Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,458,811	C→T	A51V (GCG→GTG)	hofG →	putative general protein secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,458,811	0	C	T	82.3%	677.7 / 103.0	273	A51V (GCG→GTG)	hofG	putative general protein secretion protein
Reads supporting (aligned to +/- strand): ref base C (33/15); new base T (114/110); total (148/125)
Fisher's exact test for biased strand distribution p-value = 2.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

Only 100 of 279 total aligned reads displayed.

TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACAC  >  NC_000913/3458721‑3458903

ttATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGgtgc                                                                                            >  1:5002562/1‑93 (MQ=255)

ttATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGgtgc                                                                                            >  1:7060420/1‑93 (MQ=255)

 tATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCt                                                                                           >  1:7296427/1‑93 (MQ=255)

  aTCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTg                                                                                          >  2:5856936/1‑93 (MQ=255)

      gCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGaaa                                                                                      >  2:751789/1‑93 (MQ=255)

      gCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGaaa                                                                                      <  2:4560777/93‑1 (MQ=255)

       cGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGaaaa                                                                                     >  2:7337679/1‑93 (MQ=255)

        gTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAc                                                                                    <  2:5944072/93‑1 (MQ=255)

         tACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACg                                                                                   >  2:2429844/1‑93 (MQ=255)

          aCTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGc                                                                                  >  1:6178942/1‑93 (MQ=255)

           cTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGcc                                                                                 <  1:1041982/93‑1 (MQ=255)

           cTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGcc                                                                                 >  1:1810528/1‑93 (MQ=255)

            ttGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGccc                                                                                >  2:2204005/1‑93 (MQ=255)

            ttGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGccc                                                                                >  1:4640621/1‑93 (MQ=255)

              gCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCtt                                                                              <  2:937766/93‑1 (MQ=255)

                cAGCGTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGa                                                                            <  2:3632639/93‑1 (MQ=255)

                cAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGa                                                                            <  1:73847/93‑1 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                           <  1:168692/93‑1 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                           >  1:1465703/1‑93 (MQ=255)

                  gCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGata                                                                          <  2:2837564/93‑1 (MQ=255)

                  gCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGata                                                                          >  2:325677/1‑93 (MQ=255)

                    cTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATg                                                                        >  2:4847368/1‑93 (MQ=255)

                     tggtggTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGt                                                                       >  1:1109331/1‑93 (MQ=255)

                     tggtggTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGt                                                                       <  1:6545417/93‑1 (MQ=255)

                           ttCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAAc                                                                 >  2:5960395/1‑93 (MQ=255)

                               taacctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGa                                                             >  2:3812609/1‑93 (MQ=255)

                                  cctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACaa                                                          <  2:4807972/93‑1 (MQ=255)

                                  cctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACaa                                                          >  2:2054109/1‑93 (MQ=255)

                                  cctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACaa                                                          <  1:1633414/93‑1 (MQ=255)

                                   ctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                                         <  2:4115011/93‑1 (MQ=255)

                                   ctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                                         >  1:2052285/1‑93 (MQ=255)

                                   ctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                                         >  1:6905621/1‑93 (MQ=255)

                                    taaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAGCTCGACAatc                                                        <  2:29244/93‑1 (MQ=255)

                                    taaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatc                                                        <  1:2540691/93‑1 (MQ=255)

                                      aTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGNNNNNGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatcat                                                      <  2:6063739/93‑1 (MQ=255)

                                        gggCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                                                    <  1:6148519/93‑1 (MQ=255)

                                           cAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTa                                                 <  2:6645602/93‑1 (MQ=255)

                                            aaTAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAt                                                >  1:2784244/1‑93 (MQ=255)

                                             aTAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATc                                               <  2:3101441/93‑1 (MQ=255)

                                             aTAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATc                                               >  1:804902/1‑93 (MQ=255)

                                              tAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATcc                                              >  2:5380337/1‑93 (MQ=255)

                                              tAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATcc                                              >  2:4345253/1‑93 (MQ=255)

                                              tAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATcc                                              >  1:4080234/1‑93 (MQ=255)

                                                aaGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCaa                                            >  1:2287162/1‑93 (MQ=255)

                                                   aaaaGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACca                                         >  2:6874498/1‑93 (MQ=255)

                                                    aaaGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACcac                                        >  1:5514717/1‑93 (MQ=255)

                                                    aaaGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACcac                                        >  2:3723188/1‑93 (MQ=255)

                                                     aaGGCGGATAAGCAAAAAGCCGTCAACGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACcaca                                       <  2:737891/93‑1 (MQ=255)

                                                       ggCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaaa                                     >  1:6466453/1‑93 (MQ=255)

                                                       ggCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaaa                                     <  2:6577218/93‑1 (MQ=255)

                                                       ggCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaaa                                     >  2:1079938/1‑93 (MQ=255)

                                                        gCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAAt                                    <  1:380495/93‑1 (MQ=255)

                                                         cGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATc                                   <  1:3107874/93‑1 (MQ=255)

                                                         cGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATc                                   <  1:1519497/93‑1 (MQ=255)

                                                          ggATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCa                                  >  2:1151688/1‑93 (MQ=255)

                                                          ggATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCa                                  <  2:1484011/93‑1 (MQ=255)

                                                           gATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAg                                 >  2:6299371/1‑93 (MQ=255)

                                                           gATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAg                                 >  1:625837/1‑93 (MQ=255)

                                                            aTAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgg                                >  2:2615474/1‑93 (MQ=255)

                                                             tAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAggg                               <  1:3155922/93‑1 (MQ=255)

                                                             tAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAggg                               >  1:6754772/1‑93 (MQ=255)

                                                             tAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAggg                               <  1:1259184/93‑1 (MQ=255)

                                                              aaGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgggg                              <  1:4193571/93‑1 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                            <  1:5509216/93‑1 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                            >  2:3513426/1‑93 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                            >  2:514433/1‑93 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAAACAaggggt                            >  2:5537436/1‑91 (MQ=255)

                                                                 cAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt                           <  2:1860313/93‑1 (MQ=255)

                                                                 cAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt                           <  1:2137064/93‑1 (MQ=255)

                                                                  aaaaaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTg                          >  2:7116171/1‑93 (MQ=255)

                                                                  aaaaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTg                          >  2:494510/1‑93 (MQ=255)

                                                                   aaaaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGa                         >  1:2835803/1‑93 (MQ=255)

                                                                   aaaaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGa                         <  2:972535/93‑1 (MQ=255)

                                                                   aaaaGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGa                         >  1:3773228/1‑93 (MQ=255)

                                                                     aaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAAt                       <  1:1345802/93‑1 (MQ=255)

                                                                      aGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATc                      >  1:3425573/1‑93 (MQ=255)

                                                                       gCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATcc                     >  1:3881130/1‑93 (MQ=255)

                                                                         cGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCtt                   <  2:163548/93‑1 (MQ=255)

                                                                         cGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCtt                   >  2:5052602/1‑93 (MQ=255)

                                                                         cGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCtt                   <  2:5679430/93‑1 (MQ=255)

                                                                          gTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta                  <  1:5782680/93‑1 (MQ=255)

                                                                          gTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta                  <  1:4422937/93‑1 (MQ=255)

                                                                          gTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta                  <  2:6472929/93‑1 (MQ=255)

                                                                           tCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtag                 >  1:7007005/1‑93 (MQ=255)

                                                                           tCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtag                 <  1:5263052/93‑1 (MQ=255)

                                                                           tCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtag                 <  1:5217258/93‑1 (MQ=255)

                                                                           tCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtag                 >  1:2717498/1‑93 (MQ=255)

                                                                           tCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTtag                 >  2:6434041/1‑93 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa             <  1:93701/93‑1 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa             <  1:5954968/93‑1 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa             >  1:482645/1‑93 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa             <  1:4686117/93‑1 (MQ=255)

                                                                                 atatTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAg           >  1:4897102/1‑93 (MQ=255)

                                                                                  tatTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgc          <  2:2330568/93‑1 (MQ=255)

                                                                                  tatTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgc          >  1:6063676/1‑93 (MQ=255)

                                                                                  tatTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgc          >  2:3373340/1‑93 (MQ=255)

                                                                                   atTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgcg         >  1:7478034/1‑93 (MQ=255)

                                                                                    ttGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgcgc        >  2:205696/1‑93 (MQ=255)

                                                                                     tgtgGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGcc       <  2:2520750/93‑1 (MQ=255)

                                                                                       tgGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGa     >  1:5350924/1‑93 (MQ=255)

TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACAC  >  NC_000913/3458721‑3458903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  >  NC_000913/3458718‑3458904

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGT                                                                                               <  SRR4302542.4944866/94‑1 (MQ=60)

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGT                                                                                               <  SRR4302542.5017890/94‑1 (MQ=60)

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGT                                                                                               <  SRR4302542.1685087/94‑1 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              <  SRR4302542.3057565/94‑1 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302542.3200799/1‑94 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302542.5051155/1‑94 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302542.7129724/1‑94 (MQ=60)

   TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCT                                                                                            >  SRR4302542.7367929/1‑94 (MQ=60)

        GGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAA                                                                                       >  SRR4302542.3010647/1‑94 (MQ=60)

         GCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAA                                                                                      <  SRR4302542.3869265/94‑1 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     <  SRR4302542.6949956/94‑1 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     <  SRR4302542.3281068/94‑1 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     <  SRR4302542.1159440/94‑1 (MQ=60)

           GTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACG                                                                                    >  SRR4302542.1393528/1‑94 (MQ=60)

           GTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACG                                                                                    >  SRR4302542.840572/1‑94 (MQ=60)

            TACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGC                                                                                   >  SRR4302542.6238856/1‑94 (MQ=60)

             ACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCC                                                                                  >  SRR4302542.6041310/1‑94 (MQ=60)

             ACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCC                                                                                  >  SRR4302542.1826219/1‑94 (MQ=60)

              CTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCC                                                                                 <  SRR4302542.1050928/94‑1 (MQ=60)

              CTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCC                                                                                 >  SRR4302542.4685964/1‑94 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302542.6131038/94‑1 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302542.5309242/94‑1 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302542.3845202/94‑1 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            <  SRR4302542.4178343/94‑1 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            <  SRR4302542.74457/94‑1 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            >  SRR4302542.1478359/1‑94 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           <  SRR4302542.170119/94‑1 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           >  SRR4302542.7111253/1‑94 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           >  SRR4302542.2773739/1‑94 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           >  SRR4302542.7479954/1‑94 (MQ=60)

                     GCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATAT                                                                          <  SRR4302542.2287483/94‑1 (MQ=60)

                      CCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATG                                                                         <  SRR4302542.5033920/94‑1 (MQ=60)

                      CCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATG                                                                         >  SRR4302542.6546283/1‑94 (MQ=60)

                       CTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGT                                                                        >  SRR4302542.1118823/1‑94 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       >  SRR4302542.6144672/1‑94 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       <  SRR4302542.2668512/94‑1 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       <  SRR4302542.5263748/94‑1 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       <  SRR4302542.6609962/94‑1 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTA                                                                       >  SRR4302542.2195676/1‑94 (MQ=60)

                            GGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAA                                                                   >  SRR4302542.4690903/1‑94 (MQ=60)

                             GTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAAC                                                                  >  SRR4302542.5568094/1‑94 (MQ=60)

                              TTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACT                                                                 <  SRR4302542.4016793/94‑1 (MQ=60)

                               TCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTC                                                                <  SRR4302542.7580848/94‑1 (MQ=60)

                                cttactgTAATGGGCAATAAAGAAAAGGCGGATAAGAAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCG                                                               <  SRR4302542.5759270/87‑1 (MQ=60)

                                CCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCG                                                               >  SRR4302542.4696781/1‑94 (MQ=60)

                                  TAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGAC                                                             <  SRR4302542.1691990/94‑1 (MQ=60)

                                   AACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACA                                                            <  SRR4302542.2469595/94‑1 (MQ=60)

                                   AACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACA                                                            >  SRR4302542.5910434/1‑94 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          >  SRR4302542.2070024/1‑94 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          <  SRR4302542.1647502/94‑1 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          <  SRR4302542.3717969/94‑1 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          <  SRR4302542.6212530/94‑1 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          >  SRR4302542.6973416/1‑94 (MQ=60)

                                       TAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCA                                                        <  SRR4302542.2565322/94‑1 (MQ=60)

                                        AATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCAT                                                       >  SRR4302542.5902295/1‑94 (MQ=60)

                                         ATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATC                                                      >  SRR4302542.4833938/1‑94 (MQ=60)

                                          TGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCA                                                     <  SRR4302542.5383882/94‑1 (MQ=60)

                                          TGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCA                                                     <  SRR4302542.6805694/94‑1 (MQ=60)

                                          TGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCA                                                     <  SRR4302542.7531973/94‑1 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAC                                                    <  SRR4302542.6208010/94‑1 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAC                                                    >  SRR4302542.284085/1‑94 (MQ=60)

                                              CAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAT                                                 >  SRR4302542.2698429/1‑94 (MQ=60)

                                              CAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAT                                                 >  SRR4302542.2811050/1‑94 (MQ=60)

                                               AATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATC                                                >  SRR4302542.811824/1‑94 (MQ=60)

                                               AATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATC                                                <  SRR4302542.5522943/94‑1 (MQ=60)

                                               AATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATC                                                >  SRR4302542.5310945/1‑94 (MQ=60)

                                               AATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATC                                                <  SRR4302542.3180371/94‑1 (MQ=60)

                                                ATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCC                                               <  SRR4302542.6430073/94‑1 (MQ=60)

                                                ATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCC                                               >  SRR4302542.4120447/1‑94 (MQ=60)

                                                 TAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCA                                              >  SRR4302542.448135/1‑94 (MQ=60)

                                                  AAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAA                                             >  SRR4302542.1710035/1‑94 (MQ=60)

                                                  AAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAA                                             >  SRR4302542.4565163/1‑94 (MQ=60)

                                                  AAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAA                                             >  SRR4302542.870381/1‑94 (MQ=60)

                                                  AAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAA                                             >  SRR4302542.2306814/1‑94 (MQ=60)

                                                     GAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCA                                          <  SRR4302542.7110324/94‑1 (MQ=60)

                                                     GAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCA                                          >  SRR4302542.7454513/1‑94 (MQ=60)

                                                      AAAAGGCGGATAAGCACAAAGCAGTCAGCGATATGGAGGAGCTGGAAAAAGCCCTTGATATGTACAAACTAGACACTCATCACTATCCAACCAC                                         >  SRR4302542.4912443/1‑94 (MQ=60)

                                                      AAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCAC                                         >  SRR4302542.5568310/1‑94 (MQ=60)

                                                      AAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCAC                                         <  SRR4302542.1507171/94‑1 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      >  SRR4302542.6530160/1‑94 (MQ=60)

                                                           GCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATC                                    >  SRR4302542.5331932/1‑94 (MQ=60)

                                                           GCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATC                                    <  SRR4302542.383803/94‑1 (MQ=60)

                                                            CGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCA                                   <  SRR4302542.3137416/94‑1 (MQ=60)

                                                            CGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCA                                   <  SRR4302542.1532622/94‑1 (MQ=60)

                                                             GGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAG                                  <  SRR4302542.6391076/94‑1 (MQ=60)

                                                             GGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAG                                  >  SRR4302542.631225/1‑94 (MQ=60)

                                                              GATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGG                                 <  SRR4302542.7217304/94‑1 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                >  SRR4302542.6821221/1‑94 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                >  SRR4302542.7070193/1‑94 (MQ=60)

                                                                TAAGGAAAAACCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGG                               <  SRR4302542.79773/94‑1 (MQ=60)

                                                                TAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGG                               <  SRR4302542.3185883/94‑1 (MQ=60)

                                                                TAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGG                               <  SRR4302542.1270013/94‑1 (MQ=60)

                                                                 AAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGC                              <  SRR4302542.4234918/94‑1 (MQ=60)

                                                                 AAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGC                              >  SRR4302542.7053083/1‑94 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCT                             <  SRR4302542.4820216/94‑1 (MQ=60)

                                                                   GCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTT                            <  SRR4302542.5562753/94‑1 (MQ=60)

                                                                    CAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTG                           <  SRR4302542.2155506/94‑1 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          <  SRR4302542.1378224/94‑1 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          >  SRR4302542.2863063/1‑94 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          >  SRR4302542.2071437/1‑94 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          >  SRR4302542.3810547/1‑94 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTGGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          >  SRR4302542.6277975/1‑94 (MQ=60)

                                                                      AAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAA                         <  SRR4302542.180706/94‑1 (MQ=60)

                                                                      AAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAA                         >  SRR4302542.1112443/1‑94 (MQ=60)

                                                                       AAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGGTATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAAT                        >  SRR4302542.6277238/1‑94 (MQ=60)

                                                                        AAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATC                       >  SRR4302542.3457657/1‑94 (MQ=60)

                                                                        AAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATC                       <  SRR4302542.1357383/94‑1 (MQ=60)

                                                                         AGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCC                      <  SRR4302542.6922499/94‑1 (MQ=60)

                                                                         AGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCC                      >  SRR4302542.3919464/1‑94 (MQ=60)

                                                                         AGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCC                      <  SRR4302542.316093/94‑1 (MQ=60)

                                                                          GCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCT                     <  SRR4302542.7187265/94‑1 (MQ=60)

                                                                           CCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTT                    <  SRR4302542.2971256/94‑1 (MQ=60)

                                                                            CGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTA                   >  SRR4302542.490785/1‑94 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  >  SRR4302542.7075838/1‑94 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  >  SRR4302542.2743705/1‑94 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302542.5838830/94‑1 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302542.4466376/94‑1 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  >  SRR4302542.3516939/1‑94 (MQ=60)

                                                                             GTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302542.4222085/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACTCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.2790988/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.5314198/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.6022035/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 >  SRR4302542.1645862/1‑94 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.5267993/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.3822549/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302542.5132720/94‑1 (MQ=60)

                                                                               CAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTA                >  SRR4302542.1356752/1‑94 (MQ=60)

                                                                                AGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAGCTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAG               <  SRR4302542.4397393/94‑1 (MQ=60)

                                                                                AGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAG               <  SRR4302542.5484590/94‑1 (MQ=60)

                                                                                 GCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGA              >  SRR4302542.486827/1‑94 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302542.94492/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             >  SRR4302542.1557268/1‑94 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302542.6012634/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302542.1664899/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302542.4731887/94‑1 (MQ=60)

                                                                                   GATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAG            <  SRR4302542.3982756/94‑1 (MQ=60)

                                                                                   GATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAG            >  SRR4302542.4944706/1‑94 (MQ=60)

                                                                                    ATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGC           >  SRR4302542.1950926/1‑94 (MQ=60)

                                                                                    ATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGC           >  SRR4302542.6122373/1‑94 (MQ=60)

                                                                                     TATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCG          >  SRR4302542.5831834/1‑94 (MQ=60)

                                                                                     TATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCG          <  SRR4302542.6533044/94‑1 (MQ=60)

                                                                                     TATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCG          <  SRR4302542.1188214/94‑1 (MQ=60)

                                                                                     TATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCG          >  SRR4302542.7551322/1‑94 (MQ=60)

                                                                                     TATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCG          >  SRR4302542.186398/1‑94 (MQ=60)

                                                                                      ATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         <  SRR4302542.2559100/94‑1 (MQ=60)

                                                                                      ATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         >  SRR4302542.1297339/1‑94 (MQ=60)

                                                                                         GTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGA      >  SRR4302542.5402899/1‑94 (MQ=60)

                                                                                         GTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGA      >  SRR4302542.5755772/1‑94 (MQ=60)

                                                                                            GTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACAC   >  SRR4302542.5777100/1‑94 (MQ=60)

                                                                                             TGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  <  SRR4302542.4775477/94‑1 (MQ=60)

                                                                                             TGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  <  SRR4302542.1381080/94‑1 (MQ=60)

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  >  NC_000913/3458718‑3458904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`