                        <  SRR4302542.6813119/94‑1 (MQ=60)
       GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGG                                                                                        <  SRR4302542.6209468/94‑1 (MQ=60)
       GCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGG                                                                                        <  SRR4302542.4770090/94‑1 (MQ=60)
        CGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGT                                                                                       >  SRR4302542.6653743/1‑94 (MQ=60)
         GCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTG                                                                                      >  SRR4302542.7505043/1‑94 (MQ=60)
          CAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGA                                                                                     >  SRR4302542.255740/1‑94 (MQ=60)
          CAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGA                                                                                     >  SRR4302542.154539/1‑94 (MQ=60)
          CAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGA                                                                                     >  SRR4302542.6752843/1‑94 (MQ=60)
          CAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGA                                                                                     <  SRR4302542.468626/94‑1 (MQ=60)
           AGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAA                                                                                    <  SRR4302542.1988775/94‑1 (MQ=60)
           AGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAA                                                                                    >  SRR4302542.2387407/1‑94 (MQ=60)
            GGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA                                                                                   >  SRR4302542.4340261/1‑94 (MQ=60)
            GGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAA                                                                                   >  SRR4302542.3625139/1‑94 (MQ=60)
             GCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAG                                                                                  <  SRR4302542.5114754/94‑1 (MQ=60)
             GCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAG                                                                                  <  SRR4302542.1395990/94‑1 (MQ=60)
             GCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAG                                                                                  <  SRR4302542.3002604/94‑1 (MQ=60)
              CTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGA                                                                                 <  SRR4302542.1932497/94‑1 (MQ=60)
              CTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGA                                                                                 >  SRR4302542.2225058/1‑94 (MQ=60)
               TGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAA                                                                                >  SRR4302542.6071098/1‑94 (MQ=60)
               TGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAA                                                                                >  SRR4302542.1357201/1‑94 (MQ=60)
                GATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAG                                                                               <  SRR4302542.2027874/94‑1 (MQ=60)
                GATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAG                                                                               >  SRR4302542.575006/1‑94 (MQ=60)
                GATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAG                                                                               <  SRR4302542.249764/94‑1 (MQ=60)
                  TTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGT                                                                             <  SRR4302542.1811931/94‑1 (MQ=60)
                  TTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGT                                                                             <  SRR4302542.1562916/94‑1 (MQ=60)
                  TTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGT                                                                             <  SRR4302542.5389838/94‑1 (MQ=60)
                      GCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAA                                                                         >  SRR4302542.4727683/1‑94 (MQ=60)
                      GCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAA                                                                         <  SRR4302542.4857349/94‑1 (MQ=60)
                       CCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAAC                                                                        >  SRR4302542.7327131/1‑94 (MQ=60)
                       CCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAAC                                                                        <  SRR4302542.6842661/94‑1 (MQ=60)
                       CCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAAC                                                                        >  SRR4302542.1649502/1‑94 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTCCTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.3389410/94‑1 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.2291485/94‑1 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACC                                                                       >  SRR4302542.6800748/1‑94 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.4865974/94‑1 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.2932512/94‑1 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.3439976/94‑1 (MQ=60)
                        CTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACC                                                                       <  SRR4302542.5183537/94‑1 (MQ=60)
                         TTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCG                                                                      <  SRR4302542.7538462/94‑1 (MQ=60)
                           TTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGG                                                                    <  SRR4302542.5589759/94‑1 (MQ=60)
                            TATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGC                                                                   >  SRR4302542.2240393/1‑94 (MQ=60)
                            TATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGC                                                                   <  SRR4302542.1515354/94‑1 (MQ=60)
                             ATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCA                                                                  <  SRR4302542.679134/94‑1 (MQ=60)
                             ATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCA                                                                  >  SRR4302542.941540/1‑94 (MQ=60)
                              TCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAG                                                                 <  SRR4302542.1835545/94‑1 (MQ=60)
                               CTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGG                                                                >  SRR4302542.3494574/1‑94 (MQ=60)
                               CTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGG                                                                <  SRR4302542.4612673/94‑1 (MQ=60)
                                TTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGC                                                               <  SRR4302542.4538435/94‑1 (MQ=60)
                                 TCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCG                                                              <  SRR4302542.6787160/94‑1 (MQ=60)
                                  CAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGG                                                             >  SRR4302542.7261530/1‑94 (MQ=60)
                                   AGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGT                                                            >  SRR4302542.5478722/1‑94 (MQ=60)
                                   AGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGT                                                            <  SRR4302542.7082508/94‑1 (MQ=60)
                                   AGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGT                                                            >  SRR4302542.3608561/1‑94 (MQ=60)
                                    GCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTTCTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTT                                                           <  SRR4302542.1099477/94‑1 (MQ=60)
                                     CAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTG                                                          >  SRR4302542.973601/1‑94 (MQ=60)
                                      AGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGA                                                         >  SRR4302542.481966/1‑94 (MQ=60)
                                         TGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTT                                                      <  SRR4302542.402675/94‑1 (MQ=60)
                                         TGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTT                                                      >  SRR4302542.573526/1‑94 (MQ=60)
                                           CAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGT                                                    <  SRR4302542.4433989/94‑1 (MQ=60)
                                            AGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTT                                                   <  SRR4302542.7441351/94‑1 (MQ=60)
                                            AGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTT                                                   >  SRR4302542.6111041/1‑94 (MQ=60)
                                            AGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTT                                                   >  SRR4302542.2251015/1‑94 (MQ=60)
                                             GCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTT                                                  >  SRR4302542.3946015/1‑94 (MQ=60)
                                             GCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTT                                                  <  SRR4302542.4314793/94‑1 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 >  SRR4302542.2381406/1‑94 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 >  SRR4302542.2824445/1‑94 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 >  SRR4302542.258664/1‑94 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 >  SRR4302542.678834/1‑94 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 <  SRR4302542.4762467/94‑1 (MQ=60)
                                              CAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTA                                                 <  SRR4302542.39705/94‑1 (MQ=60)
                                               AAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA                                                >  SRR4302542.7008568/1‑94 (MQ=60)
                                                AAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAC                                               <  SRR4302542.2698818/94‑1 (MQ=60)
                                                 AGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACC                                              <  SRR4302542.3306775/94‑1 (MQ=60)
                                                 AGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACC                                              >  SRR4302542.7500558/1‑94 (MQ=60)
                                                   CTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAA                                            >  SRR4302542.1004478/1‑94 (MQ=60)
                                                   CTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAA                                            >  SRR4302542.3213800/1‑94 (MQ=60)
                                                   CTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAA                                            >  SRR4302542.144403/1‑94 (MQ=60)
                                                     CTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACT                                          <  SRR4302542.7217607/94‑1 (MQ=60)
                                                     CTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACT                                          <  SRR4302542.690999/94‑1 (MQ=60)
                                                      TGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTA                                         >  SRR4302542.7107396/1‑94 (MQ=60)
                                                      TGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTA                                         >  SRR4302542.419989/1‑94 (MQ=60)
                                                       GATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC                                        >  SRR4302542.4270959/1‑94 (MQ=60)
                                                        ATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACC                                       <  SRR4302542.581406/94‑1 (MQ=60)
                                                        ATGATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACC                                       >  SRR4302542.2171969/1‑94 (MQ=60)
                                                           ATACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCA                                    >  SRR4302542.1554623/1‑94 (MQ=60)
                                                            TACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAA                                   >  SRR4302542.3497816/1‑94 (MQ=60)
                                                            TACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAA                                   <  SRR4302542.5952016/94‑1 (MQ=60)
                                                             ACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAG                                  >  SRR4302542.7226091/1‑94 (MQ=60)
                                                             ACCAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAG                                  <  SRR4302542.4179098/94‑1 (MQ=60)
                                                               CAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAA                                >  SRR4302542.1711966/1‑94 (MQ=60)
                                                               CAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAA                                >  SRR4302542.5544456/1‑94 (MQ=60)
                                                               CAGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAA                                >  SRR4302542.1525675/1‑94 (MQ=60)
                                                                AGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGATGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAAC                               >  SRR4302542.692957/1‑94 (MQ=60)
                                                                AGGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAAC                               >  SRR4302542.102323/1‑94 (MQ=60)
                                                                 GGATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCACCTACCGCAAGAACT                              >  SRR4302542.3483584/1‑94 (MQ=60)
                                                                  GATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTG                             >  SRR4302542.7144789/1‑94 (MQ=60)
                                                                   ATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGA                            >  SRR4302542.6614316/1‑94 (MQ=60)
                                                                   ATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGA                            >  SRR4302542.901801/1‑94 (MQ=60)
                                                                   ATCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGA                            <  SRR4302542.2969067/94‑1 (MQ=60)
                                                                    TCAATTGGCAATTCCTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAA                           <  SRR4302542.3748455/94‑1 (MQ=60)
                                                                    TCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAA                           <  SRR4302542.1003434/94‑1 (MQ=60)
                                                                    TCAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAA                           <  SRR4302542.6139818/94‑1 (MQ=60)
                                                                     CAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAAC                          <  SRR4302542.1687847/94‑1 (MQ=60)
                                                                     CAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAAC                          <  SRR4302542.4113304/94‑1 (MQ=60)
                                                                     CAACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAAC                          >  SRR4302542.4336441/1‑94 (MQ=60)
                                                                      AACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACG                         <  SRR4302542.463294/94‑1 (MQ=60)
                                                                       ACTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGA                        <  SRR4302542.2769755/94‑1 (MQ=60)
                                                                        CTGGCAATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGAT                       >  SRR4302542.406967/1‑94 (MQ=60)
                                                                              ATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAG                 <  SRR4302542.4753230/94‑1 (MQ=60)
                                                                              ATTACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAG                 <  SRR4302542.2392273/94‑1 (MQ=60)
                                                                               TTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGC                >  SRR4302542.4525644/1‑94 (MQ=60)
                                                                                TACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCG               <  SRR4302542.1571721/94‑1 (MQ=60)
                                                                                 ACTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGT              <  SRR4302542.4787742/94‑1 (MQ=60)
                                                                                  CTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTC             <  SRR4302542.2375127/94‑1 (MQ=60)
                                                                                  CTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTC             >  SRR4302542.5084497/1‑94 (MQ=60)
                                                                                  CTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTC             <  SRR4302542.1435808/94‑1 (MQ=60)
                                                                                  CTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTC             >  SRR4302542.341969/1‑94 (MQ=60)
                                                                                  CTCGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTC             <  SRR4302542.2605082/94‑1 (MQ=60)
                                                                                    CGCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGC           >  SRR4302542.6018536/1‑94 (MQ=60)
                                                                                     GCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCG          <  SRR4302542.6441036/94‑1 (MQ=60)
                                                                                     GCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCG          >  SRR4302542.226279/1‑94 (MQ=60)
                                                                                     GCCATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCG          <  SRR4302542.4924361/94‑1 (MQ=60)
                                                                                       CATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAG        <  SRR4302542.5177474/94‑1 (MQ=60)
                                                                                        ATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGA       >  SRR4302542.6007912/1‑94 (MQ=60)
                                                                                        ATTCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGA       >  SRR4302542.2229506/1‑94 (MQ=60)
                                                                                          TCGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAA     <  SRR4302542.3344008/94‑1 (MQ=60)
                                                                                           CGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA    >  SRR4302542.1322890/1‑94 (MQ=60)
                                                                                           CGCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA    >  SRR4302542.612216/1‑94 (MQ=60)
                                                                                            GCGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAAC   <  SRR4302542.5149180/94‑1 (MQ=60)
                                                                                             CGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACA  >  SRR4302542.5824430/1‑94 (MQ=60)
                                                                                             CGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACA  <  SRR4302542.5464912/94‑1 (MQ=60)
                                                                                             CGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACA  <  SRR4302542.6240064/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACA  >  NC_000913/3291160‑3291346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑