Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,319,717 A→G noncoding (83/87 nt) leuU ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,319,7170AG85.2% 754.4 / 122.0 325noncoding (83/87 nt)leuUtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (31/17);  new base G (175/102);  total (206/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 325 total aligned reads displayed.

GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806
                                                                                          |                                                                                         
    gagaTACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAgg                                                                                     <  2:5203186/93‑1 (MQ=255)
     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                    <  2:3233775/93‑1 (MQ=255)
     agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                    <  2:3565854/93‑1 (MQ=255)
      gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAc                                                                                   >  1:3622355/1‑93 (MQ=255)
       atacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACg                                                                                  <  2:568014/93‑1 (MQ=255)
         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACggg                                                                                >  1:6621241/1‑93 (MQ=255)
         acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACggg                                                                                >  2:5374332/1‑93 (MQ=255)
          cagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGa                                                                               >  2:2606229/1‑93 (MQ=255)
           agatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAc                                                                              >  1:4137854/1‑93 (MQ=255)
            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  1:7423207/1‑93 (MQ=255)
            gatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACt                                                                             >  2:2277965/1‑93 (MQ=255)
              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           <  1:6928968/93‑1 (MQ=255)
              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           >  1:6174704/1‑93 (MQ=255)
              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTg                                                                           >  2:678904/1‑93 (MQ=255)
              tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTg                                                                           >  1:5760875/1‑93 (MQ=255)
                ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGaa                                                                         >  1:7218077/1‑93 (MQ=255)
                  tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTAGTACCGAGGACGGGACTTGAAcc                                                                       <  2:7481972/93‑1 (MQ=255)
                      aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTa                                                                   >  2:3206300/1‑93 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  >  1:3068902/1‑93 (MQ=255)
                       aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTaa                                                                  <  2:6118660/93‑1 (MQ=255)
                          aTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGcc                                                               >  1:1002680/1‑93 (MQ=255)
                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  1:107829/93‑1 (MQ=255)
                           ttCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGccc                                                              <  2:4127488/93‑1 (MQ=255)
                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  1:776510/93‑1 (MQ=255)
                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  2:4429740/93‑1 (MQ=255)
                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  2:2803944/93‑1 (MQ=255)
                             ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTa                                                            <  1:2489910/93‑1 (MQ=255)
                              cTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAt                                                           <  2:1102954/93‑1 (MQ=255)
                              cTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTAt                                                           <  2:3470529/93‑1 (MQ=255)
                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          <  1:4761416/93‑1 (MQ=255)
                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          >  2:675710/1‑93 (MQ=255)
                               tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAtt                                                          >  1:6088243/1‑93 (MQ=255)
                                cAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                         <  2:5543745/93‑1 (MQ=255)
                                 aGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                        >  2:2520091/1‑93 (MQ=255)
                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      >  1:1864344/1‑93 (MQ=255)
                                   aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                      <  2:6838300/93‑1 (MQ=255)
                                    ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCa                                                     >  2:6324073/1‑93 (MQ=255)
                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                    >  1:267072/1‑93 (MQ=255)
                                     cAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                    >  2:3693730/1‑93 (MQ=255)
                                       ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                  >  1:3260372/1‑93 (MQ=255)
                                        gACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                 >  1:1730008/1‑93 (MQ=255)
                                        gACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                                                 <  2:6941721/93‑1 (MQ=255)
                                         aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                >  1:1988234/1‑93 (MQ=255)
                                         aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                >  1:7092048/1‑93 (MQ=255)
                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacca                                               <  1:6345037/93‑1 (MQ=255)
                                          ccAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAAGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacca                                               <  2:2935999/93‑1 (MQ=255)
                                            aaaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                             >  2:6393444/1‑93 (MQ=255)
                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  1:1916568/1‑93 (MQ=255)
                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  2:3044814/1‑93 (MQ=255)
                                             aaaCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                            >  2:472499/1‑93 (MQ=255)
                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          <  1:6696201/93‑1 (MQ=255)
                                               aCGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCa                                          >  1:1708712/1‑93 (MQ=255)
                                                 gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                        >  2:4080675/1‑93 (MQ=255)
                                                 gAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                        >  1:2307666/1‑93 (MQ=255)
                                                   aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt                                      <  1:3749678/93‑1 (MQ=255)
                                                    aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                     >  1:3540079/1‑93 (MQ=255)
                                                      aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                   >  1:6759482/1‑93 (MQ=255)
                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  2:1424593/1‑93 (MQ=255)
                                                       aGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                  >  1:5028565/1‑93 (MQ=255)
                                                        gACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgt                                 >  2:390735/1‑93 (MQ=255)
                                                         aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                <  2:7164868/93‑1 (MQ=255)
                                                          cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtgt                               >  1:1061445/1‑93 (MQ=255)
                                                           gCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTc                              >  2:1750028/1‑93 (MQ=255)
                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:2105141/1‑93 (MQ=255)
                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            <  2:6558361/93‑1 (MQ=255)
                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:2557175/1‑93 (MQ=255)
                                                             ttttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTa                            >  1:5653800/1‑93 (MQ=255)
                                                              tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                           >  1:4593391/1‑93 (MQ=255)
                                                                  aGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                       <  2:729440/93‑1 (MQ=255)
                                                                   gCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                      <  2:6220117/93‑1 (MQ=255)
                                                                    cGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTc                     <  2:3305950/93‑1 (MQ=255)
                                                                     gTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                    >  1:821472/1‑93 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   <  2:7035548/93‑1 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:489807/1‑93 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:4569636/1‑93 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  2:6489150/1‑93 (MQ=255)
                                                                      tctcTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcca                   >  1:593814/1‑93 (MQ=255)
                                                                       ctctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  >  2:1906957/1‑93 (MQ=255)
                                                                       ctctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  <  1:3882726/93‑1 (MQ=255)
                                                                       ctctTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                  <  1:2182714/93‑1 (MQ=255)
                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:4242344/1‑93 (MQ=255)
                                                                        tctTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccacc                 >  1:525132/1‑93 (MQ=255)
                                                                          ttttCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCcaccac               >  1:1599575/1‑93 (MQ=255)
                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             <  2:1959633/93‑1 (MQ=255)
                                                                            ttCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt             >  1:1106666/1‑93 (MQ=255)
                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           <  2:6902387/93‑1 (MQ=255)
                                                                              cTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  2:1258844/1‑93 (MQ=255)
                                                                              cTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCg           >  1:7339762/1‑93 (MQ=255)
                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         >  2:5899767/1‑93 (MQ=255)
                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         <  1:1394014/93‑1 (MQ=255)
                                                                                ggAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc         >  1:3374639/1‑93 (MQ=255)
                                                                                 gAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCa        <  2:7392473/93‑1 (MQ=255)
                                                                                 gAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCa        <  1:1524752/93‑1 (MQ=255)
                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:6506090/1‑93 (MQ=255)
                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  1:571937/1‑93 (MQ=255)
                                                                                  aaTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACAACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       <  2:6007163/93‑1 (MQ=255)
                                                                                  aaTTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAc       >  2:836231/1‑93 (MQ=255)
                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  1:3135754/1‑93 (MQ=255)
                                                                                   aTTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACg      >  2:4355388/1‑93 (MQ=255)
                                                                                    tttGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACgg     >  1:519304/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA  >  NC_000913/3319627‑3319806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC  >  NC_000913/3319624‑3319807
                                                                                             |                                                                                          
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTG                                                                                            <  SRR4302542.4086981/94‑1 (MQ=60)
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTG                                                                                            <  SRR4302542.4620532/94‑1 (MQ=60)
  TGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGTCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCC                                                                                          >  SRR4302542.2653867/1‑94 (MQ=60)
  TGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCC                                                                                          >  SRR4302542.4508356/1‑94 (MQ=60)
   GGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCG                                                                                         >  SRR4302542.7316620/1‑94 (MQ=60)
     ACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAG                                                                                       <  SRR4302542.451429/94‑1 (MQ=60)
      CGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGG                                                                                      <  SRR4302542.1730325/94‑1 (MQ=60)
      CGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGG                                                                                      >  SRR4302542.3191571/1‑94 (MQ=60)
      CGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGG                                                                                      <  SRR4302542.1037857/94‑1 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                    >  SRR4302542.4640816/1‑94 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                    <  SRR4302542.7141663/94‑1 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                    <  SRR4302542.1970116/94‑1 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                    <  SRR4302542.1073006/94‑1 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGAC                                                                                    >  SRR4302542.6358259/1‑94 (MQ=60)
        AGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAC                                                                                    >  SRR4302542.3656100/1‑94 (MQ=60)
         GATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACG                                                                                   <  SRR4302542.6704051/94‑1 (MQ=60)
          ATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGG                                                                                  >  SRR4302542.1892753/1‑94 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGG                                                                                 >  SRR4302542.6460435/1‑94 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGG                                                                                 <  SRR4302542.1467389/94‑1 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGG                                                                                 >  SRR4302542.6686528/1‑94 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGG                                                                                 <  SRR4302542.2659739/94‑1 (MQ=60)
           TACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGG                                                                                 <  SRR4302542.6199091/94‑1 (MQ=60)
             CAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAC                                                                               >  SRR4302542.4178625/1‑94 (MQ=60)
             CAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGAC                                                                               >  SRR4302542.1699037/1‑94 (MQ=60)
              AGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACT                                                                              >  SRR4302542.7495843/1‑94 (MQ=60)
                ATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTG                                                                            >  SRR4302542.6234558/1‑94 (MQ=60)
                ATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTG                                                                            <  SRR4302542.7087094/94‑1 (MQ=60)
                ATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTG                                                                            >  SRR4302542.5816798/1‑94 (MQ=60)
                 TACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGA                                                                           <  SRR4302542.6996997/94‑1 (MQ=60)
                 TACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGA                                                                           >  SRR4302542.4264622/1‑94 (MQ=60)
                  ACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAA                                                                          >  SRR4302542.7288883/1‑94 (MQ=60)
                   CCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAC                                                                         >  SRR4302542.5165421/1‑94 (MQ=60)
                   CCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAAC                                                                         >  SRR4302542.5993485/1‑94 (MQ=60)
                    CTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACC                                                                        >  SRR4302542.5781537/1‑94 (MQ=60)
                    CTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACC                                                                        >  SRR4302542.571395/1‑94 (MQ=60)
                    CTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACC                                                                        <  SRR4302542.2162963/94‑1 (MQ=60)
                        CAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTA                                                                    >  SRR4302542.6811200/1‑94 (MQ=60)
                        CAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTA                                                                    >  SRR4302542.527433/1‑94 (MQ=60)
                        CAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTA                                                                    >  SRR4302542.5765562/1‑94 (MQ=60)
                         AACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAA                                                                   >  SRR4302542.3098156/1‑94 (MQ=60)
                          ACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAG                                                                  <  SRR4302542.2094327/94‑1 (MQ=60)
                            CATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCC                                                                >  SRR4302542.1011309/1‑94 (MQ=60)
                             ATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGGGGACGGGACTTGAACCCGTAAGCCC                                                               >  SRR4302542.2569868/1‑94 (MQ=60)
                             ATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCC                                                               >  SRR4302542.6820803/1‑94 (MQ=60)
                             ATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCC                                                               >  SRR4302542.3895472/1‑94 (MQ=60)
                             ATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCC                                                               <  SRR4302542.959500/94‑1 (MQ=60)
                              TTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCT                                                              <  SRR4302542.108712/94‑1 (MQ=60)
                                CCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAT                                                            <  SRR4302542.2514065/94‑1 (MQ=60)
                                CCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTAT                                                            <  SRR4302542.783219/94‑1 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           >  SRR4302542.192430/1‑94 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           >  SRR4302542.4025617/1‑94 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           <  SRR4302542.2836579/94‑1 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           >  SRR4302542.6147193/1‑94 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           >  SRR4302542.7506343/1‑94 (MQ=60)
                                 CTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATT                                                           <  SRR4302542.4551538/94‑1 (MQ=60)
                                  TCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTG                                                          <  SRR4302542.4807841/94‑1 (MQ=60)
                                   CAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGG                                                         >  SRR4302542.3690209/1‑94 (MQ=60)
                                   CAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGG                                                         <  SRR4302542.4172862/94‑1 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                        >  SRR4302542.2105266/1‑94 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                        >  SRR4302542.112456/1‑94 (MQ=60)
                                    AGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGG                                                        >  SRR4302542.365260/1‑94 (MQ=60)
                                     GACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGC                                                       >  SRR4302542.1880471/1‑94 (MQ=60)
                                       CCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAC                                                     >  SRR4302542.269344/1‑94 (MQ=60)
                                       CCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAC                                                     >  SRR4302542.5230156/1‑94 (MQ=60)
                                        CAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACT                                                    >  SRR4302542.6154194/1‑94 (MQ=60)
                                        CAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACT                                                    >  SRR4302542.2921818/1‑94 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                   >  SRR4302542.3291162/1‑94 (MQ=60)
                                         AGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTA                                                   >  SRR4302542.3766885/1‑94 (MQ=60)
                                          GGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTAC                                                  >  SRR4302542.1745022/1‑94 (MQ=60)
                                           GACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACC                                                 <  SRR4302542.735140/94‑1 (MQ=60)
                                           GACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACC                                                 >  SRR4302542.2003262/1‑94 (MQ=60)
                                           GACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACC                                                 >  SRR4302542.7161623/1‑94 (MQ=60)
                                           GACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACC                                                 >  SRR4302542.2005386/1‑94 (MQ=60)
                                             CCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCAC                                               <  SRR4302542.346689/94‑1 (MQ=60)
                                             CCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCAC                                               <  SRR4302542.823428/94‑1 (MQ=60)
                                             CCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCAC                                               <  SRR4302542.6407541/94‑1 (MQ=60)
                                              CAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTGTTGGGCACTACCACC                                              >  SRR4302542.6074102/1‑94 (MQ=60)
                                              CAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACC                                              >  SRR4302542.4783779/1‑94 (MQ=60)
                                               AAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCT                                             >  SRR4302542.1933130/1‑94 (MQ=60)
                                               AAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCT                                             >  SRR4302542.172611/1‑94 (MQ=60)
                                                AAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTC                                            <  SRR4302542.3321785/94‑1 (MQ=60)
                                                AAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTC                                            >  SRR4302542.4874544/1‑94 (MQ=60)
                                                 AACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCA                                           >  SRR4302542.1723561/1‑94 (MQ=60)
                                                  ACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAA                                          <  SRR4302542.6762192/94‑1 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                         <  SRR4302542.3805603/94‑1 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                         >  SRR4302542.2581015/1‑94 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                         >  SRR4302542.2327486/1‑94 (MQ=60)
                                                   CGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAG                                         <  SRR4302542.578967/94‑1 (MQ=60)
                                                    GAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGG                                        <  SRR4302542.3691993/94‑1 (MQ=60)
                                                    GAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGG                                        <  SRR4302542.7533138/94‑1 (MQ=60)
                                                    GAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGG                                        >  SRR4302542.3619688/1‑94 (MQ=60)
                                                      AAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTA                                      <  SRR4302542.3786779/94‑1 (MQ=60)
                                                      AAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTA                                      >  SRR4302542.3573157/1‑94 (MQ=60)
                                                       AAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAG                                     >  SRR4302542.4214755/1‑94 (MQ=60)
                                                        AAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGC                                    >  SRR4302542.2287282/1‑94 (MQ=60)
                                                        AAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGC                                    >  SRR4302542.5248396/1‑94 (MQ=60)
                                                        AAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGC                                    >  SRR4302542.3545777/1‑94 (MQ=60)
                                                        AAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGC                                    >  SRR4302542.6825971/1‑94 (MQ=60)
                                                         AAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCG                                   >  SRR4302542.4889323/1‑94 (MQ=60)
                                                         AAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCG                                   >  SRR4302542.5077378/1‑94 (MQ=60)
                                                         AAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCG                                   >  SRR4302542.7425150/1‑94 (MQ=60)
                                                           GACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTG                                 >  SRR4302542.1289166/1‑94 (MQ=60)
                                                            ACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGT                                >  SRR4302542.1070570/1‑94 (MQ=60)
                                                             CGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTC                               <  SRR4302542.2547843/94‑1 (MQ=60)
                                                              GCTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCT                              <  SRR4302542.1742097/94‑1 (MQ=60)
                                                               CTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTA                             >  SRR4302542.2581965/1‑94 (MQ=60)
                                                               CTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTA                             >  SRR4302542.6755981/1‑94 (MQ=60)
                                                               CTTTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTA                             >  SRR4302542.2123308/1‑94 (MQ=60)
                                                               CTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTA                             >  SRR4302542.5708692/1‑94 (MQ=60)
                                                                TTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAC                            >  SRR4302542.4638314/1‑94 (MQ=60)
                                                                TTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAC                            >  SRR4302542.1079682/1‑94 (MQ=60)
                                                                 TTTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACC                           >  SRR4302542.5950874/1‑94 (MQ=60)
                                                                  TTCAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCA                          >  SRR4302542.1015809/1‑94 (MQ=60)
                                                                    CAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAT                        >  SRR4302542.3150406/1‑94 (MQ=60)
                                                                    CAGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAT                        >  SRR4302542.3190576/1‑94 (MQ=60)
                                                                    CAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAT                        <  SRR4302542.7073826/94‑1 (MQ=60)
                                                                     AGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATT                       >  SRR4302542.5655215/1‑94 (MQ=60)
                                                                     AGCGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATT                       >  SRR4302542.2297096/1‑94 (MQ=60)
                                                                       CGTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCC                     >  SRR4302542.828570/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    <  SRR4302542.1429201/94‑1 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    >  SRR4302542.7208094/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    >  SRR4302542.598935/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    >  SRR4302542.5253019/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    >  SRR4302542.1859553/1‑94 (MQ=60)
                                                                        GTCTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCA                    <  SRR4302542.6944455/94‑1 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.1096256/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.1096257/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.2097263/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.3267874/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.529669/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.4284156/1‑94 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  <  SRR4302542.3921071/94‑1 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  <  SRR4302542.2201515/94‑1 (MQ=60)
                                                                          CTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACC                  >  SRR4302542.1782115/1‑94 (MQ=60)
                                                                            CTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCAC                >  SRR4302542.792106/1‑94 (MQ=60)
                                                                            CTTTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCAC                <  SRR4302542.5905480/94‑1 (MQ=60)
                                                                            CTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCAC                >  SRR4302542.1613391/1‑94 (MQ=60)
                                                                              TTTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCT              >  SRR4302542.1116129/1‑94 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC             <  SRR4302542.7537755/94‑1 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC             <  SRR4302542.6644327/94‑1 (MQ=60)
                                                                               TTCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTC             >  SRR4302542.6020232/1‑94 (MQ=60)
                                                                                TCTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG            <  SRR4302542.2512718/94‑1 (MQ=60)
                                                                                TCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG            >  SRR4302542.5327505/1‑94 (MQ=60)
                                                                                TCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG            <  SRR4302542.1588184/94‑1 (MQ=60)
                                                                                TCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG            >  SRR4302542.7411656/1‑94 (MQ=60)
                                                                                TCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCG            <  SRR4302542.336096/94‑1 (MQ=60)
                                                                                 CTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGG           >  SRR4302542.6989189/1‑94 (MQ=60)
                                                                                 CTGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGG           >  SRR4302542.7203324/1‑94 (MQ=60)
                                                                                  TGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGC          >  SRR4302542.6527174/1‑94 (MQ=60)
                                                                                  TGGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGC          >  SRR4302542.3406345/1‑94 (MQ=60)
                                                                                   GGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCA         <  SRR4302542.1406047/94‑1 (MQ=60)
                                                                                   GGAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCA         <  SRR4302542.6354165/94‑1 (MQ=60)
                                                                                    GAATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAC        >  SRR4302542.576852/1‑94 (MQ=60)
                                                                                    GAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCAC        <  SRR4302542.1537919/94‑1 (MQ=60)
                                                                                     AATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACG       >  SRR4302542.3165561/1‑94 (MQ=60)
                                                                                      ATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGG      >  SRR4302542.523799/1‑94 (MQ=60)
                                                                                      ATTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGG      >  SRR4302542.3136680/1‑94 (MQ=60)
                                                                                       TTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGA     >  SRR4302542.1690201/1‑94 (MQ=60)
                                                                                       TTTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGA     >  SRR4302542.2175480/1‑94 (MQ=60)
                                                                                        TTGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAT    <  SRR4302542.1351449/94‑1 (MQ=60)
                                                                                         TGGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATA   >  SRR4302542.2457275/1‑94 (MQ=60)
                                                                                          GGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC  <  SRR4302542.1605681/94‑1 (MQ=60)
                                                                                          GGTGCCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC  >  SRR4302542.5527580/1‑94 (MQ=60)
                                                                                             |                                                                                          
ACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATAC  >  NC_000913/3319624‑3319807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑