Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,312,106 T→C Y517C (TAC→TGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,312,1060TC82.3% 673.1 / 135.9 311Y517C (TAC→TGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (26/29);  new base C (128/128);  total (154/157)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT  >  NC_000913/3312015‑3312197
                                                                                           |                                                                                           
  cGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACt                                                                                          <  2:5975993/93‑1 (MQ=255)
  cGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACt                                                                                          <  1:2712440/93‑1 (MQ=255)
   gTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTg                                                                                         >  2:1983789/1‑93 (MQ=255)
    tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTgg                                                                                        >  2:1217066/1‑93 (MQ=255)
     ccAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTggg                                                                                       >  2:4248078/1‑93 (MQ=255)
      cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                      >  1:6997614/1‑93 (MQ=255)
      cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                      >  2:3265484/1‑93 (MQ=255)
      cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                      <  2:4146907/93‑1 (MQ=255)
       agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGgag                                                                                     >  2:2662066/1‑93 (MQ=255)
       agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgag                                                                                     >  2:2574713/1‑93 (MQ=255)
        gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgaga                                                                                    <  1:1127340/93‑1 (MQ=255)
        gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgaga                                                                                    >  2:1292499/1‑93 (MQ=255)
          agTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgt                                                                                  <  1:1750681/93‑1 (MQ=255)
           gTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgtt                                                                                 >  1:6338267/1‑93 (MQ=255)
           gTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgtt                                                                                 <  2:3455308/93‑1 (MQ=255)
            ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttc                                                                                <  2:2491200/93‑1 (MQ=255)
            ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttc                                                                                >  1:6287223/1‑93 (MQ=255)
             tCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcg                                                                               <  1:1439058/93‑1 (MQ=255)
              cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgt                                                                              >  2:4845731/1‑93 (MQ=255)
                tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                            >  2:5079557/1‑93 (MQ=255)
                tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                            >  1:4431817/1‑93 (MQ=255)
                  gATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCtt                                                                          >  2:5736529/1‑93 (MQ=255)
                  gATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCtt                                                                          <  1:7211469/93‑1 (MQ=255)
                    tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTaa                                                                        >  1:1882674/1‑93 (MQ=255)
                    tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTaa                                                                        <  1:4326022/93‑1 (MQ=255)
                    tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTaa                                                                        <  2:6314922/93‑1 (MQ=255)
                    tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTaa                                                                        <  2:7073692/93‑1 (MQ=255)
                     aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAc                                                                       <  1:3668863/93‑1 (MQ=255)
                     aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAc                                                                       <  2:3986098/93‑1 (MQ=255)
                        ggTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgcg                                                                    >  2:1276039/1‑93 (MQ=255)
                        ggTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgcg                                                                    >  1:3508928/1‑93 (MQ=255)
                          tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                  >  2:4587990/1‑93 (MQ=255)
                          tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                  >  1:2916813/1‑93 (MQ=255)
                          tACCCGCTTTCGCAGATACGTGTACGAACTGGCCTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                  <  2:910072/93‑1 (MQ=255)
                            cccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGTGAGAGTTCGCTCTTAACGCGAAcc                                                                >  1:5450491/1‑93 (MQ=255)
                            cccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATcc                                                                <  1:5567242/93‑1 (MQ=255)
                              cGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCgg                                                              <  2:6457972/93‑1 (MQ=255)
                               gCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGa                                                             <  1:6756225/93‑1 (MQ=255)
                                 tttCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATc                                                           <  1:2034618/93‑1 (MQ=255)
                                 tttCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATc                                                           >  2:1885573/1‑93 (MQ=255)
                                  ttCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCa                                                          <  2:1162601/93‑1 (MQ=255)
                                  ttCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCa                                                          <  2:5337779/93‑1 (MQ=255)
                                   tCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg                                                         <  2:2407619/93‑1 (MQ=255)
                                    cGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGc                                                        <  1:226958/93‑1 (MQ=255)
                                     gCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCt                                                       <  2:836583/93‑1 (MQ=255)
                                     gCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCt                                                       <  2:3852506/93‑1 (MQ=255)
                                     gCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCt                                                       >  2:1484314/1‑93 (MQ=255)
                                     gCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCt                                                       <  1:2117709/93‑1 (MQ=255)
                                       aGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTc                                                     >  2:2805389/1‑93 (MQ=255)
                                       aGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTc                                                     <  2:3502500/93‑1 (MQ=255)
                                        gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                    >  2:7249461/1‑93 (MQ=255)
                                        gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                    >  1:5996488/1‑93 (MQ=255)
                                         aTACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTg                                                   <  2:5514030/93‑1 (MQ=255)
                                            cGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGtt                                                <  1:4753357/93‑1 (MQ=255)
                                             gtgtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGttt                                               <  2:4168737/93‑1 (MQ=255)
                                             gtgtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGttt                                               <  2:3100752/93‑1 (MQ=255)
                                              tgtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTa                                              <  2:6576048/93‑1 (MQ=255)
                                                 aCGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCg                                           >  1:1384164/1‑93 (MQ=255)
                                                 aCGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCg                                           <  2:3732219/93‑1 (MQ=255)
                                                   gAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAt                                         >  1:7278829/1‑93 (MQ=255)
                                                    aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                        <  2:5209570/93‑1 (MQ=255)
                                                    aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                        <  1:6535295/93‑1 (MQ=255)
                                                    aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                        <  1:1283056/93‑1 (MQ=255)
                                                       tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTg                                     >  1:7473360/1‑93 (MQ=255)
                                                       tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCCCTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTg                                     >  1:250336/1‑93 (MQ=255)
                                                       tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTg                                     <  1:5803095/93‑1 (MQ=255)
                                                        ggCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGt                                    <  1:3178561/93‑1 (MQ=255)
                                                        ggCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGt                                    <  2:770470/93‑1 (MQ=255)
                                                          cTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTc                                  <  1:1544507/93‑1 (MQ=255)
                                                           tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                                 >  1:6645230/1‑93 (MQ=255)
                                                           tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                                 >  1:4160189/1‑93 (MQ=255)
                                                            ttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGGGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAc                                <  1:3978005/93‑1 (MQ=255)
                                                               aCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGc                             >  2:1638381/1‑93 (MQ=255)
                                                                  gCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAac                          >  2:5589731/1‑93 (MQ=255)
                                                                   ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                         >  1:1643869/1‑93 (MQ=255)
                                                                    cccACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacca                        <  1:3418382/93‑1 (MQ=255)
                                                                    cccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacca                        >  1:6190176/1‑93 (MQ=255)
                                                                    cccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacca                        >  1:1584382/1‑93 (MQ=255)
                                                                     ccACTCTTCCGGCAGGATGCCGCACTGGGAGGGGTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                       >  1:4650227/1‑93 (MQ=255)
                                                                     ccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                       >  1:2895955/1‑93 (MQ=255)
                                                                     ccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                       >  1:5706661/1‑93 (MQ=255)
                                                                     ccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                       >  1:3922443/1‑93 (MQ=255)
                                                                      cACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                      >  1:2220308/1‑93 (MQ=255)
                                                                      cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                      <  2:4190954/93‑1 (MQ=255)
                                                                       aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                     <  2:3656345/93‑1 (MQ=255)
                                                                       aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                     >  2:7524919/1‑93 (MQ=255)
                                                                        ctctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccac                    >  1:5230340/1‑93 (MQ=255)
                                                                        ctctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccac                    >  1:5088897/1‑93 (MQ=255)
                                                                         tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                   <  2:6802267/93‑1 (MQ=255)
                                                                         tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                   >  2:7351658/1‑93 (MQ=255)
                                                                          ctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCg                  <  2:2446366/93‑1 (MQ=255)
                                                                               ggCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTAcc             >  2:4726991/1‑93 (MQ=255)
                                                                                gCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCt            >  1:2434717/1‑93 (MQ=255)
                                                                                gCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCt            <  1:1054176/93‑1 (MQ=255)
                                                                                  aGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgc          <  2:3804141/93‑1 (MQ=255)
                                                                                   ggATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcg         >  1:7391204/1‑93 (MQ=255)
                                                                                    gATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcgc        >  2:2232376/1‑93 (MQ=255)
                                                                                      tGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccg      >  2:6023903/1‑93 (MQ=255)
                                                                                       gCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccgc     <  1:5303640/93‑1 (MQ=255)
                                                                                       gCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccgc     >  1:3830245/1‑93 (MQ=255)
                                                                                           |                                                                                           
AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT  >  NC_000913/3312015‑3312197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  >  NC_000913/3312013‑3312199
                                                                                             |                                                                                             
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGC                                                                                               >  SRR4302542.2479469/1‑94 (MQ=60)
  AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTAC                                                                                             >  SRR4302542.6513979/1‑94 (MQ=60)
  AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCAC                                                                                             <  SRR4302542.5916669/94‑1 (MQ=60)
  AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCAC                                                                                             >  SRR4302542.4132117/1‑94 (MQ=60)
   GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACT                                                                                            <  SRR4302542.5242423/94‑1 (MQ=60)
    CGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTG                                                                                           <  SRR4302542.2850812/94‑1 (MQ=60)
    CGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTG                                                                                           <  SRR4302542.2738602/94‑1 (MQ=60)
    CGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTG                                                                                           <  SRR4302542.6362395/94‑1 (MQ=60)
     GTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGG                                                                                          <  SRR4302542.397131/94‑1 (MQ=60)
     GTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGG                                                                                          >  SRR4302542.2139730/1‑94 (MQ=60)
      TCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGG                                                                                         <  SRR4302542.2924203/94‑1 (MQ=60)
       CCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGA                                                                                        >  SRR4302542.7066354/1‑94 (MQ=60)
       CCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGA                                                                                        >  SRR4302542.7352907/1‑94 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCCCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       <  SRR4302542.7541759/94‑1 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       <  SRR4302542.3480456/94‑1 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGA                                                                                      >  SRR4302542.423467/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGA                                                                                      >  SRR4302542.7306515/1‑94 (MQ=60)
          GCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAG                                                                                     >  SRR4302542.5857024/1‑94 (MQ=60)
          GCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAG                                                                                     <  SRR4302542.1136963/94‑1 (MQ=60)
           CAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGT                                                                                    <  SRR4302542.7388776/94‑1 (MQ=60)
            AGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTT                                                                                   <  SRR4302542.2244867/94‑1 (MQ=60)
            AGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTT                                                                                   >  SRR4302542.6400711/1‑94 (MQ=60)
            AGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTT                                                                                   <  SRR4302542.1765877/94‑1 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTC                                                                                  <  SRR4302542.5263887/94‑1 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTC                                                                                  >  SRR4302542.6349176/1‑94 (MQ=60)
               TCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGT                                                                                <  SRR4302542.1451490/94‑1 (MQ=60)
                CATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTT                                                                               >  SRR4302542.1588251/1‑94 (MQ=60)
                CATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTT                                                                               >  SRR4302542.4396873/1‑94 (MQ=60)
                 ATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTC                                                                              >  SRR4302542.4475329/1‑94 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                            <  SRR4302542.7217975/94‑1 (MQ=60)
                    GATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTA                                                                           <  SRR4302542.7282228/94‑1 (MQ=60)
                     ATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAA                                                                          >  SRR4302542.1898970/1‑94 (MQ=60)
                     ATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAA                                                                          <  SRR4302542.7080355/94‑1 (MQ=60)
                     ATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAA                                                                          <  SRR4302542.344217/94‑1 (MQ=60)
                      TACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAAC                                                                         <  SRR4302542.4368555/94‑1 (MQ=60)
                      TACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAC                                                                         <  SRR4302542.1795359/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302542.1712446/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302542.3703998/94‑1 (MQ=60)
                        CCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGC                                                                       <  SRR4302542.5581568/94‑1 (MQ=60)
                        CCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGC                                                                       <  SRR4302542.6735617/94‑1 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCG                                                                      >  SRR4302542.5808015/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCG                                                                      >  SRR4302542.6096550/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      >  SRR4302542.4041373/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      >  SRR4302542.3541740/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      <  SRR4302542.1589734/94‑1 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      >  SRR4302542.2539689/1‑94 (MQ=60)
                           GTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGAT                                                                    <  SRR4302542.2526424/94‑1 (MQ=60)
                           GTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAT                                                                    >  SRR4302542.2944779/1‑94 (MQ=60)
                           GTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAT                                                                    >  SRR4302542.7296621/1‑94 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGTGAGAGTTCGCTCTTAACGCGAACC                                                                  >  SRR4302542.5503442/1‑94 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                  <  SRR4302542.3039963/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 <  SRR4302542.4802236/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 >  SRR4302542.1964718/1‑94 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 <  SRR4302542.5621330/94‑1 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              <  SRR4302542.6822688/94‑1 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              <  SRR4302542.1155819/94‑1 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             >  SRR4302542.1123950/1‑94 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             <  SRR4302542.302256/94‑1 (MQ=60)
                                   TTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCA                                                            <  SRR4302542.2052212/94‑1 (MQ=60)
                                   TTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCA                                                            <  SRR4302542.3323278/94‑1 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCT                                                         <  SRR4302542.228913/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        >  SRR4302542.4699080/1‑94 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        <  SRR4302542.7223755/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        <  SRR4302542.2135984/94‑1 (MQ=60)
                                        CAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTC                                                       >  SRR4302542.1167096/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      >  SRR4302542.1655160/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      >  SRR4302542.6708440/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302542.4631775/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302542.6300446/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      >  SRR4302542.6054565/1‑94 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     <  SRR4302542.6648674/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    <  SRR4302542.2284304/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    >  SRR4302542.396067/1‑94 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    >  SRR4302542.428708/1‑94 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    >  SRR4302542.1367831/1‑94 (MQ=60)
                                             ACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTT                                                  <  SRR4302542.3604263/94‑1 (MQ=60)
                                              CGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTT                                                 <  SRR4302542.4406754/94‑1 (MQ=60)
                                              CGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTT                                                 <  SRR4302542.4799661/94‑1 (MQ=60)
                                              CGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTT                                                 <  SRR4302542.5820389/94‑1 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               >  SRR4302542.1421451/1‑94 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               >  SRR4302542.1327446/1‑94 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               <  SRR4302542.29256/94‑1 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               >  SRR4302542.6346515/1‑94 (MQ=60)
                                                 GTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                              >  SRR4302542.5701418/1‑94 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                             <  SRR4302542.2657433/94‑1 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                             <  SRR4302542.4674233/94‑1 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                             >  SRR4302542.1396123/1‑94 (MQ=60)
                                                    CGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAT                                           >  SRR4302542.6543897/1‑94 (MQ=60)
                                                    CGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAT                                           >  SRR4302542.7350215/1‑94 (MQ=60)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                          >  SRR4302542.5936317/1‑94 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         <  SRR4302542.3459707/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         >  SRR4302542.5632818/1‑94 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         <  SRR4302542.6599706/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         <  SRR4302542.1294100/94‑1 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302542.7546592/1‑94 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCCCTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302542.252474/1‑94 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302542.3100148/1‑94 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302542.5323794/1‑94 (MQ=60)
                                                         TGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGT                                      <  SRR4302542.5859410/94‑1 (MQ=60)
                                                          GGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTT                                     <  SRR4302542.3208684/94‑1 (MQ=60)
                                                          GGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTT                                     >  SRR4302542.2207485/1‑94 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                    <  SRR4302542.540144/94‑1 (MQ=60)
                                                            CTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCA                                   >  SRR4302542.4201159/1‑94 (MQ=60)
                                                            CTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCA                                   >  SRR4302542.6710725/1‑94 (MQ=60)
                                                            CTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCA                                   <  SRR4302542.1557834/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGGGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                 <  SRR4302542.4017255/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                 <  SRR4302542.7486732/94‑1 (MQ=60)
                                                               TCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTG                                <  SRR4302542.1439931/94‑1 (MQ=60)
                                                                 ACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              >  SRR4302542.2093536/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            >  SRR4302542.4870094/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           <  SRR4302542.1474527/94‑1 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302542.6152046/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302542.1658073/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                          >  SRR4302542.1880927/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                          >  SRR4302542.6250366/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                          >  SRR4302542.1598058/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         <  SRR4302542.3450410/94‑1 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGGGGTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.4695689/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.2923751/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.6352606/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.3961148/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.2326047/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302542.5762055/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        >  SRR4302542.2239408/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        <  SRR4302542.5335829/94‑1 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        >  SRR4302542.3748778/1‑94 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       <  SRR4302542.151107/94‑1 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       >  SRR4302542.2064504/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                      <  SRR4302542.440735/94‑1 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                      >  SRR4302542.5281203/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                      >  SRR4302542.5138360/1‑94 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                     >  SRR4302542.1763122/1‑94 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                     <  SRR4302542.3582663/94‑1 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                     >  SRR4302542.4777485/1‑94 (MQ=60)
                                                                           TCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCG                    <  SRR4302542.2798818/94‑1 (MQ=60)
                                                                           TCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCG                    <  SRR4302542.6907072/94‑1 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   >  SRR4302542.2313902/1‑94 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   >  SRR4302542.2486872/1‑94 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   >  SRR4302542.3873979/1‑94 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   >  SRR4302542.6928756/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT              >  SRR4302542.2458408/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT              >  SRR4302542.3956748/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT              >  SRR4302542.587227/1‑94 (MQ=60)
                                                                                  GCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTG             <  SRR4302542.1607324/94‑1 (MQ=60)
                                                                                  GCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTG             <  SRR4302542.1063238/94‑1 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC            <  SRR4302542.1964120/94‑1 (MQ=60)
                                                                                    AGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           <  SRR4302542.5385809/94‑1 (MQ=60)
                                                                                    AGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           >  SRR4302542.1121856/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           <  SRR4302542.6798824/94‑1 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           <  SRR4302542.3845360/94‑1 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           >  SRR4302542.7463551/1‑94 (MQ=60)
                                                                                     GGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGC          >  SRR4302542.5603133/1‑94 (MQ=60)
                                                                                      GATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCC         >  SRR4302542.1923424/1‑94 (MQ=60)
                                                                                        TGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGC       >  SRR4302542.3868131/1‑94 (MQ=60)
                                                                                         GCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT      <  SRR4302542.5355166/94‑1 (MQ=60)
                                                                                          CCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTT     >  SRR4302542.5793148/1‑94 (MQ=60)
                                                                                             CACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  <  SRR4302542.6250497/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  >  NC_000913/3312013‑3312199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑