Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,455,292	T→C	W427R (TGG→CGG)	b3325 →	YheF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,455,292	0	T	C	85.1%	620.1 / 92.3	268	W427R (TGG→CGG)	b3325	YheF
Reads supporting (aligned to +/- strand): ref base T (21/19); new base C (100/128); total (121/147)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 270 total aligned reads displayed.

AAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCC  >  NC_000913/3455202‑3455384

aaaaaGAATGGTGGGATCACCAGCGCGGATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCgg                                                                                            >  2:6422025/1‑93 (MQ=255)

aaaaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTgg                                                                                            <  2:2254181/93‑1 (MQ=255)

aaaaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCgg                                                                                            <  1:4888306/93‑1 (MQ=255)

aaaaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCgg                                                                                            <  2:1259933/93‑1 (MQ=255)

aaaaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCgg                                                                                            >  2:528247/1‑93 (MQ=255)

 aaaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCggg                                                                                           >  2:3779638/1‑93 (MQ=255)

   aaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGa                                                                                         <  1:1458081/93‑1 (MQ=255)

   aaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGa                                                                                         <  2:6002723/93‑1 (MQ=255)

   aaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGa                                                                                         <  1:7376622/93‑1 (MQ=255)

    aGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAg                                                                                        >  1:2216653/1‑93 (MQ=255)

    aGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAg                                                                                        <  2:3075175/93‑1 (MQ=255)

     gAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGt                                                                                       >  2:5725982/1‑93 (MQ=255)

     gAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGt                                                                                       <  1:2158892/93‑1 (MQ=255)

     gAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGt                                                                                       >  2:3653089/1‑93 (MQ=255)

      aaTGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTa                                                                                      <  2:4988070/93‑1 (MQ=255)

       aTGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTAc                                                                                     >  2:3384798/1‑93 (MQ=255)

        tggtggGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACt                                                                                    <  1:3067244/93‑1 (MQ=255)

         ggtggGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTg                                                                                   >  2:1030180/1‑93 (MQ=255)

          gtggGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGc                                                                                  <  2:2961612/93‑1 (MQ=255)

            gggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCtt                                                                                <  2:924767/93‑1 (MQ=255)

              gATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTAc                                                                              <  2:6170875/93‑1 (MQ=255)

                tCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACcg                                                                            >  2:5839207/1‑93 (MQ=255)

                 cACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACcgc                                                                           <  2:2085397/93‑1 (MQ=255)

                    cAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCt                                                                        <  1:473269/93‑1 (MQ=255)

                     aGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTg                                                                       <  1:6090721/93‑1 (MQ=255)

                      gcgcGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTCCCGCGCTgg                                                                      <  1:2372512/93‑1 (MQ=255)

                           aaTCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGt                                                                 >  1:1554283/1‑93 (MQ=255)

                           aaTCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGt                                                                 <  1:3560078/93‑1 (MQ=255)

                            aTCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTa                                                                >  1:1056268/1‑93 (MQ=255)

                             tCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTaa                                                               <  1:3286537/93‑1 (MQ=255)

                             tCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTaa                                                               <  2:4641781/93‑1 (MQ=255)

                             tCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTaa                                                               <  2:5446896/93‑1 (MQ=255)

                             tCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTaa                                                               <  1:6346015/93‑1 (MQ=255)

                              cctgcctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAAc                                                              <  2:2639639/93‑1 (MQ=255)

                                 gcctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAAt                                                           <  1:533002/93‑1 (MQ=255)

                                 gcctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAAt                                                           <  1:871571/93‑1 (MQ=255)

                                   ctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                         <  1:5888758/93‑1 (MQ=255)

                                   ctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                         <  2:5668613/93‑1 (MQ=255)

                                   ctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                         >  2:2500263/1‑93 (MQ=255)

                                   ctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                         >  1:7417471/1‑93 (MQ=255)

                                   ctgGGATATGTTTAGCGCCTACAATAGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                         >  1:2890205/1‑93 (MQ=255)

                                    tgGGATATGTTTAGCGCCTACAATGGCATGTCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaa                                                        <  2:3240545/93‑1 (MQ=255)

                                    tgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaa                                                        >  1:731267/1‑93 (MQ=255)

                                     gggATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                       >  1:290644/1‑93 (MQ=255)

                                     gggATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                       >  1:5715885/1‑93 (MQ=255)

                                      ggATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaaa                                                      >  2:1177101/1‑93 (MQ=255)

                                       gATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAt                                                     >  1:3929180/1‑93 (MQ=255)

                                        atatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATg                                                    <  1:1370883/93‑1 (MQ=255)

                                        atatGTTTAGCCCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGGCCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATg                                                    <  1:3717005/93‑1 (MQ=255)

                                         tatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGa                                                   <  2:4815516/93‑1 (MQ=255)

                                         tatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGa                                                   <  1:3230718/93‑1 (MQ=255)

                                         tatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGa                                                   >  2:1841356/1‑93 (MQ=255)

                                          atGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGAc                                                  <  1:496269/93‑1 (MQ=255)

                                          atGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGAc                                                  <  1:7000764/93‑1 (MQ=255)

                                          atGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGAc                                                  <  1:5207570/93‑1 (MQ=255)

                                            gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                <  1:4264345/93‑1 (MQ=255)

                                            gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                <  2:3272884/93‑1 (MQ=255)

                                               tAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCt                                             <  2:3465366/93‑1 (MQ=255)

                                                aGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTc                                            <  2:6902688/93‑1 (MQ=255)

                                                 gcgcCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                           >  2:1909803/1‑93 (MQ=255)

                                                 gcgcCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                           >  2:7117342/1‑93 (MQ=255)

                                                  cgcCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGc                                          <  2:5230404/93‑1 (MQ=255)

                                                     cttcAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGTTGGCCAGTAACAATAAAAATGACATCCTCGCCAc                                       <  1:4322683/90‑1 (MQ=255)

                                                      tACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCAcc                                      >  2:6309529/1‑93 (MQ=255)

                                                       aCAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCAccc                                     >  2:2420716/1‑93 (MQ=255)

                                                          atggcatggcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCaa                                  >  2:6777971/1‑93 (MQ=255)

                                                          atggcatggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCaa                                  <  1:1333205/93‑1 (MQ=255)

                                                             gcatggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCa                               <  1:1762726/93‑1 (MQ=255)

                                                             gcatggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCa                               >  2:1731057/1‑93 (MQ=255)

                                                              catggcCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAt                              <  2:800652/93‑1 (MQ=255)

                                                              catggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAt                              <  2:3894060/93‑1 (MQ=255)

                                                              catggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAt                              <  2:4477635/93‑1 (MQ=255)

                                                                tggcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCg                            >  1:6032096/1‑93 (MQ=255)

                                                                  gcCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTa                          >  2:4164869/1‑93 (MQ=255)

                                                                    cGCAGGCTTCTTCAATGGGGACCGGGGAGTACTGCTTACCGAGCTGGCCAGTAACAATAAAAATCACATCCTCGCCGCCCCCAGCATCGTAAc                        >  1:6174796/1‑93 (MQ=255)

                                                                    cGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAAc                        <  1:4551488/93‑1 (MQ=255)

                                                                     gCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                       >  1:3997256/1‑93 (MQ=255)

                                                                     gCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                       >  1:1843618/1‑93 (MQ=255)

                                                                     gCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                       >  1:574390/1‑93 (MQ=255)

                                                                       aGGCTTCTACAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCt                     <  2:5628351/93‑1 (MQ=255)

                                                                        ggCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTg                    <  2:856624/93‑1 (MQ=255)

                                                                          cttcttCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGa                  <  2:6612303/93‑1 (MQ=255)

                                                                            tcttcAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGata                >  2:2108913/1‑93 (MQ=255)

                                                                            tcttcAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGata                <  2:437915/93‑1 (MQ=255)

                                                                            tcttcAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGata                <  1:2663058/93‑1 (MQ=255)

                                                                             cttcAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGataa               <  2:132503/93‑1 (MQ=255)

                                                                              ttcAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGataat              >  1:6495452/1‑93 (MQ=255)

                                                                              ttcAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGataat              <  2:2772101/93‑1 (MQ=255)

                                                                              ttcAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGataat              >  1:2435207/1‑93 (MQ=255)

                                                                                 aaTGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATaaa           <  1:1180997/93‑1 (MQ=255)

                                                                                 aaTGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATaaa           <  2:829330/93‑1 (MQ=255)

                                                                                  aTGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAc          >  2:2557065/1‑93 (MQ=255)

                                                                                  aTGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAc          >  2:7295077/1‑93 (MQ=255)

                                                                                  aTGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAc          >  2:453242/1‑93 (MQ=255)

                                                                                   tGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACt         <  1:5554533/93‑1 (MQ=255)

                                                                                   tGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACt         <  1:988728/93‑1 (MQ=255)

                                                                                     gCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcg       <  1:4146592/93‑1 (MQ=255)

                                                                                      cGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcgc      <  1:6935697/93‑1 (MQ=255)

                                                                                      cGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcgc      <  2:7159234/93‑1 (MQ=255)

                                                                                          tGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTcc  >  1:5801598/1‑93 (MQ=255)

AAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCC  >  NC_000913/3455202‑3455384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  NC_000913/3455199‑3455385

TATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACT                                                                                               <  SRR4302542.5438248/94‑1 (MQ=60)

TATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACT                                                                                               <  SRR4302542.78396/94‑1 (MQ=60)

TATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACC                                                                                               >  SRR4302542.7642/1‑94 (MQ=60)

 ATAAAAAGAATGGTGGGATCACTAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCG                                                                                              >  SRR4302542.643343/1‑94 (MQ=60)

 ATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCG                                                                                              >  SRR4302542.1004684/1‑94 (MQ=60)

 ATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCG                                                                                              <  SRR4302542.1853869/94‑1 (MQ=60)

  TAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGG                                                                                             <  SRR4302542.4113542/94‑1 (MQ=60)

   AAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGG                                                                                            <  SRR4302542.4935858/94‑1 (MQ=60)

   AAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGG                                                                                            <  SRR4302542.4935859/94‑1 (MQ=60)

      AAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAG                                                                                         >  SRR4302542.2235727/1‑94 (MQ=60)

      AAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAG                                                                                         <  SRR4302542.1470665/94‑1 (MQ=60)

      AAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAG                                                                                         <  SRR4302542.7448853/94‑1 (MQ=60)

       AGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGT                                                                                        <  SRR4302542.7493201/94‑1 (MQ=60)

        GAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTA                                                                                       <  SRR4302542.2177484/94‑1 (MQ=60)

         AATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTAC                                                                                      <  SRR4302542.156266/94‑1 (MQ=60)

          ATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACT                                                                                     >  SRR4302542.6182766/1‑94 (MQ=60)

          ATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACT                                                                                     <  SRR4302542.1237853/94‑1 (MQ=60)

           TGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTG                                                                                    <  SRR4302542.3096489/94‑1 (MQ=60)

           TGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTG                                                                                    <  SRR4302542.2895498/94‑1 (MQ=60)

              TGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTT                                                                                 >  SRR4302542.3471720/1‑94 (MQ=60)

                 GATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACC                                                                              <  SRR4302542.245368/94‑1 (MQ=60)

                 GATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACC                                                                              <  SRR4302542.2186484/94‑1 (MQ=60)

                  ATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCG                                                                             >  SRR4302542.546305/1‑94 (MQ=60)

                     ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGC                                                                          >  SRR4302542.3135283/1‑94 (MQ=60)

                     ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGC                                                                          >  SRR4302542.7007786/1‑94 (MQ=60)

                     ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGC                                                                          >  SRR4302542.6462956/1‑94 (MQ=60)

                     ACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGC                                                                          >  SRR4302542.4971012/1‑94 (MQ=60)

                       CAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTG                                                                        <  SRR4302542.477362/94‑1 (MQ=60)

                       CAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTG                                                                        <  SRR4302542.2471682/94‑1 (MQ=60)

                       CAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTG                                                                        >  SRR4302542.1780735/1‑94 (MQ=60)

                        AGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGG                                                                       <  SRR4302542.6149684/94‑1 (MQ=60)

                        AGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGG                                                                       >  SRR4302542.7104321/1‑94 (MQ=60)

                        AGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGG                                                                       >  SRR4302542.4909339/1‑94 (MQ=60)

                         GCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTCCCGCGCTGGC                                                                      <  SRR4302542.2393447/94‑1 (MQ=60)

                          CGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCC                                                                     <  SRR4302542.1108675/94‑1 (MQ=60)

                           GCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATAGCGACCGGGGAGTACTGCTTACCGCGCTGGCCA                                                                    >  SRR4302542.1379131/1‑94 (MQ=60)

                             GAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGT                                                                  >  SRR4302542.1567698/1‑94 (MQ=60)

                              AATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTA                                                                 >  SRR4302542.1065345/1‑94 (MQ=60)

                              AATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTA                                                                 <  SRR4302542.3593320/94‑1 (MQ=60)

                               ATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAA                                                                >  SRR4302542.4739216/1‑94 (MQ=60)

                               ATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAA                                                                <  SRR4302542.1418890/94‑1 (MQ=60)

                                TCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAAC                                                               <  SRR4302542.3317567/94‑1 (MQ=60)

                                TCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAAC                                                               <  SRR4302542.3317568/94‑1 (MQ=60)

                                TCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAAC                                                               <  SRR4302542.6408536/94‑1 (MQ=60)

                                 CCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACA                                                              <  SRR4302542.934453/94‑1 (MQ=60)

                                   TGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAAT                                                            <  SRR4302542.7484832/94‑1 (MQ=60)

                                   TGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAAT                                                            >  SRR4302542.2347882/1‑94 (MQ=60)

                                    GCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATA                                                           <  SRR4302542.537606/94‑1 (MQ=60)

                                    GCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATA                                                           <  SRR4302542.879083/94‑1 (MQ=60)

                                     CCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAA                                                          >  SRR4302542.2222331/1‑94 (MQ=60)

                                     CCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAA                                                          >  SRR4302542.7490052/1‑94 (MQ=60)

                                     CCTGGGATATGTTTAGCGCCTACAATAGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAA                                                          >  SRR4302542.2917955/1‑94 (MQ=60)

                                     CCTGGGAAATTTTTACCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGCTTGGGGAGTACTGCTTCCCGCGCTGGCCAGTAACAATAA                                                          <  SRR4302542.7505659/94‑1 (MQ=60)

                                      CTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAA                                                         <  SRR4302542.5945827/94‑1 (MQ=60)

                                      CTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAA                                                         >  SRR4302542.2242703/1‑94 (MQ=60)

                                      CTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAA                                                         >  SRR4302542.737598/1‑94 (MQ=60)

                                       TGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAA                                                        >  SRR4302542.293183/1‑94 (MQ=60)

                                       TGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAA                                                        >  SRR4302542.6076631/1‑94 (MQ=60)

                                       TGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAA                                                        >  SRR4302542.5771361/1‑94 (MQ=60)

                                         GGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAT                                                      <  SRR4302542.4384243/94‑1 (MQ=60)

                                         GGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAT                                                      >  SRR4302542.724583/1‑94 (MQ=60)

                                         GGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAT                                                      >  SRR4302542.3967949/1‑94 (MQ=60)

                                         GGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAT                                                      <  SRR4302542.5039283/94‑1 (MQ=60)

                                         GGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAAT                                                      <  SRR4302542.1341251/94‑1 (MQ=60)

                                          GATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATG                                                     >  SRR4302542.3005497/1‑94 (MQ=60)

                                           ATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGA                                                    <  SRR4302542.1382736/94‑1 (MQ=60)

                                           ATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGA                                                    >  SRR4302542.1004315/1‑94 (MQ=60)

                                           ATATGTTTAGCCCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGGCCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGA                                                    <  SRR4302542.3753775/94‑1 (MQ=60)

                                            TATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGAC                                                   <  SRR4302542.3261278/94‑1 (MQ=60)

                                             ATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACA                                                  <  SRR4302542.500543/94‑1 (MQ=60)

                                             ATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACA                                                  <  SRR4302542.5258219/94‑1 (MQ=60)

                                             ATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACA                                                  <  SRR4302542.7069535/94‑1 (MQ=60)

                                               GTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATC                                                <  SRR4302542.4306316/94‑1 (MQ=60)

                                                 TTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCT                                              <  SRR4302542.4057734/94‑1 (MQ=60)

                                                  TAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTC                                             <  SRR4302542.5863800/94‑1 (MQ=60)

                                                   AGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCG                                            <  SRR4302542.3718404/94‑1 (MQ=60)

                                                   AGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCAGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCG                                            >  SRR4302542.6138275/1‑94 (MQ=60)

                                                    GCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGC                                           <  SRR4302542.6306687/94‑1 (MQ=60)

                                                     CGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCC                                          <  SRR4302542.205864/94‑1 (MQ=60)

                                                       CCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCAC                                        <  SRR4302542.5845494/94‑1 (MQ=60)

                                                        CTTCAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGTTGGCCAGTAACAATAAAAATGACATCCTCGCCACC                                       <  SRR4302542.4365182/94‑1 (MQ=60)

                                                        CTACAATGGCATGGCCGCAGTCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACC                                       >  SRR4302542.4117740/1‑94 (MQ=60)

                                                        CTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACC                                       >  SRR4302542.324547/1‑94 (MQ=60)

                                                         TACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCC                                      >  SRR4302542.4222651/1‑94 (MQ=60)

                                                          ACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCC                                     >  SRR4302542.6362380/1‑94 (MQ=60)

                                                           CAATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCA                                    <  SRR4302542.5345968/94‑1 (MQ=60)

                                                            AATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGGACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAA                                   >  SRR4302542.3372792/1‑94 (MQ=60)

                                                             ATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAG                                  <  SRR4302542.1344691/94‑1 (MQ=60)

                                                             ATGGCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAG                                  <  SRR4302542.5774184/94‑1 (MQ=60)

                                                                GCATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAT                               <  SRR4302542.1778016/94‑1 (MQ=60)

                                                                 CATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATC                              <  SRR4302542.5013884/94‑1 (MQ=60)

                                                                  ATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCG                             >  SRR4302542.6090497/1‑94 (MQ=60)

                                                                  ATGGCCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCG                             >  SRR4302542.5318439/1‑94 (MQ=60)

                                                                   TGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGT                            <  SRR4302542.575369/94‑1 (MQ=60)

                                                                      CCGCAGGCTTCTTCAATGGGGACCGGGGAGTACTGCTTACCGAGCTGGCCAGTAACAATAAAAATCACATCCTCGCCGCCCCCAGCATCGTAAC                         >  SRR4302542.6234651/1‑94 (MQ=60)

                                                                      CCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAAC                         <  SRR4302542.2970478/94‑1 (MQ=60)

                                                                      CCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAAC                         <  SRR4302542.4033064/94‑1 (MQ=60)

                                                                      CCGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAAC                         <  SRR4302542.4430281/94‑1 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.4036685/1‑94 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.205439/1‑94 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.1859570/1‑94 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.1607140/1‑94 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        <  SRR4302542.1488530/94‑1 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        <  SRR4302542.4596031/94‑1 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.579322/1‑94 (MQ=60)

                                                                       CGCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACG                        >  SRR4302542.3620797/1‑94 (MQ=60)

                                                                        GCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGC                       >  SRR4302542.5778752/1‑94 (MQ=60)

                                                                        GCAGGCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGC                       >  SRR4302542.5706356/1‑94 (MQ=60)

                                                                            GCTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGA                   >  SRR4302542.4777230/1‑94 (MQ=60)

                                                                             CTTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGAT                  >  SRR4302542.4197230/1‑94 (MQ=60)

                                                                              TTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATA                 >  SRR4302542.7468328/1‑94 (MQ=60)

                                                                              TTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATA                 >  SRR4302542.4322589/1‑94 (MQ=60)

                                                                              TTCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATA                 <  SRR4302542.6189964/94‑1 (MQ=60)

                                                                               TCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAA                <  SRR4302542.3798787/94‑1 (MQ=60)

                                                                               TCTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAA                <  SRR4302542.2688760/94‑1 (MQ=60)

                                                                                CTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAAT               >  SRR4302542.7438018/1‑94 (MQ=60)

                                                                                CTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAAT               >  SRR4302542.6559465/1‑94 (MQ=60)

                                                                                CTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCGCCCCAAGCATCGTAACGCTGGATAAT               <  SRR4302542.702575/94‑1 (MQ=60)

                                                                                CTTCAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAAT               >  SRR4302542.2458900/1‑94 (MQ=60)

                                                                                   CAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAA            >  SRR4302542.6457194/1‑94 (MQ=60)

                                                                                   CAATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGGAACGCTGGATAATAAA            >  SRR4302542.2920569/1‑94 (MQ=60)

                                                                                    AATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAC           <  SRR4302542.1191193/94‑1 (MQ=60)

                                                                                    AATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAC           >  SRR4302542.5977411/1‑94 (MQ=60)

                                                                                    AATGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAC           <  SRR4302542.6657676/94‑1 (MQ=60)

                                                                                      TGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTC         <  SRR4302542.997244/94‑1 (MQ=60)

                                                                                      TGGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTC         <  SRR4302542.5608501/94‑1 (MQ=60)

                                                                                       GGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCG        <  SRR4302542.5863630/94‑1 (MQ=60)

                                                                                       GGCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCG        <  SRR4302542.1372212/94‑1 (MQ=60)

                                                                                        GCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGC       <  SRR4302542.2515711/94‑1 (MQ=60)

                                                                                        GCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGC       <  SRR4302542.4187439/94‑1 (MQ=60)

                                                                                        GCGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGC       <  SRR4302542.393503/94‑1 (MQ=60)

                                                                                         CGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCG      <  SRR4302542.7003836/94‑1 (MQ=60)

                                                                                         CGACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCG      >  SRR4302542.1618204/1‑94 (MQ=60)

                                                                                          GACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGT     >  SRR4302542.1070228/1‑94 (MQ=60)

                                                                                           ACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTC    <  SRR4302542.5403159/94‑1 (MQ=60)

                                                                                           ACCGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTC    >  SRR4302542.3371765/1‑94 (MQ=60)

                                                                                            CTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCC   >  SRR4302542.5857899/1‑94 (MQ=60)

                                                                                            CTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCC   >  SRR4302542.2851565/1‑94 (MQ=60)

                                                                                             TGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  SRR4302542.1673897/1‑94 (MQ=60)

                                                                                             CGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  SRR4302542.3926861/1‑94 (MQ=60)

                                                                                             CGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  SRR4302542.9499/1‑94 (MQ=60)

                                                                                             CGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  SRR4302542.2969137/1‑94 (MQ=60)

TATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCT  >  NC_000913/3455199‑3455385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`