Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,500,011 G→A R12Q (CGG→CAG)  b3372 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,500,0110GA82.4% 731.2 / 108.6 284R12Q (CGG→CAG) b3372hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/16);  new base A (124/110);  total (158/126)
Fisher's exact test for biased strand distribution p-value = 6.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 294 total aligned reads displayed.

ATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGCGCCGG  >  NC_000913/3499921‑3500103
                                                                                          |                                                                                            
atatTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCagcagc                                                                                            <  2:5073981/93‑1 (MQ=255)
 tatTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCt                                                                                           >  2:1645008/1‑93 (MQ=255)
  atTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTg                                                                                          >  1:646072/1‑93 (MQ=255)
   ttAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGc                                                                                         <  1:1052523/93‑1 (MQ=255)
   ttAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGc                                                                                         <  1:5336635/93‑1 (MQ=255)
    tAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGcc                                                                                        >  1:7279137/1‑93 (MQ=255)
    tAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGcc                                                                                        <  1:4363124/93‑1 (MQ=255)
       tCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTAt                                                                                     >  1:7358621/1‑93 (MQ=255)
       tCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTAt                                                                                     <  1:2022972/93‑1 (MQ=255)
       tCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTAt                                                                                     >  1:6330867/1‑93 (MQ=255)
          ctctCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGa                                                                                  >  2:1002131/1‑93 (MQ=255)
          ctctCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGa                                                                                  >  1:6019962/1‑93 (MQ=255)
          ctctCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGa                                                                                  <  2:3412599/93‑1 (MQ=255)
           tctcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGaa                                                                                 >  2:937608/1‑93 (MQ=255)
           tctcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGaa                                                                                 <  2:2448663/93‑1 (MQ=255)
           tctcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGaa                                                                                 >  2:5997738/1‑93 (MQ=255)
           tctcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGaa                                                                                 >  1:3902299/1‑93 (MQ=255)
             tcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAaca                                                                               >  1:4578817/1‑93 (MQ=255)
             tcCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAaca                                                                               >  2:1754282/1‑93 (MQ=255)
              cccggcccggCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAacac                                                                              >  1:1622588/1‑93 (MQ=255)
               ccggcccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACg                                                                             >  2:5821966/1‑93 (MQ=255)
               ccggcccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACg                                                                             >  1:4972150/1‑93 (MQ=255)
                cggcccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGc                                                                            >  1:2962771/1‑93 (MQ=255)
                cggcccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGc                                                                            >  2:2707709/1‑93 (MQ=255)
                 ggcccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCa                                                                           <  1:6135640/93‑1 (MQ=255)
                   cccggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCAtt                                                                         <  2:1217368/93‑1 (MQ=255)
                     cggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTc                                                                       <  1:1388808/93‑1 (MQ=255)
                     cggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTc                                                                       >  2:2956838/1‑93 (MQ=255)
                     cggcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTc                                                                       >  1:6738642/1‑93 (MQ=255)
                       gcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGt                                                                     >  2:3747737/1‑93 (MQ=255)
                       gcAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGt                                                                     <  1:6690669/93‑1 (MQ=255)
                         aaCGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGa                                                                   >  2:6122791/1‑93 (MQ=255)
                         aaCGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGa                                                                   <  1:5480049/93‑1 (MQ=255)
                            gggCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGc                                                                <  1:3708294/93‑1 (MQ=255)
                             ggCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCa                                                               <  1:6408530/93‑1 (MQ=255)
                              gCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCaa                                                              >  2:1832488/1‑93 (MQ=255)
                              gCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCaa                                                              <  2:7192256/93‑1 (MQ=255)
                               ccGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAg                                                             >  1:3788902/1‑93 (MQ=255)
                               ccGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAg                                                             <  1:6188583/93‑1 (MQ=255)
                               ccGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAg                                                             <  1:5009130/93‑1 (MQ=255)
                               ccGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAg                                                             <  1:4755926/93‑1 (MQ=255)
                               ccGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAg                                                             <  1:2666623/93‑1 (MQ=255)
                                  gATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGa                                                          <  1:3403797/93‑1 (MQ=255)
                                    tttACGCAAGGAGTTACCCGATGCAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGc                                                        >  2:171194/1‑93 (MQ=255)
                                    tttACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGc                                                        <  2:6216221/93‑1 (MQ=255)
                                    tttACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGc                                                        >  1:6267833/1‑93 (MQ=255)
                                     ttACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCAAGCt                                                       <  2:7374193/93‑1 (MQ=255)
                                      tACGCAAGGAGTTCCCCGAGGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTg                                                      <  1:6929298/93‑1 (MQ=255)
                                      tACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTg                                                      <  2:5344948/93‑1 (MQ=255)
                                       aCGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTgg                                                     >  2:2055606/1‑93 (MQ=255)
                                       aCGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTgg                                                     <  1:3241902/93‑1 (MQ=255)
                                           aaGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTa                                                 >  2:1304031/1‑93 (MQ=255)
                                           aaGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTa                                                 <  2:1391476/93‑1 (MQ=255)
                                            aGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTac                                                >  2:6301001/1‑93 (MQ=255)
                                             ggAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTacg                                               <  1:3957485/93‑1 (MQ=255)
                                              gAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTacga                                              <  2:6429364/93‑1 (MQ=255)
                                              gAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTacga                                              >  2:3011016/1‑93 (MQ=255)
                                                gTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTacgacg                                            >  2:2565943/1‑93 (MQ=255)
                                                gTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTacgacg                                            <  1:6645390/93‑1 (MQ=255)
                                                  tACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGc                                          >  2:4897161/1‑93 (MQ=255)
                                                  tACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTATGACGGc                                          <  2:4721141/93‑1 (MQ=255)
                                                   aCCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCa                                         <  1:2506795/93‑1 (MQ=255)
                                                     ccGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATc                                       >  2:5914734/1‑93 (MQ=255)
                                                       gATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGa                                     >  1:210605/1‑93 (MQ=255)
                                                       gATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGa                                     >  1:1229273/1‑93 (MQ=255)
                                                       gATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGa                                     >  1:7443491/1‑93 (MQ=255)
                                                         tGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGaaa                                   >  2:3082856/1‑93 (MQ=255)
                                                          gAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAAt                                  <  1:1030357/93‑1 (MQ=255)
                                                           aaaaCAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAAtt                                 <  2:6612675/93‑1 (MQ=255)
                                                            aaaCAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAAttt                                >  2:4852046/1‑93 (MQ=255)
                                                             aaCAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTg                               >  2:5497695/1‑93 (MQ=255)
                                                              aCAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTgg                              <  2:3642127/93‑1 (MQ=255)
                                                               cAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTggg                             >  2:5162476/1‑93 (MQ=255)
                                                                aGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTgggg                            >  2:4607990/1‑93 (MQ=255)
                                                                 ggTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGc                           >  2:4684817/1‑93 (MQ=255)
                                                                 ggTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGc                           >  2:321514/1‑93 (MQ=255)
                                                                   tATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCgg                         <  1:5628586/93‑1 (MQ=255)
                                                                    aTGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGt                        >  1:5587925/1‑93 (MQ=255)
                                                                    aTGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGt                        >  1:5849232/1‑93 (MQ=255)
                                                                     tGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTc                       >  2:2208537/1‑93 (MQ=255)
                                                                        ttACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGcc                    >  2:4868114/1‑93 (MQ=255)
                                                                        ttACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGcc                    >  1:1201650/1‑93 (MQ=255)
                                                                         tACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGccc                   <  2:3952501/93‑1 (MQ=255)
                                                                         tACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGccc                   <  2:6051340/93‑1 (MQ=255)
                                                                          aCCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCg                  >  2:3135229/1‑93 (MQ=255)
                                                                           ccTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGc                 >  1:1876657/1‑93 (MQ=255)
                                                                            cTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCa                >  2:2360656/1‑93 (MQ=255)
                                                                            cTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCa                <  1:3584180/93‑1 (MQ=255)
                                                                             tGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCAc               >  2:5655498/1‑93 (MQ=255)
                                                                              gCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCAcg              <  1:858700/93‑1 (MQ=255)
                                                                                  ccaccaGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGtt          <  1:4175187/93‑1 (MQ=255)
                                                                                   caccaGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTc         >  2:4304742/1‑93 (MQ=255)
                                                                                   caccaGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTc         <  2:7432266/93‑1 (MQ=255)
                                                                                    accaGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTcg        >  1:7113433/1‑93 (MQ=255)
                                                                                    accaGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTcg        >  2:3366602/1‑93 (MQ=255)
                                                                                      cagcagCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTcgtg      >  1:7188143/1‑91 (MQ=255)
                                                                                      cagcagCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTcgtg      >  1:2292821/1‑91 (MQ=255)
                                                                                       agcagcTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCgtgc     <  2:3614224/93‑1 (MQ=255)
                                                                                       agcagcTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCgtgc     <  2:6742979/93‑1 (MQ=255)
                                                                                          ggCTGCCCATTGAACACGCATTTCGTGCGGCAACCGAGCTGGGTTACGACGGCCTCGAAATTTGGGGCGGTCGCCCGCACGCGGTCGCGCCgg  >  1:4451652/1‑93 (MQ=255)
                                                                                          |                                                                                            
ATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGCGCCGG  >  NC_000913/3499921‑3500103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGCGCCGGA  >  NC_000913/3499918‑3500104
                                                                                              |                                                                                             
GGAATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCA                                                                                               <  SRR4302542.5999497/94‑1 (MQ=60)
GGAATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCA                                                                                               >  SRR4302542.300742/1‑94 (MQ=60)
GGAATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCA                                                                                               >  SRR4302542.3533055/1‑94 (MQ=60)
   ATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCT                                                                                            <  SRR4302542.6222717/94‑1 (MQ=60)
    TATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTG                                                                                           >  SRR4302542.651623/1‑94 (MQ=60)
    TATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTG                                                                                           <  SRR4302542.4213366/94‑1 (MQ=60)
      TTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCC                                                                                         <  SRR4302542.1061576/94‑1 (MQ=60)
      TTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCC                                                                                         >  SRR4302542.7350524/1‑94 (MQ=60)
      TTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCC                                                                                         <  SRR4302542.5388485/94‑1 (MQ=60)
       TAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCT                                                                                        <  SRR4302542.4405963/94‑1 (MQ=60)
       TAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCT                                                                                        >  SRR4302542.3025702/1‑94 (MQ=60)
       TAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCT                                                                                        <  SRR4302542.5517088/94‑1 (MQ=60)
        AAGCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTA                                                                                       <  SRR4302542.3921914/94‑1 (MQ=60)
         ATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTAT                                                                                      <  SRR4302542.5696079/94‑1 (MQ=60)
         ATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTAT                                                                                      >  SRR4302542.7430701/1‑94 (MQ=60)
         ATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTAT                                                                                      >  SRR4302542.6393246/1‑94 (MQ=60)
          TCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATT                                                                                     >  SRR4302542.7528342/1‑94 (MQ=60)
          TCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATT                                                                                     <  SRR4302542.2040467/94‑1 (MQ=60)
           CCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTG                                                                                    >  SRR4302542.5661817/1‑94 (MQ=60)
           CCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTG                                                                                    >  SRR4302542.1189095/1‑94 (MQ=60)
           CCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGAATGTTTACCTGCGGCCACCAGCAGCTGCCTATTG                                                                                    >  SRR4302542.3860774/1‑94 (MQ=60)
            CCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGA                                                                                   >  SRR4302542.6078266/1‑94 (MQ=60)
             CTCTTCCCGGCCCGGCAACGGGCCGGTTTTACGCAAGGAGTTACCCGATGAAAACAGGTTTGTTTAACTGCGGGCACCAGCAGGTGCCCATTGA                                                                                   >  SRR4302542.2407197/1‑94 (MQ=60)
             CTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCCGCCTATTGAA                                                                                  <  SRR4302542.5910821/94‑1 (MQ=60)
             CTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAA                                                                                  >  SRR4302542.3565758/1‑94 (MQ=60)
             CTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAA                                                                                  >  SRR4302542.3940817/1‑94 (MQ=60)
               C‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACA                                                                                >  SRR4302542.1699120/1‑94 (MQ=60)
               C‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACA                                                                                >  SRR4302542.5517948/1‑94 (MQ=60)
               C‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACA                                                                                >  SRR4302542.4623610/1‑94 (MQ=60)
                 TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACAC                                                                               >  SRR4302542.737968/1‑94 (MQ=60)
                 TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACAC                                                                               >  SRR4302542.1636602/1‑94 (MQ=60)
                  CCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACG                                                                              >  SRR4302542.5020420/1‑94 (MQ=60)
                   CGGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGC                                                                             >  SRR4302542.6249715/1‑94 (MQ=60)
                   CCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGC                                                                             >  SRR4302542.2991160/1‑94 (MQ=60)
                     GGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCAT                                                                           <  SRR4302542.6040839/94‑1 (MQ=60)
                     GGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCAT                                                                           <  SRR4302542.6195003/94‑1 (MQ=60)
                      GCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATT                                                                          <  SRR4302542.5569877/94‑1 (MQ=60)
                      GCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATT                                                                          <  SRR4302542.7305678/94‑1 (MQ=60)
                       CCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTT                                                                         <  SRR4302542.7050917/94‑1 (MQ=60)
                       CCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTT                                                                         >  SRR4302542.2851544/1‑94 (MQ=60)
                       CCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTT                                                                         >  SRR4302542.520880/1‑94 (MQ=60)
                        CCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTC                                                                        >  SRR4302542.6804969/1‑94 (MQ=60)
                         CGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCG                                                                       <  SRR4302542.1400800/94‑1 (MQ=60)
                         CGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGACTATTGAACACGCATTTCG                                                                       <  SRR4302542.4507651/94‑1 (MQ=60)
                          GGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGT                                                                      >  SRR4302542.2100340/1‑94 (MQ=60)
                           GCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTG                                                                     <  SRR4302542.6713216/94‑1 (MQ=60)
                           GCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTG                                                                     <  SRR4302542.6756577/94‑1 (MQ=60)
                           GCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTG                                                                     <  SRR4302542.4472981/94‑1 (MQ=60)
                            CAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGA                                                                    >  SRR4302542.4476906/1‑94 (MQ=60)
                            CAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGA                                                                    >  SRR4302542.1643473/1‑94 (MQ=60)
                            CAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGA                                                                    >  SRR4302542.224822/1‑94 (MQ=60)
                             AACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGAT                                                                   <  SRR4302542.5533277/94‑1 (MQ=60)
                             AACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTAAT                                                                   >  SRR4302542.1393646/1‑94 (MQ=60)
                              ACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATG                                                                  >  SRR4302542.5681562/1‑94 (MQ=60)
                                GGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCA                                                                >  SRR4302542.3644557/1‑94 (MQ=60)
                                GGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCA                                                                <  SRR4302542.3744990/94‑1 (MQ=60)
                                GGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCA                                                                >  SRR4302542.6518616/1‑94 (MQ=60)
                                 GGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAA                                                               <  SRR4302542.7364469/94‑1 (MQ=60)
                                 GGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAA                                                               >  SRR4302542.3422833/1‑94 (MQ=60)
                                 GGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAA                                                               <  SRR4302542.6471678/94‑1 (MQ=60)
                                  GCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAG                                                              >  SRR4302542.3826363/1‑94 (MQ=60)
                                  GCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAG                                                              >  SRR4302542.1233106/1‑94 (MQ=60)
                                  GCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAG                                                              <  SRR4302542.4541933/94‑1 (MQ=60)
                                   CCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGC                                                             <  SRR4302542.6248722/94‑1 (MQ=60)
                                   CCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGC                                                             <  SRR4302542.4802313/94‑1 (MQ=60)
                                   CCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGC                                                             <  SRR4302542.2692366/94‑1 (MQ=60)
                                   CCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGC                                                             <  SRR4302542.5057782/94‑1 (MQ=60)
                                     GGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGA                                                           >  SRR4302542.1051737/1‑94 (MQ=60)
                                     GGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGA                                                           >  SRR4302542.4818962/1‑94 (MQ=60)
                                     GGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGA                                                           >  SRR4302542.5536493/1‑94 (MQ=60)
                                      GATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAG                                                          >  SRR4302542.1543348/1‑94 (MQ=60)
                                      GATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAG                                                          >  SRR4302542.6168595/1‑94 (MQ=60)
                                      GATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAG                                                          <  SRR4302542.3435712/94‑1 (MQ=60)
                                       ATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGC                                                         <  SRR4302542.852075/94‑1 (MQ=60)
                                       ATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGC                                                         >  SRR4302542.4229447/1‑94 (MQ=60)
                                       ATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGC                                                         >  SRR4302542.6329613/1‑94 (MQ=60)
                                        TTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCT                                                        <  SRR4302542.93357/94‑1 (MQ=60)
                                        TTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCT                                                        <  SRR4302542.6993816/94‑1 (MQ=60)
                                        TTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCT                                                        >  SRR4302542.5765916/1‑94 (MQ=60)
                                          TACGCAAGGAGTTCCCCGAGGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGG                                                      <  SRR4302542.6997327/94‑1 (MQ=60)
                                           ACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGG                                                     >  SRR4302542.6609539/1‑94 (MQ=60)
                                           ACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGG                                                     <  SRR4302542.3272547/94‑1 (MQ=60)
                                            CGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGT                                                    >  SRR4302542.5027568/1‑94 (MQ=60)
                                              CAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTA                                                  >  SRR4302542.4692747/1‑94 (MQ=60)
                                              CAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTA                                                  <  SRR4302542.4090265/94‑1 (MQ=60)
                                              CAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTA                                                  >  SRR4302542.1447136/1‑94 (MQ=60)
                                               AAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTAC                                                 >  SRR4302542.1212973/1‑94 (MQ=60)
                                                AGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACG                                                <  SRR4302542.633109/94‑1 (MQ=60)
                                                 GGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGA                                               <  SRR4302542.3996555/94‑1 (MQ=60)
                                                  GAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGAC                                              <  SRR4302542.2300330/94‑1 (MQ=60)
                                                   AGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACG                                             <  SRR4302542.2349118/94‑1 (MQ=60)
                                                    GTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGG                                            <  SRR4302542.6779313/94‑1 (MQ=60)
                                                    GTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGG                                            <  SRR4302542.6710885/94‑1 (MQ=60)
                                                      TACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCA                                          <  SRR4302542.50266/94‑1 (MQ=60)
                                                      TACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCA                                          >  SRR4302542.2028675/1‑94 (MQ=60)
                                                       ACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCAT                                         <  SRR4302542.3951358/94‑1 (MQ=60)
                                                       ACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCAT                                         >  SRR4302542.342917/1‑94 (MQ=60)
                                                       ACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCAT                                         <  SRR4302542.2531126/94‑1 (MQ=60)
                                                          CGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGA                                      >  SRR4302542.212419/1‑94 (MQ=60)
                                                          CGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGA                                      >  SRR4302542.260874/1‑94 (MQ=60)
                                                          CGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGA                                      >  SRR4302542.1239846/1‑94 (MQ=60)
                                                          CGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGA                                      >  SRR4302542.7516372/1‑94 (MQ=60)
                                                           GATGAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAA                                     >  SRR4302542.7333958/1‑94 (MQ=60)
                                                            ATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAA                                    >  SRR4302542.5202410/1‑94 (MQ=60)
                                                             TGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAAT                                   >  SRR4302542.2593962/1‑94 (MQ=60)
                                                              GAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATT                                  <  SRR4302542.4877297/94‑1 (MQ=60)
                                                              GAAAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATT                                  <  SRR4302542.1039229/94‑1 (MQ=60)
                                                              GAAAACAGGGATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATT                                  >  SRR4302542.3699469/1‑94 (MQ=60)
                                                                AAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTG                                <  SRR4302542.3818859/94‑1 (MQ=60)
                                                                AAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTG                                <  SRR4302542.2086752/94‑1 (MQ=60)
                                                                AAACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTG                                >  SRR4302542.3946087/1‑94 (MQ=60)
                                                                 AACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGG                               <  SRR4302542.6771901/94‑1 (MQ=60)
                                                                  ACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGG                              <  SRR4302542.7442798/94‑1 (MQ=60)
                                                                  ACAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGG                              <  SRR4302542.4679467/94‑1 (MQ=60)
                                                                   CAGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGG                             <  SRR4302542.6834194/94‑1 (MQ=60)
                                                                    AGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGC                            <  SRR4302542.7465208/94‑1 (MQ=60)
                                                                    AGGTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGC                            >  SRR4302542.6747138/1‑94 (MQ=60)
                                                                      GTATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGG                          >  SRR4302542.6409488/1‑94 (MQ=60)
                                                                       TATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGT                         >  SRR4302542.5642222/1‑94 (MQ=60)
                                                                       TATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGT                         <  SRR4302542.5683235/94‑1 (MQ=60)
                                                                       TATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGT                         >  SRR4302542.6611636/1‑94 (MQ=60)
                                                                       TATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGT                         >  SRR4302542.5905959/1‑94 (MQ=60)
                                                                        ATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTC                        >  SRR4302542.3449244/1‑94 (MQ=60)
                                                                        ATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTC                        >  SRR4302542.7025840/1‑94 (MQ=60)
                                                                        ATGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTC                        >  SRR4302542.7232369/1‑94 (MQ=60)
                                                                         TGTTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCG                       >  SRR4302542.6503929/1‑94 (MQ=60)
                                                                           TTTACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCC                     >  SRR4302542.1211993/1‑94 (MQ=60)
                                                                             TACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCG                   <  SRR4302542.6372694/94‑1 (MQ=60)
                                                                             TACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCG                   >  SRR4302542.6432995/1‑94 (MQ=60)
                                                                              ACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGC                  >  SRR4302542.7193124/1‑94 (MQ=60)
                                                                              ACCTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGC                  >  SRR4302542.1892898/1‑94 (MQ=60)
                                                                                CTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCAC                <  SRR4302542.3617611/94‑1 (MQ=60)
                                                                                CTGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCAC                >  SRR4302542.6319325/1‑94 (MQ=60)
                                                                                 TGCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACG               <  SRR4302542.3074603/94‑1 (MQ=60)
                                                                                  GCGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGC              <  SRR4302542.866098/94‑1 (MQ=60)
                                                                                   CGGCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCG             >  SRR4302542.2745340/1‑94 (MQ=60)
                                                                                     GCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTT           <  SRR4302542.1948601/94‑1 (MQ=60)
                                                                                     GCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTT           <  SRR4302542.1071739/94‑1 (MQ=60)
                                                                                     GCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTT           >  SRR4302542.6168457/1‑94 (MQ=60)
                                                                                     GCCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTT           >  SRR4302542.4336926/1‑94 (MQ=60)
                                                                                      CCACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTC          <  SRR4302542.4216363/94‑1 (MQ=60)
                                                                                       CACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCG         >  SRR4302542.7183262/1‑94 (MQ=60)
                                                                                        ACCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGT        >  SRR4302542.126210/1‑94 (MQ=60)
                                                                                         CCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGTG       >  SRR4302542.7258635/1‑94 (MQ=60)
                                                                                         CCAGCAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGTG       >  SRR4302542.2312516/1‑94 (MQ=60)
                                                                                             CGGCTGCCCATTGAACACGCATTTCGTGCGGCAACCGAGCTGGGTTACGACGGCCTCGAAATTTGGGGCGGTCGCCCGCACGCGGTCGCGCCGG   >  SRR4302542.4495319/1‑94 (MQ=60)
                                                                                             CAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGGCCGCACGCGTTCGCGCCGG   >  SRR4302542.2970999/1‑94 (MQ=60)
                                                                                             CAGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGTGCCGG   <  SRR4302542.2064515/94‑1 (MQ=60)
                                                                                              AGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGTGCCGGA  >  SRR4302542.5002997/1‑94 (MQ=60)
                                                                                              |                                                                                             
GGAATATTAATCCCTC‑TCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCACCAGCGGCTGCCTATTGAACACGCATTTCGTGATGCAAGCGAGCTGGGTTACGACGGCATCGAAATTTGGGGCGGTCGCCCGCACGCGTTCGCGCCGGA  >  NC_000913/3499918‑3500104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑