Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,317,651	G→A	P535L (CCC→CTC)	b3173 ←	putative alkaline phosphatase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,317,651	0	G	A	83.0%	687.5 / 97.5	265	P535L (CCC→CTC)	b3173	putative alkaline phosphatase I
Reads supporting (aligned to +/- strand): ref base G (20/25); new base A (100/120); total (120/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 280 total aligned reads displayed.

TTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTT  >  NC_000913/3317561‑3317742

ttCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAggggtgagg                                                                                           >  2:6434291/1‑93 (MQ=255)

nncctcctCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAggggtgggg                                                                                           <  2:130333/91‑1 (MQ=255)

  cctcctCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGtt                                                                                         >  1:3144371/1‑93 (MQ=255)

  cctcctCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGtt                                                                                         <  2:1729247/93‑1 (MQ=255)

   ctcctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTa                                                                                        <  1:6669626/93‑1 (MQ=255)

   ctcctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTa                                                                                        >  1:813715/1‑93 (MQ=255)

   ctcctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTa                                                                                        >  1:2572325/1‑93 (MQ=255)

    tcctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTAc                                                                                       <  2:792210/93‑1 (MQ=255)

     cctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTAcc                                                                                      >  1:1116345/1‑93 (MQ=255)

     cctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTAcc                                                                                      <  2:927336/93‑1 (MQ=255)

     cctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTAcc                                                                                      >  1:5094607/1‑93 (MQ=255)

       tctGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCtt                                                                                    <  1:7201435/93‑1 (MQ=255)

        ctGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTg                                                                                   >  2:7090967/1‑93 (MQ=255)

        ctGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTg                                                                                   <  1:3008443/93‑1 (MQ=255)

         tGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGc                                                                                  <  2:5310302/93‑1 (MQ=255)

          gCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCt                                                                                 >  2:7148848/1‑93 (MQ=255)

          gCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCt                                                                                 >  1:1891889/1‑93 (MQ=255)

            cGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTa                                                                               >  2:279982/1‑93 (MQ=255)

             gCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTAt                                                                              >  2:455664/1‑93 (MQ=255)

              cTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATc                                                                             <  1:7126247/93‑1 (MQ=255)

               ttCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATcc                                                                            <  2:1522353/93‑1 (MQ=255)

               ttCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATcc                                                                            >  2:3905536/1‑93 (MQ=255)

                tCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCct                                                                           <  2:3489053/93‑1 (MQ=255)

                 cAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCctc                                                                          <  1:4079234/93‑1 (MQ=255)

                   ggCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTg                                                                        >  2:4000904/1‑93 (MQ=255)

                    gCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGt                                                                       >  2:601362/1‑93 (MQ=255)

                     cGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTa                                                                      <  1:5219271/93‑1 (MQ=255)

                       tGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTaaa                                                                    >  2:6655574/1‑93 (MQ=255)

                       tGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTaaa                                                                    <  1:4330170/93‑1 (MQ=255)

                        gCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGTTGAGGTTACCTTGCTTATCCTCTGTAAAc                                                                   <  2:1331579/93‑1 (MQ=255)

                        gCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAAc                                                                   <  2:5420842/93‑1 (MQ=255)

                          cGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTc                                                                 >  1:1051294/1‑93 (MQ=255)

                            cAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCt                                                               <  2:4798250/93‑1 (MQ=255)

                             aGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCtt                                                              <  2:3298332/93‑1 (MQ=255)

                             aGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCtt                                                              <  2:73933/93‑1 (MQ=255)

                             aGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCtt                                                              <  1:1483849/93‑1 (MQ=255)

                              ggTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCttt                                                             <  2:6815494/93‑1 (MQ=255)

                              ggTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCttt                                                             >  2:1086200/1‑93 (MQ=255)

                                tGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCt                                                           >  2:2146247/1‑93 (MQ=255)

                                 ggAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTg                                                          <  2:1137539/93‑1 (MQ=255)

                                 ggAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTg                                                          <  2:945285/93‑1 (MQ=255)

                                 ggAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTg                                                          <  1:2312770/93‑1 (MQ=255)

                                  gagaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGa                                                         <  1:1956747/93‑1 (MQ=255)

                                  gagaATCTGGAAAACAAAGGCCAGGAATTCCACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCtta                                                         >  1:6200555/1‑91 (MQ=255)

                                     aaTCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCa                                                      <  1:4939342/93‑1 (MQ=255)

                                      aTCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCAt                                                     >  1:5562371/1‑93 (MQ=255)

                                        cTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATca                                                   <  1:3528021/93‑1 (MQ=255)

                                        cTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATca                                                   <  1:3279330/93‑1 (MQ=255)

                                          ggAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATcaca                                                 >  2:2705958/1‑93 (MQ=255)

                                           gAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACAt                                                <  1:4626207/93‑1 (MQ=255)

                                           gAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACAt                                                <  1:5058271/93‑1 (MQ=255)

                                            aaaaCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATa                                               <  1:1653783/93‑1 (MQ=255)

                                            aaaaCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATa                                               <  2:7304704/93‑1 (MQ=255)

                                             aaaCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATaa                                              <  1:3392845/93‑1 (MQ=255)

                                             aaaCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATaa                                              >  1:5306585/1‑93 (MQ=255)

                                              aaCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCGGAGCATCACATAAt                                             <  2:5197455/93‑1 (MQ=255)

                                               aCAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATc                                            <  1:5908435/93‑1 (MQ=255)

                                                cAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCg                                           >  2:571279/1‑93 (MQ=255)

                                                  aaGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGaa                                         >  2:818965/1‑93 (MQ=255)

                                                  aaGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGaa                                         >  2:5751615/1‑93 (MQ=255)

                                                    ggCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGaaaa                                       <  2:2943738/93‑1 (MQ=255)

                                                    ggCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGaaaa                                       <  2:586033/93‑1 (MQ=255)

                                                     gCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAc                                      <  1:365318/93‑1 (MQ=255)

                                                      ccAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAca                                     <  1:5852961/93‑1 (MQ=255)

                                                      ccAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAca                                     <  2:1575111/93‑1 (MQ=255)

                                                       cAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAcat                                    <  1:445361/93‑1 (MQ=255)

                                                       cAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAcat                                    >  2:656828/1‑93 (MQ=255)

                                                        aGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAAcatc                                   <  1:1698269/93‑1 (MQ=255)

                                                           aaTTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATg                                >  2:5042650/1‑93 (MQ=255)

                                                            aTTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATgg                               <  2:3811054/93‑1 (MQ=255)

                                                            aTTCGACATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATgg                               >  1:3824188/1‑93 (MQ=255)

                                                             ttCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGt                              >  1:638462/1‑93 (MQ=255)

                                                              tCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGtt                             <  1:6650727/93‑1 (MQ=255)

                                                              tCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGtt                             <  1:7349363/93‑1 (MQ=255)

                                                                 aCATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGc                          >  2:6023359/1‑93 (MQ=255)

                                                                  cATCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCa                         <  2:4271618/93‑1 (MQ=255)

                                                                   aTCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCAt                        >  1:299374/1‑93 (MQ=255)

                                                                   aTCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCAt                        <  2:247623/93‑1 (MQ=255)

                                                                   aTCAACCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCAt                        <  1:4307835/93‑1 (MQ=255)

                                                                      aaCCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCca                     >  1:4141225/1‑93 (MQ=255)

                                                                      aaCCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCca                     >  1:1234073/1‑93 (MQ=255)

                                                                       aCCCTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCcac                    <  2:2363084/93‑1 (MQ=255)

                                                                        cccTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCcaca                   >  2:6332761/1‑93 (MQ=255)

                                                                        cccTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCcaca                   >  1:2030823/1‑93 (MQ=255)

                                                                        cccTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCcaca                   <  2:719066/93‑1 (MQ=255)

                                                                          cTGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAc                 >  2:30614/1‑93 (MQ=255)

                                                                           tGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAcc                <  1:958356/93‑1 (MQ=255)

                                                                           tGCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAcc                >  2:4093988/1‑93 (MQ=255)

                                                                            gCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAccc               <  2:2240214/93‑1 (MQ=255)

                                                                            gCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAccc               <  1:2247645/93‑1 (MQ=255)

                                                                            gCCCTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAccc               <  2:6103105/93‑1 (MQ=255)

                                                                              ccTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGt             <  2:4512226/93‑1 (MQ=255)

                                                                               cTTCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTg            >  1:1077830/1‑93 (MQ=255)

                                                                                ttCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGa           >  1:6872119/1‑93 (MQ=255)

                                                                                 tCAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAg          >  2:7497531/1‑93 (MQ=255)

                                                                                  cAGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt         <  1:9013/93‑1 (MQ=255)

                                                                                   aGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGtc        <  1:2083992/93‑1 (MQ=255)

                                                                                   aGGGGTGAGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGtc        <  2:6775668/93‑1 (MQ=255)

                                                                                       gtgaggtTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCt    <  1:2716658/93‑1 (MQ=255)

                                                                                         ggggtTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCCTCCACAACCCGTGAGTCTCCttt  >  2:6975731/1‑93 (MQ=255)

TTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTT  >  NC_000913/3317561‑3317742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTA  >  NC_000913/3317559‑3317743

CTTTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AG                                                                                             <  SRR4302542.1912668/94‑1 (MQ=60)

CTCTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AG                                                                                             <  SRR4302542.4632323/94‑1 (MQ=60)

 TTTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGG                                                                                            >  SRR4302542.3352383/1‑94 (MQ=60)

  TTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGT                                                                                           >  SRR4302542.2979975/1‑94 (MQ=60)

   TCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGAGGGT                                                                                           >  SRR4302542.1219206/1‑94 (MQ=60)

   TCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTT                                                                                          >  SRR4302542.935742/1‑94 (MQ=60)

   TCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTT                                                                                          >  SRR4302542.3174243/1‑94 (MQ=60)

    CCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTA                                                                                         >  SRR4302542.2597247/1‑94 (MQ=60)

    CCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTA                                                                                         >  SRR4302542.6116143/1‑94 (MQ=60)

    CCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTA                                                                                         >  SRR4302542.820758/1‑94 (MQ=60)

     CTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTAC                                                                                        <  SRR4302542.6735341/94‑1 (MQ=60)

      TCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACC                                                                                       >  SRR4302542.5144110/1‑94 (MQ=60)

      TCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACC                                                                                       >  SRR4302542.1125885/1‑94 (MQ=60)

      TCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACC                                                                                       <  SRR4302542.4364910/94‑1 (MQ=60)

       CCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCT                                                                                      <  SRR4302542.2712817/94‑1 (MQ=60)

       CCTCTGCCGCTTCAGGCGTGCCGCAGGGGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCT                                                                                      >  SRR4302542.3200663/1‑94 (MQ=60)

         TCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTG                                                                                    <  SRR4302542.7272112/94‑1 (MQ=60)

          CTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGC                                                                                   <  SRR4302542.3037206/94‑1 (MQ=60)

           TGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCT                                                                                  >  SRR4302542.1908262/1‑94 (MQ=60)

             CCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTA                                                                                >  SRR4302542.4555279/1‑94 (MQ=60)

               GCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATC                                                                              <  SRR4302542.7381600/94‑1 (MQ=60)

               GCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATC                                                                              >  SRR4302542.5163553/1‑94 (MQ=60)

                CTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCC                                                                             <  SRR4302542.4373841/94‑1 (MQ=60)

                CTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCC                                                                             <  SRR4302542.7196185/94‑1 (MQ=60)

                 TTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCT                                                                            >  SRR4302542.1102481/1‑94 (MQ=60)

                   CAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCT                                                                          <  SRR4302542.4135583/94‑1 (MQ=60)

                   CAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCT                                                                          <  SRR4302542.4119437/94‑1 (MQ=60)

                    AGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTG                                                                         >  SRR4302542.4970725/1‑94 (MQ=60)

                     GGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGT                                                                        <  SRR4302542.3545326/94‑1 (MQ=60)

                     GGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGT                                                                        >  SRR4302542.5671455/1‑94 (MQ=60)

                      GCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTA                                                                       <  SRR4302542.4338566/94‑1 (MQ=60)

                       CGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAA                                                                      >  SRR4302542.3372328/1‑94 (MQ=60)

                       CGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAA                                                                      <  SRR4302542.5270027/94‑1 (MQ=60)

                         TGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAAC                                                                    <  SRR4302542.4372732/94‑1 (MQ=60)

                         TGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAAC                                                                    >  SRR4302542.304173/1‑94 (MQ=60)

                          GCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACT                                                                   <  SRR4302542.1394751/94‑1 (MQ=60)

                          GCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACT                                                                   <  SRR4302542.545503/94‑1 (MQ=60)

                           CCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTC                                                                  <  SRR4302542.1062544/94‑1 (MQ=60)

                           CCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTC                                                                  >  SRR4302542.1060337/1‑94 (MQ=60)

                             GCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCT                                                                >  SRR4302542.6086828/1‑94 (MQ=60)

                              CAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTT                                                               <  SRR4302542.2050201/94‑1 (MQ=60)

                               AGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTT                                                              <  SRR4302542.6845279/94‑1 (MQ=60)

                               AGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTT                                                              >  SRR4302542.7008315/1‑94 (MQ=60)

                               AGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTT                                                              <  SRR4302542.1496656/94‑1 (MQ=60)

                                 GTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCT                                                            <  SRR4302542.1792860/94‑1 (MQ=60)

                                 GTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCT                                                            >  SRR4302542.7478927/1‑94 (MQ=60)

                                  TGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTG                                                           >  SRR4302542.2209278/1‑94 (MQ=60)

                                   GGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGA                                                          >  SRR4302542.1151783/1‑94 (MQ=60)

                                   GGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGA                                                          >  SRR4302542.5328551/1‑94 (MQ=60)

                                   GGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGA                                                          >  SRR4302542.4457477/1‑94 (MQ=60)

                                   GGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGA                                                          <  SRR4302542.2332624/94‑1 (MQ=60)

                                   GGAGAATCTGGAAAACAAAGGCCAGGAATTCCACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTTA                                                          >  SRR4302542.6261196/1‑94 (MQ=60)

                                    GAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAG                                                         <  SRR4302542.2586425/94‑1 (MQ=60)

                                    GAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAG                                                         <  SRR4302542.1973667/94‑1 (MQ=60)

                                      GAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCA                                                       <  SRR4302542.5048807/94‑1 (MQ=60)

                                      GAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCA                                                       >  SRR4302542.271997/1‑94 (MQ=60)

                                       AATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCAT                                                      >  SRR4302542.6724009/1‑94 (MQ=60)

                                       AATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCAT                                                      >  SRR4302542.5616416/1‑94 (MQ=60)

                                       AATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCAT                                                      <  SRR4302542.4987346/94‑1 (MQ=60)

                                       AATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCAT                                                      <  SRR4302542.2922787/94‑1 (MQ=60)

                                        ATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATC                                                     <  SRR4302542.2543031/94‑1 (MQ=60)

                                          CTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCAC                                                   <  SRR4302542.3560994/94‑1 (MQ=60)

                                          CTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCAC                                                   <  SRR4302542.3310270/94‑1 (MQ=60)

                                             GAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATA                                                <  SRR4302542.4671404/94‑1 (MQ=60)

                                             GAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATA                                                <  SRR4302542.5107388/94‑1 (MQ=60)

                                              AAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAA                                               >  SRR4302542.5358140/1‑94 (MQ=60)

                                              AAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAA                                               <  SRR4302542.1668068/94‑1 (MQ=60)

                                               AAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAAT                                              <  SRR4302542.3424686/94‑1 (MQ=60)

                                               AAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAAT                                              >  SRR4302542.2020618/1‑94 (MQ=60)

                                                AACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATC                                             <  SRR4302542.2312092/94‑1 (MQ=60)

                                                 ACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCG                                            <  SRR4302542.5965693/94‑1 (MQ=60)

                                                 ACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCG                                            <  SRR4302542.1688301/94‑1 (MQ=60)

                                                  CAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGA                                           >  SRR4302542.3858248/1‑94 (MQ=60)

                                                  CAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGA                                           <  SRR4302542.233160/94‑1 (MQ=60)

                                                     AGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAA                                        >  SRR4302542.3072082/1‑94 (MQ=60)

                                                       GCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACA                                      <  SRR4302542.368491/94‑1 (MQ=60)

                                                        CCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACAT                                     <  SRR4302542.6731704/94‑1 (MQ=60)

                                                        CCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACAT                                     <  SRR4302542.5909725/94‑1 (MQ=60)

                                                         CAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATC                                    >  SRR4302542.7246980/1‑94 (MQ=60)

                                                         CAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATC                                    <  SRR4302542.3431741/94‑1 (MQ=60)

                                                         CAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATC                                    <  SRR4302542.449212/94‑1 (MQ=60)

                                                          AGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCA                                   <  SRR4302542.5532909/94‑1 (MQ=60)

                                                          AGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCA                                   <  SRR4302542.1988878/94‑1 (MQ=60)

                                                          AGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCA                                   >  SRR4302542.1804668/1‑94 (MQ=60)

                                                          AGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCA                                   >  SRR4302542.7422562/1‑94 (MQ=60)

                                                          AGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCA                                   <  SRR4302542.1712983/94‑1 (MQ=60)

                                                           GTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCAT                                  <  SRR4302542.1507650/94‑1 (MQ=60)

                                                           GTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCAT                                  <  SRR4302542.5858015/94‑1 (MQ=60)

                                                            TAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATG                                 >  SRR4302542.1933353/1‑94 (MQ=60)

                                                            TAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATG                                 <  SRR4302542.3785494/94‑1 (MQ=60)

                                                            TAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATG                                 >  SRR4302542.4949082/1‑94 (MQ=60)

                                                            TAATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATG                                 <  SRR4302542.3929935/94‑1 (MQ=60)

                                                             AATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGG                                >  SRR4302542.1527408/1‑94 (MQ=60)

                                                             AATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGG                                >  SRR4302542.3862015/1‑94 (MQ=60)

                                                              ATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGT                               >  SRR4302542.643956/1‑94 (MQ=60)

                                                              ATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGT                               >  SRR4302542.7131327/1‑94 (MQ=60)

                                                              ATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGT                               >  SRR4302542.4599339/1‑94 (MQ=60)

                                                              ATTCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGT                               <  SRR4302542.4184730/94‑1 (MQ=60)

                                                                TCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTT                             <  SRR4302542.7421339/94‑1 (MQ=60)

                                                                TCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTT                             <  SRR4302542.6716268/94‑1 (MQ=60)

                                                                TCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTT                             <  SRR4302542.206229/94‑1 (MQ=60)

                                                                TCGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTT                             <  SRR4302542.628277/94‑1 (MQ=60)

                                                                 CGACATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTG                            <  SRR4302542.3659349/94‑1 (MQ=60)

                                                                    CATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCAT                         >  SRR4302542.954696/1‑94 (MQ=60)

                                                                    CATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCAT                         >  SRR4302542.301982/1‑94 (MQ=60)

                                                                     ATCAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATC                        <  SRR4302542.4350182/94‑1 (MQ=60)

                                                                     ATCAACCCTGCCATTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATC                        >  SRR4302542.4718037/1‑94 (MQ=60)

                                                                       CAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCA                      >  SRR4302542.4182025/1‑94 (MQ=60)

                                                                       CAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCA                      >  SRR4302542.3946075/1‑94 (MQ=60)

                                                                       CAACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCA                      >  SRR4302542.1244688/1‑94 (MQ=60)

                                                                        AACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCAC                     >  SRR4302542.195117/1‑94 (MQ=60)

                                                                        AACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCAC                     >  SRR4302542.303221/1‑94 (MQ=60)

                                                                         ACCCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACA                    >  SRR4302542.2048394/1‑94 (MQ=60)

                                                                           CCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAC                  <  SRR4302542.3230538/94‑1 (MQ=60)

                                                                           CCTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAAC                  <  SRR4302542.721705/94‑1 (MQ=60)

                                                                            CTGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACC                 >  SRR4302542.4120231/1‑94 (MQ=60)

                                                                             TGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCC                <  SRR4302542.966587/94‑1 (MQ=60)

                                                                             TGCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCC                >  SRR4302542.6458885/1‑94 (MQ=60)

                                                                              GCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCG               <  SRR4302542.2266974/94‑1 (MQ=60)

                                                                              GCCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCG               <  SRR4302542.7335559/94‑1 (MQ=60)

                                                                               CCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGT              >  SRR4302542.5236219/1‑94 (MQ=60)

                                                                               CCCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGT              >  SRR4302542.6330111/1‑94 (MQ=60)

                                                                                CCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTG             >  SRR4302542.1322435/1‑94 (MQ=60)

                                                                                CCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTG             >  SRR4302542.1087092/1‑94 (MQ=60)

                                                                                CCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTG             >  SRR4302542.887593/1‑94 (MQ=60)

                                                                                CCTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTG             >  SRR4302542.1087091/1‑94 (MQ=60)

                                                                                 CTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGA            >  SRR4302542.6939630/1‑94 (MQ=60)

                                                                                 CTTCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGA            >  SRR4302542.5119820/1‑94 (MQ=60)

                                                                                   TCAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGT          <  SRR4302542.6654766/94‑1 (MQ=60)

                                                                                    CAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTC         <  SRR4302542.5344447/94‑1 (MQ=60)

                                                                                    CAGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTC         <  SRR4302542.9088/94‑1 (MQ=60)

                                                                                     AGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCT        >  SRR4302542.5927078/1‑94 (MQ=60)

                                                                                     AGGGGTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCT        <  SRR4302542.2101987/94‑1 (MQ=60)

                                                                                         GTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTT    <  SRR4302542.2742857/94‑1 (MQ=60)

                                                                                         GTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTT    >  SRR4302542.2150014/1‑94 (MQ=60)

                                                                                         GTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTT    <  SRR4302542.5009120/94‑1 (MQ=60)

                                                                                         GTG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTT    >  SRR4302542.5153483/1‑94 (MQ=60)

                                                                                          TG‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTT   <  SRR4302542.1507609/94‑1 (MQ=60)

                                                                                           G‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTA  >  SRR4302542.3201707/1‑94 (MQ=60)

                                                                                           G‑AGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTA  <  SRR4302542.4911453/94‑1 (MQ=60)

CTTTCCTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTG‑GGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTA  >  NC_000913/3317559‑3317743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`